AT4G15620

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014303
Physical Location & Seq
Forward (+)
8913653 .. 8915663
2011 bp
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UTR
Exon/CDS
Intron
AT4G15620.1

Sequence Viewer

Length: 573 bp
ATGGAACACGAGGGCAAGAACAACATGAATGGTATGGAGATGGAGAAAGGAAAGAGAGAGCTTGGGTCAAGAAAAGGAGTTGAGTTAACTATGAGAGTGTTGGCTTTGATCCTAACAATGGCGGCTGCAACAGTACTTGGTGTCGCAAAGCAAACAAAGGTTGTGTCTATTAAGCTTATTCCCACTTTGCCTCCTCTTGATATCACTACTACCGCCAAAGCCTCTTACTTGTCGGCATTTGTGTACAACATTTCTGTGAACGCTATAGCTTGCGGTTACACGGCGATCTCGATAGCGATTCTGATGATCAGCAGGGGAAGAAGAAGCAAGAAGTTGTTAATGGTGGTTTTGCTAGGAGATCTAGTGATGGTGGCTTTGCTGTTTTCCGGCACTGGAGCCGCCAGCGCAATTGGGCTAATGGGTTTACATGGGAACAAGCATGTCATGTGGAAGAAAGTTTGTGGCGTTTTTGGAAAATTCTGTCACCGAGCTGCTCCTTCGCTTCCTCTCACTCTTCTTGCTGCAGTTGTGTTTATGTTTCTTGTTGTTCTTGACGCTATTAAGCTTCCTTAA

Protein Analysis

190

Amino Acids

20.31

Weight (kDa)

10.02

Isoelectric Point (pI)

25.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 25 - 177 8.5e-37 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15620 AT4G15630
fragaria_vesca FvH4_4g17290
malus_domestica MD13G1173100.v1.1
prunus_persica Prupe.1G141500_v2.0.a1
pyrus_communis pycom13g14870 pycom16g14610
rosa_chinensis RchiOBHm_Chr4g0421251
rosa_laevigata RLG00000007706
rosa_multiflora Rmu_sc0009290.1_g000018
rosa_roxburghii Rroxscaffold_5G00363490
rosa_rugosa Rorug04G0169700
rosa_samantha Rh4AG229900 Rh4BG232600 Rh4CG244400 Rh4DG228600
rosa_wichuraiana Rw4G020370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 122, 213, 273, 399
AclWI GGATC 1 cut(s) 103
AcsI RAATTY 1 cut(s) 476
AfaI GTAC 2 cut(s) 135, 245
AfiI CCNNNNNNNGG 1 cut(s) 118
AluBI AGCT 5 cut(s) 61, 175, 269, 491, 565
AluI AGCT 5 cut(s) 61, 175, 269, 491, 565
AlwI GGATC 1 cut(s) 103
ApeKI GCWGC 3 cut(s) 125, 491, 521
ApoI RAATTY 1 cut(s) 476
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspLEI GCGC 1 cut(s) 407
AsuHPI GGTGA 1 cut(s) 476
BauI CACGAG 1 cut(s) 8
BbvI GCAGC 3 cut(s) 112, 478, 508
BccI CCATC 2 cut(s) 34, 361
BceAI ACGGC 1 cut(s) 297
BclI TGATCA 1 cut(s) 306
BfaI CTAG 2 cut(s) 353, 362
BfmI CTRYAG 2 cut(s) 264, 522
BglII AGATCT 1 cut(s) 358
BisI GCNGC 5 cut(s) 123, 126, 399, 492, 522
BlsI GCNGC 5 cut(s) 124, 127, 400, 493, 523
BmcAI AGTACT 1 cut(s) 135
BmiI GGNNCC 1 cut(s) 397
BpmI CTGGAG 1 cut(s) 414
BsaXI ACNNNNNCTCC 4 cut(s) 175, 205, 348, 378
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 397
BseLI CCNNNNNNNGG 1 cut(s) 118
BseNI ACTGG 1 cut(s) 397
BseRI GAGGAG 1 cut(s) 183
BseXI GCAGC 3 cut(s) 112, 478, 508
BsiSI CCGG 1 cut(s) 387
BslI CCNNNNNNNGG 1 cut(s) 118
Bsp1407I TGTACA 1 cut(s) 243
Bsp143I GATC 4 cut(s) 108, 285, 306, 358
BspACI CCGC 4 cut(s) 122, 213, 273, 399
BspLI GGNNCC 1 cut(s) 397
BspMAI CTGCAG 1 cut(s) 526
BspPI GGATC 1 cut(s) 103
BsrGI TGTACA 1 cut(s) 243
BsrI ACTGG 1 cut(s) 397
BssMI GATC 4 cut(s) 108, 285, 306, 358
BssSI CACGAG 1 cut(s) 8
Bst2BI CACGAG 1 cut(s) 8
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 1 cut(s) 519
BstAUI TGTACA 1 cut(s) 243
BstC8I GCNNGC 2 cut(s) 271, 403
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 4 cut(s) 111, 288, 309, 361
BstMBI GATC 4 cut(s) 108, 285, 306, 358
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstNSI RCATGY 1 cut(s) 443
BstSFI CTRYAG 2 cut(s) 264, 522
BstV1I GCAGC 3 cut(s) 112, 478, 508
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BtsIMutI CAGTG 1 cut(s) 390
Cac8I GCNNGC 2 cut(s) 271, 403
CfoI GCGC 1 cut(s) 407
CseI GACGC 1 cut(s) 563
Csp6I GTAC 2 cut(s) 134, 244
CviAII CATG 4 cut(s) 25, 428, 440, 445
CviQI GTAC 2 cut(s) 134, 244
DpnI GATC 4 cut(s) 110, 287, 308, 360
DpnII GATC 4 cut(s) 108, 285, 306, 358
Eam1104I CTCTTC 1 cut(s) 519
EarI CTCTTC 1 cut(s) 519
Eco32I GATATC 1 cut(s) 202
EcoRV GATATC 1 cut(s) 202
FaeI CATG 4 cut(s) 28, 431, 443, 448
FaiI YATR 8 cut(s) 26, 35, 92, 266, 429, 441, 446, 536
FatI CATG 4 cut(s) 24, 427, 439, 444
FbaI TGATCA 1 cut(s) 306
Fnu4HI GCNGC 5 cut(s) 123, 126, 399, 492, 522
Fsp4HI GCNGC 5 cut(s) 123, 126, 399, 492, 522
FspBI CTAG 2 cut(s) 353, 362
GlaI GCGC 1 cut(s) 406
GluI GCNGC 5 cut(s) 123, 126, 399, 492, 522
GsuI CTGGAG 1 cut(s) 414
HapII CCGG 1 cut(s) 387
HgaI GACGC 1 cut(s) 563
HhaI GCGC 1 cut(s) 407
Hin1II CATG 4 cut(s) 28, 431, 443, 448
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HindIII AAGCTT 2 cut(s) 173, 563
HinfI GANTC 1 cut(s) 298
HpaI GTTAAC 1 cut(s) 87
HpaII CCGG 1 cut(s) 387
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 4 cut(s) 87, 244, 259, 425
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 4 cut(s) 69, 197, 289, 551
Hpy8I GTNNAC 4 cut(s) 87, 244, 259, 425
HpyAV CCTTC 1 cut(s) 507
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4V TGCA 2 cut(s) 128, 524
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
Hsp92II CATG 4 cut(s) 28, 431, 443, 448
HspAI GCGC 1 cut(s) 405
Ksp22I TGATCA 1 cut(s) 306
KspAI GTTAAC 1 cut(s) 87
Kzo9I GATC 4 cut(s) 108, 285, 306, 358
LmnI GCTCC 2 cut(s) 395, 499
LpnPI CCDG 4 cut(s) 298, 378, 400, 415
Lsp1109I GCAGC 3 cut(s) 112, 478, 508
MaeI CTAG 2 cut(s) 353, 362
MaeIII GTNAC 2 cut(s) 275, 482
MalI GATC 4 cut(s) 110, 287, 308, 360
MboI GATC 4 cut(s) 108, 285, 306, 358
MboII GAAGA 4 cut(s) 330, 333, 463, 506
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 1 cut(s) 358
MluCI AATT 2 cut(s) 408, 476
MnlI CCTC 5 cut(s) 4, 201, 204, 232, 516
MseI TTAA 5 cut(s) 86, 171, 338, 561, 571
MslI CAYNNNNRTG 1 cut(s) 254
MspI CCGG 1 cut(s) 387
MunI CAATTG 1 cut(s) 408
MwoI GCNNNNNNNGC 1 cut(s) 404
NdeII GATC 4 cut(s) 108, 285, 306, 358
NlaIII CATG 4 cut(s) 28, 431, 443, 448
NlaIV GGNNCC 1 cut(s) 397
NmuCI GTSAC 1 cut(s) 482
NspI RCATGY 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 287
PfeI GAWTC 1 cut(s) 298
PkrI GCNGC 5 cut(s) 124, 127, 400, 493, 523
PspN4I GGNNCC 1 cut(s) 397
PstI CTGCAG 1 cut(s) 526
PsuI RGATCY 1 cut(s) 358
RsaI GTAC 2 cut(s) 135, 245
RsaNI GTAC 2 cut(s) 134, 244
RseI CAYNNNNRTG 1 cut(s) 254
SaqAI TTAA 5 cut(s) 86, 171, 338, 561, 571
SatI GCNGC 5 cut(s) 123, 126, 399, 492, 522
Sau3AI GATC 4 cut(s) 108, 285, 306, 358
ScaI AGTACT 1 cut(s) 135
SetI ASST 6 cut(s) 63, 162, 177, 271, 493, 567
SfcI CTRYAG 2 cut(s) 264, 522
SmiMI CAYNNNNRTG 1 cut(s) 254
Sse9I AATT 2 cut(s) 408, 476
SsiI CCGC 4 cut(s) 122, 213, 273, 399
SspMI CTAG 2 cut(s) 353, 362
TaaI ACNGT 1 cut(s) 133
TaqI TCGA 1 cut(s) 290
TasI AATT 2 cut(s) 408, 476
TatI WGTACW 2 cut(s) 133, 243
TauI GCSGC 2 cut(s) 125, 401
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 5 cut(s) 86, 171, 338, 561, 571
Tru9I TTAA 5 cut(s) 86, 171, 338, 561, 571
TscAI CASTG 1 cut(s) 397
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 3 cut(s) 125, 491, 521
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 1 cut(s) 41
TspRI CASTG 1 cut(s) 397
XapI RAATTY 1 cut(s) 476
XceI RCATGY 1 cut(s) 443
XspI CTAG 2 cut(s) 353, 362
ZrmI AGTACT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.