Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0014108

Physical Location & Seq

Genomic Coordinates Reverse (-)

23548107 .. 23548757

Gene Structure 651 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1142700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 651 bp

ATGAGACCACCGCCAACAGTGGGGCTGTCGGCGGGGGAGGTAGTCGAGTTTGGCCTTATCCACCTCCCTACGCCGCTGCCTTCCCCTCCAACAGGGCCAACGACATGCGTTTCCCACGCCTGCCGCTGGTGGCCTTACTCCGGCTCAAAAGAATTCGAGGCCAACGCCGCCATGATCATCATCGTCCTCCTCTCTGCTCTCATTTCCGCGTTGGGTCTTAACGCCGCCATCCGCTGCTTCCTCCGCGGCGGTAGAGGCTACCCTTCTGATCGTGAGAACAACAGTGTTCCTCAGAGTCGGCAGGCGTTGGTTGATGTTCAGCAGAAGAGCAATGTGGCAACGGCTTCGTGCTTAGAGGCGGCACCTACGGTGATTTACACGGCGGAGATGAAGGCGAAGCTGGCAGGGGTGGAGGCGGAGTGCGCCATTTGCTTGTCGGAGTTTGTGGAGGGGGAAGGGATTAGAGTTTTGCGGAGGTGTAAACATGGGTTTCATGCCCAGTGTATTCAAAAATGGTTGTCTTCTCACTCCTCTTGTCCCACGTGCCGCCGCACGTGCCTTCCTCAGTCTCCATCCCAATCCCCAGCACCACCACAACCCTCGGCGGACCATAATCCCGAACAAAATCCCGTGTCGGAGAGCAGTCCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

217

Amino Acids

23.11

Weight (kDa)

7.54

Isoelectric Point (pI)

69.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	140 - 183	1.7e-14	Ring finger domain
zf-RING_11	PF17123	140 - 169	2.8e-11	RING-like zinc finger
zf-C3HC4_2	PF13923	140 - 182	1.2e-09	Zinc finger, C3HC4 type (RING finger)
zf-RING_5	PF14634	140 - 184	8.1e-06	zinc-RING finger domain
zf-C3HC4	PF00097	141 - 182	2.1e-07	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	143 - 183	6.7e-08	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014108)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17245 AT5G47610
fragaria_vesca	FvH4_5g10181
malus_domestica	MD06G1127400.v1.1 MD10G1185300.v1.1 MD14G1142700.v1.1
prunus_persica	Prupe.5G137200_v2.0.a1
pyrus_communis	pycom06g11720
rosa_chinensis	RchiOBHm_Chr7g0188061
rosa_laevigata	RLG00000004730
rosa_multiflora	Rmu_ssc0000422.1_g000018
rosa_roxburghii	Rroxscaffold_3G00266770
rosa_rugosa	Rorug06G0487900
rosa_samantha	Rh7AG092200 Rh7BG093800 Rh7CG093100
rosa_wichuraiana	Rw7G007970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	361
AccII	CGCG	2 cut(s)	209, 246
AcsI	RAATTY	1 cut(s)	152
AcvI	CACGTG	2 cut(s)	543, 555
AfiI	CCNNNNNNNGG	4 cut(s)	20, 92, 126, 140
AgsI	TTSAA	1 cut(s)	509
AluBI	AGCT	1 cut(s)	400
AluI	AGCT	1 cut(s)	400
Alw26I	GTCTC	1 cut(s)	573
AoxI	GGCC	4 cut(s)	52, 95, 131, 159
ApeKI	GCWGC	2 cut(s)	76, 234
ApoI	RAATTY	1 cut(s)	152
AspLEI	GCGC	1 cut(s)	425
AspS9I	GGNCC	2 cut(s)	95, 607
AsuHPI	GGTGA	1 cut(s)	382
AvaII	GGWCC	1 cut(s)	607
BanI	GGYRCC	1 cut(s)	361
BbrPI	CACGTG	2 cut(s)	543, 555
BbsI	GAAGAC	1 cut(s)	513
BbvI	GCAGC	2 cut(s)	63, 221
BccI	CCATC	2 cut(s)	236, 580
BceAI	ACGGC	2 cut(s)	357, 396
BcgI	CGANNNNNNTGC	2 cut(s)	327, 361
BclI	TGATCA	1 cut(s)	174
BcoDI	GTCTC	1 cut(s)	573
Bme18I	GGWCC	1 cut(s)	607
BmgT120I	GGNCC	2 cut(s)	95, 607
BmiI	GGNNCC	1 cut(s)	363
BmrI	ACTGGG	1 cut(s)	493
BmuI	ACTGGG	1 cut(s)	493
BpiI	GAAGAC	1 cut(s)	513
BsaAI	YACGTR	2 cut(s)	543, 555
BsaBI	GATNNNNATC	1 cut(s)	179
BsaJI	CCNNGG	2 cut(s)	244, 600
BsaXI	ACNNNNNCTCC	2 cut(s)	404, 434
Bsc4I	CCNNNNNNNGG	4 cut(s)	20, 92, 126, 140
Bse1I	ACTGG	1 cut(s)	499
Bse3DI	GCAATG	1 cut(s)	337
Bse8I	GATNNNNATC	1 cut(s)	179
BseDI	CCNNGG	2 cut(s)	244, 600
BseGI	GGATG	2 cut(s)	228, 572
BseJI	GATNNNNATC	1 cut(s)	179
BseLI	CCNNNNNNNGG	4 cut(s)	20, 92, 126, 140
BseMI	GCAATG	1 cut(s)	337
BseMII	CTCAG	2 cut(s)	305, 578
BseNI	ACTGG	1 cut(s)	499
BseRI	GAGGAG	2 cut(s)	179, 520
BseXI	GCAGC	2 cut(s)	63, 221
BseYI	CCCAGC	1 cut(s)	583
Bsh1236I	CGCG	2 cut(s)	209, 246
BshFI	GGCC	4 cut(s)	54, 97, 133, 161
BshNI	GGYRCC	1 cut(s)	361
BsiSI	CCGG	1 cut(s)	141
BslFI	GGGAC	1 cut(s)	522
BslI	CCNNNNNNNGG	4 cut(s)	20, 92, 126, 140
BsmAI	GTCTC	1 cut(s)	573
BsmFI	GGGAC	1 cut(s)	522
BsnI	GGCC	4 cut(s)	54, 97, 133, 161
Bsp143I	GATC	2 cut(s)	174, 268
BspANI	GGCC	4 cut(s)	54, 97, 133, 161
BspCNI	CTCAG	2 cut(s)	304, 577
BspFNI	CGCG	2 cut(s)	209, 246
BspLI	GGNNCC	1 cut(s)	363
BspQI	GCTCTTC	1 cut(s)	320
BspT107I	GGYRCC	1 cut(s)	361
BsrDI	GCAATG	1 cut(s)	337
BsrI	ACTGG	1 cut(s)	499
BssECI	CCNNGG	2 cut(s)	244, 600
BssMI	GATC	2 cut(s)	174, 268
Bst4CI	ACNGT	3 cut(s)	19, 284, 370
Bst6I	CTCTTC	1 cut(s)	320
BstBAI	YACGTR	2 cut(s)	543, 555
BstC8I	GCNNGC	3 cut(s)	121, 303, 402
BstDEI	CTNAG	3 cut(s)	291, 352, 564
BstDSI	CCRYGG	1 cut(s)	244
BstENI	CCTNNNNNAGG	1 cut(s)	90
BstF5I	GGATG	2 cut(s)	228, 572
BstFNI	CGCG	2 cut(s)	209, 246
BstHHI	GCGC	1 cut(s)	425
BstKTI	GATC	2 cut(s)	177, 271
BstMAI	GTCTC	1 cut(s)	573
BstMBI	GATC	2 cut(s)	174, 268
BstMWI	GCNNNNNNNGC	7 cut(s)	167, 243, 255, 401, 422, 429, 555
BstNSI	RCATGY	1 cut(s)	108
BstUI	CGCG	2 cut(s)	209, 246
BstV1I	GCAGC	2 cut(s)	63, 221
BstV2I	GAAGAC	1 cut(s)	513
BsuRI	GGCC	4 cut(s)	54, 97, 133, 161
BtgI	CCRYGG	1 cut(s)	244
BtsCI	GGATG	2 cut(s)	228, 572
BtsIMutI	CAGTG	3 cut(s)	24, 289, 506
Cac8I	GCNNGC	3 cut(s)	121, 303, 402
CfoI	GCGC	1 cut(s)	425
Cfr13I	GGNCC	2 cut(s)	95, 607
Cfr42I	CCGCGG	1 cut(s)	247
CviAII	CATG	4 cut(s)	105, 172, 485, 494
CviJI	RGCY	9 cut(s)	25, 54, 97, 133, 144, 161, 258, 344, 400
CviKI_1	RGCY	9 cut(s)	25, 54, 97, 133, 144, 161, 258, 344, 400
DdeI	CTNAG	3 cut(s)	291, 352, 564
DpnI	GATC	2 cut(s)	176, 270
DpnII	GATC	2 cut(s)	174, 268
Eam1104I	CTCTTC	1 cut(s)	320
EarI	CTCTTC	1 cut(s)	320
EciI	GGCGGA	3 cut(s)	398, 431, 620
Eco47I	GGWCC	1 cut(s)	607
Eco72I	CACGTG	2 cut(s)	543, 555
EcoNI	CCTNNNNNAGG	1 cut(s)	90
EcoRI	GAATTC	1 cut(s)	152
FaeI	CATG	4 cut(s)	108, 175, 488, 497
FaiI	YATR	5 cut(s)	106, 173, 486, 495, 612
FaqI	GGGAC	1 cut(s)	522
FatI	CATG	4 cut(s)	104, 171, 484, 493
FauI	CCCGC	1 cut(s)	25
FbaI	TGATCA	1 cut(s)	174
FokI	GGATG	2 cut(s)	215, 559
GlaI	GCGC	1 cut(s)	424
GsaI	CCCAGC	1 cut(s)	587
HaeIII	GGCC	4 cut(s)	54, 97, 133, 161
HapII	CCGG	1 cut(s)	141
HhaI	GCGC	1 cut(s)	425
Hin1II	CATG	4 cut(s)	108, 175, 488, 497
Hin6I	GCGC	1 cut(s)	423
HinP1I	GCGC	1 cut(s)	423
HinfI	GANTC	1 cut(s)	295
HpaII	CCGG	1 cut(s)	141
HphI	GGTGA	1 cut(s)	382
Hpy166II	GTNNAC	1 cut(s)	482
Hpy188I	TCNGA	4 cut(s)	268, 294, 439, 637
Hpy188III	TCNNGA	2 cut(s)	272, 617
Hpy8I	GTNNAC	1 cut(s)	482
HpyAV	CCTTC	5 cut(s)	90, 273, 385, 449, 569
HpyCH4III	ACNGT	3 cut(s)	19, 284, 370
HpyCH4IV	ACGT	2 cut(s)	542, 554
HpyF10VI	GCNNNNNNNGC	7 cut(s)	167, 243, 255, 401, 422, 429, 555
HpyF3I	CTNAG	3 cut(s)	291, 352, 564
HpySE526I	ACGT	2 cut(s)	542, 554
Hsp92II	CATG	4 cut(s)	108, 175, 488, 497
HspAI	GCGC	1 cut(s)	423
Ksp22I	TGATCA	1 cut(s)	174
KspI	CCGCGG	1 cut(s)	247
Kzo9I	GATC	2 cut(s)	174, 268
LguI	GCTCTTC	1 cut(s)	320
LpnPI	CCDG	9 cut(s)	78, 112, 133, 154, 287, 386, 390, 512, 597
Lsp1109I	GCAGC	2 cut(s)	63, 221
MaeII	ACGT	2 cut(s)	542, 554
MalI	GATC	2 cut(s)	176, 270
MboI	GATC	2 cut(s)	174, 268
MboII	GAAGA	2 cut(s)	337, 513
MluCI	AATT	1 cut(s)	152
MlyI	GAGTC	1 cut(s)	304
MmeI	TCCRAC	3 cut(s)	113, 417, 615
MseI	TTAA	1 cut(s)	219
MspA1I	CMGCKG	4 cut(s)	76, 126, 234, 246
MspI	CCGG	1 cut(s)	141
MvnI	CGCG	2 cut(s)	209, 246
MwoI	GCNNNNNNNGC	7 cut(s)	167, 243, 255, 401, 422, 429, 555
NdeII	GATC	2 cut(s)	174, 268
NlaIII	CATG	4 cut(s)	108, 175, 488, 497
NlaIV	GGNNCC	1 cut(s)	363
NmeAIII	GCCGAG	1 cut(s)	581
NspI	RCATGY	1 cut(s)	108
PciSI	GCTCTTC	1 cut(s)	320
PleI	GAGTC	1 cut(s)	303
PmaCI	CACGTG	2 cut(s)	543, 555
PmlI	CACGTG	2 cut(s)	543, 555
PpsI	GAGTC	1 cut(s)	303
Ppu21I	YACGTR	2 cut(s)	543, 555
PspCI	CACGTG	2 cut(s)	543, 555
PspFI	CCCAGC	1 cut(s)	583
PspN4I	GGNNCC	1 cut(s)	363
PspPI	GGNCC	2 cut(s)	95, 607
SacII	CCGCGG	1 cut(s)	247
SapI	GCTCTTC	1 cut(s)	320
SaqAI	TTAA	1 cut(s)	219
Sau3AI	GATC	2 cut(s)	174, 268
Sau96I	GGNCC	2 cut(s)	95, 607
SchI	GAGTC	1 cut(s)	304
SetI	ASST	7 cut(s)	42, 66, 367, 402, 479, 545, 557
Sfr303I	CCGCGG	1 cut(s)	247
SgrBI	CCGCGG	1 cut(s)	247
SinI	GGWCC	1 cut(s)	607
Sse9I	AATT	1 cut(s)	152
TaaI	ACNGT	3 cut(s)	19, 284, 370
TaiI	ACGT	2 cut(s)	545, 557
TaqI	TCGA	2 cut(s)	45, 156
TasI	AATT	1 cut(s)	152
TauI	GCSGC	8 cut(s)	76, 126, 170, 227, 249, 362, 549, 552
Tru1I	TTAA	1 cut(s)	219
Tru9I	TTAA	1 cut(s)	219
TscAI	CASTG	3 cut(s)	24, 289, 506
TseI	GCWGC	2 cut(s)	76, 234
TspDTI	ATGAA	2 cut(s)	404, 482
TspGWI	ACGGA	1 cut(s)	636
TspRI	CASTG	3 cut(s)	24, 289, 506
VpaK11BI	GGWCC	1 cut(s)	607
XagI	CCTNNNNNAGG	1 cut(s)	90
XapI	RAATTY	1 cut(s)	152
XceI	RCATGY	1 cut(s)	108

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.