MD06G1127400.v1.1

RING-H2 finger protein ATL79-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014108
Physical Location & Seq
Reverse (-)
26934124 .. 26934774
651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1127400.v1.1.491

Sequence Viewer

Length: 651 bp
ATGAGACCACCGCCAACAGTGGGGCTGTCAGCTGTGGAAGTAGCTGATGTTAACCTCATCCACCTTTCTCCGCCACTTCCTTCACCTCCAGCAGGGCCAACGACATGCGGCTCCCACGCCTGCCGCTGGTGGCCTTACTCCGGCTCAAAAGAATTCGAGGCCAACGCCGCCATGATCATCATCGTCCTCCTTTGCGCTCTCATTTGCGCGTTGGGTCTTAATGCCGCCATCCGCTGCTTCGTCCGCGGTGGCAGCACCAACCCTTCCGATCGTCAGAACAACAGTGCTCCTCAGAGACAACAGGCGTTGGTTGATGTTCAGCAGAAGAGCAATGTGGCAGCGGCTAAGTGCTTAGAGGCGGCTCCGGCGGTGATTTACACGGCAGAGATGAAGGCGAAGCTGGCAGGGGTGGAGGCGGAGTGCGCCATTTGCTTGTCGGAGTTTGTGGAGGGGGAAGGGATTAGAGTGATAGGCAGGTGTAAACATGGTTTTCATGCTCAGTGTATTCAGGAATGGTTGTCTTCTCACTCCTCTTGTCCCACGTGCCGACGCACGTGCCTTCCTCAGTCTCCGTCTCAGTCACCACCACCTCCGCAACCCGCCAGGGAGCATAGTTCCGAACAAAATCCTATGCCGGAAAGCGGCCCGTAG

Protein Analysis

217

Amino Acids

22.96

Weight (kDa)

6.97

Isoelectric Point (pI)

64.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 140 - 183 3.9e-14 Ring finger domain
zf-RING_11 PF17123 140 - 169 2.6e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 140 - 182 1.1e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 140 - 184 4.8e-06 zinc-RING finger domain
zf-rbx1 PF12678 141 - 183 4.4e-09 RING-H2 zinc finger domain
zf-C3HC4 PF00097 141 - 182 2.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 156 - 185 6.4e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17245 AT5G47610
fragaria_vesca FvH4_5g10181
malus_domestica MD06G1127400.v1.1 MD10G1185300.v1.1 MD14G1142700.v1.1
prunus_persica Prupe.5G137200_v2.0.a1
pyrus_communis pycom06g11720
rosa_chinensis RchiOBHm_Chr7g0188061
rosa_laevigata RLG00000004730
rosa_multiflora Rmu_ssc0000422.1_g000018
rosa_roxburghii Rroxscaffold_3G00266770
rosa_rugosa Rorug06G0487900
rosa_samantha Rh7AG092200 Rh7BG093800 Rh7CG093100
rosa_wichuraiana Rw7G007970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 465
Acc36I ACCTGC 1 cut(s) 465
AccII CGCG 2 cut(s) 209, 246
AcsI RAATTY 1 cut(s) 152
AcvI CACGTG 2 cut(s) 543, 555
AfiI CCNNNNNNNGG 5 cut(s) 20, 92, 126, 140, 641
AjnI CCWGG 1 cut(s) 602
AluBI AGCT 3 cut(s) 32, 44, 400
AluI AGCT 3 cut(s) 32, 44, 400
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 3 cut(s) 289, 573, 579
AoxI GGCC 4 cut(s) 95, 131, 159, 643
ApeKI GCWGC 3 cut(s) 234, 252, 338
ApoI RAATTY 1 cut(s) 152
AspLEI GCGC 3 cut(s) 197, 209, 425
AspS9I GGNCC 2 cut(s) 95, 644
AsuHPI GGTGA 3 cut(s) 75, 382, 573
BbrPI CACGTG 2 cut(s) 543, 555
BbsI GAAGAC 1 cut(s) 513
Bbv12I GWGCWC 1 cut(s) 289
BbvI GCAGC 3 cut(s) 221, 264, 350
BccI CCATC 1 cut(s) 236
BceAI ACGGC 1 cut(s) 396
BcgI CGANNNNNNTGC 2 cut(s) 537, 571
BciT130I CCWGG 1 cut(s) 604
BclI TGATCA 1 cut(s) 174
BcoDI GTCTC 3 cut(s) 289, 573, 579
BfuAI ACCTGC 1 cut(s) 465
Bme1390I CCNGG 1 cut(s) 604
BmgT120I GGNCC 2 cut(s) 95, 644
BmiI GGNNCC 2 cut(s) 112, 363
BmrFI CCNGG 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 513
BpmI CTGGAG 1 cut(s) 72
BsaAI YACGTR 2 cut(s) 543, 555
BsaBI GATNNNNATC 1 cut(s) 179
BsaJI CCNNGG 2 cut(s) 244, 603
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 92, 126, 140, 641
Bse3DI GCAATG 1 cut(s) 337
Bse8I GATNNNNATC 1 cut(s) 179
BseBI CCWGG 1 cut(s) 604
BseDI CCNNGG 2 cut(s) 244, 603
BseGI GGATG 2 cut(s) 57, 228
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 5 cut(s) 20, 92, 126, 140, 641
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 4 cut(s) 305, 512, 578, 590
BseRI GAGGAG 2 cut(s) 279, 520
BseXI GCAGC 3 cut(s) 221, 264, 350
Bsh1236I CGCG 2 cut(s) 209, 246
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 4 cut(s) 97, 133, 161, 645
BsiEI CGRYCG 1 cut(s) 271
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 3 cut(s) 141, 365, 635
BslFI GGGAC 1 cut(s) 522
BslI CCNNNNNNNGG 5 cut(s) 20, 92, 126, 140, 641
BsmAI GTCTC 3 cut(s) 289, 573, 579
BsmBI CGTCTC 1 cut(s) 579
BsmFI GGGAC 1 cut(s) 522
BsnI GGCC 4 cut(s) 97, 133, 161, 645
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 2 cut(s) 174, 268
BspANI GGCC 4 cut(s) 97, 133, 161, 645
BspCNI CTCAG 4 cut(s) 304, 511, 577, 589
BspFNI CGCG 2 cut(s) 209, 246
BspLI GGNNCC 2 cut(s) 112, 363
BspMI ACCTGC 1 cut(s) 465
BspQI GCTCTTC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 337
BssECI CCNNGG 2 cut(s) 244, 603
BssMI GATC 2 cut(s) 174, 268
Bst2UI CCWGG 1 cut(s) 604
Bst4CI ACNGT 2 cut(s) 19, 284
Bst6I CTCTTC 1 cut(s) 320
BstBAI YACGTR 2 cut(s) 543, 555
BstC8I GCNNGC 2 cut(s) 121, 402
BstDEI CTNAG 6 cut(s) 291, 345, 352, 498, 564, 576
BstDSI CCRYGG 1 cut(s) 244
BstENI CCTNNNNNAGG 1 cut(s) 90
BstF5I GGATG 2 cut(s) 57, 228
BstFNI CGCG 2 cut(s) 209, 246
BstHHI GCGC 3 cut(s) 197, 209, 425
BstKTI GATC 2 cut(s) 177, 271
BstMAI GTCTC 3 cut(s) 289, 573, 579
BstMBI GATC 2 cut(s) 174, 268
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 7 cut(s) 167, 243, 252, 365, 401, 422, 429
BstNI CCWGG 1 cut(s) 604
BstNSI RCATGY 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 602
BstUI CGCG 2 cut(s) 209, 246
BstV1I GCAGC 3 cut(s) 221, 264, 350
BstV2I GAAGAC 1 cut(s) 513
BsuRI GGCC 4 cut(s) 97, 133, 161, 645
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 2 cut(s) 57, 228
BtsIMutI CAGTG 3 cut(s) 24, 289, 506
BveI ACCTGC 1 cut(s) 465
Cac8I GCNNGC 2 cut(s) 121, 402
CfoI GCGC 3 cut(s) 197, 209, 425
Cfr13I GGNCC 2 cut(s) 95, 644
Cfr42I CCGCGG 1 cut(s) 247
CseI GACGC 1 cut(s) 558
CviAII CATG 4 cut(s) 105, 172, 485, 494
DdeI CTNAG 6 cut(s) 291, 345, 352, 498, 564, 576
DpnI GATC 2 cut(s) 176, 270
DpnII GATC 2 cut(s) 174, 268
Eam1104I CTCTTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 320
EciI GGCGGA 2 cut(s) 60, 431
Eco72I CACGTG 2 cut(s) 543, 555
EcoNI CCTNNNNNAGG 1 cut(s) 90
EcoRI GAATTC 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 602
Esp3I CGTCTC 1 cut(s) 579
FaeI CATG 4 cut(s) 108, 175, 488, 497
FaiI YATR 6 cut(s) 106, 173, 486, 495, 612, 632
FaqI GGGAC 1 cut(s) 522
FatI CATG 4 cut(s) 104, 171, 484, 493
FauI CCCGC 1 cut(s) 607
FbaI TGATCA 1 cut(s) 174
FokI GGATG 2 cut(s) 44, 215
GlaI GCGC 3 cut(s) 196, 208, 424
GsuI CTGGAG 1 cut(s) 72
HaeIII GGCC 4 cut(s) 97, 133, 161, 645
HapII CCGG 3 cut(s) 141, 365, 635
HgaI GACGC 1 cut(s) 558
HhaI GCGC 3 cut(s) 197, 209, 425
Hin1II CATG 4 cut(s) 108, 175, 488, 497
Hin6I GCGC 3 cut(s) 195, 207, 423
HinP1I GCGC 3 cut(s) 195, 207, 423
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HpaI GTTAAC 1 cut(s) 52
HpaII CCGG 3 cut(s) 141, 365, 635
HphI GGTGA 3 cut(s) 75, 382, 573
Hpy166II GTNNAC 2 cut(s) 52, 482
Hpy188I TCNGA 5 cut(s) 268, 276, 294, 439, 619
Hpy188III TCNNGA 1 cut(s) 509
Hpy8I GTNNAC 2 cut(s) 52, 482
Hpy99I CGWCG 1 cut(s) 552
HpyAV CCTTC 5 cut(s) 90, 273, 385, 449, 569
HpyCH4III ACNGT 2 cut(s) 19, 284
HpyCH4IV ACGT 2 cut(s) 542, 554
HpyF10VI GCNNNNNNNGC 7 cut(s) 167, 243, 252, 365, 401, 422, 429
HpyF3I CTNAG 6 cut(s) 291, 345, 352, 498, 564, 576
HpySE526I ACGT 2 cut(s) 542, 554
Hsp92II CATG 4 cut(s) 108, 175, 488, 497
HspAI GCGC 3 cut(s) 195, 207, 423
Ksp22I TGATCA 1 cut(s) 174
KspAI GTTAAC 1 cut(s) 52
KspI CCGCGG 1 cut(s) 247
Kzo9I GATC 2 cut(s) 174, 268
LguI GCTCTTC 1 cut(s) 320
LmnI GCTCC 4 cut(s) 116, 292, 367, 607
Lsp1109I GCAGC 3 cut(s) 221, 264, 350
MaeII ACGT 2 cut(s) 542, 554
MaeIII GTNAC 1 cut(s) 579
MalI GATC 2 cut(s) 176, 270
MboI GATC 2 cut(s) 174, 268
MboII GAAGA 2 cut(s) 337, 513
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 1 cut(s) 152
MmeI TCCRAC 1 cut(s) 417
MseI TTAA 2 cut(s) 51, 219
MspA1I CMGCKG 5 cut(s) 32, 126, 234, 246, 341
MspI CCGG 3 cut(s) 141, 365, 635
MspR9I CCNGG 1 cut(s) 604
MvaI CCWGG 1 cut(s) 604
MvnI CGCG 2 cut(s) 209, 246
MwoI GCNNNNNNNGC 7 cut(s) 167, 243, 252, 365, 401, 422, 429
NdeII GATC 2 cut(s) 174, 268
NlaIII CATG 4 cut(s) 108, 175, 488, 497
NlaIV GGNNCC 2 cut(s) 112, 363
NmuCI GTSAC 1 cut(s) 579
NspI RCATGY 1 cut(s) 108
PaqCI CACCTGC 1 cut(s) 465
PciSI GCTCTTC 1 cut(s) 320
Ple19I CGATCG 1 cut(s) 271
PmaCI CACGTG 2 cut(s) 543, 555
PmlI CACGTG 2 cut(s) 543, 555
Ppu21I YACGTR 2 cut(s) 543, 555
Psp6I CCWGG 1 cut(s) 602
PspCI CACGTG 2 cut(s) 543, 555
PspGI CCWGG 1 cut(s) 602
PspN4I GGNNCC 2 cut(s) 112, 363
PspPI GGNCC 2 cut(s) 95, 644
PvuI CGATCG 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 32
SacII CCGCGG 1 cut(s) 247
SapI GCTCTTC 1 cut(s) 320
SaqAI TTAA 2 cut(s) 51, 219
Sau3AI GATC 2 cut(s) 174, 268
Sau96I GGNCC 2 cut(s) 95, 644
ScrFI CCNGG 1 cut(s) 604
SduI GDGCHC 1 cut(s) 289
Sfr303I CCGCGG 1 cut(s) 247
SgrBI CCGCGG 1 cut(s) 247
Sse9I AATT 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 602
TaaI ACNGT 2 cut(s) 19, 284
TaiI ACGT 2 cut(s) 545, 557
TaqI TCGA 1 cut(s) 156
TasI AATT 1 cut(s) 152
TauI GCSGC 7 cut(s) 111, 126, 170, 227, 344, 362, 645
Tru1I TTAA 2 cut(s) 51, 219
Tru9I TTAA 2 cut(s) 51, 219
TscAI CASTG 3 cut(s) 24, 289, 506
TseFI GTSAC 1 cut(s) 579
TseI GCWGC 3 cut(s) 234, 252, 338
Tsp45I GTSAC 1 cut(s) 579
TspDTI ATGAA 2 cut(s) 404, 482
TspGWI ACGGA 1 cut(s) 561
TspRI CASTG 3 cut(s) 24, 289, 506
XagI CCTNNNNNAGG 1 cut(s) 90
XapI RAATTY 1 cut(s) 152
XceI RCATGY 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.