AT4G17245

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014108
Physical Location & Seq
Forward (+)
9669345 .. 9670005
661 bp
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UTR
Exon/CDS
Intron
AT4G17245.1

Sequence Viewer

Length: 501 bp
ATGCGGCTGCTGCTATCATCTGATCCCCAACCCGAACTTACGTCTACATGTACCTCTCATAGTTGCGGCTGGAAGCCTTACTCTCATTCCAATGACTTTGCTGCCAACGCGTTCCTCCTTCTCATCATCCTCTTCTGTTCTTTCATATGCGTCCTCTCTCTCCACGCTGCTATCCGTTGTTGCCTCCGTCCAGTCCTCCAACATGTCCCTAAGCCCGACCCGGACCTTGAGGCCACCCATCCAGACGCTCCCCCGACACTTGTCTACTCCCCGGGCCTAAACCTAGCAGGCAATGAAGCAGAGTGCATCATTTGTTTGTCTGAATTCCAAGACGGTGACACCTTACGCGTGTTGGAGAGATGCAAACATGGATTCCACGTTTATTGCATCCAGAAGTGGCTCTCCTCATCCCATTCTTCTTGTCCCACTTGCCGGACAAACATCTTCTCCTCCCCACCTCAACTTCACTCACAAAGCTTACCACTAACGAGTACACCTTAG

Protein Analysis

166

Amino Acids

18.3

Weight (kDa)

6.27

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 100 - 145 8.1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 101 - 145 3.7e-13 Ring finger domain
zf-RING_11 PF17123 101 - 130 1.6e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 101 - 144 2.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 102 - 144 7.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 116 - 147 1.2e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17245 AT5G47610
fragaria_vesca FvH4_5g10181
malus_domestica MD06G1127400.v1.1 MD10G1185300.v1.1 MD14G1142700.v1.1
prunus_persica Prupe.5G137200_v2.0.a1
pyrus_communis pycom06g11720
rosa_chinensis RchiOBHm_Chr7g0188061
rosa_laevigata RLG00000004730
rosa_multiflora Rmu_ssc0000422.1_g000018
rosa_roxburghii Rroxscaffold_3G00266770
rosa_rugosa Rorug06G0487900
rosa_samantha Rh7AG092200 Rh7BG093800 Rh7CG093100
rosa_wichuraiana Rw7G007970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 44, 264
AccII CGCG 2 cut(s) 110, 348
AciI CCGC 2 cut(s) 4, 66
AclWI GGATC 1 cut(s) 17
AcsI RAATTY 1 cut(s) 323
AfaI GTAC 2 cut(s) 52, 493
AfiI CCNNNNNNNGG 1 cut(s) 432
AflIII ACRYGT 4 cut(s) 47, 108, 202, 346
AluBI AGCT 1 cut(s) 477
AluI AGCT 1 cut(s) 477
AlwI GGATC 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 271
AoxI GGCC 2 cut(s) 231, 274
ApeKI GCWGC 4 cut(s) 7, 10, 101, 167
ApoI RAATTY 1 cut(s) 323
AspS9I GGNCC 2 cut(s) 223, 274
AsuC2I CCSGG 3 cut(s) 221, 272, 273
AsuHPI GGTGA 1 cut(s) 347
AvaI CYCGRG 1 cut(s) 271
AvaII GGWCC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 88, 154
BccI CCATC 1 cut(s) 246
BcnI CCSGG 3 cut(s) 221, 272, 273
BfaI CTAG 1 cut(s) 284
BisI GCNGC 6 cut(s) 5, 8, 11, 67, 102, 168
BlsI GCNGC 6 cut(s) 6, 9, 12, 68, 103, 169
Bme1390I CCNGG 3 cut(s) 221, 272, 273
Bme18I GGWCC 1 cut(s) 223
BmeT110I CYCGRG 1 cut(s) 271
BmgT120I GGNCC 2 cut(s) 223, 274
BmrFI CCNGG 3 cut(s) 221, 272, 273
BmsI GCATC 3 cut(s) 315, 350, 396
BoxI GACNNNNGTC 1 cut(s) 260
Bpu10I CCTNAGC 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 3 cut(s) 221, 272, 273
BsaJI CCNNGG 2 cut(s) 270, 271
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 1 cut(s) 432
Bse1I ACTGG 1 cut(s) 191
Bse3DI GCAATG 1 cut(s) 298
BseDI CCNNGG 2 cut(s) 270, 271
BseGI GGATG 4 cut(s) 126, 238, 387, 407
BseLI CCNNNNNNNGG 1 cut(s) 432
BseMI GCAATG 1 cut(s) 298
BseNI ACTGG 1 cut(s) 191
BseRI GAGGAG 2 cut(s) 394, 439
BseXI GCAGC 2 cut(s) 88, 154
Bsh1236I CGCG 2 cut(s) 110, 348
BshFI GGCC 2 cut(s) 233, 276
BsiHKCI CYCGRG 1 cut(s) 271
BsiSI CCGG 3 cut(s) 221, 272, 433
BslFI GGGAC 2 cut(s) 191, 408
BslI CCNNNNNNNGG 1 cut(s) 432
BsmFI GGGAC 2 cut(s) 191, 408
BsnI GGCC 2 cut(s) 233, 276
BsoBI CYCGRG 1 cut(s) 271
Bsp143I GATC 1 cut(s) 22
BspACI CCGC 2 cut(s) 4, 66
BspANI GGCC 2 cut(s) 233, 276
BspFNI CGCG 2 cut(s) 110, 348
BspPI GGATC 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 298
BsrI ACTGG 1 cut(s) 191
BssECI CCNNGG 2 cut(s) 270, 271
BssMI GATC 1 cut(s) 22
Bst4CI ACNGT 1 cut(s) 335
Bst6I CTCTTC 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 2 cut(s) 210, 498
BstF5I GGATG 4 cut(s) 126, 238, 387, 407
BstFNI CGCG 2 cut(s) 110, 348
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstMWI GCNNNNNNNGC 2 cut(s) 10, 107
BstNSI RCATGY 2 cut(s) 51, 206
BstPAI GACNNNNGTC 1 cut(s) 260
BstSCI CCNGG 3 cut(s) 219, 270, 271
BstUI CGCG 2 cut(s) 110, 348
BstV1I GCAGC 2 cut(s) 88, 154
BsuRI GGCC 2 cut(s) 233, 276
BtsCI GGATG 4 cut(s) 126, 238, 387, 407
Cac8I GCNNGC 1 cut(s) 289
Cfr13I GGNCC 2 cut(s) 223, 274
Cfr9I CCCGGG 1 cut(s) 271
CseI GACGC 2 cut(s) 139, 254
Csp6I GTAC 2 cut(s) 51, 492
CspCI CAANNNNNGTGG 2 cut(s) 365, 400
CviAII CATG 3 cut(s) 48, 203, 368
CviJI RGCY 8 cut(s) 7, 69, 76, 214, 233, 276, 400, 477
CviKI_1 RGCY 8 cut(s) 7, 69, 76, 214, 233, 276, 400, 477
CviQI GTAC 2 cut(s) 51, 492
DdeI CTNAG 2 cut(s) 210, 498
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
Eco47I GGWCC 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 271
EcoRI GAATTC 1 cut(s) 323
FaeI CATG 3 cut(s) 51, 206, 371
FaiI YATR 6 cut(s) 49, 60, 146, 148, 204, 369
FaqI GGGAC 2 cut(s) 191, 408
FatI CATG 3 cut(s) 47, 202, 367
FauNDI CATATG 1 cut(s) 146
FblI GTMKAC 2 cut(s) 44, 264
Fnu4HI GCNGC 6 cut(s) 5, 8, 11, 67, 102, 168
FokI GGATG 4 cut(s) 113, 225, 374, 394
Fsp4HI GCNGC 6 cut(s) 5, 8, 11, 67, 102, 168
FspBI CTAG 1 cut(s) 284
GluI GCNGC 6 cut(s) 5, 8, 11, 67, 102, 168
HaeIII GGCC 2 cut(s) 233, 276
HapII CCGG 3 cut(s) 221, 272, 433
HgaI GACGC 2 cut(s) 139, 254
Hin1II CATG 3 cut(s) 51, 206, 371
HindIII AAGCTT 1 cut(s) 475
HinfI GANTC 1 cut(s) 372
HpaII CCGG 3 cut(s) 221, 272, 433
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 3 cut(s) 45, 265, 494
Hpy188I TCNGA 2 cut(s) 22, 322
Hpy188III TCNNGA 2 cut(s) 242, 391
Hpy8I GTNNAC 3 cut(s) 45, 265, 494
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 1 cut(s) 335
HpyCH4IV ACGT 2 cut(s) 41, 378
HpyCH4V TGCA 3 cut(s) 306, 363, 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 107
HpyF3I CTNAG 2 cut(s) 210, 498
HpySE526I ACGT 2 cut(s) 41, 378
Hsp92II CATG 3 cut(s) 51, 206, 371
Kzo9I GATC 1 cut(s) 22
LmnI GCTCC 1 cut(s) 253
LpnPI CCDG 8 cut(s) 55, 204, 234, 255, 273, 285, 404, 446
Lsp1109I GCAGC 2 cut(s) 88, 154
LweI GCATC 3 cut(s) 315, 350, 396
MaeI CTAG 1 cut(s) 284
MaeII ACGT 2 cut(s) 41, 378
MaeIII GTNAC 1 cut(s) 335
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 3 cut(s) 124, 408, 436
MluCI AATT 1 cut(s) 323
MluI ACGCGT 2 cut(s) 108, 346
MmeI TCCRAC 2 cut(s) 223, 333
MslI CAYNNNNRTG 1 cut(s) 90
MspI CCGG 3 cut(s) 221, 272, 433
MspR9I CCNGG 3 cut(s) 221, 272, 273
MvnI CGCG 2 cut(s) 110, 348
MwoI GCNNNNNNNGC 2 cut(s) 10, 107
NciI CCSGG 3 cut(s) 221, 272, 273
NdeI CATATG 1 cut(s) 146
NdeII GATC 1 cut(s) 22
NlaIII CATG 3 cut(s) 51, 206, 371
NmuCI GTSAC 1 cut(s) 335
NspI RCATGY 2 cut(s) 51, 206
PciI ACATGT 2 cut(s) 47, 202
PfeI GAWTC 1 cut(s) 372
PkrI GCNGC 6 cut(s) 6, 9, 12, 68, 103, 169
PscI ACATGT 2 cut(s) 47, 202
PshAI GACNNNNGTC 1 cut(s) 260
PspPI GGNCC 2 cut(s) 223, 274
RsaI GTAC 2 cut(s) 52, 493
RsaNI GTAC 2 cut(s) 51, 492
RseI CAYNNNNRTG 1 cut(s) 90
SatI GCNGC 6 cut(s) 5, 8, 11, 67, 102, 168
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 2 cut(s) 223, 274
ScrFI CCNGG 3 cut(s) 221, 272, 273
SetI ASST 9 cut(s) 44, 56, 228, 285, 344, 381, 460, 479, 499
SfaNI GCATC 3 cut(s) 315, 350, 396
SinI GGWCC 1 cut(s) 223
SmaI CCCGGG 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 1 cut(s) 323
SsiI CCGC 2 cut(s) 4, 66
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 3 cut(s) 219, 270, 271
TaaI ACNGT 1 cut(s) 335
TaiI ACGT 2 cut(s) 44, 381
TasI AATT 1 cut(s) 323
TatI WGTACW 1 cut(s) 491
TauI GCSGC 2 cut(s) 7, 69
TfiI GAWTC 1 cut(s) 372
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 4 cut(s) 7, 10, 101, 167
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 2 cut(s) 133, 309
TspGWI ACGGA 2 cut(s) 164, 176
TspMI CCCGGG 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 223
XapI RAATTY 1 cut(s) 323
XceI RCATGY 2 cut(s) 51, 206
XmaI CCCGGG 1 cut(s) 271
XmiI GTMKAC 2 cut(s) 44, 264
XspI CTAG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.