Rmu_sc0001644.1_g000022

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001644.1
OG0011997
Physical Location & Seq
Forward (+)
109383 .. 111283
1901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001644.1_g000022.1.cds

Sequence Viewer

Length: 1713 bp
atgtcaagtcttagaaccacactcttacacttgctcgaagaatgcaagaacatgagagagctgaaacaaatccacacccacatcataaagtccccatttttactcaaacaagaccaaacctttctcatcactcgtctcctattcttctgcgctctatccgattcgggttctcagacctacgccaccgcagttttccgggtcataaagaacccgactctctacgtctacaacatcatgatcagagcatacgcgtgtagaaagaccagtgctttggataaacctgcttcctttcggtcgttggtgttgtataagcaaatggttggtaatgggattgggcccaattgcctcacttttccgtttcttgtgaaggaatgcgcggcgaggagtgacggcggcgctggccggagcgttcatggccaagtggttaagtacgggtgtgaagatgatgtgtttgttcgaaactcgttgatgaatatgtactctgtttgtgggtctttgagttgcgcacgcaaggtgttcaatgaaatgcctgagagagatgtggtttcgtggaacttgatgattaaggggtgtttgagaagtggggaacttgatgaggcgatgggtgtgtttgggaagatgaagcagaggagtatttttaggtgcgttcatggccaagtggttaagtacgggtgcgaagatgatgtgtttgttcgaaactcgttgatgaatatgtactctgtttgtgggtctttgagttgcgcacgcaaggtgttcgatgaaatgcctgagagagatgtggtttcgtggaacttggtgattaagggggtggtcggccgaatgaggcatttggaactttttcatgaaatgcaggctgcgggtggtgttagaccggataagattacgattgctagtgtccttacggcttgtgctcatcttggcgcggttgatcacgggacatgggtgcatgggtacctgaggagaagtgggctagagagtgatgtggttattggaacagctctggttgacatgtatggtaaatgtggatgtgtggaaaaagcttatgaggtctttaaggaaatgccgaagaaagatactttggcatggacagctatgatttcggtttttgctctccatgggtttggcaatgaggcttttgatctttttgaaaagatggaagcaactggggttgagccgaatcatgtgacatttgttgggttgttgtcagcttgtgctcattccggtttagtagagaaaggtcgctggtgttttgatgtgatgagacgtgtttacttaattgaacctcagctttatcattatgcatgcatggttgatatacttagcagagctgggctaattgaagaggcggagaggttgataagaagcatgccaatgaagccagatgtatttgtttggggtgcattacttggaggttgtcaaatacatggaaaggttgagataggagaactggtggcccgtcatttaattgatatggaacctctgaaccatgctttttatgtcaacttgtgcgatatatatgccaaagctggtagatttaatgatgtgaaaacaattaaatcactaatgaaagaaagagggataaaaaaggaagttgcaggctgtagcctgattgaagtcgatggagttgttcttgaattctcagtcagaggatcccctgatgttgtaatggatgaagtattatgcgtcttgagtcagttgaatagtgaggcctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

570

Amino Acids

63.68

Weight (kDa)

7.94

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14170 AT4G14170
fragaria_vesca FvH4_7g28451
malus_domestica MD01G1188300.v1.1
prunus_persica Prupe.2G284600_v2.0.a1
pyrus_communis pycom01g20010
rosa_chinensis RchiOBHm_Chr1g0375631 RchiOBHm_Chr1g0375671
rosa_laevigata RLG00000026661
rosa_multiflora Rmu_co8281021.1_g000001 Rmu_co8425983.1_g000001 Rmu_sc0001644.1_g000022
rosa_roxburghii Rroxscaffold_4G00282680
rosa_rugosa Rorug01G0391700
rosa_samantha Rh1AG402600 Rh1BG366800 Rh1CG379500
rosa_wichuraiana Rw1G035750 Rw1G035770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 505, 742
Acc36I ACCTGC 1 cut(s) 289
Acc65I GGTACC 1 cut(s) 951
AccB1I GGYRCC 1 cut(s) 951
AccI GTMKAC 1 cut(s) 225
AccII CGCG 3 cut(s) 251, 377, 923
AciI CCGC 6 cut(s) 186, 377, 393, 857, 923, 1346
AclWI GGATC 2 cut(s) 1644, 1657
AcoI YGGCCR 4 cut(s) 400, 415, 652, 814
AcsI RAATTY 1 cut(s) 1634
AfaI GTAC 5 cut(s) 431, 479, 668, 716, 953
AflIII ACRYGT 3 cut(s) 249, 1008, 1264
AgsI TTSAA 7 cut(s) 520, 1148, 1280, 1340, 1613, 1634, 1699
AjiI CACGTC 1 cut(s) 1265
AjuI GAANNNNNNNTTGG 2 cut(s) 1061, 1093
AluBI AGCT 8 cut(s) 61, 998, 1040, 1091, 1208, 1288, 1328, 1526
AluI AGCT 8 cut(s) 61, 998, 1040, 1091, 1208, 1288, 1328, 1526
Alw21I GWGCWC 2 cut(s) 913, 1216
Alw26I GTCTC 2 cut(s) 140, 1255
AlwI GGATC 2 cut(s) 1644, 1657
AoxI GGCC 7 cut(s) 335, 400, 415, 652, 814, 1452, 1707
ApaI GGGCCC 1 cut(s) 339
ApeKI GCWGC 1 cut(s) 854
ApoI RAATTY 1 cut(s) 1634
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 1 cut(s) 837
Asp718I GGTACC 1 cut(s) 951
AspLEI GCGC 6 cut(s) 152, 377, 398, 506, 743, 923
AspS9I GGNCC 3 cut(s) 335, 336, 1453
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 808
AsuII TTCGAA 2 cut(s) 457, 694
AxyI CCTNAGG 1 cut(s) 956
BaeGI GKGCMC 1 cut(s) 339
BalI TGGCCA 2 cut(s) 417, 654
BamHI GGATCC 1 cut(s) 1649
BanI GGYRCC 1 cut(s) 951
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 2 cut(s) 913, 1216
BbvCI CCTCAGC 1 cut(s) 1284
BbvI GCAGC 1 cut(s) 841
BccI CCATC 3 cut(s) 595, 1147, 1613
BceAI ACGGC 2 cut(s) 406, 918
BcgI CGANNNNNNTGC 4 cut(s) 736, 770, 1499, 1533
BclI TGATCA 2 cut(s) 237, 928
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 2 cut(s) 140, 1255
BfaI CTAG 3 cut(s) 891, 971, 1711
BfmI CTRYAG 1 cut(s) 1600
BfoI RGCGCY 1 cut(s) 399
BfuAI ACCTGC 1 cut(s) 289
BisI GCNGC 3 cut(s) 378, 394, 855
BlsI GCNGC 3 cut(s) 379, 395, 856
Bme1390I CCNGG 1 cut(s) 197
BmgBI CACGTC 1 cut(s) 1265
BmgT120I GGNCC 3 cut(s) 335, 336, 1453
BmiI GGNNCC 4 cut(s) 337, 953, 1476, 1651
BmrFI CCNGG 1 cut(s) 197
BmrI ACTGGG 1 cut(s) 1173
BmuI ACTGGG 1 cut(s) 1173
Bpu10I CCTNAGC 1 cut(s) 1284
Bpu14I TTCGAA 2 cut(s) 457, 694
BpuEI CTTGAG 1 cut(s) 1708
BpuMI CCSGG 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 1114
BsaWI WCCGGW 2 cut(s) 871, 1220
BsaXI ACNNNNNCTCC 2 cut(s) 1434, 1464
Bse1I ACTGG 3 cut(s) 264, 1168, 1452
Bse21I CCTNAGG 1 cut(s) 956
Bse3DI GCAATG 1 cut(s) 1132
BseDI CCNNGG 1 cut(s) 1114
BseGI GGATG 2 cut(s) 1031, 1675
BseMI GCAATG 1 cut(s) 1132
BseMII CTCAG 6 cut(s) 185, 522, 759, 947, 1298, 1653
BseNI ACTGG 3 cut(s) 264, 1168, 1452
BseRI GAGGAG 3 cut(s) 397, 643, 973
BseSI GKGCMC 1 cut(s) 339
BseX3I CGGCCG 1 cut(s) 814
BseXI GCAGC 1 cut(s) 841
BseYI CCCAGC 1 cut(s) 1328
Bsh1236I CGCG 3 cut(s) 251, 377, 923
Bsh1285I CGRYCG 2 cut(s) 296, 817
BshFI GGCC 7 cut(s) 337, 402, 417, 654, 816, 1454, 1709
BshNI GGYRCC 1 cut(s) 951
BsiEI CGRYCG 2 cut(s) 296, 817
BsiHKAI GWGCWC 2 cut(s) 913, 1216
BsiSI CCGG 4 cut(s) 196, 403, 872, 1221
BslFI GGGAC 2 cut(s) 76, 949
BsmAI GTCTC 2 cut(s) 140, 1255
BsmBI CGTCTC 2 cut(s) 140, 1255
BsmFI GGGAC 2 cut(s) 76, 949
BsmI GAATGC 2 cut(s) 47, 377
BsnI GGCC 7 cut(s) 337, 402, 417, 654, 816, 1454, 1709
Bsp119I TTCGAA 2 cut(s) 457, 694
Bsp120I GGGCCC 1 cut(s) 335
Bsp1286I GDGCHC 3 cut(s) 339, 913, 1216
Bsp143I GATC 4 cut(s) 237, 928, 1138, 1649
Bsp19I CCATGG 1 cut(s) 1114
BspACI CCGC 6 cut(s) 186, 377, 393, 857, 923, 1346
BspANI GGCC 7 cut(s) 337, 402, 417, 654, 816, 1454, 1709
BspCNI CTCAG 6 cut(s) 184, 523, 760, 948, 1297, 1652
BspFNI CGCG 3 cut(s) 251, 377, 923
BspHI TCATGA 2 cut(s) 234, 841
BspLI GGNNCC 4 cut(s) 337, 953, 1476, 1651
BspMI ACCTGC 1 cut(s) 289
BspPI GGATC 2 cut(s) 1644, 1657
BspT104I TTCGAA 2 cut(s) 457, 694
BspT107I GGYRCC 1 cut(s) 951
BsrDI GCAATG 1 cut(s) 1132
BsrI ACTGG 3 cut(s) 264, 1168, 1452
BssECI CCNNGG 1 cut(s) 1114
BssMI GATC 4 cut(s) 237, 928, 1138, 1649
BssT1I CCWWGG 1 cut(s) 1114
Bst6I CTCTTC 1 cut(s) 1335
BstBI TTCGAA 2 cut(s) 457, 694
BstC8I GCNNGC 7 cut(s) 400, 508, 745, 852, 1303, 1367, 1597
BstDEI CTNAG 8 cut(s) 11, 171, 531, 768, 956, 1284, 1319, 1639
BstDSI CCRYGG 1 cut(s) 1114
BstF5I GGATG 2 cut(s) 1031, 1675
BstFNI CGCG 3 cut(s) 251, 377, 923
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 6 cut(s) 152, 377, 398, 506, 743, 923
BstKTI GATC 4 cut(s) 240, 931, 1141, 1652
BstMAI GTCTC 2 cut(s) 140, 1255
BstMBI GATC 4 cut(s) 237, 928, 1138, 1649
BstMCI CGRYCG 2 cut(s) 296, 817
BstMWI GCNNNNNNNGC 5 cut(s) 399, 414, 651, 1088, 1375
BstNSI RCATGY 3 cut(s) 1012, 1305, 1369
BstSCI CCNGG 1 cut(s) 195
BstSFI CTRYAG 1 cut(s) 1600
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 3 cut(s) 251, 377, 923
BstV1I GCAGC 1 cut(s) 841
BstX2I RGATCY 1 cut(s) 1649
BstXI CCANNNNNNTGG 2 cut(s) 271, 1121
BstYI RGATCY 1 cut(s) 1649
BstZI CGGCCG 1 cut(s) 814
Bsu36I CCTNAGG 1 cut(s) 956
BsuRI GGCC 7 cut(s) 337, 402, 417, 654, 816, 1454, 1709
BtgI CCRYGG 1 cut(s) 1114
BtgZI GCGATG 1 cut(s) 614
BtrI CACGTC 1 cut(s) 1265
BtsCI GGATG 2 cut(s) 1031, 1675
BtsIMutI CAGTG 1 cut(s) 271
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 7 cut(s) 400, 508, 745, 852, 1303, 1367, 1597
CciI TCATGA 2 cut(s) 234, 841
CfoI GCGC 6 cut(s) 152, 377, 398, 506, 743, 923
Cfr13I GGNCC 3 cut(s) 335, 336, 1453
CseI GACGC 1 cut(s) 1672
Csp6I GTAC 5 cut(s) 430, 478, 667, 715, 952
CviQI GTAC 5 cut(s) 430, 478, 667, 715, 952
DdeI CTNAG 8 cut(s) 11, 171, 531, 768, 956, 1284, 1319, 1639
DpnI GATC 4 cut(s) 239, 930, 1140, 1651
DpnII GATC 4 cut(s) 237, 928, 1138, 1649
EaeI YGGCCR 4 cut(s) 400, 415, 652, 814
EagI CGGCCG 1 cut(s) 814
Eam1104I CTCTTC 1 cut(s) 1335
EarI CTCTTC 1 cut(s) 1335
EciI GGCGGA 1 cut(s) 1361
EclXI CGGCCG 1 cut(s) 814
Eco130I CCWWGG 1 cut(s) 1114
Eco147I AGGCCT 1 cut(s) 1709
Eco24I GRGCYC 1 cut(s) 339
Eco52I CGGCCG 1 cut(s) 814
Eco81I CCTNAGG 1 cut(s) 956
EcoRI GAATTC 1 cut(s) 1634
EcoT14I CCWWGG 1 cut(s) 1114
EcoT22I ATGCAT 2 cut(s) 1303, 1307
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 1114
Esp3I CGTCTC 2 cut(s) 140, 1255
FaqI GGGAC 2 cut(s) 76, 949
FauI CCCGC 1 cut(s) 850
FbaI TGATCA 2 cut(s) 237, 928
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 3 cut(s) 378, 394, 855
FokI GGATG 2 cut(s) 1038, 1682
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 3 cut(s) 378, 394, 855
FspBI CTAG 3 cut(s) 891, 971, 1711
FspI TGCGCA 2 cut(s) 505, 742
GlaI GCGC 6 cut(s) 151, 376, 397, 505, 742, 922
GluI GCNGC 3 cut(s) 378, 394, 855
GsaI CCCAGC 1 cut(s) 1332
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 7 cut(s) 337, 402, 417, 654, 816, 1454, 1709
HapII CCGG 4 cut(s) 196, 403, 872, 1221
HgaI GACGC 1 cut(s) 1672
HhaI GCGC 6 cut(s) 152, 377, 398, 506, 743, 923
Hin6I GCGC 6 cut(s) 150, 375, 396, 504, 741, 921
HinP1I GCGC 6 cut(s) 150, 375, 396, 504, 741, 921
HincII GTYRAC 2 cut(s) 1006, 1501
HindII GTYRAC 2 cut(s) 1006, 1501
HindIII AAGCTT 1 cut(s) 1038
HinfI GANTC 4 cut(s) 161, 214, 1177, 1690
HpaII CCGG 4 cut(s) 196, 403, 872, 1221
HphI GGTGA 1 cut(s) 808
Hpy166II GTNNAC 4 cut(s) 226, 1006, 1270, 1501
Hpy188I TCNGA 5 cut(s) 160, 174, 242, 1482, 1646
Hpy188III TCNNGA 4 cut(s) 235, 842, 1631, 1687
Hpy8I GTNNAC 4 cut(s) 226, 1006, 1270, 1501
HpyAV CCTTC 1 cut(s) 361
HpyCH4IV ACGT 2 cut(s) 222, 1264
HpyCH4V TGCA 7 cut(s) 45, 850, 946, 1301, 1305, 1400, 1595
HpyF10VI GCNNNNNNNGC 5 cut(s) 399, 414, 651, 1088, 1375
HpyF3I CTNAG 8 cut(s) 11, 171, 531, 768, 956, 1284, 1319, 1639
HpySE526I ACGT 2 cut(s) 222, 1264
HspAI GCGC 6 cut(s) 150, 375, 396, 504, 741, 921
KpnI GGTACC 1 cut(s) 955
Ksp22I TGATCA 2 cut(s) 237, 928
Kzo9I GATC 4 cut(s) 237, 928, 1138, 1649
LmnI GCTCC 1 cut(s) 405
Lsp1109I GCAGC 1 cut(s) 841
MaeI CTAG 3 cut(s) 891, 971, 1711
MaeII ACGT 2 cut(s) 222, 1264
MaeIII GTNAC 2 cut(s) 386, 1183
MalI GATC 4 cut(s) 239, 930, 1140, 1651
MboI GATC 4 cut(s) 237, 928, 1138, 1649
MboII GAAGA 7 cut(s) 50, 136, 452, 628, 689, 1078, 1352
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 1 cut(s) 1649
MhlI GDGCHC 3 cut(s) 339, 913, 1216
MlsI TGGCCA 2 cut(s) 417, 654
MluCI AATT 6 cut(s) 340, 1275, 1335, 1464, 1551, 1634
MluI ACGCGT 1 cut(s) 249
MluNI TGGCCA 2 cut(s) 417, 654
MlyI GAGTC 2 cut(s) 208, 1699
Mox20I TGGCCA 2 cut(s) 417, 654
Mph1103I ATGCAT 2 cut(s) 1303, 1307
MroXI GAANNNNTTC 1 cut(s) 837
MscI TGGCCA 2 cut(s) 417, 654
MseI TTAA 9 cut(s) 426, 564, 663, 801, 1053, 1274, 1463, 1536, 1554
MslI CAYNNNNRTG 2 cut(s) 250, 1370
Msp20I TGGCCA 2 cut(s) 417, 654
MspI CCGG 4 cut(s) 196, 403, 872, 1221
MspR9I CCNGG 1 cut(s) 197
MunI CAATTG 1 cut(s) 340
Mva1269I GAATGC 2 cut(s) 47, 377
MvnI CGCG 3 cut(s) 251, 377, 923
MwoI GCNNNNNNNGC 5 cut(s) 399, 414, 651, 1088, 1375
NciI CCSGG 1 cut(s) 197
NcoI CCATGG 1 cut(s) 1114
NdeII GATC 4 cut(s) 237, 928, 1138, 1649
NlaIV GGNNCC 4 cut(s) 337, 953, 1476, 1651
NmuCI GTSAC 2 cut(s) 386, 1183
NsbI TGCGCA 2 cut(s) 505, 742
NsiI ATGCAT 2 cut(s) 1303, 1307
NspI RCATGY 3 cut(s) 1012, 1305, 1369
NspV TTCGAA 2 cut(s) 457, 694
PaeI GCATGC 2 cut(s) 1305, 1369
PagI TCATGA 2 cut(s) 234, 841
PceI AGGCCT 1 cut(s) 1709
PciI ACATGT 1 cut(s) 1008
PctI GAATGC 2 cut(s) 47, 377
PdmI GAANNNNTTC 1 cut(s) 837
PfeI GAWTC 2 cut(s) 161, 1177
PkrI GCNGC 3 cut(s) 379, 395, 856
PleI GAGTC 2 cut(s) 208, 1698
PpsI GAGTC 2 cut(s) 208, 1698
PscI ACATGT 1 cut(s) 1008
PspFI CCCAGC 1 cut(s) 1328
PspN4I GGNNCC 4 cut(s) 337, 953, 1476, 1651
PspOMI GGGCCC 1 cut(s) 335
PspPI GGNCC 3 cut(s) 335, 336, 1453
PsuI RGATCY 1 cut(s) 1649
RsaI GTAC 5 cut(s) 431, 479, 668, 716, 953
RsaNI GTAC 5 cut(s) 430, 478, 667, 715, 952
RseI CAYNNNNRTG 2 cut(s) 250, 1370
SaqAI TTAA 9 cut(s) 426, 564, 663, 801, 1053, 1274, 1463, 1536, 1554
SatI GCNGC 3 cut(s) 378, 394, 855
Sau3AI GATC 4 cut(s) 237, 928, 1138, 1649
Sau96I GGNCC 3 cut(s) 335, 336, 1453
SchI GAGTC 2 cut(s) 208, 1699
ScrFI CCNGG 1 cut(s) 197
SduI GDGCHC 3 cut(s) 339, 913, 1216
SfcI CTRYAG 1 cut(s) 1600
SfuI TTCGAA 2 cut(s) 457, 694
SmiMI CAYNNNNRTG 2 cut(s) 250, 1370
SmlI CTYRAG 1 cut(s) 1687
SmoI CTYRAG 1 cut(s) 1687
SphI GCATGC 2 cut(s) 1305, 1369
Sse9I AATT 6 cut(s) 340, 1275, 1335, 1464, 1551, 1634
SseBI AGGCCT 1 cut(s) 1709
SsiI CCGC 6 cut(s) 186, 377, 393, 857, 923, 1346
SspMI CTAG 3 cut(s) 891, 971, 1711
StuI AGGCCT 1 cut(s) 1709
StyD4I CCNGG 1 cut(s) 195
StyI CCWWGG 1 cut(s) 1114
TaiI ACGT 2 cut(s) 225, 1267
TaqI TCGA 5 cut(s) 36, 457, 694, 756, 1617
TaqII GACCGA 1 cut(s) 282
TasI AATT 6 cut(s) 340, 1275, 1335, 1464, 1551, 1634
TatI WGTACW 2 cut(s) 477, 714
TauI GCSGC 2 cut(s) 380, 396
TfiI GAWTC 2 cut(s) 161, 1177
Tru1I TTAA 9 cut(s) 426, 564, 663, 801, 1053, 1274, 1463, 1536, 1554
Tru9I TTAA 9 cut(s) 426, 564, 663, 801, 1053, 1274, 1463, 1536, 1554
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 2 cut(s) 386, 1183
TseI GCWGC 1 cut(s) 854
Tsp45I GTSAC 2 cut(s) 386, 1183
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 1 cut(s) 271
XapI RAATTY 1 cut(s) 1634
XceI RCATGY 3 cut(s) 1012, 1305, 1369
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 837
XspI CTAG 3 cut(s) 891, 971, 1711
Zsp2I ATGCAT 2 cut(s) 1303, 1307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.