AT4G11450

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006314
Physical Location & Seq
Reverse (-)
6958472 .. 6962059
3588 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G11450.1

Sequence Viewer

Length: 2085 bp
ATGGAATTTGGTAAAGGAATTGATATGGAACAAAGCCCCAATAGTGTTCTTCCTCCTCCTCGTCCATTCACTGAATCAGAAAAGAGGACTAGTACAAGACTAAAGCCGCCTCGTAGAGATGAGATATTGAGAGTCAAAGAAGGTTTCACCGAGATAAGTTTCAGGCGTTACCGTAGCACATCATGTAAGAACTTTCCTTCTAAACCACTTGCAATGGGAGATACAACGGAGCTAAGGCGCGGTTCTGTTTATCAAAGCTCTAATGAGTTGTTTAAAGAACTTAGAGAACCACAAGGACGTAAAGATTCAAAGGCGAAACTTGAGTTGTCTCGTAGTAGTGATGCTTCTTTCTCCTTTAGAGTTGTTGATTCTTCGAGGAAGGGAAGTACTGAGAAAAGACCGCATAAGACGGTTTTAGATGGTCAGAAGTCATTAGTTGAACCTTGCACCTCTAGCAACTTCATTGACATTTGTTTGAAGTCAGGTATTAAAGATAGAGCTGTAGTGTTGGATTCAGATGATAATGACCATAAGATTAGATTGCCTAAGCCATATTCTGCAAAGGTAGACTCAGTAGAAACCAGTTACAATAAGGACAGCTCAAGAGTTAGGAAAATGTTTGATCCATTTGTTAAATCAAAATCTTTGAGAAGTCCTCTTGGTTATATAGGAGAATCAGGTGATCAGTTCAAATTGGGGAGGAACAGTGAGCGTTGCAGATCTATGTTGAGTGACTACTCAAACATTCATAAAAGATCGAATCTTTGTCCTCCTCCAGTTGTCAATAAGGATTATACCTCGGTGCTGAAATCTTCCCCGGTTCATCTACATTGCCGTCTCAAGATGGAAAGCAAGAATGGATTACCGGTTTTTCAGTTTGTTTCAGATTCTCCTGAGGAAGTTTACGCTGCAAAGACGTGGAAATCTGACAATGGTTCTACTTGGGTATATACATTTTCATCTGCTGGAAGCAGAAAGAGGAGCAGTGCTAGCGTGCGGGGTTTGACCGATGTTAGTAAAGAATCTTTGCTGGTGGCTCAGATGCAAGTTACCTGTAAGATGTGTTCAGAAGTAAGAAAAAAGGGACAAGATCCAGAAACTTTGATGGTGAATGAGTTTGTTTTGTATGATATTGCACAAGCAAGAAGAAGTGTCTCCACAAAGGAAGATCAATCACTACCACTTGATACGGTTAATAATGCCTCCAAGAACTCTGTTAAATCAGACTCAGAGATAAGAAATAACTCAATGTCCGGGGATGCTTCGGATACAATGAAGCAGAGATCTCAGCCTAAAAGAACATCTCAAAGCTATGATCTTGAGGCTTCAAATGGAACAAACCCGTGGTCAGCTGCAAATTTGCATCCTGACCTTGAGATCGCAGCTATAATTATTCAAGACACTATTGAGAAGAGAGAAAGCTTAAAATATAGAAGAGGTGACAAAAGACTGATGGAGAAAACTAATATTCTTGGTCTTTCTCCAATAGAAGAGGAGAAAAAGGAGTTGTTTGGCAGCAGAAGTTTAGAAAAACTGAAGGTTGTAATCCCGAGGGGAAACCACGGGTTACCAACTACCGAAAACTCTTGTCCTTCGCCTTTGATTCAGCGATGGAGGTCAGGAGGCGGTTGTGACTGTGGTGGTTGGGACATGGCTTGTCCACTTATGGTTCTTGGTAATCCTCGTATCTCATGTTCTCATGATCAGCCTCTTGTAGAAAATCAGCATCCTTTGCAACTATTTGTCCAGGGTGCTAAAGAACATATACCAGCATTGTACATGTCATTTGTTGAGGAAGGACAGTACGATGTTCATTTCCACGCGCAGTTATCAACATTGCAAGCATTCTCCATCTGTGTTGCCATATTACATAACACGGAAGTCTCAGATAGTTACAGAAATGGCGAGAATGTACAACAGTTTTCGCATTGCAACTCGTTGAAAATGCTGATTGATGATGATGTTCAGTTCTTAGTTGAAGCAGTAACAGAGGAAGAAGAAGTCCCTAAGCCTCTGAAAGAAGCTGTGACCGCTCTTCAATCCTACATGCCGAATCCTCCTTTCTCGCCAATCTCCCGAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3527

Protein Analysis

694

Amino Acids

77.98

Weight (kDa)

8.75

Isoelectric Point (pI)

65.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 273 - 625 1.1e-118 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 2033
AccI GTMKAC 1 cut(s) 567
AccII CGCG 2 cut(s) 240, 1823
AciI CCGC 6 cut(s) 107, 240, 401, 997, 1626, 2031
AclWI GGATC 2 cut(s) 617, 1085
AcsI RAATTY 2 cut(s) 5, 1357
AcuI CTGAAG 1 cut(s) 1556
AfaI GTAC 5 cut(s) 94, 388, 1778, 1805, 1914
AflIII ACRYGT 1 cut(s) 1779
AgeI ACCGGT 1 cut(s) 865
AgsI TTSAA 9 cut(s) 309, 440, 478, 691, 1329, 1397, 1942, 1979, 2039
AhlI ACTAGT 1 cut(s) 89
AjiI CACGTC 1 cut(s) 918
AjnI CCWGG 1 cut(s) 1746
AluBI AGCT 9 cut(s) 232, 258, 500, 600, 1311, 1352, 1385, 1422, 2024
AluI AGCT 9 cut(s) 232, 258, 500, 600, 1311, 1352, 1385, 1422, 2024
Alw26I GTCTC 4 cut(s) 333, 842, 1159, 1888
AlwI GGATC 2 cut(s) 617, 1085
Ama87I CYCGRG 2 cut(s) 1549, 2076
ApeKI GCWGC 4 cut(s) 908, 1352, 1382, 1515
ApoI RAATTY 2 cut(s) 5, 1357
ArsI GACNNNNNNTTYG 2 cut(s) 1331, 1363
AsiGI ACCGGT 1 cut(s) 865
AspLEI GCGC 2 cut(s) 240, 1825
AsuC2I CCSGG 2 cut(s) 818, 1255
AsuHPI GGTGA 4 cut(s) 139, 692, 1120, 1451
AsuNHI GCTAGC 1 cut(s) 989
AvaI CYCGRG 2 cut(s) 1549, 2076
AxyI CCTNAGG 1 cut(s) 894
BarI GAAGNNNNNNTAC 2 cut(s) 328, 360
BbvI GCAGC 4 cut(s) 895, 1339, 1394, 1527
BccI CCATC 6 cut(s) 413, 838, 1099, 1447, 1605, 1859
BceAI ACGGC 1 cut(s) 819
BciT130I CCWGG 1 cut(s) 1748
BciVI GTATCC 1 cut(s) 1261
BclI TGATCA 2 cut(s) 682, 1702
BcnI CCSGG 2 cut(s) 818, 1255
BcoDI GTCTC 4 cut(s) 333, 842, 1159, 1888
BcuI ACTAGT 1 cut(s) 89
BfaI CTAG 3 cut(s) 90, 453, 990
BfmI CTRYAG 1 cut(s) 501
BfuI GTATCC 1 cut(s) 1261
BglII AGATCT 2 cut(s) 719, 1283
BisI GCNGC 5 cut(s) 107, 909, 1353, 1383, 1516
BlsI GCNGC 5 cut(s) 108, 910, 1354, 1384, 1517
BmcAI AGTACT 1 cut(s) 388
Bme1390I CCNGG 3 cut(s) 818, 1255, 1748
BmeT110I CYCGRG 2 cut(s) 1549, 2076
BmgBI CACGTC 1 cut(s) 918
BmrFI CCNGG 3 cut(s) 818, 1255, 1748
BmsI GCATC 5 cut(s) 331, 1032, 1249, 1372, 1735
BmtI GCTAGC 1 cut(s) 993
BplI GAGNNNNNCTC 2 cut(s) 640, 672
BpmI CTGGAG 1 cut(s) 759
Bpu10I CCTNAGC 3 cut(s) 233, 546, 2007
BpuEI CTTGAG 5 cut(s) 341, 586, 824, 1340, 1394
BpuMI CCSGG 2 cut(s) 818, 1255
BsaJI CCNNGG 7 cut(s) 798, 816, 1254, 1343, 1550, 1561, 1747
BsaWI WCCGGW 1 cut(s) 865
BsaXI ACNNNNNCTCC 2 cut(s) 691, 721
Bse118I RCCGGY 1 cut(s) 865
Bse1I ACTGG 2 cut(s) 582, 776
Bse21I CCTNAGG 1 cut(s) 894
Bse3DI GCAATG 4 cut(s) 219, 829, 1835, 1927
BseBI CCWGG 1 cut(s) 1748
BseDI CCNNGG 7 cut(s) 798, 816, 1254, 1343, 1550, 1561, 1747
BseGI GGATG 3 cut(s) 1264, 1363, 1726
BseMI GCAATG 4 cut(s) 219, 829, 1835, 1927
BseMII CTCAG 7 cut(s) 381, 585, 885, 1052, 1242, 1301, 1899
BseNI ACTGG 2 cut(s) 582, 776
BseRI GAGGAG 5 cut(s) 45, 48, 762, 994, 1508
BseXI GCAGC 4 cut(s) 895, 1339, 1394, 1527
Bsh1236I CGCG 2 cut(s) 240, 1823
BshTI ACCGGT 1 cut(s) 865
BsiHKCI CYCGRG 2 cut(s) 1549, 2076
BsiSI CCGG 3 cut(s) 818, 866, 1254
BslFI GGGAC 3 cut(s) 1098, 1661, 1988
BsmAI GTCTC 4 cut(s) 333, 842, 1159, 1888
BsmBI CGTCTC 1 cut(s) 842
BsmFI GGGAC 3 cut(s) 1098, 1661, 1988
BsmI GAATGC 1 cut(s) 1844
BsoBI CYCGRG 2 cut(s) 1549, 2076
Bsp1407I TGTACA 2 cut(s) 1776, 1912
BspACI CCGC 6 cut(s) 107, 240, 401, 997, 1626, 2031
BspCNI CTCAG 7 cut(s) 382, 584, 886, 1051, 1241, 1300, 1898
BspFNI CGCG 2 cut(s) 240, 1823
BspHI TCATGA 1 cut(s) 1699
BspOI GCTAGC 1 cut(s) 993
BspPI GGATC 2 cut(s) 617, 1085
BspQI GCTCTTC 1 cut(s) 2040
BsrBI CCGCTC 1 cut(s) 2033
BsrDI GCAATG 4 cut(s) 219, 829, 1835, 1927
BsrFI RCCGGY 1 cut(s) 865
BsrGI TGTACA 2 cut(s) 1776, 1912
BsrI ACTGG 2 cut(s) 582, 776
BssAI RCCGGY 1 cut(s) 865
BssECI CCNNGG 7 cut(s) 798, 816, 1254, 1343, 1550, 1561, 1747
Bst2UI CCWGG 1 cut(s) 1748
Bst4CI ACNGT 7 cut(s) 173, 412, 707, 1192, 1637, 1803, 1920
Bst6I CTCTTC 4 cut(s) 1406, 1429, 1485, 2040
BstAPI GCANNNNNTGC 1 cut(s) 1732
BstAUI TGTACA 2 cut(s) 1776, 1912
BstC8I GCNNGC 3 cut(s) 991, 995, 1842
BstDSI CCRYGG 2 cut(s) 1343, 1561
BstEII GGTNACC 1 cut(s) 1566
BstF5I GGATG 3 cut(s) 1264, 1363, 1726
BstFNI CGCG 2 cut(s) 240, 1823
BstHHI GCGC 2 cut(s) 240, 1825
BstMAI GTCTC 4 cut(s) 333, 842, 1159, 1888
BstMWI GCNNNNNNNGC 4 cut(s) 453, 990, 1732, 2030
BstNI CCWGG 1 cut(s) 1748
BstNSI RCATGY 2 cut(s) 1783, 2050
BstPI GGTNACC 1 cut(s) 1566
BstSCI CCNGG 3 cut(s) 816, 1253, 1746
BstSFI CTRYAG 1 cut(s) 501
BstUI CGCG 2 cut(s) 240, 1823
BstV1I GCAGC 4 cut(s) 895, 1339, 1394, 1527
BstX2I RGATCY 3 cut(s) 719, 1090, 1283
BstYI RGATCY 3 cut(s) 719, 1090, 1283
Bsu36I CCTNAGG 1 cut(s) 894
BsuI GTATCC 1 cut(s) 1261
BtgI CCRYGG 2 cut(s) 1343, 1561
BtgZI GCGATG 1 cut(s) 1624
BtrI CACGTC 1 cut(s) 918
BtsCI GGATG 3 cut(s) 1264, 1363, 1726
BtsI GCAGTG 1 cut(s) 991
BtsIMutI CAGTG 3 cut(s) 69, 712, 991
Cac8I GCNNGC 3 cut(s) 991, 995, 1842
CciI TCATGA 1 cut(s) 1699
CfoI GCGC 2 cut(s) 240, 1825
Cfr10I RCCGGY 1 cut(s) 865
Csp6I GTAC 5 cut(s) 93, 387, 1777, 1804, 1913
CspAI ACCGGT 1 cut(s) 865
CviAII CATG 6 cut(s) 183, 1651, 1692, 1700, 1780, 2047
CviQI GTAC 5 cut(s) 93, 387, 1777, 1804, 1913
DraI TTTAAA 1 cut(s) 274
Eam1104I CTCTTC 4 cut(s) 1406, 1429, 1485, 2040
EarI CTCTTC 4 cut(s) 1406, 1429, 1485, 2040
Eco57I CTGAAG 1 cut(s) 1556
Eco81I CCTNAGG 1 cut(s) 894
Eco88I CYCGRG 2 cut(s) 1549, 2076
Eco91I GGTNACC 1 cut(s) 1566
EcoO65I GGTNACC 1 cut(s) 1566
EcoRII CCWGG 1 cut(s) 1746
Esp3I CGTCTC 1 cut(s) 842
FaeI CATG 6 cut(s) 186, 1654, 1695, 1703, 1783, 2050
FaqI GGGAC 3 cut(s) 1098, 1661, 1988
FatI CATG 6 cut(s) 182, 1650, 1691, 1699, 1779, 2046
FauI CCCGC 1 cut(s) 990
FbaI TGATCA 2 cut(s) 682, 1702
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 5 cut(s) 107, 909, 1353, 1383, 1516
FokI GGATG 3 cut(s) 1271, 1350, 1713
Fsp4HI GCNGC 5 cut(s) 107, 909, 1353, 1383, 1516
FspBI CTAG 3 cut(s) 90, 453, 990
GlaI GCGC 2 cut(s) 239, 1824
GluI GCNGC 5 cut(s) 107, 909, 1353, 1383, 1516
GsuI CTGGAG 1 cut(s) 759
HapII CCGG 3 cut(s) 818, 866, 1254
HhaI GCGC 2 cut(s) 240, 1825
Hin1II CATG 6 cut(s) 186, 1654, 1695, 1703, 1783, 2050
Hin6I GCGC 2 cut(s) 238, 1823
HinP1I GCGC 2 cut(s) 238, 1823
HindIII AAGCTT 1 cut(s) 1420
HpaII CCGG 3 cut(s) 818, 866, 1254
HphI GGTGA 4 cut(s) 139, 692, 1120, 1451
Hpy166II GTNNAC 3 cut(s) 568, 904, 1661
Hpy8I GTNNAC 3 cut(s) 568, 904, 1661
HpyAV CCTTC 6 cut(s) 134, 207, 373, 1531, 1602, 1790
HpyCH4III ACNGT 7 cut(s) 173, 412, 707, 1192, 1637, 1803, 1920
HpyCH4IV ACGT 2 cut(s) 298, 917
HpyF10VI GCNNNNNNNGC 4 cut(s) 453, 990, 1732, 2030
HpySE526I ACGT 2 cut(s) 298, 917
Hsp92II CATG 6 cut(s) 186, 1654, 1695, 1703, 1783, 2050
HspAI GCGC 2 cut(s) 238, 1823
Ksp22I TGATCA 2 cut(s) 682, 1702
LguI GCTCTTC 1 cut(s) 2040
LmnI GCTCC 2 cut(s) 229, 981
Lsp1109I GCAGC 4 cut(s) 895, 1339, 1394, 1527
LweI GCATC 5 cut(s) 331, 1032, 1249, 1372, 1735
MaeI CTAG 3 cut(s) 90, 453, 990
MaeII ACGT 2 cut(s) 298, 917
MbiI CCGCTC 1 cut(s) 2033
MflI RGATCY 3 cut(s) 719, 1090, 1283
MluCI AATT 5 cut(s) 5, 18, 692, 1357, 1389
MlyI GAGTC 3 cut(s) 141, 563, 1220
MmeI TCCRAC 1 cut(s) 489
MseI TTAA 6 cut(s) 273, 489, 633, 1194, 1218, 1424
MspA1I CMGCKG 1 cut(s) 1352
MspI CCGG 3 cut(s) 818, 866, 1254
MspR9I CCNGG 3 cut(s) 818, 1255, 1748
Mva1269I GAATGC 1 cut(s) 1844
MvaI CCWGG 1 cut(s) 1748
MvnI CGCG 2 cut(s) 240, 1823
MwoI GCNNNNNNNGC 4 cut(s) 453, 990, 1732, 2030
NciI CCSGG 2 cut(s) 818, 1255
NheI GCTAGC 1 cut(s) 989
NlaIII CATG 6 cut(s) 186, 1654, 1695, 1703, 1783, 2050
NmuCI GTSAC 4 cut(s) 731, 1439, 1631, 2026
NspI RCATGY 2 cut(s) 1783, 2050
PagI TCATGA 1 cut(s) 1699
PciI ACATGT 1 cut(s) 1779
PciSI GCTCTTC 1 cut(s) 2040
PctI GAATGC 1 cut(s) 1844
PinAI ACCGGT 1 cut(s) 865
PkrI GCNGC 5 cut(s) 108, 910, 1354, 1384, 1517
PleI GAGTC 3 cut(s) 140, 563, 1220
PpsI GAGTC 3 cut(s) 140, 563, 1220
PscI ACATGT 1 cut(s) 1779
Psp6I CCWGG 1 cut(s) 1746
PspEI GGTNACC 1 cut(s) 1566
PspGI CCWGG 1 cut(s) 1746
PsrI GAACNNNNNNTAC 4 cut(s) 1048, 1080, 1678, 1710
PsuI RGATCY 3 cut(s) 719, 1090, 1283
PvuII CAGCTG 1 cut(s) 1352
RsaI GTAC 5 cut(s) 94, 388, 1778, 1805, 1914
RsaNI GTAC 5 cut(s) 93, 387, 1777, 1804, 1913
SapI GCTCTTC 1 cut(s) 2040
SaqAI TTAA 6 cut(s) 273, 489, 633, 1194, 1218, 1424
SatI GCNGC 5 cut(s) 107, 909, 1353, 1383, 1516
ScaI AGTACT 1 cut(s) 388
SchI GAGTC 3 cut(s) 141, 563, 1220
ScrFI CCNGG 3 cut(s) 818, 1255, 1748
SfaNI GCATC 5 cut(s) 331, 1032, 1249, 1372, 1735
SfcI CTRYAG 1 cut(s) 501
SmlI CTYRAG 5 cut(s) 320, 601, 839, 1319, 1373
SmoI CTYRAG 5 cut(s) 320, 601, 839, 1319, 1373
SpeI ACTAGT 1 cut(s) 89
Sse9I AATT 5 cut(s) 5, 18, 692, 1357, 1389
SsiI CCGC 6 cut(s) 107, 240, 401, 997, 1626, 2031
SspI AATATT 1 cut(s) 1468
SspMI CTAG 3 cut(s) 90, 453, 990
StyD4I CCNGG 3 cut(s) 816, 1253, 1746
TaaI ACNGT 7 cut(s) 173, 412, 707, 1192, 1637, 1803, 1920
TaiI ACGT 2 cut(s) 301, 920
TaqI TCGA 2 cut(s) 374, 758
TaqII GACCGA 1 cut(s) 1022
TasI AATT 5 cut(s) 5, 18, 692, 1357, 1389
TatI WGTACW 4 cut(s) 92, 386, 1776, 1912
TauI GCSGC 1 cut(s) 109
Tru1I TTAA 6 cut(s) 273, 489, 633, 1194, 1218, 1424
Tru9I TTAA 6 cut(s) 273, 489, 633, 1194, 1218, 1424
TscAI CASTG 3 cut(s) 76, 712, 991
TseFI GTSAC 4 cut(s) 731, 1439, 1631, 2026
TseI GCWGC 4 cut(s) 908, 1352, 1382, 1515
Tsp45I GTSAC 4 cut(s) 731, 1439, 1631, 2026
TspDTI ATGAA 6 cut(s) 451, 737, 812, 948, 1289, 1802
TspGWI ACGGA 2 cut(s) 242, 1892
TspRI CASTG 3 cut(s) 76, 712, 991
XapI RAATTY 2 cut(s) 5, 1357
XceI RCATGY 2 cut(s) 1783, 2050
XmiI GTMKAC 1 cut(s) 567
XspI CTAG 3 cut(s) 90, 453, 990
ZrmI AGTACT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.