MD10G1163900.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008250
Physical Location & Seq
Reverse (-)
25477678 .. 25481421
3744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1163900.v1.1.491

Sequence Viewer

Length: 1968 bp
ATGGCTTTTGCTTGCCAAGAAGTTCAGCCCTGGACCTTTGCTGGTCTTATACGAGCTTTTCTCGACCTGGGTTTGGCTTATTTCCTGCTGTGTGTGTCTGCTTTCATGTTTTTCGCCTCGAAGTTGTTGAGGATTTTTTGGGTTTATTTGCCATGTCCTTGTAATGGGGTTTTCGGGTATCGAAACCGTGATCTTTGCTTACATAAGTTGCTTATTGACCGGCCTGTTGGGACGATCGGCGATGTTCACAAGCTGGTGAAGAGCAGGTTCCCTTTTGATGTTATTTGGTCTCGGGATCAAGGATGCAACTTGAATAGGAAACTGATCAGAGATGTGAATTGTGAGAATGGAGTCCATGAGTTGGAAGGTGAAGCGGATTCTAGTCCGTTTTCGAGTCCCAGATTGCAAAACCTGGTTGATAGGGAAAGTGGGTATGATGCTAAGGGGAAGAGGGTAATGACTCTAAAGAAAAGGACTGGAATTCGGCGCTCTAGGAGAGCCGCTCCTGAATACGGAAGGCTTTCTTCTTACTATCCAAGTGATAGTTCAAGGTTGGTTGCTCGCATATTCCCCTTTCCTTGCGATAACAGAGATACAAGAGAGATGTCGGGTGAAAGCTCAGTTGCCATAGCTGGTAGAGAAGATTATGTGAAAGCATCAGCTGGAGATGATATGGCTGAAAGTATATGCCAAAGTTCTGAATTGAGTGGATTATTTGGTGCGAGTAAGGACATGGATGTTAGTAACACGCAAGAAAAGGCTTCCGTTGTTGGAAATGAAACTGATAAACTCAGGGTGTTGGAAGAAGCACTGGAAAAAGAGAAAGCTGCTTGTGCTACTCTTTATCTAGAGCTAGAGAAAGAGCGAGCTGCTGCTGCAAGTGCTGCTGATGAAGCAATGGCCATGATAGCCCGTCTGCAAAAGGATAAAGCATCAATAGAAATGGAAGTGAGGCAGTACCAAAGGATGATCGAAGAAAAATTTATTTATGATGAAGAGGAGATGGATGTTCTGAAAGAGATTCTTCTTAGGAGGGAGATGGAGAACCATTTTTTGGAGAAGGAAGTTGAATCGTATAGGCAGATGGGTGTCTTAGGAAAGGAACGGTCTGATGCTGATTTGAGTGAAATGTTATGTGAATCGGGAAAAAGGCCTTCACCTTCACTTGATCCAAATACAGATGCACAACTGATGCTAAAGCAGACTGCTGATAGTAAATCCAACTGTACGGCAATCGAAAATATTGCAAATAGTACTTCTCAGTATGAGGCTTCATTTGTTGAAAAACAAATCCATCCTAATGAACATGATTCACTTGAGAAATGTATACTTTCAGAAAGGGGAGAAAAAGTACACACGGATAATGTAATGTGCCAAGAAATGACTACTGAAGCAGCCCAAGCTCATAATGGTACCGAGGAAAACTTACATTGTGATGAAGAAGTGCCACAGCTTGATGGGGATCTTCAGAGGAATTTGCATGGTTCAATGCTTGATATTGAGCCAGCTGTTTATGATGTTCATGTCGTTGATGGTAAAACTGAACTTTGGAAAGAAGAAAGTAGATCATCACTTTATACTGCATTGGATGGATCCCAAGATTTCACTCATACATTTGGCGCTGCCGGAGTCAGTAGCAGTGAGTTATTACAAGGTTTGCCAAGTACAAGCCAGGTGGATACTGAACCATTCATTCCAAGTTCTTTGGATATGCATTGTGGGTTATCGATGTTGGATAGCTCCCGATGCAAAACCTTGGTGATAGACTCAATGAAGAACTCATTGTCGACAGTAAATAGTGAAAGGTCGAAGATTGACACTGAGGTTGAATTGCTAATGGAAAAGCTACAGATTCTAGAAGAGAGAAAAGAGAAGTTGACTTCCTCTTCTGAACATGGGGAGAGGCAAAAAACCCCATCAAAATGTTTGGAGGAGATCAGAACCCGCTCTGCAAGATTCAGCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

656

Amino Acids

73.72

Weight (kDa)

5.08

Isoelectric Point (pI)

54.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 267 - 356 4.4e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 255
Acc65I GGTACC 1 cut(s) 1412
AccB1I GGYRCC 1 cut(s) 1412
AccB7I CCANNNNNTGG 2 cut(s) 361, 1054
AccBSI CCGCTC 2 cut(s) 503, 1947
AccI GTMKAC 2 cut(s) 1327, 1787
AciI CCGC 3 cut(s) 374, 501, 1945
AclWI GGATC 5 cut(s) 303, 1163, 1470, 1587, 1600
AcoI YGGCCR 1 cut(s) 900
AcsI RAATTY 3 cut(s) 480, 980, 1474
AcuI CTGAAG 2 cut(s) 1410, 1451
AfaI GTAC 6 cut(s) 959, 1228, 1255, 1353, 1414, 1666
AfiI CCNNNNNNNGG 5 cut(s) 73, 164, 361, 512, 1054
AgsI TTSAA 6 cut(s) 313, 549, 1070, 1283, 1488, 1829
AjnI CCWGG 4 cut(s) 29, 66, 411, 1671
AjuI GAANNNNNNNTTGG 4 cut(s) 529, 561, 1037, 1069
Alw26I GTCTC 1 cut(s) 294
AlwI GGATC 5 cut(s) 303, 1163, 1470, 1587, 1600
Ama87I CYCGRG 1 cut(s) 291
AoxI GGCC 3 cut(s) 221, 900, 1151
ApeKI GCWGC 7 cut(s) 827, 869, 872, 875, 884, 1394, 1622
ApoI RAATTY 3 cut(s) 480, 980, 1474
Asp700I GAANNNNTTC 1 cut(s) 520
Asp718I GGTACC 1 cut(s) 1412
AspLEI GCGC 2 cut(s) 489, 1622
AspS9I GGNCC 1 cut(s) 33
AsuHPI GGTGA 5 cut(s) 268, 380, 623, 1149, 1771
AvaI CYCGRG 1 cut(s) 291
AvaII GGWCC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 902
BamHI GGATCC 1 cut(s) 1592
BanI GGYRCC 1 cut(s) 1412
BbvI GCAGC 7 cut(s) 814, 856, 859, 862, 871, 1406, 1609
BccI CCATC 8 cut(s) 997, 1033, 1078, 1302, 1451, 1526, 1583, 1924
BceAI ACGGC 1 cut(s) 1245
BcgI CGANNNNNNTGC 2 cut(s) 1226, 1260
BciT130I CCWGG 4 cut(s) 31, 68, 413, 1673
BciVI GTATCC 1 cut(s) 1672
BclI TGATCA 1 cut(s) 324
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 5 cut(s) 381, 492, 848, 854, 1856
BfmI CTRYAG 1 cut(s) 1847
BfoI RGCGCY 2 cut(s) 490, 1623
BfuAI ACCTGC 1 cut(s) 255
BfuI GTATCC 1 cut(s) 1672
BisI GCNGC 8 cut(s) 501, 828, 870, 873, 876, 885, 1395, 1623
BlsI GCNGC 8 cut(s) 502, 829, 871, 874, 877, 886, 1396, 1624
BmcAI AGTACT 1 cut(s) 1255
Bme1390I CCNGG 4 cut(s) 31, 68, 413, 1673
Bme18I GGWCC 1 cut(s) 33
BmeT110I CYCGRG 1 cut(s) 291
BmgT120I GGNCC 1 cut(s) 33
BmiI GGNNCC 3 cut(s) 269, 1414, 1594
BmrFI CCNGG 4 cut(s) 31, 68, 413, 1673
BmsI GCATC 8 cut(s) 293, 427, 665, 941, 1102, 1171, 1182, 1736
BplI GAGNNNNNCTC 4 cut(s) 45, 77, 487, 519
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 1337
Bsa29I ATCGAT 1 cut(s) 1727
BsaBI GATNNNNATC 1 cut(s) 1461
BsaI GGTCTC 1 cut(s) 294
BsaJI CCNNGG 4 cut(s) 29, 67, 1416, 1755
BsaXI ACNNNNNCTCC 2 cut(s) 1335, 1365
Bsc4I CCNNNNNNNGG 5 cut(s) 73, 164, 361, 512, 1054
Bse118I RCCGGY 1 cut(s) 219
Bse1I ACTGG 2 cut(s) 481, 816
Bse3DI GCAATG 1 cut(s) 903
Bse8I GATNNNNATC 1 cut(s) 1461
BseBI CCWGG 4 cut(s) 31, 68, 413, 1673
BseCI ATCGAT 1 cut(s) 1727
BseDI CCNNGG 4 cut(s) 29, 67, 1416, 1755
BseGI GGATG 6 cut(s) 308, 742, 972, 1012, 1294, 1594
BseJI GATNNNNATC 1 cut(s) 1461
BseLI CCNNNNNNNGG 5 cut(s) 73, 164, 361, 512, 1054
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 4 cut(s) 633, 805, 1274, 1812
BseNI ACTGG 2 cut(s) 481, 816
BseRI GAGGAG 2 cut(s) 1013, 1946
BseXI GCAGC 7 cut(s) 814, 856, 859, 862, 871, 1406, 1609
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 3 cut(s) 223, 902, 1153
BshNI GGYRCC 1 cut(s) 1412
BshVI ATCGAT 1 cut(s) 1727
BsiEI CGRYCG 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 291
BsiSI CCGG 2 cut(s) 220, 1626
BslFI GGGAC 2 cut(s) 244, 381
BslI CCNNNNNNNGG 5 cut(s) 73, 164, 361, 512, 1054
BsmAI GTCTC 1 cut(s) 294
BsmFI GGGAC 2 cut(s) 244, 381
BsnI GGCC 3 cut(s) 223, 902, 1153
Bso31I GGTCTC 1 cut(s) 294
BsoBI CYCGRG 1 cut(s) 291
BspACI CCGC 3 cut(s) 374, 501, 1945
BspANI GGCC 3 cut(s) 223, 902, 1153
BspCNI CTCAG 4 cut(s) 632, 804, 1273, 1813
BspDI ATCGAT 1 cut(s) 1727
BspLI GGNNCC 3 cut(s) 269, 1414, 1594
BspMI ACCTGC 1 cut(s) 255
BspPI GGATC 5 cut(s) 303, 1163, 1470, 1587, 1600
BspQI GCTCTTC 1 cut(s) 254
BspT107I GGYRCC 1 cut(s) 1412
BspTNI GGTCTC 1 cut(s) 294
BsrBI CCGCTC 2 cut(s) 503, 1947
BsrDI GCAATG 1 cut(s) 903
BsrFI RCCGGY 1 cut(s) 219
BsrI ACTGG 2 cut(s) 481, 816
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 4 cut(s) 29, 67, 1416, 1755
BssNAI GTATAC 1 cut(s) 1328
BssT1I CCWWGG 1 cut(s) 1755
Bst1107I GTATAC 1 cut(s) 1328
Bst2UI CCWGG 4 cut(s) 31, 68, 413, 1673
Bst4CI ACNGT 4 cut(s) 188, 1107, 1226, 1792
Bst6I CTCTTC 5 cut(s) 254, 443, 990, 1854, 1891
BstAPI GCANNNNNTGC 1 cut(s) 884
BstC8I GCNNGC 4 cut(s) 13, 562, 867, 1506
BstDEI CTNAG 7 cut(s) 441, 619, 791, 1028, 1093, 1260, 1821
BstF5I GGATG 6 cut(s) 308, 742, 972, 1012, 1294, 1594
BstH2I RGCGCY 2 cut(s) 490, 1623
BstHHI GCGC 2 cut(s) 489, 1622
BstMAI GTCTC 1 cut(s) 294
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 8 cut(s) 833, 875, 881, 884, 893, 908, 1400, 1746
BstNI CCWGG 4 cut(s) 31, 68, 413, 1673
BstSCI CCNGG 4 cut(s) 29, 66, 411, 1671
BstSFI CTRYAG 1 cut(s) 1847
BstV1I GCAGC 7 cut(s) 814, 856, 859, 862, 871, 1406, 1609
BstX2I RGATCY 2 cut(s) 1462, 1592
BstYI RGATCY 2 cut(s) 1462, 1592
BstZ17I GTATAC 1 cut(s) 1328
Bsu15I ATCGAT 1 cut(s) 1727
BsuI GTATCC 1 cut(s) 1672
BsuRI GGCC 3 cut(s) 223, 902, 1153
BsuTUI ATCGAT 1 cut(s) 1727
BtgZI GCGATG 1 cut(s) 255
BtsCI GGATG 6 cut(s) 308, 742, 972, 1012, 1294, 1594
BtsI GCAGTG 1 cut(s) 1645
BtsIMutI CAGTG 3 cut(s) 809, 1645, 1818
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 4 cut(s) 13, 562, 867, 1506
CfoI GCGC 2 cut(s) 489, 1622
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 1 cut(s) 33
ClaI ATCGAT 1 cut(s) 1727
CsiI ACCWGGT 1 cut(s) 411
Csp6I GTAC 6 cut(s) 958, 1227, 1254, 1352, 1413, 1665
CspCI CAANNNNNGTGG 2 cut(s) 1656, 1691
CviAII CATG 9 cut(s) 106, 153, 356, 733, 904, 1307, 1481, 1523, 1895
CviQI GTAC 6 cut(s) 958, 1227, 1254, 1352, 1413, 1665
DdeI CTNAG 7 cut(s) 441, 619, 791, 1028, 1093, 1260, 1821
EaeI YGGCCR 1 cut(s) 900
Eam1104I CTCTTC 5 cut(s) 254, 443, 990, 1854, 1891
EarI CTCTTC 5 cut(s) 254, 443, 990, 1854, 1891
Eco130I CCWWGG 1 cut(s) 1755
Eco147I AGGCCT 1 cut(s) 1153
Eco31I GGTCTC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 33
Eco57I CTGAAG 2 cut(s) 1410, 1451
Eco88I CYCGRG 1 cut(s) 291
EcoRI GAATTC 1 cut(s) 480
EcoRII CCWGG 4 cut(s) 29, 66, 411, 1671
EcoT14I CCWWGG 1 cut(s) 1755
EcoT22I ATGCAT 1 cut(s) 1716
ErhI CCWWGG 1 cut(s) 1755
FaeI CATG 9 cut(s) 109, 156, 359, 736, 907, 1310, 1484, 1526, 1898
FalI AAGNNNNNCTT 4 cut(s) 508, 540, 1008, 1040
FaqI GGGAC 2 cut(s) 244, 381
FatI CATG 9 cut(s) 105, 152, 355, 732, 903, 1306, 1480, 1522, 1894
FauI CCCGC 1 cut(s) 1952
FbaI TGATCA 1 cut(s) 324
FblI GTMKAC 2 cut(s) 1327, 1787
Fnu4HI GCNGC 8 cut(s) 501, 828, 870, 873, 876, 885, 1395, 1623
FokI GGATG 6 cut(s) 315, 749, 979, 1019, 1281, 1601
Fsp4HI GCNGC 8 cut(s) 501, 828, 870, 873, 876, 885, 1395, 1623
FspBI CTAG 5 cut(s) 381, 492, 848, 854, 1856
GlaI GCGC 2 cut(s) 488, 1621
GluI GCNGC 8 cut(s) 501, 828, 870, 873, 876, 885, 1395, 1623
GsuI CTGGAG 1 cut(s) 684
HaeII RGCGCY 2 cut(s) 490, 1623
HaeIII GGCC 3 cut(s) 223, 902, 1153
HapII CCGG 2 cut(s) 220, 1626
HhaI GCGC 2 cut(s) 489, 1622
Hin1II CATG 9 cut(s) 109, 156, 359, 736, 907, 1310, 1484, 1526, 1898
Hin6I GCGC 2 cut(s) 487, 1620
HinP1I GCGC 2 cut(s) 487, 1620
HincII GTYRAC 2 cut(s) 1788, 1878
HindII GTYRAC 2 cut(s) 1788, 1878
HpaII CCGG 2 cut(s) 220, 1626
HphI GGTGA 5 cut(s) 268, 380, 623, 1149, 1771
Hpy166II GTNNAC 5 cut(s) 247, 1328, 1354, 1788, 1878
Hpy188I TCNGA 8 cut(s) 329, 700, 1014, 1111, 1336, 1470, 1891, 1940
Hpy188III TCNNGA 7 cut(s) 62, 293, 506, 848, 1143, 1743, 1856
Hpy8I GTNNAC 5 cut(s) 247, 1328, 1354, 1788, 1878
HpyAV CCTTC 5 cut(s) 359, 510, 1054, 1164, 1170
HpyCH4III ACNGT 4 cut(s) 188, 1107, 1226, 1792
HpyF10VI GCNNNNNNNGC 8 cut(s) 833, 875, 881, 884, 893, 908, 1400, 1746
HpyF3I CTNAG 7 cut(s) 441, 619, 791, 1028, 1093, 1260, 1821
Hsp92II CATG 9 cut(s) 109, 156, 359, 736, 907, 1310, 1484, 1526, 1898
HspAI GCGC 2 cut(s) 487, 1620
KpnI GGTACC 1 cut(s) 1416
Ksp22I TGATCA 1 cut(s) 324
LguI GCTCTTC 1 cut(s) 254
LmnI GCTCC 2 cut(s) 508, 1745
Lsp1109I GCAGC 7 cut(s) 814, 856, 859, 862, 871, 1406, 1609
LweI GCATC 8 cut(s) 293, 427, 665, 941, 1102, 1171, 1182, 1736
MabI ACCWGGT 1 cut(s) 411
MaeI CTAG 5 cut(s) 381, 492, 848, 854, 1856
MaeIII GTNAC 1 cut(s) 743
MbiI CCGCTC 2 cut(s) 503, 1947
MflI RGATCY 2 cut(s) 1462, 1592
MlsI TGGCCA 1 cut(s) 902
MluCI AATT 6 cut(s) 337, 480, 701, 980, 1474, 1829
MluNI TGGCCA 1 cut(s) 902
MlyI GAGTC 5 cut(s) 360, 403, 454, 1638, 1760
MmeI TCCRAC 5 cut(s) 342, 751, 780, 1245, 1713
Mox20I TGGCCA 1 cut(s) 902
Mph1103I ATGCAT 1 cut(s) 1716
MroXI GAANNNNTTC 1 cut(s) 520
MscI TGGCCA 1 cut(s) 902
MslI CAYNNNNRTG 3 cut(s) 1299, 1434, 1921
Msp20I TGGCCA 1 cut(s) 902
MspA1I CMGCKG 2 cut(s) 662, 1508
MspI CCGG 2 cut(s) 220, 1626
MspR9I CCNGG 4 cut(s) 31, 68, 413, 1673
MvaI CCWGG 4 cut(s) 31, 68, 413, 1673
MwoI GCNNNNNNNGC 8 cut(s) 833, 875, 881, 884, 893, 908, 1400, 1746
NlaIII CATG 9 cut(s) 109, 156, 359, 736, 907, 1310, 1484, 1526, 1898
NlaIV GGNNCC 3 cut(s) 269, 1414, 1594
NsiI ATGCAT 1 cut(s) 1716
PceI AGGCCT 1 cut(s) 1153
PciSI GCTCTTC 1 cut(s) 254
PdmI GAANNNNTTC 1 cut(s) 520
PfeI GAWTC 7 cut(s) 377, 1021, 1070, 1139, 1310, 1852, 1956
PflMI CCANNNNNTGG 2 cut(s) 361, 1054
PkrI GCNGC 8 cut(s) 502, 829, 871, 874, 877, 886, 1396, 1624
Ple19I CGATCG 1 cut(s) 237
PleI GAGTC 5 cut(s) 359, 402, 454, 1637, 1760
PpsI GAGTC 5 cut(s) 359, 402, 454, 1637, 1760
Psp6I CCWGG 4 cut(s) 29, 66, 411, 1671
PspGI CCWGG 4 cut(s) 29, 66, 411, 1671
PspN4I GGNNCC 3 cut(s) 269, 1414, 1594
PspPI GGNCC 1 cut(s) 33
PsuI RGATCY 2 cut(s) 1462, 1592
PvuI CGATCG 1 cut(s) 237
PvuII CAGCTG 2 cut(s) 662, 1508
RsaI GTAC 6 cut(s) 959, 1228, 1255, 1353, 1414, 1666
RsaNI GTAC 6 cut(s) 958, 1227, 1254, 1352, 1413, 1665
RseI CAYNNNNRTG 3 cut(s) 1299, 1434, 1921
SalI GTCGAC 1 cut(s) 1786
SapI GCTCTTC 1 cut(s) 254
SatI GCNGC 8 cut(s) 501, 828, 870, 873, 876, 885, 1395, 1623
Sau96I GGNCC 1 cut(s) 33
ScaI AGTACT 1 cut(s) 1255
SchI GAGTC 5 cut(s) 360, 403, 454, 1638, 1760
ScrFI CCNGG 4 cut(s) 31, 68, 413, 1673
SexAI ACCWGGT 1 cut(s) 411
SfaNI GCATC 8 cut(s) 293, 427, 665, 941, 1102, 1171, 1182, 1736
SfcI CTRYAG 1 cut(s) 1847
SinI GGWCC 1 cut(s) 33
SmiMI CAYNNNNRTG 3 cut(s) 1299, 1434, 1921
SmlI CTYRAG 1 cut(s) 1316
SmoI CTYRAG 1 cut(s) 1316
Sse9I AATT 6 cut(s) 337, 480, 701, 980, 1474, 1829
SseBI AGGCCT 1 cut(s) 1153
SsiI CCGC 3 cut(s) 374, 501, 1945
SspI AATATT 1 cut(s) 1243
SspMI CTAG 5 cut(s) 381, 492, 848, 854, 1856
StuI AGGCCT 1 cut(s) 1153
StyD4I CCNGG 4 cut(s) 29, 66, 411, 1671
StyI CCWWGG 1 cut(s) 1755
TaaI ACNGT 4 cut(s) 188, 1107, 1226, 1792
TaqI TCGA 9 cut(s) 63, 119, 181, 392, 972, 1236, 1727, 1787, 1808
TasI AATT 6 cut(s) 337, 480, 701, 980, 1474, 1829
TatI WGTACW 3 cut(s) 1253, 1351, 1664
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 7 cut(s) 377, 1021, 1070, 1139, 1310, 1852, 1956
TscAI CASTG 3 cut(s) 816, 1645, 1825
TseI GCWGC 7 cut(s) 827, 869, 872, 875, 884, 1394, 1622
TspGWI ACGGA 4 cut(s) 375, 528, 754, 1373
TspRI CASTG 3 cut(s) 816, 1645, 1825
Van91I CCANNNNNTGG 2 cut(s) 361, 1054
VpaK11BI GGWCC 1 cut(s) 33
XapI RAATTY 3 cut(s) 480, 980, 1474
XbaI TCTAGA 2 cut(s) 847, 1855
XcmI CCANNNNNNNNNTGG 1 cut(s) 1406
XmiI GTMKAC 2 cut(s) 1327, 1787
XmnI GAANNNNTTC 1 cut(s) 520
XspI CTAG 5 cut(s) 381, 492, 848, 854, 1856
ZrmI AGTACT 1 cut(s) 1255
Zsp2I ATGCAT 1 cut(s) 1716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.