RLG00000026661

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0011997
Physical Location & Seq
Reverse (-)
4689978 .. 4691504
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026661

Sequence Viewer

Length: 1527 bp
ATGAGAGAGCTCAAACAAATCCACACCCACATCATAAAGTCCCCATTTTTACTCAAACAAGACCAATCCTCTCTCATCACTCGTCTCCTATTCTTCTGTGCTCTATCCGATTCGGGTTCTCAGACCTACGCCACCGCAGTTTTCCGGGTCATAAAGAACCCGACTCTCTACGTCTACAACATCATGATCAGAGCATACGCGTGTGAAAAGACCAGTGCTTTGGATAAACCTGCTTCGTTTCGGTCGTTGGTGTTGTATAAGCAAATGGTTGGTAATGGGATTGGGCCCAGTTGGCTCACTTTTCCGTTTCTTGTGAAGGAATGCGCGGCGAGGAGTGAGGGCGGCGCTGGCCGGAGCGTTCATGGCCAAGTGGTTAAGTACGGGTGTGAAGATGATGTGTTTGTTCGAAACTCGTTGATGAACATGTACTCTGTTTGTGGGTCTTTGAGTTGCGCACGCCAGGTGTTTGATGAAATGCCTGAGAGAGATGTGGTTTCGTGGAACTCGATGATAAAGGGGTGTTTGAGAAGCGGGGAACTTGATGAGGCGATGGGTGTGTTTGGGAAGATGAAGCAGAGGAGTATTTTTAGTTGGAATTCCATTATAACCGGGTGTGTTCAAGGCGGTCGGCCGAAGGAGGCATTGGAACTTTTTCATGAAATGCAGGTTGCAGGCGGTGTTAGACCGGATAAGATTACGATTGCTAGTGTCCTTGCGGCTTGTGCTCATCTTGGCGCGGTTGATCACGGGACATGGGTGCATGGGTACCTGAGGAGAAGTGGGCTGGAGAGTGATGTGGTTATTGGAACAGCTCTGGTTGACATGTATGGTAAATGTGGATGTGTGGAAAAAGCTTATGAGGTCTTTAAGGAAATGCCGAAGAAAGATACTTTGGCATGGACAGCTATGATTTCGGTTTTTGCTCTCCATGGGTTTGGCAGTGAGGCTTTTGATCTTTTTGAAAAGATGGAAGCAACTGGGGTTGAGCCGAATCATGTGACTTTTGTTGGGTTGTTGTCAGCTTGTGCTCATTCTGGTTTAGTAGGGAAAGGTCGCTGGTGTTTTGATGTGATGAGACGTGTTTACTTAATTGAACCACAGCTTTATCATTATGCATGCATGGTTGATATACTTAGCAGAGCCGGGCTAATTGAAGAGGCGGAGAGGTTGATAAGAAGCATGCCAATGAAGCCAGATGTATTTGTTTGGGGTGCATTACTTGGAGGTTGTCAAATACATGGAAAGGTTGAGATAGGAGAACTGGTGGCCCGTCATTTAATTGATATGGAACCTCTGAACCATGCTTTTTATGTCAACTTGTGCGATATATATGCCAAAGCTGGTAGATTTAATGATGTGAAAACAATTAAATCACTAATGAAAGAAAGAGGGATCAAAAAGGAAGTTGCAGGCTGTAGCCTGATTGAAGTCGATGGAGTTGTTCTTGAATTCTCAGTCAGAGGATCCCCTGATGTTGCAATGGATGAAGTATTATGGGTCTTGAGTCAGTTGAATAATGAGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

509

Amino Acids

56.33

Weight (kDa)

6.91

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 162 - 195 1.8e-08 PPR repeat family
PPR_1 PF12854 162 - 191 6e-06 PPR repeat
PPR PF01535 165 - 194 3.9e-09 PPR repeat
PPR_2 PF13041 194 - 243 1.5e-09 PPR repeat family
PPR PF01535 196 - 225 3.1e-08 PPR repeat
PPR_2 PF13041 296 - 344 1.3e-09 PPR repeat family
E_motif PF20431 413 - 476 6.7e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14170 AT4G14170
fragaria_vesca FvH4_7g28451
malus_domestica MD01G1188300.v1.1
prunus_persica Prupe.2G284600_v2.0.a1
pyrus_communis pycom01g20010
rosa_chinensis RchiOBHm_Chr1g0375631 RchiOBHm_Chr1g0375671
rosa_laevigata RLG00000026661
rosa_multiflora Rmu_co8281021.1_g000001 Rmu_co8425983.1_g000001 Rmu_sc0001644.1_g000022
rosa_roxburghii Rroxscaffold_4G00282680
rosa_rugosa Rorug01G0391700
rosa_samantha Rh1AG402600 Rh1BG366800 Rh1CG379500
rosa_wichuraiana Rw1G035750 Rw1G035770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 605
Acc16I TGCGCA 1 cut(s) 454
Acc36I ACCTGC 2 cut(s) 238, 655
Acc65I GGTACC 1 cut(s) 765
AccB1I GGYRCC 1 cut(s) 765
AccI GTMKAC 1 cut(s) 174
AccII CGCG 3 cut(s) 200, 326, 737
AciI CCGC 9 cut(s) 135, 326, 342, 531, 624, 675, 716, 737, 1160
AclWI GGATC 3 cut(s) 1400, 1458, 1471
AcoI YGGCCR 3 cut(s) 349, 364, 629
AcsI RAATTY 2 cut(s) 595, 1448
AfaI GTAC 3 cut(s) 380, 428, 767
AflIII ACRYGT 4 cut(s) 198, 423, 822, 1078
AgsI TTSAA 7 cut(s) 620, 962, 1094, 1154, 1427, 1448, 1513
AjiI CACGTC 1 cut(s) 1079
AjnI CCWGG 1 cut(s) 459
AjuI GAANNNNNNNTTGG 4 cut(s) 626, 658, 875, 907
AluBI AGCT 7 cut(s) 10, 812, 854, 905, 1022, 1102, 1340
AluI AGCT 7 cut(s) 10, 812, 854, 905, 1022, 1102, 1340
Alw21I GWGCWC 4 cut(s) 12, 103, 727, 1030
Alw26I GTCTC 2 cut(s) 89, 1069
AlwI GGATC 3 cut(s) 1400, 1458, 1471
AoxI GGCC 6 cut(s) 284, 349, 364, 629, 1266, 1521
ApaI GGGCCC 1 cut(s) 288
ApoI RAATTY 2 cut(s) 595, 1448
ArsI GACNNNNNNTTYG 2 cut(s) 202, 234
Asp700I GAANNNNTTC 1 cut(s) 651
Asp718I GGTACC 1 cut(s) 765
AspLEI GCGC 4 cut(s) 326, 347, 455, 737
AspS9I GGNCC 3 cut(s) 284, 285, 1267
AsuC2I CCSGG 3 cut(s) 146, 610, 1144
AsuII TTCGAA 1 cut(s) 406
AxyI CCTNAGG 1 cut(s) 770
BaeGI GKGCMC 1 cut(s) 288
BalI TGGCCA 1 cut(s) 366
BamHI GGATCC 1 cut(s) 1463
BanI GGYRCC 1 cut(s) 765
BanII GRGCYC 2 cut(s) 12, 288
Bbv12I GWGCWC 4 cut(s) 12, 103, 727, 1030
BccI CCATC 3 cut(s) 544, 961, 1427
BcgI CGANNNNNNTGC 2 cut(s) 1313, 1347
BciT130I CCWGG 1 cut(s) 461
BclI TGATCA 2 cut(s) 186, 742
BcnI CCSGG 3 cut(s) 146, 610, 1144
BcoDI GTCTC 2 cut(s) 89, 1069
BfaI CTAG 2 cut(s) 705, 1525
BfmI CTRYAG 1 cut(s) 1414
BfoI RGCGCY 1 cut(s) 348
BfuAI ACCTGC 2 cut(s) 238, 655
BglI GCCNNNNNGGC 1 cut(s) 292
BisI GCNGC 3 cut(s) 327, 343, 717
BlsI GCNGC 3 cut(s) 328, 344, 718
Bme1390I CCNGG 4 cut(s) 146, 461, 610, 1144
BmgBI CACGTC 1 cut(s) 1079
BmgT120I GGNCC 3 cut(s) 284, 285, 1267
BmiI GGNNCC 4 cut(s) 286, 767, 1290, 1465
BmrFI CCNGG 4 cut(s) 146, 461, 610, 1144
BmrI ACTGGG 2 cut(s) 282, 987
BmuI ACTGGG 2 cut(s) 282, 987
BpmI CTGGAG 1 cut(s) 806
Bpu14I TTCGAA 1 cut(s) 406
BpuEI CTTGAG 1 cut(s) 1522
BpuMI CCSGG 3 cut(s) 146, 610, 1144
BsaJI CCNNGG 1 cut(s) 928
BsaWI WCCGGW 1 cut(s) 685
BsaXI ACNNNNNCTCC 2 cut(s) 1248, 1278
Bse1I ACTGG 4 cut(s) 213, 288, 982, 1266
Bse21I CCTNAGG 1 cut(s) 770
Bse3DI GCAATG 1 cut(s) 1485
BseBI CCWGG 1 cut(s) 461
BseDI CCNNGG 1 cut(s) 928
BseGI GGATG 2 cut(s) 845, 1489
BseMI GCAATG 1 cut(s) 1485
BseMII CTCAG 4 cut(s) 134, 471, 761, 1467
BseNI ACTGG 4 cut(s) 213, 288, 982, 1266
BseRI GAGGAG 3 cut(s) 346, 592, 787
BseSI GKGCMC 1 cut(s) 288
BseX3I CGGCCG 1 cut(s) 629
Bsh1236I CGCG 3 cut(s) 200, 326, 737
Bsh1285I CGRYCG 3 cut(s) 245, 628, 632
BshFI GGCC 6 cut(s) 286, 351, 366, 631, 1268, 1523
BshNI GGYRCC 1 cut(s) 765
BsiEI CGRYCG 3 cut(s) 245, 628, 632
BsiHKAI GWGCWC 4 cut(s) 12, 103, 727, 1030
BsiSI CCGG 5 cut(s) 145, 352, 609, 686, 1143
BslFI GGGAC 2 cut(s) 25, 763
BsmAI GTCTC 2 cut(s) 89, 1069
BsmBI CGTCTC 2 cut(s) 89, 1069
BsmFI GGGAC 2 cut(s) 25, 763
BsmI GAATGC 1 cut(s) 326
BsnI GGCC 6 cut(s) 286, 351, 366, 631, 1268, 1523
Bsp119I TTCGAA 1 cut(s) 406
Bsp120I GGGCCC 1 cut(s) 284
Bsp1286I GDGCHC 5 cut(s) 12, 103, 288, 727, 1030
Bsp143I GATC 5 cut(s) 186, 742, 952, 1392, 1463
Bsp19I CCATGG 1 cut(s) 928
BspACI CCGC 9 cut(s) 135, 326, 342, 531, 624, 675, 716, 737, 1160
BspANI GGCC 6 cut(s) 286, 351, 366, 631, 1268, 1523
BspCNI CTCAG 4 cut(s) 133, 472, 762, 1466
BspFNI CGCG 3 cut(s) 200, 326, 737
BspHI TCATGA 2 cut(s) 183, 655
BspLI GGNNCC 4 cut(s) 286, 767, 1290, 1465
BspMI ACCTGC 2 cut(s) 238, 655
BspPI GGATC 3 cut(s) 1400, 1458, 1471
BspT104I TTCGAA 1 cut(s) 406
BspT107I GGYRCC 1 cut(s) 765
BsrDI GCAATG 1 cut(s) 1485
BsrI ACTGG 4 cut(s) 213, 288, 982, 1266
BssECI CCNNGG 1 cut(s) 928
BssMI GATC 5 cut(s) 186, 742, 952, 1392, 1463
BssT1I CCWWGG 1 cut(s) 928
Bst2UI CCWGG 1 cut(s) 461
Bst6I CTCTTC 1 cut(s) 1149
BstBI TTCGAA 1 cut(s) 406
BstC8I GCNNGC 6 cut(s) 349, 457, 673, 1117, 1181, 1411
BstDEI CTNAG 5 cut(s) 120, 480, 770, 1133, 1453
BstDSI CCRYGG 1 cut(s) 928
BstF5I GGATG 2 cut(s) 845, 1489
BstFNI CGCG 3 cut(s) 200, 326, 737
BstH2I RGCGCY 1 cut(s) 348
BstHHI GCGC 4 cut(s) 326, 347, 455, 737
BstKTI GATC 5 cut(s) 189, 745, 955, 1395, 1466
BstMAI GTCTC 2 cut(s) 89, 1069
BstMBI GATC 5 cut(s) 186, 742, 952, 1392, 1463
BstMCI CGRYCG 3 cut(s) 245, 628, 632
BstMWI GCNNNNNNNGC 6 cut(s) 292, 348, 363, 722, 902, 1189
BstNI CCWGG 1 cut(s) 461
BstNSI RCATGY 4 cut(s) 427, 826, 1119, 1183
BstSCI CCNGG 4 cut(s) 144, 459, 608, 1142
BstSFI CTRYAG 1 cut(s) 1414
BstSLI GKGCMC 1 cut(s) 288
BstUI CGCG 3 cut(s) 200, 326, 737
BstX2I RGATCY 1 cut(s) 1463
BstXI CCANNNNNNTGG 2 cut(s) 220, 935
BstYI RGATCY 1 cut(s) 1463
BstZI CGGCCG 1 cut(s) 629
Bsu36I CCTNAGG 1 cut(s) 770
BsuRI GGCC 6 cut(s) 286, 351, 366, 631, 1268, 1523
BtgI CCRYGG 1 cut(s) 928
BtgZI GCGATG 1 cut(s) 563
BtrI CACGTC 1 cut(s) 1079
BtsCI GGATG 2 cut(s) 845, 1489
BtsI GCAGTG 1 cut(s) 946
BtsIMutI CAGTG 2 cut(s) 220, 946
BveI ACCTGC 2 cut(s) 238, 655
Cac8I GCNNGC 6 cut(s) 349, 457, 673, 1117, 1181, 1411
CciI TCATGA 2 cut(s) 183, 655
CfoI GCGC 4 cut(s) 326, 347, 455, 737
Cfr13I GGNCC 3 cut(s) 284, 285, 1267
Csp6I GTAC 3 cut(s) 379, 427, 766
CviQI GTAC 3 cut(s) 379, 427, 766
DdeI CTNAG 5 cut(s) 120, 480, 770, 1133, 1453
DpnI GATC 5 cut(s) 188, 744, 954, 1394, 1465
DpnII GATC 5 cut(s) 186, 742, 952, 1392, 1463
EaeI YGGCCR 3 cut(s) 349, 364, 629
EagI CGGCCG 1 cut(s) 629
Eam1104I CTCTTC 1 cut(s) 1149
EarI CTCTTC 1 cut(s) 1149
EciI GGCGGA 1 cut(s) 1175
Ecl136II GAGCTC 1 cut(s) 10
EclXI CGGCCG 1 cut(s) 629
Eco130I CCWWGG 1 cut(s) 928
Eco147I AGGCCT 1 cut(s) 1523
Eco24I GRGCYC 2 cut(s) 12, 288
Eco52I CGGCCG 1 cut(s) 629
Eco53kI GAGCTC 1 cut(s) 10
Eco81I CCTNAGG 1 cut(s) 770
EcoICRI GAGCTC 1 cut(s) 10
EcoRI GAATTC 2 cut(s) 595, 1448
EcoRII CCWGG 1 cut(s) 459
EcoT14I CCWWGG 1 cut(s) 928
EcoT22I ATGCAT 2 cut(s) 1117, 1121
EcoT38I GRGCYC 2 cut(s) 12, 288
ErhI CCWWGG 1 cut(s) 928
Esp3I CGTCTC 2 cut(s) 89, 1069
FaqI GGGAC 2 cut(s) 25, 763
FauI CCCGC 1 cut(s) 524
FbaI TGATCA 2 cut(s) 186, 742
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 327, 343, 717
FokI GGATG 2 cut(s) 852, 1496
FriOI GRGCYC 2 cut(s) 12, 288
Fsp4HI GCNGC 3 cut(s) 327, 343, 717
FspBI CTAG 2 cut(s) 705, 1525
FspI TGCGCA 1 cut(s) 454
GlaI GCGC 4 cut(s) 325, 346, 454, 736
GluI GCNGC 3 cut(s) 327, 343, 717
GsuI CTGGAG 1 cut(s) 806
HaeII RGCGCY 1 cut(s) 348
HaeIII GGCC 6 cut(s) 286, 351, 366, 631, 1268, 1523
HapII CCGG 5 cut(s) 145, 352, 609, 686, 1143
HhaI GCGC 4 cut(s) 326, 347, 455, 737
Hin6I GCGC 4 cut(s) 324, 345, 453, 735
HinP1I GCGC 4 cut(s) 324, 345, 453, 735
HincII GTYRAC 2 cut(s) 820, 1315
HindII GTYRAC 2 cut(s) 820, 1315
HindIII AAGCTT 1 cut(s) 852
HinfI GANTC 4 cut(s) 110, 163, 991, 1504
HpaII CCGG 5 cut(s) 145, 352, 609, 686, 1143
Hpy166II GTNNAC 4 cut(s) 175, 820, 1084, 1315
Hpy188I TCNGA 5 cut(s) 109, 123, 191, 1296, 1460
Hpy188III TCNNGA 4 cut(s) 184, 656, 1445, 1501
Hpy8I GTNNAC 4 cut(s) 175, 820, 1084, 1315
HpyAV CCTTC 2 cut(s) 310, 628
HpyCH4IV ACGT 2 cut(s) 171, 1078
HpyCH4V TGCA 8 cut(s) 664, 671, 760, 1115, 1119, 1214, 1409, 1478
HpyF10VI GCNNNNNNNGC 6 cut(s) 292, 348, 363, 722, 902, 1189
HpyF3I CTNAG 5 cut(s) 120, 480, 770, 1133, 1453
HpySE526I ACGT 2 cut(s) 171, 1078
HspAI GCGC 4 cut(s) 324, 345, 453, 735
KpnI GGTACC 1 cut(s) 769
Ksp22I TGATCA 2 cut(s) 186, 742
Kzo9I GATC 5 cut(s) 186, 742, 952, 1392, 1463
LmnI GCTCC 1 cut(s) 354
MaeI CTAG 2 cut(s) 705, 1525
MaeII ACGT 2 cut(s) 171, 1078
MaeIII GTNAC 1 cut(s) 997
MalI GATC 5 cut(s) 188, 744, 954, 1394, 1465
MboI GATC 5 cut(s) 186, 742, 952, 1392, 1463
MboII GAAGA 5 cut(s) 85, 401, 577, 892, 1166
MflI RGATCY 1 cut(s) 1463
MhlI GDGCHC 5 cut(s) 12, 103, 288, 727, 1030
MlsI TGGCCA 1 cut(s) 366
MluCI AATT 6 cut(s) 595, 1089, 1149, 1278, 1365, 1448
MluI ACGCGT 1 cut(s) 198
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 2 cut(s) 157, 1513
MmeI TCCRAC 1 cut(s) 572
Mox20I TGGCCA 1 cut(s) 366
Mph1103I ATGCAT 2 cut(s) 1117, 1121
MroXI GAANNNNTTC 1 cut(s) 651
MscI TGGCCA 1 cut(s) 366
MseI TTAA 6 cut(s) 375, 867, 1088, 1277, 1350, 1368
MslI CAYNNNNRTG 2 cut(s) 199, 1184
Msp20I TGGCCA 1 cut(s) 366
MspI CCGG 5 cut(s) 145, 352, 609, 686, 1143
MspR9I CCNGG 4 cut(s) 146, 461, 610, 1144
Mva1269I GAATGC 1 cut(s) 326
MvaI CCWGG 1 cut(s) 461
MvnI CGCG 3 cut(s) 200, 326, 737
MwoI GCNNNNNNNGC 6 cut(s) 292, 348, 363, 722, 902, 1189
NciI CCSGG 3 cut(s) 146, 610, 1144
NcoI CCATGG 1 cut(s) 928
NdeII GATC 5 cut(s) 186, 742, 952, 1392, 1463
NlaIV GGNNCC 4 cut(s) 286, 767, 1290, 1465
NmuCI GTSAC 1 cut(s) 997
NsbI TGCGCA 1 cut(s) 454
NsiI ATGCAT 2 cut(s) 1117, 1121
NspI RCATGY 4 cut(s) 427, 826, 1119, 1183
NspV TTCGAA 1 cut(s) 406
PaeI GCATGC 2 cut(s) 1119, 1183
PagI TCATGA 2 cut(s) 183, 655
PceI AGGCCT 1 cut(s) 1523
PciI ACATGT 2 cut(s) 423, 822
PcsI WCGNNNNNNNCGW 2 cut(s) 242, 503
PctI GAATGC 1 cut(s) 326
PdmI GAANNNNTTC 1 cut(s) 651
PfeI GAWTC 2 cut(s) 110, 991
PkrI GCNGC 3 cut(s) 328, 344, 718
PleI GAGTC 2 cut(s) 157, 1512
PpsI GAGTC 2 cut(s) 157, 1512
PscI ACATGT 2 cut(s) 423, 822
PsiI TTATAA 1 cut(s) 605
Psp124BI GAGCTC 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 459
PspGI CCWGG 1 cut(s) 459
PspN4I GGNNCC 4 cut(s) 286, 767, 1290, 1465
PspOMI GGGCCC 1 cut(s) 284
PspPI GGNCC 3 cut(s) 284, 285, 1267
PsuI RGATCY 1 cut(s) 1463
RsaI GTAC 3 cut(s) 380, 428, 767
RsaNI GTAC 3 cut(s) 379, 427, 766
RseI CAYNNNNRTG 2 cut(s) 199, 1184
SacI GAGCTC 1 cut(s) 12
SaqAI TTAA 6 cut(s) 375, 867, 1088, 1277, 1350, 1368
SatI GCNGC 3 cut(s) 327, 343, 717
Sau3AI GATC 5 cut(s) 186, 742, 952, 1392, 1463
Sau96I GGNCC 3 cut(s) 284, 285, 1267
SchI GAGTC 2 cut(s) 157, 1513
ScrFI CCNGG 4 cut(s) 146, 461, 610, 1144
SduI GDGCHC 5 cut(s) 12, 103, 288, 727, 1030
SfcI CTRYAG 1 cut(s) 1414
SfuI TTCGAA 1 cut(s) 406
SmiMI CAYNNNNRTG 2 cut(s) 199, 1184
SmlI CTYRAG 1 cut(s) 1501
SmoI CTYRAG 1 cut(s) 1501
SphI GCATGC 2 cut(s) 1119, 1183
Sse9I AATT 6 cut(s) 595, 1089, 1149, 1278, 1365, 1448
SseBI AGGCCT 1 cut(s) 1523
SsiI CCGC 9 cut(s) 135, 326, 342, 531, 624, 675, 716, 737, 1160
SspMI CTAG 2 cut(s) 705, 1525
SstI GAGCTC 1 cut(s) 12
StuI AGGCCT 1 cut(s) 1523
StyD4I CCNGG 4 cut(s) 144, 459, 608, 1142
StyI CCWWGG 1 cut(s) 928
TaiI ACGT 2 cut(s) 174, 1081
TaqI TCGA 3 cut(s) 406, 506, 1431
TaqII GACCGA 1 cut(s) 231
TasI AATT 6 cut(s) 595, 1089, 1149, 1278, 1365, 1448
TatI WGTACW 1 cut(s) 426
TauI GCSGC 3 cut(s) 329, 345, 719
TfiI GAWTC 2 cut(s) 110, 991
Tru1I TTAA 6 cut(s) 375, 867, 1088, 1277, 1350, 1368
Tru9I TTAA 6 cut(s) 375, 867, 1088, 1277, 1350, 1368
TscAI CASTG 2 cut(s) 220, 946
TseFI GTSAC 1 cut(s) 997
Tsp45I GTSAC 1 cut(s) 997
TspDTI ATGAA 9 cut(s) 350, 434, 486, 584, 644, 672, 1202, 1394, 1500
TspGWI ACGGA 1 cut(s) 294
TspRI CASTG 2 cut(s) 220, 946
XapI RAATTY 2 cut(s) 595, 1448
XceI RCATGY 4 cut(s) 427, 826, 1119, 1183
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 651
XspI CTAG 2 cut(s) 705, 1525
Zsp2I ATGCAT 2 cut(s) 1117, 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.