FvH4_2g19000

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008250
Physical Location & Seq
Reverse (-)
16159087 .. 16163418
4332 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19000.t6

Sequence Viewer

Length: 1614 bp
ATGGCTTGTGAGGAAGTTCAATCATGGAGCTTTGGTGGTCTTACAGGAGCATTTCTTGACCTTTTAGTAGCTTACTTCCTGCTGTGCATATCAGCTTGTGTGTTTTTTGTGTCCAAATTGTTGAATTTTTGCTGGGTGTATTTGCCTTGTCCTTGTAATGGGGTTTTCGGGTTTAGAAACCGAGATCTTTGCTTGCATAAGTTGCTTAATGAGTGGCCTGTTGCGAAGATTTGTGCTGTTCAGAAGCTGGTGATGAGTAGGTTCCCTTTTGATATTCGGTTTAAGGATCAGACAAGCAATTTGAGTCAGAAGTTGATTAGGGATGTGAATGGAGAGAATGGTGTTCTTGAATTCGGAGGTGAGTCGTGTTATAGTCCGTTTTCGAGTCCAAGATTGCAAAGCTTGGTTGATAAGGAAAGTGGGTATGATGCTAAGGGGAAAAGAATTGTTGTTCTGAAGAAGAGGACTGGTATTCGGCGGCCTAGGAGGGATAGTTCAAAATCTCCTCGTATATTCCCCTTGCCTCTTGATGAAAGAGAAACAAGAGAGGTATCTGGTGAAAGCTCAATTGCTCTAGCTGCAAGACAGGATGAGCCTCAAGCAACCGGAGATGATACAGGGGAAAGAATACATGGAAATTCCAAGTTTAGTGGATCAGCAGTTGAGAGTAAAGGTCTGGACATAAGTTACGGTGAAGAAAAGTTTCCCGTTGTTGAAAATGAAAGTGATACAATCAGGATTTTGCAAGGAGCACTCGCGAAAGAGAAAGCTGCCACTGCTGCTCTCTATTTAGAGCTGGAGAAAGAGCGAGCTGCTGCTGCAACTGCTGCTGATGAAGCAATGGCCATGATATATCGTTTGCAGAAGGATAAAGCATCAACAGAAATGGAAGTGAGACAGTACCAAAGGATGATTGAAGAGAAATTTGTTTATGATGAAGAAGAAATGGATGTTCTGAAAGAGATACTTCTTACGCGGGAGAAGGAGAATCACTTTTTGGAGAAGGAAGTTGAAGCATATAGACAGATGAATTCCTTAGGAAGTGAACAGTCTTATGGTGATTCAAATGATAAGTTGTGTGAATGGAGACAAACTGCAGTTTCTTCGAATATAGATTCACAACTGATGATGCCGTGGACTAATGAAACAAAATCTAACCATATGGGAGTGGTGAATATTGCAGACAGTACCTCTCAGTTTGAGGCTTCATTTGTTGAAAAACAAATTCATTCCAATGGACATGATGTTGTTGAGAAAAGTGTCCTTTCAGCATGGGAAGAAAAACTAGAAACAGATAATGCAATGTGCCCAGGAATGACAACTGAATCACTCCAAGCTAATATTGGGATCAACAAACAATTCTATTGTGATGGAGAAGAGCGGCAGCAAAATGGGAACACTAAAAACAATTGTCAAAGTCCGATGCTTGATACAGAGACAGCTGTTTATGATGTTCATGTCATAGAAGGTAAAGATGAAATGTTGAAAGGAGAAAGTAGGCCATCAATGTATGCTGCTTTGGAGGGACTCCAAGATTTGACATGTGCAGCATCTGGGGTCTGTAGCAGCTCGGAACCTCTGCTGAGCGTAGGAAAAGGCAAAACTCCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

538

Amino Acids

60.05

Weight (kDa)

5.01

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 69 - 158 2e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1381
AccII CGCG 2 cut(s) 758, 976
AciI CCGC 3 cut(s) 478, 976, 1381
AclWI GGATC 3 cut(s) 294, 661, 1355
AcoI YGGCCR 1 cut(s) 843
AcsI RAATTY 6 cut(s) 124, 350, 637, 923, 1030, 1224
AcuI CTGAAG 1 cut(s) 476
AfaI GTAC 2 cut(s) 902, 1189
AfiI CCNNNNNNNGG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 1541
AjnI CCWGG 1 cut(s) 1309
AjuI GAANNNNNNNTTGG 2 cut(s) 980, 1012
AloI GAACNNNNNNTCC 2 cut(s) 478, 510
Alw21I GWGCWC 1 cut(s) 754
Alw26I GTCTC 3 cut(s) 889, 1081, 1430
AlwI GGATC 3 cut(s) 294, 661, 1355
AlwNI CAGNNNCTG 2 cut(s) 247, 1553
AoxI GGCC 4 cut(s) 215, 479, 843, 1499
ApoI RAATTY 6 cut(s) 124, 350, 637, 923, 1030, 1224
ArsI GACNNNNNNTTYG 2 cut(s) 283, 315
Asp700I GAANNNNTTC 1 cut(s) 702
AspA2I CCTAGG 1 cut(s) 482
AsuHPI GGTGA 6 cut(s) 262, 371, 569, 704, 1070, 1183
AsuII TTCGAA 1 cut(s) 1106
AvrII CCTAGG 1 cut(s) 482
AxyI CCTNAGG 1 cut(s) 1036
BaeGI GKGCMC 1 cut(s) 1310
BalI TGGCCA 1 cut(s) 845
Bbv12I GWGCWC 1 cut(s) 754
BccI CCATC 2 cut(s) 1364, 1510
BceAI ACGGC 1 cut(s) 1117
BcgI CGANNNNNNTGC 4 cut(s) 171, 205, 1086, 1120
BciT130I CCWGG 1 cut(s) 1311
BcoDI GTCTC 3 cut(s) 889, 1081, 1430
BfaI CTAG 3 cut(s) 483, 575, 1286
BfmI CTRYAG 2 cut(s) 1095, 1561
BglII AGATCT 1 cut(s) 184
BlnI CCTAGG 1 cut(s) 482
BlpI GCTNAGC 1 cut(s) 1583
Bme1390I CCNGG 1 cut(s) 1311
BmiI GGNNCC 2 cut(s) 263, 1575
BmrFI CCNGG 1 cut(s) 1311
BmsI GCATC 5 cut(s) 418, 884, 1119, 1413, 1559
BpmI CTGGAG 1 cut(s) 818
Bpu10I CCTNAGC 1 cut(s) 432
Bpu1102I GCTNAGC 1 cut(s) 1583
Bpu14I TTCGAA 1 cut(s) 1106
BpuEI CTTGAG 1 cut(s) 582
BsaJI CCNNGG 3 cut(s) 482, 1133, 1309
BsaWI WCCGGW 1 cut(s) 605
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 478, 508
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse1I ACTGG 1 cut(s) 472
Bse21I CCTNAGG 1 cut(s) 1036
Bse3DI GCAATG 2 cut(s) 846, 1308
BseBI CCWGG 1 cut(s) 1311
BseDI CCNNGG 3 cut(s) 482, 1133, 1309
BseGI GGATG 4 cut(s) 328, 595, 915, 955
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMI GCAATG 2 cut(s) 846, 1308
BseMII CTCAG 2 cut(s) 1208, 1574
BseNI ACTGG 1 cut(s) 472
BseRI GAGGAG 2 cut(s) 495, 1596
BseSI GKGCMC 1 cut(s) 1310
BseYI CCCAGC 1 cut(s) 132
BsgI GTGCAG 1 cut(s) 1566
Bsh1236I CGCG 2 cut(s) 758, 976
BshFI GGCC 4 cut(s) 217, 481, 845, 1501
BsiHKAI GWGCWC 1 cut(s) 754
BsiSI CCGG 1 cut(s) 606
BslFI GGGAC 1 cut(s) 1539
BslI CCNNNNNNNGG 1 cut(s) 158
BsmAI GTCTC 3 cut(s) 889, 1081, 1430
BsmFI GGGAC 1 cut(s) 1539
BsnI GGCC 4 cut(s) 217, 481, 845, 1501
Bsp119I TTCGAA 1 cut(s) 1106
Bsp1286I GDGCHC 2 cut(s) 754, 1310
Bsp143I GATC 4 cut(s) 184, 286, 653, 1347
Bsp1720I GCTNAGC 1 cut(s) 1583
Bsp68I TCGCGA 1 cut(s) 758
BspACI CCGC 3 cut(s) 478, 976, 1381
BspANI GGCC 4 cut(s) 217, 481, 845, 1501
BspCNI CTCAG 2 cut(s) 1207, 1575
BspFNI CGCG 2 cut(s) 758, 976
BspLI GGNNCC 2 cut(s) 263, 1575
BspMAI CTGCAG 1 cut(s) 1099
BspPI GGATC 3 cut(s) 294, 661, 1355
BspQI GCTCTTC 1 cut(s) 1371
BspT104I TTCGAA 1 cut(s) 1106
BsrBI CCGCTC 1 cut(s) 1381
BsrDI GCAATG 2 cut(s) 846, 1308
BsrI ACTGG 1 cut(s) 472
BssECI CCNNGG 3 cut(s) 482, 1133, 1309
BssMI GATC 4 cut(s) 184, 286, 653, 1347
BssT1I CCWWGG 1 cut(s) 482
Bst2UI CCWGG 1 cut(s) 1311
Bst4CI ACNGT 4 cut(s) 692, 900, 1050, 1187
Bst6I CTCTTC 3 cut(s) 455, 912, 1371
BstAPI GCANNNNNTGC 2 cut(s) 202, 827
BstBI TTCGAA 1 cut(s) 1106
BstC8I GCNNGC 2 cut(s) 194, 810
BstDEI CTNAG 4 cut(s) 432, 1036, 1194, 1583
BstDSI CCRYGG 1 cut(s) 1133
BstF5I GGATG 4 cut(s) 328, 595, 915, 955
BstFNI CGCG 2 cut(s) 758, 976
BstKTI GATC 4 cut(s) 187, 289, 656, 1350
BstMAI GTCTC 3 cut(s) 889, 1081, 1430
BstMBI GATC 4 cut(s) 184, 286, 653, 1347
BstMWI GCNNNNNNNGC 8 cut(s) 202, 578, 776, 779, 818, 824, 827, 836
BstNI CCWGG 1 cut(s) 1311
BstNSI RCATGY 1 cut(s) 1545
BstSCI CCNGG 1 cut(s) 1309
BstSFI CTRYAG 2 cut(s) 1095, 1561
BstSLI GKGCMC 1 cut(s) 1310
BstUI CGCG 2 cut(s) 758, 976
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
Bsu36I CCTNAGG 1 cut(s) 1036
BsuRI GGCC 4 cut(s) 217, 481, 845, 1501
BtgI CCRYGG 1 cut(s) 1133
BtsCI GGATG 4 cut(s) 328, 595, 915, 955
BtsI GCAGTG 1 cut(s) 774
BtsIMutI CAGTG 1 cut(s) 774
BtuMI TCGCGA 1 cut(s) 758
Cac8I GCNNGC 2 cut(s) 194, 810
CaiI CAGNNNCTG 2 cut(s) 247, 1553
Csp6I GTAC 2 cut(s) 901, 1188
CspCI CAANNNNNGTGG 2 cut(s) 631, 666
CviAII CATG 7 cut(s) 24, 632, 847, 1241, 1272, 1457, 1542
CviQI GTAC 2 cut(s) 901, 1188
DdeI CTNAG 4 cut(s) 432, 1036, 1194, 1583
DpnI GATC 4 cut(s) 186, 288, 655, 1349
DpnII GATC 4 cut(s) 184, 286, 653, 1347
EaeI YGGCCR 1 cut(s) 843
Eam1104I CTCTTC 3 cut(s) 455, 912, 1371
EarI CTCTTC 3 cut(s) 455, 912, 1371
Eco130I CCWWGG 1 cut(s) 482
Eco57I CTGAAG 1 cut(s) 476
Eco81I CCTNAGG 1 cut(s) 1036
EcoRI GAATTC 2 cut(s) 350, 1030
EcoRII CCWGG 1 cut(s) 1309
EcoT14I CCWWGG 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 482
FaeI CATG 7 cut(s) 27, 635, 850, 1244, 1275, 1460, 1545
FalI AAGNNNNNCTT 2 cut(s) 951, 983
FaqI GGGAC 1 cut(s) 1539
FatI CATG 7 cut(s) 23, 631, 846, 1240, 1271, 1456, 1541
FauI CCCGC 1 cut(s) 969
FauNDI CATATG 1 cut(s) 1161
FokI GGATG 4 cut(s) 335, 602, 922, 962
FspBI CTAG 3 cut(s) 483, 575, 1286
GsaI CCCAGC 1 cut(s) 136
GsuI CTGGAG 1 cut(s) 818
HaeIII GGCC 4 cut(s) 217, 481, 845, 1501
HapII CCGG 1 cut(s) 606
Hin1II CATG 7 cut(s) 27, 635, 850, 1244, 1275, 1460, 1545
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 8 cut(s) 304, 362, 385, 988, 1061, 1115, 1325, 1527
HpaII CCGG 1 cut(s) 606
HphI GGTGA 6 cut(s) 262, 371, 569, 704, 1070, 1183
Hpy166II GTNNAC 2 cut(s) 1046, 1137
Hpy188I TCNGA 8 cut(s) 243, 291, 309, 356, 456, 957, 1422, 1573
Hpy188III TCNNGA 6 cut(s) 56, 347, 527, 677, 736, 757
Hpy8I GTNNAC 2 cut(s) 1046, 1137
HpyAV CCTTC 4 cut(s) 859, 976, 997, 1460
HpyCH4III ACNGT 4 cut(s) 692, 900, 1050, 1187
HpyF10VI GCNNNNNNNGC 8 cut(s) 202, 578, 776, 779, 818, 824, 827, 836
HpyF3I CTNAG 4 cut(s) 432, 1036, 1194, 1583
Hsp92II CATG 7 cut(s) 27, 635, 850, 1244, 1275, 1460, 1545
Kzo9I GATC 4 cut(s) 184, 286, 653, 1347
LguI GCTCTTC 1 cut(s) 1371
LmnI GCTCC 3 cut(s) 27, 47, 749
LweI GCATC 5 cut(s) 418, 884, 1119, 1413, 1559
MaeI CTAG 3 cut(s) 483, 575, 1286
MaeIII GTNAC 1 cut(s) 686
MalI GATC 4 cut(s) 186, 288, 655, 1349
MbiI CCGCTC 1 cut(s) 1381
MboI GATC 4 cut(s) 184, 286, 653, 1347
MfeI CAATTG 2 cut(s) 567, 1408
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 2 cut(s) 754, 1310
MlsI TGGCCA 1 cut(s) 845
MluNI TGGCCA 1 cut(s) 845
MlyI GAGTC 4 cut(s) 313, 371, 394, 1521
Mox20I TGGCCA 1 cut(s) 845
MroXI GAANNNNTTC 1 cut(s) 702
MscI TGGCCA 1 cut(s) 845
MseI TTAA 2 cut(s) 207, 282
MslI CAYNNNNRTG 1 cut(s) 1233
Msp20I TGGCCA 1 cut(s) 845
MspA1I CMGCKG 1 cut(s) 1442
MspI CCGG 1 cut(s) 606
MspR9I CCNGG 1 cut(s) 1311
MunI CAATTG 2 cut(s) 567, 1408
MvaI CCWGG 1 cut(s) 1311
MvnI CGCG 2 cut(s) 758, 976
MwoI GCNNNNNNNGC 8 cut(s) 202, 578, 776, 779, 818, 824, 827, 836
NdeI CATATG 1 cut(s) 1161
NdeII GATC 4 cut(s) 184, 286, 653, 1347
NlaIII CATG 7 cut(s) 27, 635, 850, 1244, 1275, 1460, 1545
NlaIV GGNNCC 2 cut(s) 263, 1575
NruI TCGCGA 1 cut(s) 758
NspI RCATGY 1 cut(s) 1545
NspV TTCGAA 1 cut(s) 1106
PciI ACATGT 1 cut(s) 1541
PciSI GCTCTTC 1 cut(s) 1371
PdmI GAANNNNTTC 1 cut(s) 702
PfeI GAWTC 4 cut(s) 988, 1061, 1115, 1325
PleI GAGTC 4 cut(s) 312, 370, 393, 1521
PpsI GAGTC 4 cut(s) 312, 370, 393, 1521
PscI ACATGT 1 cut(s) 1541
Psp6I CCWGG 1 cut(s) 1309
PspFI CCCAGC 1 cut(s) 132
PspGI CCWGG 1 cut(s) 1309
PspN4I GGNNCC 2 cut(s) 263, 1575
PstI CTGCAG 1 cut(s) 1099
PstNI CAGNNNCTG 2 cut(s) 247, 1553
PsuI RGATCY 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 1442
RruI TCGCGA 1 cut(s) 758
RsaI GTAC 2 cut(s) 902, 1189
RsaNI GTAC 2 cut(s) 901, 1188
RseI CAYNNNNRTG 1 cut(s) 1233
SapI GCTCTTC 1 cut(s) 1371
SaqAI TTAA 2 cut(s) 207, 282
Sau3AI GATC 4 cut(s) 184, 286, 653, 1347
SchI GAGTC 4 cut(s) 313, 371, 394, 1521
ScrFI CCNGG 1 cut(s) 1311
SduI GDGCHC 2 cut(s) 754, 1310
SfaNI GCATC 5 cut(s) 418, 884, 1119, 1413, 1559
SfcI CTRYAG 2 cut(s) 1095, 1561
SfuI TTCGAA 1 cut(s) 1106
SmiMI CAYNNNNRTG 1 cut(s) 1233
SmlI CTYRAG 1 cut(s) 597
SmoI CTYRAG 1 cut(s) 597
SsiI CCGC 3 cut(s) 478, 976, 1381
SspI AATATT 2 cut(s) 1177, 1342
SspMI CTAG 3 cut(s) 483, 575, 1286
StyD4I CCNGG 1 cut(s) 1309
StyI CCWWGG 1 cut(s) 482
TaaI ACNGT 4 cut(s) 692, 900, 1050, 1187
TaqI TCGA 2 cut(s) 383, 1106
TauI GCSGC 2 cut(s) 481, 1384
TfiI GAWTC 4 cut(s) 988, 1061, 1115, 1325
Tru1I TTAA 2 cut(s) 207, 282
Tru9I TTAA 2 cut(s) 207, 282
TscAI CASTG 1 cut(s) 781
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 1 cut(s) 781
XapI RAATTY 6 cut(s) 124, 350, 637, 923, 1030, 1224
XceI RCATGY 1 cut(s) 1545
XcmI CCANNNNNNNNNTGG 1 cut(s) 1340
XmaJI CCTAGG 1 cut(s) 482
XmnI GAANNNNTTC 1 cut(s) 702
XspI CTAG 3 cut(s) 483, 575, 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.