Rh6AG279500

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0008250
Physical Location & Seq
Reverse (-)
47416449 .. 47419475
3027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG279500.1

Sequence Viewer

Length: 1548 bp
ATGGCTTGTCAGGAAGTTCAATCATGGAGCTTTGGTGGTCTTACAGTAGCATTTCTTGACCTTTTACTTGCTTATTTCCTGCTATGCATATCAGCTTTTGTGTTTTTCGTATCGAAGTTGTTGAAGGTTTTCTGGGTGTATTTGCCTTGTCCTTGTAATGGGGTTTTCGGGTATAGAAATCGTGATCTTTGCTTGCATAAGTTGCTTAATGAGTGGCCTGTTGCCAAGATTTATGCTGTTCAGAATTTGGTGAAGAGTAGGTTCCCTTTTGATATTCGGTTGAAGGATCAAACATGCAACTTGAGTCAGAAGTTGATTAGGGATGTGAATGGAGGGAATGGTGTTCTTGAATTGGAAGGTGAGTCATGTTGTAGTCCGTTTTCGAGCCCGAGATTGCAAAGTCTGGTTGATAAGGAAAGTGGGTATGATGCTAAGGGGAAGAGAATTGTGATTCTGAAGAAAAGGACTGGAATTCGGCGTTCTAGGAGGGATAGTTCAAAATCTCCTCGTATATTCCCCTTTCCTCTTGATGACAGAGGTACAAGAGAGATATCTGGTGAAAGCTCAATTGCTGTAGCTGCAAGACACGATGAGCCTCAAGCATCCGGAGACGATAGAGGGGAAGGAATATGTGGACTTTCTAAGTTGAGTGAATCAGCTGCTGAGAGTAAAGGTCTGGATGCAAGTTATGGTGAAGAAAAGTTTCCCATTTTTGAAAATGAAAGTGATACAATCAGGATGTTGGAAGGAGCACTCGAAAAAGAGAAAGCTGCTTGTGCTGCTCTCTATTTAGAGCTGGAGGAAGAGCGAGCTGCTGCTGCAACTGCTGCTGATGAAGCAATGGCCATGATAAGTCGTCTGCAGAAGGATAAAGCATTGACAGAAATGGAAGTGAGACAGTACCAAAGGATGATTGAAGAGAAATTTGTTTATGATGAAGAAGAGATGGATGTTCTGAAAGAGATACTTCTTAGGAGGGAGAAGGAGAATCATTATTTGGAGAAGGAAGTTGAAGCATATAGACAGATGAATTCTTTAGGAAATGAACAGTGTAATGGTGATTCAAATGATAGGCTGCGTGAATGGGGACAAACTGCACTTTCTTCGAATATAGATTCACAACTGATGATGCCGTGGACTAATGAAACTAAATCTAATCATATGGAAGTGGGAAATATTGCAGACAGTACCTCTCAGTTTGACGCTTCATTTGTCAAAAAACAAATCCATTCCAATGGACATGATGTTGTTGAGAAAAGTGTCCTTTCAGGATGGGAAGGAAAACAATACGCAGATAATGCAATGTGCCCAGGAATGACTACTGAAGCAGTCCAAGCTAAGATAGGGATTGAGAAAAGTTTCTATAGTGATGGAGGAGAGCTGCAGCAAGGTGGGCATCTTGAAAGCCATTATCATAGTCCATTGCTTGATACAGAGCCAGCTGTTCATGACATTCATGTCATAGATGGTAAAACTGAAATAGTAAGAGGAGAAAAGATCGAATGTGACTTGCTTCTTTACCGGCTTTATCTTCTAAGGTGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

515

Amino Acids

58.06

Weight (kDa)

5.21

Isoelectric Point (pI)

49.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 248 - 337 2e-30 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1457
AccIII TCCGGA 1 cut(s) 605
AclWI GGATC 1 cut(s) 294
AcoI YGGCCR 1 cut(s) 843
AcsI RAATTY 4 cut(s) 244, 471, 923, 1030
AcuI CTGAAG 2 cut(s) 476, 1344
AfaI GTAC 3 cut(s) 541, 902, 1189
AfiI CCNNNNNNNGG 1 cut(s) 158
AjnI CCWGG 1 cut(s) 1309
AjuI GAANNNNNNNTTGG 2 cut(s) 980, 1012
AloI GAACNNNNNNTCC 2 cut(s) 478, 510
Alw21I GWGCWC 1 cut(s) 754
Alw26I GTCTC 2 cut(s) 603, 889
AlwI GGATC 1 cut(s) 294
AlwNI CAGNNNCTG 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 388
Aor13HI TCCGGA 1 cut(s) 605
AoxI GGCC 2 cut(s) 215, 843
ApoI RAATTY 4 cut(s) 244, 471, 923, 1030
ArsI GACNNNNNNTTYG 2 cut(s) 1193, 1225
Asp700I GAANNNNTTC 3 cut(s) 128, 702, 1358
AsuHPI GGTGA 5 cut(s) 262, 371, 569, 704, 1070
AsuII TTCGAA 1 cut(s) 1106
AvaI CYCGRG 1 cut(s) 388
BaeGI GKGCMC 1 cut(s) 1310
BalI TGGCCA 1 cut(s) 845
BanII GRGCYC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 754
BccI CCATC 4 cut(s) 940, 1266, 1364, 1460
BceAI ACGGC 1 cut(s) 1117
BcgI CGANNNNNNTGC 2 cut(s) 1086, 1120
BciT130I CCWGG 1 cut(s) 1311
BcoDI GTCTC 2 cut(s) 603, 889
BfaI CTAG 1 cut(s) 483
BfmI CTRYAG 4 cut(s) 573, 860, 1363, 1382
Bme1390I CCNGG 1 cut(s) 1311
BmeT110I CYCGRG 1 cut(s) 388
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 1 cut(s) 1311
BmsI GCATC 5 cut(s) 418, 611, 670, 1119, 1405
BpmI CTGGAG 1 cut(s) 818
Bpu10I CCTNAGC 1 cut(s) 432
Bpu14I TTCGAA 1 cut(s) 1106
BpuEI CTTGAG 2 cut(s) 322, 582
BsaJI CCNNGG 2 cut(s) 1133, 1309
BsaWI WCCGGW 1 cut(s) 605
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 478, 508
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse118I RCCGGY 1 cut(s) 1521
Bse1I ACTGG 1 cut(s) 472
Bse3DI GCAATG 3 cut(s) 846, 1308, 1421
BseAI TCCGGA 1 cut(s) 605
BseBI CCWGG 1 cut(s) 1311
BseDI CCNNGG 2 cut(s) 1133, 1309
BseGI GGATG 7 cut(s) 328, 602, 685, 744, 915, 955, 1277
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMI GCAATG 3 cut(s) 846, 1308, 1421
BseMII CTCAG 2 cut(s) 654, 1208
BseNI ACTGG 1 cut(s) 472
BseRI GAGGAG 3 cut(s) 495, 1389, 1503
BseSI GKGCMC 1 cut(s) 1310
BsgI GTGCAG 1 cut(s) 1080
BshFI GGCC 2 cut(s) 217, 845
BsiHKAI GWGCWC 1 cut(s) 754
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 2 cut(s) 606, 1522
BslFI GGGAC 1 cut(s) 1101
BslI CCNNNNNNNGG 1 cut(s) 158
BsmAI GTCTC 2 cut(s) 603, 889
BsmBI CGTCTC 1 cut(s) 603
BsmFI GGGAC 1 cut(s) 1101
BsnI GGCC 2 cut(s) 217, 845
BsoBI CYCGRG 1 cut(s) 388
Bsp119I TTCGAA 1 cut(s) 1106
Bsp1286I GDGCHC 3 cut(s) 389, 754, 1310
Bsp13I TCCGGA 1 cut(s) 605
Bsp143I GATC 3 cut(s) 184, 286, 1497
BspANI GGCC 2 cut(s) 217, 845
BspCNI CTCAG 2 cut(s) 655, 1207
BspEI TCCGGA 1 cut(s) 605
BspHI TCATGA 1 cut(s) 1447
BspLI GGNNCC 1 cut(s) 263
BspMAI CTGCAG 2 cut(s) 864, 1386
BspPI GGATC 1 cut(s) 294
BspQI GCTCTTC 1 cut(s) 798
BspT104I TTCGAA 1 cut(s) 1106
BsrDI GCAATG 3 cut(s) 846, 1308, 1421
BsrFI RCCGGY 1 cut(s) 1521
BsrI ACTGG 1 cut(s) 472
BssAI RCCGGY 1 cut(s) 1521
BssECI CCNNGG 2 cut(s) 1133, 1309
BssMI GATC 3 cut(s) 184, 286, 1497
Bst2UI CCWGG 1 cut(s) 1311
Bst4CI ACNGT 4 cut(s) 46, 900, 1050, 1187
Bst6I CTCTTC 5 cut(s) 248, 434, 798, 912, 936
BstAPI GCANNNNNTGC 3 cut(s) 202, 827, 1298
BstBI TTCGAA 1 cut(s) 1106
BstC8I GCNNGC 3 cut(s) 194, 810, 1440
BstDEI CTNAG 7 cut(s) 432, 642, 663, 971, 1194, 1338, 1535
BstDSI CCRYGG 1 cut(s) 1133
BstF5I GGATG 7 cut(s) 328, 602, 685, 744, 915, 955, 1277
BstKTI GATC 3 cut(s) 187, 289, 1500
BstMAI GTCTC 2 cut(s) 603, 889
BstMBI GATC 3 cut(s) 184, 286, 1497
BstNI CCWGG 1 cut(s) 1311
BstNSI RCATGY 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 1309
BstSFI CTRYAG 4 cut(s) 573, 860, 1363, 1382
BstSLI GKGCMC 1 cut(s) 1310
BstXI CCANNNNNNTGG 1 cut(s) 1235
BsuRI GGCC 2 cut(s) 217, 845
BtgI CCRYGG 1 cut(s) 1133
BtsCI GGATG 7 cut(s) 328, 602, 685, 744, 915, 955, 1277
BtsIMutI CAGTG 1 cut(s) 1055
Cac8I GCNNGC 3 cut(s) 194, 810, 1440
CaiI CAGNNNCTG 1 cut(s) 662
CciI TCATGA 1 cut(s) 1447
Cfr10I RCCGGY 1 cut(s) 1521
CseI GACGC 1 cut(s) 1211
Csp6I GTAC 3 cut(s) 540, 901, 1188
CviAII CATG 7 cut(s) 24, 294, 366, 847, 1241, 1448, 1457
CviQI GTAC 3 cut(s) 540, 901, 1188
DdeI CTNAG 7 cut(s) 432, 642, 663, 971, 1194, 1338, 1535
DpnI GATC 3 cut(s) 186, 288, 1499
DpnII GATC 3 cut(s) 184, 286, 1497
DrdI GACNNNNNNGTC 1 cut(s) 1457
DseDI GACNNNNNNGTC 1 cut(s) 1457
EaeI YGGCCR 1 cut(s) 843
Eam1104I CTCTTC 5 cut(s) 248, 434, 798, 912, 936
EarI CTCTTC 5 cut(s) 248, 434, 798, 912, 936
Eco24I GRGCYC 1 cut(s) 389
Eco32I GATATC 1 cut(s) 552
Eco57I CTGAAG 2 cut(s) 476, 1344
Eco88I CYCGRG 1 cut(s) 388
EcoRI GAATTC 2 cut(s) 471, 1030
EcoRII CCWGG 1 cut(s) 1309
EcoRV GATATC 1 cut(s) 552
EcoT22I ATGCAT 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 389
Esp3I CGTCTC 1 cut(s) 603
FaeI CATG 7 cut(s) 27, 297, 369, 850, 1244, 1451, 1460
FalI AAGNNNNNCTT 2 cut(s) 951, 983
FaqI GGGAC 1 cut(s) 1101
FatI CATG 7 cut(s) 23, 293, 365, 846, 1240, 1447, 1456
FauNDI CATATG 1 cut(s) 1161
FokI GGATG 7 cut(s) 335, 589, 692, 751, 922, 962, 1284
FriOI GRGCYC 1 cut(s) 389
FspBI CTAG 1 cut(s) 483
GsuI CTGGAG 1 cut(s) 818
HaeIII GGCC 2 cut(s) 217, 845
HapII CCGG 2 cut(s) 606, 1522
HgaI GACGC 1 cut(s) 1211
Hin1II CATG 7 cut(s) 27, 297, 369, 850, 1244, 1451, 1460
HinfI GANTC 7 cut(s) 304, 362, 451, 653, 988, 1061, 1115
HpaII CCGG 2 cut(s) 606, 1522
HphI GGTGA 5 cut(s) 262, 371, 569, 704, 1070
Hpy166II GTNNAC 2 cut(s) 635, 1137
Hpy188I TCNGA 4 cut(s) 243, 309, 456, 957
Hpy8I GTNNAC 2 cut(s) 635, 1137
HpyAV CCTTC 9 cut(s) 118, 277, 350, 617, 740, 859, 976, 997, 1271
HpyCH4III ACNGT 4 cut(s) 46, 900, 1050, 1187
HpyF3I CTNAG 7 cut(s) 432, 642, 663, 971, 1194, 1338, 1535
Hsp92II CATG 7 cut(s) 27, 297, 369, 850, 1244, 1451, 1460
Kpn2I TCCGGA 1 cut(s) 605
Kzo9I GATC 3 cut(s) 184, 286, 1497
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 2 cut(s) 27, 749
LweI GCATC 5 cut(s) 418, 611, 670, 1119, 1405
MaeI CTAG 1 cut(s) 483
MaeIII GTNAC 1 cut(s) 1505
MalI GATC 3 cut(s) 186, 288, 1499
MboI GATC 3 cut(s) 184, 286, 1497
MfeI CAATTG 1 cut(s) 567
MhlI GDGCHC 3 cut(s) 389, 754, 1310
MlsI TGGCCA 1 cut(s) 845
MluCI AATT 7 cut(s) 244, 350, 444, 471, 567, 923, 1030
MluNI TGGCCA 1 cut(s) 845
MlyI GAGTC 2 cut(s) 313, 371
MmeI TCCRAC 1 cut(s) 723
Mox20I TGGCCA 1 cut(s) 845
Mph1103I ATGCAT 1 cut(s) 89
MroI TCCGGA 1 cut(s) 605
MroXI GAANNNNTTC 3 cut(s) 128, 702, 1358
MscI TGGCCA 1 cut(s) 845
MseI TTAA 1 cut(s) 207
MslI CAYNNNNRTG 1 cut(s) 1233
Msp20I TGGCCA 1 cut(s) 845
MspA1I CMGCKG 2 cut(s) 659, 1442
MspI CCGG 2 cut(s) 606, 1522
MspR9I CCNGG 1 cut(s) 1311
MunI CAATTG 1 cut(s) 567
MvaI CCWGG 1 cut(s) 1311
NdeI CATATG 1 cut(s) 1161
NdeII GATC 3 cut(s) 184, 286, 1497
NlaIII CATG 7 cut(s) 27, 297, 369, 850, 1244, 1451, 1460
NlaIV GGNNCC 1 cut(s) 263
NmuCI GTSAC 1 cut(s) 1505
NsiI ATGCAT 1 cut(s) 89
NspI RCATGY 1 cut(s) 297
NspV TTCGAA 1 cut(s) 1106
PagI TCATGA 1 cut(s) 1447
PciSI GCTCTTC 1 cut(s) 798
PdmI GAANNNNTTC 3 cut(s) 128, 702, 1358
PfeI GAWTC 5 cut(s) 451, 653, 988, 1061, 1115
PleI GAGTC 2 cut(s) 312, 370
PpsI GAGTC 2 cut(s) 312, 370
Psp6I CCWGG 1 cut(s) 1309
PspGI CCWGG 1 cut(s) 1309
PspN4I GGNNCC 1 cut(s) 263
PstI CTGCAG 2 cut(s) 864, 1386
PstNI CAGNNNCTG 1 cut(s) 662
PvuII CAGCTG 2 cut(s) 659, 1442
RsaI GTAC 3 cut(s) 541, 902, 1189
RsaNI GTAC 3 cut(s) 540, 901, 1188
RseI CAYNNNNRTG 1 cut(s) 1233
SapI GCTCTTC 1 cut(s) 798
SaqAI TTAA 1 cut(s) 207
Sau3AI GATC 3 cut(s) 184, 286, 1497
SchI GAGTC 2 cut(s) 313, 371
ScrFI CCNGG 1 cut(s) 1311
SduI GDGCHC 3 cut(s) 389, 754, 1310
SfaNI GCATC 5 cut(s) 418, 611, 670, 1119, 1405
SfcI CTRYAG 4 cut(s) 573, 860, 1363, 1382
SfuI TTCGAA 1 cut(s) 1106
SmiMI CAYNNNNRTG 1 cut(s) 1233
SmlI CTYRAG 2 cut(s) 301, 597
SmoI CTYRAG 2 cut(s) 301, 597
Sse9I AATT 7 cut(s) 244, 350, 444, 471, 567, 923, 1030
SspI AATATT 1 cut(s) 1177
SspMI CTAG 1 cut(s) 483
StyD4I CCNGG 1 cut(s) 1309
TaaI ACNGT 4 cut(s) 46, 900, 1050, 1187
TaqI TCGA 5 cut(s) 113, 383, 756, 1106, 1500
TasI AATT 7 cut(s) 244, 350, 444, 471, 567, 923, 1030
TfiI GAWTC 5 cut(s) 451, 653, 988, 1061, 1115
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 1 cut(s) 1055
TseFI GTSAC 1 cut(s) 1505
Tsp45I GTSAC 1 cut(s) 1505
TspDTI ATGAA 9 cut(s) 735, 849, 951, 1043, 1059, 1158, 1197, 1436, 1445
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 1 cut(s) 1055
XapI RAATTY 4 cut(s) 244, 471, 923, 1030
XceI RCATGY 1 cut(s) 297
XmnI GAANNNNTTC 3 cut(s) 128, 702, 1358
XspI CTAG 1 cut(s) 483
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.