Rw4G020370

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014303
Physical Location & Seq
Reverse (-)
44287446 .. 44289343
1898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020370.1

Sequence Viewer

Length: 606 bp
ATGGAGAGCCAGTACATAGGAGGCAAAAGCAATACCGATGGCGGTGGGATGCCGGAGAAGGAAGTGAGGGTAGCAAACCAGAGAAGAGTTGGGAGTTGTGAGTCGCTTGTGAGGCTTTTGGCCGTATTACTGACTCTGGTCGCTGCTATAGTTCAAGGGTTGAATAAGCAGACCAAAGTTGCTCCAATTAAGGTGGTTCCAACCTTGCCAGCCATTTATATTCCACTCACAGCCAAATGGCATTACTTATCTGCTTTTGCGTACTTGCTGGTTGCAAATGTCATAGCATGTGTATACGCAGCATTTTCCCTAGTCCTTTCCTTTGCAAACCGGGGCACAAAGAAGAGCTCACTAGGGCTATTCATCATTGTCCTTGATATATTGATAGTGGGGCTGCTTTTCTCAGCCATCGGAGCCGCCATGGCTATCGGTCTGATCGGGTACCATGGGAATTCACATGTGCAGTGGAACAAGGTCTGCAATGTGTTTGACAGATTTTGTCACCAGGTGATCGCATCAGTGGTCCTGACTCAGTTTGCAGCTCTTGCATTTATCTTCCTAGTTGTGCTTGCTATCTTACGCCTTAACAGAGGTCCACTTAAATAG

Protein Analysis

201

Amino Acids

21.7

Weight (kDa)

9.83

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 29 - 182 1.6e-37 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15620 AT4G15630
fragaria_vesca FvH4_4g17290
malus_domestica MD13G1173100.v1.1
prunus_persica Prupe.1G141500_v2.0.a1
pyrus_communis pycom13g14870 pycom16g14610
rosa_chinensis RchiOBHm_Chr4g0421251
rosa_laevigata RLG00000007706
rosa_multiflora Rmu_sc0009290.1_g000018
rosa_roxburghii Rroxscaffold_5G00363490
rosa_rugosa Rorug04G0169700
rosa_samantha Rh4AG229900 Rh4BG232600 Rh4CG244400 Rh4DG228600
rosa_wichuraiana Rw4G020370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 441
AccI GTMKAC 1 cut(s) 294
AciI CCGC 2 cut(s) 42, 417
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 1 cut(s) 451
AdeI CACNNNGTG 1 cut(s) 508
AfaI GTAC 3 cut(s) 14, 263, 443
AflIII ACRYGT 1 cut(s) 457
AgsI TTSAA 2 cut(s) 155, 163
AjnI CCWGG 1 cut(s) 504
AluBI AGCT 2 cut(s) 348, 542
AluI AGCT 2 cut(s) 348, 542
Alw21I GWGCWC 1 cut(s) 350
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 4 cut(s) 143, 299, 394, 539
ApoI RAATTY 1 cut(s) 451
Asp718I GGTACC 1 cut(s) 441
AspS9I GGNCC 2 cut(s) 523, 593
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 494, 520
AvaII GGWCC 2 cut(s) 523, 593
BaeGI GKGCMC 1 cut(s) 338
BanI GGYRCC 1 cut(s) 441
BanII GRGCYC 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 350
BbvI GCAGC 4 cut(s) 130, 311, 381, 551
BccI CCATC 2 cut(s) 32, 416
BceAI ACGGC 1 cut(s) 107
BciT130I CCWGG 1 cut(s) 506
BcnI CCSGG 1 cut(s) 332
BfaI CTAG 3 cut(s) 311, 353, 560
BfmI CTRYAG 1 cut(s) 147
BglI GCCNNNNNGGC 1 cut(s) 422
BisI GCNGC 5 cut(s) 144, 300, 395, 417, 540
BlsI GCNGC 5 cut(s) 145, 301, 396, 418, 541
Bme1390I CCNGG 2 cut(s) 332, 506
Bme18I GGWCC 2 cut(s) 523, 593
BmgT120I GGNCC 2 cut(s) 523, 593
BmiI GGNNCC 3 cut(s) 198, 415, 443
BmrFI CCNGG 2 cut(s) 332, 506
BmsI GCATC 2 cut(s) 39, 524
BoxI GACNNNNGTC 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 332
BsaJI CCNNGG 3 cut(s) 331, 420, 445
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 10
Bse3DI GCAATG 1 cut(s) 487
BseBI CCWGG 1 cut(s) 506
BseDI CCNNGG 3 cut(s) 331, 420, 445
BseGI GGATG 1 cut(s) 54
BseMI GCAATG 1 cut(s) 487
BseMII CTCAG 2 cut(s) 417, 545
BseNI ACTGG 1 cut(s) 10
BseSI GKGCMC 1 cut(s) 338
BseXI GCAGC 4 cut(s) 130, 311, 381, 551
BsgI GTGCAG 1 cut(s) 482
BshFI GGCC 1 cut(s) 122
BshNI GGYRCC 1 cut(s) 441
BsiHKAI GWGCWC 1 cut(s) 350
BsiSI CCGG 2 cut(s) 53, 331
BsnI GGCC 1 cut(s) 122
Bsp1286I GDGCHC 2 cut(s) 338, 350
Bsp143I GATC 2 cut(s) 435, 510
Bsp19I CCATGG 2 cut(s) 420, 445
BspACI CCGC 2 cut(s) 42, 417
BspANI GGCC 1 cut(s) 122
BspCNI CTCAG 2 cut(s) 416, 544
BspLI GGNNCC 3 cut(s) 198, 415, 443
BspQI GCTCTTC 1 cut(s) 338
BspT107I GGYRCC 1 cut(s) 441
BsrDI GCAATG 1 cut(s) 487
BsrI ACTGG 1 cut(s) 10
BssECI CCNNGG 3 cut(s) 331, 420, 445
BssMI GATC 2 cut(s) 435, 510
BssNAI GTATAC 1 cut(s) 295
BssT1I CCWWGG 2 cut(s) 420, 445
Bst1107I GTATAC 1 cut(s) 295
Bst2UI CCWGG 1 cut(s) 506
Bst6I CTCTTC 2 cut(s) 79, 338
BstAPI GCANNNNNTGC 1 cut(s) 545
BstC8I GCNNGC 2 cut(s) 210, 570
BstDEI CTNAG 2 cut(s) 403, 531
BstDSI CCRYGG 2 cut(s) 420, 445
BstF5I GGATG 1 cut(s) 54
BstKTI GATC 2 cut(s) 438, 513
BstMBI GATC 2 cut(s) 435, 510
BstMWI GCNNNNNNNGC 4 cut(s) 112, 413, 422, 545
BstNI CCWGG 1 cut(s) 506
BstNSI RCATGY 2 cut(s) 291, 461
BstPAI GACNNNNGTC 1 cut(s) 137
BstSCI CCNGG 2 cut(s) 330, 504
BstSFI CTRYAG 1 cut(s) 147
BstSLI GKGCMC 1 cut(s) 338
BstV1I GCAGC 4 cut(s) 130, 311, 381, 551
BstZ17I GTATAC 1 cut(s) 295
BsuRI GGCC 1 cut(s) 122
BtgI CCRYGG 2 cut(s) 420, 445
BtsCI GGATG 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 470
BtsIMutI CAGTG 2 cut(s) 470, 525
Cac8I GCNNGC 2 cut(s) 210, 570
Cfr13I GGNCC 2 cut(s) 523, 593
CsiI ACCWGGT 1 cut(s) 504
Csp6I GTAC 3 cut(s) 13, 262, 442
CspCI CAANNNNNGTGG 2 cut(s) 174, 209
CviAII CATG 4 cut(s) 288, 421, 446, 458
CviQI GTAC 3 cut(s) 13, 262, 442
DdeI CTNAG 2 cut(s) 403, 531
DpnI GATC 2 cut(s) 437, 512
DpnII GATC 2 cut(s) 435, 510
DraIII CACNNNGTG 1 cut(s) 508
EaeI YGGCCR 1 cut(s) 120
Eam1104I CTCTTC 2 cut(s) 79, 338
EarI CTCTTC 2 cut(s) 79, 338
Ecl136II GAGCTC 1 cut(s) 348
Eco130I CCWWGG 2 cut(s) 420, 445
Eco24I GRGCYC 1 cut(s) 350
Eco47I GGWCC 2 cut(s) 523, 593
Eco53kI GAGCTC 1 cut(s) 348
EcoICRI GAGCTC 1 cut(s) 348
EcoRI GAATTC 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 504
EcoT14I CCWWGG 2 cut(s) 420, 445
EcoT38I GRGCYC 1 cut(s) 350
ErhI CCWWGG 2 cut(s) 420, 445
FaeI CATG 4 cut(s) 291, 424, 449, 461
FatI CATG 4 cut(s) 287, 420, 445, 457
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 5 cut(s) 144, 300, 395, 417, 540
FokI GGATG 1 cut(s) 61
FriOI GRGCYC 1 cut(s) 350
Fsp4HI GCNGC 5 cut(s) 144, 300, 395, 417, 540
FspBI CTAG 3 cut(s) 311, 353, 560
GluI GCNGC 5 cut(s) 144, 300, 395, 417, 540
HaeIII GGCC 1 cut(s) 122
HapII CCGG 2 cut(s) 53, 331
Hin1II CATG 4 cut(s) 291, 424, 449, 461
HinfI GANTC 3 cut(s) 101, 133, 529
HpaII CCGG 2 cut(s) 53, 331
HphI GGTGA 2 cut(s) 494, 520
Hpy166II GTNNAC 2 cut(s) 295, 596
Hpy188I TCNGA 2 cut(s) 413, 435
Hpy188III TCNNGA 1 cut(s) 526
Hpy8I GTNNAC 2 cut(s) 295, 596
HpyAV CCTTC 1 cut(s) 52
HpyCH4V TGCA 6 cut(s) 275, 326, 463, 480, 539, 548
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 413, 422, 545
HpyF3I CTNAG 2 cut(s) 403, 531
Hsp92II CATG 4 cut(s) 291, 424, 449, 461
KpnI GGTACC 1 cut(s) 445
Kzo9I GATC 2 cut(s) 435, 510
LguI GCTCTTC 1 cut(s) 338
LmnI GCTCC 2 cut(s) 187, 413
Lsp1109I GCAGC 4 cut(s) 130, 311, 381, 551
LweI GCATC 2 cut(s) 39, 524
MabI ACCWGGT 1 cut(s) 504
MaeI CTAG 3 cut(s) 311, 353, 560
MaeIII GTNAC 1 cut(s) 500
MalI GATC 2 cut(s) 437, 512
MboI GATC 2 cut(s) 435, 510
MboII GAAGA 3 cut(s) 96, 355, 547
MhlI GDGCHC 2 cut(s) 338, 350
MluCI AATT 2 cut(s) 186, 451
MlyI GAGTC 3 cut(s) 110, 127, 523
MmeI TCCRAC 1 cut(s) 224
MnlI CCTC 4 cut(s) 14, 60, 105, 584
MseI TTAA 3 cut(s) 189, 585, 600
MspI CCGG 2 cut(s) 53, 331
MspR9I CCNGG 2 cut(s) 332, 506
MvaI CCWGG 1 cut(s) 506
MwoI GCNNNNNNNGC 4 cut(s) 112, 413, 422, 545
NciI CCSGG 1 cut(s) 332
NcoI CCATGG 2 cut(s) 420, 445
NdeII GATC 2 cut(s) 435, 510
NlaIII CATG 4 cut(s) 291, 424, 449, 461
NlaIV GGNNCC 3 cut(s) 198, 415, 443
NmuCI GTSAC 1 cut(s) 500
NspI RCATGY 2 cut(s) 291, 461
PciI ACATGT 1 cut(s) 457
PciSI GCTCTTC 1 cut(s) 338
PkrI GCNGC 5 cut(s) 145, 301, 396, 418, 541
PleI GAGTC 3 cut(s) 109, 127, 523
PpsI GAGTC 3 cut(s) 109, 127, 523
PscI ACATGT 1 cut(s) 457
PshAI GACNNNNGTC 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 504
PspGI CCWGG 1 cut(s) 504
PspN4I GGNNCC 3 cut(s) 198, 415, 443
PspPI GGNCC 2 cut(s) 523, 593
RsaI GTAC 3 cut(s) 14, 263, 443
RsaNI GTAC 3 cut(s) 13, 262, 442
SacI GAGCTC 1 cut(s) 350
SapI GCTCTTC 1 cut(s) 338
SaqAI TTAA 3 cut(s) 189, 585, 600
SatI GCNGC 5 cut(s) 144, 300, 395, 417, 540
Sau3AI GATC 2 cut(s) 435, 510
Sau96I GGNCC 2 cut(s) 523, 593
SchI GAGTC 3 cut(s) 110, 127, 523
ScrFI CCNGG 2 cut(s) 332, 506
SduI GDGCHC 2 cut(s) 338, 350
SetI ASST 7 cut(s) 195, 206, 350, 477, 510, 544, 595
SexAI ACCWGGT 1 cut(s) 504
SfaNI GCATC 2 cut(s) 39, 524
SfcI CTRYAG 1 cut(s) 147
SinI GGWCC 2 cut(s) 523, 593
Sse9I AATT 2 cut(s) 186, 451
SsiI CCGC 2 cut(s) 42, 417
SspMI CTAG 3 cut(s) 311, 353, 560
SstI GAGCTC 1 cut(s) 350
StyD4I CCNGG 2 cut(s) 330, 504
StyI CCWWGG 2 cut(s) 420, 445
TaqII GACCGA 1 cut(s) 419
TasI AATT 2 cut(s) 186, 451
TatI WGTACW 1 cut(s) 12
TauI GCSGC 1 cut(s) 419
Tru1I TTAA 3 cut(s) 189, 585, 600
Tru9I TTAA 3 cut(s) 189, 585, 600
TscAI CASTG 2 cut(s) 470, 525
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 4 cut(s) 143, 299, 394, 539
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 1 cut(s) 352
TspRI CASTG 2 cut(s) 470, 525
VpaK11BI GGWCC 2 cut(s) 523, 593
XapI RAATTY 1 cut(s) 451
XceI RCATGY 2 cut(s) 291, 461
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
XmiI GTMKAC 1 cut(s) 294
XspI CTAG 3 cut(s) 311, 353, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.