pycom16g14610

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014303
Physical Location & Seq
Reverse (-)
10695966 .. 10697953
1988 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g14610.1

Sequence Viewer

Length: 552 bp
ATGGAACAGCAAAAGGAAGTGAAGATAGCAAACCAAAGAGGTGGGACATGTGAGCCACTTCTGAGGATTTTGGCCTTGGTTCTAACTCTTACGGCTTCTATTGTTCTTGGAGTCAGTAAGCAAACCGAAATTGTAACGATGAAGGTCATCCCAACCTTGCCTCCTATTGATATTCCTGCTACGGCTAAGTGGCATTACTTATCTGCTTTTGTGTACCTTGTGGTAGCAGATGTCATAGCATGCGCATATGCAGCATTTTCCCTAGTCCTTTTGTTTGCGAATCGGGGAAAGAAAAATAGGTTAGGGCTGGTGATCATCATGCTTGATCTGACGATGGTGGCGTTGCTCTTCTCCGCCATTGGATCCACTGGGGCAATTGGTCTGATGGGGTACCAAGGGAATACACACGTGCAATGGAACAAGGTTTGCAATGTTTTCGGAAAATTCTGCCACCATGTCGCGGCCTCAGCCACCCTGTCCTTGCTTGGATCTCTGGCATTCTTCTTCCTTATTGTGCTTGCTGTTCTAGGCCTTAGCAGGACGTCTAACTAG

Protein Analysis

184

Amino Acids

19.69

Weight (kDa)

9.48

Isoelectric Point (pI)

24.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 14 - 165 2.2e-38 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15620 AT4G15630
fragaria_vesca FvH4_4g17290
malus_domestica MD13G1173100.v1.1
prunus_persica Prupe.1G141500_v2.0.a1
pyrus_communis pycom13g14870 pycom16g14610
rosa_chinensis RchiOBHm_Chr4g0421251
rosa_laevigata RLG00000007706
rosa_multiflora Rmu_sc0009290.1_g000018
rosa_roxburghii Rroxscaffold_5G00363490
rosa_rugosa Rorug04G0169700
rosa_samantha Rh4AG229900 Rh4BG232600 Rh4CG244400 Rh4DG228600
rosa_wichuraiana Rw4G020370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 545
Acc16I TGCGCA 1 cut(s) 244
Acc65I GGTACC 1 cut(s) 390
AccB1I GGYRCC 1 cut(s) 390
AccII CGCG 1 cut(s) 461
AciI CCGC 2 cut(s) 354, 461
AclWI GGATC 3 cut(s) 357, 370, 496
AcsI RAATTY 1 cut(s) 443
AcvI CACGTG 1 cut(s) 409
AcyI GRCGYC 1 cut(s) 542
AfaI GTAC 2 cut(s) 215, 392
AfiI CCNNNNNNNGG 1 cut(s) 460
AflIII ACRYGT 2 cut(s) 47, 406
AlwI GGATC 3 cut(s) 357, 370, 496
AoxI GGCC 3 cut(s) 72, 462, 529
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 1 cut(s) 443
Asp718I GGTACC 1 cut(s) 390
AspLEI GCGC 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 322
BamHI GGATCC 1 cut(s) 362
BanI GGYRCC 1 cut(s) 390
BbrPI CACGTG 1 cut(s) 409
BbvCI CCTCAGC 1 cut(s) 466
BbvI GCAGC 1 cut(s) 263
BccI CCATC 2 cut(s) 328, 379
BceAI ACGGC 2 cut(s) 108, 198
BcgI CGANNNNNNTGC 2 cut(s) 418, 452
BclI TGATCA 1 cut(s) 312
BfaI CTAG 3 cut(s) 263, 527, 550
BisI GCNGC 2 cut(s) 252, 462
BlsI GCNGC 2 cut(s) 253, 463
BmiI GGNNCC 2 cut(s) 364, 392
BmrI ACTGGG 1 cut(s) 378
BmuI ACTGGG 1 cut(s) 378
Bpu10I CCTNAGC 2 cut(s) 466, 533
BsaAI YACGTR 1 cut(s) 409
BsaHI GRCGYC 1 cut(s) 542
BsaJI CCNNGG 2 cut(s) 75, 394
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 373
Bse3DI GCAATG 2 cut(s) 419, 436
BseDI CCNNGG 2 cut(s) 75, 394
BseGI GGATG 1 cut(s) 147
BseLI CCNNNNNNNGG 1 cut(s) 460
BseMI GCAATG 2 cut(s) 419, 436
BseMII CTCAG 2 cut(s) 53, 480
BseNI ACTGG 1 cut(s) 373
BseXI GCAGC 1 cut(s) 263
Bsh1236I CGCG 1 cut(s) 461
BshFI GGCC 3 cut(s) 74, 464, 531
BshNI GGYRCC 1 cut(s) 390
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 1 cut(s) 460
BsmFI GGGAC 1 cut(s) 58
BsmI GAATGC 1 cut(s) 497
BsnI GGCC 3 cut(s) 74, 464, 531
Bsp143I GATC 4 cut(s) 312, 325, 362, 488
BspACI CCGC 2 cut(s) 354, 461
BspANI GGCC 3 cut(s) 74, 464, 531
BspCNI CTCAG 2 cut(s) 54, 479
BspFNI CGCG 1 cut(s) 461
BspLI GGNNCC 2 cut(s) 364, 392
BspPI GGATC 3 cut(s) 357, 370, 496
BspQI GCTCTTC 1 cut(s) 353
BspT107I GGYRCC 1 cut(s) 390
BsrDI GCAATG 2 cut(s) 419, 436
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 2 cut(s) 75, 394
BssMI GATC 4 cut(s) 312, 325, 362, 488
BssNI GRCGYC 1 cut(s) 542
BssT1I CCWWGG 2 cut(s) 75, 394
Bst6I CTCTTC 1 cut(s) 353
BstACI GRCGYC 1 cut(s) 542
BstBAI YACGTR 1 cut(s) 409
BstC8I GCNNGC 2 cut(s) 241, 519
BstDEI CTNAG 4 cut(s) 62, 186, 466, 533
BstF5I GGATG 1 cut(s) 147
BstFNI CGCG 1 cut(s) 461
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 4 cut(s) 315, 328, 365, 491
BstMBI GATC 4 cut(s) 312, 325, 362, 488
BstMWI GCNNNNNNNGC 2 cut(s) 251, 467
BstNSI RCATGY 2 cut(s) 51, 243
BstUI CGCG 1 cut(s) 461
BstV1I GCAGC 1 cut(s) 263
BstX2I RGATCY 2 cut(s) 362, 488
BstXI CCANNNNNNTGG 1 cut(s) 41
BstYI RGATCY 2 cut(s) 362, 488
BsuRI GGCC 3 cut(s) 74, 464, 531
BtsCI GGATG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 2 cut(s) 241, 519
CfoI GCGC 1 cut(s) 245
Csp6I GTAC 2 cut(s) 214, 391
CviAII CATG 4 cut(s) 48, 240, 319, 455
CviJI RGCY 8 cut(s) 55, 74, 95, 185, 307, 464, 470, 531
CviKI_1 RGCY 8 cut(s) 55, 74, 95, 185, 307, 464, 470, 531
CviQI GTAC 2 cut(s) 214, 391
DdeI CTNAG 4 cut(s) 62, 186, 466, 533
DpnI GATC 4 cut(s) 314, 327, 364, 490
DpnII GATC 4 cut(s) 312, 325, 362, 488
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 343
Eco130I CCWWGG 2 cut(s) 75, 394
Eco147I AGGCCT 1 cut(s) 531
Eco72I CACGTG 1 cut(s) 409
EcoT14I CCWWGG 2 cut(s) 75, 394
ErhI CCWWGG 2 cut(s) 75, 394
FaeI CATG 4 cut(s) 51, 243, 322, 458
FaiI YATR 7 cut(s) 49, 236, 241, 247, 249, 320, 456
FaqI GGGAC 1 cut(s) 58
FatI CATG 4 cut(s) 47, 239, 318, 454
FauNDI CATATG 1 cut(s) 247
FbaI TGATCA 1 cut(s) 312
Fnu4HI GCNGC 2 cut(s) 252, 462
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 2 cut(s) 252, 462
FspAI RTGCGCAY 1 cut(s) 244
FspBI CTAG 3 cut(s) 263, 527, 550
FspI TGCGCA 1 cut(s) 244
GlaI GCGC 1 cut(s) 244
GluI GCNGC 2 cut(s) 252, 462
HaeIII GGCC 3 cut(s) 74, 464, 531
HhaI GCGC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 542
Hin1II CATG 4 cut(s) 51, 243, 322, 458
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HinfI GANTC 2 cut(s) 111, 280
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 4 cut(s) 63, 330, 384, 440
Hpy8I GTNNAC 1 cut(s) 214
HpyAV CCTTC 1 cut(s) 136
HpyCH4IV ACGT 2 cut(s) 408, 542
HpyCH4V TGCA 3 cut(s) 251, 412, 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 467
HpyF3I CTNAG 4 cut(s) 62, 186, 466, 533
HpySE526I ACGT 2 cut(s) 408, 542
Hsp92I GRCGYC 1 cut(s) 542
Hsp92II CATG 4 cut(s) 51, 243, 322, 458
HspAI GCGC 1 cut(s) 243
KpnI GGTACC 1 cut(s) 394
Ksp22I TGATCA 1 cut(s) 312
Kzo9I GATC 4 cut(s) 312, 325, 362, 488
LguI GCTCTTC 1 cut(s) 353
LpnPI CCDG 6 cut(s) 189, 293, 354, 479, 488, 523
Lsp1109I GCAGC 1 cut(s) 263
MaeI CTAG 3 cut(s) 263, 527, 550
MaeII ACGT 2 cut(s) 408, 542
MaeIII GTNAC 1 cut(s) 133
MalI GATC 4 cut(s) 314, 327, 364, 490
MboI GATC 4 cut(s) 312, 325, 362, 488
MboII GAAGA 4 cut(s) 34, 340, 493, 496
MfeI CAATTG 1 cut(s) 375
MflI RGATCY 2 cut(s) 362, 488
MluCI AATT 3 cut(s) 129, 375, 443
MlyI GAGTC 1 cut(s) 120
MnlI CCTC 4 cut(s) 32, 57, 171, 475
MunI CAATTG 1 cut(s) 375
Mva1269I GAATGC 1 cut(s) 497
MvnI CGCG 1 cut(s) 461
MwoI GCNNNNNNNGC 2 cut(s) 251, 467
NdeI CATATG 1 cut(s) 247
NdeII GATC 4 cut(s) 312, 325, 362, 488
NlaIII CATG 4 cut(s) 51, 243, 322, 458
NlaIV GGNNCC 2 cut(s) 364, 392
NsbI TGCGCA 1 cut(s) 244
NspI RCATGY 2 cut(s) 51, 243
PaeI GCATGC 1 cut(s) 243
PceI AGGCCT 1 cut(s) 531
PciI ACATGT 1 cut(s) 47
PciSI GCTCTTC 1 cut(s) 353
PcsI WCGNNNNNNNCGW 1 cut(s) 338
PctI GAATGC 1 cut(s) 497
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 2 cut(s) 253, 463
PleI GAGTC 1 cut(s) 119
PmaCI CACGTG 1 cut(s) 409
PmlI CACGTG 1 cut(s) 409
PpsI GAGTC 1 cut(s) 119
Ppu21I YACGTR 1 cut(s) 409
PscI ACATGT 1 cut(s) 47
PspCI CACGTG 1 cut(s) 409
PspN4I GGNNCC 2 cut(s) 364, 392
PsuI RGATCY 2 cut(s) 362, 488
RsaI GTAC 2 cut(s) 215, 392
RsaNI GTAC 2 cut(s) 214, 391
SapI GCTCTTC 1 cut(s) 353
SatI GCNGC 2 cut(s) 252, 462
Sau3AI GATC 4 cut(s) 312, 325, 362, 488
SchI GAGTC 1 cut(s) 120
SetI ASST 8 cut(s) 43, 147, 158, 219, 302, 411, 426, 545
SphI GCATGC 1 cut(s) 243
Sse9I AATT 3 cut(s) 129, 375, 443
SseBI AGGCCT 1 cut(s) 531
SsiI CCGC 2 cut(s) 354, 461
SspMI CTAG 3 cut(s) 263, 527, 550
StuI AGGCCT 1 cut(s) 531
StyI CCWWGG 2 cut(s) 75, 394
TaiI ACGT 2 cut(s) 411, 545
TasI AATT 3 cut(s) 129, 375, 443
TauI GCSGC 1 cut(s) 464
TfiI GAWTC 1 cut(s) 280
TscAI CASTG 1 cut(s) 373
TseI GCWGC 1 cut(s) 251
TspDTI ATGAA 1 cut(s) 155
TspRI CASTG 1 cut(s) 373
XapI RAATTY 1 cut(s) 443
XceI RCATGY 2 cut(s) 51, 243
XspI CTAG 3 cut(s) 263, 527, 550
ZraI GACGTC 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.