Rroxscaffold_7G00184360

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008250
Physical Location & Seq
Forward (+)
23576180 .. 23578677
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00184360.1

Sequence Viewer

Length: 1530 bp
ATGGCTTGTCAGGAAGTTCAATCATGGAGCTTTGGTGGTCTTACAGTAGCATTTCTTGACCTTTTACTTGCTTATTTCCTGCTATGCATATCAGCTTTTGTGTTTTTCGTATCGAAGTTGTTGAAGGTTTTCTGGGTGTATTTGCCTTGTCCTTGTAATGGGGTTTTCGGGTATAGAAATCCTGATCTTTGCTTGCATAAGTTGCTTAATGAGTGGCCTGTTGCCAAGATTTATGCTGTTCAGAAGTTGGTGAAGAGTAGGTTCCCTTTTGATATTCGGTTTAAGGATCAAACATGCAACTTGAGTCAGAAGTTGATTAGGGATGTAAATGGAGGGAATGGTGTTCTTGAATTGGAAGGTGAGTCATGTTGTAGTCCGTTTTCGAGTCCAAGATTGCAAAGTCTGGTTGATAAGGAAAGTGGGTATGATGCTAAGGGGAAGAGAATTGTTATTCTGAAGAAAAGGACTGGAATTCGGCGTTCTAGGAGGGATAGTTCAAAATCTCCTCGTGTATTCCCCTTTCCTCTGGATGAAAGAGGTACAAGAGAGATATTTGGTGAAAGCTCAATTGCTGTAGCTGCAAGACACGATGAGCCTCAAGCATCCGGAGATGATAGAGGGGAAGGAATATGTGGACTTTCTAAGTTGAGTGAATCAGCTGCTGAGAGTAAAGGTCTGGATGCAAGTTACGGTGAAGAAAAGTATCCCATTTTTGAAAATGAAAGTGATACAATCAGGATGTTGGAAGGAGCACTCGAAAAAGAGAGGTCTGCTAGTGCTGCTCTCTATTTAGAGCTGGAGAAAGAGCGAGCTGCTGCTGCAACTGCTGCTGATGAAGCAATGGCCATGATAAGTCGTCTGCAGAAGGATAAAGCATCGACAGAAATGGAAGAGGGAGAAGGAGAATCATTTTTGGAGAAGGAAGTTGAAGCATATAGACAGATGAATTCTTTAGGAAATGAACACTGTAATGGTGATTCAAATGATAAGCTGCATGAATGGGGACAAACTGCACTTTCTTCGAATATAGATTCACAACTGATGATGCTGTGGACTAATGAAACTAAATCTAATCATATGGAAGTGGGAAATATTGCAGACCGTACCTCTCAGTTTGACGCTTCATTTGTCAAAAAACAAATCCATTCCAATGGACATGATGTTGTTGAGAAAAGTGTCCTTTCAGGATGGGAAGGAAAACTACACGCAGATAATGCAATGTGCCCAGGAATGACTACTGAAGCAGTCCAAGCTAAGATAGGGATTGAGAAAAGTTTCTATAGTGATGGAGGAGAGCTGCAGCAAGATGGGGATCTTGAAAGCCATTATCATAGTCCATTGCTTGATACAGAGCCAGCTGTTCATGACATTCATGTCATAGATGGTAAAACTGAAATAGTAAGAGGAGAAAGTAGGCCATCAATTTATGCTGCTTTGGATGGATTCCAAGATCCGAGATGTGGGGCTTCTGGAGTCTGTAGCAGCTTGGAACCTCCGCTGAGCATGGGGAAAGGCAAAACGCCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

509

Amino Acids

56.2

Weight (kDa)

5.21

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 248 - 310 4.5e-15 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1373
AccIII TCCGGA 1 cut(s) 605
AciI CCGC 1 cut(s) 1496
AclWI GGATC 3 cut(s) 294, 1320, 1445
AcoI YGGCCR 1 cut(s) 843
AcsI RAATTY 2 cut(s) 471, 946
AcuI CTGAAG 2 cut(s) 476, 1260
AfaI GTAC 2 cut(s) 541, 1105
AfiI CCNNNNNNNGG 2 cut(s) 158, 1460
AgsI TTSAA 8 cut(s) 20, 124, 350, 498, 716, 929, 981, 1319
AjnI CCWGG 1 cut(s) 1225
AloI GAACNNNNNNTCC 2 cut(s) 478, 510
Alw21I GWGCWC 1 cut(s) 754
AlwI GGATC 3 cut(s) 294, 1320, 1445
AlwNI CAGNNNCTG 1 cut(s) 662
Aor13HI TCCGGA 1 cut(s) 605
AoxI GGCC 3 cut(s) 215, 843, 1415
ApoI RAATTY 2 cut(s) 471, 946
ArsI GACNNNNNNTTYG 2 cut(s) 1109, 1141
Asp700I GAANNNNTTC 2 cut(s) 128, 1274
AsuHPI GGTGA 5 cut(s) 262, 371, 569, 704, 986
AsuII TTCGAA 1 cut(s) 1022
BaeGI GKGCMC 1 cut(s) 1226
BalI TGGCCA 1 cut(s) 845
BarI GAAGNNNNNNTAC 2 cut(s) 1185, 1217
BauI CACGAG 1 cut(s) 507
Bbv12I GWGCWC 1 cut(s) 754
BccI CCATC 6 cut(s) 1182, 1280, 1301, 1376, 1426, 1433
BcgI CGANNNNNNTGC 2 cut(s) 1002, 1036
BciT130I CCWGG 1 cut(s) 1227
BciVI GTATCC 1 cut(s) 714
BfaI CTAG 2 cut(s) 483, 774
BfmI CTRYAG 5 cut(s) 573, 860, 1279, 1298, 1477
BfuI GTATCC 1 cut(s) 714
BlpI GCTNAGC 1 cut(s) 1499
Bme1390I CCNGG 1 cut(s) 1227
BmiI GGNNCC 2 cut(s) 263, 1491
BmrFI CCNGG 1 cut(s) 1227
BmsI GCATC 5 cut(s) 418, 611, 670, 884, 1035
BpmI CTGGAG 2 cut(s) 818, 1491
Bpu10I CCTNAGC 1 cut(s) 432
Bpu1102I GCTNAGC 1 cut(s) 1499
Bpu14I TTCGAA 1 cut(s) 1022
BpuEI CTTGAG 2 cut(s) 322, 582
BsaBI GATNNNNATC 1 cut(s) 1311
BsaJI CCNNGG 1 cut(s) 1225
BsaWI WCCGGW 1 cut(s) 605
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 478, 508
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 1460
Bse1I ACTGG 1 cut(s) 472
Bse3DI GCAATG 3 cut(s) 846, 1224, 1337
Bse8I GATNNNNATC 1 cut(s) 1311
BseAI TCCGGA 1 cut(s) 605
BseBI CCWGG 1 cut(s) 1227
BseDI CCNNGG 1 cut(s) 1225
BseGI GGATG 7 cut(s) 328, 535, 602, 685, 744, 1193, 1444
BseJI GATNNNNATC 1 cut(s) 1311
BseLI CCNNNNNNNGG 2 cut(s) 158, 1460
BseMI GCAATG 3 cut(s) 846, 1224, 1337
BseMII CTCAG 3 cut(s) 654, 1124, 1490
BseNI ACTGG 1 cut(s) 472
BseRI GAGGAG 3 cut(s) 495, 1305, 1419
BseSI GKGCMC 1 cut(s) 1226
BsgI GTGCAG 1 cut(s) 996
BshFI GGCC 3 cut(s) 217, 845, 1417
BsiHKAI GWGCWC 1 cut(s) 754
BsiSI CCGG 1 cut(s) 606
BslFI GGGAC 1 cut(s) 1017
BslI CCNNNNNNNGG 2 cut(s) 158, 1460
BsmFI GGGAC 1 cut(s) 1017
BsnI GGCC 3 cut(s) 217, 845, 1417
Bsp119I TTCGAA 1 cut(s) 1022
Bsp1286I GDGCHC 2 cut(s) 754, 1226
Bsp13I TCCGGA 1 cut(s) 605
Bsp143I GATC 4 cut(s) 184, 286, 1312, 1450
Bsp1720I GCTNAGC 1 cut(s) 1499
BspACI CCGC 1 cut(s) 1496
BspANI GGCC 3 cut(s) 217, 845, 1417
BspCNI CTCAG 3 cut(s) 655, 1123, 1491
BspEI TCCGGA 1 cut(s) 605
BspHI TCATGA 1 cut(s) 1363
BspLI GGNNCC 2 cut(s) 263, 1491
BspMAI CTGCAG 2 cut(s) 864, 1302
BspPI GGATC 3 cut(s) 294, 1320, 1445
BspT104I TTCGAA 1 cut(s) 1022
BsrDI GCAATG 3 cut(s) 846, 1224, 1337
BsrI ACTGG 1 cut(s) 472
BssECI CCNNGG 1 cut(s) 1225
BssMI GATC 4 cut(s) 184, 286, 1312, 1450
BssSI CACGAG 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 1227
Bst4CI ACNGT 4 cut(s) 46, 692, 968, 1103
Bst6I CTCTTC 3 cut(s) 248, 434, 885
BstAPI GCANNNNNTGC 3 cut(s) 202, 827, 1214
BstBI TTCGAA 1 cut(s) 1022
BstC8I GCNNGC 3 cut(s) 194, 810, 1356
BstDEI CTNAG 6 cut(s) 432, 642, 663, 1110, 1254, 1499
BstF5I GGATG 7 cut(s) 328, 535, 602, 685, 744, 1193, 1444
BstKTI GATC 4 cut(s) 187, 289, 1315, 1453
BstMBI GATC 4 cut(s) 184, 286, 1312, 1450
BstMWI GCNNNNNNNGC 9 cut(s) 202, 578, 779, 818, 824, 827, 836, 1214, 1250
BstNI CCWGG 1 cut(s) 1227
BstNSI RCATGY 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 1225
BstSFI CTRYAG 5 cut(s) 573, 860, 1279, 1298, 1477
BstSLI GKGCMC 1 cut(s) 1226
BstX2I RGATCY 2 cut(s) 1312, 1450
BstXI CCANNNNNNTGG 1 cut(s) 1151
BstYI RGATCY 2 cut(s) 1312, 1450
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 3 cut(s) 217, 845, 1417
BtsCI GGATG 7 cut(s) 328, 535, 602, 685, 744, 1193, 1444
BtsIMutI CAGTG 1 cut(s) 964
Cac8I GCNNGC 3 cut(s) 194, 810, 1356
CaiI CAGNNNCTG 1 cut(s) 662
CciI TCATGA 1 cut(s) 1363
CseI GACGC 1 cut(s) 1127
Csp6I GTAC 2 cut(s) 540, 1104
CviAII CATG 9 cut(s) 24, 294, 366, 847, 995, 1157, 1364, 1373, 1504
CviQI GTAC 2 cut(s) 540, 1104
DdeI CTNAG 6 cut(s) 432, 642, 663, 1110, 1254, 1499
DpnI GATC 4 cut(s) 186, 288, 1314, 1452
DpnII GATC 4 cut(s) 184, 286, 1312, 1450
DrdI GACNNNNNNGTC 1 cut(s) 1373
DseDI GACNNNNNNGTC 1 cut(s) 1373
EaeI YGGCCR 1 cut(s) 843
Eam1104I CTCTTC 3 cut(s) 248, 434, 885
EarI CTCTTC 3 cut(s) 248, 434, 885
Eco57I CTGAAG 2 cut(s) 476, 1260
EcoRI GAATTC 2 cut(s) 471, 946
EcoRII CCWGG 1 cut(s) 1225
EcoT22I ATGCAT 1 cut(s) 89
FaeI CATG 9 cut(s) 27, 297, 369, 850, 998, 1160, 1367, 1376, 1507
FaqI GGGAC 1 cut(s) 1017
FatI CATG 9 cut(s) 23, 293, 365, 846, 994, 1156, 1363, 1372, 1503
FauNDI CATATG 1 cut(s) 1077
FokI GGATG 7 cut(s) 335, 542, 589, 692, 751, 1200, 1451
FspBI CTAG 2 cut(s) 483, 774
GsuI CTGGAG 2 cut(s) 818, 1491
HaeIII GGCC 3 cut(s) 217, 845, 1417
HapII CCGG 1 cut(s) 606
HgaI GACGC 1 cut(s) 1127
Hin1II CATG 9 cut(s) 27, 297, 369, 850, 998, 1160, 1367, 1376, 1507
HinfI GANTC 9 cut(s) 304, 362, 385, 653, 905, 977, 1031, 1443, 1473
HpaII CCGG 1 cut(s) 606
HphI GGTGA 5 cut(s) 262, 371, 569, 704, 986
Hpy166II GTNNAC 2 cut(s) 635, 1053
Hpy188I TCNGA 4 cut(s) 243, 309, 456, 1455
Hpy8I GTNNAC 2 cut(s) 635, 1053
HpyAV CCTTC 8 cut(s) 118, 350, 617, 740, 859, 893, 913, 1187
HpyCH4III ACNGT 4 cut(s) 46, 692, 968, 1103
HpyF10VI GCNNNNNNNGC 9 cut(s) 202, 578, 779, 818, 824, 827, 836, 1214, 1250
HpyF3I CTNAG 6 cut(s) 432, 642, 663, 1110, 1254, 1499
Hsp92II CATG 9 cut(s) 27, 297, 369, 850, 998, 1160, 1367, 1376, 1507
Kpn2I TCCGGA 1 cut(s) 605
Kzo9I GATC 4 cut(s) 184, 286, 1312, 1450
LmnI GCTCC 2 cut(s) 27, 749
LweI GCATC 5 cut(s) 418, 611, 670, 884, 1035
MaeI CTAG 2 cut(s) 483, 774
MaeIII GTNAC 1 cut(s) 686
MalI GATC 4 cut(s) 186, 288, 1314, 1452
MboI GATC 4 cut(s) 184, 286, 1312, 1450
MboII GAAGA 6 cut(s) 265, 451, 469, 707, 902, 1011
MfeI CAATTG 1 cut(s) 567
MflI RGATCY 2 cut(s) 1312, 1450
MhlI GDGCHC 2 cut(s) 754, 1226
MlsI TGGCCA 1 cut(s) 845
MluCI AATT 6 cut(s) 350, 444, 471, 567, 946, 1422
MluNI TGGCCA 1 cut(s) 845
MlyI GAGTC 4 cut(s) 313, 371, 394, 1482
MmeI TCCRAC 1 cut(s) 723
Mox20I TGGCCA 1 cut(s) 845
Mph1103I ATGCAT 1 cut(s) 89
MroI TCCGGA 1 cut(s) 605
MroXI GAANNNNTTC 2 cut(s) 128, 1274
MscI TGGCCA 1 cut(s) 845
MseI TTAA 3 cut(s) 207, 282, 1528
MslI CAYNNNNRTG 2 cut(s) 969, 1149
Msp20I TGGCCA 1 cut(s) 845
MspA1I CMGCKG 3 cut(s) 659, 1358, 1498
MspI CCGG 1 cut(s) 606
MspR9I CCNGG 1 cut(s) 1227
MunI CAATTG 1 cut(s) 567
MvaI CCWGG 1 cut(s) 1227
MwoI GCNNNNNNNGC 9 cut(s) 202, 578, 779, 818, 824, 827, 836, 1214, 1250
NdeI CATATG 1 cut(s) 1077
NdeII GATC 4 cut(s) 184, 286, 1312, 1450
NlaIII CATG 9 cut(s) 27, 297, 369, 850, 998, 1160, 1367, 1376, 1507
NlaIV GGNNCC 2 cut(s) 263, 1491
NsiI ATGCAT 1 cut(s) 89
NspI RCATGY 1 cut(s) 297
NspV TTCGAA 1 cut(s) 1022
PagI TCATGA 1 cut(s) 1363
PdmI GAANNNNTTC 2 cut(s) 128, 1274
PfeI GAWTC 5 cut(s) 653, 905, 977, 1031, 1443
PleI GAGTC 4 cut(s) 312, 370, 393, 1481
PpsI GAGTC 4 cut(s) 312, 370, 393, 1481
Psp6I CCWGG 1 cut(s) 1225
PspGI CCWGG 1 cut(s) 1225
PspN4I GGNNCC 2 cut(s) 263, 1491
PstI CTGCAG 2 cut(s) 864, 1302
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 2 cut(s) 1312, 1450
PvuII CAGCTG 2 cut(s) 659, 1358
RsaI GTAC 2 cut(s) 541, 1105
RsaNI GTAC 2 cut(s) 540, 1104
RseI CAYNNNNRTG 2 cut(s) 969, 1149
SaqAI TTAA 3 cut(s) 207, 282, 1528
Sau3AI GATC 4 cut(s) 184, 286, 1312, 1450
SchI GAGTC 4 cut(s) 313, 371, 394, 1482
ScrFI CCNGG 1 cut(s) 1227
SduI GDGCHC 2 cut(s) 754, 1226
SfaNI GCATC 5 cut(s) 418, 611, 670, 884, 1035
SfcI CTRYAG 5 cut(s) 573, 860, 1279, 1298, 1477
SfuI TTCGAA 1 cut(s) 1022
SmiMI CAYNNNNRTG 2 cut(s) 969, 1149
SmlI CTYRAG 2 cut(s) 301, 597
SmoI CTYRAG 2 cut(s) 301, 597
Sse9I AATT 6 cut(s) 350, 444, 471, 567, 946, 1422
SsiI CCGC 1 cut(s) 1496
SspI AATATT 1 cut(s) 1093
SspMI CTAG 2 cut(s) 483, 774
StyD4I CCNGG 1 cut(s) 1225
TaaI ACNGT 4 cut(s) 46, 692, 968, 1103
TaqI TCGA 5 cut(s) 113, 383, 756, 878, 1022
TasI AATT 6 cut(s) 350, 444, 471, 567, 946, 1422
TfiI GAWTC 5 cut(s) 653, 905, 977, 1031, 1443
Tru1I TTAA 3 cut(s) 207, 282, 1528
Tru9I TTAA 3 cut(s) 207, 282, 1528
TscAI CASTG 1 cut(s) 971
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 1 cut(s) 971
XapI RAATTY 2 cut(s) 471, 946
XceI RCATGY 1 cut(s) 297
XmnI GAANNNNTTC 2 cut(s) 128, 1274
XspI CTAG 2 cut(s) 483, 774
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.