MD05G1174700.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008250
Physical Location & Seq
Reverse (-)
30272150 .. 30275706
3557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1174700.v1.1.491

Sequence Viewer

Length: 1749 bp
ATGGCTTTTGCTTGCCGGGAAGTTCAGCCATGGACCTTTACTGTTCTTTTGGGAGCTTTTATCGACCTGGGTTTGGCTTATATCCTGCTATGCGTGTCTGCTTTCGTGTTTTTCGCTTCCAAGTTGTTGGGGATTTTCTGGGTTTGTTTTCCATGTCCATGTAATGGGGTTTTCGGGTATAGAAACCCTGATCTTTGCTTGCATAAGTTGTTGCTTATTGATGGGCCTGTTGGGACGATCCGTGCTGTCCATAAGTTGGTGAATAGCAAGTTCCCGTTTGATGTTCTTTGGTCTAGGGATCAAAGGTGCAACTTGAATACGAAACTGATCAGAGATGTAAATTGTGAGAATAGAGTTCGTGAGTTGCAAGGTGAAGCAGATTCTAGTCCATTTTCGAGTCCCAGATTGCAAAACCTGGTTGATAGGGAAAGTGGGTACGACGCTAAGGGGAAGAGGGTTATGGTTCTGAAGAAAAGGACTGGAATTCGGTGCTCTAAGAGGGCGGCTCCTGAATATGGAAGGCTTCCTTCTTACTATCCAAGTGATAGTTCAAAGTTGGTTGCTCACATATTCCCCTTTCCTTGCGATGACAGAGATACAAGAGAGATGTCAGGTGAAAGCTCAATTGCCATAGCTGGTAGAGAAGATTATGTGAAAGCACCAACTGGAAATGATATGGCTGAGAGTATATGCCAAAGTTCTGAATTGAGTGGATCATTTGGTGAGAGTAAAGACGTGGACATTAGTTATACGCAAGAAAAGTCTCACGTTGTTGGAAAGGAAACTGATAAACTCAGGGTGTTGCAAGAAGCACTGGAAAAAGAGAAAGCTGCTTGTGCTGCTCTTTATCTAGAGCTAGAGAAAGAGCGAGCTGCTGCTGCAAGTGCTGCTGATGAAGCAATGTCCATGATAGCACGTCTGCAAAAGGATAAAGCATCAATAGAAATGGAAGTGACGCAGTATCAGAGGATGATGGAAGAAAAATTTGTTTATGATGAAGAGGAGATGGGTGTTCTGAAAGAGATTCTTCTTAGGAGGGAGATGGAGAACCATTTTTTGGAGAGGGAAGTTGAATCGTATAGGCAAATGAGCTTCTTAGGAAATGAATGGTCTGATGCTGATTTGAGTGCTATGTTGTGTGAATCGGGAAAAAGGCCTTCACCTTCTGTTAAGTCAAATACAGATGCACTACTGACGCTGCAGCAGACTGCTGATGGTAAATCTAACTGTATGGAATTCGGAAATATTGCAAATAGTACTTCTCAATATGACGCTTCATTTGTTGAAAAACAAATTCATCCTAATGAACAGGATTCACTTGAGAAATGTGTACTTTCAGAAAGGGAAGAAAAAGTACACACGGATAATGTAATGTGCCAAGAAATAACTCCTGAAGCAGCCCAAGCTCATATTGGTACTGAGGAAAACTTACAATGTGATGGAGTGCGATGGCATGATGGGGATCTTCAGAGAAATTTGCATGGTTCAATGCTTGATATTGAGCCAGCTTTTTATGATGTTCATGTCGTTGATGGTAAAACTGAACTTTGGAAAGAAGAAAGTAGATCATCATTTTATACTGCATTGGATGGATCCCAAGATTTTGCTTCTACATTTGGAGCTACCGGGGTCAGGAGCAGTGAGTTATTACAAGGTTTTCCAAGTACAAGCCAGGTGGATACTGAACCTAAATTCATTGCAAGTTCTTTGGATATGCATTGTGGGTTACCGATGATGGATAACTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

583

Amino Acids

65.27

Weight (kDa)

4.95

Isoelectric Point (pI)

53.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 268 - 357 3.2e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 164, 256, 1057
AciI CCGC 1 cut(s) 503
AclWI GGATC 6 cut(s) 232, 306, 721, 1470, 1587, 1600
AcsI RAATTY 6 cut(s) 483, 983, 1235, 1293, 1474, 1691
AcuI CTGAAG 3 cut(s) 488, 1413, 1451
AfaI GTAC 6 cut(s) 437, 1258, 1332, 1356, 1417, 1666
AfiI CCNNNNNNNGG 6 cut(s) 73, 164, 256, 515, 1057, 1632
AgsI TTSAA 5 cut(s) 316, 552, 1073, 1286, 1488
AjiI CACGTC 2 cut(s) 736, 917
AjnI CCWGG 3 cut(s) 66, 414, 1671
AjuI GAANNNNNNNTTGG 4 cut(s) 532, 564, 1040, 1072
Alw21I GWGCWC 1 cut(s) 494
Alw26I GTCTC 1 cut(s) 768
AlwI GGATC 6 cut(s) 232, 306, 721, 1470, 1587, 1600
AoxI GGCC 2 cut(s) 224, 1154
ApeKI GCWGC 9 cut(s) 830, 839, 872, 875, 878, 887, 1198, 1201, 1397
ApoI RAATTY 6 cut(s) 483, 983, 1235, 1293, 1474, 1691
ArsI GACNNNNNNTTYG 2 cut(s) 1262, 1294
AspS9I GGNCC 2 cut(s) 33, 224
AsuC2I CCSGG 2 cut(s) 17, 1627
AsuHPI GGTGA 5 cut(s) 271, 383, 626, 734, 1152
AvaII GGWCC 1 cut(s) 33
BamHI GGATCC 1 cut(s) 1592
BarI GAAGNNNNNNTAC 2 cut(s) 1338, 1370
Bbv12I GWGCWC 1 cut(s) 494
BbvI GCAGC 9 cut(s) 817, 826, 859, 862, 865, 874, 1185, 1213, 1409
BciT130I CCWGG 3 cut(s) 68, 416, 1673
BciVI GTATCC 1 cut(s) 1672
BclI TGATCA 1 cut(s) 327
BcnI CCSGG 2 cut(s) 17, 1627
BcoDI GTCTC 1 cut(s) 768
BfaI CTAG 4 cut(s) 294, 384, 851, 857
BfmI CTRYAG 1 cut(s) 1199
BfuI GTATCC 1 cut(s) 1672
BmcAI AGTACT 1 cut(s) 1258
Bme1390I CCNGG 5 cut(s) 17, 68, 416, 1627, 1673
Bme18I GGWCC 1 cut(s) 33
BmgBI CACGTC 2 cut(s) 736, 917
BmgT120I GGNCC 2 cut(s) 33, 224
BmiI GGNNCC 2 cut(s) 507, 1594
BmrFI CCNGG 5 cut(s) 17, 68, 416, 1627, 1673
BmsI GCATC 3 cut(s) 944, 1105, 1174
BplI GAGNNNNNCTC 2 cut(s) 490, 522
Bpu10I CCTNAGC 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 1340
BpuMI CCSGG 2 cut(s) 17, 1627
BsaBI GATNNNNATC 1 cut(s) 1461
BsaJI CCNNGG 3 cut(s) 29, 67, 1626
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 164, 256, 515, 1057, 1632
Bse1I ACTGG 3 cut(s) 484, 670, 819
Bse3DI GCAATG 2 cut(s) 906, 1695
Bse8I GATNNNNATC 1 cut(s) 1461
BseBI CCWGG 3 cut(s) 68, 416, 1673
BseDI CCNNGG 3 cut(s) 29, 67, 1626
BseGI GGATG 3 cut(s) 975, 1297, 1594
BseJI GATNNNNATC 1 cut(s) 1461
BseLI CCNNNNNNNGG 6 cut(s) 73, 164, 256, 515, 1057, 1632
BseMI GCAATG 2 cut(s) 906, 1695
BseMII CTCAG 3 cut(s) 672, 808, 1410
BseNI ACTGG 3 cut(s) 484, 670, 819
BseRI GAGGAG 1 cut(s) 1016
BseXI GCAGC 9 cut(s) 817, 826, 859, 862, 865, 874, 1185, 1213, 1409
BshFI GGCC 2 cut(s) 226, 1156
BsiHKAI GWGCWC 1 cut(s) 494
BsiSI CCGG 2 cut(s) 16, 1626
BslFI GGGAC 2 cut(s) 247, 384
BslI CCNNNNNNNGG 6 cut(s) 73, 164, 256, 515, 1057, 1632
BsmAI GTCTC 1 cut(s) 768
BsmFI GGGAC 2 cut(s) 247, 384
BsnI GGCC 2 cut(s) 226, 1156
Bsp1286I GDGCHC 1 cut(s) 494
Bsp143I GATC 8 cut(s) 190, 237, 298, 327, 713, 1462, 1565, 1592
Bsp19I CCATGG 1 cut(s) 29
BspACI CCGC 1 cut(s) 503
BspANI GGCC 2 cut(s) 226, 1156
BspCNI CTCAG 3 cut(s) 673, 807, 1411
BspLI GGNNCC 2 cut(s) 507, 1594
BspMAI CTGCAG 1 cut(s) 1203
BspPI GGATC 6 cut(s) 232, 306, 721, 1470, 1587, 1600
BsrDI GCAATG 2 cut(s) 906, 1695
BsrI ACTGG 3 cut(s) 484, 670, 819
BssECI CCNNGG 3 cut(s) 29, 67, 1626
BssMI GATC 8 cut(s) 190, 237, 298, 327, 713, 1462, 1565, 1592
BssT1I CCWWGG 1 cut(s) 29
Bst2UI CCWGG 3 cut(s) 68, 416, 1673
Bst4CI ACNGT 2 cut(s) 43, 1229
Bst6I CTCTTC 2 cut(s) 446, 993
BstAPI GCANNNNNTGC 1 cut(s) 887
BstC8I GCNNGC 4 cut(s) 13, 200, 870, 1506
BstDEI CTNAG 7 cut(s) 444, 495, 681, 794, 1031, 1096, 1419
BstDSI CCRYGG 1 cut(s) 29
BstEII GGTNACC 1 cut(s) 1725
BstF5I GGATG 3 cut(s) 975, 1297, 1594
BstKTI GATC 8 cut(s) 193, 240, 301, 330, 716, 1465, 1568, 1595
BstMAI GTCTC 1 cut(s) 768
BstMBI GATC 8 cut(s) 190, 237, 298, 327, 713, 1462, 1565, 1592
BstMWI GCNNNNNNNGC 7 cut(s) 836, 839, 878, 884, 887, 896, 1403
BstNI CCWGG 3 cut(s) 68, 416, 1673
BstPI GGTNACC 1 cut(s) 1725
BstSCI CCNGG 5 cut(s) 15, 66, 414, 1625, 1671
BstSFI CTRYAG 1 cut(s) 1199
BstV1I GCAGC 9 cut(s) 817, 826, 859, 862, 865, 874, 1185, 1213, 1409
BstX2I RGATCY 2 cut(s) 1462, 1592
BstXI CCANNNNNNTGG 1 cut(s) 127
BstYI RGATCY 2 cut(s) 1462, 1592
BsuI GTATCC 1 cut(s) 1672
BsuRI GGCC 2 cut(s) 226, 1156
BtgI CCRYGG 1 cut(s) 29
BtgZI GCGATG 2 cut(s) 600, 1462
BtrI CACGTC 2 cut(s) 736, 917
BtsCI GGATG 3 cut(s) 975, 1297, 1594
BtsI GCAGTG 1 cut(s) 1645
BtsIMutI CAGTG 2 cut(s) 812, 1645
Cac8I GCNNGC 4 cut(s) 13, 200, 870, 1506
Cfr13I GGNCC 2 cut(s) 33, 224
CseI GACGC 4 cut(s) 449, 964, 1204, 1280
CsiI ACCWGGT 1 cut(s) 414
Csp6I GTAC 6 cut(s) 436, 1257, 1331, 1355, 1416, 1665
CspCI CAANNNNNGTGG 2 cut(s) 1656, 1691
CviAII CATG 7 cut(s) 30, 153, 159, 907, 1454, 1481, 1523
CviQI GTAC 6 cut(s) 436, 1257, 1331, 1355, 1416, 1665
DdeI CTNAG 7 cut(s) 444, 495, 681, 794, 1031, 1096, 1419
DpnI GATC 8 cut(s) 192, 239, 300, 329, 715, 1464, 1567, 1594
DpnII GATC 8 cut(s) 190, 237, 298, 327, 713, 1462, 1565, 1592
Eam1104I CTCTTC 2 cut(s) 446, 993
EarI CTCTTC 2 cut(s) 446, 993
Eco130I CCWWGG 1 cut(s) 29
Eco147I AGGCCT 1 cut(s) 1156
Eco47I GGWCC 1 cut(s) 33
Eco57I CTGAAG 3 cut(s) 488, 1413, 1451
Eco91I GGTNACC 1 cut(s) 1725
EcoO65I GGTNACC 1 cut(s) 1725
EcoRI GAATTC 2 cut(s) 483, 1235
EcoRII CCWGG 3 cut(s) 66, 414, 1671
EcoT14I CCWWGG 1 cut(s) 29
EcoT22I ATGCAT 1 cut(s) 1719
ErhI CCWWGG 1 cut(s) 29
FaeI CATG 7 cut(s) 33, 156, 162, 910, 1457, 1484, 1526
FalI AAGNNNNNCTT 4 cut(s) 511, 543, 1011, 1043
FaqI GGGAC 2 cut(s) 247, 384
FatI CATG 7 cut(s) 29, 152, 158, 906, 1453, 1480, 1522
FbaI TGATCA 1 cut(s) 327
FokI GGATG 3 cut(s) 982, 1284, 1601
FspBI CTAG 4 cut(s) 294, 384, 851, 857
HaeIII GGCC 2 cut(s) 226, 1156
HapII CCGG 2 cut(s) 16, 1626
HgaI GACGC 4 cut(s) 449, 964, 1204, 1280
Hin1II CATG 7 cut(s) 33, 156, 162, 910, 1457, 1484, 1526
HinfI GANTC 6 cut(s) 380, 397, 1024, 1073, 1142, 1313
HpaII CCGG 2 cut(s) 16, 1626
HphI GGTGA 5 cut(s) 271, 383, 626, 734, 1152
Hpy166II GTNNAC 3 cut(s) 739, 1331, 1357
Hpy188I TCNGA 9 cut(s) 332, 468, 703, 966, 1017, 1114, 1241, 1339, 1470
Hpy188III TCNNGA 7 cut(s) 359, 509, 851, 1146, 1391, 1633, 1746
Hpy8I GTNNAC 3 cut(s) 739, 1331, 1357
Hpy99I CGWCG 1 cut(s) 443
HpyAV CCTTC 4 cut(s) 513, 537, 1167, 1173
HpyCH4III ACNGT 2 cut(s) 43, 1229
HpyCH4IV ACGT 3 cut(s) 735, 768, 916
HpyF10VI GCNNNNNNNGC 7 cut(s) 836, 839, 878, 884, 887, 896, 1403
HpyF3I CTNAG 7 cut(s) 444, 495, 681, 794, 1031, 1096, 1419
HpySE526I ACGT 3 cut(s) 735, 768, 916
Hsp92II CATG 7 cut(s) 33, 156, 162, 910, 1457, 1484, 1526
Ksp22I TGATCA 1 cut(s) 327
Kzo9I GATC 8 cut(s) 190, 237, 298, 327, 713, 1462, 1565, 1592
LmnI GCTCC 4 cut(s) 53, 511, 1619, 1635
Lsp1109I GCAGC 9 cut(s) 817, 826, 859, 862, 865, 874, 1185, 1213, 1409
LweI GCATC 3 cut(s) 944, 1105, 1174
MabI ACCWGGT 1 cut(s) 414
MaeI CTAG 4 cut(s) 294, 384, 851, 857
MaeII ACGT 3 cut(s) 735, 768, 916
MaeIII GTNAC 2 cut(s) 952, 1725
MalI GATC 8 cut(s) 192, 239, 300, 329, 715, 1464, 1567, 1594
MboI GATC 8 cut(s) 190, 237, 298, 327, 713, 1462, 1565, 1592
MboII GAAGA 9 cut(s) 463, 481, 656, 989, 1010, 1019, 1358, 1457, 1568
MfeI CAATTG 1 cut(s) 624
MflI RGATCY 2 cut(s) 1462, 1592
MhlI GDGCHC 1 cut(s) 494
MluCI AATT 9 cut(s) 340, 483, 624, 704, 983, 1235, 1293, 1474, 1691
MlyI GAGTC 1 cut(s) 406
MmeI TCCRAC 1 cut(s) 754
MnlI CCTC 7 cut(s) 447, 492, 960, 994, 1029, 1056, 1414
Mph1103I ATGCAT 1 cut(s) 1719
MseI TTAA 1 cut(s) 1170
MslI CAYNNNNRTG 2 cut(s) 157, 1302
MspI CCGG 2 cut(s) 16, 1626
MspR9I CCNGG 5 cut(s) 17, 68, 416, 1627, 1673
MunI CAATTG 1 cut(s) 624
MvaI CCWGG 3 cut(s) 68, 416, 1673
MwoI GCNNNNNNNGC 7 cut(s) 836, 839, 878, 884, 887, 896, 1403
NciI CCSGG 2 cut(s) 17, 1627
NcoI CCATGG 1 cut(s) 29
NdeII GATC 8 cut(s) 190, 237, 298, 327, 713, 1462, 1565, 1592
NlaIII CATG 7 cut(s) 33, 156, 162, 910, 1457, 1484, 1526
NlaIV GGNNCC 2 cut(s) 507, 1594
NmuCI GTSAC 1 cut(s) 952
NsiI ATGCAT 1 cut(s) 1719
PceI AGGCCT 1 cut(s) 1156
PfeI GAWTC 5 cut(s) 380, 1024, 1073, 1142, 1313
PflMI CCANNNNNTGG 3 cut(s) 164, 256, 1057
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
Psp6I CCWGG 3 cut(s) 66, 414, 1671
PspEI GGTNACC 1 cut(s) 1725
PspGI CCWGG 3 cut(s) 66, 414, 1671
PspN4I GGNNCC 2 cut(s) 507, 1594
PspPI GGNCC 2 cut(s) 33, 224
PstI CTGCAG 1 cut(s) 1203
PsuI RGATCY 2 cut(s) 1462, 1592
RsaI GTAC 6 cut(s) 437, 1258, 1332, 1356, 1417, 1666
RsaNI GTAC 6 cut(s) 436, 1257, 1331, 1355, 1416, 1665
RseI CAYNNNNRTG 2 cut(s) 157, 1302
SaqAI TTAA 1 cut(s) 1170
Sau3AI GATC 8 cut(s) 190, 237, 298, 327, 713, 1462, 1565, 1592
Sau96I GGNCC 2 cut(s) 33, 224
ScaI AGTACT 1 cut(s) 1258
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 5 cut(s) 17, 68, 416, 1627, 1673
SduI GDGCHC 1 cut(s) 494
SexAI ACCWGGT 1 cut(s) 414
SfaNI GCATC 3 cut(s) 944, 1105, 1174
SfcI CTRYAG 1 cut(s) 1199
SinI GGWCC 1 cut(s) 33
SmiMI CAYNNNNRTG 2 cut(s) 157, 1302
SmlI CTYRAG 1 cut(s) 1319
SmoI CTYRAG 1 cut(s) 1319
Sse9I AATT 9 cut(s) 340, 483, 624, 704, 983, 1235, 1293, 1474, 1691
SseBI AGGCCT 1 cut(s) 1156
SsiI CCGC 1 cut(s) 503
SspI AATATT 1 cut(s) 1246
SspMI CTAG 4 cut(s) 294, 384, 851, 857
StuI AGGCCT 1 cut(s) 1156
StyD4I CCNGG 5 cut(s) 15, 66, 414, 1625, 1671
StyI CCWWGG 1 cut(s) 29
TaaI ACNGT 2 cut(s) 43, 1229
TaiI ACGT 3 cut(s) 738, 771, 919
TaqI TCGA 2 cut(s) 63, 395
TasI AATT 9 cut(s) 340, 483, 624, 704, 983, 1235, 1293, 1474, 1691
TatI WGTACW 4 cut(s) 1256, 1330, 1354, 1664
TauI GCSGC 1 cut(s) 506
TfiI GAWTC 5 cut(s) 380, 1024, 1073, 1142, 1313
Tru1I TTAA 1 cut(s) 1170
Tru9I TTAA 1 cut(s) 1170
TscAI CASTG 2 cut(s) 819, 1645
TseFI GTSAC 1 cut(s) 952
TseI GCWGC 9 cut(s) 830, 839, 872, 875, 878, 887, 1198, 1201, 1397
Tsp45I GTSAC 1 cut(s) 952
TspDTI ATGAA 8 cut(s) 909, 1011, 1119, 1266, 1286, 1320, 1511, 1684
TspGWI ACGGA 2 cut(s) 230, 1376
TspRI CASTG 2 cut(s) 819, 1645
Van91I CCANNNNNTGG 3 cut(s) 164, 256, 1057
VpaK11BI GGWCC 1 cut(s) 33
XapI RAATTY 6 cut(s) 483, 983, 1235, 1293, 1474, 1691
XbaI TCTAGA 1 cut(s) 850
XcmI CCANNNNNNNNNTGG 1 cut(s) 1409
XspI CTAG 4 cut(s) 294, 384, 851, 857
ZrmI AGTACT 1 cut(s) 1258
Zsp2I ATGCAT 1 cut(s) 1719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.