FvH4_7g28451

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0011997
Physical Location & Seq
Forward (+)
21082616 .. 21084218
1603 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g28451.t1

Sequence Viewer

Length: 1536 bp
ATGTCAAGTACTCTCAGAGCCACACTCTCACATTTTCTGGAACAATGCAAGAACATGAGAGAGCTCAAACAAATCCACACCCACATCATAAAGTCCCCATTTTTACTCGAACAAGACCAATCCTTTCTCAACACCCGTCTCCTCTTCTTCTGCGCTCTATCCGATTCGGGTTCCCAAACCTACGCCGCCGGAGTTTTCCGGGTCATAAAGAACCCGAATCTTTATCTCTACAACATCATGATCAGAGCCTACACGTCTATAAAGGCCAATGCTTTTGATGAACCGGCGTCGTTTCGGTCGTTGGAGTTGTATAAGCAAATGGTGGGTGATGGGATTAGGCCCGATTGCCTCACGTTCCCGTTTCTTGTGAAGGAATGCGCGGCGAGGATCGACGGCGGGAATGGCCGGAGCGTTCATGGGCAGGTGGTTAAGTATGGGTTCAGAAAGGATTTGTTTGTTCAAAACTCGTTGATGAATATGTACTCTGTTTTCGGGTCTTTGATTTGTGCACGGAAGGTGTTCGATGAAATAACTGAGAGAGATGTGGTGTCGTGGAACTCGATGATTAAGGGGTATTTGAGAAGTGGGGATCTTGATGAGGCGATTGAGGTGTTTAGGGAGATGAAGAAGAGGAGTGTTTTTAGTTGGAATTCGATTATAACCGGGTGTGTTCAAGGCGGTCGGGCGAGGGAGGCATTGGATTTTTTTCATGAAATGCAGGTTGTTGAGGGTGTTAGGCCGGATAAGATTACGATTGCTAGTGTTCTTGCGGCTTGTGCTCATCTTGGCGAGGTTGATAATGGGACATGGGTGCATGGGTACTTGAGGAGAAGTGGGGTGGAGAGTGATGTGGTTATTGGTACAGCTCTGGTTGATATGTATGGTAAATGTGGGTGTGTGGAGAAAGCTTATGAGGTGTTTATGGAAATGCCGAAGAAAGATACTTTGGCGTGGACTGCTATGATTTCAGTTTTTGCTCTCCATGGGTTTGGCAATGAGGCGTTTGATCTTTTTGAAAAGATGGAAGCAGCTGGGGTTGAGCCGAATCATGTGACGTTTGTTGGGTTATTGTCAGCTTGTGCTCATTCTGGTTTAGTAGAGAAAGGTCGATGGTGTTTTGATGTGATGAGACGTGTTTATTTGATTGAACCGCAACTTTATCATTATGCATGCATGGTTGATATACTTAGCAGAGCCGGGCTAATTGAAGAGGCGGAGAGGTTGATAAGAAGCATGCCAATGAAGCCAGATGTATATGTATGGGGTGCATTGCTTGGAGGTTGTCAAATACATGGACAGGTTGAGATAGGAGAAATGGCGGCCCATCGTTTAATTGATATGGAACCTCTGAACCATGCTTTTTATGTCAACTTGTGCGATATCTATGCCAAAACTGGTAGGTTTAATGATGTGAAAACAGTTAAGTCACTAATGAAAGAAAGAGGGATAAAAAAGGAAGTTGCAGGTTGTAGCATGATTGAAGTCGATGGAGTTGTACTTGAATTCTCAGTGAGAGGATCCCTGATATTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

512

Amino Acids

57.52

Weight (kDa)

6.55

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 180 - 213 3.4e-11 PPR repeat family
PPR_1 PF12854 180 - 209 6.5e-09 PPR repeat
PPR PF01535 183 - 212 4.4e-11 PPR repeat
PPR_2 PF13041 212 - 261 5.8e-09 PPR repeat family
PPR PF01535 214 - 240 2.5e-06 PPR repeat
PPR_2 PF13041 314 - 362 4.3e-10 PPR repeat family
PPR PF01535 317 - 346 6.9e-06 PPR repeat
E_motif PF20431 431 - 494 1.5e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14170 AT4G14170
fragaria_vesca FvH4_7g28451
malus_domestica MD01G1188300.v1.1
prunus_persica Prupe.2G284600_v2.0.a1
pyrus_communis pycom01g20010
rosa_chinensis RchiOBHm_Chr1g0375631 RchiOBHm_Chr1g0375671
rosa_laevigata RLG00000026661
rosa_multiflora Rmu_co8281021.1_g000001 Rmu_co8425983.1_g000001 Rmu_sc0001644.1_g000022
rosa_roxburghii Rroxscaffold_4G00282680
rosa_rugosa Rorug01G0391700
rosa_samantha Rh1AG402600 Rh1BG366800 Rh1CG379500
rosa_wichuraiana Rw1G035750 Rw1G035770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 659
AarI CACCTGC 1 cut(s) 412
Acc36I ACCTGC 3 cut(s) 412, 709, 1454
AccII CGCG 1 cut(s) 380
AciI CCGC 8 cut(s) 186, 380, 396, 678, 770, 1151, 1214, 1319
AclWI GGATC 4 cut(s) 395, 597, 1512, 1525
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 2 cut(s) 649, 1502
AcyI GRCGYC 1 cut(s) 287
AfaI GTAC 5 cut(s) 10, 482, 821, 862, 1497
AflIII ACRYGT 2 cut(s) 252, 1132
AgsI TTSAA 7 cut(s) 461, 674, 1016, 1148, 1208, 1481, 1502
AjiI CACGTC 2 cut(s) 255, 1133
AjuI GAANNNNNNNTTGG 2 cut(s) 929, 961
AluBI AGCT 5 cut(s) 64, 866, 908, 1031, 1076
AluI AGCT 5 cut(s) 64, 866, 908, 1031, 1076
Alw21I GWGCWC 4 cut(s) 66, 511, 781, 1084
Alw26I GTCTC 2 cut(s) 143, 1123
Alw44I GTGCAC 1 cut(s) 507
AlwI GGATC 4 cut(s) 395, 597, 1512, 1525
AoxI GGCC 5 cut(s) 264, 338, 403, 737, 1320
ApaLI GTGCAC 1 cut(s) 507
ApeKI GCWGC 1 cut(s) 1028
ApoI RAATTY 2 cut(s) 649, 1502
Asp700I GAANNNNTTC 1 cut(s) 518
AspLEI GCGC 2 cut(s) 155, 380
AspS9I GGNCC 2 cut(s) 339, 1321
AsuC2I CCSGG 3 cut(s) 200, 664, 1198
AsuHPI GGTGA 1 cut(s) 338
BaeGI GKGCMC 1 cut(s) 511
BamHI GGATCC 1 cut(s) 1517
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 4 cut(s) 66, 511, 781, 1084
BbvI GCAGC 1 cut(s) 1040
BccI CCATC 5 cut(s) 323, 1015, 1104, 1332, 1481
BceAI ACGGC 1 cut(s) 409
BcgI CGANNNNNNTGC 2 cut(s) 1367, 1401
BclI TGATCA 1 cut(s) 240
BcnI CCSGG 3 cut(s) 200, 664, 1198
BcoDI GTCTC 2 cut(s) 143, 1123
BfaI CTAG 1 cut(s) 759
BfuAI ACCTGC 3 cut(s) 412, 709, 1454
BisI GCNGC 5 cut(s) 186, 381, 771, 1029, 1320
BlsI GCNGC 5 cut(s) 187, 382, 772, 1030, 1321
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 3 cut(s) 200, 664, 1198
BmgBI CACGTC 2 cut(s) 255, 1133
BmgT120I GGNCC 2 cut(s) 339, 1321
BmiI GGNNCC 3 cut(s) 172, 1344, 1519
BmrFI CCNGG 3 cut(s) 200, 664, 1198
BplI GAGNNNNNCTC 2 cut(s) 9, 41
BpuEI CTTGAG 1 cut(s) 844
BpuMI CCSGG 3 cut(s) 200, 664, 1198
BsaHI GRCGYC 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 982
Bse118I RCCGGY 1 cut(s) 283
Bse1I ACTGG 1 cut(s) 1399
Bse3DI GCAATG 2 cut(s) 1000, 1268
BseDI CCNNGG 1 cut(s) 982
BseMI GCAATG 2 cut(s) 1000, 1268
BseMII CTCAG 3 cut(s) 28, 525, 1521
BseNI ACTGG 1 cut(s) 1399
BseRI GAGGAG 3 cut(s) 131, 646, 841
BseSI GKGCMC 1 cut(s) 511
BseXI GCAGC 1 cut(s) 1040
BseYI CCCAGC 1 cut(s) 1031
Bsh1236I CGCG 1 cut(s) 380
Bsh1285I CGRYCG 2 cut(s) 299, 682
BshFI GGCC 5 cut(s) 266, 340, 405, 739, 1322
BsiEI CGRYCG 2 cut(s) 299, 682
BsiHKAI GWGCWC 4 cut(s) 66, 511, 781, 1084
BsiSI CCGG 7 cut(s) 189, 199, 284, 406, 663, 740, 1197
BslFI GGGAC 2 cut(s) 79, 817
BsmAI GTCTC 2 cut(s) 143, 1123
BsmBI CGTCTC 2 cut(s) 143, 1123
BsmFI GGGAC 2 cut(s) 79, 817
BsmI GAATGC 1 cut(s) 380
BsnI GGCC 5 cut(s) 266, 340, 405, 739, 1322
Bsp1286I GDGCHC 4 cut(s) 66, 511, 781, 1084
Bsp143I GATC 5 cut(s) 240, 387, 589, 1006, 1517
Bsp19I CCATGG 1 cut(s) 982
BspACI CCGC 8 cut(s) 186, 380, 396, 678, 770, 1151, 1214, 1319
BspANI GGCC 5 cut(s) 266, 340, 405, 739, 1322
BspCNI CTCAG 3 cut(s) 27, 526, 1520
BspFNI CGCG 1 cut(s) 380
BspHI TCATGA 2 cut(s) 237, 709
BspLI GGNNCC 3 cut(s) 172, 1344, 1519
BspMI ACCTGC 3 cut(s) 412, 709, 1454
BspPI GGATC 4 cut(s) 395, 597, 1512, 1525
BsrDI GCAATG 2 cut(s) 1000, 1268
BsrFI RCCGGY 1 cut(s) 283
BsrI ACTGG 1 cut(s) 1399
BssAI RCCGGY 1 cut(s) 283
BssECI CCNNGG 1 cut(s) 982
BssMI GATC 5 cut(s) 240, 387, 589, 1006, 1517
BssNI GRCGYC 1 cut(s) 287
BssT1I CCWWGG 1 cut(s) 982
Bst4CI ACNGT 1 cut(s) 1420
Bst6I CTCTTC 3 cut(s) 149, 623, 1203
BstACI GRCGYC 1 cut(s) 287
BstC8I GCNNGC 2 cut(s) 1171, 1235
BstDEI CTNAG 4 cut(s) 14, 534, 1187, 1507
BstDSI CCRYGG 1 cut(s) 982
BstFNI CGCG 1 cut(s) 380
BstHHI GCGC 2 cut(s) 155, 380
BstKTI GATC 5 cut(s) 243, 390, 592, 1009, 1520
BstMAI GTCTC 2 cut(s) 143, 1123
BstMBI GATC 5 cut(s) 240, 387, 589, 1006, 1517
BstMCI CGRYCG 2 cut(s) 299, 682
BstMWI GCNNNNNNNGC 5 cut(s) 402, 692, 776, 956, 1243
BstNSI RCATGY 2 cut(s) 1173, 1237
BstSCI CCNGG 3 cut(s) 198, 662, 1196
BstSLI GKGCMC 1 cut(s) 511
BstUI CGCG 1 cut(s) 380
BstV1I GCAGC 1 cut(s) 1040
BstX2I RGATCY 2 cut(s) 589, 1517
BstXI CCANNNNNNTGG 1 cut(s) 989
BstYI RGATCY 2 cut(s) 589, 1517
BsuRI GGCC 5 cut(s) 266, 340, 405, 739, 1322
BtgI CCRYGG 1 cut(s) 982
BtrI CACGTC 2 cut(s) 255, 1133
BtsIMutI CAGTG 1 cut(s) 1515
BveI ACCTGC 3 cut(s) 412, 709, 1454
Cac8I GCNNGC 2 cut(s) 1171, 1235
CciI TCATGA 2 cut(s) 237, 709
CfoI GCGC 2 cut(s) 155, 380
Cfr10I RCCGGY 1 cut(s) 283
Cfr13I GGNCC 2 cut(s) 339, 1321
CseI GACGC 1 cut(s) 276
Csp6I GTAC 5 cut(s) 9, 481, 820, 861, 1496
CviQI GTAC 5 cut(s) 9, 481, 820, 861, 1496
DdeI CTNAG 4 cut(s) 14, 534, 1187, 1507
DpnI GATC 5 cut(s) 242, 389, 591, 1008, 1519
DpnII GATC 5 cut(s) 240, 387, 589, 1006, 1517
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 3 cut(s) 149, 623, 1203
EarI CTCTTC 3 cut(s) 149, 623, 1203
EciI GGCGGA 1 cut(s) 1229
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 982
Eco24I GRGCYC 1 cut(s) 66
Eco32I GATATC 1 cut(s) 1381
Eco53kI GAGCTC 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 64
EcoRI GAATTC 2 cut(s) 649, 1502
EcoRV GATATC 1 cut(s) 1381
EcoT14I CCWWGG 1 cut(s) 982
EcoT22I ATGCAT 2 cut(s) 1171, 1175
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 982
Esp3I CGTCTC 2 cut(s) 143, 1123
FaqI GGGAC 2 cut(s) 79, 817
FauI CCCGC 1 cut(s) 389
FbaI TGATCA 1 cut(s) 240
Fnu4HI GCNGC 5 cut(s) 186, 381, 771, 1029, 1320
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 5 cut(s) 186, 381, 771, 1029, 1320
FspBI CTAG 1 cut(s) 759
GlaI GCGC 2 cut(s) 154, 379
GluI GCNGC 5 cut(s) 186, 381, 771, 1029, 1320
GsaI CCCAGC 1 cut(s) 1035
HaeIII GGCC 5 cut(s) 266, 340, 405, 739, 1322
HapII CCGG 7 cut(s) 189, 199, 284, 406, 663, 740, 1197
HgaI GACGC 1 cut(s) 276
HhaI GCGC 2 cut(s) 155, 380
Hin1I GRCGYC 1 cut(s) 287
Hin6I GCGC 2 cut(s) 153, 378
HinP1I GCGC 2 cut(s) 153, 378
HincII GTYRAC 1 cut(s) 1369
HindII GTYRAC 1 cut(s) 1369
HindIII AAGCTT 1 cut(s) 906
HinfI GANTC 3 cut(s) 164, 217, 1045
HpaII CCGG 7 cut(s) 189, 199, 284, 406, 663, 740, 1197
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 3 cut(s) 509, 954, 1369
Hpy188I TCNGA 5 cut(s) 17, 163, 245, 443, 1350
Hpy188III TCNNGA 4 cut(s) 38, 238, 593, 710
Hpy8I GTNNAC 3 cut(s) 509, 954, 1369
Hpy99I CGWCG 2 cut(s) 292, 395
HpyAV CCTTC 2 cut(s) 364, 508
HpyCH4III ACNGT 1 cut(s) 1420
HpyCH4IV ACGT 4 cut(s) 254, 353, 1055, 1132
HpyCH4V TGCA 8 cut(s) 48, 509, 718, 814, 1169, 1173, 1268, 1463
HpyF10VI GCNNNNNNNGC 5 cut(s) 402, 692, 776, 956, 1243
HpyF3I CTNAG 4 cut(s) 14, 534, 1187, 1507
HpySE526I ACGT 4 cut(s) 254, 353, 1055, 1132
Hsp92I GRCGYC 1 cut(s) 287
HspAI GCGC 2 cut(s) 153, 378
Ksp22I TGATCA 1 cut(s) 240
Kzo9I GATC 5 cut(s) 240, 387, 589, 1006, 1517
LmnI GCTCC 1 cut(s) 408
Lsp1109I GCAGC 1 cut(s) 1040
MaeI CTAG 1 cut(s) 759
MaeII ACGT 4 cut(s) 254, 353, 1055, 1132
MaeIII GTNAC 2 cut(s) 1051, 1425
MalI GATC 5 cut(s) 242, 389, 591, 1008, 1519
MboI GATC 5 cut(s) 240, 387, 589, 1006, 1517
MboII GAAGA 6 cut(s) 136, 139, 637, 640, 946, 1220
MflI RGATCY 2 cut(s) 589, 1517
MhlI GDGCHC 4 cut(s) 66, 511, 781, 1084
MluCI AATT 4 cut(s) 649, 1203, 1332, 1502
MmeI TCCRAC 2 cut(s) 282, 626
Mph1103I ATGCAT 2 cut(s) 1171, 1175
MroXI GAANNNNTTC 1 cut(s) 518
MseI TTAA 5 cut(s) 429, 567, 1331, 1404, 1422
MslI CAYNNNNRTG 1 cut(s) 1238
MspA1I CMGCKG 1 cut(s) 1031
MspI CCGG 7 cut(s) 189, 199, 284, 406, 663, 740, 1197
MspR9I CCNGG 3 cut(s) 200, 664, 1198
Mva1269I GAATGC 1 cut(s) 380
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 5 cut(s) 402, 692, 776, 956, 1243
NciI CCSGG 3 cut(s) 200, 664, 1198
NcoI CCATGG 1 cut(s) 982
NdeII GATC 5 cut(s) 240, 387, 589, 1006, 1517
NlaIV GGNNCC 3 cut(s) 172, 1344, 1519
NmuCI GTSAC 2 cut(s) 1051, 1425
NsiI ATGCAT 2 cut(s) 1171, 1175
NspI RCATGY 2 cut(s) 1173, 1237
PaeI GCATGC 2 cut(s) 1173, 1237
PagI TCATGA 2 cut(s) 237, 709
PaqCI CACCTGC 1 cut(s) 412
PcsI WCGNNNNNNNCGW 2 cut(s) 296, 557
PctI GAATGC 1 cut(s) 380
PdmI GAANNNNTTC 1 cut(s) 518
PfeI GAWTC 3 cut(s) 164, 217, 1045
PkrI GCNGC 5 cut(s) 187, 382, 772, 1030, 1321
PsiI TTATAA 1 cut(s) 659
Psp124BI GAGCTC 1 cut(s) 66
PspFI CCCAGC 1 cut(s) 1031
PspN4I GGNNCC 3 cut(s) 172, 1344, 1519
PspPI GGNCC 2 cut(s) 339, 1321
PsuI RGATCY 2 cut(s) 589, 1517
PvuII CAGCTG 1 cut(s) 1031
RsaI GTAC 5 cut(s) 10, 482, 821, 862, 1497
RsaNI GTAC 5 cut(s) 9, 481, 820, 861, 1496
RseI CAYNNNNRTG 1 cut(s) 1238
SacI GAGCTC 1 cut(s) 66
SaqAI TTAA 5 cut(s) 429, 567, 1331, 1404, 1422
SatI GCNGC 5 cut(s) 186, 381, 771, 1029, 1320
Sau3AI GATC 5 cut(s) 240, 387, 589, 1006, 1517
Sau96I GGNCC 2 cut(s) 339, 1321
ScaI AGTACT 1 cut(s) 10
ScrFI CCNGG 3 cut(s) 200, 664, 1198
SduI GDGCHC 4 cut(s) 66, 511, 781, 1084
SmiMI CAYNNNNRTG 1 cut(s) 1238
SmlI CTYRAG 1 cut(s) 823
SmoI CTYRAG 1 cut(s) 823
SphI GCATGC 2 cut(s) 1173, 1237
Sse9I AATT 4 cut(s) 649, 1203, 1332, 1502
SsiI CCGC 8 cut(s) 186, 380, 396, 678, 770, 1151, 1214, 1319
SspMI CTAG 1 cut(s) 759
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 3 cut(s) 198, 662, 1196
StyI CCWWGG 1 cut(s) 982
TaaI ACNGT 1 cut(s) 1420
TaiI ACGT 4 cut(s) 257, 356, 1058, 1135
TaqI TCGA 7 cut(s) 108, 390, 522, 560, 653, 1108, 1485
TaqII GACCGA 1 cut(s) 285
TasI AATT 4 cut(s) 649, 1203, 1332, 1502
TatI WGTACW 3 cut(s) 8, 480, 1495
TauI GCSGC 4 cut(s) 188, 383, 773, 1322
TfiI GAWTC 3 cut(s) 164, 217, 1045
Tru1I TTAA 5 cut(s) 429, 567, 1331, 1404, 1422
Tru9I TTAA 5 cut(s) 429, 567, 1331, 1404, 1422
TscAI CASTG 1 cut(s) 1515
TseFI GTSAC 2 cut(s) 1051, 1425
TseI GCWGC 1 cut(s) 1028
Tsp45I GTSAC 2 cut(s) 1051, 1425
TspDTI ATGAA 9 cut(s) 294, 404, 488, 540, 638, 698, 726, 1256, 1448
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 1 cut(s) 1515
VneI GTGCAC 1 cut(s) 507
XapI RAATTY 2 cut(s) 649, 1502
XceI RCATGY 2 cut(s) 1173, 1237
XmnI GAANNNNTTC 1 cut(s) 518
XspI CTAG 1 cut(s) 759
ZrmI AGTACT 1 cut(s) 10
Zsp2I ATGCAT 2 cut(s) 1171, 1175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.