FvH4_5g02400

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006314
Physical Location & Seq
Forward (+)
1461390 .. 1465720
4331 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g02400.t3

Sequence Viewer

Length: 2190 bp
ATGGGACTAGAAATGGAGTTCGAGAAAAATTGCAGAGTGGGTCTGAGTCCCAACACTGTTCTTCCAACTCATCGGCATTCCTGTGTTGAAAAGAGGAGCACAAAAGGAAGGCCGCCACGCAGAGATGATCTATTGAGTATCAAGGAGGGGTTTGTGGAAATCAGCTTTCAACGCTATCGCAGCACTTCTTGTAAGGTACCATCTAGACCTGTTGGACAGGAAGTTAACGTTGAACTTAAGCGAGGTTCTATATATCAAAGCTCCAAGGAGGTTAGGGAAATTAAGAAAATGGGCACTGCTGAGGGAAGGAAAAAGATTGAAAAGTCTCGAAGTAGTGACTCCTCTTTCTCTTACAGAATTATTGATACGTTACATAGTTCAGGTGATGAAAGTCCACAGAATAGGTCTTCAGTACTGTCTTTGAAACCCGAATTGAATGTACCATCTGCTGGAAGGCCTCATGTAGAACCGTGTTCATTGGATAGCATTCTAGAAATTTGTGTGGATCCAGATGACAGGGAAGAGCACTCAGCTGAAGCTTTAGGAGAAGATCCAATGCATTTGAATCTAAGAAGTGATCCGGTTGCTGGACCACTAAACAACGGAAATGAACTTCTTGAAAGGGATGAAGTTCACAAATTGCACAAGTCATTCTCTGCTAAGGTGGAAATGCTTCATACTCCTTCTCCATCAGAGAGTGATCGCTCGTCCAGTGTGAGCTCAAAGGCCCGGTTCAATAATATAAGAAAGATGTTTGATCCATTTAAGAAGTCCAAGTCATTGCGAAGTCCAAATTATGTTGTGGAGCCTGGGGGAGCCAAAACAACTGGGAAGGAAGACATGACAAAGGGCAGGACTCATCAAAAATCTTTGTTGCCTGTCTTTTCAAATACGGTACAGAATGCAGGTGGTGATTCTCTGTGTGTCAACCAAGATAACCACCAGTCTAGCGTAGCGTCTTCACCGGTTCACCTACATGGCCGTCTTAAGTTACAAAATAAACATGGAATGCCTTTATTTGAGTTCTTGTTGAAGGGATCAGAAGATGTCTTCGTGGCCAGGACATGGAAAGCAGACAATGGTTTTAACTGGGTGTATACCTTTCATGCCATTGGCGGTAGAAAGAAGAGCAATGCCAGCGGTGTGGGATCACATGGTAGCGATAGAGATTCTTCAATGGTGGGACAAATGCAAGTTTCTTGTTATCTATGTTCAGAACTGAAGGATGGAGCTTTTGACAATTCCATGTCGACAGAGTTTGTTTTGTACGATATTTCTCATGCAAGGCAAATTGCAGCAGAAAAAGAAAACTCTAATTGTACCCTTGATGATGCTAAACATCCCAAAGGTTCCAATCAGAGCACAGAGGGTGCAACTTTAAAGTTGGGTGATGGGTCTTGTCCAACGAAGGTCAAACATCAACACAAGAGTAGTGATACCGATTCTCCAGCCTGGCCATTTGCAGCTTTGCATCCAGGTCTTGAAATTGTAGCCATTGTTATGCAAGTTCCATTTGAAAAGAGAGAGAGCTTGAAATACAAGAGAGGGGACAAGGTATGTGATAAAGTACCTGCAAACCTACTCAAGCTCCCTATGGTCAAACAGAAGAAGAAGAATGACCCTGATAGTCGAAACTCAGGAAACCTGAAGGTGATAATTCCATCTGGAAACCATGGTTTGCCGAGTACTGAAAGCAAGGGTCCTTCATCATTACTAGATCGATGGAGATTTGGTGGTGGTTGTGACTGTGGTGGCTGGGACATGTCTTGTCCTCTTACTGTGTTAAGCAATCCCAACATTCAGTTGGCTGATAATCAATTATTTGAAGAGAATCAGCAGCCTTTGGAACTTTTTGTTCAGGGATCAAAGGAGAAACAACCAGCATTGACCATGACAATGGTTGAAGATGGACAGTATGCAGTTGATTTTCATGCACAGTTATCAACGTTACAAGCATTCTCTATGTGTGTTTCCATTCTGCATGGTACAGAAACATCGGCAGCTGCCACTGGGCTGGAGGAAAACACACAGTTGTCACAGTGCAGTTCATTGAAAGTGCTTAAAGAGGAAGTGAAGTTCTTAATTGAAGCAGTCACAGAAGGGGAGAAGAAGAAAGTAGCCAAGAGGGTGAAAAAGATCCAACCATGTTATACACTCGATCCACCTTTCTCTCCTATTGCACGAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BAH DUF3527 TFIIS_M

Protein Analysis

730

Amino Acids

80.43

Weight (kDa)

8.48

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 322 - 662 3.4e-109 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 896
Acc36I ACCTGC 2 cut(s) 896, 1579
Acc65I GGTACC 1 cut(s) 196
AccB1I GGYRCC 1 cut(s) 196
AccB7I CCANNNNNTGG 2 cut(s) 449, 1065
AccI GTMKAC 2 cut(s) 1097, 1250
AciI CCGC 3 cut(s) 113, 1116, 1140
AclI AACGTT 2 cut(s) 228, 1946
AcoI YGGCCR 3 cut(s) 979, 1056, 1454
AcsI RAATTY 1 cut(s) 495
AcuI CTGAAG 4 cut(s) 393, 555, 1241, 1667
AfaI GTAC 9 cut(s) 198, 414, 441, 897, 1268, 1321, 1569, 1687, 1987
AfiI CCNNNNNNNGG 3 cut(s) 449, 587, 1065
AflII CTTAAG 2 cut(s) 236, 986
AflIII ACRYGT 1 cut(s) 1761
AgeI ACCGGT 1 cut(s) 964
AjnI CCWGG 4 cut(s) 808, 1058, 1451, 1474
AloI GAACNNNNNNTCC 2 cut(s) 2060, 2092
Alw21I GWGCWC 4 cut(s) 101, 528, 722, 1364
Alw26I GTCTC 1 cut(s) 330
AoxI GGCC 6 cut(s) 110, 455, 726, 979, 1056, 1454
ApeKI GCWGC 6 cut(s) 180, 1295, 1463, 1837, 2000, 2003
ApoI RAATTY 1 cut(s) 495
AsiGI ACCGGT 1 cut(s) 964
Asp700I GAANNNNTTC 1 cut(s) 672
Asp718I GGTACC 1 cut(s) 196
AspS9I GGNCC 3 cut(s) 590, 727, 1700
AsuC2I CCSGG 1 cut(s) 730
AsuHPI GGTGA 7 cut(s) 395, 923, 954, 962, 1400, 1663, 2140
AvaII GGWCC 2 cut(s) 590, 1700
BaeGI GKGCMC 1 cut(s) 296
BalI TGGCCA 2 cut(s) 1058, 1456
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 196
BanII GRGCYC 1 cut(s) 722
BauI CACGAG 1 cut(s) 2181
BbsI GAAGAC 4 cut(s) 399, 843, 951, 1042
Bbv12I GWGCWC 4 cut(s) 101, 528, 722, 1364
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 6 cut(s) 192, 1307, 1475, 1849, 1990, 2012
BccI CCATC 8 cut(s) 208, 451, 697, 1220, 1385, 1669, 1716, 1901
BceAI ACGGC 1 cut(s) 966
BciT130I CCWGG 4 cut(s) 810, 1060, 1453, 1476
BcnI CCSGG 1 cut(s) 730
BcoDI GTCTC 1 cut(s) 330
BfaI CTAG 5 cut(s) 8, 204, 491, 948, 1715
BfrI CTTAAG 2 cut(s) 236, 986
BfuAI ACCTGC 2 cut(s) 896, 1579
BisI GCNGC 7 cut(s) 113, 181, 1296, 1464, 1838, 2001, 2004
BlsI GCNGC 7 cut(s) 114, 182, 1297, 1465, 1839, 2002, 2005
BmcAI AGTACT 2 cut(s) 414, 1687
Bme1390I CCNGG 5 cut(s) 730, 810, 1060, 1453, 1476
Bme18I GGWCC 2 cut(s) 590, 1700
BmgT120I GGNCC 3 cut(s) 590, 727, 1700
BmiI GGNNCC 6 cut(s) 198, 507, 807, 817, 1351, 1701
BmrFI CCNGG 5 cut(s) 730, 810, 1060, 1453, 1476
BmrI ACTGGG 3 cut(s) 837, 1099, 2019
BmsI GCATC 2 cut(s) 1321, 1480
BmuI ACTGGG 3 cut(s) 837, 1099, 2019
BpiI GAAGAC 4 cut(s) 399, 843, 951, 1042
BpmI CTGGAG 2 cut(s) 1431, 2036
Bpu10I CCTNAGC 2 cut(s) 300, 660
BpuEI CTTGAG 1 cut(s) 1568
BpuMI CCSGG 1 cut(s) 730
Bsa29I ATCGAT 1 cut(s) 1720
BsaJI CCNNGG 3 cut(s) 264, 809, 1672
BsaWI WCCGGW 2 cut(s) 580, 964
BsaXI ACNNNNNCTCC 8 cut(s) 670, 700, 1429, 1459, 1572, 1602, 1717, 1747
Bsc4I CCNNNNNNNGG 3 cut(s) 449, 587, 1065
Bse118I RCCGGY 1 cut(s) 964
Bse1I ACTGG 5 cut(s) 711, 832, 943, 1094, 2014
Bse3DI GCAATG 2 cut(s) 779, 1138
BseBI CCWGG 4 cut(s) 810, 1060, 1453, 1476
BseCI ATCGAT 1 cut(s) 1720
BseDI CCNNGG 3 cut(s) 264, 809, 1672
BseGI GGATG 4 cut(s) 631, 1231, 1339, 1471
BseLI CCNNNNNNNGG 3 cut(s) 449, 587, 1065
BseMI GCAATG 2 cut(s) 779, 1138
BseMII CTCAG 4 cut(s) 35, 291, 543, 1650
BseNI ACTGG 5 cut(s) 711, 832, 943, 1094, 2014
BseRI GAGGAG 2 cut(s) 109, 331
BseSI GKGCMC 1 cut(s) 296
BseXI GCAGC 6 cut(s) 192, 1307, 1475, 1849, 1990, 2012
BseYI CCCAGC 1 cut(s) 1755
BsgI GTGCAG 1 cut(s) 2062
BshFI GGCC 6 cut(s) 112, 457, 728, 981, 1058, 1456
BshNI GGYRCC 1 cut(s) 196
BshTI ACCGGT 1 cut(s) 964
BshVI ATCGAT 1 cut(s) 1720
BsiHKAI GWGCWC 4 cut(s) 101, 528, 722, 1364
BsiSI CCGG 3 cut(s) 581, 730, 965
BslFI GGGAC 5 cut(s) 18, 33, 1197, 1562, 1772
BslI CCNNNNNNNGG 3 cut(s) 449, 587, 1065
BsmAI GTCTC 1 cut(s) 330
BsmFI GGGAC 5 cut(s) 18, 33, 1197, 1562, 1772
BsmI GAATGC 5 cut(s) 76, 486, 907, 1014, 1955
BsnI GGCC 6 cut(s) 112, 457, 728, 981, 1058, 1456
Bsp1286I GDGCHC 5 cut(s) 101, 296, 528, 722, 1364
Bsp19I CCATGG 1 cut(s) 1672
BspACI CCGC 3 cut(s) 113, 1116, 1140
BspANI GGCC 6 cut(s) 112, 457, 728, 981, 1058, 1456
BspCNI CTCAG 4 cut(s) 36, 292, 542, 1649
BspDI ATCGAT 1 cut(s) 1720
BspLI GGNNCC 6 cut(s) 198, 507, 807, 817, 1351, 1701
BspMI ACCTGC 2 cut(s) 896, 1579
BspQI GCTCTTC 2 cut(s) 516, 1121
BspT107I GGYRCC 1 cut(s) 196
BspTI CTTAAG 2 cut(s) 236, 986
BsrDI GCAATG 2 cut(s) 779, 1138
BsrFI RCCGGY 1 cut(s) 964
BsrI ACTGG 5 cut(s) 711, 832, 943, 1094, 2014
BssAI RCCGGY 1 cut(s) 964
BssECI CCNNGG 3 cut(s) 264, 809, 1672
BssNAI GTATAC 1 cut(s) 1098
BssSI CACGAG 1 cut(s) 2181
BssT1I CCWWGG 2 cut(s) 264, 1672
Bst1107I GTATAC 1 cut(s) 1098
Bst2BI CACGAG 1 cut(s) 2181
Bst2UI CCWGG 4 cut(s) 810, 1060, 1453, 1476
Bst6I CTCTTC 3 cut(s) 516, 1121, 1821
BstAFI CTTAAG 2 cut(s) 236, 986
BstC8I GCNNGC 1 cut(s) 1138
BstDEI CTNAG 6 cut(s) 44, 300, 529, 569, 660, 1636
BstDSI CCRYGG 1 cut(s) 1672
BstF5I GGATG 4 cut(s) 631, 1231, 1339, 1471
BstMAI GTCTC 1 cut(s) 330
BstMWI GCNNNNNNNGC 3 cut(s) 171, 180, 1137
BstNI CCWGG 4 cut(s) 810, 1060, 1453, 1476
BstNSI RCATGY 1 cut(s) 1765
BstSCI CCNGG 5 cut(s) 728, 808, 1058, 1451, 1474
BstSLI GKGCMC 1 cut(s) 296
BstV1I GCAGC 6 cut(s) 192, 1307, 1475, 1849, 1990, 2012
BstV2I GAAGAC 4 cut(s) 399, 843, 951, 1042
BstX2I RGATCY 3 cut(s) 505, 550, 2136
BstXI CCANNNNNNTGG 3 cut(s) 1144, 1897, 2014
BstYI RGATCY 3 cut(s) 505, 550, 2136
BstZ17I GTATAC 1 cut(s) 1098
Bsu15I ATCGAT 1 cut(s) 1720
BsuRI GGCC 6 cut(s) 112, 457, 728, 981, 1058, 1456
BsuTUI ATCGAT 1 cut(s) 1720
BtgI CCRYGG 1 cut(s) 1672
BtsCI GGATG 4 cut(s) 631, 1231, 1339, 1471
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 5 cut(s) 54, 294, 718, 2007, 2045
BveI ACCTGC 2 cut(s) 896, 1579
Cac8I GCNNGC 1 cut(s) 1138
Cfr10I RCCGGY 1 cut(s) 964
Cfr13I GGNCC 3 cut(s) 590, 727, 1700
ClaI ATCGAT 1 cut(s) 1720
CseI GACGC 1 cut(s) 945
Csp6I GTAC 9 cut(s) 197, 413, 440, 896, 1267, 1320, 1568, 1686, 1986
CspAI ACCGGT 1 cut(s) 964
CviQI GTAC 9 cut(s) 197, 413, 440, 896, 1267, 1320, 1568, 1686, 1986
DdeI CTNAG 6 cut(s) 44, 300, 529, 569, 660, 1636
DraI TTTAAA 1 cut(s) 1380
EaeI YGGCCR 3 cut(s) 979, 1056, 1454
Eam1104I CTCTTC 3 cut(s) 516, 1121, 1821
EarI CTCTTC 3 cut(s) 516, 1121, 1821
Ecl136II GAGCTC 1 cut(s) 720
Eco130I CCWWGG 2 cut(s) 264, 1672
Eco147I AGGCCT 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 722
Eco47I GGWCC 2 cut(s) 590, 1700
Eco53kI GAGCTC 1 cut(s) 720
Eco57I CTGAAG 4 cut(s) 393, 555, 1241, 1667
EcoICRI GAGCTC 1 cut(s) 720
EcoO109I RGGNCCY 1 cut(s) 1700
EcoRII CCWGG 4 cut(s) 808, 1058, 1451, 1474
EcoT14I CCWWGG 2 cut(s) 264, 1672
EcoT22I ATGCAT 1 cut(s) 561
EcoT38I GRGCYC 1 cut(s) 722
ErhI CCWWGG 2 cut(s) 264, 1672
FaqI GGGAC 5 cut(s) 18, 33, 1197, 1562, 1772
FblI GTMKAC 2 cut(s) 1097, 1250
Fnu4HI GCNGC 7 cut(s) 113, 181, 1296, 1464, 1838, 2001, 2004
FokI GGATG 4 cut(s) 638, 1238, 1326, 1458
FriOI GRGCYC 1 cut(s) 722
Fsp4HI GCNGC 7 cut(s) 113, 181, 1296, 1464, 1838, 2001, 2004
FspBI CTAG 5 cut(s) 8, 204, 491, 948, 1715
GluI GCNGC 7 cut(s) 113, 181, 1296, 1464, 1838, 2001, 2004
GsaI CCCAGC 1 cut(s) 1759
GsuI CTGGAG 2 cut(s) 1431, 2036
HaeIII GGCC 6 cut(s) 112, 457, 728, 981, 1058, 1456
HapII CCGG 3 cut(s) 581, 730, 965
HgaI GACGC 1 cut(s) 945
HincII GTYRAC 3 cut(s) 226, 928, 1251
HindII GTYRAC 3 cut(s) 226, 928, 1251
HindIII AAGCTT 1 cut(s) 537
HinfI GANTC 8 cut(s) 46, 338, 565, 856, 914, 1169, 1442, 1831
HpaI GTTAAC 1 cut(s) 226
HpaII CCGG 3 cut(s) 581, 730, 965
HphI GGTGA 7 cut(s) 395, 923, 954, 962, 1400, 1663, 2140
Hpy166II GTNNAC 7 cut(s) 226, 395, 634, 928, 970, 1098, 1251
Hpy188I TCNGA 5 cut(s) 45, 694, 1042, 1216, 1359
Hpy188III TCNNGA 9 cut(s) 22, 204, 327, 491, 509, 617, 1481, 1638, 1665
Hpy8I GTNNAC 7 cut(s) 226, 395, 634, 928, 970, 1098, 1251
HpyCH4IV ACGT 3 cut(s) 228, 368, 1946
HpyF10VI GCNNNNNNNGC 3 cut(s) 171, 180, 1137
HpyF3I CTNAG 6 cut(s) 44, 300, 529, 569, 660, 1636
HpySE526I ACGT 3 cut(s) 228, 368, 1946
KpnI GGTACC 1 cut(s) 200
KspAI GTTAAC 1 cut(s) 226
LguI GCTCTTC 2 cut(s) 516, 1121
LmnI GCTCC 6 cut(s) 96, 266, 805, 815, 1229, 1593
Lsp1109I GCAGC 6 cut(s) 192, 1307, 1475, 1849, 1990, 2012
LweI GCATC 2 cut(s) 1321, 1480
MaeI CTAG 5 cut(s) 8, 204, 491, 948, 1715
MaeII ACGT 3 cut(s) 228, 368, 1946
MaeIII GTNAC 7 cut(s) 335, 369, 990, 1742, 1947, 2034, 2092
MflI RGATCY 3 cut(s) 505, 550, 2136
MhlI GDGCHC 5 cut(s) 101, 296, 528, 722, 1364
MlsI TGGCCA 2 cut(s) 1058, 1456
MluNI TGGCCA 2 cut(s) 1058, 1456
MlyI GAGTC 3 cut(s) 55, 332, 850
MmeI TCCRAC 4 cut(s) 89, 193, 1427, 2164
Mox20I TGGCCA 2 cut(s) 1058, 1456
Mph1103I ATGCAT 1 cut(s) 561
MroXI GAANNNNTTC 1 cut(s) 672
MscI TGGCCA 2 cut(s) 1058, 1456
MslI CAYNNNNRTG 4 cut(s) 81, 975, 1499, 1895
Msp20I TGGCCA 2 cut(s) 1058, 1456
MspA1I CMGCKG 3 cut(s) 533, 1140, 2003
MspCI CTTAAG 2 cut(s) 236, 986
MspI CCGG 3 cut(s) 581, 730, 965
MspR9I CCNGG 5 cut(s) 730, 810, 1060, 1453, 1476
Mva1269I GAATGC 5 cut(s) 76, 486, 907, 1014, 1955
MvaI CCWGG 4 cut(s) 810, 1060, 1453, 1476
MwoI GCNNNNNNNGC 3 cut(s) 171, 180, 1137
NciI CCSGG 1 cut(s) 730
NcoI CCATGG 1 cut(s) 1672
NlaIV GGNNCC 6 cut(s) 198, 507, 807, 817, 1351, 1701
NmeAIII GCCGAG 1 cut(s) 1707
NmuCI GTSAC 4 cut(s) 335, 1742, 2034, 2092
NsiI ATGCAT 1 cut(s) 561
NspI RCATGY 1 cut(s) 1765
PaqCI CACCTGC 1 cut(s) 896
PceI AGGCCT 1 cut(s) 457
PciI ACATGT 1 cut(s) 1761
PciSI GCTCTTC 2 cut(s) 516, 1121
PctI GAATGC 5 cut(s) 76, 486, 907, 1014, 1955
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 5 cut(s) 565, 914, 1169, 1442, 1831
PflMI CCANNNNNTGG 2 cut(s) 449, 1065
PinAI ACCGGT 1 cut(s) 964
PkrI GCNGC 7 cut(s) 114, 182, 1297, 1465, 1839, 2002, 2005
PleI GAGTC 3 cut(s) 54, 332, 850
PpsI GAGTC 3 cut(s) 54, 332, 850
PpuMI RGGWCCY 1 cut(s) 1700
PscI ACATGT 1 cut(s) 1761
Psp124BI GAGCTC 1 cut(s) 722
Psp1406I AACGTT 2 cut(s) 228, 1946
Psp5II RGGWCCY 1 cut(s) 1700
Psp6I CCWGG 4 cut(s) 808, 1058, 1451, 1474
PspFI CCCAGC 1 cut(s) 1755
PspGI CCWGG 4 cut(s) 808, 1058, 1451, 1474
PspN4I GGNNCC 6 cut(s) 198, 507, 807, 817, 1351, 1701
PspPI GGNCC 3 cut(s) 590, 727, 1700
PspPPI RGGWCCY 1 cut(s) 1700
PsuI RGATCY 3 cut(s) 505, 550, 2136
PvuII CAGCTG 2 cut(s) 533, 2003
RsaI GTAC 9 cut(s) 198, 414, 441, 897, 1268, 1321, 1569, 1687, 1987
RsaNI GTAC 9 cut(s) 197, 413, 440, 896, 1267, 1320, 1568, 1686, 1986
RseI CAYNNNNRTG 4 cut(s) 81, 975, 1499, 1895
SacI GAGCTC 1 cut(s) 722
SalI GTCGAC 1 cut(s) 1249
SapI GCTCTTC 2 cut(s) 516, 1121
SatI GCNGC 7 cut(s) 113, 181, 1296, 1464, 1838, 2001, 2004
Sau96I GGNCC 3 cut(s) 590, 727, 1700
ScaI AGTACT 2 cut(s) 414, 1687
SchI GAGTC 3 cut(s) 55, 332, 850
ScrFI CCNGG 5 cut(s) 730, 810, 1060, 1453, 1476
SduI GDGCHC 5 cut(s) 101, 296, 528, 722, 1364
SfaNI GCATC 2 cut(s) 1321, 1480
SinI GGWCC 2 cut(s) 590, 1700
SmiMI CAYNNNNRTG 4 cut(s) 81, 975, 1499, 1895
SmlI CTYRAG 3 cut(s) 236, 986, 1583
SmoI CTYRAG 3 cut(s) 236, 986, 1583
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 3 cut(s) 113, 1116, 1140
SspMI CTAG 5 cut(s) 8, 204, 491, 948, 1715
SstI GAGCTC 1 cut(s) 722
StuI AGGCCT 1 cut(s) 457
StyD4I CCNGG 5 cut(s) 728, 808, 1058, 1451, 1474
StyI CCWWGG 2 cut(s) 264, 1672
TaiI ACGT 3 cut(s) 231, 371, 1949
TaqI TCGA 6 cut(s) 21, 328, 1250, 1630, 1720, 2157
TatI WGTACW 2 cut(s) 412, 1685
TauI GCSGC 1 cut(s) 115
TfiI GAWTC 5 cut(s) 565, 914, 1169, 1442, 1831
TscAI CASTG 5 cut(s) 61, 301, 718, 2014, 2045
TseFI GTSAC 4 cut(s) 335, 1742, 2034, 2092
TseI GCWGC 6 cut(s) 180, 1295, 1463, 1837, 2000, 2003
Tsp45I GTSAC 4 cut(s) 335, 1742, 2034, 2092
TspDTI ATGAA 9 cut(s) 402, 465, 624, 642, 665, 1094, 1695, 1919, 2037
TspGWI ACGGA 1 cut(s) 618
TspRI CASTG 5 cut(s) 61, 301, 718, 2014, 2045
Van91I CCANNNNNTGG 2 cut(s) 449, 1065
Vha464I CTTAAG 2 cut(s) 236, 986
VpaK11BI GGWCC 2 cut(s) 590, 1700
XapI RAATTY 1 cut(s) 495
XbaI TCTAGA 2 cut(s) 203, 490
XceI RCATGY 1 cut(s) 1765
XcmI CCANNNNNNNNNTGG 1 cut(s) 1801
XmiI GTMKAC 2 cut(s) 1097, 1250
XmnI GAANNNNTTC 1 cut(s) 672
XspI CTAG 5 cut(s) 8, 204, 491, 948, 1715
ZrmI AGTACT 2 cut(s) 414, 1687
Zsp2I ATGCAT 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.