MD13G1173100.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014303
Physical Location & Seq
Reverse (-)
14204491 .. 14207227
2737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1173100.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGAGAGCCAGTACATTGGGAGCAATAGTGGGATGGAACAGCAGAAGGAAGTGAAGATAGCAAACCAAAGAGGAGGTGGGACACGTGAGTCGATTCTGAGGATTTTGGCCTTGGTGCTAACTCTTACGGCTTCTATTGTCCTTGGAGTCGGTAAGCAAACCGAAATTGTATCGTTGAAGCTCATCCCAACCTTGCCTCCTATTGATGTTCCTGCTACGGCTAAGTGGCATTACTTATCTGCTTTTGTGTACCTTGTGGCAGCAGACGCCATAGCATGCGCATATGCAGCATTTTCCCTAGTCCTTTCTTTTGCGAATCGGGGCACGAAGAATAAGTTAGGGCTGGTGATCATCGTGCTCGATCTGTCAATGGTGGCGTTGCTCTTTTCCGCCATTGGATCTGCCGGGGCAATTGGCCTAATGGGGTATCAAGGGAATACACGTGTGCAGTGGAACAAGGTCTGCAACGTTTTCGGAAAATTCTGCCACCTAGTCGCAGCCTCCACCGCCCTGTCCTTCCTTGGATCTCTGGCATTCTTCTTCCTAGTTGTGCTTGCTGTTCTAGGCCTTAGCAGGAGGTCTATGTAG

Protein Analysis

196

Amino Acids

20.76

Weight (kDa)

9.67

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 29 - 177 9.6e-38 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15620 AT4G15630
fragaria_vesca FvH4_4g17290
malus_domestica MD13G1173100.v1.1
prunus_persica Prupe.1G141500_v2.0.a1
pyrus_communis pycom13g14870 pycom16g14610
rosa_chinensis RchiOBHm_Chr4g0421251
rosa_laevigata RLG00000007706
rosa_multiflora Rmu_sc0009290.1_g000018
rosa_roxburghii Rroxscaffold_5G00363490
rosa_rugosa Rorug04G0169700
rosa_samantha Rh4AG229900 Rh4BG232600 Rh4CG244400 Rh4DG228600
rosa_wichuraiana Rw4G020370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 88
Acc16I TGCGCA 1 cut(s) 280
AciI CCGC 2 cut(s) 390, 507
AclI AACGTT 1 cut(s) 468
AclWI GGATC 2 cut(s) 406, 532
AcsI RAATTY 1 cut(s) 479
AcvI CACGTG 2 cut(s) 86, 443
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 2 cut(s) 14, 251
AflIII ACRYGT 3 cut(s) 83, 440, 442
AgsI TTSAA 1 cut(s) 178
AluBI AGCT 1 cut(s) 181
AluI AGCT 1 cut(s) 181
Alw21I GWGCWC 1 cut(s) 360
AlwI GGATC 2 cut(s) 406, 532
AoxI GGCC 3 cut(s) 108, 415, 565
ApeKI GCWGC 3 cut(s) 260, 287, 497
ApoI RAATTY 1 cut(s) 479
AspLEI GCGC 1 cut(s) 281
AsuC2I CCSGG 1 cut(s) 406
AsuHPI GGTGA 1 cut(s) 358
BaeGI GKGCMC 1 cut(s) 326
BbrPI CACGTG 2 cut(s) 86, 443
Bbv12I GWGCWC 1 cut(s) 360
BbvI GCAGC 3 cut(s) 272, 299, 509
BccI CCATC 1 cut(s) 28
BceAI ACGGC 2 cut(s) 144, 234
BcgI CGANNNNNNTGC 2 cut(s) 454, 488
BclI TGATCA 1 cut(s) 348
BcnI CCSGG 1 cut(s) 406
BfaI CTAG 4 cut(s) 299, 491, 545, 563
BisI GCNGC 3 cut(s) 261, 288, 498
BlsI GCNGC 3 cut(s) 262, 289, 499
Bme1390I CCNGG 1 cut(s) 406
BmrFI CCNGG 1 cut(s) 406
Bpu10I CCTNAGC 1 cut(s) 569
BpuMI CCSGG 1 cut(s) 406
BsaAI YACGTR 2 cut(s) 86, 443
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 111, 142, 405, 520
BsaXI ACNNNNNCTCC 5 cut(s) 13, 26, 43, 181, 211
Bse1I ACTGG 1 cut(s) 10
BseDI CCNNGG 4 cut(s) 111, 142, 405, 520
BseGI GGATG 2 cut(s) 39, 183
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 1 cut(s) 10
BseRI GAGGAG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 326
BseXI GCAGC 3 cut(s) 272, 299, 509
BsgI GTGCAG 1 cut(s) 467
BshFI GGCC 3 cut(s) 110, 417, 567
BsiHKAI GWGCWC 1 cut(s) 360
BsiSI CCGG 1 cut(s) 405
BslFI GGGAC 1 cut(s) 94
BsmFI GGGAC 1 cut(s) 94
BsmI GAATGC 1 cut(s) 533
BsnI GGCC 3 cut(s) 110, 417, 567
Bsp1286I GDGCHC 2 cut(s) 326, 360
Bsp143I GATC 4 cut(s) 348, 361, 398, 524
BspACI CCGC 2 cut(s) 390, 507
BspANI GGCC 3 cut(s) 110, 417, 567
BspCNI CTCAG 1 cut(s) 90
BspPI GGATC 2 cut(s) 406, 532
BsrI ACTGG 1 cut(s) 10
BssECI CCNNGG 4 cut(s) 111, 142, 405, 520
BssMI GATC 4 cut(s) 348, 361, 398, 524
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 3 cut(s) 111, 142, 520
BstACI GRCGYC 1 cut(s) 267
BstBAI YACGTR 2 cut(s) 86, 443
BstC8I GCNNGC 2 cut(s) 277, 555
BstDEI CTNAG 3 cut(s) 98, 222, 569
BstF5I GGATG 2 cut(s) 39, 183
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 4 cut(s) 351, 364, 401, 527
BstMBI GATC 4 cut(s) 348, 361, 398, 524
BstMWI GCNNNNNNNGC 3 cut(s) 266, 287, 506
BstNSI RCATGY 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 404
BstSLI GKGCMC 1 cut(s) 326
BstV1I GCAGC 3 cut(s) 272, 299, 509
BstX2I RGATCY 2 cut(s) 398, 524
BstXI CCANNNNNNTGG 1 cut(s) 17
BstYI RGATCY 2 cut(s) 398, 524
BsuRI GGCC 3 cut(s) 110, 417, 567
BtsCI GGATG 2 cut(s) 39, 183
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 1 cut(s) 455
Cac8I GCNNGC 2 cut(s) 277, 555
CfoI GCGC 1 cut(s) 281
CseI GACGC 1 cut(s) 275
Csp6I GTAC 2 cut(s) 13, 250
CviAII CATG 1 cut(s) 276
CviJI RGCY 9 cut(s) 9, 110, 131, 181, 221, 343, 417, 500, 567
CviKI_1 RGCY 9 cut(s) 9, 110, 131, 181, 221, 343, 417, 500, 567
CviQI GTAC 2 cut(s) 13, 250
DdeI CTNAG 3 cut(s) 98, 222, 569
DpnI GATC 4 cut(s) 350, 363, 400, 526
DpnII GATC 4 cut(s) 348, 361, 398, 524
DrdI GACNNNNNNGTC 1 cut(s) 88
DseDI GACNNNNNNGTC 1 cut(s) 88
EciI GGCGGA 1 cut(s) 379
Eco130I CCWWGG 3 cut(s) 111, 142, 520
Eco147I AGGCCT 1 cut(s) 567
Eco72I CACGTG 2 cut(s) 86, 443
EcoT14I CCWWGG 3 cut(s) 111, 142, 520
ErhI CCWWGG 3 cut(s) 111, 142, 520
FaeI CATG 1 cut(s) 279
FaiI YATR 5 cut(s) 272, 277, 283, 285, 584
FaqI GGGAC 1 cut(s) 94
FatI CATG 1 cut(s) 275
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 1 cut(s) 348
Fnu4HI GCNGC 3 cut(s) 261, 288, 498
FokI GGATG 2 cut(s) 46, 170
Fsp4HI GCNGC 3 cut(s) 261, 288, 498
FspAI RTGCGCAY 1 cut(s) 280
FspBI CTAG 4 cut(s) 299, 491, 545, 563
FspI TGCGCA 1 cut(s) 280
GlaI GCGC 1 cut(s) 280
GluI GCNGC 3 cut(s) 261, 288, 498
HaeIII GGCC 3 cut(s) 110, 417, 567
HapII CCGG 1 cut(s) 405
HgaI GACGC 1 cut(s) 275
HhaI GCGC 1 cut(s) 281
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 1 cut(s) 279
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HinfI GANTC 4 cut(s) 89, 94, 147, 316
HpaII CCGG 1 cut(s) 405
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 99, 476
Hpy8I GTNNAC 1 cut(s) 250
HpyAV CCTTC 2 cut(s) 40, 526
HpyCH4IV ACGT 3 cut(s) 85, 442, 468
HpyCH4V TGCA 3 cut(s) 287, 448, 465
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 287, 506
HpyF3I CTNAG 3 cut(s) 98, 222, 569
HpySE526I ACGT 3 cut(s) 85, 442, 468
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 1 cut(s) 279
HspAI GCGC 1 cut(s) 279
Ksp22I TGATCA 1 cut(s) 348
Kzo9I GATC 4 cut(s) 348, 361, 398, 524
LmnI GCTCC 1 cut(s) 21
LpnPI CCDG 7 cut(s) 23, 225, 329, 418, 515, 524, 559
Lsp1109I GCAGC 3 cut(s) 272, 299, 509
MaeI CTAG 4 cut(s) 299, 491, 545, 563
MaeII ACGT 3 cut(s) 85, 442, 468
MalI GATC 4 cut(s) 350, 363, 400, 526
MboI GATC 4 cut(s) 348, 361, 398, 524
MboII GAAGA 4 cut(s) 67, 340, 529, 532
MfeI CAATTG 1 cut(s) 411
MflI RGATCY 2 cut(s) 398, 524
MhlI GDGCHC 2 cut(s) 326, 360
MluCI AATT 3 cut(s) 165, 411, 479
MlyI GAGTC 2 cut(s) 98, 156
MnlI CCTC 6 cut(s) 65, 68, 93, 207, 511, 570
MspI CCGG 1 cut(s) 405
MspR9I CCNGG 1 cut(s) 406
MunI CAATTG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 533
MwoI GCNNNNNNNGC 3 cut(s) 266, 287, 506
NciI CCSGG 1 cut(s) 406
NdeI CATATG 1 cut(s) 283
NdeII GATC 4 cut(s) 348, 361, 398, 524
NlaIII CATG 1 cut(s) 279
NsbI TGCGCA 1 cut(s) 280
NspI RCATGY 1 cut(s) 279
PaeI GCATGC 1 cut(s) 279
PceI AGGCCT 1 cut(s) 567
PctI GAATGC 1 cut(s) 533
PfeI GAWTC 2 cut(s) 94, 316
PkrI GCNGC 3 cut(s) 262, 289, 499
PleI GAGTC 2 cut(s) 97, 155
PmaCI CACGTG 2 cut(s) 86, 443
PmlI CACGTG 2 cut(s) 86, 443
PpsI GAGTC 2 cut(s) 97, 155
Ppu21I YACGTR 2 cut(s) 86, 443
Psp1406I AACGTT 1 cut(s) 468
PspCI CACGTG 2 cut(s) 86, 443
PsuI RGATCY 2 cut(s) 398, 524
RsaI GTAC 2 cut(s) 14, 251
RsaNI GTAC 2 cut(s) 13, 250
SatI GCNGC 3 cut(s) 261, 288, 498
Sau3AI GATC 4 cut(s) 348, 361, 398, 524
SchI GAGTC 2 cut(s) 98, 156
ScrFI CCNGG 1 cut(s) 406
SduI GDGCHC 2 cut(s) 326, 360
SphI GCATGC 1 cut(s) 279
Sse9I AATT 3 cut(s) 165, 411, 479
SseBI AGGCCT 1 cut(s) 567
SsiI CCGC 2 cut(s) 390, 507
SspMI CTAG 4 cut(s) 299, 491, 545, 563
StuI AGGCCT 1 cut(s) 567
StyD4I CCNGG 1 cut(s) 404
StyI CCWWGG 3 cut(s) 111, 142, 520
TaiI ACGT 3 cut(s) 88, 445, 471
TaqI TCGA 2 cut(s) 92, 360
TasI AATT 3 cut(s) 165, 411, 479
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 2 cut(s) 94, 316
TscAI CASTG 1 cut(s) 455
TseI GCWGC 3 cut(s) 260, 287, 497
TspRI CASTG 1 cut(s) 455
XapI RAATTY 1 cut(s) 479
XceI RCATGY 1 cut(s) 279
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XspI CTAG 4 cut(s) 299, 491, 545, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.