Rorug04G0169700

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014303
Physical Location & Seq
Reverse (-)
29899292 .. 29900515
1224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0169700.1

Sequence Viewer

Length: 1224 bp
ATGTCACCTTCTTCCGATCTCCTCCGCCGTCCGTTCAAGAGGCCACTGCTCTCAGACCAGCAAAGGCGCAGAGATCTCTCTCTGAAGCGTCAAGAGCAAAGCCGTGATTCTCAGCTCCACGCCCGCCGCGTATCCTCCGCACTCCTCTCCCTCCCTCTAGAACCTGGAGCCTCCTCGACGGAGTACTACTCCGACTTGCACCTTGCCTCCGAGGTCGCTTCTAAGATTCTCAAAGGAGGCCTAGATGGTCGAAAGTGGTTTCAAAAGCAGCTCATGCTTCCCGAGTGGATGATCGACGTCCCTCATCGCTTGCCCCAAGACTGGTTTGTATTAGCCAGGCCAGCCGGGGAACGCTGCCTGGTTGTTTCATACGGCGGAACCACCGTCAGTAGGCGACGAAACGGCACCGTTCTTCATCGTTTCCCGTCTGCTTTACCCGATGGTTGCTGCAGGACCAAAGCACAACCTTCCTGTTCTATACTAGACTGTATATTTCACGAGATGGACCAGACTTATTATGTCATTGACATGTTGTGCTGGAGAGATTACTCTTTGTATGATTGCACGGCTGAGTTTAGGTTTTTTTGGTTAAAGTCTAAGCTAGCCGAGACCCGGGTTTGCGACCCTCCATCATATTATCATAGGTATAGATTCAGCTTGGTTCCTCGGTATACCTGTGATCTCACTGCCCTACACACAGCATATACCGGAGCGGTACCTTACGTAAAGGATGGTCTAATGTTTTATAATAAACATGCACATTATCAACCTGGCATAACACCCTTGGCCTTGGTTTGGAAGGATCAGAACTGTAGTAAATATGTTGTTGACACAAATAATGAAGGAAAGGTCCCAAGCCATCAGCAGATAGTTTTGGAGCTGCAACGTGATGGAAAAGTGACTACATCTGATAATCCTCCCGTTGTATTTGGTTGCTTAGGTTTGGACTTGATACAGAAATTGGGAACATTGCAGTCAGCATGTCTTCTTCGTTTTGCTATTGGTGACGGAGGATTGACCATTGTGGATGGGAAGCTTGAGAGGGCAGATTTACATTACCTTGGCAAGTCCAACCAGGCACGTGCATTCGCAGATAGTTACTCCAAAATTATGTTTCAAAGTATGGTTCGAGGATCTTCTCTTAAAATTGATGATCTGATAGCAGCAATTAGTTCATCCGACGATGGAGCAAACGGACCTGATGTTGAGATGATGGATGGTTAA

Protein Analysis

407

Amino Acids

45.98

Weight (kDa)

8.5

Isoelectric Point (pI)

51.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPN1_m3Gcap_bd PF21974 90 - 263 2.3e-53 Snurportin1, m3G cap-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15620 AT4G15630
fragaria_vesca FvH4_4g17290
malus_domestica MD13G1173100.v1.1
prunus_persica Prupe.1G141500_v2.0.a1
pyrus_communis pycom13g14870 pycom16g14610
rosa_chinensis RchiOBHm_Chr4g0421251
rosa_laevigata RLG00000007706
rosa_multiflora Rmu_sc0009290.1_g000018
rosa_roxburghii Rroxscaffold_5G00363490
rosa_rugosa Rorug04G0169700
rosa_samantha Rh4AG229900 Rh4BG232600 Rh4CG244400 Rh4DG228600
rosa_wichuraiana Rw4G020370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 747
AatII GACGTC 1 cut(s) 300
Acc65I GGTACC 1 cut(s) 715
AccB1I GGYRCC 2 cut(s) 404, 715
AccBSI CCGCTC 1 cut(s) 713
AccI GTMKAC 1 cut(s) 671
AccII CGCG 1 cut(s) 129
AciI CCGC 6 cut(s) 25, 124, 127, 138, 375, 713
AclWI GGATC 2 cut(s) 810, 1141
AcuI CTGAAG 1 cut(s) 104
AcvI CACGTG 1 cut(s) 1082
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 2 cut(s) 185, 717
AfiI CCNNNNNNNGG 4 cut(s) 321, 390, 612, 795
AflIII ACRYGT 1 cut(s) 528
AgsI TTSAA 3 cut(s) 37, 263, 1118
AjnI CCWGG 5 cut(s) 163, 335, 357, 769, 1074
AluBI AGCT 6 cut(s) 115, 271, 601, 657, 880, 1036
AluI AGCT 6 cut(s) 115, 271, 601, 657, 880, 1036
Alw26I GTCTC 1 cut(s) 602
AlwI GGATC 2 cut(s) 810, 1141
Ama87I CYCGRG 2 cut(s) 281, 612
AoxI GGCC 4 cut(s) 41, 238, 338, 786
ApeKI GCWGC 5 cut(s) 268, 354, 447, 880, 1163
Asp718I GGTACC 1 cut(s) 715
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 4 cut(s) 453, 505, 850, 1196
AsuC2I CCSGG 3 cut(s) 346, 613, 614
AsuHPI GGTGA 1 cut(s) 1016
AsuNHI GCTAGC 1 cut(s) 601
AvaI CYCGRG 2 cut(s) 281, 612
AvaII GGWCC 4 cut(s) 453, 505, 850, 1196
BanI GGYRCC 2 cut(s) 404, 715
BauI CACGAG 1 cut(s) 497
BbrPI CACGTG 1 cut(s) 1082
BbsI GAAGAC 1 cut(s) 977
BbvI GCAGC 5 cut(s) 280, 341, 434, 867, 1175
BceAI ACGGC 5 cut(s) 12, 87, 388, 418, 582
BciT130I CCWGG 5 cut(s) 165, 337, 359, 771, 1076
BciVI GTATCC 1 cut(s) 142
BcnI CCSGG 3 cut(s) 346, 613, 614
BcoDI GTCTC 1 cut(s) 602
BfaI CTAG 4 cut(s) 158, 242, 482, 602
BfmI CTRYAG 2 cut(s) 448, 811
BfuI GTATCC 1 cut(s) 142
BglII AGATCT 1 cut(s) 73
BisI GCNGC 6 cut(s) 127, 269, 355, 448, 881, 1164
BlsI GCNGC 6 cut(s) 128, 270, 356, 449, 882, 1165
BmcAI AGTACT 1 cut(s) 185
Bme1390I CCNGG 8 cut(s) 165, 337, 346, 359, 613, 614, 771, 1076
Bme18I GGWCC 4 cut(s) 453, 505, 850, 1196
BmeT110I CYCGRG 2 cut(s) 281, 612
BmgT120I GGNCC 4 cut(s) 453, 505, 850, 1196
BmiI GGNNCC 6 cut(s) 169, 379, 406, 663, 717, 852
BmrFI CCNGG 8 cut(s) 165, 337, 346, 359, 613, 614, 771, 1076
BmtI GCTAGC 1 cut(s) 605
BpiI GAAGAC 1 cut(s) 977
BplI GAGNNNNNCTC 2 cut(s) 173, 205
BpmI CTGGAG 2 cut(s) 186, 559
Bpu10I CCTNAGC 1 cut(s) 937
BpuEI CTTGAG 1 cut(s) 1058
BpuMI CCSGG 3 cut(s) 346, 613, 614
BsaAI YACGTR 2 cut(s) 724, 1082
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 602
BsaJI CCNNGG 7 cut(s) 210, 345, 612, 665, 783, 789, 1060
BsaWI WCCGGW 1 cut(s) 707
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 4 cut(s) 321, 390, 612, 795
Bse1I ACTGG 1 cut(s) 326
Bse3DI GCAATG 1 cut(s) 968
BseBI CCWGG 5 cut(s) 165, 337, 359, 771, 1076
BseDI CCNNGG 7 cut(s) 210, 345, 612, 665, 783, 789, 1060
BseGI GGATG 5 cut(s) 294, 736, 1033, 1175, 1222
BseLI CCNNNNNNNGG 4 cut(s) 321, 390, 612, 795
BseMI GCAATG 1 cut(s) 968
BseMII CTCAG 3 cut(s) 66, 125, 561
BseNI ACTGG 1 cut(s) 326
BseRI GAGGAG 3 cut(s) 11, 134, 163
BseXI GCAGC 5 cut(s) 280, 341, 434, 867, 1175
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 4 cut(s) 43, 240, 340, 788
BshNI GGYRCC 2 cut(s) 404, 715
BsiHKCI CYCGRG 2 cut(s) 281, 612
BsiSI CCGG 3 cut(s) 345, 613, 708
BslFI GGGAC 2 cut(s) 284, 836
BslI CCNNNNNNNGG 4 cut(s) 321, 390, 612, 795
BsmAI GTCTC 1 cut(s) 602
BsmFI GGGAC 2 cut(s) 284, 836
BsmI GAATGC 1 cut(s) 1085
BsnI GGCC 4 cut(s) 43, 240, 340, 788
Bso31I GGTCTC 1 cut(s) 602
BsoBI CYCGRG 2 cut(s) 281, 612
Bsp143I GATC 7 cut(s) 16, 73, 291, 679, 802, 1133, 1153
BspACI CCGC 6 cut(s) 25, 124, 127, 138, 375, 713
BspANI GGCC 4 cut(s) 43, 240, 340, 788
BspCNI CTCAG 3 cut(s) 65, 124, 562
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 6 cut(s) 169, 379, 406, 663, 717, 852
BspMAI CTGCAG 1 cut(s) 452
BspOI GCTAGC 1 cut(s) 605
BspPI GGATC 2 cut(s) 810, 1141
BspT107I GGYRCC 2 cut(s) 404, 715
BspTNI GGTCTC 1 cut(s) 602
BsrBI CCGCTC 1 cut(s) 713
BsrDI GCAATG 1 cut(s) 968
BsrI ACTGG 1 cut(s) 326
BssECI CCNNGG 7 cut(s) 210, 345, 612, 665, 783, 789, 1060
BssMI GATC 7 cut(s) 16, 73, 291, 679, 802, 1133, 1153
BssNAI GTATAC 1 cut(s) 672
BssNI GRCGYC 1 cut(s) 297
BssSI CACGAG 1 cut(s) 497
BssT1I CCWWGG 3 cut(s) 783, 789, 1060
Bst1107I GTATAC 1 cut(s) 672
Bst2BI CACGAG 1 cut(s) 497
Bst2UI CCWGG 5 cut(s) 165, 337, 359, 771, 1076
Bst4CI ACNGT 4 cut(s) 385, 409, 488, 812
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 274
BstBAI YACGTR 2 cut(s) 724, 1082
BstC8I GCNNGC 4 cut(s) 124, 311, 342, 603
BstDEI CTNAG 6 cut(s) 52, 111, 222, 570, 597, 937
BstF5I GGATG 5 cut(s) 294, 736, 1033, 1175, 1222
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 7 cut(s) 19, 76, 294, 682, 805, 1136, 1156
BstMAI GTCTC 1 cut(s) 602
BstMBI GATC 7 cut(s) 16, 73, 291, 679, 802, 1133, 1153
BstMWI GCNNNNNNNGC 3 cut(s) 94, 274, 341
BstNI CCWGG 5 cut(s) 165, 337, 359, 771, 1076
BstNSI RCATGY 3 cut(s) 532, 758, 984
BstSCI CCNGG 8 cut(s) 163, 335, 344, 357, 611, 612, 769, 1074
BstSFI CTRYAG 2 cut(s) 448, 811
BstSNI TACGTA 1 cut(s) 724
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 5 cut(s) 280, 341, 434, 867, 1175
BstV2I GAAGAC 1 cut(s) 977
BstX2I RGATCY 2 cut(s) 73, 1133
BstYI RGATCY 2 cut(s) 73, 1133
BstZ17I GTATAC 1 cut(s) 672
BsuI GTATCC 1 cut(s) 142
BsuRI GGCC 4 cut(s) 43, 240, 340, 788
BtgZI GCGATG 1 cut(s) 290
BtsCI GGATG 5 cut(s) 294, 736, 1033, 1175, 1222
BtsI GCAGTG 2 cut(s) 44, 684
BtsIMutI CAGTG 2 cut(s) 44, 684
Cac8I GCNNGC 4 cut(s) 124, 311, 342, 603
CfoI GCGC 1 cut(s) 69
Cfr13I GGNCC 4 cut(s) 453, 505, 850, 1196
Cfr9I CCCGGG 1 cut(s) 612
CseI GACGC 1 cut(s) 77
Csp6I GTAC 2 cut(s) 184, 716
CviAII CATG 4 cut(s) 274, 529, 755, 981
CviQI GTAC 2 cut(s) 184, 716
DdeI CTNAG 6 cut(s) 52, 111, 222, 570, 597, 937
DpnI GATC 7 cut(s) 18, 75, 293, 681, 804, 1135, 1155
DpnII GATC 7 cut(s) 16, 73, 291, 679, 802, 1133, 1153
EciI GGCGGA 2 cut(s) 14, 390
Eco105I TACGTA 1 cut(s) 724
Eco130I CCWWGG 3 cut(s) 783, 789, 1060
Eco147I AGGCCT 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 602
Eco47I GGWCC 4 cut(s) 453, 505, 850, 1196
Eco57I CTGAAG 1 cut(s) 104
Eco72I CACGTG 1 cut(s) 1082
Eco88I CYCGRG 2 cut(s) 281, 612
EcoO109I RGGNCCY 1 cut(s) 850
EcoRII CCWGG 5 cut(s) 163, 335, 357, 769, 1074
EcoT14I CCWWGG 3 cut(s) 783, 789, 1060
ErhI CCWWGG 3 cut(s) 783, 789, 1060
FaeI CATG 4 cut(s) 277, 532, 758, 984
FaqI GGGAC 2 cut(s) 284, 836
FatI CATG 4 cut(s) 273, 528, 754, 980
FauI CCCGC 1 cut(s) 131
FblI GTMKAC 1 cut(s) 671
Fnu4HI GCNGC 6 cut(s) 127, 269, 355, 448, 881, 1164
FokI GGATG 4 cut(s) 301, 743, 1040, 1162
Fsp4HI GCNGC 6 cut(s) 127, 269, 355, 448, 881, 1164
FspBI CTAG 4 cut(s) 158, 242, 482, 602
GlaI GCGC 1 cut(s) 68
GluI GCNGC 6 cut(s) 127, 269, 355, 448, 881, 1164
GsuI CTGGAG 2 cut(s) 186, 559
HaeIII GGCC 4 cut(s) 43, 240, 340, 788
HapII CCGG 3 cut(s) 345, 613, 708
HgaI GACGC 1 cut(s) 77
HhaI GCGC 1 cut(s) 69
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 4 cut(s) 277, 532, 758, 984
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HincII GTYRAC 1 cut(s) 829
HindII GTYRAC 1 cut(s) 829
HindIII AAGCTT 1 cut(s) 1034
HinfI GANTC 3 cut(s) 107, 226, 651
HpaII CCGG 3 cut(s) 345, 613, 708
HphI GGTGA 1 cut(s) 1016
Hpy166II GTNNAC 2 cut(s) 672, 829
Hpy188I TCNGA 9 cut(s) 16, 55, 84, 193, 211, 807, 910, 1158, 1180
Hpy188III TCNNGA 5 cut(s) 37, 92, 158, 281, 497
Hpy8I GTNNAC 2 cut(s) 672, 829
Hpy99I CGWCG 4 cut(s) 181, 299, 399, 1184
HpyAV CCTTC 4 cut(s) 18, 477, 793, 836
HpyCH4III ACNGT 4 cut(s) 385, 409, 488, 812
HpyCH4IV ACGT 4 cut(s) 297, 723, 886, 1081
HpyCH4V TGCA 7 cut(s) 199, 450, 564, 758, 883, 973, 1085
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 274, 341
HpyF3I CTNAG 6 cut(s) 52, 111, 222, 570, 597, 937
HpySE526I ACGT 4 cut(s) 297, 723, 886, 1081
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 4 cut(s) 277, 532, 758, 984
HspAI GCGC 1 cut(s) 67
KpnI GGTACC 1 cut(s) 719
Kzo9I GATC 7 cut(s) 16, 73, 291, 679, 802, 1133, 1153
LmnI GCTCC 5 cut(s) 120, 167, 710, 877, 1187
Lsp1109I GCAGC 5 cut(s) 280, 341, 434, 867, 1175
MaeI CTAG 4 cut(s) 158, 242, 482, 602
MaeII ACGT 4 cut(s) 297, 723, 886, 1081
MaeIII GTNAC 4 cut(s) 3, 898, 1004, 1097
MalI GATC 7 cut(s) 18, 75, 293, 681, 804, 1135, 1155
MbiI CCGCTC 1 cut(s) 713
MboI GATC 7 cut(s) 16, 73, 291, 679, 802, 1133, 1153
MboII GAAGA 5 cut(s) 3, 404, 977, 980, 1128
MflI RGATCY 2 cut(s) 73, 1133
MluCI AATT 4 cut(s) 959, 1107, 1146, 1167
MmeI TCCRAC 3 cut(s) 216, 1095, 1203
MseI TTAA 3 cut(s) 590, 1143, 1222
MslI CAYNNNNRTG 1 cut(s) 527
MspI CCGG 3 cut(s) 345, 613, 708
MspR9I CCNGG 8 cut(s) 165, 337, 346, 359, 613, 614, 771, 1076
Mva1269I GAATGC 1 cut(s) 1085
MvaI CCWGG 5 cut(s) 165, 337, 359, 771, 1076
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 94, 274, 341
NciI CCSGG 3 cut(s) 346, 613, 614
NdeII GATC 7 cut(s) 16, 73, 291, 679, 802, 1133, 1153
NheI GCTAGC 1 cut(s) 601
NlaIII CATG 4 cut(s) 277, 532, 758, 984
NlaIV GGNNCC 6 cut(s) 169, 379, 406, 663, 717, 852
NmeAIII GCCGAG 1 cut(s) 631
NmuCI GTSAC 3 cut(s) 3, 898, 1004
NspI RCATGY 3 cut(s) 532, 758, 984
PceI AGGCCT 1 cut(s) 240
PciI ACATGT 1 cut(s) 528
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 1085
PfeI GAWTC 3 cut(s) 107, 226, 651
PkrI GCNGC 6 cut(s) 128, 270, 356, 449, 882, 1165
PmaCI CACGTG 1 cut(s) 1082
PmlI CACGTG 1 cut(s) 1082
Ppu21I YACGTR 2 cut(s) 724, 1082
PpuMI RGGWCCY 1 cut(s) 850
PscI ACATGT 1 cut(s) 528
PsiI TTATAA 1 cut(s) 747
Psp5II RGGWCCY 1 cut(s) 850
Psp6I CCWGG 5 cut(s) 163, 335, 357, 769, 1074
PspCI CACGTG 1 cut(s) 1082
PspGI CCWGG 5 cut(s) 163, 335, 357, 769, 1074
PspN4I GGNNCC 6 cut(s) 169, 379, 406, 663, 717, 852
PspPI GGNCC 4 cut(s) 453, 505, 850, 1196
PspPPI RGGWCCY 1 cut(s) 850
PstI CTGCAG 1 cut(s) 452
PsuI RGATCY 2 cut(s) 73, 1133
RsaI GTAC 2 cut(s) 185, 717
RsaNI GTAC 2 cut(s) 184, 716
RseI CAYNNNNRTG 1 cut(s) 527
SaqAI TTAA 3 cut(s) 590, 1143, 1222
SatI GCNGC 6 cut(s) 127, 269, 355, 448, 881, 1164
Sau3AI GATC 7 cut(s) 16, 73, 291, 679, 802, 1133, 1153
Sau96I GGNCC 4 cut(s) 453, 505, 850, 1196
ScaI AGTACT 1 cut(s) 185
ScrFI CCNGG 8 cut(s) 165, 337, 346, 359, 613, 614, 771, 1076
SfcI CTRYAG 2 cut(s) 448, 811
SinI GGWCC 4 cut(s) 453, 505, 850, 1196
SmaI CCCGGG 1 cut(s) 614
SmiMI CAYNNNNRTG 1 cut(s) 527
SmlI CTYRAG 1 cut(s) 1037
SmoI CTYRAG 1 cut(s) 1037
SnaBI TACGTA 1 cut(s) 724
Sse9I AATT 4 cut(s) 959, 1107, 1146, 1167
SseBI AGGCCT 1 cut(s) 240
SsiI CCGC 6 cut(s) 25, 124, 127, 138, 375, 713
SspMI CTAG 4 cut(s) 158, 242, 482, 602
StuI AGGCCT 1 cut(s) 240
StyD4I CCNGG 8 cut(s) 163, 335, 344, 357, 611, 612, 769, 1074
StyI CCWWGG 3 cut(s) 783, 789, 1060
TaaI ACNGT 4 cut(s) 385, 409, 488, 812
TaiI ACGT 4 cut(s) 300, 726, 889, 1084
TaqI TCGA 4 cut(s) 176, 250, 294, 1129
TasI AATT 4 cut(s) 959, 1107, 1146, 1167
TatI WGTACW 1 cut(s) 183
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 3 cut(s) 107, 226, 651
Tru1I TTAA 3 cut(s) 590, 1143, 1222
Tru9I TTAA 3 cut(s) 590, 1143, 1222
TscAI CASTG 2 cut(s) 51, 691
TseFI GTSAC 3 cut(s) 3, 898, 1004
TseI GCWGC 5 cut(s) 268, 354, 447, 880, 1163
Tsp45I GTSAC 3 cut(s) 3, 898, 1004
TspDTI ATGAA 4 cut(s) 357, 404, 855, 1164
TspGWI ACGGA 4 cut(s) 21, 194, 1023, 1209
TspMI CCCGGG 1 cut(s) 612
TspRI CASTG 2 cut(s) 51, 691
VpaK11BI GGWCC 4 cut(s) 453, 505, 850, 1196
XbaI TCTAGA 1 cut(s) 157
XceI RCATGY 3 cut(s) 532, 758, 984
XmaI CCCGGG 1 cut(s) 612
XmiI GTMKAC 1 cut(s) 671
XspI CTAG 4 cut(s) 158, 242, 482, 602
ZraI GACGTC 1 cut(s) 298
ZrmI AGTACT 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.