pycom13g14870

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014303
Physical Location & Seq
Reverse (-)
10754923 .. 10757181
2259 bp
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UTR
Exon/CDS
Intron
pycom13g14870.1

Sequence Viewer

Length: 573 bp
ATGGAGAGCCAGTACATTGGGAGCACTAGTGGGATGGAACAGCAGAAGGAAGTGAAGATAGCAAACCAAAGAGGAGGTGGGACACGTGAGTCGATTCTGAGGATTTTGGCCTTGGTGCTAACTCTTACGGCTTCTATTGTCCTGGGAGTCGGTAAGCAAACCGAAATTGTATCGATAAAGCTCATCCCAACCTTGCCTCCTATTGATGTTCCTGCTACGGCTAAGTGGCATTACTTATCTGCTTTTGTGTACCTTGTGGTAGCAGATGCCATAGCATGCACATATGCAGCATTTTCCCTAGTCCTTTCTTTTGCGAATCGGGGCACGAAGAATAAGTTAGGGCTGGTGATCGATCGTGCTCGATCTGTCAATGGTGGCGTTGCTCTTTTCCGCCATTGGATCTGCCGGGGCAATTGGCCTAATGGGGTATCAAGGGAATACACGTGTGCAGTGGAACAAGGTCTGCAACGTTTTCGGAAAATTCTGCCACCAAGTCGCAGCCTCCACCGCGCTGTCCTTCCTTGGATCTTTGGCATTCTTCTTCCTAGTTGTGCTTGCTGTTCTAGGCCTTAG

Protein Analysis

191

Amino Acids

20.81

Weight (kDa)

9.88

Isoelectric Point (pI)

49.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 29 - 110 3.5e-15 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15620 AT4G15630
fragaria_vesca FvH4_4g17290
malus_domestica MD13G1173100.v1.1
prunus_persica Prupe.1G141500_v2.0.a1
pyrus_communis pycom13g14870 pycom16g14610
rosa_chinensis RchiOBHm_Chr4g0421251
rosa_laevigata RLG00000007706
rosa_multiflora Rmu_sc0009290.1_g000018
rosa_roxburghii Rroxscaffold_5G00363490
rosa_rugosa Rorug04G0169700
rosa_samantha Rh4AG229900 Rh4BG232600 Rh4CG244400 Rh4DG228600
rosa_wichuraiana Rw4G020370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 88
AccII CGCG 1 cut(s) 510
AciI CCGC 2 cut(s) 391, 508
AclI AACGTT 1 cut(s) 469
AclWI GGATC 2 cut(s) 407, 533
AcsI RAATTY 1 cut(s) 480
AcvI CACGTG 2 cut(s) 86, 444
AfaI GTAC 2 cut(s) 14, 251
AflIII ACRYGT 3 cut(s) 83, 441, 443
AhlI ACTAGT 1 cut(s) 26
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 1 cut(s) 181
AluI AGCT 1 cut(s) 181
Alw21I GWGCWC 2 cut(s) 26, 361
AlwI GGATC 2 cut(s) 407, 533
AoxI GGCC 3 cut(s) 108, 416, 566
ApeKI GCWGC 2 cut(s) 287, 498
ApoI RAATTY 1 cut(s) 480
AspLEI GCGC 1 cut(s) 512
AsuC2I CCSGG 1 cut(s) 407
AsuHPI GGTGA 1 cut(s) 358
BaeGI GKGCMC 1 cut(s) 326
BbrPI CACGTG 2 cut(s) 86, 444
Bbv12I GWGCWC 2 cut(s) 26, 361
BbvI GCAGC 2 cut(s) 299, 510
BccI CCATC 1 cut(s) 28
BceAI ACGGC 2 cut(s) 144, 234
BcgI CGANNNNNNTGC 2 cut(s) 455, 489
BciT130I CCWGG 1 cut(s) 143
BcnI CCSGG 1 cut(s) 407
BcuI ACTAGT 1 cut(s) 26
BfaI CTAG 4 cut(s) 27, 299, 546, 564
BisI GCNGC 2 cut(s) 288, 499
BlsI GCNGC 2 cut(s) 289, 500
Bme1390I CCNGG 2 cut(s) 143, 407
BmrFI CCNGG 2 cut(s) 143, 407
BmsI GCATC 1 cut(s) 256
BpuMI CCSGG 1 cut(s) 407
Bsa29I ATCGAT 2 cut(s) 173, 351
BsaAI YACGTR 2 cut(s) 86, 444
BsaJI CCNNGG 4 cut(s) 111, 142, 406, 521
BsaXI ACNNNNNCTCC 5 cut(s) 13, 26, 43, 181, 211
Bse1I ACTGG 1 cut(s) 10
BseBI CCWGG 1 cut(s) 143
BseCI ATCGAT 2 cut(s) 173, 351
BseDI CCNNGG 4 cut(s) 111, 142, 406, 521
BseGI GGATG 2 cut(s) 39, 183
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 1 cut(s) 10
BseRI GAGGAG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 326
BseXI GCAGC 2 cut(s) 299, 510
BsgI GTGCAG 1 cut(s) 468
Bsh1236I CGCG 1 cut(s) 510
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 3 cut(s) 110, 418, 568
BshVI ATCGAT 2 cut(s) 173, 351
BsiEI CGRYCG 1 cut(s) 355
BsiHKAI GWGCWC 2 cut(s) 26, 361
BsiSI CCGG 1 cut(s) 406
BslFI GGGAC 1 cut(s) 94
BsmFI GGGAC 1 cut(s) 94
BsmI GAATGC 1 cut(s) 534
BsnI GGCC 3 cut(s) 110, 418, 568
Bsp1286I GDGCHC 3 cut(s) 26, 326, 361
Bsp143I GATC 5 cut(s) 348, 352, 362, 399, 525
BspACI CCGC 2 cut(s) 391, 508
BspANI GGCC 3 cut(s) 110, 418, 568
BspCNI CTCAG 1 cut(s) 90
BspDI ATCGAT 2 cut(s) 173, 351
BspFNI CGCG 1 cut(s) 510
BspPI GGATC 2 cut(s) 407, 533
BsrI ACTGG 1 cut(s) 10
BssECI CCNNGG 4 cut(s) 111, 142, 406, 521
BssMI GATC 5 cut(s) 348, 352, 362, 399, 525
BssT1I CCWWGG 2 cut(s) 111, 521
Bst2UI CCWGG 1 cut(s) 143
BstBAI YACGTR 2 cut(s) 86, 444
BstC8I GCNNGC 2 cut(s) 277, 556
BstDEI CTNAG 3 cut(s) 98, 222, 570
BstF5I GGATG 2 cut(s) 39, 183
BstFNI CGCG 1 cut(s) 510
BstHHI GCGC 1 cut(s) 512
BstKTI GATC 5 cut(s) 351, 355, 365, 402, 528
BstMBI GATC 5 cut(s) 348, 352, 362, 399, 525
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 1 cut(s) 507
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 1 cut(s) 279
BstSCI CCNGG 2 cut(s) 141, 405
BstSLI GKGCMC 1 cut(s) 326
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 2 cut(s) 299, 510
BstX2I RGATCY 2 cut(s) 399, 525
BstXI CCANNNNNNTGG 1 cut(s) 17
BstYI RGATCY 2 cut(s) 399, 525
Bsu15I ATCGAT 2 cut(s) 173, 351
BsuRI GGCC 3 cut(s) 110, 418, 568
BsuTUI ATCGAT 2 cut(s) 173, 351
BtsCI GGATG 2 cut(s) 39, 183
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 1 cut(s) 456
Cac8I GCNNGC 2 cut(s) 277, 556
CfoI GCGC 1 cut(s) 512
ClaI ATCGAT 2 cut(s) 173, 351
Csp6I GTAC 2 cut(s) 13, 250
CviAII CATG 1 cut(s) 276
CviJI RGCY 9 cut(s) 9, 110, 131, 181, 221, 343, 418, 501, 568
CviKI_1 RGCY 9 cut(s) 9, 110, 131, 181, 221, 343, 418, 501, 568
CviQI GTAC 2 cut(s) 13, 250
DdeI CTNAG 3 cut(s) 98, 222, 570
DpnI GATC 5 cut(s) 350, 354, 364, 401, 527
DpnII GATC 5 cut(s) 348, 352, 362, 399, 525
DrdI GACNNNNNNGTC 1 cut(s) 88
DseDI GACNNNNNNGTC 1 cut(s) 88
EciI GGCGGA 1 cut(s) 380
Eco130I CCWWGG 2 cut(s) 111, 521
Eco147I AGGCCT 1 cut(s) 568
Eco72I CACGTG 2 cut(s) 86, 444
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 2 cut(s) 111, 521
ErhI CCWWGG 2 cut(s) 111, 521
FaeI CATG 1 cut(s) 279
FaiI YATR 4 cut(s) 272, 277, 283, 285
FaqI GGGAC 1 cut(s) 94
FatI CATG 1 cut(s) 275
FauNDI CATATG 1 cut(s) 283
Fnu4HI GCNGC 2 cut(s) 288, 499
FokI GGATG 2 cut(s) 46, 170
Fsp4HI GCNGC 2 cut(s) 288, 499
FspBI CTAG 4 cut(s) 27, 299, 546, 564
GlaI GCGC 1 cut(s) 511
GluI GCNGC 2 cut(s) 288, 499
HaeIII GGCC 3 cut(s) 110, 418, 568
HapII CCGG 1 cut(s) 406
HhaI GCGC 1 cut(s) 512
Hin1II CATG 1 cut(s) 279
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HinfI GANTC 4 cut(s) 89, 94, 147, 316
HpaII CCGG 1 cut(s) 406
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 99, 477
Hpy8I GTNNAC 1 cut(s) 250
HpyAV CCTTC 2 cut(s) 40, 527
HpyCH4IV ACGT 3 cut(s) 85, 443, 469
HpyCH4V TGCA 4 cut(s) 279, 287, 449, 466
HpyF10VI GCNNNNNNNGC 1 cut(s) 507
HpyF3I CTNAG 3 cut(s) 98, 222, 570
HpySE526I ACGT 3 cut(s) 85, 443, 469
Hsp92II CATG 1 cut(s) 279
HspAI GCGC 1 cut(s) 510
Kzo9I GATC 5 cut(s) 348, 352, 362, 399, 525
LmnI GCTCC 1 cut(s) 21
LpnPI CCDG 6 cut(s) 23, 128, 155, 225, 329, 419
Lsp1109I GCAGC 2 cut(s) 299, 510
LweI GCATC 1 cut(s) 256
MaeI CTAG 4 cut(s) 27, 299, 546, 564
MaeII ACGT 3 cut(s) 85, 443, 469
MalI GATC 5 cut(s) 350, 354, 364, 401, 527
MboI GATC 5 cut(s) 348, 352, 362, 399, 525
MboII GAAGA 4 cut(s) 67, 340, 530, 533
MfeI CAATTG 1 cut(s) 412
MflI RGATCY 2 cut(s) 399, 525
MhlI GDGCHC 3 cut(s) 26, 326, 361
MluCI AATT 3 cut(s) 165, 412, 480
MlyI GAGTC 2 cut(s) 98, 156
MnlI CCTC 5 cut(s) 65, 68, 93, 207, 512
MspI CCGG 1 cut(s) 406
MspR9I CCNGG 2 cut(s) 143, 407
MunI CAATTG 1 cut(s) 412
Mva1269I GAATGC 1 cut(s) 534
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 1 cut(s) 507
NciI CCSGG 1 cut(s) 407
NdeI CATATG 1 cut(s) 283
NdeII GATC 5 cut(s) 348, 352, 362, 399, 525
NlaIII CATG 1 cut(s) 279
NspI RCATGY 1 cut(s) 279
PaeI GCATGC 1 cut(s) 279
PceI AGGCCT 1 cut(s) 568
PctI GAATGC 1 cut(s) 534
PfeI GAWTC 2 cut(s) 94, 316
PkrI GCNGC 2 cut(s) 289, 500
Ple19I CGATCG 1 cut(s) 355
PleI GAGTC 2 cut(s) 97, 155
PmaCI CACGTG 2 cut(s) 86, 444
PmlI CACGTG 2 cut(s) 86, 444
PpsI GAGTC 2 cut(s) 97, 155
Ppu21I YACGTR 2 cut(s) 86, 444
Psp1406I AACGTT 1 cut(s) 469
Psp6I CCWGG 1 cut(s) 141
PspCI CACGTG 2 cut(s) 86, 444
PspGI CCWGG 1 cut(s) 141
PsuI RGATCY 2 cut(s) 399, 525
PvuI CGATCG 1 cut(s) 355
RsaI GTAC 2 cut(s) 14, 251
RsaNI GTAC 2 cut(s) 13, 250
SatI GCNGC 2 cut(s) 288, 499
Sau3AI GATC 5 cut(s) 348, 352, 362, 399, 525
SchI GAGTC 2 cut(s) 98, 156
ScrFI CCNGG 2 cut(s) 143, 407
SduI GDGCHC 3 cut(s) 26, 326, 361
SetI ASST 8 cut(s) 79, 88, 183, 194, 255, 446, 463, 472
SfaNI GCATC 1 cut(s) 256
SpeI ACTAGT 1 cut(s) 26
SphI GCATGC 1 cut(s) 279
Sse9I AATT 3 cut(s) 165, 412, 480
SseBI AGGCCT 1 cut(s) 568
SsiI CCGC 2 cut(s) 391, 508
SspMI CTAG 4 cut(s) 27, 299, 546, 564
StuI AGGCCT 1 cut(s) 568
StyD4I CCNGG 2 cut(s) 141, 405
StyI CCWWGG 2 cut(s) 111, 521
TaiI ACGT 3 cut(s) 88, 446, 472
TaqI TCGA 4 cut(s) 92, 173, 351, 361
TasI AATT 3 cut(s) 165, 412, 480
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 2 cut(s) 94, 316
TscAI CASTG 1 cut(s) 456
TseI GCWGC 2 cut(s) 287, 498
TspRI CASTG 1 cut(s) 456
XapI RAATTY 1 cut(s) 480
XceI RCATGY 1 cut(s) 279
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XspI CTAG 4 cut(s) 27, 299, 546, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.