pycom05g16160

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008250
Physical Location & Seq
Reverse (-)
19505541 .. 19506457
917 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g16160.1

Sequence Viewer

Length: 666 bp
ATGGTTCTGAAGAAAAGGACTGGAATTCGGCGCTCTAGGAGGGCGGCTCCTGAATATGAAAGGCTTCCTTCTTACTATCCAAGTGATAGTTCAAAGTTGTTTGCTCACATATTCCCTTTTCCTTGCGATGACAGAGATACAAGAGAGATGTCAGGTGAAAGCTCAATTGCCATAGCTGGTAGAGAAGATTATGTGAAAGCACCAACTGGAAATGATATGGCTGAGTGTATATGCCAAAGTTCTGAATTAAGTGGATCATTTGGTGAGAGTAAGGACGTGGACATTAGTTATACGCAAGAAAAGGCTCCCGTTGTTGGAAATGAAACTGATAAACTCAGGGTATTGCAAGAAGCACTGGAAAAAGAGAAAGTTGCTTGTGCTGCTCTTTATCTAGAGCTAGAGAAAGAGCGAGCTGCTGCTGCAAGTGCTGCTGATGAAGCGATGTCCATGATAGCACGTCTGCAAAAGGATAAAGCATCAATAGAAATGGAAGTGAGGCAGTATCAGAGGATGATGGAAGAAAAATTTGTTTATGATGAAGAGGAGATGGGTGTTCTGAAAGAGATTCTTCTTAGGAGGGAGATGGAGAACCATTTTTTGGAGAAGGAAGTTGAATCGTATAGGCAAATGAGCTTCTTAGGAATGAACGGTCTGATGCTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

222

Amino Acids

25.26

Weight (kDa)

4.98

Isoelectric Point (pI)

60.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 115 - 204 3.5e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 598
AciI CCGC 1 cut(s) 44
AclWI GGATC 1 cut(s) 262
AcsI RAATTY 2 cut(s) 24, 524
AcuI CTGAAG 1 cut(s) 29
AfiI CCNNNNNNNGG 2 cut(s) 314, 598
AgsI TTSAA 2 cut(s) 93, 614
AjiI CACGTC 2 cut(s) 277, 458
AjuI GAANNNNNNNTTGG 4 cut(s) 73, 105, 581, 613
AluBI AGCT 5 cut(s) 162, 176, 397, 413, 633
AluI AGCT 5 cut(s) 162, 176, 397, 413, 633
AlwI GGATC 1 cut(s) 262
ApeKI GCWGC 5 cut(s) 380, 413, 416, 419, 428
ApoI RAATTY 2 cut(s) 24, 524
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 33
AsuHPI GGTGA 2 cut(s) 167, 275
BbvI GCAGC 5 cut(s) 367, 400, 403, 406, 415
BccI CCATC 3 cut(s) 508, 541, 577
BfaI CTAG 3 cut(s) 36, 392, 398
BfoI RGCGCY 1 cut(s) 34
BisI GCNGC 6 cut(s) 45, 381, 414, 417, 420, 429
BlsI GCNGC 6 cut(s) 46, 382, 415, 418, 421, 430
BmgBI CACGTC 2 cut(s) 277, 458
BmiI GGNNCC 2 cut(s) 48, 306
BmsI GCATC 2 cut(s) 485, 645
BplI GAGNNNNNCTC 2 cut(s) 31, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 598
Bse1I ACTGG 3 cut(s) 25, 211, 360
BseGI GGATG 1 cut(s) 516
BseLI CCNNNNNNNGG 2 cut(s) 314, 598
BseMII CTCAG 2 cut(s) 213, 349
BseNI ACTGG 3 cut(s) 25, 211, 360
BseRI GAGGAG 1 cut(s) 557
BseXI GCAGC 5 cut(s) 367, 400, 403, 406, 415
BslI CCNNNNNNNGG 2 cut(s) 314, 598
Bsp143I GATC 1 cut(s) 254
BspACI CCGC 1 cut(s) 44
BspCNI CTCAG 2 cut(s) 214, 348
BspLI GGNNCC 2 cut(s) 48, 306
BspPI GGATC 1 cut(s) 262
BsrI ACTGG 3 cut(s) 25, 211, 360
BssMI GATC 1 cut(s) 254
Bst4CI ACNGT 1 cut(s) 650
Bst6I CTCTTC 1 cut(s) 534
BstAPI GCANNNNNTGC 1 cut(s) 428
BstC8I GCNNGC 1 cut(s) 411
BstDEI CTNAG 4 cut(s) 222, 335, 572, 637
BstF5I GGATG 1 cut(s) 516
BstH2I RGCGCY 1 cut(s) 34
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 1 cut(s) 257
BstMBI GATC 1 cut(s) 254
BstMWI GCNNNNNNNGC 5 cut(s) 380, 419, 425, 428, 437
BstV1I GCAGC 5 cut(s) 367, 400, 403, 406, 415
BtgZI GCGATG 2 cut(s) 141, 455
BtrI CACGTC 2 cut(s) 277, 458
BtsCI GGATG 1 cut(s) 516
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 1 cut(s) 411
CfoI GCGC 1 cut(s) 33
CviAII CATG 1 cut(s) 448
CviJI RGCY 9 cut(s) 47, 64, 162, 176, 221, 305, 397, 413, 633
CviKI_1 RGCY 9 cut(s) 47, 64, 162, 176, 221, 305, 397, 413, 633
DdeI CTNAG 4 cut(s) 222, 335, 572, 637
DpnI GATC 1 cut(s) 256
DpnII GATC 1 cut(s) 254
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
Eco57I CTGAAG 1 cut(s) 29
EcoRI GAATTC 1 cut(s) 24
FaeI CATG 1 cut(s) 451
FalI AAGNNNNNCTT 4 cut(s) 52, 84, 552, 584
FatI CATG 1 cut(s) 447
Fnu4HI GCNGC 6 cut(s) 45, 381, 414, 417, 420, 429
FokI GGATG 1 cut(s) 523
Fsp4HI GCNGC 6 cut(s) 45, 381, 414, 417, 420, 429
FspBI CTAG 3 cut(s) 36, 392, 398
GlaI GCGC 1 cut(s) 32
GluI GCNGC 6 cut(s) 45, 381, 414, 417, 420, 429
HaeII RGCGCY 1 cut(s) 34
HhaI GCGC 1 cut(s) 33
Hin1II CATG 1 cut(s) 451
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HinfI GANTC 2 cut(s) 565, 614
HphI GGTGA 2 cut(s) 167, 275
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 5 cut(s) 9, 244, 507, 558, 654
Hpy188III TCNNGA 2 cut(s) 50, 392
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 2 cut(s) 78, 598
HpyCH4III ACNGT 1 cut(s) 650
HpyCH4IV ACGT 2 cut(s) 276, 457
HpyCH4V TGCA 3 cut(s) 346, 422, 463
HpyF10VI GCNNNNNNNGC 5 cut(s) 380, 419, 425, 428, 437
HpyF3I CTNAG 4 cut(s) 222, 335, 572, 637
HpySE526I ACGT 2 cut(s) 276, 457
Hsp92II CATG 1 cut(s) 451
HspAI GCGC 1 cut(s) 31
Kzo9I GATC 1 cut(s) 254
LmnI GCTCC 2 cut(s) 52, 310
LpnPI CCDG 7 cut(s) 6, 63, 138, 162, 192, 322, 341
Lsp1109I GCAGC 5 cut(s) 367, 400, 403, 406, 415
LweI GCATC 2 cut(s) 485, 645
MaeI CTAG 3 cut(s) 36, 392, 398
MaeII ACGT 2 cut(s) 276, 457
MalI GATC 1 cut(s) 256
MboI GATC 1 cut(s) 254
MboII GAAGA 5 cut(s) 22, 197, 530, 551, 560
MfeI CAATTG 1 cut(s) 165
MluCI AATT 4 cut(s) 24, 165, 245, 524
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 5 cut(s) 33, 489, 501, 535, 570
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 1 cut(s) 248
MunI CAATTG 1 cut(s) 165
MwoI GCNNNNNNNGC 5 cut(s) 380, 419, 425, 428, 437
NdeII GATC 1 cut(s) 254
NlaIII CATG 1 cut(s) 451
NlaIV GGNNCC 2 cut(s) 48, 306
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 2 cut(s) 565, 614
PflMI CCANNNNNTGG 1 cut(s) 598
PkrI GCNGC 6 cut(s) 46, 382, 415, 418, 421, 430
PspN4I GGNNCC 2 cut(s) 48, 306
SaqAI TTAA 1 cut(s) 248
SatI GCNGC 6 cut(s) 45, 381, 414, 417, 420, 429
Sau3AI GATC 1 cut(s) 254
SetI ASST 8 cut(s) 157, 164, 178, 279, 399, 415, 460, 635
SfaNI GCATC 2 cut(s) 485, 645
Sse9I AATT 4 cut(s) 24, 165, 245, 524
SsiI CCGC 1 cut(s) 44
SspMI CTAG 3 cut(s) 36, 392, 398
TaaI ACNGT 1 cut(s) 650
TaiI ACGT 2 cut(s) 279, 460
TasI AATT 4 cut(s) 24, 165, 245, 524
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 2 cut(s) 565, 614
Tru1I TTAA 1 cut(s) 248
Tru9I TTAA 1 cut(s) 248
TscAI CASTG 1 cut(s) 360
TseI GCWGC 5 cut(s) 380, 413, 416, 419, 428
TspDTI ATGAA 5 cut(s) 72, 336, 450, 552, 659
TspRI CASTG 1 cut(s) 360
Van91I CCANNNNNTGG 1 cut(s) 598
XapI RAATTY 2 cut(s) 24, 524
XbaI TCTAGA 1 cut(s) 391
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 3 cut(s) 36, 392, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.