Rh6CG281900

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0008250
Physical Location & Seq
Reverse (-)
45938907 .. 45943069
4163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG281900.1

Sequence Viewer

Length: 1614 bp
ATGGCTTGTCAGGAAGTTCAATCATGGAGCTTTGGTGGTCTTACAGTAGCATTTCTTGACCTTTTACTTGCTTATTTCCTGCTATGCATATCAGCTTTTGTGTTTTTCGTATCGAAGTTGTTGAAGGTTTTCTGGGTGTATTTGCCTTGTCCTTGTAATGGGGTTTTCGGGTATAGAAATCGTGATCTTTGCTTGCATAAGTTGCTTAATGAGTGGCCTGTTGCCAAGATTTATGCTGTTCAGAAGTTGGTGAAGAGTAGGTTCCCTTTTGATATTCGGTTTAAGGATCAAACATGCAACTTGAGTCAGAAGTTGATTAGGGATGTGAATGGAGGGAATGGTGTTCTTGAATTGGAAGGTGAGTCATGTTGTAGTCCGTTTTCGAGCCCGAGATTGCAAAGTCTGGTTGATAAGGAAAGTGGGTATGATGCTAAGGGGAAGAGAATTGTGATTCTGAAGAAAAGGACTGGAATTCGGCGTTCTAGGAGGGATAGTTCAAAATCTCCTCGTATATTCCCCTTTCCTCTTGATGAAAGAGGTACAGGAGAGATATCTGGTGAAAGCTCAATTGCTGTAGCTGCAAGACACGATGAGCCTCAAGCATCCGGAGATGATAGAGGGGAAGGAATATGTGGACTTTCTAAGTTGAGTGAATCAGCTGCTGAGAGTAAAGGTCTGGATGCAAGTTACGGTGAAGAAAAGTTTCCCATTTTTGAAAATGAAAGTGATACAATCAGGATGTTGGAAGGAGCACTTGAAAAAGAGAAAGCTGCTTGTGCTGCTCTCTATTTAGAGCTGGAGAAAGAGCGATCTGCTGCTGCAACTGCTGCTGATGAAGCAATGGCCATGATAAGTCGTCTGCAGAAGGATAAAGCATCGACAGAAATGGAAGTGAGACAGTACCAAAGGATGATTGAAGAGAAATTTGTTTATGATGAAGAAGAGATGGATGTTCTGAAAGAGATACTTCTTAGGAGGGAGAAGGAGAATCATTATTTGGAGAAGGAAGTTGAAGCATATAGACAGATGAATTCTTTAGGAAATGAACAGTGTAATGGTGATTCAAATGATAAGCTGCGTGAATGGGGACAAACTGCACTTTCTTCGAATATAGATTCACAACTGATGATGCCGTGGACTAATGAAACTAAATCTAATCATATGGAACTGGGATATATTGCAGACAGTACCTCTCAGTTTGACGCTTCATTTGTCAAAAAACAAATCCATTCCAATGGACATGATGTTGTTGAGAAAAGTGTCCTTTCAGGATGGGAAGGAAAACAATACGCAGATAATGCAATGTGCCCAGGAATGACTACTGAAGCAGTCCAAGCTAAGATAGGGATTGAGAAAAGTTTCTATAGTGATGGAGGAGAGCTGCAGCAAGGTGGGGATCTTGAAAGCCATTATCATAGTCCATTGCTTGATACAGAGCCAGCTGTTCATGACATTCATGTCATAGATGGTAAAACTGAAATAGTAAGAGGAGAAAGTAGGCCATCAATTTATGCTGCTTTGGATGGATTCCAAGATCCGAGATGTGGGGCTTCTGGAGTCTGTAGCAGCTTGGAACCTCTGCTGAGCATGGGGAAAGGCAAAACGCCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

537

Amino Acids

59.88

Weight (kDa)

5.22

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 248 - 337 2.6e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1457
AccIII TCCGGA 1 cut(s) 605
AclWI GGATC 3 cut(s) 294, 1404, 1529
AcoI YGGCCR 1 cut(s) 843
AcsI RAATTY 3 cut(s) 471, 923, 1030
AcuI CTGAAG 2 cut(s) 476, 1344
AfaI GTAC 3 cut(s) 541, 902, 1189
AfiI CCNNNNNNNGG 2 cut(s) 158, 1544
AjnI CCWGG 1 cut(s) 1309
AjuI GAANNNNNNNTTGG 2 cut(s) 980, 1012
AloI GAACNNNNNNTCC 2 cut(s) 478, 510
Alw21I GWGCWC 1 cut(s) 754
Alw26I GTCTC 1 cut(s) 889
AlwI GGATC 3 cut(s) 294, 1404, 1529
AlwNI CAGNNNCTG 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 388
Aor13HI TCCGGA 1 cut(s) 605
AoxI GGCC 3 cut(s) 215, 843, 1499
ApoI RAATTY 3 cut(s) 471, 923, 1030
ArsI GACNNNNNNTTYG 2 cut(s) 1193, 1225
Asp700I GAANNNNTTC 3 cut(s) 128, 702, 1358
AsuHPI GGTGA 5 cut(s) 262, 371, 569, 704, 1070
AsuII TTCGAA 1 cut(s) 1106
AvaI CYCGRG 1 cut(s) 388
BaeGI GKGCMC 1 cut(s) 1310
BalI TGGCCA 1 cut(s) 845
BanII GRGCYC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 754
BccI CCATC 6 cut(s) 940, 1266, 1364, 1460, 1510, 1517
BceAI ACGGC 1 cut(s) 1117
BcgI CGANNNNNNTGC 2 cut(s) 1086, 1120
BciT130I CCWGG 1 cut(s) 1311
BcoDI GTCTC 1 cut(s) 889
BfaI CTAG 1 cut(s) 483
BfmI CTRYAG 5 cut(s) 573, 860, 1363, 1382, 1561
BlpI GCTNAGC 1 cut(s) 1583
Bme1390I CCNGG 1 cut(s) 1311
BmeT110I CYCGRG 1 cut(s) 388
BmiI GGNNCC 2 cut(s) 263, 1575
BmrFI CCNGG 1 cut(s) 1311
BmrI ACTGGG 1 cut(s) 1178
BmsI GCATC 5 cut(s) 418, 611, 670, 884, 1119
BmuI ACTGGG 1 cut(s) 1178
BpmI CTGGAG 2 cut(s) 818, 1575
Bpu10I CCTNAGC 1 cut(s) 432
Bpu1102I GCTNAGC 1 cut(s) 1583
Bpu14I TTCGAA 1 cut(s) 1106
BpuEI CTTGAG 2 cut(s) 322, 582
BsaJI CCNNGG 2 cut(s) 1133, 1309
BsaWI WCCGGW 1 cut(s) 605
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 478, 508
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 1544
Bse1I ACTGG 2 cut(s) 472, 1173
Bse3DI GCAATG 3 cut(s) 846, 1308, 1421
BseAI TCCGGA 1 cut(s) 605
BseBI CCWGG 1 cut(s) 1311
BseDI CCNNGG 2 cut(s) 1133, 1309
BseGI GGATG 8 cut(s) 328, 602, 685, 744, 915, 955, 1277, 1528
BseLI CCNNNNNNNGG 2 cut(s) 158, 1544
BseMI GCAATG 3 cut(s) 846, 1308, 1421
BseMII CTCAG 3 cut(s) 654, 1208, 1574
BseNI ACTGG 2 cut(s) 472, 1173
BseRI GAGGAG 3 cut(s) 495, 1389, 1503
BseSI GKGCMC 1 cut(s) 1310
BsgI GTGCAG 1 cut(s) 1080
BshFI GGCC 3 cut(s) 217, 845, 1501
BsiHKAI GWGCWC 1 cut(s) 754
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 1 cut(s) 606
BslFI GGGAC 1 cut(s) 1101
BslI CCNNNNNNNGG 2 cut(s) 158, 1544
BsmAI GTCTC 1 cut(s) 889
BsmFI GGGAC 1 cut(s) 1101
BsnI GGCC 3 cut(s) 217, 845, 1501
BsoBI CYCGRG 1 cut(s) 388
Bsp119I TTCGAA 1 cut(s) 1106
Bsp1286I GDGCHC 3 cut(s) 389, 754, 1310
Bsp13I TCCGGA 1 cut(s) 605
Bsp143I GATC 5 cut(s) 184, 286, 809, 1396, 1534
Bsp1720I GCTNAGC 1 cut(s) 1583
BspANI GGCC 3 cut(s) 217, 845, 1501
BspCNI CTCAG 3 cut(s) 655, 1207, 1575
BspEI TCCGGA 1 cut(s) 605
BspHI TCATGA 1 cut(s) 1447
BspLI GGNNCC 2 cut(s) 263, 1575
BspMAI CTGCAG 2 cut(s) 864, 1386
BspPI GGATC 3 cut(s) 294, 1404, 1529
BspT104I TTCGAA 1 cut(s) 1106
BsrDI GCAATG 3 cut(s) 846, 1308, 1421
BsrI ACTGG 2 cut(s) 472, 1173
BssECI CCNNGG 2 cut(s) 1133, 1309
BssMI GATC 5 cut(s) 184, 286, 809, 1396, 1534
Bst2UI CCWGG 1 cut(s) 1311
Bst4CI ACNGT 5 cut(s) 46, 692, 900, 1050, 1187
Bst6I CTCTTC 4 cut(s) 248, 434, 912, 936
BstAPI GCANNNNNTGC 3 cut(s) 202, 827, 1298
BstBI TTCGAA 1 cut(s) 1106
BstC8I GCNNGC 2 cut(s) 194, 1440
BstDEI CTNAG 7 cut(s) 432, 642, 663, 971, 1194, 1338, 1583
BstDSI CCRYGG 1 cut(s) 1133
BstF5I GGATG 8 cut(s) 328, 602, 685, 744, 915, 955, 1277, 1528
BstKTI GATC 5 cut(s) 187, 289, 812, 1399, 1537
BstMAI GTCTC 1 cut(s) 889
BstMBI GATC 5 cut(s) 184, 286, 809, 1396, 1534
BstMWI GCNNNNNNNGC 9 cut(s) 202, 578, 776, 779, 824, 827, 836, 1298, 1334
BstNI CCWGG 1 cut(s) 1311
BstNSI RCATGY 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 1309
BstSFI CTRYAG 5 cut(s) 573, 860, 1363, 1382, 1561
BstSLI GKGCMC 1 cut(s) 1310
BstX2I RGATCY 2 cut(s) 1396, 1534
BstXI CCANNNNNNTGG 1 cut(s) 1235
BstYI RGATCY 2 cut(s) 1396, 1534
BsuRI GGCC 3 cut(s) 217, 845, 1501
BtgI CCRYGG 1 cut(s) 1133
BtsCI GGATG 8 cut(s) 328, 602, 685, 744, 915, 955, 1277, 1528
BtsIMutI CAGTG 1 cut(s) 1055
Cac8I GCNNGC 2 cut(s) 194, 1440
CaiI CAGNNNCTG 1 cut(s) 662
CciI TCATGA 1 cut(s) 1447
CseI GACGC 1 cut(s) 1211
Csp6I GTAC 3 cut(s) 540, 901, 1188
CviAII CATG 8 cut(s) 24, 294, 366, 847, 1241, 1448, 1457, 1588
CviQI GTAC 3 cut(s) 540, 901, 1188
DdeI CTNAG 7 cut(s) 432, 642, 663, 971, 1194, 1338, 1583
DpnI GATC 5 cut(s) 186, 288, 811, 1398, 1536
DpnII GATC 5 cut(s) 184, 286, 809, 1396, 1534
DrdI GACNNNNNNGTC 1 cut(s) 1457
DseDI GACNNNNNNGTC 1 cut(s) 1457
EaeI YGGCCR 1 cut(s) 843
Eam1104I CTCTTC 4 cut(s) 248, 434, 912, 936
EarI CTCTTC 4 cut(s) 248, 434, 912, 936
Eco24I GRGCYC 1 cut(s) 389
Eco32I GATATC 1 cut(s) 552
Eco57I CTGAAG 2 cut(s) 476, 1344
Eco88I CYCGRG 1 cut(s) 388
EcoRI GAATTC 2 cut(s) 471, 1030
EcoRII CCWGG 1 cut(s) 1309
EcoRV GATATC 1 cut(s) 552
EcoT22I ATGCAT 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 389
FaeI CATG 8 cut(s) 27, 297, 369, 850, 1244, 1451, 1460, 1591
FalI AAGNNNNNCTT 4 cut(s) 738, 770, 951, 983
FaqI GGGAC 1 cut(s) 1101
FatI CATG 8 cut(s) 23, 293, 365, 846, 1240, 1447, 1456, 1587
FauNDI CATATG 1 cut(s) 1161
FokI GGATG 8 cut(s) 335, 589, 692, 751, 922, 962, 1284, 1535
FriOI GRGCYC 1 cut(s) 389
FspBI CTAG 1 cut(s) 483
GsuI CTGGAG 2 cut(s) 818, 1575
HaeIII GGCC 3 cut(s) 217, 845, 1501
HapII CCGG 1 cut(s) 606
HgaI GACGC 1 cut(s) 1211
Hin1II CATG 8 cut(s) 27, 297, 369, 850, 1244, 1451, 1460, 1591
HinfI GANTC 9 cut(s) 304, 362, 451, 653, 988, 1061, 1115, 1527, 1557
HpaII CCGG 1 cut(s) 606
HphI GGTGA 5 cut(s) 262, 371, 569, 704, 1070
Hpy166II GTNNAC 2 cut(s) 635, 1137
Hpy188I TCNGA 5 cut(s) 243, 309, 456, 957, 1539
Hpy8I GTNNAC 2 cut(s) 635, 1137
HpyAV CCTTC 8 cut(s) 118, 350, 617, 740, 859, 976, 997, 1271
HpyCH4III ACNGT 5 cut(s) 46, 692, 900, 1050, 1187
HpyF10VI GCNNNNNNNGC 9 cut(s) 202, 578, 776, 779, 824, 827, 836, 1298, 1334
HpyF3I CTNAG 7 cut(s) 432, 642, 663, 971, 1194, 1338, 1583
Hsp92II CATG 8 cut(s) 27, 297, 369, 850, 1244, 1451, 1460, 1591
Kpn2I TCCGGA 1 cut(s) 605
Kzo9I GATC 5 cut(s) 184, 286, 809, 1396, 1534
LmnI GCTCC 2 cut(s) 27, 749
LweI GCATC 5 cut(s) 418, 611, 670, 884, 1119
MaeI CTAG 1 cut(s) 483
MaeIII GTNAC 1 cut(s) 686
MalI GATC 5 cut(s) 186, 288, 811, 1398, 1536
MboI GATC 5 cut(s) 184, 286, 809, 1396, 1534
MboII GAAGA 8 cut(s) 265, 451, 469, 707, 929, 950, 953, 1095
MfeI CAATTG 1 cut(s) 567
MflI RGATCY 2 cut(s) 1396, 1534
MhlI GDGCHC 3 cut(s) 389, 754, 1310
MlsI TGGCCA 1 cut(s) 845
MluCI AATT 7 cut(s) 350, 444, 471, 567, 923, 1030, 1506
MluNI TGGCCA 1 cut(s) 845
MlyI GAGTC 3 cut(s) 313, 371, 1566
MmeI TCCRAC 1 cut(s) 723
Mox20I TGGCCA 1 cut(s) 845
Mph1103I ATGCAT 1 cut(s) 89
MroI TCCGGA 1 cut(s) 605
MroXI GAANNNNTTC 3 cut(s) 128, 702, 1358
MscI TGGCCA 1 cut(s) 845
MseI TTAA 3 cut(s) 207, 282, 1612
MslI CAYNNNNRTG 1 cut(s) 1233
Msp20I TGGCCA 1 cut(s) 845
MspA1I CMGCKG 2 cut(s) 659, 1442
MspI CCGG 1 cut(s) 606
MspR9I CCNGG 1 cut(s) 1311
MunI CAATTG 1 cut(s) 567
MvaI CCWGG 1 cut(s) 1311
MwoI GCNNNNNNNGC 9 cut(s) 202, 578, 776, 779, 824, 827, 836, 1298, 1334
NdeI CATATG 1 cut(s) 1161
NdeII GATC 5 cut(s) 184, 286, 809, 1396, 1534
NlaIII CATG 8 cut(s) 27, 297, 369, 850, 1244, 1451, 1460, 1591
NlaIV GGNNCC 2 cut(s) 263, 1575
NsiI ATGCAT 1 cut(s) 89
NspI RCATGY 1 cut(s) 297
NspV TTCGAA 1 cut(s) 1106
PagI TCATGA 1 cut(s) 1447
PdmI GAANNNNTTC 3 cut(s) 128, 702, 1358
PfeI GAWTC 6 cut(s) 451, 653, 988, 1061, 1115, 1527
PleI GAGTC 3 cut(s) 312, 370, 1565
PpsI GAGTC 3 cut(s) 312, 370, 1565
Psp6I CCWGG 1 cut(s) 1309
PspGI CCWGG 1 cut(s) 1309
PspN4I GGNNCC 2 cut(s) 263, 1575
PstI CTGCAG 2 cut(s) 864, 1386
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 2 cut(s) 1396, 1534
PvuII CAGCTG 2 cut(s) 659, 1442
RsaI GTAC 3 cut(s) 541, 902, 1189
RsaNI GTAC 3 cut(s) 540, 901, 1188
RseI CAYNNNNRTG 1 cut(s) 1233
SaqAI TTAA 3 cut(s) 207, 282, 1612
Sau3AI GATC 5 cut(s) 184, 286, 809, 1396, 1534
SchI GAGTC 3 cut(s) 313, 371, 1566
ScrFI CCNGG 1 cut(s) 1311
SduI GDGCHC 3 cut(s) 389, 754, 1310
SfaNI GCATC 5 cut(s) 418, 611, 670, 884, 1119
SfcI CTRYAG 5 cut(s) 573, 860, 1363, 1382, 1561
SfuI TTCGAA 1 cut(s) 1106
SmiMI CAYNNNNRTG 1 cut(s) 1233
SmlI CTYRAG 2 cut(s) 301, 597
SmoI CTYRAG 2 cut(s) 301, 597
Sse9I AATT 7 cut(s) 350, 444, 471, 567, 923, 1030, 1506
SspMI CTAG 1 cut(s) 483
StyD4I CCNGG 1 cut(s) 1309
TaaI ACNGT 5 cut(s) 46, 692, 900, 1050, 1187
TaqI TCGA 4 cut(s) 113, 383, 878, 1106
TasI AATT 7 cut(s) 350, 444, 471, 567, 923, 1030, 1506
TfiI GAWTC 6 cut(s) 451, 653, 988, 1061, 1115, 1527
Tru1I TTAA 3 cut(s) 207, 282, 1612
Tru9I TTAA 3 cut(s) 207, 282, 1612
TscAI CASTG 1 cut(s) 1055
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 1 cut(s) 1055
XapI RAATTY 3 cut(s) 471, 923, 1030
XceI RCATGY 1 cut(s) 297
XmnI GAANNNNTTC 3 cut(s) 128, 702, 1358
XspI CTAG 1 cut(s) 483
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.