Rh7CG175300

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0006314
Physical Location & Seq
Reverse (-)
14982618 .. 14986813
4196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG175300.1

Sequence Viewer

Length: 2193 bp
ATGGGACTAGAGATGGAGTTCGAGACAAATTGCAGAGTGGGTCTGAGTCCCAACACTGTTCTTCCAACTCATCGGCATTCCTGTGTTGAAAAGAGAAGCACAAAAGGTAGACCGCCACGCAAAGATGATCTATTGAGCATCAAGGAGGGGTTTGTGGAAATCAGCTTTCAGCGCTATCGCAGCACTTCTTGTAAGGTACCATCTAGACCTGTTGGACAGGAAGTTAACATAGAACTTAAGCGAGGTTCTATATATCAAAGCTCCAAGGAGGTTAGGGAAATTAAGAAAATGGGCACTGCTGAGGGAAGGAAAAAGATTGAAAAGTCACGATGTAGTGACTCGTCTTTCTCTTACAGAATTGTCCATTCGTTGCGTAGTTCAGGTGATGAAAGCCCACAGAATAGGTCTTCCGTACTGTCTTTGAAACCCGAACTGAATGTACCATCTGCTGGAAGGCCTCATGTAGAGCCGTGTTCATTGGATAGCATTCTTGAAATTTGTGTGGATCCAGATGACAGGGAAGAGCACTCAGCTGAAGCTTTAGGAGAAGATCCAATGCGTTTGAATCTAAGAAGTGATCCAGTTGCTGGACCTCTAAACAACGGAAATGAACTTCTTGAAAGAGATGAAGTTCACAAATTGCCCAAGTCATTTTCTGCTAAGGTGGAAATGCTTCATACTCCTTCTCCATCGTCGGCGAGTGATCGCTCGTCCAGTGTGAGCTCAAAGGCCCGGTTCAACAATTTAAGAAAAATGTTTGATCCATTCATGAAGTCCAAGTCATTGCGAAGTCCAAGGTATGTTGTGGAACCTGGGGGAGCCAAAACAACTGGAATGGTGGACATGACAAAAAGCAGGACATATCAAAAATCTTTGTTACCTGTCTTTGCAAATACGGTACAGAATGCAGGTTGTGATTCTCTGTGTATCAACCAAGATAACCACCAGTCTAGTGTAGCATCTTCACCGGTTCACCTACATGGCCATCTTAAATTACAAAATAAACATGGAATGCCATTTTTTGAGTTCTTGTTGAAGGGATCAGAAGATGTCTTTGTGGCCAGGACATGGAAAGCGGATAATGGTTTTAACTGGGTGTATACCTTTCATGCCGTTGGTGGTAGAAAGAAGAGCAATGCCAGCGGTGTGGGATTGCATGGTAGCGATAGAGATTCTTCAATGGTGGGACAGATGCCAGTTTCTTGTTATCTATGTTCAGAACTAAGAGATGGAGCTTTTGACAATTCCATGGTGACAGAGTTTGTTTTGTATGATATCTGTCATGCAAGGCAGATTGCAGCAGAAAAAGCACACTCTAATTGTACCCTTGATGATGCTAAACATCCCAAAGGTTCCAATCAGAGCACGGAGGGTGCAACTTTAAAGTTGGGTGATGGGTCTTGTCCAACGAAGGTCAAATCTCAACACAAGAGCAGTGATTCCGATTCTCCAGCCTGGCCATTTGCAGCTCTGCATCCAGGTCTTGAAATTGCAGCCATTGTTATGCAAGTTCCATTTGAAAAGAGAGAGAGCTTGAAATACAAGAGAGGGGATAAGGTATGTGATAAAGTACCTGCAAACCTACTTAAGCTGTCCATGGTTGAACAGAAGAAGAAGGATGACCCTGATAATCGAAACTCAGGAAACTTGAAGTTGATAATTCCATCTGGAAATCATGGTTTTCCGGTTGCTGAAAGCAAGGGTCCTTCGTCGTTACTAGATCGATGGAGGTTGGGTGGTGGTTGTGACTGTGGTGGCTGGGACATGTCCTGTCCTCTTACTGTGTTAAGCAATCCCAACATTCAGTTAGCTGATAATCAATTATTTGAAGAGACTCAGCTGCCTTTGGAACTTTTTGTTCAGGGATCAAAGGAGAAAAAACCAGCATTGACCATGACAATGGTTGAAGATGGACAGTATGCAGTTGATTTTCATGCACAGTTGTCGACATTACAAGCATTCTCTATGTGTGTTTCTATTCTGCATGGTACAGAAACATCGACAGCAGTCACTGGGCAGGAGGAAAGCAAACAGTTGTCGCAGTGCAATTCATTGAAAGTGCTTAAAGAGGAAGTGAAGTTCTTAATTGAAGCAGTCACAGAAGGGGAGAAGAAGAAAGTAGCTAAGAGGGTGAAAAAGATCCAACCATCTTATGTGCTCGATTCACCCTTCTCTCCTATTGCACGAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BAH DUF3527 TFIIS_M

Protein Analysis

730

Amino Acids

80.53

Weight (kDa)

8.6

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 323 - 663 3.5e-106 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 899, 1582
Acc65I GGTACC 1 cut(s) 196
AccB1I GGYRCC 1 cut(s) 196
AccB7I CCANNNNNTGG 3 cut(s) 449, 587, 1068
AccI GTMKAC 3 cut(s) 109, 1100, 1946
AciI CCGC 3 cut(s) 113, 1076, 1143
AclWI GGATC 8 cut(s) 500, 513, 545, 572, 755, 1048, 1873, 2134
AcoI YGGCCR 3 cut(s) 982, 1059, 1457
AcsI RAATTY 1 cut(s) 495
AcuI CTGAAG 1 cut(s) 555
AfaI GTAC 7 cut(s) 198, 414, 441, 900, 1324, 1572, 1990
AfeI AGCGCT 1 cut(s) 173
AfiI CCNNNNNNNGG 3 cut(s) 449, 587, 1068
AflII CTTAAG 2 cut(s) 236, 1586
AflIII ACRYGT 1 cut(s) 1764
AgeI ACCGGT 1 cut(s) 967
AjnI CCWGG 4 cut(s) 811, 1061, 1454, 1477
AloI GAACNNNNNNTCC 2 cut(s) 2063, 2095
Alw21I GWGCWC 4 cut(s) 528, 725, 1367, 2160
Alw26I GTCTC 2 cut(s) 17, 1826
AlwI GGATC 8 cut(s) 500, 513, 545, 572, 755, 1048, 1873, 2134
AlwNI CAGNNNCTG 2 cut(s) 587, 2012
Aor51HI AGCGCT 1 cut(s) 173
AoxI GGCC 5 cut(s) 455, 729, 982, 1059, 1457
ApeKI GCWGC 5 cut(s) 180, 1298, 1466, 1493, 1840
ApoI RAATTY 1 cut(s) 495
AsiGI ACCGGT 1 cut(s) 967
Asp700I GAANNNNTTC 1 cut(s) 672
Asp718I GGTACC 1 cut(s) 196
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 3 cut(s) 590, 730, 1703
AsuC2I CCSGG 1 cut(s) 733
AsuHPI GGTGA 7 cut(s) 395, 957, 965, 1264, 1403, 2143, 2157
AvaII GGWCC 2 cut(s) 590, 1703
BaeGI GKGCMC 1 cut(s) 296
BalI TGGCCA 3 cut(s) 984, 1061, 1459
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 196
BanII GRGCYC 1 cut(s) 725
BauI CACGAG 1 cut(s) 2184
BbsI GAAGAC 1 cut(s) 399
Bbv12I GWGCWC 4 cut(s) 528, 725, 1367, 2160
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 5 cut(s) 192, 1310, 1478, 1505, 1827
BceAI ACGGC 2 cut(s) 454, 1097
BcgI CGANNNNNNTGC 2 cut(s) 1926, 1960
BciT130I CCWGG 4 cut(s) 813, 1063, 1456, 1479
BcnI CCSGG 1 cut(s) 733
BcoDI GTCTC 2 cut(s) 17, 1826
BfaI CTAG 4 cut(s) 8, 204, 951, 1718
BfoI RGCGCY 1 cut(s) 175
BfrI CTTAAG 2 cut(s) 236, 1586
BfuAI ACCTGC 2 cut(s) 899, 1582
BisI GCNGC 5 cut(s) 181, 1299, 1467, 1494, 1841
BlsI GCNGC 5 cut(s) 182, 1300, 1468, 1495, 1842
Bme1390I CCNGG 5 cut(s) 733, 813, 1063, 1456, 1479
Bme18I GGWCC 2 cut(s) 590, 1703
BmgT120I GGNCC 3 cut(s) 590, 730, 1703
BmiI GGNNCC 6 cut(s) 198, 507, 810, 820, 1354, 1704
BmrFI CCNGG 5 cut(s) 733, 813, 1063, 1456, 1479
BmrI ACTGGG 2 cut(s) 1102, 2022
BmsI GCATC 5 cut(s) 147, 968, 1182, 1324, 1483
BmuI ACTGGG 2 cut(s) 1102, 2022
BoxI GACNNNNGTC 1 cut(s) 2006
BpiI GAAGAC 1 cut(s) 399
BpmI CTGGAG 1 cut(s) 1434
Bpu10I CCTNAGC 2 cut(s) 300, 660
BpuMI CCSGG 1 cut(s) 733
Bsa29I ATCGAT 1 cut(s) 1723
BsaJI CCNNGG 5 cut(s) 264, 794, 812, 1248, 1596
BsaWI WCCGGW 2 cut(s) 967, 1684
BsaXI ACNNNNNCTCC 4 cut(s) 670, 700, 1720, 1750
Bsc4I CCNNNNNNNGG 3 cut(s) 449, 587, 1068
Bse118I RCCGGY 1 cut(s) 967
Bse1I ACTGG 7 cut(s) 581, 714, 835, 946, 1097, 1196, 2017
Bse3DI GCAATG 2 cut(s) 782, 1141
BseBI CCWGG 4 cut(s) 813, 1063, 1456, 1479
BseCI ATCGAT 1 cut(s) 1723
BseDI CCNNGG 5 cut(s) 264, 794, 812, 1248, 1596
BseGI GGATG 3 cut(s) 1342, 1474, 1624
BseLI CCNNNNNNNGG 3 cut(s) 449, 587, 1068
BseMI GCAATG 2 cut(s) 782, 1141
BseMII CTCAG 5 cut(s) 35, 291, 543, 1653, 1850
BseNI ACTGG 7 cut(s) 581, 714, 835, 946, 1097, 1196, 2017
BseSI GKGCMC 1 cut(s) 296
BseXI GCAGC 5 cut(s) 192, 1310, 1478, 1505, 1827
BseYI CCCAGC 1 cut(s) 1758
BshFI GGCC 5 cut(s) 457, 731, 984, 1061, 1459
BshNI GGYRCC 1 cut(s) 196
BshTI ACCGGT 1 cut(s) 967
BshVI ATCGAT 1 cut(s) 1723
BsiHKAI GWGCWC 4 cut(s) 528, 725, 1367, 2160
BsiSI CCGG 3 cut(s) 733, 968, 1685
BslFI GGGAC 4 cut(s) 18, 33, 1200, 1775
BslI CCNNNNNNNGG 3 cut(s) 449, 587, 1068
BsmAI GTCTC 2 cut(s) 17, 1826
BsmFI GGGAC 4 cut(s) 18, 33, 1200, 1775
BsmI GAATGC 5 cut(s) 76, 486, 910, 1017, 1958
BsnI GGCC 5 cut(s) 457, 731, 984, 1061, 1459
Bsp1286I GDGCHC 5 cut(s) 296, 528, 725, 1367, 2160
Bsp19I CCATGG 2 cut(s) 1248, 1596
BspACI CCGC 3 cut(s) 113, 1076, 1143
BspANI GGCC 5 cut(s) 457, 731, 984, 1061, 1459
BspCNI CTCAG 5 cut(s) 36, 292, 542, 1652, 1849
BspDI ATCGAT 1 cut(s) 1723
BspHI TCATGA 1 cut(s) 768
BspLI GGNNCC 6 cut(s) 198, 507, 810, 820, 1354, 1704
BspMI ACCTGC 2 cut(s) 899, 1582
BspPI GGATC 8 cut(s) 500, 513, 545, 572, 755, 1048, 1873, 2134
BspQI GCTCTTC 2 cut(s) 516, 1124
BspT107I GGYRCC 1 cut(s) 196
BspTI CTTAAG 2 cut(s) 236, 1586
BsrDI GCAATG 2 cut(s) 782, 1141
BsrFI RCCGGY 1 cut(s) 967
BsrI ACTGG 7 cut(s) 581, 714, 835, 946, 1097, 1196, 2017
BssAI RCCGGY 1 cut(s) 967
BssECI CCNNGG 5 cut(s) 264, 794, 812, 1248, 1596
BssNAI GTATAC 1 cut(s) 1101
BssSI CACGAG 1 cut(s) 2184
BssT1I CCWWGG 4 cut(s) 264, 794, 1248, 1596
Bst1107I GTATAC 1 cut(s) 1101
Bst2BI CACGAG 1 cut(s) 2184
Bst2UI CCWGG 4 cut(s) 813, 1063, 1456, 1479
Bst4CI ACNGT 8 cut(s) 58, 417, 898, 1751, 1783, 1917, 1941, 2034
Bst6I CTCTTC 3 cut(s) 516, 1124, 1824
BstAFI CTTAAG 2 cut(s) 236, 1586
BstC8I GCNNGC 1 cut(s) 1141
BstDEI CTNAG 9 cut(s) 44, 300, 529, 569, 660, 1223, 1639, 1836, 2124
BstDSI CCRYGG 2 cut(s) 1248, 1596
BstF5I GGATG 3 cut(s) 1342, 1474, 1624
BstH2I RGCGCY 1 cut(s) 175
BstHHI GCGC 1 cut(s) 174
BstMAI GTCTC 2 cut(s) 17, 1826
BstMWI GCNNNNNNNGC 4 cut(s) 171, 180, 1140, 1307
BstNI CCWGG 4 cut(s) 813, 1063, 1456, 1479
BstNSI RCATGY 1 cut(s) 1768
BstPAI GACNNNNGTC 1 cut(s) 2006
BstSCI CCNGG 5 cut(s) 731, 811, 1061, 1454, 1477
BstSLI GKGCMC 1 cut(s) 296
BstV1I GCAGC 5 cut(s) 192, 1310, 1478, 1505, 1827
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 3 cut(s) 505, 550, 2139
BstXI CCANNNNNNTGG 2 cut(s) 1147, 1900
BstYI RGATCY 3 cut(s) 505, 550, 2139
BstZ17I GTATAC 1 cut(s) 1101
Bsu15I ATCGAT 1 cut(s) 1723
BsuRI GGCC 5 cut(s) 457, 731, 984, 1061, 1459
BsuTUI ATCGAT 1 cut(s) 1723
BtgI CCRYGG 2 cut(s) 1248, 1596
BtsCI GGATG 3 cut(s) 1342, 1474, 1624
BtsI GCAGTG 3 cut(s) 294, 1441, 2048
BtsIMutI CAGTG 6 cut(s) 54, 294, 721, 1441, 2010, 2048
BveI ACCTGC 2 cut(s) 899, 1582
Cac8I GCNNGC 1 cut(s) 1141
CaiI CAGNNNCTG 2 cut(s) 587, 2012
CciI TCATGA 1 cut(s) 768
CfoI GCGC 1 cut(s) 174
Cfr10I RCCGGY 1 cut(s) 967
Cfr13I GGNCC 3 cut(s) 590, 730, 1703
ClaI ATCGAT 1 cut(s) 1723
Csp6I GTAC 7 cut(s) 197, 413, 440, 899, 1323, 1571, 1989
CspAI ACCGGT 1 cut(s) 967
CviQI GTAC 7 cut(s) 197, 413, 440, 899, 1323, 1571, 1989
DdeI CTNAG 9 cut(s) 44, 300, 529, 569, 660, 1223, 1639, 1836, 2124
DraI TTTAAA 1 cut(s) 1383
EaeI YGGCCR 3 cut(s) 982, 1059, 1457
Eam1104I CTCTTC 3 cut(s) 516, 1124, 1824
EarI CTCTTC 3 cut(s) 516, 1124, 1824
Ecl136II GAGCTC 1 cut(s) 723
Eco130I CCWWGG 4 cut(s) 264, 794, 1248, 1596
Eco147I AGGCCT 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 725
Eco32I GATATC 1 cut(s) 1276
Eco47I GGWCC 2 cut(s) 590, 1703
Eco47III AGCGCT 1 cut(s) 173
Eco53kI GAGCTC 1 cut(s) 723
Eco57I CTGAAG 1 cut(s) 555
EcoICRI GAGCTC 1 cut(s) 723
EcoO109I RGGNCCY 1 cut(s) 1703
EcoRII CCWGG 4 cut(s) 811, 1061, 1454, 1477
EcoRV GATATC 1 cut(s) 1276
EcoT14I CCWWGG 4 cut(s) 264, 794, 1248, 1596
EcoT38I GRGCYC 1 cut(s) 725
ErhI CCWWGG 4 cut(s) 264, 794, 1248, 1596
FaqI GGGAC 4 cut(s) 18, 33, 1200, 1775
FblI GTMKAC 3 cut(s) 109, 1100, 1946
Fnu4HI GCNGC 5 cut(s) 181, 1299, 1467, 1494, 1841
FokI GGATG 3 cut(s) 1329, 1461, 1631
FriOI GRGCYC 1 cut(s) 725
Fsp4HI GCNGC 5 cut(s) 181, 1299, 1467, 1494, 1841
FspBI CTAG 4 cut(s) 8, 204, 951, 1718
GlaI GCGC 1 cut(s) 173
GluI GCNGC 5 cut(s) 181, 1299, 1467, 1494, 1841
GsaI CCCAGC 1 cut(s) 1762
GsuI CTGGAG 1 cut(s) 1434
HaeII RGCGCY 1 cut(s) 175
HaeIII GGCC 5 cut(s) 457, 731, 984, 1061, 1459
HapII CCGG 3 cut(s) 733, 968, 1685
HhaI GCGC 1 cut(s) 174
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HincII GTYRAC 2 cut(s) 226, 1947
HindII GTYRAC 2 cut(s) 226, 1947
HindIII AAGCTT 1 cut(s) 537
HinfI GANTC 9 cut(s) 46, 338, 565, 917, 1172, 1439, 1445, 1834, 2162
HpaI GTTAAC 1 cut(s) 226
HpaII CCGG 3 cut(s) 733, 968, 1685
HphI GGTGA 7 cut(s) 395, 957, 965, 1264, 1403, 2143, 2157
Hpy166II GTNNAC 7 cut(s) 110, 226, 634, 841, 973, 1101, 1947
Hpy188I TCNGA 5 cut(s) 45, 1045, 1219, 1362, 1444
Hpy8I GTNNAC 7 cut(s) 110, 226, 634, 841, 973, 1101, 1947
Hpy99I CGWCG 2 cut(s) 697, 1714
HpyAV CCTTC 9 cut(s) 300, 447, 693, 1030, 1405, 1609, 1716, 2096, 2179
HpyCH4III ACNGT 8 cut(s) 58, 417, 898, 1751, 1783, 1917, 1941, 2034
HpyF10VI GCNNNNNNNGC 4 cut(s) 171, 180, 1140, 1307
HpyF3I CTNAG 9 cut(s) 44, 300, 529, 569, 660, 1223, 1639, 1836, 2124
HspAI GCGC 1 cut(s) 172
KpnI GGTACC 1 cut(s) 200
KspAI GTTAAC 1 cut(s) 226
LguI GCTCTTC 2 cut(s) 516, 1124
LmnI GCTCC 3 cut(s) 266, 818, 1232
Lsp1109I GCAGC 5 cut(s) 192, 1310, 1478, 1505, 1827
LweI GCATC 5 cut(s) 147, 968, 1182, 1324, 1483
MaeI CTAG 4 cut(s) 8, 204, 951, 1718
MaeIII GTNAC 8 cut(s) 324, 335, 876, 1252, 1713, 1745, 2008, 2095
MflI RGATCY 3 cut(s) 505, 550, 2139
MhlI GDGCHC 5 cut(s) 296, 528, 725, 1367, 2160
MlsI TGGCCA 3 cut(s) 984, 1061, 1459
MluNI TGGCCA 3 cut(s) 984, 1061, 1459
MlyI GAGTC 3 cut(s) 55, 332, 1828
MmeI TCCRAC 4 cut(s) 89, 193, 1430, 2167
Mox20I TGGCCA 3 cut(s) 984, 1061, 1459
MroXI GAANNNNTTC 1 cut(s) 672
MscI TGGCCA 3 cut(s) 984, 1061, 1459
MslI CAYNNNNRTG 4 cut(s) 81, 978, 1502, 1898
Msp20I TGGCCA 3 cut(s) 984, 1061, 1459
MspA1I CMGCKG 3 cut(s) 533, 1143, 1840
MspCI CTTAAG 2 cut(s) 236, 1586
MspI CCGG 3 cut(s) 733, 968, 1685
MspR9I CCNGG 5 cut(s) 733, 813, 1063, 1456, 1479
Mva1269I GAATGC 5 cut(s) 76, 486, 910, 1017, 1958
MvaI CCWGG 4 cut(s) 813, 1063, 1456, 1479
MwoI GCNNNNNNNGC 4 cut(s) 171, 180, 1140, 1307
NciI CCSGG 1 cut(s) 733
NcoI CCATGG 2 cut(s) 1248, 1596
NlaIV GGNNCC 6 cut(s) 198, 507, 810, 820, 1354, 1704
NmuCI GTSAC 6 cut(s) 324, 335, 1252, 1745, 2008, 2095
NspI RCATGY 1 cut(s) 1768
PagI TCATGA 1 cut(s) 768
PceI AGGCCT 1 cut(s) 457
PciI ACATGT 1 cut(s) 1764
PciSI GCTCTTC 2 cut(s) 516, 1124
PctI GAATGC 5 cut(s) 76, 486, 910, 1017, 1958
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 6 cut(s) 565, 917, 1172, 1439, 1445, 2162
PflMI CCANNNNNTGG 3 cut(s) 449, 587, 1068
PinAI ACCGGT 1 cut(s) 967
PkrI GCNGC 5 cut(s) 182, 1300, 1468, 1495, 1842
PleI GAGTC 3 cut(s) 54, 332, 1828
PpsI GAGTC 3 cut(s) 54, 332, 1828
PpuMI RGGWCCY 1 cut(s) 1703
PscI ACATGT 1 cut(s) 1764
PshAI GACNNNNGTC 1 cut(s) 2006
Psp124BI GAGCTC 1 cut(s) 725
Psp5II RGGWCCY 1 cut(s) 1703
Psp6I CCWGG 4 cut(s) 811, 1061, 1454, 1477
PspFI CCCAGC 1 cut(s) 1758
PspGI CCWGG 4 cut(s) 811, 1061, 1454, 1477
PspN4I GGNNCC 6 cut(s) 198, 507, 810, 820, 1354, 1704
PspPI GGNCC 3 cut(s) 590, 730, 1703
PspPPI RGGWCCY 1 cut(s) 1703
PsrI GAACNNNNNNTAC 2 cut(s) 423, 455
PstNI CAGNNNCTG 2 cut(s) 587, 2012
PsuI RGATCY 3 cut(s) 505, 550, 2139
PvuII CAGCTG 2 cut(s) 533, 1840
RsaI GTAC 7 cut(s) 198, 414, 441, 900, 1324, 1572, 1990
RsaNI GTAC 7 cut(s) 197, 413, 440, 899, 1323, 1571, 1989
RseI CAYNNNNRTG 4 cut(s) 81, 978, 1502, 1898
SacI GAGCTC 1 cut(s) 725
SalI GTCGAC 1 cut(s) 1945
SapI GCTCTTC 2 cut(s) 516, 1124
SatI GCNGC 5 cut(s) 181, 1299, 1467, 1494, 1841
Sau96I GGNCC 3 cut(s) 590, 730, 1703
SchI GAGTC 3 cut(s) 55, 332, 1828
ScrFI CCNGG 5 cut(s) 733, 813, 1063, 1456, 1479
SduI GDGCHC 5 cut(s) 296, 528, 725, 1367, 2160
SfaNI GCATC 5 cut(s) 147, 968, 1182, 1324, 1483
SinI GGWCC 2 cut(s) 590, 1703
SmiMI CAYNNNNRTG 4 cut(s) 81, 978, 1502, 1898
SmlI CTYRAG 2 cut(s) 236, 1586
SmoI CTYRAG 2 cut(s) 236, 1586
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 3 cut(s) 113, 1076, 1143
SspMI CTAG 4 cut(s) 8, 204, 951, 1718
SstI GAGCTC 1 cut(s) 725
StuI AGGCCT 1 cut(s) 457
StyD4I CCNGG 5 cut(s) 731, 811, 1061, 1454, 1477
StyI CCWWGG 4 cut(s) 264, 794, 1248, 1596
TaaI ACNGT 8 cut(s) 58, 417, 898, 1751, 1783, 1917, 1941, 2034
TaqI TCGA 6 cut(s) 21, 1633, 1723, 1946, 2000, 2160
TfiI GAWTC 6 cut(s) 565, 917, 1172, 1439, 1445, 2162
TscAI CASTG 6 cut(s) 61, 301, 721, 1441, 2017, 2048
TseFI GTSAC 6 cut(s) 324, 335, 1252, 1745, 2008, 2095
TseI GCWGC 5 cut(s) 180, 1298, 1466, 1493, 1840
Tsp45I GTSAC 6 cut(s) 324, 335, 1252, 1745, 2008, 2095
TspGWI ACGGA 3 cut(s) 400, 618, 1382
TspRI CASTG 6 cut(s) 61, 301, 721, 1441, 2017, 2048
Van91I CCANNNNNTGG 3 cut(s) 449, 587, 1068
Vha464I CTTAAG 2 cut(s) 236, 1586
VpaK11BI GGWCC 2 cut(s) 590, 1703
XapI RAATTY 1 cut(s) 495
XbaI TCTAGA 1 cut(s) 203
XceI RCATGY 1 cut(s) 1768
XmiI GTMKAC 3 cut(s) 109, 1100, 1946
XmnI GAANNNNTTC 1 cut(s) 672
XspI CTAG 4 cut(s) 8, 204, 951, 1718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.