Rh1BG366800

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0011997
Physical Location & Seq
Forward (+)
49667672 .. 49669249
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG366800.1

Sequence Viewer

Length: 1578 bp
ATGTCGAGTCTTAGAACCACACTCTTACACCTACTCGAAGAATGCAAGAACATGAGAGAGCTGAAACAAATCCACACCCACATCATAAAGTCCCCATTTTTACTCAAACAAGACCAATCCTTTCTCATCACTCGTCTCCTATTCTTCTGCGCTCTATCCGATTCGGGTTCTCAGACCTACGCCACCGGAGTTTTCCGGGTCATAAAGAACCCGACTCTCTACGTCTACAACATCATGATCAGAGCACACGCGTGTAGAAAGACCAGTGCTTTGGATAAACCTGCTTCGTTTCGGTCGTTGGTGTTGTATAAGCAAATGGTTGGTAATGGGATTGGGCCCAATTGCCTCACTTTTCCGTTTCTTGTGAAGGAATGCGCGGCGAGGGTTGACGGCGGCGCTGGCCGGAGCTTTCATGGTCAGGTGATTAAGTACGGGTTTGGAGATGATGTGTTTGTTCGAAACTCGTTGATGAATATGTACTTTGTTTGTGGGTCTTTGAGTTGCGCACGCAAGGTGTTCGATGAAATTCCTGAGAGAGATGTGGTTTCGTGGAACTTGATGATTAAGGGGTGTTTGAGAAGTGGGGAACTTGATGAGGCGATGGGTGTGTTTGGGAAGATGAAGCAGAGGAGCATTTTTAGTTGGAATTCCATTATAACCGGGTGTGTTCAAGGCGGTCGGCCGAAGGAGGCATTGGAACTTTTTCATGAAATGCAGGTTGCGGGCGGTGTTAGACCGGATAAGATTACGATTGCTAGTGTCCTTGCGGCTTGTGCTCATCTTGGCGCGGTTGATCACGGGACATGGGTGCATGGGTGCCTGAGGAGAAGTGGGCTGGAGAGTGATGTGGTTATTGGAACAGCTCTGGTTGACATGTATGGTAAATGTGGATGTGTGGAAAAAGCTTATGAGGTCTTTAAGGAAATGCCGAAGAAAGATACTTTGGCATGGACAGCTATGATTTCGGTTTTTGCTCTCCATGGGTTTGGCAATGAGGCTTTTGATCTTTTTGAAAAGATGGAAGCAACTGGGGTTGAGCCGAATCATGTGACGTTTGTTGGGTTGTTGTCAGCTTGTGCTCATTCCGGTTTAGTAGAGAAAGGTCGCTGGTGTTTTGATGTGATGAGACGTGTTTACTTAATTGAACCACAGCTTTATCATTATGCATGCATGGTTGATATACTTAGCAGAGCCGGGCTAATTGAAGAGGCGGAGAGGTTGATAAGAGGCATGCCAATGAAGCCAGATGTATTTGTTTGGGGTGCATTACTTGGAGGTTGTCAAATACATGGAAAGGTTGAGATAGGAGAACTGGTGGCCCGTCATTTAATTGATATGGAACCTCTGAACCATGCTTTTTATGTCAACTTGTGCGATATATATGCCAAAGCTGGTAGATTTAATGATGTGAAAACAATTAAATCACTTATGAAAGAAAGAGGGATCAAAAAGGAAGTTGCAGGCTGTAGCCTGATTGAAGTCGATGGAGTTGTTCTTGAATTCTCAGTCAGAGGATCCCCTGATGTTGTAATGGATGAAGTATTATGGGTCTTGAGTCAGTTGAATAGTGAGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

525

Amino Acids

58.4

Weight (kDa)

7.8

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 179 - 212 5.2e-08 PPR repeat family
PPR PF01535 182 - 211 2.1e-08 PPR repeat
PPR_2 PF13041 211 - 260 1.5e-09 PPR repeat family
PPR PF01535 213 - 242 3.2e-08 PPR repeat
PPR_2 PF13041 313 - 361 5e-10 PPR repeat family
E_motif PF20431 430 - 493 7e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14170 AT4G14170
fragaria_vesca FvH4_7g28451
malus_domestica MD01G1188300.v1.1
prunus_persica Prupe.2G284600_v2.0.a1
pyrus_communis pycom01g20010
rosa_chinensis RchiOBHm_Chr1g0375631 RchiOBHm_Chr1g0375671
rosa_laevigata RLG00000026661
rosa_multiflora Rmu_co8281021.1_g000001 Rmu_co8425983.1_g000001 Rmu_sc0001644.1_g000022
rosa_roxburghii Rroxscaffold_4G00282680
rosa_rugosa Rorug01G0391700
rosa_samantha Rh1AG402600 Rh1BG366800 Rh1CG379500
rosa_wichuraiana Rw1G035750 Rw1G035770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 656
Acc16I TGCGCA 1 cut(s) 505
Acc36I ACCTGC 2 cut(s) 289, 706
AccB1I GGYRCC 1 cut(s) 816
AccI GTMKAC 1 cut(s) 225
AccII CGCG 3 cut(s) 251, 377, 788
AciI CCGC 8 cut(s) 377, 393, 675, 722, 726, 767, 788, 1211
AclWI GGATC 3 cut(s) 1451, 1509, 1522
AcoI YGGCCR 2 cut(s) 400, 680
AcsI RAATTY 3 cut(s) 525, 646, 1499
AfaI GTAC 2 cut(s) 431, 479
AflIII ACRYGT 3 cut(s) 249, 873, 1129
AgsI TTSAA 7 cut(s) 671, 1013, 1145, 1205, 1478, 1499, 1564
AjiI CACGTC 1 cut(s) 1130
AjuI GAANNNNNNNTTGG 4 cut(s) 677, 709, 926, 958
AleI CACNNNNGTG 1 cut(s) 250
AluBI AGCT 8 cut(s) 61, 408, 863, 905, 956, 1073, 1153, 1391
AluI AGCT 8 cut(s) 61, 408, 863, 905, 956, 1073, 1153, 1391
Alw21I GWGCWC 3 cut(s) 247, 778, 1081
Alw26I GTCTC 2 cut(s) 140, 1120
AlwI GGATC 3 cut(s) 1451, 1509, 1522
AoxI GGCC 5 cut(s) 335, 400, 680, 1317, 1572
ApaI GGGCCC 1 cut(s) 339
ApoI RAATTY 3 cut(s) 525, 646, 1499
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 1 cut(s) 702
AspLEI GCGC 5 cut(s) 152, 377, 398, 506, 788
AspS9I GGNCC 3 cut(s) 335, 336, 1318
AsuC2I CCSGG 3 cut(s) 197, 661, 1195
AsuHPI GGTGA 1 cut(s) 433
AsuII TTCGAA 1 cut(s) 457
AxyI CCTNAGG 1 cut(s) 821
BaeGI GKGCMC 1 cut(s) 339
BamHI GGATCC 1 cut(s) 1514
BanI GGYRCC 1 cut(s) 816
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 3 cut(s) 247, 778, 1081
BccI CCATC 3 cut(s) 595, 1012, 1478
BceAI ACGGC 1 cut(s) 406
BcgI CGANNNNNNTGC 4 cut(s) 499, 533, 1364, 1398
BclI TGATCA 2 cut(s) 237, 793
BcnI CCSGG 3 cut(s) 197, 661, 1195
BcoDI GTCTC 2 cut(s) 140, 1120
BfaI CTAG 2 cut(s) 756, 1576
BfmI CTRYAG 1 cut(s) 1465
BfoI RGCGCY 1 cut(s) 399
BfuAI ACCTGC 2 cut(s) 289, 706
BisI GCNGC 3 cut(s) 378, 394, 768
BlsI GCNGC 3 cut(s) 379, 395, 769
Bme1390I CCNGG 3 cut(s) 197, 661, 1195
BmgBI CACGTC 1 cut(s) 1130
BmgT120I GGNCC 3 cut(s) 335, 336, 1318
BmiI GGNNCC 4 cut(s) 337, 818, 1341, 1516
BmrFI CCNGG 3 cut(s) 197, 661, 1195
BmrI ACTGGG 1 cut(s) 1038
BmuI ACTGGG 1 cut(s) 1038
BpmI CTGGAG 1 cut(s) 857
Bpu14I TTCGAA 1 cut(s) 457
BpuEI CTTGAG 1 cut(s) 1573
BpuMI CCSGG 3 cut(s) 197, 661, 1195
BsaJI CCNNGG 1 cut(s) 979
BsaWI WCCGGW 3 cut(s) 185, 736, 1085
BsaXI ACNNNNNCTCC 4 cut(s) 432, 462, 1299, 1329
Bse1I ACTGG 3 cut(s) 264, 1033, 1317
Bse21I CCTNAGG 1 cut(s) 821
Bse3DI GCAATG 1 cut(s) 997
BseDI CCNNGG 1 cut(s) 979
BseGI GGATG 2 cut(s) 896, 1540
BseMI GCAATG 1 cut(s) 997
BseMII CTCAG 4 cut(s) 185, 522, 812, 1518
BseNI ACTGG 3 cut(s) 264, 1033, 1317
BseRI GAGGAG 2 cut(s) 643, 838
BseSI GKGCMC 1 cut(s) 339
BseX3I CGGCCG 1 cut(s) 680
Bsh1236I CGCG 3 cut(s) 251, 377, 788
Bsh1285I CGRYCG 3 cut(s) 296, 679, 683
BshFI GGCC 5 cut(s) 337, 402, 682, 1319, 1574
BshNI GGYRCC 1 cut(s) 816
BsiEI CGRYCG 3 cut(s) 296, 679, 683
BsiHKAI GWGCWC 3 cut(s) 247, 778, 1081
BsiSI CCGG 7 cut(s) 186, 196, 403, 660, 737, 1086, 1194
BslFI GGGAC 2 cut(s) 76, 814
BsmAI GTCTC 2 cut(s) 140, 1120
BsmBI CGTCTC 2 cut(s) 140, 1120
BsmFI GGGAC 2 cut(s) 76, 814
BsmI GAATGC 2 cut(s) 47, 377
BsnI GGCC 5 cut(s) 337, 402, 682, 1319, 1574
Bsp119I TTCGAA 1 cut(s) 457
Bsp120I GGGCCC 1 cut(s) 335
Bsp1286I GDGCHC 4 cut(s) 247, 339, 778, 1081
Bsp143I GATC 5 cut(s) 237, 793, 1003, 1443, 1514
Bsp19I CCATGG 1 cut(s) 979
BspACI CCGC 8 cut(s) 377, 393, 675, 722, 726, 767, 788, 1211
BspANI GGCC 5 cut(s) 337, 402, 682, 1319, 1574
BspCNI CTCAG 4 cut(s) 184, 523, 813, 1517
BspFNI CGCG 3 cut(s) 251, 377, 788
BspHI TCATGA 2 cut(s) 234, 706
BspLI GGNNCC 4 cut(s) 337, 818, 1341, 1516
BspMI ACCTGC 2 cut(s) 289, 706
BspPI GGATC 3 cut(s) 1451, 1509, 1522
BspT104I TTCGAA 1 cut(s) 457
BspT107I GGYRCC 1 cut(s) 816
BsrDI GCAATG 1 cut(s) 997
BsrI ACTGG 3 cut(s) 264, 1033, 1317
BssECI CCNNGG 1 cut(s) 979
BssMI GATC 5 cut(s) 237, 793, 1003, 1443, 1514
BssT1I CCWWGG 1 cut(s) 979
Bst6I CTCTTC 1 cut(s) 1200
BstBI TTCGAA 1 cut(s) 457
BstC8I GCNNGC 6 cut(s) 400, 508, 724, 1168, 1232, 1462
BstDEI CTNAG 6 cut(s) 11, 171, 531, 821, 1184, 1504
BstDSI CCRYGG 1 cut(s) 979
BstF5I GGATG 2 cut(s) 896, 1540
BstFNI CGCG 3 cut(s) 251, 377, 788
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 5 cut(s) 152, 377, 398, 506, 788
BstKTI GATC 5 cut(s) 240, 796, 1006, 1446, 1517
BstMAI GTCTC 2 cut(s) 140, 1120
BstMBI GATC 5 cut(s) 237, 793, 1003, 1443, 1514
BstMCI CGRYCG 3 cut(s) 296, 679, 683
BstMWI GCNNNNNNNGC 4 cut(s) 399, 773, 953, 1240
BstNSI RCATGY 3 cut(s) 877, 1170, 1234
BstSCI CCNGG 3 cut(s) 195, 659, 1193
BstSFI CTRYAG 1 cut(s) 1465
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 3 cut(s) 251, 377, 788
BstX2I RGATCY 1 cut(s) 1514
BstXI CCANNNNNNTGG 2 cut(s) 271, 986
BstYI RGATCY 1 cut(s) 1514
BstZI CGGCCG 1 cut(s) 680
Bsu36I CCTNAGG 1 cut(s) 821
BsuRI GGCC 5 cut(s) 337, 402, 682, 1319, 1574
BtgI CCRYGG 1 cut(s) 979
BtgZI GCGATG 1 cut(s) 614
BtrI CACGTC 1 cut(s) 1130
BtsCI GGATG 2 cut(s) 896, 1540
BtsIMutI CAGTG 1 cut(s) 271
BveI ACCTGC 2 cut(s) 289, 706
Cac8I GCNNGC 6 cut(s) 400, 508, 724, 1168, 1232, 1462
CciI TCATGA 2 cut(s) 234, 706
CfoI GCGC 5 cut(s) 152, 377, 398, 506, 788
Cfr13I GGNCC 3 cut(s) 335, 336, 1318
Csp6I GTAC 2 cut(s) 430, 478
CviQI GTAC 2 cut(s) 430, 478
DdeI CTNAG 6 cut(s) 11, 171, 531, 821, 1184, 1504
DpnI GATC 5 cut(s) 239, 795, 1005, 1445, 1516
DpnII GATC 5 cut(s) 237, 793, 1003, 1443, 1514
EaeI YGGCCR 2 cut(s) 400, 680
EagI CGGCCG 1 cut(s) 680
Eam1104I CTCTTC 1 cut(s) 1200
EarI CTCTTC 1 cut(s) 1200
EciI GGCGGA 1 cut(s) 1226
EclXI CGGCCG 1 cut(s) 680
Eco130I CCWWGG 1 cut(s) 979
Eco147I AGGCCT 1 cut(s) 1574
Eco24I GRGCYC 1 cut(s) 339
Eco52I CGGCCG 1 cut(s) 680
Eco81I CCTNAGG 1 cut(s) 821
EcoRI GAATTC 2 cut(s) 646, 1499
EcoT14I CCWWGG 1 cut(s) 979
EcoT22I ATGCAT 2 cut(s) 1168, 1172
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 979
Esp3I CGTCTC 2 cut(s) 140, 1120
FaqI GGGAC 2 cut(s) 76, 814
FauI CCCGC 1 cut(s) 715
FbaI TGATCA 2 cut(s) 237, 793
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 3 cut(s) 378, 394, 768
FokI GGATG 2 cut(s) 903, 1547
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 3 cut(s) 378, 394, 768
FspBI CTAG 2 cut(s) 756, 1576
FspI TGCGCA 1 cut(s) 505
GlaI GCGC 5 cut(s) 151, 376, 397, 505, 787
GluI GCNGC 3 cut(s) 378, 394, 768
GsuI CTGGAG 1 cut(s) 857
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 5 cut(s) 337, 402, 682, 1319, 1574
HapII CCGG 7 cut(s) 186, 196, 403, 660, 737, 1086, 1194
HhaI GCGC 5 cut(s) 152, 377, 398, 506, 788
Hin6I GCGC 5 cut(s) 150, 375, 396, 504, 786
HinP1I GCGC 5 cut(s) 150, 375, 396, 504, 786
HincII GTYRAC 3 cut(s) 388, 871, 1366
HindII GTYRAC 3 cut(s) 388, 871, 1366
HindIII AAGCTT 1 cut(s) 903
HinfI GANTC 5 cut(s) 7, 161, 214, 1042, 1555
HpaII CCGG 7 cut(s) 186, 196, 403, 660, 737, 1086, 1194
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 5 cut(s) 226, 388, 871, 1135, 1366
Hpy188I TCNGA 5 cut(s) 160, 174, 242, 1347, 1511
Hpy188III TCNNGA 5 cut(s) 235, 530, 707, 1496, 1552
Hpy8I GTNNAC 5 cut(s) 226, 388, 871, 1135, 1366
HpyAV CCTTC 2 cut(s) 361, 679
HpyCH4IV ACGT 3 cut(s) 222, 1052, 1129
HpyCH4V TGCA 7 cut(s) 45, 715, 811, 1166, 1170, 1265, 1460
HpyF10VI GCNNNNNNNGC 4 cut(s) 399, 773, 953, 1240
HpyF3I CTNAG 6 cut(s) 11, 171, 531, 821, 1184, 1504
HpySE526I ACGT 3 cut(s) 222, 1052, 1129
HspAI GCGC 5 cut(s) 150, 375, 396, 504, 786
Ksp22I TGATCA 2 cut(s) 237, 793
Kzo9I GATC 5 cut(s) 237, 793, 1003, 1443, 1514
LmnI GCTCC 2 cut(s) 405, 630
MaeI CTAG 2 cut(s) 756, 1576
MaeII ACGT 3 cut(s) 222, 1052, 1129
MaeIII GTNAC 1 cut(s) 1048
MalI GATC 5 cut(s) 239, 795, 1005, 1445, 1516
MboI GATC 5 cut(s) 237, 793, 1003, 1443, 1514
MboII GAAGA 5 cut(s) 50, 136, 628, 943, 1217
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 1 cut(s) 1514
MhlI GDGCHC 4 cut(s) 247, 339, 778, 1081
MluCI AATT 8 cut(s) 340, 525, 646, 1140, 1200, 1329, 1416, 1499
MluI ACGCGT 1 cut(s) 249
MlyI GAGTC 3 cut(s) 16, 208, 1564
MmeI TCCRAC 1 cut(s) 623
Mph1103I ATGCAT 2 cut(s) 1168, 1172
MroXI GAANNNNTTC 1 cut(s) 702
MseI TTAA 7 cut(s) 426, 564, 918, 1139, 1328, 1401, 1419
MslI CAYNNNNRTG 2 cut(s) 250, 1235
MspI CCGG 7 cut(s) 186, 196, 403, 660, 737, 1086, 1194
MspR9I CCNGG 3 cut(s) 197, 661, 1195
MunI CAATTG 1 cut(s) 340
Mva1269I GAATGC 2 cut(s) 47, 377
MvnI CGCG 3 cut(s) 251, 377, 788
MwoI GCNNNNNNNGC 4 cut(s) 399, 773, 953, 1240
NciI CCSGG 3 cut(s) 197, 661, 1195
NcoI CCATGG 1 cut(s) 979
NdeII GATC 5 cut(s) 237, 793, 1003, 1443, 1514
NlaIV GGNNCC 4 cut(s) 337, 818, 1341, 1516
NmuCI GTSAC 1 cut(s) 1048
NsbI TGCGCA 1 cut(s) 505
NsiI ATGCAT 2 cut(s) 1168, 1172
NspI RCATGY 3 cut(s) 877, 1170, 1234
NspV TTCGAA 1 cut(s) 457
OliI CACNNNNGTG 1 cut(s) 250
PaeI GCATGC 2 cut(s) 1170, 1234
PagI TCATGA 2 cut(s) 234, 706
PceI AGGCCT 1 cut(s) 1574
PciI ACATGT 1 cut(s) 873
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PctI GAATGC 2 cut(s) 47, 377
PdmI GAANNNNTTC 1 cut(s) 702
PfeI GAWTC 2 cut(s) 161, 1042
PkrI GCNGC 3 cut(s) 379, 395, 769
PleI GAGTC 3 cut(s) 15, 208, 1563
PpsI GAGTC 3 cut(s) 15, 208, 1563
PscI ACATGT 1 cut(s) 873
PsiI TTATAA 1 cut(s) 656
PspN4I GGNNCC 4 cut(s) 337, 818, 1341, 1516
PspOMI GGGCCC 1 cut(s) 335
PspPI GGNCC 3 cut(s) 335, 336, 1318
PsuI RGATCY 1 cut(s) 1514
RsaI GTAC 2 cut(s) 431, 479
RsaNI GTAC 2 cut(s) 430, 478
RseI CAYNNNNRTG 2 cut(s) 250, 1235
SaqAI TTAA 7 cut(s) 426, 564, 918, 1139, 1328, 1401, 1419
SatI GCNGC 3 cut(s) 378, 394, 768
Sau3AI GATC 5 cut(s) 237, 793, 1003, 1443, 1514
Sau96I GGNCC 3 cut(s) 335, 336, 1318
SchI GAGTC 3 cut(s) 16, 208, 1564
ScrFI CCNGG 3 cut(s) 197, 661, 1195
SduI GDGCHC 4 cut(s) 247, 339, 778, 1081
SfcI CTRYAG 1 cut(s) 1465
SfuI TTCGAA 1 cut(s) 457
SmiMI CAYNNNNRTG 2 cut(s) 250, 1235
SmlI CTYRAG 1 cut(s) 1552
SmoI CTYRAG 1 cut(s) 1552
SphI GCATGC 2 cut(s) 1170, 1234
Sse9I AATT 8 cut(s) 340, 525, 646, 1140, 1200, 1329, 1416, 1499
SseBI AGGCCT 1 cut(s) 1574
SsiI CCGC 8 cut(s) 377, 393, 675, 722, 726, 767, 788, 1211
SspMI CTAG 2 cut(s) 756, 1576
StuI AGGCCT 1 cut(s) 1574
StyD4I CCNGG 3 cut(s) 195, 659, 1193
StyI CCWWGG 1 cut(s) 979
TaiI ACGT 3 cut(s) 225, 1055, 1132
TaqI TCGA 5 cut(s) 5, 36, 457, 519, 1482
TaqII GACCGA 1 cut(s) 282
TasI AATT 8 cut(s) 340, 525, 646, 1140, 1200, 1329, 1416, 1499
TatI WGTACW 1 cut(s) 477
TauI GCSGC 3 cut(s) 380, 396, 770
TfiI GAWTC 2 cut(s) 161, 1042
Tru1I TTAA 7 cut(s) 426, 564, 918, 1139, 1328, 1401, 1419
Tru9I TTAA 7 cut(s) 426, 564, 918, 1139, 1328, 1401, 1419
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 1 cut(s) 1048
Tsp45I GTSAC 1 cut(s) 1048
TspDTI ATGAA 9 cut(s) 401, 485, 537, 635, 695, 723, 1253, 1445, 1551
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 1 cut(s) 271
XapI RAATTY 3 cut(s) 525, 646, 1499
XceI RCATGY 3 cut(s) 877, 1170, 1234
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 702
XspI CTAG 2 cut(s) 756, 1576
Zsp2I ATGCAT 2 cut(s) 1168, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.