MD06G1083000.v1.1

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006314
Physical Location & Seq
Forward (+)
20396428 .. 20400620
4193 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1083000.v1.1.491

Sequence Viewer

Length: 2175 bp
ATGGATTTCGATAGAAATTGCATGGTAGGTTTGAGTCCTAACACTGTGCTTCCAACTCATCGTCCTCATTCCCATGGTGAAAGAAGGAGCACAAAAGGAAAATTAAAACATAAAGATGATTTATTGAGCATAAAGGAGGATTTCGTGGAAATCAGCTTTCATCACTATCGCAGCGCTTCTTGTAAAAGTGTTCCATCTAGGCCTGTTGTTAACATAGACCTTAAGCGTGGTTCTATATATCAAAGCTCCAAAGAGGTACAAAATATTAAGAAAATGGGCACTGTTGAAGGAAGGAGAAAAGTTGGAATGCCGCGAAGTAGTGACACCTCTTTCTCTTACCGAATTGTTGACACGATGTGTAGTTCAGATGAGGAGAGTACACAGAATAGGTCTTCAGTGGTATCTTTGAAATCAGACTTGAACGCACCATCTGTTGGCCGGCCTCATGTTGAACCATGCTCATCAGATAGTTTGATTGATATTTGTATGGATCCCGGTTACAGGGAAAAGCACTCTGCTGAAACTTTACGAGAATTCGCAGTGGATCTGAAACTAAGAAGTGACGCGGTTGCTGGACCTCTAAATAATGGTAATGAACTTCTGGAAAGAGACCAAGTTCACACATTGCACAAGTCTTTCTCTGCTAAGGTGGAAATGCTCCAATCTCGCTCCCCACCAGAGAGTGCTCGCTCATCCAGGGTCAGCTCAAAGACTCGGTTCAGCCCTATTATAAAGATGTTTGATCCATTCATGAAGTCTAAGTCTTTGAGAACTCTGAGTTATGTAGTGGAACCTGGCAAATCCAAAATGTCTGAGCCGGCAAACATGACAAGAAACAGGTCACATAAAAAATCTTTGTTGCCAGTCTATTCAAATACAGCACAGAGTCCAGATTGTGATCCTCAGCGTATCAACCGAGATAGCCACCAATCGGTTGTTGCATGCTCGCCAGTTCACCTACATGGCCATCTTAAGTTACAAAATAAACACGGAATGCCATTTTTTGAGTTCTCGTTGAAGTGCTCAGGAGACGTCTTGGTGGCCAGGACATGGAAAGCAGATAATTCTATTAATTGGGTGTATACCTTTCACTCCATCGGAAGCAGAAAGAAGAGCAATGCCAGCGGTTGGGGATTATATGGTAGTGACAGAGATTCTTCAATGATGGGACAGATGCAAGTGTCCTGTTACCTATGTTCGGAACTCAAAGATGGTGTTTTTGAAAATTCAATGGTGACGGAGTTTGTGGTGTATGATATCGCTCATGCAAGGCAAACGTTTGCAGCCCAAGAAAACTCTAATAGTAGCCTTGATGATGTTAAACCTCCCAAGGGTTCTAGTCCAGGCACAGTTGGGGCAACTGTAAAGTTGGATGAGTCTTGTCCCACCAAGGTCAAACTTCAACAAAAACATGCTTCCGACAAGAGTGATAATGATACTTCAGCCTGGCCGTCTGCAGAATTGCATCCAAGCCTTGAAATTGCAGCCATCGTTATGCAAGTTCCGTTAGAAAAGAGAGAGAGTTTGAAATACAAGAGAGGGGATAAAATTAGTGATAAAGTACATCGAAACCTACTGTCTATGGGTGAACAGAAGGAGGCTGCCCCTGATAGCCAAAGTCCAGGAAACTTACAGGTGGTAGTACCAGCTGGAAACCATGGCACACCGAGTGCTGAAAACAGCGGTCCTTCATCGTTACTAGATCGGTGGAGGTTGGGTGGAGGCTGCGACTGTGGTGGCTGGGACATGTCCTGTCCTCTTATTGTCTTAAGCAATCCCCTCACCGAAAGCTCTGATAATCAACTATTGGCGGAGAATCAGAAGCTTATGGAACTTTTTGTTCAGGGATCGAAGGAGAAAGCACCAGCATTGACCATGACAATGGTTGAAGAGGGACACTATGCAGTTGATTTTCATGCCCGGTTATCTACACTACAAGCATTTTCTACATGTGTTGCCATTCTGCACGGTACAGAAACATCCGCAGCCGCAGGGCAGGAGAGGAACACACAGTTGTCACAATGCAGTTCATTGAAAGTAGTGATTGAGGAAGAAGTGAAGTACTTAATTGAAGCAGTTATGGAAGGGGAGAAGAAGGAAGTAGCTAAGAGGGTGAAAAAAATCCAACCGTCTTATGTACTCAACCCTCCCTTCTCTCCAATTGCTCGGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BAH DUF3527 TFIIS_M

Protein Analysis

725

Amino Acids

80.03

Weight (kDa)

8.59

Isoelectric Point (pI)

54.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 317 - 657 2.2e-112 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 731
AatII GACGTC 1 cut(s) 1035
AccB7I CCANNNNNTGG 3 cut(s) 434, 1050, 1128
AccI GTMKAC 1 cut(s) 1082
AccII CGCG 2 cut(s) 313, 566
AciI CCGC 7 cut(s) 311, 566, 1125, 1683, 1811, 1983, 1989
AclI AACGTT 1 cut(s) 1277
AclWI GGATC 6 cut(s) 485, 498, 552, 737, 893, 1855
AcoI YGGCCR 4 cut(s) 436, 964, 1041, 1448
AcsI RAATTY 2 cut(s) 533, 1225
AcuI CTGAAG 2 cut(s) 378, 1425
AcyI GRCGYC 1 cut(s) 1032
AdeI CACNNNGTG 2 cut(s) 357, 1670
AfaI GTAC 7 cut(s) 258, 379, 1563, 1644, 1972, 2063, 2139
AfeI AGCGCT 1 cut(s) 175
AfiI CCNNNNNNNGG 7 cut(s) 434, 501, 931, 1050, 1128, 1198, 1331
AflII CTTAAG 3 cut(s) 221, 971, 1768
AflIII ACRYGT 2 cut(s) 1746, 1949
AjnI CCWGG 6 cut(s) 695, 793, 1043, 1342, 1445, 1621
AluBI AGCT 7 cut(s) 156, 246, 705, 1649, 1791, 1825, 2105
AluI AGCT 7 cut(s) 156, 246, 705, 1649, 1791, 1825, 2105
Alw21I GWGCWC 3 cut(s) 92, 688, 1025
Alw26I GTCTC 2 cut(s) 603, 1023
AlwI GGATC 6 cut(s) 485, 498, 552, 737, 893, 1855
Ama87I CYCGRG 1 cut(s) 2166
Aor51HI AGCGCT 1 cut(s) 175
AoxI GGCC 6 cut(s) 200, 436, 440, 964, 1041, 1448
ApeKI GCWGC 6 cut(s) 171, 1283, 1484, 1601, 1725, 1985
ApoI RAATTY 2 cut(s) 533, 1225
AseI ATTAAT 1 cut(s) 1071
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 575, 1685
AsuC2I CCSGG 2 cut(s) 495, 1921
AsuHPI GGTGA 6 cut(s) 89, 947, 1246, 1598, 1774, 2125
AvaI CYCGRG 1 cut(s) 2166
AvaII GGWCC 2 cut(s) 575, 1685
BaeGI GKGCMC 1 cut(s) 281
BalI TGGCCA 2 cut(s) 966, 1043
BamHI GGATCC 1 cut(s) 490
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 3 cut(s) 92, 688, 1025
BbvCI CCTCAGC 1 cut(s) 903
BbvI GCAGC 6 cut(s) 183, 1295, 1496, 1588, 1712, 1997
BccI CCATC 7 cut(s) 202, 436, 975, 1103, 1159, 1205, 1496
BceAI ACGGC 1 cut(s) 1435
BciT130I CCWGG 6 cut(s) 697, 795, 1045, 1344, 1447, 1623
BcnI CCSGG 2 cut(s) 495, 1921
BcoDI GTCTC 2 cut(s) 603, 1023
BfaI CTAG 3 cut(s) 198, 1338, 1700
BfmI CTRYAG 1 cut(s) 1455
BfoI RGCGCY 1 cut(s) 177
BfrI CTTAAG 3 cut(s) 221, 971, 1768
BisI GCNGC 8 cut(s) 172, 311, 1284, 1485, 1602, 1726, 1986, 1989
BlsI GCNGC 8 cut(s) 173, 312, 1285, 1486, 1603, 1727, 1987, 1990
BmcAI AGTACT 1 cut(s) 2063
Bme1390I CCNGG 8 cut(s) 495, 697, 795, 1045, 1344, 1447, 1623, 1921
Bme18I GGWCC 2 cut(s) 575, 1685
BmeT110I CYCGRG 1 cut(s) 2166
BmgT120I GGNCC 2 cut(s) 575, 1685
BmiI GGNNCC 2 cut(s) 492, 792
BmrFI CCNGG 8 cut(s) 495, 697, 795, 1045, 1344, 1447, 1623, 1921
BmsI GCATC 2 cut(s) 1164, 1474
BpiI GAAGAC 1 cut(s) 384
Bpu10I CCTNAGC 3 cut(s) 645, 903, 1024
BpuMI CCSGG 2 cut(s) 495, 1921
BsaBI GATNNNNATC 1 cut(s) 897
BsaHI GRCGYC 1 cut(s) 1032
BsaI GGTCTC 1 cut(s) 603
BsaJI CCNNGG 5 cut(s) 73, 696, 1329, 1389, 1657
BsaXI ACNNNNNCTCC 2 cut(s) 1702, 1732
Bsc4I CCNNNNNNNGG 7 cut(s) 434, 501, 931, 1050, 1128, 1198, 1331
Bse118I RCCGGY 2 cut(s) 438, 817
Bse1I ACTGG 2 cut(s) 863, 950
Bse3DI GCAATG 2 cut(s) 623, 1123
Bse8I GATNNNNATC 1 cut(s) 897
BseBI CCWGG 6 cut(s) 697, 795, 1045, 1344, 1447, 1623
BseDI CCNNGG 5 cut(s) 73, 696, 1329, 1389, 1657
BseGI GGATG 4 cut(s) 692, 1378, 1465, 1979
BseJI GATNNNNATC 1 cut(s) 897
BseLI CCNNNNNNNGG 7 cut(s) 434, 501, 931, 1050, 1128, 1198, 1331
BseMI GCAATG 2 cut(s) 623, 1123
BseMII CTCAG 4 cut(s) 767, 804, 917, 1038
BseNI ACTGG 2 cut(s) 863, 950
BseRI GAGGAG 1 cut(s) 386
BseSI GKGCMC 1 cut(s) 281
BseXI GCAGC 6 cut(s) 183, 1295, 1496, 1588, 1712, 1997
BseYI CCCAGC 1 cut(s) 1740
BsgI GTGCAG 1 cut(s) 1949
Bsh1236I CGCG 2 cut(s) 313, 566
BshFI GGCC 6 cut(s) 202, 438, 442, 966, 1043, 1450
BsiHKAI GWGCWC 3 cut(s) 92, 688, 1025
BsiHKCI CYCGRG 1 cut(s) 2166
BsiSI CCGG 4 cut(s) 439, 495, 818, 1921
BslFI GGGAC 4 cut(s) 1182, 1368, 1757, 1908
BslI CCNNNNNNNGG 7 cut(s) 434, 501, 931, 1050, 1128, 1198, 1331
BsmAI GTCTC 2 cut(s) 603, 1023
BsmBI CGTCTC 1 cut(s) 1023
BsmFI GGGAC 4 cut(s) 1182, 1368, 1757, 1908
BsmI GAATGC 2 cut(s) 312, 999
BsnI GGCC 6 cut(s) 202, 438, 442, 966, 1043, 1450
Bso31I GGTCTC 1 cut(s) 603
BsoBI CYCGRG 1 cut(s) 2166
Bsp1286I GDGCHC 4 cut(s) 92, 281, 688, 1025
Bsp143I GATC 6 cut(s) 490, 544, 742, 898, 1702, 1847
Bsp19I CCATGG 2 cut(s) 73, 1657
BspACI CCGC 7 cut(s) 311, 566, 1125, 1683, 1811, 1983, 1989
BspANI GGCC 6 cut(s) 202, 438, 442, 966, 1043, 1450
BspCNI CTCAG 4 cut(s) 768, 805, 916, 1037
BspFNI CGCG 2 cut(s) 313, 566
BspHI TCATGA 1 cut(s) 750
BspLI GGNNCC 2 cut(s) 492, 792
BspMAI CTGCAG 1 cut(s) 1459
BspPI GGATC 6 cut(s) 485, 498, 552, 737, 893, 1855
BspQI GCTCTTC 1 cut(s) 1106
BspTI CTTAAG 3 cut(s) 221, 971, 1768
BspTNI GGTCTC 1 cut(s) 603
BsrDI GCAATG 2 cut(s) 623, 1123
BsrFI RCCGGY 2 cut(s) 438, 817
BsrI ACTGG 2 cut(s) 863, 950
BssAI RCCGGY 2 cut(s) 438, 817
BssECI CCNNGG 5 cut(s) 73, 696, 1329, 1389, 1657
BssMI GATC 6 cut(s) 490, 544, 742, 898, 1702, 1847
BssNAI GTATAC 1 cut(s) 1083
BssNI GRCGYC 1 cut(s) 1032
BssT1I CCWWGG 4 cut(s) 73, 1329, 1389, 1657
Bst1107I GTATAC 1 cut(s) 1083
Bst2UI CCWGG 6 cut(s) 697, 795, 1045, 1344, 1447, 1623
Bst4CI ACNGT 9 cut(s) 46, 283, 1351, 1363, 1578, 1733, 1970, 2013, 2130
Bst6I CTCTTC 2 cut(s) 1106, 1884
BstACI GRCGYC 1 cut(s) 1032
BstAFI CTTAAG 3 cut(s) 221, 971, 1768
BstC8I GCNNGC 6 cut(s) 440, 688, 819, 943, 947, 1123
BstDEI CTNAG 8 cut(s) 554, 645, 759, 776, 813, 903, 1024, 2106
BstDSI CCRYGG 2 cut(s) 73, 1657
BstF5I GGATG 4 cut(s) 692, 1378, 1465, 1979
BstFNI CGCG 2 cut(s) 313, 566
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 6 cut(s) 493, 547, 745, 901, 1705, 1850
BstMAI GTCTC 2 cut(s) 603, 1023
BstMBI GATC 6 cut(s) 490, 544, 742, 898, 1702, 1847
BstMWI GCNNNNNNNGC 1 cut(s) 1122
BstNI CCWGG 6 cut(s) 697, 795, 1045, 1344, 1447, 1623
BstNSI RCATGY 4 cut(s) 945, 1415, 1750, 1953
BstSCI CCNGG 8 cut(s) 493, 695, 793, 1043, 1342, 1445, 1621, 1919
BstSFI CTRYAG 1 cut(s) 1455
BstSLI GKGCMC 1 cut(s) 281
BstUI CGCG 2 cut(s) 313, 566
BstV1I GCAGC 6 cut(s) 183, 1295, 1496, 1588, 1712, 1997
BstV2I GAAGAC 1 cut(s) 384
BstX2I RGATCY 2 cut(s) 490, 544
BstXI CCANNNNNNTGG 1 cut(s) 1882
BstYI RGATCY 2 cut(s) 490, 544
BstZ17I GTATAC 1 cut(s) 1083
BsuRI GGCC 6 cut(s) 202, 438, 442, 966, 1043, 1450
BtgI CCRYGG 2 cut(s) 73, 1657
BtsCI GGATG 4 cut(s) 692, 1378, 1465, 1979
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 4 cut(s) 42, 279, 402, 546
Cac8I GCNNGC 6 cut(s) 440, 688, 819, 943, 947, 1123
CciI TCATGA 1 cut(s) 750
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 2 cut(s) 438, 817
Cfr13I GGNCC 2 cut(s) 575, 1685
CseI GACGC 1 cut(s) 572
Csp6I GTAC 7 cut(s) 257, 378, 1562, 1643, 1971, 2062, 2138
CviQI GTAC 7 cut(s) 257, 378, 1562, 1643, 1971, 2062, 2138
DdeI CTNAG 8 cut(s) 554, 645, 759, 776, 813, 903, 1024, 2106
DpnI GATC 6 cut(s) 492, 546, 744, 900, 1704, 1849
DpnII GATC 6 cut(s) 490, 544, 742, 898, 1702, 1847
DraIII CACNNNGTG 2 cut(s) 357, 1670
EaeI YGGCCR 4 cut(s) 436, 964, 1041, 1448
Eam1104I CTCTTC 2 cut(s) 1106, 1884
EarI CTCTTC 2 cut(s) 1106, 1884
EciI GGCGGA 1 cut(s) 1826
Eco130I CCWWGG 4 cut(s) 73, 1329, 1389, 1657
Eco147I AGGCCT 1 cut(s) 202
Eco31I GGTCTC 1 cut(s) 603
Eco32I GATATC 1 cut(s) 1258
Eco47I GGWCC 2 cut(s) 575, 1685
Eco47III AGCGCT 1 cut(s) 175
Eco57I CTGAAG 2 cut(s) 378, 1425
Eco88I CYCGRG 1 cut(s) 2166
EcoRI GAATTC 1 cut(s) 533
EcoRII CCWGG 6 cut(s) 695, 793, 1043, 1342, 1445, 1621
EcoRV GATATC 1 cut(s) 1258
EcoT14I CCWWGG 4 cut(s) 73, 1329, 1389, 1657
ErhI CCWWGG 4 cut(s) 73, 1329, 1389, 1657
Esp3I CGTCTC 1 cut(s) 1023
FaqI GGGAC 4 cut(s) 1182, 1368, 1757, 1908
FblI GTMKAC 1 cut(s) 1082
Fnu4HI GCNGC 8 cut(s) 172, 311, 1284, 1485, 1602, 1726, 1986, 1989
FokI GGATG 4 cut(s) 679, 1385, 1452, 1966
FseI GGCCGGCC 1 cut(s) 442
Fsp4HI GCNGC 8 cut(s) 172, 311, 1284, 1485, 1602, 1726, 1986, 1989
FspBI CTAG 3 cut(s) 198, 1338, 1700
GlaI GCGC 1 cut(s) 175
GluI GCNGC 8 cut(s) 172, 311, 1284, 1485, 1602, 1726, 1986, 1989
GsaI CCCAGC 1 cut(s) 1744
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 6 cut(s) 202, 438, 442, 966, 1043, 1450
HapII CCGG 4 cut(s) 439, 495, 818, 1921
HgaI GACGC 1 cut(s) 572
HhaI GCGC 1 cut(s) 176
Hin1I GRCGYC 1 cut(s) 1032
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 2 cut(s) 211, 349
HindII GTYRAC 2 cut(s) 211, 349
HindIII AAGCTT 1 cut(s) 1823
HinfI GANTC 6 cut(s) 34, 712, 886, 1154, 1376, 1816
HpaI GTTAAC 1 cut(s) 211
HpaII CCGG 4 cut(s) 439, 495, 818, 1921
HphI GGTGA 6 cut(s) 89, 947, 1246, 1598, 1774, 2125
Hpy166II GTNNAC 7 cut(s) 211, 349, 380, 619, 955, 1083, 1589
Hpy188III TCNNGA 4 cut(s) 602, 751, 890, 1026
Hpy8I GTNNAC 7 cut(s) 211, 349, 380, 619, 955, 1083, 1589
HpyAV CCTTC 9 cut(s) 78, 281, 285, 1588, 1698, 1846, 2078, 2089, 2161
HpyCH4III ACNGT 9 cut(s) 46, 283, 1351, 1363, 1578, 1733, 1970, 2013, 2130
HpyCH4IV ACGT 2 cut(s) 1032, 1277
HpyF10VI GCNNNNNNNGC 1 cut(s) 1122
HpyF3I CTNAG 8 cut(s) 554, 645, 759, 776, 813, 903, 1024, 2106
HpySE526I ACGT 2 cut(s) 1032, 1277
Hsp92I GRCGYC 1 cut(s) 1032
HspAI GCGC 1 cut(s) 174
KroI GCCGGC 2 cut(s) 438, 817
KroNI GCCGGC 2 cut(s) 440, 819
KspAI GTTAAC 1 cut(s) 211
Kzo9I GATC 6 cut(s) 490, 544, 742, 898, 1702, 1847
LguI GCTCTTC 1 cut(s) 1106
LmnI GCTCC 4 cut(s) 87, 251, 663, 674
Lsp1109I GCAGC 6 cut(s) 183, 1295, 1496, 1588, 1712, 1997
LweI GCATC 2 cut(s) 1164, 1474
MaeI CTAG 3 cut(s) 198, 1338, 1700
MaeII ACGT 2 cut(s) 1032, 1277
MalI GATC 6 cut(s) 492, 546, 744, 900, 1704, 1849
MboI GATC 6 cut(s) 490, 544, 742, 898, 1702, 1847
MboII GAAGA 6 cut(s) 384, 1123, 1149, 1901, 2063, 2104
MfeI CAATTG 1 cut(s) 2160
MflI RGATCY 2 cut(s) 490, 544
MhlI GDGCHC 4 cut(s) 92, 281, 688, 1025
MlsI TGGCCA 2 cut(s) 966, 1043
MluNI TGGCCA 2 cut(s) 966, 1043
MlyI GAGTC 4 cut(s) 43, 706, 895, 1385
MmeI TCCRAC 5 cut(s) 77, 283, 1350, 1443, 2149
Mox20I TGGCCA 2 cut(s) 966, 1043
MroNI GCCGGC 2 cut(s) 438, 817
MscI TGGCCA 2 cut(s) 966, 1043
MseI TTAA 9 cut(s) 104, 210, 222, 267, 972, 1071, 1320, 1769, 2066
MslI CAYNNNNRTG 5 cut(s) 72, 114, 960, 1493, 1880
Msp20I TGGCCA 2 cut(s) 966, 1043
MspA1I CMGCKG 3 cut(s) 1125, 1649, 1683
MspCI CTTAAG 3 cut(s) 221, 971, 1768
MspI CCGG 4 cut(s) 439, 495, 818, 1921
MspR9I CCNGG 8 cut(s) 495, 697, 795, 1045, 1344, 1447, 1623, 1921
MunI CAATTG 1 cut(s) 2160
Mva1269I GAATGC 2 cut(s) 312, 999
MvaI CCWGG 6 cut(s) 697, 795, 1045, 1344, 1447, 1623
MvnI CGCG 2 cut(s) 313, 566
MwoI GCNNNNNNNGC 1 cut(s) 1122
NaeI GCCGGC 2 cut(s) 440, 819
NciI CCSGG 2 cut(s) 495, 1921
NcoI CCATGG 2 cut(s) 73, 1657
NdeII GATC 6 cut(s) 490, 544, 742, 898, 1702, 1847
NgoMIV GCCGGC 2 cut(s) 438, 817
NlaIV GGNNCC 2 cut(s) 492, 792
NmuCI GTSAC 6 cut(s) 320, 560, 840, 1145, 1234, 2016
NspI RCATGY 4 cut(s) 945, 1415, 1750, 1953
PaeI GCATGC 1 cut(s) 945
PagI TCATGA 1 cut(s) 750
PceI AGGCCT 1 cut(s) 202
PciI ACATGT 2 cut(s) 1746, 1949
PciSI GCTCTTC 1 cut(s) 1106
PctI GAATGC 2 cut(s) 312, 999
PdiI GCCGGC 2 cut(s) 440, 819
PfeI GAWTC 2 cut(s) 1154, 1816
PflMI CCANNNNNTGG 3 cut(s) 434, 1050, 1128
PfoI TCCNGGA 1 cut(s) 1621
PkrI GCNGC 8 cut(s) 173, 312, 1285, 1486, 1603, 1727, 1987, 1990
PleI GAGTC 4 cut(s) 42, 706, 894, 1384
PpsI GAGTC 4 cut(s) 42, 706, 894, 1384
PscI ACATGT 2 cut(s) 1746, 1949
PshBI ATTAAT 1 cut(s) 1071
PsiI TTATAA 1 cut(s) 731
Psp1406I AACGTT 1 cut(s) 1277
Psp6I CCWGG 6 cut(s) 695, 793, 1043, 1342, 1445, 1621
PspFI CCCAGC 1 cut(s) 1740
PspGI CCWGG 6 cut(s) 695, 793, 1043, 1342, 1445, 1621
PspN4I GGNNCC 2 cut(s) 492, 792
PspPI GGNCC 2 cut(s) 575, 1685
PstI CTGCAG 1 cut(s) 1459
PsuI RGATCY 2 cut(s) 490, 544
PvuII CAGCTG 1 cut(s) 1649
RigI GGCCGGCC 1 cut(s) 442
RsaI GTAC 7 cut(s) 258, 379, 1563, 1644, 1972, 2063, 2139
RsaNI GTAC 7 cut(s) 257, 378, 1562, 1643, 1971, 2062, 2138
RseI CAYNNNNRTG 5 cut(s) 72, 114, 960, 1493, 1880
SapI GCTCTTC 1 cut(s) 1106
SaqAI TTAA 9 cut(s) 104, 210, 222, 267, 972, 1071, 1320, 1769, 2066
SatI GCNGC 8 cut(s) 172, 311, 1284, 1485, 1602, 1726, 1986, 1989
Sau3AI GATC 6 cut(s) 490, 544, 742, 898, 1702, 1847
Sau96I GGNCC 2 cut(s) 575, 1685
ScaI AGTACT 1 cut(s) 2063
SchI GAGTC 4 cut(s) 43, 706, 895, 1385
ScrFI CCNGG 8 cut(s) 495, 697, 795, 1045, 1344, 1447, 1623, 1921
SduI GDGCHC 4 cut(s) 92, 281, 688, 1025
SfaNI GCATC 2 cut(s) 1164, 1474
SfcI CTRYAG 1 cut(s) 1455
SinI GGWCC 2 cut(s) 575, 1685
SmiMI CAYNNNNRTG 5 cut(s) 72, 114, 960, 1493, 1880
SmlI CTYRAG 3 cut(s) 221, 971, 1768
SmoI CTYRAG 3 cut(s) 221, 971, 1768
SphI GCATGC 1 cut(s) 945
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 7 cut(s) 311, 566, 1125, 1683, 1811, 1983, 1989
SspI AATATT 1 cut(s) 265
SspMI CTAG 3 cut(s) 198, 1338, 1700
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 8 cut(s) 493, 695, 793, 1043, 1342, 1445, 1621, 1919
StyI CCWWGG 4 cut(s) 73, 1329, 1389, 1657
TaaI ACNGT 9 cut(s) 46, 283, 1351, 1363, 1578, 1733, 1970, 2013, 2130
TaiI ACGT 2 cut(s) 1035, 1280
TaqI TCGA 3 cut(s) 9, 1567, 1850
TatI WGTACW 4 cut(s) 377, 1561, 2061, 2137
TauI GCSGC 2 cut(s) 313, 1991
TfiI GAWTC 2 cut(s) 1154, 1816
Tru1I TTAA 9 cut(s) 104, 210, 222, 267, 972, 1071, 1320, 1769, 2066
Tru9I TTAA 9 cut(s) 104, 210, 222, 267, 972, 1071, 1320, 1769, 2066
TscAI CASTG 4 cut(s) 49, 286, 402, 546
TseFI GTSAC 6 cut(s) 320, 560, 840, 1145, 1234, 2016
TseI GCWGC 6 cut(s) 171, 1283, 1484, 1601, 1725, 1985
Tsp45I GTSAC 6 cut(s) 320, 560, 840, 1145, 1234, 2016
TspDTI ATGAA 7 cut(s) 149, 609, 739, 767, 1680, 1904, 2019
TspGWI ACGGA 3 cut(s) 1005, 1253, 1494
TspRI CASTG 4 cut(s) 49, 286, 402, 546
Van91I CCANNNNNTGG 3 cut(s) 434, 1050, 1128
Vha464I CTTAAG 3 cut(s) 221, 971, 1768
VpaK11BI GGWCC 2 cut(s) 575, 1685
VspI ATTAAT 1 cut(s) 1071
XapI RAATTY 2 cut(s) 533, 1225
XceI RCATGY 4 cut(s) 945, 1415, 1750, 1953
XmiI GTMKAC 1 cut(s) 1082
XspI CTAG 3 cut(s) 198, 1338, 1700
ZraI GACGTC 1 cut(s) 1033
ZrmI AGTACT 1 cut(s) 2063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.