Rorug07G0042500

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0006314
Physical Location & Seq
Forward (+)
2985283 .. 2987248
1966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0042500.1

Sequence Viewer

Length: 1065 bp
ATGATGCAAGAAATGGAGAAGTTTGCTGTTCAAGCTGGAACTTTCAAGGAAGATGATCAGATTGATTTGCCACCGGGGTTTCGATTCCATCCAACCGATGAAGAGCTTATTTCTCACTACCTGCACAAGAAGGTTATCGATTCCAACTTCGGTTGCAAAGCAATTGGTGATGTGGACTTGAACAAGTCTGAGCCTTGGGATTTGCCATATAAGGCGAAAATGGGAGAGAAGGAATGGTACTTTTTCTGTGTGAGGGATAGAAAGTATCCGACTGGTCTGAGGACAAACAGAGCAACTGAAGCTGGATATTGGAAAGCTACTGGGAAAGACAAGGAGATTTACAAAGGGAAATCCTTGGTTGGCATGAAGAAGACCCTTGTTTTCTACAGGGGCAGAGCCCCAAAAGGTGAAAAGAGCAACTGGGTCATGCATGAATACAGACTGGAGGGTAAATTCTCAGTCCACAACCTCCCCAAATCTGCAAAGAACGAATGGGTGATTTGTAGGGTCTTTGAAAAATCTGCTGGTGGTAAAAAAATCCATATTACTGGACTTGTGAATTCGGGTCCTCATGGAACTGAAATGGGTTCTTCTGGTTTACCACCTTTGTTGGACTCGGCGCCTTACAATGGAGCCAAGACCAGACCTGCTTCAGAGTCGAATTACGTGCCCTGCTTCTCCAACCATGAGTCCATTGATTCTCAAAGAAATCAAGGGATTGTTGATTACATCAACAGCAATCCTCTTTTCTCTGTTTCTTCGAATCCTTCAAACAACAACAACGCTTTCCCACGAGTCACGTATCAGAATTCACTCTGCTCTGCTCCCGTTTCATCTGCGAATTTCCAGTTTCCGAGTTCGGTTTTAATGCAAGACCATCTGAGGGCCTTGCTTGAAAACAATGTCTCATTCATGAGGCAGAACTTCAAAACAGAGAGGGACATGATCAGTGTGTCTCAAGAAACAGGTCTTACTACTGATGTGAACCCTGAAATCTCTTCTGTCATGCCAAATCCTGAGATGGGTAGGAGGCCATTTGATGATCAAGATAACTTTTGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BAH DUF3527 TFIIS_M

Protein Analysis

354

Amino Acids

40.12

Weight (kDa)

8.14

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 24 - 148 8.9e-50 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 129, 655
AccB1I GGYRCC 1 cut(s) 619
AcsI RAATTY 4 cut(s) 452, 559, 808, 841
AcuI CTGAAG 2 cut(s) 318, 636
AcyI GRCGYC 1 cut(s) 620
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 3 cut(s) 629, 883, 1022
AgsI TTSAA 7 cut(s) 32, 46, 181, 515, 771, 896, 928
AluBI AGCT 4 cut(s) 35, 106, 302, 317
AluI AGCT 4 cut(s) 35, 106, 302, 317
Alw26I GTCTC 2 cut(s) 910, 960
AoxI GGCC 2 cut(s) 885, 1031
ApoI RAATTY 4 cut(s) 452, 559, 808, 841
AspLEI GCGC 1 cut(s) 622
AspS9I GGNCC 2 cut(s) 566, 885
AsuC2I CCSGG 1 cut(s) 75
AsuHPI GGTGA 3 cut(s) 179, 419, 508
AsuII TTCGAA 1 cut(s) 761
AvaII GGWCC 1 cut(s) 566
BaeGI GKGCMC 1 cut(s) 672
BanI GGYRCC 1 cut(s) 619
BanII GRGCYC 1 cut(s) 400
BarI GAAGNNNNNNTAC 2 cut(s) 221, 253
BauI CACGAG 1 cut(s) 792
BbsI GAAGAC 1 cut(s) 377
BccI CCATC 3 cut(s) 96, 885, 1015
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BciVI GTATCC 1 cut(s) 276
BclI TGATCA 3 cut(s) 55, 945, 1042
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 2 cut(s) 910, 960
BfmI CTRYAG 1 cut(s) 385
BfoI RGCGCY 1 cut(s) 623
BfuAI ACCTGC 2 cut(s) 129, 655
BfuI GTATCC 1 cut(s) 276
Bme1390I CCNGG 1 cut(s) 75
Bme18I GGWCC 1 cut(s) 566
BmgT120I GGNCC 2 cut(s) 566, 885
BmiI GGNNCC 3 cut(s) 567, 621, 634
BmrFI CCNGG 1 cut(s) 75
BmrI ACTGGG 2 cut(s) 330, 430
BmuI ACTGGG 2 cut(s) 330, 430
BpiI GAAGAC 1 cut(s) 377
BpmI CTGGAG 1 cut(s) 464
Bpu14I TTCGAA 1 cut(s) 761
BpuEI CTTGAG 1 cut(s) 942
BpuMI CCSGG 1 cut(s) 75
Bsa29I ATCGAT 1 cut(s) 138
BsaAI YACGTR 2 cut(s) 667, 801
BsaHI GRCGYC 1 cut(s) 620
BsaJI CCNNGG 3 cut(s) 74, 194, 354
Bsc4I CCNNNNNNNGG 3 cut(s) 629, 883, 1022
Bse1I ACTGG 6 cut(s) 277, 325, 425, 447, 553, 847
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 3 cut(s) 74, 194, 354
BseGI GGATG 2 cut(s) 88, 1065
BseLI CCNNNNNNNGG 3 cut(s) 629, 883, 1022
BseMII CTCAG 5 cut(s) 180, 269, 471, 872, 1008
BseNI ACTGG 6 cut(s) 277, 325, 425, 447, 553, 847
BseSI GKGCMC 1 cut(s) 672
BsgI GTGCAG 1 cut(s) 107
BshFI GGCC 2 cut(s) 887, 1033
BshNI GGYRCC 1 cut(s) 619
BshVI ATCGAT 1 cut(s) 138
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 953
BslI CCNNNNNNNGG 3 cut(s) 629, 883, 1022
BsmAI GTCTC 2 cut(s) 910, 960
BsmFI GGGAC 1 cut(s) 953
BsnI GGCC 2 cut(s) 887, 1033
Bsp119I TTCGAA 1 cut(s) 761
Bsp1286I GDGCHC 2 cut(s) 400, 672
Bsp143I GATC 3 cut(s) 55, 945, 1042
BspANI GGCC 2 cut(s) 887, 1033
BspCNI CTCAG 5 cut(s) 181, 270, 470, 873, 1009
BspDI ATCGAT 1 cut(s) 138
BspHI TCATGA 1 cut(s) 912
BspLI GGNNCC 3 cut(s) 567, 621, 634
BspMI ACCTGC 2 cut(s) 129, 655
BspQI GCTCTTC 1 cut(s) 96
BspT104I TTCGAA 1 cut(s) 761
BspT107I GGYRCC 1 cut(s) 619
BsrI ACTGG 6 cut(s) 277, 325, 425, 447, 553, 847
BssECI CCNNGG 3 cut(s) 74, 194, 354
BssMI GATC 3 cut(s) 55, 945, 1042
BssNI GRCGYC 1 cut(s) 620
BssSI CACGAG 1 cut(s) 792
BssT1I CCWWGG 2 cut(s) 194, 354
Bst2BI CACGAG 1 cut(s) 792
Bst6I CTCTTC 2 cut(s) 96, 1003
BstACI GRCGYC 1 cut(s) 620
BstBAI YACGTR 2 cut(s) 667, 801
BstBI TTCGAA 1 cut(s) 761
BstDEI CTNAG 5 cut(s) 189, 278, 457, 881, 1017
BstF5I GGATG 2 cut(s) 88, 1065
BstH2I RGCGCY 1 cut(s) 623
BstHHI GCGC 1 cut(s) 622
BstKTI GATC 3 cut(s) 58, 948, 1045
BstMAI GTCTC 2 cut(s) 910, 960
BstMBI GATC 3 cut(s) 55, 945, 1042
BstMWI GCNNNNNNNGC 2 cut(s) 32, 299
BstSCI CCNGG 1 cut(s) 73
BstSFI CTRYAG 1 cut(s) 385
BstSLI GKGCMC 1 cut(s) 672
BstV2I GAAGAC 1 cut(s) 377
BstXI CCANNNNNNTGG 1 cut(s) 548
Bsu15I ATCGAT 1 cut(s) 138
BsuI GTATCC 1 cut(s) 276
BsuRI GGCC 2 cut(s) 887, 1033
BsuTUI ATCGAT 1 cut(s) 138
BtsCI GGATG 2 cut(s) 88, 1065
BtsIMutI CAGTG 1 cut(s) 955
BveI ACCTGC 2 cut(s) 129, 655
CciI TCATGA 1 cut(s) 912
CfoI GCGC 1 cut(s) 622
Cfr13I GGNCC 2 cut(s) 566, 885
ClaI ATCGAT 1 cut(s) 138
Csp6I GTAC 1 cut(s) 238
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 8 cut(s) 364, 427, 431, 572, 686, 913, 943, 1006
CviJI RGCY 9 cut(s) 35, 106, 193, 302, 317, 398, 635, 887, 1033
CviKI_1 RGCY 9 cut(s) 35, 106, 193, 302, 317, 398, 635, 887, 1033
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 5 cut(s) 189, 278, 457, 881, 1017
DinI GGCGCC 1 cut(s) 621
DpnI GATC 3 cut(s) 57, 947, 1044
DpnII GATC 3 cut(s) 55, 945, 1042
Eam1104I CTCTTC 2 cut(s) 96, 1003
EarI CTCTTC 2 cut(s) 96, 1003
Eco130I CCWWGG 2 cut(s) 194, 354
Eco24I GRGCYC 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 566
Eco57I CTGAAG 2 cut(s) 318, 636
EcoO109I RGGNCCY 2 cut(s) 566, 885
EcoRI GAATTC 2 cut(s) 559, 808
EcoT14I CCWWGG 2 cut(s) 194, 354
EcoT22I ATGCAT 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 400
EgeI GGCGCC 1 cut(s) 621
EheI GGCGCC 1 cut(s) 621
ErhI CCWWGG 2 cut(s) 194, 354
FaeI CATG 8 cut(s) 367, 430, 434, 575, 689, 916, 946, 1009
FaqI GGGAC 1 cut(s) 953
FatI CATG 8 cut(s) 363, 426, 430, 571, 685, 912, 942, 1005
FbaI TGATCA 3 cut(s) 55, 945, 1042
FokI GGATG 1 cut(s) 75
FriOI GRGCYC 1 cut(s) 400
GlaI GCGC 1 cut(s) 621
GsuI CTGGAG 1 cut(s) 464
HaeII RGCGCY 1 cut(s) 623
HaeIII GGCC 2 cut(s) 887, 1033
HapII CCGG 1 cut(s) 74
HhaI GCGC 1 cut(s) 622
Hin1I GRCGYC 1 cut(s) 620
Hin1II CATG 8 cut(s) 367, 430, 434, 575, 689, 916, 946, 1009
Hin6I GCGC 1 cut(s) 620
HinP1I GCGC 1 cut(s) 620
HinfI GANTC 8 cut(s) 84, 140, 614, 656, 689, 698, 763, 795
HpaII CCGG 1 cut(s) 74
HphI GGTGA 3 cut(s) 179, 419, 508
Hpy166II GTNNAC 4 cut(s) 175, 463, 599, 985
Hpy188I TCNGA 8 cut(s) 60, 190, 270, 279, 655, 807, 855, 882
Hpy188III TCNNGA 4 cut(s) 913, 959, 1016, 1046
Hpy8I GTNNAC 4 cut(s) 175, 463, 599, 985
HpyAV CCTTC 3 cut(s) 124, 223, 777
HpyCH4IV ACGT 2 cut(s) 666, 800
HpyCH4V TGCA 6 cut(s) 7, 124, 156, 430, 482, 871
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 299
HpyF3I CTNAG 5 cut(s) 189, 278, 457, 881, 1017
HpySE526I ACGT 2 cut(s) 666, 800
Hsp92I GRCGYC 1 cut(s) 620
Hsp92II CATG 8 cut(s) 367, 430, 434, 575, 689, 916, 946, 1009
HspAI GCGC 1 cut(s) 620
KasI GGCGCC 1 cut(s) 619
Ksp22I TGATCA 3 cut(s) 55, 945, 1042
Kzo9I GATC 3 cut(s) 55, 945, 1042
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 2 cut(s) 632, 829
MaeII ACGT 2 cut(s) 666, 800
MaeIII GTNAC 1 cut(s) 796
MalI GATC 3 cut(s) 57, 947, 1044
MboI GATC 3 cut(s) 55, 945, 1042
MboII GAAGA 7 cut(s) 62, 113, 379, 382, 582, 750, 990
MfeI CAATTG 1 cut(s) 162
MhlI GDGCHC 2 cut(s) 400, 672
MluCI AATT 6 cut(s) 162, 452, 559, 661, 808, 841
Mly113I GGCGCC 1 cut(s) 620
MlyI GAGTC 4 cut(s) 608, 665, 698, 804
MmeI TCCRAC 5 cut(s) 116, 168, 293, 591, 705
Mph1103I ATGCAT 1 cut(s) 432
MseI TTAA 1 cut(s) 866
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 1 cut(s) 75
MunI CAATTG 1 cut(s) 162
MwoI GCNNNNNNNGC 2 cut(s) 32, 299
NarI GGCGCC 1 cut(s) 620
NciI CCSGG 1 cut(s) 75
NdeII GATC 3 cut(s) 55, 945, 1042
NlaIII CATG 8 cut(s) 367, 430, 434, 575, 689, 916, 946, 1009
NlaIV GGNNCC 3 cut(s) 567, 621, 634
NmeAIII GCCGAG 1 cut(s) 596
NmuCI GTSAC 1 cut(s) 796
NsiI ATGCAT 1 cut(s) 432
NspV TTCGAA 1 cut(s) 761
PagI TCATGA 1 cut(s) 912
PciSI GCTCTTC 1 cut(s) 96
PfeI GAWTC 4 cut(s) 84, 140, 698, 763
PleI GAGTC 4 cut(s) 608, 664, 697, 803
PluTI GGCGCC 1 cut(s) 623
PpsI GAGTC 4 cut(s) 608, 664, 697, 803
Ppu21I YACGTR 2 cut(s) 667, 801
PpuMI RGGWCCY 1 cut(s) 566
Psp5II RGGWCCY 1 cut(s) 566
PspN4I GGNNCC 3 cut(s) 567, 621, 634
PspPI GGNCC 2 cut(s) 566, 885
PspPPI RGGWCCY 1 cut(s) 566
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SapI GCTCTTC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 866
Sau3AI GATC 3 cut(s) 55, 945, 1042
Sau96I GGNCC 2 cut(s) 566, 885
SchI GAGTC 4 cut(s) 608, 665, 698, 804
ScrFI CCNGG 1 cut(s) 75
SduI GDGCHC 2 cut(s) 400, 672
SfcI CTRYAG 1 cut(s) 385
SfoI GGCGCC 1 cut(s) 621
SfuI TTCGAA 1 cut(s) 761
SinI GGWCC 1 cut(s) 566
SmlI CTYRAG 1 cut(s) 957
SmoI CTYRAG 1 cut(s) 957
Sse9I AATT 6 cut(s) 162, 452, 559, 661, 808, 841
SspDI GGCGCC 1 cut(s) 619
StyD4I CCNGG 1 cut(s) 73
StyI CCWWGG 2 cut(s) 194, 354
TaiI ACGT 2 cut(s) 669, 803
TaqI TCGA 4 cut(s) 82, 138, 659, 761
TasI AATT 6 cut(s) 162, 452, 559, 661, 808, 841
TfiI GAWTC 4 cut(s) 84, 140, 698, 763
Tru1I TTAA 1 cut(s) 866
Tru9I TTAA 1 cut(s) 866
TscAI CASTG 1 cut(s) 955
TseFI GTSAC 1 cut(s) 796
Tsp45I GTSAC 1 cut(s) 796
TspDTI ATGAA 5 cut(s) 114, 380, 447, 822, 901
TspRI CASTG 1 cut(s) 955
VpaK11BI GGWCC 1 cut(s) 566
XapI RAATTY 4 cut(s) 452, 559, 808, 841
Zsp2I ATGCAT 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.