Rroxscaffold_3G00266770

RING-H2 finger protein ATL79-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014108
Physical Location & Seq
Forward (+)
59968251 .. 59968931
681 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00266770.1

Sequence Viewer

Length: 681 bp
ATGAGACCACCACCGAGAATACTACTAGAGGCGGCAGTAGCAGTAGCCGAGTTCGGCGGCACAGTCCACTTCTCTCCACCACCACTCTCCACCTCGCCACCTCCGACGCCAGCACCATGCGTGGCTCACGCCTGCCGCTGGTGGCCCTACTCCGGCTCTAAAGAGTTCGAGGCTAACGCAGCCATGGTCCTCATCATCCTCCTTTCGGCTCTCATCTGCGCCCTCGGCCTCAACGCCGCCATCCGCTGCTTCCTCCGCGGCACTCACCTAGTGGAGGGGCAGCTCGACCGCCGCAGCAGCAACCCTACCCAGACGCATCACGAGTTGGCTCGCGTGCAGCTCAAGACCAATGCGGCAGCGGCTCCCTGCTTTGATGGGGGACCCACGCTGGTGTACTCGCCGGAGATGAAGGCGGAGCTGGCAGGGGCAGCGGCGGAGTGCGCCATTTGCTTGTCGGAGTTCGTGGATGGGGAGGGGATAAGGGTGTTGGAGAGGTGTAAGCATGGGTTCCACGCCCACTGTATACAGGCTTGGCTGTCTTCTCACTCTTCTTGTCCAACGTGCCGTCGCACGTGCCTCCCTCCCTCCCCTTCTCATCCTCCTCCGCCGCCGCCACCGCCGCCGCAGCAATCCACCGAGGTGGAGGACCAACCGCCCTTGCCGGAAAGTATTGCTCCGTGA

Protein Analysis

226

Amino Acids

24.12

Weight (kDa)

6.35

Isoelectric Point (pI)

64.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 146 - 189 3.1e-14 Ring finger domain
zf-RING_11 PF17123 146 - 175 1.3e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 146 - 188 5e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 147 - 188 3.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 161 - 189 1.5e-07 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 162 - 191 1e-05 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17245 AT5G47610
fragaria_vesca FvH4_5g10181
malus_domestica MD06G1127400.v1.1 MD10G1185300.v1.1 MD14G1142700.v1.1
prunus_persica Prupe.5G137200_v2.0.a1
pyrus_communis pycom06g11720
rosa_chinensis RchiOBHm_Chr7g0188061
rosa_laevigata RLG00000004730
rosa_multiflora Rmu_ssc0000422.1_g000018
rosa_roxburghii Rroxscaffold_3G00266770
rosa_rugosa Rorug06G0487900
rosa_samantha Rh7AG092200 Rh7BG093800 Rh7CG093100
rosa_wichuraiana Rw7G007970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 523
AccII CGCG 2 cut(s) 258, 333
AcvI CACGTG 1 cut(s) 573
AcyI GRCGYC 1 cut(s) 107
AdeI CACNNNGTG 1 cut(s) 271
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 4 cut(s) 138, 152, 205, 274
AleI CACNNNNGTG 2 cut(s) 389, 638
AluBI AGCT 3 cut(s) 283, 340, 418
AluI AGCT 3 cut(s) 283, 340, 418
AoxI GGCC 2 cut(s) 143, 226
ApeKI GCWGC 9 cut(s) 179, 246, 280, 294, 297, 337, 356, 428, 625
AspLEI GCGC 2 cut(s) 221, 443
AspS9I GGNCC 4 cut(s) 144, 187, 380, 646
AsuHPI GGTGA 1 cut(s) 257
AvaII GGWCC 3 cut(s) 187, 380, 646
BauI CACGAG 1 cut(s) 320
BbrPI CACGTG 1 cut(s) 573
BbsI GAAGAC 1 cut(s) 531
BbvI GCAGC 9 cut(s) 191, 233, 292, 306, 309, 349, 368, 440, 637
BccI CCATC 3 cut(s) 248, 368, 461
BceAI ACGGC 1 cut(s) 549
BfaI CTAG 2 cut(s) 26, 269
Bme18I GGWCC 3 cut(s) 187, 380, 646
BmgT120I GGNCC 4 cut(s) 144, 187, 380, 646
BmiI GGNNCC 4 cut(s) 363, 381, 382, 509
BmsI GCATC 1 cut(s) 325
BpiI GAAGAC 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 326
BsaAI YACGTR 1 cut(s) 573
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 4 cut(s) 183, 223, 256, 636
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 152, 205, 274
BseDI CCNNGG 4 cut(s) 183, 223, 256, 636
BseGI GGATG 4 cut(s) 195, 240, 472, 595
BseLI CCNNNNNNNGG 4 cut(s) 138, 152, 205, 274
BseRI GAGGAG 1 cut(s) 591
BseXI GCAGC 9 cut(s) 191, 233, 292, 306, 309, 349, 368, 440, 637
BsgI GTGCAG 1 cut(s) 356
Bsh1236I CGCG 2 cut(s) 258, 333
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 2 cut(s) 145, 228
BsiEI CGRYCG 1 cut(s) 289
BsiSI CCGG 3 cut(s) 153, 401, 662
BslFI GGGAC 1 cut(s) 393
BslI CCNNNNNNNGG 4 cut(s) 138, 152, 205, 274
BsmFI GGGAC 1 cut(s) 393
BsnI GGCC 2 cut(s) 145, 228
Bsp19I CCATGG 1 cut(s) 183
BspANI GGCC 2 cut(s) 145, 228
BspFNI CGCG 2 cut(s) 258, 333
BspLI GGNNCC 4 cut(s) 363, 381, 382, 509
BssECI CCNNGG 4 cut(s) 183, 223, 256, 636
BssNAI GTATAC 1 cut(s) 524
BssNI GRCGYC 1 cut(s) 107
BssSI CACGAG 1 cut(s) 320
BssT1I CCWWGG 1 cut(s) 183
Bst1107I GTATAC 1 cut(s) 524
Bst2BI CACGAG 1 cut(s) 320
Bst4CI ACNGT 2 cut(s) 64, 521
Bst6I CTCTTC 1 cut(s) 553
BstACI GRCGYC 1 cut(s) 107
BstBAI YACGTR 1 cut(s) 573
BstC8I GCNNGC 5 cut(s) 111, 133, 331, 335, 420
BstDSI CCRYGG 2 cut(s) 183, 256
BstENI CCTNNNNNAGG 1 cut(s) 272
BstF5I GGATG 4 cut(s) 195, 240, 472, 595
BstFNI CGCG 2 cut(s) 258, 333
BstHHI GCGC 2 cut(s) 221, 443
BstMCI CGRYCG 1 cut(s) 289
BstUI CGCG 2 cut(s) 258, 333
BstV1I GCAGC 9 cut(s) 191, 233, 292, 306, 309, 349, 368, 440, 637
BstV2I GAAGAC 1 cut(s) 531
BstXI CCANNNNNNTGG 1 cut(s) 640
BstZ17I GTATAC 1 cut(s) 524
BsuRI GGCC 2 cut(s) 145, 228
BtgI CCRYGG 2 cut(s) 183, 256
BtsCI GGATG 4 cut(s) 195, 240, 472, 595
BtsIMutI CAGTG 1 cut(s) 517
Cac8I GCNNGC 5 cut(s) 111, 133, 331, 335, 420
CfoI GCGC 2 cut(s) 221, 443
Cfr13I GGNCC 4 cut(s) 144, 187, 380, 646
Cfr42I CCGCGG 1 cut(s) 259
CseI GACGC 2 cut(s) 115, 322
Csp6I GTAC 1 cut(s) 394
CviAII CATG 3 cut(s) 117, 184, 503
CviQI GTAC 1 cut(s) 394
DraIII CACNNNGTG 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 553
EarI CTCTTC 1 cut(s) 553
EciI GGCGGA 3 cut(s) 428, 449, 594
Eco130I CCWWGG 1 cut(s) 183
Eco47I GGWCC 3 cut(s) 187, 380, 646
Eco72I CACGTG 1 cut(s) 573
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 380
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 3 cut(s) 120, 187, 506
FaiI YATR 4 cut(s) 118, 185, 504, 524
FaqI GGGAC 1 cut(s) 393
FatI CATG 3 cut(s) 116, 183, 502
FblI GTMKAC 1 cut(s) 523
FokI GGATG 4 cut(s) 182, 227, 479, 582
FspBI CTAG 2 cut(s) 26, 269
GlaI GCGC 2 cut(s) 220, 442
HaeIII GGCC 2 cut(s) 145, 228
HapII CCGG 3 cut(s) 153, 401, 662
HgaI GACGC 2 cut(s) 115, 322
HhaI GCGC 2 cut(s) 221, 443
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 3 cut(s) 120, 187, 506
Hin6I GCGC 2 cut(s) 219, 441
HinP1I GCGC 2 cut(s) 219, 441
HpaII CCGG 3 cut(s) 153, 401, 662
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 3 cut(s) 67, 394, 524
Hpy188I TCNGA 2 cut(s) 105, 457
Hpy188III TCNNGA 2 cut(s) 320, 343
Hpy8I GTNNAC 3 cut(s) 67, 394, 524
Hpy99I CGWCG 2 cut(s) 109, 570
HpyAV CCTTC 2 cut(s) 403, 600
HpyCH4III ACNGT 2 cut(s) 64, 521
HpyCH4IV ACGT 2 cut(s) 560, 572
HpyCH4V TGCA 1 cut(s) 337
HpySE526I ACGT 2 cut(s) 560, 572
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 3 cut(s) 120, 187, 506
HspAI GCGC 2 cut(s) 219, 441
KflI GGGWCCC 1 cut(s) 380
KspI CCGCGG 1 cut(s) 259
LmnI GCTCC 3 cut(s) 367, 415, 679
Lsp1109I GCAGC 9 cut(s) 191, 233, 292, 306, 309, 349, 368, 440, 637
LweI GCATC 1 cut(s) 325
MaeI CTAG 2 cut(s) 26, 269
MaeII ACGT 2 cut(s) 560, 572
MboII GAAGA 2 cut(s) 531, 540
MmeI TCCRAC 4 cut(s) 128, 435, 468, 581
MslI CAYNNNNRTG 2 cut(s) 389, 638
MspA1I CMGCKG 5 cut(s) 138, 246, 258, 359, 431
MspI CCGG 3 cut(s) 153, 401, 662
MvnI CGCG 2 cut(s) 258, 333
NcoI CCATGG 1 cut(s) 183
NlaIII CATG 3 cut(s) 120, 187, 506
NlaIV GGNNCC 4 cut(s) 363, 381, 382, 509
NmeAIII GCCGAG 2 cut(s) 73, 204
OliI CACNNNNGTG 2 cut(s) 389, 638
PcsI WCGNNNNNNNCGW 1 cut(s) 101
PmaCI CACGTG 1 cut(s) 573
PmlI CACGTG 1 cut(s) 573
Ppu21I YACGTR 1 cut(s) 573
PpuMI RGGWCCY 1 cut(s) 380
Psp5II RGGWCCY 1 cut(s) 380
PspCI CACGTG 1 cut(s) 573
PspN4I GGNNCC 4 cut(s) 363, 381, 382, 509
PspPI GGNCC 4 cut(s) 144, 187, 380, 646
PspPPI RGGWCCY 1 cut(s) 380
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 2 cut(s) 389, 638
SacII CCGCGG 1 cut(s) 259
Sau96I GGNCC 4 cut(s) 144, 187, 380, 646
SfaNI GCATC 1 cut(s) 325
Sfr303I CCGCGG 1 cut(s) 259
SgrBI CCGCGG 1 cut(s) 259
SinI GGWCC 3 cut(s) 187, 380, 646
SmiMI CAYNNNNRTG 2 cut(s) 389, 638
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
SspMI CTAG 2 cut(s) 26, 269
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 2 cut(s) 64, 521
TaiI ACGT 2 cut(s) 563, 575
TaqI TCGA 2 cut(s) 168, 285
TatI WGTACW 1 cut(s) 393
TscAI CASTG 1 cut(s) 524
TseI GCWGC 9 cut(s) 179, 246, 280, 294, 297, 337, 356, 428, 625
TspDTI ATGAA 1 cut(s) 422
TspGWI ACGGA 1 cut(s) 666
TspRI CASTG 1 cut(s) 524
VpaK11BI GGWCC 3 cut(s) 187, 380, 646
XagI CCTNNNNNAGG 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 523
XspI CTAG 2 cut(s) 26, 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.