FvH4_4g17290

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014303
Physical Location & Seq
Forward (+)
21249495 .. 21250774
1280 bp
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UTR
Exon/CDS
Intron
FvH4_4g17290.t1

Sequence Viewer

Length: 606 bp
ATGGAGAGCCAGTACATAGGAGGCCATAGCAAGATCGCTGGTGGCGGGACGCCGGAGAATAAGGAAGTGAGGGTAGCAAACCAGAGAAGAGTTGGGAGTTTAGAGCCCCTTGTGAGGGTTTTGGCCGTAGTCCTAACTCTGACGGCTGCTTTAGTTCAAGCTTTGGATAAGCAGACCACAGTTGCTTCAATTAAGTTGGTTCCAACCTTGCCAGCCATTGATGTTCCAGTCACAGCCAAATGGCTACACCTATCTGCTTTTGTGTACTTGGTGGCTGCAAATATCATAGCATGCGTGTACGCAGCATTTTCCCTAGTTCTTTCCTTTGCAAACCGGGGCACAAAGAAGAGCTCACTAGAGGTATTCATCATCGTCCTTGATATGTTAATGGTGGGGCTGCTGTTCTCCGGCATCGGAGCCGCCACGGCTATTGGACTAATCGGGTACAATGGGAATTCACATGTCAAGTGGGATAAGGTCTGCTATGTGTTCGGAAGGTTTTGTCACCAGGTGATCGCAGCAGTGGTCCTTACTCAGTTAGGAGCTCTTGCATTTATCTTCCTTGTTGTGCTTGCTGTCTCACGTCTTAACAAGAGGTGTACTTAA

Protein Analysis

202

Amino Acids

21.48

Weight (kDa)

9.66

Isoelectric Point (pI)

28.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 30 - 183 1.7e-36 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15620 AT4G15630
fragaria_vesca FvH4_4g17290
malus_domestica MD13G1173100.v1.1
prunus_persica Prupe.1G141500_v2.0.a1
pyrus_communis pycom13g14870 pycom16g14610
rosa_chinensis RchiOBHm_Chr4g0421251
rosa_laevigata RLG00000007706
rosa_multiflora Rmu_sc0009290.1_g000018
rosa_roxburghii Rroxscaffold_5G00363490
rosa_rugosa Rorug04G0169700
rosa_samantha Rh4AG229900 Rh4BG232600 Rh4CG244400 Rh4DG228600
rosa_wichuraiana Rw4G020370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 45, 420
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 1 cut(s) 454
AcyI GRCGYC 1 cut(s) 50
AdeI CACNNNGTG 1 cut(s) 511
AfaI GTAC 5 cut(s) 14, 266, 299, 446, 601
AfiI CCNNNNNNNGG 2 cut(s) 114, 115
AflIII ACRYGT 1 cut(s) 460
AgsI TTSAA 2 cut(s) 158, 189
AjiI CACGTC 1 cut(s) 584
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 3 cut(s) 161, 351, 545
AluI AGCT 3 cut(s) 161, 351, 545
Alw21I GWGCWC 2 cut(s) 353, 547
Alw26I GTCTC 1 cut(s) 583
AoxI GGCC 2 cut(s) 22, 123
ApeKI GCWGC 5 cut(s) 146, 275, 302, 397, 518
ApoI RAATTY 1 cut(s) 454
AspS9I GGNCC 1 cut(s) 526
AsuC2I CCSGG 1 cut(s) 335
AsuHPI GGTGA 2 cut(s) 497, 523
AvaII GGWCC 1 cut(s) 526
BaeGI GKGCMC 1 cut(s) 341
BanII GRGCYC 3 cut(s) 108, 353, 547
Bbv12I GWGCWC 2 cut(s) 353, 547
BbvI GCAGC 5 cut(s) 133, 262, 314, 384, 530
BceAI ACGGC 3 cut(s) 110, 159, 441
BciT130I CCWGG 1 cut(s) 509
BcnI CCSGG 1 cut(s) 335
BcoDI GTCTC 1 cut(s) 583
BfaI CTAG 2 cut(s) 314, 356
BglI GCCNNNNNGGC 1 cut(s) 425
BisI GCNGC 6 cut(s) 147, 276, 303, 398, 420, 519
BlsI GCNGC 6 cut(s) 148, 277, 304, 399, 421, 520
Bme1390I CCNGG 2 cut(s) 335, 509
Bme18I GGWCC 1 cut(s) 526
BmgBI CACGTC 1 cut(s) 584
BmgT120I GGNCC 1 cut(s) 526
BmiI GGNNCC 2 cut(s) 201, 418
BmrFI CCNGG 2 cut(s) 335, 509
BmsI GCATC 1 cut(s) 420
BpuMI CCSGG 1 cut(s) 335
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 334, 423
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 115
Bse1I ACTGG 2 cut(s) 10, 227
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 2 cut(s) 334, 423
BseLI CCNNNNNNNGG 2 cut(s) 114, 115
BseMII CTCAG 1 cut(s) 548
BseNI ACTGG 2 cut(s) 10, 227
BseSI GKGCMC 1 cut(s) 341
BseXI GCAGC 5 cut(s) 133, 262, 314, 384, 530
BshFI GGCC 2 cut(s) 24, 125
BsiHKAI GWGCWC 2 cut(s) 353, 547
BsiSI CCGG 3 cut(s) 53, 334, 408
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 2 cut(s) 114, 115
BsmAI GTCTC 1 cut(s) 583
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 2 cut(s) 24, 125
Bsp1286I GDGCHC 4 cut(s) 108, 341, 353, 547
Bsp143I GATC 2 cut(s) 33, 513
BspACI CCGC 2 cut(s) 45, 420
BspANI GGCC 2 cut(s) 24, 125
BspCNI CTCAG 1 cut(s) 547
BspLI GGNNCC 2 cut(s) 201, 418
BspQI GCTCTTC 1 cut(s) 341
BsrI ACTGG 2 cut(s) 10, 227
BssECI CCNNGG 2 cut(s) 334, 423
BssMI GATC 2 cut(s) 33, 513
BssNI GRCGYC 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 2 cut(s) 82, 341
BstACI GRCGYC 1 cut(s) 50
BstC8I GCNNGC 3 cut(s) 213, 292, 573
BstDEI CTNAG 1 cut(s) 534
BstDSI CCRYGG 1 cut(s) 423
BstKTI GATC 2 cut(s) 36, 516
BstMAI GTCTC 1 cut(s) 583
BstMBI GATC 2 cut(s) 33, 513
BstMWI GCNNNNNNNGC 1 cut(s) 425
BstNI CCWGG 1 cut(s) 509
BstNSI RCATGY 2 cut(s) 294, 464
BstSCI CCNGG 2 cut(s) 333, 507
BstSLI GKGCMC 1 cut(s) 341
BstV1I GCAGC 5 cut(s) 133, 262, 314, 384, 530
BsuRI GGCC 2 cut(s) 24, 125
BtgI CCRYGG 1 cut(s) 423
BtrI CACGTC 1 cut(s) 584
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 1 cut(s) 528
Cac8I GCNNGC 3 cut(s) 213, 292, 573
Cfr13I GGNCC 1 cut(s) 526
CseI GACGC 1 cut(s) 58
CsiI ACCWGGT 1 cut(s) 507
Csp6I GTAC 5 cut(s) 13, 265, 298, 445, 600
CspCI CAANNNNNGTGG 2 cut(s) 412, 447
CviAII CATG 2 cut(s) 291, 461
CviQI GTAC 5 cut(s) 13, 265, 298, 445, 600
DdeI CTNAG 1 cut(s) 534
DpnI GATC 2 cut(s) 35, 515
DpnII GATC 2 cut(s) 33, 513
DraIII CACNNNGTG 1 cut(s) 511
EaeI YGGCCR 1 cut(s) 123
Eam1104I CTCTTC 2 cut(s) 82, 341
EarI CTCTTC 2 cut(s) 82, 341
Ecl136II GAGCTC 2 cut(s) 351, 545
Eco24I GRGCYC 3 cut(s) 108, 353, 547
Eco47I GGWCC 1 cut(s) 526
Eco53kI GAGCTC 2 cut(s) 351, 545
EcoICRI GAGCTC 2 cut(s) 351, 545
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 1 cut(s) 507
EcoT38I GRGCYC 3 cut(s) 108, 353, 547
FaeI CATG 2 cut(s) 294, 464
FaiI YATR 7 cut(s) 17, 27, 287, 292, 383, 462, 486
FaqI GGGAC 1 cut(s) 61
FatI CATG 2 cut(s) 290, 460
FauI CCCGC 1 cut(s) 38
Fnu4HI GCNGC 6 cut(s) 147, 276, 303, 398, 420, 519
FriOI GRGCYC 3 cut(s) 108, 353, 547
Fsp4HI GCNGC 6 cut(s) 147, 276, 303, 398, 420, 519
FspBI CTAG 2 cut(s) 314, 356
GluI GCNGC 6 cut(s) 147, 276, 303, 398, 420, 519
HaeIII GGCC 2 cut(s) 24, 125
HapII CCGG 3 cut(s) 53, 334, 408
HgaI GACGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 2 cut(s) 294, 464
HindIII AAGCTT 1 cut(s) 159
HpaII CCGG 3 cut(s) 53, 334, 408
HphI GGTGA 2 cut(s) 497, 523
Hpy166II GTNNAC 3 cut(s) 265, 298, 600
Hpy188I TCNGA 3 cut(s) 141, 416, 494
Hpy8I GTNNAC 3 cut(s) 265, 298, 600
HpyAV CCTTC 1 cut(s) 489
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 583
HpyCH4V TGCA 3 cut(s) 278, 329, 551
HpyF10VI GCNNNNNNNGC 1 cut(s) 425
HpyF3I CTNAG 1 cut(s) 534
HpySE526I ACGT 1 cut(s) 583
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 2 cut(s) 294, 464
Kzo9I GATC 2 cut(s) 33, 513
LguI GCTCTTC 1 cut(s) 341
LmnI GCTCC 2 cut(s) 416, 542
Lsp1109I GCAGC 5 cut(s) 133, 262, 314, 384, 530
LweI GCATC 1 cut(s) 420
MabI ACCWGGT 1 cut(s) 507
MaeI CTAG 2 cut(s) 314, 356
MaeII ACGT 1 cut(s) 583
MaeIII GTNAC 2 cut(s) 229, 503
MalI GATC 2 cut(s) 35, 515
MboI GATC 2 cut(s) 33, 513
MboII GAAGA 3 cut(s) 99, 358, 550
MhlI GDGCHC 4 cut(s) 108, 341, 353, 547
MluCI AATT 2 cut(s) 189, 454
MmeI TCCRAC 1 cut(s) 227
MnlI CCTC 5 cut(s) 14, 63, 108, 352, 588
MseI TTAA 4 cut(s) 192, 386, 588, 604
MspI CCGG 3 cut(s) 53, 334, 408
MspR9I CCNGG 2 cut(s) 335, 509
MvaI CCWGG 1 cut(s) 509
MwoI GCNNNNNNNGC 1 cut(s) 425
NciI CCSGG 1 cut(s) 335
NdeII GATC 2 cut(s) 33, 513
NlaIII CATG 2 cut(s) 294, 464
NlaIV GGNNCC 2 cut(s) 201, 418
NmuCI GTSAC 2 cut(s) 229, 503
NspI RCATGY 2 cut(s) 294, 464
PaeI GCATGC 1 cut(s) 294
PciI ACATGT 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 341
PkrI GCNGC 6 cut(s) 148, 277, 304, 399, 421, 520
PscI ACATGT 1 cut(s) 460
Psp124BI GAGCTC 2 cut(s) 353, 547
Psp6I CCWGG 1 cut(s) 507
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 2 cut(s) 201, 418
PspPI GGNCC 1 cut(s) 526
RsaI GTAC 5 cut(s) 14, 266, 299, 446, 601
RsaNI GTAC 5 cut(s) 13, 265, 298, 445, 600
SacI GAGCTC 2 cut(s) 353, 547
SapI GCTCTTC 1 cut(s) 341
SaqAI TTAA 4 cut(s) 192, 386, 588, 604
SatI GCNGC 6 cut(s) 147, 276, 303, 398, 420, 519
Sau3AI GATC 2 cut(s) 33, 513
Sau96I GGNCC 1 cut(s) 526
ScrFI CCNGG 2 cut(s) 335, 509
SduI GDGCHC 4 cut(s) 108, 341, 353, 547
SexAI ACCWGGT 1 cut(s) 507
SfaNI GCATC 1 cut(s) 420
SinI GGWCC 1 cut(s) 526
SphI GCATGC 1 cut(s) 294
Sse9I AATT 2 cut(s) 189, 454
SsiI CCGC 2 cut(s) 45, 420
SspMI CTAG 2 cut(s) 314, 356
SstI GAGCTC 2 cut(s) 353, 547
StyD4I CCNGG 2 cut(s) 333, 507
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 1 cut(s) 586
TasI AATT 2 cut(s) 189, 454
TatI WGTACW 3 cut(s) 12, 264, 599
TauI GCSGC 1 cut(s) 422
Tru1I TTAA 4 cut(s) 192, 386, 588, 604
Tru9I TTAA 4 cut(s) 192, 386, 588, 604
TscAI CASTG 1 cut(s) 528
TseFI GTSAC 2 cut(s) 229, 503
TseI GCWGC 5 cut(s) 146, 275, 302, 397, 518
Tsp45I GTSAC 2 cut(s) 229, 503
TspDTI ATGAA 1 cut(s) 355
TspRI CASTG 1 cut(s) 528
VpaK11BI GGWCC 1 cut(s) 526
XapI RAATTY 1 cut(s) 454
XceI RCATGY 2 cut(s) 294, 464
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XspI CTAG 2 cut(s) 314, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.