AT4G13160

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008250
Physical Location & Seq
Forward (+)
7653594 .. 7654969
1376 bp
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UTR
Exon/CDS
Intron
AT4G13160.1

Sequence Viewer

Length: 849 bp
ATGGACTACCAAGAAAGTTATAGATTGACATTTTATGGAATTTTGGTTGCTTTTATGGAATTAGCATTTGCTTATTGTCTTCTATGCGTATCAGCTTTCGTCTTTATTACATCGAAGTTGCTACTGTTTATGCCATGTATCGGGTACCAAAACAGTGATCTCTGCATCCAAAAACTTCTCTTTGATTGGCCATTCAGAATCATACTCAGAGTTCAGAAGCTAGCTACTACTAATAGGCCAAGCGTTTTACATCATCAAGAACAAGAACAAGAACAAGAACAAGAACAAGAAGAGAAGACTATGGTTGATAAAGATAAGAACTCGGAATTGATGGATAGAGTTAGGTTGTTGGAAGTAGCTGTTGAGCAAGAGAAAGTAGCTAAAGCTGCTTTAATGGTTGAATTGGAACAAGAAAGAGCAGCTTCAGCATCAGCAGCTGATGAAGCTATGGCTATGATTTTAAGGCTACAAGCGGATAAAGCTTCGCTTGAGATGGAAGGGAAGCAATATGAGAGAATGATTGATGAGAAATTTGCTTATGATGAGGAAGAGATGAATATTCTCAAGGAGATTCTTTTTAAAAGGGAGAGAGAAAAGCATTTTCTTGAGAAAGAACTTGAGACTTACAAGCACATTGATGATGATCAAGAGACGGAAGACAACGAGAAGAGAGAATGCGATGAGGATGGAGAACCGATTGTGTATGATGTTCATGTCATTGAGGATAATAGTCAGACTAAAGTGAGGGATGATGATGTAGCAGAACACAAAGAGATGAAGGAAGAAGAGATAGTCAAGGATCATGATTCAGTATCATCACCTACTTCATCTTCAACACATCTCCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

282

Amino Acids

33.07

Weight (kDa)

4.58

Isoelectric Point (pI)

54.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 117 - 206 2.7e-30 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 144
AccB1I GGYRCC 1 cut(s) 144
AciI CCGC 1 cut(s) 473
AclWI GGATC 1 cut(s) 807
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 2 cut(s) 39, 530
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 1 cut(s) 140
AgsI TTSAA 2 cut(s) 401, 834
Alw26I GTCTC 2 cut(s) 614, 644
AlwI GGATC 1 cut(s) 807
AlwNI CAGNNNCTG 1 cut(s) 437
AoxI GGCC 2 cut(s) 188, 236
ApeKI GCWGC 3 cut(s) 386, 419, 434
ApoI RAATTY 2 cut(s) 39, 530
Asp718I GGTACC 1 cut(s) 144
AsuHPI GGTGA 1 cut(s) 810
AsuNHI GCTAGC 1 cut(s) 220
BalI TGGCCA 1 cut(s) 190
BanI GGYRCC 1 cut(s) 144
BbsI GAAGAC 3 cut(s) 71, 302, 663
BbvI GCAGC 3 cut(s) 373, 431, 446
BccI CCATC 3 cut(s) 325, 487, 680
BclI TGATCA 1 cut(s) 643
BcoDI GTCTC 2 cut(s) 614, 644
BfaI CTAG 1 cut(s) 221
BisI GCNGC 3 cut(s) 387, 420, 435
BlsI GCNGC 3 cut(s) 388, 421, 436
BmiI GGNNCC 1 cut(s) 146
BmsI GCATC 2 cut(s) 174, 437
BmtI GCTAGC 1 cut(s) 224
BpiI GAAGAC 3 cut(s) 71, 302, 663
BpuEI CTTGAG 4 cut(s) 509, 548, 626, 638
BsaBI GATNNNNATC 1 cut(s) 642
Bsc4I CCNNNNNNNGG 1 cut(s) 140
Bse8I GATNNNNATC 1 cut(s) 642
BseGI GGATG 3 cut(s) 165, 691, 754
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 1 cut(s) 140
BseMII CTCAG 1 cut(s) 220
BseXI GCAGC 3 cut(s) 373, 431, 446
BshFI GGCC 2 cut(s) 190, 238
BshNI GGYRCC 1 cut(s) 144
BslI CCNNNNNNNGG 1 cut(s) 140
BsmAI GTCTC 2 cut(s) 614, 644
BsmBI CGTCTC 1 cut(s) 644
BsmI GAATGC 1 cut(s) 680
BsnI GGCC 2 cut(s) 190, 238
Bsp143I GATC 3 cut(s) 157, 643, 799
BspACI CCGC 1 cut(s) 473
BspANI GGCC 2 cut(s) 190, 238
BspCNI CTCAG 1 cut(s) 219
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 1 cut(s) 146
BspOI GCTAGC 1 cut(s) 224
BspPI GGATC 1 cut(s) 807
BspT107I GGYRCC 1 cut(s) 144
BssMI GATC 3 cut(s) 157, 643, 799
Bst4CI ACNGT 2 cut(s) 126, 155
Bst6I CTCTTC 4 cut(s) 285, 543, 662, 780
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 1 cut(s) 206
BstF5I GGATG 3 cut(s) 165, 691, 754
BstKTI GATC 3 cut(s) 160, 646, 802
BstMAI GTCTC 2 cut(s) 614, 644
BstMBI GATC 3 cut(s) 157, 643, 799
BstMWI GCNNNNNNNGC 5 cut(s) 386, 425, 434, 443, 479
BstV1I GCAGC 3 cut(s) 373, 431, 446
BstV2I GAAGAC 3 cut(s) 71, 302, 663
BsuRI GGCC 2 cut(s) 190, 238
BtgZI GCGATG 1 cut(s) 693
BtsCI GGATG 3 cut(s) 165, 691, 754
BtsIMutI CAGTG 1 cut(s) 160
Cac8I GCNNGC 1 cut(s) 222
CaiI CAGNNNCTG 1 cut(s) 437
CciI TCATGA 1 cut(s) 802
Csp6I GTAC 1 cut(s) 145
CviAII CATG 3 cut(s) 135, 713, 803
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 1 cut(s) 206
DpnI GATC 3 cut(s) 159, 645, 801
DpnII GATC 3 cut(s) 157, 643, 799
DraI TTTAAA 1 cut(s) 580
EaeI YGGCCR 1 cut(s) 188
Eam1104I CTCTTC 4 cut(s) 285, 543, 662, 780
EarI CTCTTC 4 cut(s) 285, 543, 662, 780
Eco57I CTGAAG 1 cut(s) 408
Esp3I CGTCTC 1 cut(s) 644
FaeI CATG 3 cut(s) 138, 716, 806
FalI AAGNNNNNCTT 4 cut(s) 406, 438, 471, 503
FatI CATG 3 cut(s) 134, 712, 802
FbaI TGATCA 1 cut(s) 643
Fnu4HI GCNGC 3 cut(s) 387, 420, 435
FokI GGATG 3 cut(s) 152, 698, 761
Fsp4HI GCNGC 3 cut(s) 387, 420, 435
FspBI CTAG 1 cut(s) 221
GluI GCNGC 3 cut(s) 387, 420, 435
HaeIII GGCC 2 cut(s) 190, 238
Hin1II CATG 3 cut(s) 138, 716, 806
HindIII AAGCTT 1 cut(s) 480
HinfI GANTC 3 cut(s) 198, 571, 806
HphI GGTGA 1 cut(s) 810
Hpy188I TCNGA 5 cut(s) 197, 209, 216, 325, 735
Hpy188III TCNNGA 4 cut(s) 257, 605, 647, 803
HpyAV CCTTC 2 cut(s) 491, 772
HpyCH4III ACNGT 2 cut(s) 126, 155
HpyCH4V TGCA 1 cut(s) 165
HpyF10VI GCNNNNNNNGC 5 cut(s) 386, 425, 434, 443, 479
HpyF3I CTNAG 1 cut(s) 206
Hsp92II CATG 3 cut(s) 138, 716, 806
KpnI GGTACC 1 cut(s) 148
Ksp22I TGATCA 1 cut(s) 643
Kzo9I GATC 3 cut(s) 157, 643, 799
Lsp1109I GCAGC 3 cut(s) 373, 431, 446
LweI GCATC 2 cut(s) 174, 437
MaeI CTAG 1 cut(s) 221
MalI GATC 3 cut(s) 159, 645, 801
MboI GATC 3 cut(s) 157, 643, 799
MboII GAAGA 9 cut(s) 71, 302, 307, 560, 668, 679, 794, 797, 822
MlsI TGGCCA 1 cut(s) 190
MluCI AATT 5 cut(s) 39, 59, 326, 401, 530
MluNI TGGCCA 1 cut(s) 190
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 4 cut(s) 538, 676, 715, 738
Mox20I TGGCCA 1 cut(s) 190
MscI TGGCCA 1 cut(s) 190
MseI TTAA 3 cut(s) 392, 461, 579
MslI CAYNNNNRTG 1 cut(s) 636
Msp20I TGGCCA 1 cut(s) 190
MspA1I CMGCKG 1 cut(s) 437
Mva1269I GAATGC 1 cut(s) 680
MwoI GCNNNNNNNGC 5 cut(s) 386, 425, 434, 443, 479
NdeII GATC 3 cut(s) 157, 643, 799
NheI GCTAGC 1 cut(s) 220
NlaIII CATG 3 cut(s) 138, 716, 806
NlaIV GGNNCC 1 cut(s) 146
PagI TCATGA 1 cut(s) 802
PctI GAATGC 1 cut(s) 680
PfeI GAWTC 3 cut(s) 198, 571, 806
PkrI GCNGC 3 cut(s) 388, 421, 436
PspN4I GGNNCC 1 cut(s) 146
PstNI CAGNNNCTG 1 cut(s) 437
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 636
SaqAI TTAA 3 cut(s) 392, 461, 579
SatI GCNGC 3 cut(s) 387, 420, 435
Sau3AI GATC 3 cut(s) 157, 643, 799
SfaNI GCATC 2 cut(s) 174, 437
SmiMI CAYNNNNRTG 1 cut(s) 636
SmlI CTYRAG 4 cut(s) 488, 563, 605, 617
SmoI CTYRAG 4 cut(s) 488, 563, 605, 617
Sse9I AATT 5 cut(s) 39, 59, 326, 401, 530
SsiI CCGC 1 cut(s) 473
SspI AATATT 1 cut(s) 559
SspMI CTAG 1 cut(s) 221
TaaI ACNGT 2 cut(s) 126, 155
TaqI TCGA 1 cut(s) 113
TasI AATT 5 cut(s) 39, 59, 326, 401, 530
TfiI GAWTC 3 cut(s) 198, 571, 806
Tru1I TTAA 3 cut(s) 392, 461, 579
Tru9I TTAA 3 cut(s) 392, 461, 579
TscAI CASTG 1 cut(s) 160
TseI GCWGC 3 cut(s) 386, 419, 434
TspDTI ATGAA 5 cut(s) 456, 569, 701, 791, 816
TspGWI ACGGA 1 cut(s) 668
TspRI CASTG 1 cut(s) 160
XapI RAATTY 2 cut(s) 39, 530
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.