MD01G1188300.v1.1

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011997
Physical Location & Seq
Forward (+)
28685533 .. 28687714
2182 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1188300.v1.1.491

Sequence Viewer

Length: 1635 bp
ATGATTGAAACCCAAATGGCAAGTGTTAGGAGCACACTACTACATTTGCTCCATGAATGCAAGAACATGAGAGAGCTTAAACAAATCCATACCCAAATCATAAAGTCTCCACTTTTACCCAAAGATGACCAAAATTTTCTCATTACTCGTCTCCTTTTCTTCTCGGCCATTTCTGATTCGGGTTCTCATGGCTACGCCATCGATGTTTTTCGAGCCATAGAGAACCCGAATCTCTACGTGTATAACGTCATGATCAGAACATATGCGTGTAGAAAGATCGGCGGCAGGGATGAAGCTCTTTCTTTTGGGTCCTTGTTGCTGTATAAGCAGATGCTCTGTGATGGGATTTCGCCGAATTGTCTCACTTTTCCGTTCCTTGTGAAAGAATGCACGAGCAGGGTTGATGGCAGCGCAGGAAGGAGCATCCATGGTCAAGCCGTTAAGTATGGGTTGGACAGTGATGTTTTTGTTCAGAATTCTTTGATGAGTATGTATTCTGAGTGCGGGTTTTTTAGTAGTGCACGTAAACTGTTTGATGAAACGCCGGGGAGGGATGTTGTCTCTTGGAACTCGATGATTAAGGGGTATTTGAAAAGTGGGAGTCTTGATGAGGCATTGAAGCTGTTTCGGAAGATGAAGAAGAGAAACATTTTTACTTGGAATTCCGTGATCACAGGGTTTGTTCAAGGTGGACGACCAAAGCAGGCCTTGGAACTTTTTCACGAAATGCAGATCACCGGTGGTGATATTGTTAGACCGGATAAGATTACAGTTGCTAGTGTGCTAGCAGCCTGTGCTCATCTTGGTGCAATTGATCATGGGATATGGGTTCATGGGTATCTGAGGAGAAGTGGTTTGGACAGTGATGTGGTTATCGGGACAGCGCTGGTTGACATGTATGGTAAGTGTGGATTTGTGGACAAAGCATATGAGGTATTCCAGGAAATGCCGAAGAAGGATACTCTGGCATGGACAGCTATGATTTCAGTGATGGCTCTCCATGGGTTAGGTAATGAGGCTTTTGGTCTTTTTAAACAGATGGAAACAGCCGGGGTGAAGCCGAACCATGTGACATTTGTTGCGCTATTTTCAGCTTGTGCTCATTCTGGTTTTGTAGAGAAAGGTCGCTGGTGTTTTGATGCAATGAAGCATGTTTACTTAATTGAACCACAGCTCTATCATTATGCTTGCATGGTTGATATACTCAGCCGTGCCGGGCTGATTGAAGAGGCAGAACAGTGTATAAGAAGTATGCCAATGAAACCAGACACATTTGTTTGGGGTGCATTACTTGGAGGCTGTCAAATACATGGAAAGGTGGAGTTAGGAGCAAGGGTGGCACAGTATTTAATTGATTTGGAGCCTCTGAACCATGCTTTTTATGTGAACTTGTGCGATATATATGCCAAAGCTTGTAGATTTAATGACGTAAAGCGAATCAAGTCATTAATGAAAGAAAGAGGTATAAAAAAGGAAGTCCCGGGCTGTAGCATGATTGAAATCAATGGGTTTGTTCTTGAATTCTCCGTGAGAGGATCACCAAATGTCGAAATGGAGGGATTATTGTGGATCTTGAATCAGTTGAATAACGATATATCTAGAGACAATGACACACGCTTCAGGGAATCACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

545

Amino Acids

61.1

Weight (kDa)

8.06

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 184 - 217 3.1e-11 PPR repeat family
PPR_1 PF12854 185 - 213 1.4e-07 PPR repeat
PPR PF01535 187 - 217 7.6e-11 PPR repeat
PPR_2 PF13041 215 - 267 5.7e-08 PPR repeat family
PPR PF01535 218 - 244 7.8e-07 PPR repeat
PPR_2 PF13041 320 - 368 1.6e-10 PPR repeat family
E_motif PF20431 437 - 500 1.2e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14170 AT4G14170
fragaria_vesca FvH4_7g28451
malus_domestica MD01G1188300.v1.1
prunus_persica Prupe.2G284600_v2.0.a1
pyrus_communis pycom01g20010
rosa_chinensis RchiOBHm_Chr1g0375631 RchiOBHm_Chr1g0375671
rosa_laevigata RLG00000026661
rosa_multiflora Rmu_co8281021.1_g000001 Rmu_co8425983.1_g000001 Rmu_sc0001644.1_g000022
rosa_roxburghii Rroxscaffold_4G00282680
rosa_rugosa Rorug01G0391700
rosa_samantha Rh1AG402600 Rh1BG366800 Rh1CG379500
rosa_wichuraiana Rw1G035750 Rw1G035770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 282, 504
AclWI GGATC 2 cut(s) 1543, 1577
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 4 cut(s) 133, 475, 661, 1520
AcuI CTGAAG 1 cut(s) 1603
AfeI AGCGCT 1 cut(s) 885
AflIII ACRYGT 2 cut(s) 237, 894
AgeI ACCGGT 1 cut(s) 737
AjnI CCWGG 1 cut(s) 939
AluBI AGCT 7 cut(s) 76, 296, 622, 977, 1094, 1174, 1412
AluI AGCT 7 cut(s) 76, 296, 622, 977, 1094, 1174, 1412
Alw21I GWGCWC 4 cut(s) 35, 523, 799, 1102
Alw26I GTCTC 5 cut(s) 111, 155, 365, 565, 1596
Alw44I GTGCAC 1 cut(s) 519
AlwI GGATC 2 cut(s) 1543, 1577
Ama87I CYCGRG 1 cut(s) 1480
Aor51HI AGCGCT 1 cut(s) 885
AoxI GGCC 2 cut(s) 165, 705
ApaLI GTGCAC 1 cut(s) 519
ApeKI GCWGC 2 cut(s) 408, 788
ApoI RAATTY 4 cut(s) 133, 475, 661, 1520
AseI ATTAAT 1 cut(s) 1448
AsiGI ACCGGT 1 cut(s) 737
Asp700I GAANNNNTTC 1 cut(s) 717
AspLEI GCGC 3 cut(s) 413, 886, 1084
AspS9I GGNCC 1 cut(s) 309
AsuC2I CCSGG 5 cut(s) 546, 1051, 1216, 1481, 1482
AsuHPI GGTGA 4 cut(s) 727, 755, 1066, 1530
AsuNHI GCTAGC 1 cut(s) 784
AvaI CYCGRG 1 cut(s) 1480
AvaII GGWCC 1 cut(s) 309
BaeGI GKGCMC 1 cut(s) 523
BarI GAAGNNNNNNTAC 2 cut(s) 1139, 1171
BauI CACGAG 1 cut(s) 391
Bbv12I GWGCWC 4 cut(s) 35, 523, 799, 1102
BbvI GCAGC 2 cut(s) 420, 800
BccI CCATC 5 cut(s) 206, 335, 398, 985, 1033
BceAI ACGGC 2 cut(s) 422, 1194
BcgI CGANNNNNNTGC 2 cut(s) 1385, 1419
BciT130I CCWGG 1 cut(s) 941
BciVI GTATCC 1 cut(s) 952
BclI TGATCA 3 cut(s) 252, 669, 814
BcnI CCSGG 5 cut(s) 546, 1051, 1216, 1481, 1482
BcoDI GTCTC 5 cut(s) 111, 155, 365, 565, 1596
BfaI CTAG 3 cut(s) 777, 785, 1599
BfmI CTRYAG 1 cut(s) 1486
BfoI RGCGCY 1 cut(s) 887
BfuI GTATCC 1 cut(s) 952
BisI GCNGC 3 cut(s) 283, 409, 789
BlsI GCNGC 3 cut(s) 284, 410, 790
Bme1390I CCNGG 6 cut(s) 546, 941, 1051, 1216, 1481, 1482
Bme18I GGWCC 1 cut(s) 309
BmeT110I CYCGRG 1 cut(s) 1480
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 2 cut(s) 310, 1362
BmrFI CCNGG 6 cut(s) 546, 941, 1051, 1216, 1481, 1482
BmsI GCATC 3 cut(s) 321, 432, 1129
BmtI GCTAGC 1 cut(s) 788
BpuMI CCSGG 5 cut(s) 546, 1051, 1216, 1481, 1482
Bsa29I ATCGAT 1 cut(s) 201
BsaAI YACGTR 2 cut(s) 238, 524
BsaBI GATNNNNATC 1 cut(s) 1499
BsaJI CCNNGG 6 cut(s) 427, 545, 708, 1000, 1050, 1480
BsaWI WCCGGW 2 cut(s) 737, 757
BsaXI ACNNNNNCTCC 6 cut(s) 33, 63, 838, 868, 1320, 1350
Bse118I RCCGGY 1 cut(s) 737
Bse3DI GCAATG 1 cut(s) 1149
Bse8I GATNNNNATC 1 cut(s) 1499
BseBI CCWGG 1 cut(s) 941
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 6 cut(s) 427, 545, 708, 1000, 1050, 1480
BseGI GGATG 3 cut(s) 295, 423, 559
BseJI GATNNNNATC 1 cut(s) 1499
BseMI GCAATG 1 cut(s) 1149
BseMII CTCAG 3 cut(s) 489, 833, 1219
BseRI GAGGAG 1 cut(s) 859
BseSI GKGCMC 1 cut(s) 523
BseXI GCAGC 2 cut(s) 420, 800
BshFI GGCC 2 cut(s) 167, 707
BshTI ACCGGT 1 cut(s) 737
BshVI ATCGAT 1 cut(s) 201
BsiHKAI GWGCWC 4 cut(s) 35, 523, 799, 1102
BsiHKCI CYCGRG 1 cut(s) 1480
BsiSI CCGG 6 cut(s) 545, 738, 758, 1050, 1215, 1481
BslFI GGGAC 2 cut(s) 892, 1463
BsmAI GTCTC 5 cut(s) 111, 155, 365, 565, 1596
BsmBI CGTCTC 1 cut(s) 155
BsmFI GGGAC 2 cut(s) 892, 1463
BsmI GAATGC 2 cut(s) 62, 392
BsnI GGCC 2 cut(s) 167, 707
BsoBI CYCGRG 1 cut(s) 1480
Bsp1286I GDGCHC 4 cut(s) 35, 523, 799, 1102
Bsp143I GATC 7 cut(s) 252, 276, 669, 732, 814, 1535, 1569
Bsp19I CCATGG 2 cut(s) 427, 1000
BspACI CCGC 2 cut(s) 282, 504
BspANI GGCC 2 cut(s) 167, 707
BspCNI CTCAG 3 cut(s) 490, 834, 1218
BspDI ATCGAT 1 cut(s) 201
BspHI TCATGA 1 cut(s) 249
BspLI GGNNCC 2 cut(s) 310, 1362
BspOI GCTAGC 1 cut(s) 788
BspPI GGATC 2 cut(s) 1543, 1577
BsrDI GCAATG 1 cut(s) 1149
BsrFI RCCGGY 1 cut(s) 737
BssAI RCCGGY 1 cut(s) 737
BssECI CCNNGG 6 cut(s) 427, 545, 708, 1000, 1050, 1480
BssMI GATC 7 cut(s) 252, 276, 669, 732, 814, 1535, 1569
BssSI CACGAG 1 cut(s) 391
BssT1I CCWWGG 3 cut(s) 427, 708, 1000
Bst2BI CACGAG 1 cut(s) 391
Bst2UI CCWGG 1 cut(s) 941
Bst4CI ACNGT 7 cut(s) 458, 531, 772, 863, 1239, 1344, 1632
Bst6I CTCTTC 2 cut(s) 635, 1221
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBAI YACGTR 2 cut(s) 238, 524
BstC8I GCNNGC 3 cut(s) 705, 786, 1189
BstDEI CTNAG 3 cut(s) 498, 842, 1205
BstDSI CCRYGG 2 cut(s) 427, 1000
BstF5I GGATG 3 cut(s) 295, 423, 559
BstH2I RGCGCY 1 cut(s) 887
BstHHI GCGC 3 cut(s) 413, 886, 1084
BstKTI GATC 7 cut(s) 255, 279, 672, 735, 817, 1538, 1572
BstMAI GTCTC 5 cut(s) 111, 155, 365, 565, 1596
BstMBI GATC 7 cut(s) 252, 276, 669, 732, 814, 1535, 1569
BstMWI GCNNNNNNNGC 4 cut(s) 325, 794, 974, 1337
BstNI CCWGG 1 cut(s) 941
BstNSI RCATGY 2 cut(s) 898, 1154
BstSCI CCNGG 6 cut(s) 544, 939, 1049, 1214, 1479, 1480
BstSFI CTRYAG 1 cut(s) 1486
BstSLI GKGCMC 1 cut(s) 523
BstV1I GCAGC 2 cut(s) 420, 800
BstX2I RGATCY 1 cut(s) 1569
BstYI RGATCY 1 cut(s) 1569
Bsu15I ATCGAT 1 cut(s) 201
BsuI GTATCC 1 cut(s) 952
BsuRI GGCC 2 cut(s) 167, 707
BsuTUI ATCGAT 1 cut(s) 201
BtgI CCRYGG 2 cut(s) 427, 1000
BtsCI GGATG 3 cut(s) 295, 423, 559
BtsIMutI CAGTG 5 cut(s) 463, 868, 993, 1244, 1628
Cac8I GCNNGC 3 cut(s) 705, 786, 1189
CciI TCATGA 1 cut(s) 249
CfoI GCGC 3 cut(s) 413, 886, 1084
Cfr10I RCCGGY 1 cut(s) 737
Cfr13I GGNCC 1 cut(s) 309
Cfr9I CCCGGG 1 cut(s) 1480
ClaI ATCGAT 1 cut(s) 201
CspAI ACCGGT 1 cut(s) 737
DdeI CTNAG 3 cut(s) 498, 842, 1205
DpnI GATC 7 cut(s) 254, 278, 671, 734, 816, 1537, 1571
DpnII GATC 7 cut(s) 252, 276, 669, 732, 814, 1535, 1569
DraI TTTAAA 1 cut(s) 1033
EaeI YGGCCR 1 cut(s) 165
Eam1104I CTCTTC 2 cut(s) 635, 1221
EarI CTCTTC 2 cut(s) 635, 1221
Eco130I CCWWGG 3 cut(s) 427, 708, 1000
Eco147I AGGCCT 1 cut(s) 707
Eco47I GGWCC 1 cut(s) 309
Eco47III AGCGCT 1 cut(s) 885
Eco57I CTGAAG 1 cut(s) 1603
Eco88I CYCGRG 1 cut(s) 1480
EcoO109I RGGNCCY 1 cut(s) 309
EcoRI GAATTC 3 cut(s) 475, 661, 1520
EcoRII CCWGG 1 cut(s) 939
EcoT14I CCWWGG 3 cut(s) 427, 708, 1000
ErhI CCWWGG 3 cut(s) 427, 708, 1000
Esp3I CGTCTC 1 cut(s) 155
FalI AAGNNNNNCTT 2 cut(s) 692, 724
FaqI GGGAC 2 cut(s) 892, 1463
FauI CCCGC 1 cut(s) 497
FauNDI CATATG 2 cut(s) 262, 928
FbaI TGATCA 3 cut(s) 252, 669, 814
Fnu4HI GCNGC 3 cut(s) 283, 409, 789
FokI GGATG 3 cut(s) 302, 410, 566
Fsp4HI GCNGC 3 cut(s) 283, 409, 789
FspBI CTAG 3 cut(s) 777, 785, 1599
GlaI GCGC 3 cut(s) 412, 885, 1083
GluI GCNGC 3 cut(s) 283, 409, 789
HaeII RGCGCY 1 cut(s) 887
HaeIII GGCC 2 cut(s) 167, 707
HapII CCGG 6 cut(s) 545, 738, 758, 1050, 1215, 1481
HhaI GCGC 3 cut(s) 413, 886, 1084
Hin6I GCGC 3 cut(s) 411, 884, 1082
HinP1I GCGC 3 cut(s) 411, 884, 1082
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HindIII AAGCTT 1 cut(s) 1410
HinfI GANTC 6 cut(s) 176, 229, 601, 1437, 1576, 1625
HpaII CCGG 6 cut(s) 545, 738, 758, 1050, 1215, 1481
HphI GGTGA 4 cut(s) 727, 755, 1066, 1530
Hpy166II GTNNAC 7 cut(s) 521, 527, 692, 892, 919, 1156, 1387
Hpy188I TCNGA 7 cut(s) 175, 257, 474, 499, 630, 843, 1368
Hpy188III TCNNGA 7 cut(s) 250, 605, 722, 877, 1517, 1573, 1599
Hpy8I GTNNAC 7 cut(s) 521, 527, 692, 892, 919, 1156, 1387
HpyAV CCTTC 2 cut(s) 411, 949
HpyCH4III ACNGT 7 cut(s) 458, 531, 772, 863, 1239, 1344, 1632
HpyCH4IV ACGT 4 cut(s) 237, 246, 523, 1428
HpyCH4V TGCA 8 cut(s) 60, 390, 521, 730, 809, 1142, 1191, 1286
HpyF10VI GCNNNNNNNGC 4 cut(s) 325, 794, 974, 1337
HpyF3I CTNAG 3 cut(s) 498, 842, 1205
HpySE526I ACGT 4 cut(s) 237, 246, 523, 1428
HspAI GCGC 3 cut(s) 411, 884, 1082
Ksp22I TGATCA 3 cut(s) 252, 669, 814
Kzo9I GATC 7 cut(s) 252, 276, 669, 732, 814, 1535, 1569
LmnI GCTCC 5 cut(s) 30, 54, 420, 1328, 1360
Lsp1109I GCAGC 2 cut(s) 420, 800
LweI GCATC 3 cut(s) 321, 432, 1129
MaeI CTAG 3 cut(s) 777, 785, 1599
MaeII ACGT 4 cut(s) 237, 246, 523, 1428
MaeIII GTNAC 1 cut(s) 1069
MalI GATC 7 cut(s) 254, 278, 671, 734, 816, 1537, 1571
MboI GATC 7 cut(s) 252, 276, 669, 732, 814, 1535, 1569
MboII GAAGA 6 cut(s) 151, 643, 649, 652, 964, 1238
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 1 cut(s) 1569
MhlI GDGCHC 4 cut(s) 35, 523, 799, 1102
MluCI AATT 8 cut(s) 133, 355, 475, 661, 810, 1161, 1350, 1520
MlyI GAGTC 1 cut(s) 610
MmeI TCCRAC 1 cut(s) 432
MroXI GAANNNNTTC 1 cut(s) 717
MseI TTAA 8 cut(s) 78, 441, 579, 1032, 1160, 1349, 1422, 1448
MslI CAYNNNNRTG 2 cut(s) 265, 804
MspI CCGG 6 cut(s) 545, 738, 758, 1050, 1215, 1481
MspR9I CCNGG 6 cut(s) 546, 941, 1051, 1216, 1481, 1482
MunI CAATTG 1 cut(s) 810
Mva1269I GAATGC 2 cut(s) 62, 392
MvaI CCWGG 1 cut(s) 941
MwoI GCNNNNNNNGC 4 cut(s) 325, 794, 974, 1337
NciI CCSGG 5 cut(s) 546, 1051, 1216, 1481, 1482
NcoI CCATGG 2 cut(s) 427, 1000
NdeI CATATG 2 cut(s) 262, 928
NdeII GATC 7 cut(s) 252, 276, 669, 732, 814, 1535, 1569
NheI GCTAGC 1 cut(s) 784
NlaIV GGNNCC 2 cut(s) 310, 1362
NmeAIII GCCGAG 1 cut(s) 143
NmuCI GTSAC 1 cut(s) 1069
NspI RCATGY 2 cut(s) 898, 1154
PagI TCATGA 1 cut(s) 249
PceI AGGCCT 1 cut(s) 707
PciI ACATGT 1 cut(s) 894
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 2 cut(s) 62, 392
PdmI GAANNNNTTC 1 cut(s) 717
PfeI GAWTC 5 cut(s) 176, 229, 1437, 1576, 1625
PfoI TCCNGGA 1 cut(s) 939
PinAI ACCGGT 1 cut(s) 737
PkrI GCNGC 3 cut(s) 284, 410, 790
PleI GAGTC 1 cut(s) 609
PpsI GAGTC 1 cut(s) 609
Ppu21I YACGTR 2 cut(s) 238, 524
PpuMI RGGWCCY 1 cut(s) 309
PscI ACATGT 1 cut(s) 894
PshBI ATTAAT 1 cut(s) 1448
Psp5II RGGWCCY 1 cut(s) 309
Psp6I CCWGG 1 cut(s) 939
PspGI CCWGG 1 cut(s) 939
PspN4I GGNNCC 2 cut(s) 310, 1362
PspPI GGNCC 1 cut(s) 309
PspPPI RGGWCCY 1 cut(s) 309
PsuI RGATCY 1 cut(s) 1569
RseI CAYNNNNRTG 2 cut(s) 265, 804
SaqAI TTAA 8 cut(s) 78, 441, 579, 1032, 1160, 1349, 1422, 1448
SatI GCNGC 3 cut(s) 283, 409, 789
Sau3AI GATC 7 cut(s) 252, 276, 669, 732, 814, 1535, 1569
Sau96I GGNCC 1 cut(s) 309
SchI GAGTC 1 cut(s) 610
ScrFI CCNGG 6 cut(s) 546, 941, 1051, 1216, 1481, 1482
SduI GDGCHC 4 cut(s) 35, 523, 799, 1102
SfaNI GCATC 3 cut(s) 321, 432, 1129
SfcI CTRYAG 1 cut(s) 1486
SgrAI CRCCGGYG 1 cut(s) 737
SinI GGWCC 1 cut(s) 309
SmaI CCCGGG 1 cut(s) 1482
SmiMI CAYNNNNRTG 2 cut(s) 265, 804
Sse9I AATT 8 cut(s) 133, 355, 475, 661, 810, 1161, 1350, 1520
SseBI AGGCCT 1 cut(s) 707
SsiI CCGC 2 cut(s) 282, 504
SspMI CTAG 3 cut(s) 777, 785, 1599
StuI AGGCCT 1 cut(s) 707
StyD4I CCNGG 6 cut(s) 544, 939, 1049, 1214, 1479, 1480
StyI CCWWGG 3 cut(s) 427, 708, 1000
TaaI ACNGT 7 cut(s) 458, 531, 772, 863, 1239, 1344, 1632
TaiI ACGT 4 cut(s) 240, 249, 526, 1431
TaqI TCGA 4 cut(s) 201, 211, 572, 1548
TasI AATT 8 cut(s) 133, 355, 475, 661, 810, 1161, 1350, 1520
TauI GCSGC 1 cut(s) 285
TfiI GAWTC 5 cut(s) 176, 229, 1437, 1576, 1625
Tru1I TTAA 8 cut(s) 78, 441, 579, 1032, 1160, 1349, 1422, 1448
Tru9I TTAA 8 cut(s) 78, 441, 579, 1032, 1160, 1349, 1422, 1448
TscAI CASTG 5 cut(s) 463, 868, 993, 1244, 1635
TseFI GTSAC 1 cut(s) 1069
TseI GCWGC 2 cut(s) 408, 788
Tsp45I GTSAC 1 cut(s) 1069
TspDTI ATGAA 8 cut(s) 69, 306, 552, 650, 821, 1160, 1274, 1466
TspGWI ACGGA 3 cut(s) 360, 655, 1516
TspMI CCCGGG 1 cut(s) 1480
TspRI CASTG 5 cut(s) 463, 868, 993, 1244, 1635
VneI GTGCAC 1 cut(s) 519
VpaK11BI GGWCC 1 cut(s) 309
VspI ATTAAT 1 cut(s) 1448
XapI RAATTY 4 cut(s) 133, 475, 661, 1520
XbaI TCTAGA 1 cut(s) 1598
XceI RCATGY 2 cut(s) 898, 1154
XmaI CCCGGG 1 cut(s) 1480
XmnI GAANNNNTTC 1 cut(s) 717
XspI CTAG 3 cut(s) 777, 785, 1599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.