AT4G14170

pentatricopeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011997
Physical Location & Seq
Reverse (-)
8176144 .. 8178200
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G14170.1

Sequence Viewer

Length: 1434 bp
ATGCACTTACCATCTTCCTCTTCCTTGTACTCGTCTCCTACGAAGAGTCTATTCGCAATGTTCTTGAAAGCTCGAGCTTTATCAGTAACTACACAGAATCCTCCTGATCTCTTCTCGCTTCTACATCATTCTCCAAACGCTAAACATCTACGCCATCTCCACGCTCACCTTCTTCGTACATTTCTCTATTCCAACGTTGTGCTCAGCTCCAAGCTCGTCCTCGCTTATTCCAAGCTGAACCATCTCTTTCCTACTTCGCTTTCTGTCTTCTGGCACATGCCTTATCGCAACATTTTCTCTTGGAATATAATCATCGGTGAGTTCTCTAGATCGGGTTTTGCTTCCAAGTCAATTGATTTGTTTCTTCGTATGTGGCGAGAGTCTTGTGTTAGACCGGATGATTTTACGCTTCCTCTTATTCTACGAGCTTGCTCTGCGTCTAGGGAAGCGAAATCTGGAGATTTGATTCATGTTCTGTGTTTGAAACTTGGGTTTAGCTCAAGTTTGTTTGTTAGTTCGGCTTTAGTGATAATGTATGTAGATATGGGGAAACTTTTACATGCACGTAAGTTGTTCGACGATATGCCTGTGAGAGACTCTGTTCTTTATACAGCTATGTTTGGTGGGTATGTTCAGCAAGGAGAAGCTATGTTGGGTTTAGCCATGTTCAGAGAAATGGGGTATTCTGGATTTGCGCTGGATTCAGTGGTGATGGTGAGTTTACTTATGGCTTGTGGACAACTTGGAGCGTTGAAGCACGGGAAGAGCGTCCATGGATGGTGTATCAGGAGATGCTCCTGCTTGGGCTTGAATCTCGGCAATGCTATAACAGATATGTATGTAAAGTGCTCGATACTGGACTATGCGCACACTGTATTTGTAAATATGTCGAGACGAGATGTGATCTCTTGGAGCTCGCTTATTTTGGGGTATGGTTTGGATGGAGACGTGGTAATGTCTTTTAAACTCTTTGATGAGATGCTTAAGGAAGGAATTGAACCCAATGCTGTCACTTTTCTTGGTGTCTTATCAGCCTGTGCCCATGGCGGTCTTGTGGAAAAATCTTGGCTGTATTTCAGACTAATGCAGGAATATAATATAGTTCCTGAACTGAAACACTATGCTAGTGTGGCGGATTGTATGTCTCGAGCAGGTCTTCTGGAGGAAGCAGAGAAGTTTCTGGAAGATATGCCCGTGAAACCTGATGAGGCTGTTATGGGTGCGGTGTTGAGTGGGTGCAAGGTGTATGGAAATGTAGAAGTTGGGGAAAGAGTGGCGAGGGAGCTGATTCAACTTAAGCCGAGAAAAGCTAGCTACTATGTAACGTTGGCCGGTCTATATTCAGCTGCAGGTCGGTTTGATGAAGCTGAGAGTCTGAGGCAGTGGATGAAGGAGAAACAGATTTCTAAGGTTCCCGGGTGTAGCTCAATATGA

Protein Analysis

477

Amino Acids

53.25

Weight (kDa)

8.7

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 299 - 348 1.2e-10 PPR repeat family
PPR PF01535 302 - 332 7.4e-06 PPR repeat
E_motif PF20431 416 - 477 5.1e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14170 AT4G14170
fragaria_vesca FvH4_7g28451
malus_domestica MD01G1188300.v1.1
prunus_persica Prupe.2G284600_v2.0.a1
pyrus_communis pycom01g20010
rosa_chinensis RchiOBHm_Chr1g0375631 RchiOBHm_Chr1g0375671
rosa_laevigata RLG00000026661
rosa_multiflora Rmu_co8281021.1_g000001 Rmu_co8425983.1_g000001 Rmu_sc0001644.1_g000022
rosa_roxburghii Rroxscaffold_4G00282680
rosa_rugosa Rorug01G0391700
rosa_samantha Rh1AG402600 Rh1BG366800 Rh1CG379500
rosa_wichuraiana Rw1G035750 Rw1G035770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 867
Acc36I ACCTGC 2 cut(s) 1142, 1340
AciI CCGC 3 cut(s) 1047, 1133, 1223
AclI AACGTT 2 cut(s) 195, 1325
AcoI YGGCCR 1 cut(s) 1329
AfaI GTAC 2 cut(s) 29, 178
AflII CTTAAG 2 cut(s) 983, 1295
AgsI TTSAA 6 cut(s) 67, 484, 754, 811, 998, 1292
AjiI CACGTC 1 cut(s) 949
Alw21I GWGCWC 3 cut(s) 204, 851, 917
Alw26I GTCTC 5 cut(s) 39, 588, 886, 939, 1149
Ama87I CYCGRG 3 cut(s) 72, 1146, 1415
AoxI GGCC 1 cut(s) 1329
ApeKI GCWGC 1 cut(s) 1346
AspLEI GCGC 2 cut(s) 697, 868
AsuC2I CCSGG 2 cut(s) 1416, 1417
AsuHPI GGTGA 4 cut(s) 158, 329, 721, 727
AsuNHI GCTAGC 1 cut(s) 1310
AvaI CYCGRG 3 cut(s) 72, 1146, 1415
BaeGI GKGCMC 1 cut(s) 1042
BanII GRGCYC 1 cut(s) 917
BbsI GAAGAC 2 cut(s) 259, 1148
Bbv12I GWGCWC 3 cut(s) 204, 851, 917
BbvI GCAGC 1 cut(s) 1333
BccI CCATC 6 cut(s) 19, 162, 249, 706, 771, 935
BcnI CCSGG 2 cut(s) 1416, 1417
BcoDI GTCTC 5 cut(s) 39, 588, 886, 939, 1149
BfaI CTAG 4 cut(s) 327, 441, 1125, 1311
BfmI CTRYAG 1 cut(s) 1347
BfrI CTTAAG 2 cut(s) 983, 1295
BfuAI ACCTGC 2 cut(s) 1142, 1340
BisI GCNGC 1 cut(s) 1347
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 1 cut(s) 1348
Bme1390I CCNGG 2 cut(s) 1416, 1417
BmeT110I CYCGRG 3 cut(s) 72, 1146, 1415
BmgBI CACGTC 1 cut(s) 949
BmiI GGNNCC 1 cut(s) 1413
BmrFI CCNGG 2 cut(s) 1416, 1417
BmsI GCATC 2 cut(s) 782, 969
BmtI GCTAGC 1 cut(s) 1314
BpiI GAAGAC 2 cut(s) 259, 1148
BpmI CTGGAG 2 cut(s) 477, 1181
Bpu1102I GCTNAGC 1 cut(s) 203
BpuEI CTTGAG 1 cut(s) 484
BpuMI CCSGG 2 cut(s) 1416, 1417
BsaAI YACGTR 1 cut(s) 566
BsaJI CCNNGG 3 cut(s) 772, 1042, 1415
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 936, 966
Bse118I RCCGGY 1 cut(s) 1331
Bse1I ACTGG 1 cut(s) 861
Bse3DI GCAATG 2 cut(s) 63, 826
BseDI CCNNGG 3 cut(s) 772, 1042, 1415
BseGI GGATG 4 cut(s) 403, 782, 946, 1392
BseMI GCAATG 2 cut(s) 63, 826
BseMII CTCAG 3 cut(s) 217, 1359, 1367
BseNI ACTGG 1 cut(s) 861
BseSI GKGCMC 1 cut(s) 1042
BseXI GCAGC 1 cut(s) 1333
BshFI GGCC 1 cut(s) 1331
BsiHKAI GWGCWC 3 cut(s) 204, 851, 917
BsiHKCI CYCGRG 3 cut(s) 72, 1146, 1415
BsiSI CCGG 3 cut(s) 395, 1332, 1416
BsmAI GTCTC 5 cut(s) 39, 588, 886, 939, 1149
BsmBI CGTCTC 3 cut(s) 39, 886, 939
BsnI GGCC 1 cut(s) 1331
BsoBI CYCGRG 3 cut(s) 72, 1146, 1415
Bsp1286I GDGCHC 4 cut(s) 204, 851, 917, 1042
Bsp143I GATC 3 cut(s) 106, 329, 903
Bsp1720I GCTNAGC 1 cut(s) 203
Bsp19I CCATGG 2 cut(s) 772, 1042
BspACI CCGC 3 cut(s) 1047, 1133, 1223
BspANI GGCC 1 cut(s) 1331
BspCNI CTCAG 3 cut(s) 216, 1360, 1368
BspLI GGNNCC 1 cut(s) 1413
BspMAI CTGCAG 1 cut(s) 1351
BspMI ACCTGC 2 cut(s) 1142, 1340
BspOI GCTAGC 1 cut(s) 1314
BspQI GCTCTTC 1 cut(s) 758
BspTI CTTAAG 2 cut(s) 983, 1295
BsrDI GCAATG 2 cut(s) 63, 826
BsrFI RCCGGY 1 cut(s) 1331
BsrI ACTGG 1 cut(s) 861
BssAI RCCGGY 1 cut(s) 1331
BssECI CCNNGG 3 cut(s) 772, 1042, 1415
BssMI GATC 3 cut(s) 106, 329, 903
BssT1I CCWWGG 2 cut(s) 772, 1042
Bst4CI ACNGT 1 cut(s) 874
Bst6I CTCTTC 4 cut(s) 25, 38, 116, 758
BstAFI CTTAAG 2 cut(s) 983, 1295
BstBAI YACGTR 1 cut(s) 566
BstC8I GCNNGC 3 cut(s) 430, 917, 1312
BstDEI CTNAG 4 cut(s) 203, 1368, 1376, 1407
BstDSI CCRYGG 2 cut(s) 772, 1042
BstF5I GGATG 4 cut(s) 403, 782, 946, 1392
BstHHI GCGC 2 cut(s) 697, 868
BstKTI GATC 3 cut(s) 109, 332, 906
BstMAI GTCTC 5 cut(s) 39, 588, 886, 939, 1149
BstMBI GATC 3 cut(s) 106, 329, 903
BstMWI GCNNNNNNNGC 2 cut(s) 434, 1130
BstNSI RCATGY 2 cut(s) 280, 563
BstSCI CCNGG 2 cut(s) 1414, 1415
BstSFI CTRYAG 1 cut(s) 1347
BstSLI GKGCMC 1 cut(s) 1042
BstV1I GCAGC 1 cut(s) 1333
BstV2I GAAGAC 2 cut(s) 259, 1148
BsuRI GGCC 1 cut(s) 1331
BtgI CCRYGG 2 cut(s) 772, 1042
BtrI CACGTC 1 cut(s) 949
BtsCI GGATG 4 cut(s) 403, 782, 946, 1392
BtsI GCAGTG 1 cut(s) 1388
BtsIMutI CAGTG 3 cut(s) 711, 870, 1388
BveI ACCTGC 2 cut(s) 1142, 1340
Cac8I GCNNGC 3 cut(s) 430, 917, 1312
CfoI GCGC 2 cut(s) 697, 868
Cfr10I RCCGGY 1 cut(s) 1331
Cfr9I CCCGGG 1 cut(s) 1415
CseI GACGC 2 cut(s) 426, 757
Csp6I GTAC 2 cut(s) 28, 177
CviAII CATG 6 cut(s) 277, 470, 560, 664, 773, 1043
CviQI GTAC 2 cut(s) 28, 177
DdeI CTNAG 4 cut(s) 203, 1368, 1376, 1407
DpnI GATC 3 cut(s) 108, 331, 905
DpnII GATC 3 cut(s) 106, 329, 903
DraI TTTAAA 1 cut(s) 964
EaeI YGGCCR 1 cut(s) 1329
Eam1104I CTCTTC 4 cut(s) 25, 38, 116, 758
EarI CTCTTC 4 cut(s) 25, 38, 116, 758
EciI GGCGGA 1 cut(s) 1148
Ecl136II GAGCTC 1 cut(s) 915
Eco130I CCWWGG 2 cut(s) 772, 1042
Eco24I GRGCYC 1 cut(s) 917
Eco53kI GAGCTC 1 cut(s) 915
Eco88I CYCGRG 3 cut(s) 72, 1146, 1415
EcoICRI GAGCTC 1 cut(s) 915
EcoT14I CCWWGG 2 cut(s) 772, 1042
EcoT38I GRGCYC 1 cut(s) 917
ErhI CCWWGG 2 cut(s) 772, 1042
Esp3I CGTCTC 3 cut(s) 39, 886, 939
FaeI CATG 6 cut(s) 280, 473, 563, 667, 776, 1046
FatI CATG 6 cut(s) 276, 469, 559, 663, 772, 1042
Fnu4HI GCNGC 1 cut(s) 1347
FokI GGATG 4 cut(s) 410, 789, 953, 1399
FriOI GRGCYC 1 cut(s) 917
Fsp4HI GCNGC 1 cut(s) 1347
FspAI RTGCGCAY 1 cut(s) 867
FspBI CTAG 4 cut(s) 327, 441, 1125, 1311
FspI TGCGCA 1 cut(s) 867
GlaI GCGC 2 cut(s) 696, 867
GluI GCNGC 1 cut(s) 1347
GsuI CTGGAG 2 cut(s) 477, 1181
HaeIII GGCC 1 cut(s) 1331
HapII CCGG 3 cut(s) 395, 1332, 1416
HgaI GACGC 2 cut(s) 426, 757
HhaI GCGC 2 cut(s) 697, 868
Hin1II CATG 6 cut(s) 280, 473, 563, 667, 776, 1046
Hin6I GCGC 2 cut(s) 695, 866
HinP1I GCGC 2 cut(s) 695, 866
HinfI GANTC 9 cut(s) 46, 97, 380, 466, 596, 701, 811, 1288, 1372
HpaII CCGG 3 cut(s) 395, 1332, 1416
HphI GGTGA 4 cut(s) 158, 329, 721, 727
Hpy166II GTNNAC 2 cut(s) 722, 737
Hpy188I TCNGA 3 cut(s) 671, 1079, 1377
Hpy8I GTNNAC 2 cut(s) 722, 737
Hpy99I CGWCG 1 cut(s) 581
HpyAV CCTTC 3 cut(s) 179, 983, 1384
HpyCH4III ACNGT 1 cut(s) 874
HpyCH4IV ACGT 4 cut(s) 195, 565, 948, 1325
HpyCH4V TGCA 5 cut(s) 4, 563, 1087, 1239, 1349
HpyF10VI GCNNNNNNNGC 2 cut(s) 434, 1130
HpyF3I CTNAG 4 cut(s) 203, 1368, 1376, 1407
HpySE526I ACGT 4 cut(s) 195, 565, 948, 1325
Hsp92II CATG 6 cut(s) 280, 473, 563, 667, 776, 1046
HspAI GCGC 2 cut(s) 695, 866
Kzo9I GATC 3 cut(s) 106, 329, 903
LguI GCTCTTC 1 cut(s) 758
LmnI GCTCC 5 cut(s) 212, 746, 800, 912, 1282
Lsp1109I GCAGC 1 cut(s) 1333
LweI GCATC 2 cut(s) 782, 969
MaeI CTAG 4 cut(s) 327, 441, 1125, 1311
MaeII ACGT 4 cut(s) 195, 565, 948, 1325
MaeIII GTNAC 3 cut(s) 85, 1009, 1321
MalI GATC 3 cut(s) 108, 331, 905
MboI GATC 3 cut(s) 106, 329, 903
MfeI CAATTG 1 cut(s) 351
MhlI GDGCHC 4 cut(s) 204, 851, 917, 1042
MluCI AATT 2 cut(s) 351, 993
MlyI GAGTC 4 cut(s) 55, 389, 590, 1381
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 8 cut(s) 28, 111, 230, 423, 1156, 1201, 1272, 1371
MseI TTAA 3 cut(s) 963, 984, 1296
MspA1I CMGCKG 1 cut(s) 1346
MspCI CTTAAG 2 cut(s) 983, 1295
MspI CCGG 3 cut(s) 395, 1332, 1416
MspR9I CCNGG 2 cut(s) 1416, 1417
MunI CAATTG 1 cut(s) 351
MwoI GCNNNNNNNGC 2 cut(s) 434, 1130
NciI CCSGG 2 cut(s) 1416, 1417
NcoI CCATGG 2 cut(s) 772, 1042
NdeII GATC 3 cut(s) 106, 329, 903
NheI GCTAGC 1 cut(s) 1310
NlaIII CATG 6 cut(s) 280, 473, 563, 667, 776, 1046
NlaIV GGNNCC 1 cut(s) 1413
NmeAIII GCCGAG 2 cut(s) 795, 1326
NmuCI GTSAC 1 cut(s) 1009
NsbI TGCGCA 1 cut(s) 867
NspI RCATGY 2 cut(s) 280, 563
PaeR7I CTCGAG 2 cut(s) 72, 1146
PciSI GCTCTTC 1 cut(s) 758
PcsI WCGNNNNNNNCGW 3 cut(s) 38, 373, 765
PfeI GAWTC 5 cut(s) 97, 466, 701, 811, 1288
PkrI GCNGC 1 cut(s) 1348
PleI GAGTC 4 cut(s) 54, 388, 590, 1380
PpsI GAGTC 4 cut(s) 54, 388, 590, 1380
Ppu21I YACGTR 1 cut(s) 566
Psp124BI GAGCTC 1 cut(s) 917
Psp1406I AACGTT 2 cut(s) 195, 1325
PspN4I GGNNCC 1 cut(s) 1413
PspXI VCTCGAGB 1 cut(s) 72
PstI CTGCAG 1 cut(s) 1351
PvuII CAGCTG 1 cut(s) 1346
RsaI GTAC 2 cut(s) 29, 178
RsaNI GTAC 2 cut(s) 28, 177
SacI GAGCTC 1 cut(s) 917
SapI GCTCTTC 1 cut(s) 758
SaqAI TTAA 3 cut(s) 963, 984, 1296
SatI GCNGC 1 cut(s) 1347
Sau3AI GATC 3 cut(s) 106, 329, 903
SchI GAGTC 4 cut(s) 55, 389, 590, 1381
ScrFI CCNGG 2 cut(s) 1416, 1417
SduI GDGCHC 4 cut(s) 204, 851, 917, 1042
SfaNI GCATC 2 cut(s) 782, 969
SfcI CTRYAG 1 cut(s) 1347
Sfr274I CTCGAG 2 cut(s) 72, 1146
SlaI CTCGAG 2 cut(s) 72, 1146
SmaI CCCGGG 1 cut(s) 1417
SmlI CTYRAG 5 cut(s) 72, 499, 983, 1146, 1295
SmoI CTYRAG 5 cut(s) 72, 499, 983, 1146, 1295
Sse9I AATT 2 cut(s) 351, 993
SsiI CCGC 3 cut(s) 1047, 1133, 1223
SspMI CTAG 4 cut(s) 327, 441, 1125, 1311
SstI GAGCTC 1 cut(s) 917
StyD4I CCNGG 2 cut(s) 1414, 1415
StyI CCWWGG 2 cut(s) 772, 1042
TaaI ACNGT 1 cut(s) 874
TaiI ACGT 4 cut(s) 198, 568, 951, 1328
TaqI TCGA 5 cut(s) 73, 576, 851, 890, 1147
TasI AATT 2 cut(s) 351, 993
TatI WGTACW 1 cut(s) 27
TfiI GAWTC 5 cut(s) 97, 466, 701, 811, 1288
Tru1I TTAA 3 cut(s) 963, 984, 1296
Tru9I TTAA 3 cut(s) 963, 984, 1296
TscAI CASTG 3 cut(s) 711, 877, 1388
TseFI GTSAC 1 cut(s) 1009
TseI GCWGC 1 cut(s) 1346
Tsp45I GTSAC 1 cut(s) 1009
TspDTI ATGAA 3 cut(s) 458, 1377, 1403
TspMI CCCGGG 1 cut(s) 1415
TspRI CASTG 3 cut(s) 711, 877, 1388
Vha464I CTTAAG 2 cut(s) 983, 1295
XbaI TCTAGA 1 cut(s) 326
XceI RCATGY 2 cut(s) 280, 563
XhoI CTCGAG 2 cut(s) 72, 1146
XmaI CCCGGG 1 cut(s) 1415
XspI CTAG 4 cut(s) 327, 441, 1125, 1311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.