AT4G18300

Translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005877
Physical Location & Seq
Forward (+)
10118784 .. 10121258
2475 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18300.1

Sequence Viewer

Length: 2130 bp
ATGGGTGCTCAGAAGAAACCCGCGGCGGCTAAGGTTCGTGAGGATTCAGAGGATCTAAGTCGTCAACGATTACAAGCCATTCTTCTAGCCGACAGCTTCACCACTCAATTCCGTTCCATAACCCTAGAACGACCAAAGGTACTATTGCCGATAGTGAACATCCCGATGATTGATTACACTTTAGCATGGCTCGAATCAGCTGGGATCGAAGAGGTTTTTGTGTTCTGTTGTGCTCATTCGTCGCAGATCATCGATTACCTGAAGAAATCGGAATGGTACACACGCCCAAACCCAAACTTGTTGGTGAGAACGATAGTGTCTCACAATTCCACCAGTGTTGGAGATGCTCTGCGTTATATCTATGAACAACAGACAGAGACGTCGCAGATTCAAGGTGATTTTGTTCTTGTTAGTGGTGATACTGTTAGTAATATGCCACTTGCTGATTTGATTCAACAACATAGAGATAGGAAGAAGAAAGATGAGAAAGCTATCATGACTATGGTTTTTAAGAAGCAGTCTAGACTAGGAATAGGATCTGATCAACTCTTTGTTGCAGTAGATCCTTTAACGAAACAGCTTCTTCATTACGAGGAATGTAATTCGAGAGGCGGGGACTTTTGCTTAGATAAGTCCTTGTTGGATAGTACTGTTTTATGCAGTGATATGCAGGATTGTTACATTGACATTTGTTCTCCTGAGGTGCTTAGTCTCTTTGAGGATAACTTTGATTATCAGCATATGCGCCGTCATTTTGTTAATGGTTTGCTTGTTGATGATATTATGGGTTACAAGATTTTCACTCATGAGATTCAGTCGAGTTGTTATGCTGCAAGGATCGACAATTTACGGAGCTATGACACTGTTAGCAAGGATATAATCCAGAGGTGGACATACCCGTACGTACCTAATATCGATTTCAGCGGGAATCGCCCTGTAAAGCTCGGGAGACGGGGGATATACCGGGCTTCTGATGCAGTTCAATCACACACTGCAGATGTTGGAGCTTCCACTGTCATTGGATATGGCACCAAAATTGGTAATGGAGGTAAAATCTATAACTCTGTTATTGGGAACGGATGTTGTATTGGATCAAATGTGGTGATACAAGGCTCATACATATGGAACAATGTTACGATTGAAGATGGGTGTGAGATAAGGAACGCTATTGTCTGTGATGGTGTGAAAGTCAGAGCAGGAGCTGTTCTGCAGCCTGGTGTTGTTTTGTCTTTCAGTGTTGTAGTTGGGCGGGACTTTGTTGTACCTGCATATTCAAAGGTCTCTTTACTTCAACAGCCAACTACAGAAGACAGCGATGAAGAGCTGGAATATGCTGATAGTAGCAGCGGGACTGCAGATCATTCGTCAGGCCTAAATAATTTGCAAATTGAGTCCAAAGCATCTGAGCTTGGTCCTGATGGAGCAGGTTACATCTGGGAAATATGTGACAGCGCTCATGATGAGGAGTGGAAACATTCTGTTGTACCAATCCCCATGGATAAACTTGCCGATATCACTGGGGCCATGGACGATGATGACATAGAGGATGACATTGTTGTCCCACCTTCTGGAGAGTTGAAATCTGATACTGATATTATTAACACTGATGCAAATGATTCCTATGGCGACTTTGAGAGAGAGGTTGAAGAAGAATTCTTAAGTGCCGTTGAAAAGGATAACAAAGCGGACATTGTAATTCCTTTGATTAACTCACACCGGTTGGCGTACAACATGGACTCTGCAGATTGTGCTGGAGCAGTATTCTACTCTATGATGAGACTGGCAGTAAAGAGTCCACATAATTCTGCTACTGAATTGTACAGAAACGCGATGGGTGTCATAACTAAATGGAAGGGAGTTCTTGGGTTCTATTTAAAGCAAACTGATGCACAGATAGAAGTGATAATGAAATTCGAAGAGATGTGTCAGGAATCTGAAGAGTTGAGCCCTTTGTTTGCTAAACTTCTGCCTTTTCTGTATGACAAAGACGTGGTGCAAGAAGATGCGATCTTGAGATGGGGGGAAGAGAAAGCAGGAGCAGACGATTGTGACAAAGTTTATCTGAATAAATGTGAGTCATTCATAAAGTGGCTCAAGGAAGCATCTGAGGAGGAAGAAGAAGATAGTTAG

Protein Analysis

709

Amino Acids

79.24

Weight (kDa)

4.61

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 26 - 177 1.5e-10 Nucleotidyl transferase
LbH_EIF2B PF25084 317 - 421 3.5e-35 EIF2B subunit epsilon LbH domain
GMPPB_C PF25087 328 - 412 6.6e-08 GMPPB C-terminal domain
Hexapep_GlmU PF24894 343 - 402 3.6e-07 GlmU-like, C-terminal hexapeptide
W2 PF02020 630 - 708 3.4e-21 eIF4-gamma/eIF5/eIF2-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 379, 1556
AatII GACGTC 1 cut(s) 383
Acc36I ACCTGC 2 cut(s) 1273, 1415
AccB1I GGYRCC 1 cut(s) 1028
AccB7I CCANNNNNTGG 1 cut(s) 1568
AccII CGCG 2 cut(s) 23, 1829
AciI CCGC 8 cut(s) 21, 23, 26, 612, 924, 1249, 1347, 1685
AclWI GGATC 6 cut(s) 60, 212, 544, 557, 845, 1099
AcsI RAATTY 2 cut(s) 1652, 1910
AcuI CTGAAG 2 cut(s) 281, 1956
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 9 cut(s) 141, 278, 649, 902, 906, 1263, 1485, 1727, 1820
AfeI AGCGCT 1 cut(s) 1453
AfiI CCNNNNNNNGG 1 cut(s) 1568
AflII CTTAAG 1 cut(s) 1657
AgeI ACCGGT 1 cut(s) 1716
AgsI TTSAA 9 cut(s) 392, 455, 983, 1142, 1275, 1292, 1579, 1646, 1670
AjiI CACGTC 1 cut(s) 1990
AjnI CCWGG 1 cut(s) 1213
Alw21I GWGCWC 2 cut(s) 10, 235
Alw26I GTCTC 6 cut(s) 324, 371, 716, 943, 1285, 1771
AlwI GGATC 6 cut(s) 60, 212, 544, 557, 845, 1099
AlwNI CAGNNNCTG 1 cut(s) 1202
Ama87I CYCGRG 1 cut(s) 944
Aor51HI AGCGCT 1 cut(s) 1453
AoxI GGCC 2 cut(s) 1369, 1521
ApeKI GCWGC 3 cut(s) 830, 1210, 1344
ApoI RAATTY 2 cut(s) 1652, 1910
AsiGI ACCGGT 1 cut(s) 1716
AspLEI GCGC 2 cut(s) 747, 1454
AspS9I GGNCC 2 cut(s) 1412, 1521
AsuC2I CCSGG 1 cut(s) 965
AsuHPI GGTGA 5 cut(s) 91, 316, 407, 428, 1114
AsuII TTCGAA 1 cut(s) 1914
AvaI CYCGRG 1 cut(s) 944
AvaII GGWCC 1 cut(s) 1412
AxyI CCTNAGG 1 cut(s) 699
BanI GGYRCC 1 cut(s) 1028
BanII GRGCYC 1 cut(s) 1949
BbsI GAAGAC 1 cut(s) 1314
Bbv12I GWGCWC 2 cut(s) 10, 235
BbvI GCAGC 3 cut(s) 817, 1222, 1356
BccI CCATC 5 cut(s) 1139, 1172, 1412, 1825, 2010
BceAI ACGGC 2 cut(s) 732, 1649
BcgI CGANNNNNNTGC 2 cut(s) 1344, 1378
BciT130I CCWGG 1 cut(s) 1215
BclI TGATCA 1 cut(s) 541
BcnI CCSGG 1 cut(s) 965
BcoDI GTCTC 6 cut(s) 324, 371, 716, 943, 1285, 1771
BfaI CTAG 4 cut(s) 86, 125, 522, 527
BfmI CTRYAG 5 cut(s) 993, 1208, 1302, 1353, 1740
BfoI RGCGCY 1 cut(s) 1455
BfrI CTTAAG 1 cut(s) 1657
BfuAI ACCTGC 2 cut(s) 1273, 1415
BisI GCNGC 5 cut(s) 24, 27, 831, 1211, 1345
BlsI GCNGC 5 cut(s) 25, 28, 832, 1212, 1346
BmcAI AGTACT 1 cut(s) 649
Bme1390I CCNGG 2 cut(s) 965, 1215
Bme18I GGWCC 1 cut(s) 1412
BmeT110I CYCGRG 1 cut(s) 944
BmgBI CACGTC 1 cut(s) 1990
BmgT120I GGNCC 2 cut(s) 1412, 1521
BmiI GGNNCC 2 cut(s) 1030, 1522
BmrFI CCNGG 2 cut(s) 965, 1215
BmrI ACTGGG 1 cut(s) 1527
BmsI GCATC 7 cut(s) 334, 964, 1409, 1597, 1876, 1993, 2111
BmuI ACTGGG 1 cut(s) 1527
BpiI GAAGAC 1 cut(s) 1314
BpmI CTGGAG 2 cut(s) 1590, 1773
Bpu10I CCTNAGC 1 cut(s) 30
Bpu14I TTCGAA 1 cut(s) 1914
BpuEI CTTGAG 2 cut(s) 2032, 2078
BpuMI CCSGG 1 cut(s) 965
Bsa29I ATCGAT 2 cut(s) 252, 915
BsaAI YACGTR 1 cut(s) 904
BsaHI GRCGYC 1 cut(s) 380
BsaI GGTCTC 1 cut(s) 1285
BsaJI CCNNGG 3 cut(s) 21, 1494, 1524
BsaWI WCCGGW 1 cut(s) 1716
Bsc4I CCNNNNNNNGG 1 cut(s) 1568
Bse118I RCCGGY 1 cut(s) 1716
Bse1I ACTGG 3 cut(s) 333, 1522, 1785
Bse21I CCTNAGG 1 cut(s) 699
BseBI CCWGG 1 cut(s) 1215
BseCI ATCGAT 2 cut(s) 252, 915
BseDI CCNNGG 3 cut(s) 21, 1494, 1524
BseGI GGATG 3 cut(s) 159, 1085, 1552
BseLI CCNNNNNNNGG 1 cut(s) 1568
BseMII CTCAG 4 cut(s) 23, 690, 1395, 2097
BseNI ACTGG 3 cut(s) 333, 1522, 1785
BseRI GAGGAG 2 cut(s) 1478, 2123
BseXI GCAGC 3 cut(s) 817, 1222, 1356
BseYI CCCAGC 1 cut(s) 200
Bsh1236I CGCG 2 cut(s) 23, 1829
BshFI GGCC 2 cut(s) 1371, 1523
BshNI GGYRCC 1 cut(s) 1028
BshTI ACCGGT 1 cut(s) 1716
BshVI ATCGAT 2 cut(s) 252, 915
BsiHKAI GWGCWC 2 cut(s) 10, 235
BsiHKCI CYCGRG 1 cut(s) 944
BsiSI CCGG 2 cut(s) 964, 1717
BsiWI CGTACG 1 cut(s) 900
BslFI GGGAC 4 cut(s) 629, 1265, 1363, 1544
BslI CCNNNNNNNGG 1 cut(s) 1568
BsmAI GTCTC 6 cut(s) 324, 371, 716, 943, 1285, 1771
BsmBI CGTCTC 2 cut(s) 371, 943
BsmFI GGGAC 4 cut(s) 629, 1265, 1363, 1544
BsnI GGCC 2 cut(s) 1371, 1523
Bso31I GGTCTC 1 cut(s) 1285
BsoBI CYCGRG 1 cut(s) 944
Bsp119I TTCGAA 1 cut(s) 1914
Bsp1286I GDGCHC 3 cut(s) 10, 235, 1949
Bsp1407I TGTACA 1 cut(s) 1818
Bsp19I CCATGG 2 cut(s) 1494, 1524
BspACI CCGC 8 cut(s) 21, 23, 26, 612, 924, 1249, 1347, 1685
BspANI GGCC 2 cut(s) 1371, 1523
BspCNI CTCAG 4 cut(s) 22, 691, 1396, 2098
BspDI ATCGAT 2 cut(s) 252, 915
BspFNI CGCG 2 cut(s) 23, 1829
BspHI TCATGA 3 cut(s) 495, 805, 1456
BspLI GGNNCC 2 cut(s) 1030, 1522
BspMAI CTGCAG 4 cut(s) 997, 1212, 1357, 1744
BspMI ACCTGC 2 cut(s) 1273, 1415
BspPI GGATC 6 cut(s) 60, 212, 544, 557, 845, 1099
BspQI GCTCTTC 1 cut(s) 1314
BspT104I TTCGAA 1 cut(s) 1914
BspT107I GGYRCC 1 cut(s) 1028
BspTI CTTAAG 1 cut(s) 1657
BspTNI GGTCTC 1 cut(s) 1285
BsrFI RCCGGY 1 cut(s) 1716
BsrGI TGTACA 1 cut(s) 1818
BsrI ACTGG 3 cut(s) 333, 1522, 1785
BssAI RCCGGY 1 cut(s) 1716
BssECI CCNNGG 3 cut(s) 21, 1494, 1524
BssNI GRCGYC 1 cut(s) 380
BssT1I CCWWGG 2 cut(s) 1494, 1524
Bst2UI CCWGG 1 cut(s) 1215
Bst4CI ACNGT 4 cut(s) 424, 652, 865, 1015
Bst6I CTCTTC 5 cut(s) 204, 1314, 1911, 1932, 2019
BstACI GRCGYC 1 cut(s) 380
BstAFI CTTAAG 1 cut(s) 1657
BstAPI GCANNNNNTGC 1 cut(s) 1748
BstAUI TGTACA 1 cut(s) 1818
BstBAI YACGTR 1 cut(s) 904
BstBI TTCGAA 1 cut(s) 1914
BstDEI CTNAG 8 cut(s) 9, 30, 56, 625, 699, 707, 1404, 2106
BstDSI CCRYGG 3 cut(s) 21, 1494, 1524
BstF5I GGATG 3 cut(s) 159, 1085, 1552
BstFNI CGCG 2 cut(s) 23, 1829
BstH2I RGCGCY 1 cut(s) 1455
BstHHI GCGC 2 cut(s) 747, 1454
BstMAI GTCTC 6 cut(s) 324, 371, 716, 943, 1285, 1771
BstMWI GCNNNNNNNGC 3 cut(s) 930, 974, 1748
BstNI CCWGG 1 cut(s) 1215
BstSCI CCNGG 2 cut(s) 963, 1213
BstSFI CTRYAG 5 cut(s) 993, 1208, 1302, 1353, 1740
BstSNI TACGTA 1 cut(s) 904
BstUI CGCG 2 cut(s) 23, 1829
BstV1I GCAGC 3 cut(s) 817, 1222, 1356
BstV2I GAAGAC 1 cut(s) 1314
BstX2I RGATCY 3 cut(s) 52, 536, 562
BstYI RGATCY 3 cut(s) 52, 536, 562
Bsu15I ATCGAT 2 cut(s) 252, 915
Bsu36I CCTNAGG 1 cut(s) 699
BsuRI GGCC 2 cut(s) 1371, 1523
BsuTUI ATCGAT 2 cut(s) 252, 915
BtgI CCRYGG 3 cut(s) 21, 1494, 1524
BtgZI GCGATG 2 cut(s) 1329, 1844
BtrI CACGTC 1 cut(s) 1990
BtsCI GGATG 3 cut(s) 159, 1085, 1552
BtsI GCAGTG 2 cut(s) 667, 990
BtsIMutI CAGTG 8 cut(s) 340, 667, 861, 990, 1011, 1240, 1515, 1602
BveI ACCTGC 2 cut(s) 1273, 1415
CaiI CAGNNNCTG 1 cut(s) 1202
CciI TCATGA 3 cut(s) 495, 805, 1456
CfoI GCGC 2 cut(s) 747, 1454
Cfr10I RCCGGY 1 cut(s) 1716
Cfr13I GGNCC 2 cut(s) 1412, 1521
Cfr42I CCGCGG 1 cut(s) 24
ClaI ATCGAT 2 cut(s) 252, 915
Csp6I GTAC 9 cut(s) 140, 277, 648, 901, 905, 1262, 1484, 1726, 1819
CspAI ACCGGT 1 cut(s) 1716
CspCI CAANNNNNGTGG 4 cut(s) 319, 354, 1000, 1035
CviAII CATG 7 cut(s) 186, 496, 806, 1457, 1495, 1525, 1732
CviQI GTAC 9 cut(s) 140, 277, 648, 901, 905, 1262, 1484, 1726, 1819
DdeI CTNAG 8 cut(s) 9, 30, 56, 625, 699, 707, 1404, 2106
DraI TTTAAA 1 cut(s) 1875
DrdI GACNNNNNNGTC 2 cut(s) 379, 1556
DseDI GACNNNNNNGTC 2 cut(s) 379, 1556
Eam1104I CTCTTC 5 cut(s) 204, 1314, 1911, 1932, 2019
EarI CTCTTC 5 cut(s) 204, 1314, 1911, 1932, 2019
Eco105I TACGTA 1 cut(s) 904
Eco130I CCWWGG 2 cut(s) 1494, 1524
Eco147I AGGCCT 1 cut(s) 1371
Eco24I GRGCYC 1 cut(s) 1949
Eco31I GGTCTC 1 cut(s) 1285
Eco32I GATATC 1 cut(s) 1513
Eco47I GGWCC 1 cut(s) 1412
Eco47III AGCGCT 1 cut(s) 1453
Eco57I CTGAAG 2 cut(s) 281, 1956
Eco81I CCTNAGG 1 cut(s) 699
Eco88I CYCGRG 1 cut(s) 944
EcoRI GAATTC 1 cut(s) 1652
EcoRII CCWGG 1 cut(s) 1213
EcoRV GATATC 1 cut(s) 1513
EcoT14I CCWWGG 2 cut(s) 1494, 1524
EcoT38I GRGCYC 1 cut(s) 1949
ErhI CCWWGG 2 cut(s) 1494, 1524
Esp3I CGTCTC 2 cut(s) 371, 943
FaeI CATG 7 cut(s) 189, 499, 809, 1460, 1498, 1528, 1735
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FaqI GGGAC 4 cut(s) 629, 1265, 1363, 1544
FatI CATG 7 cut(s) 185, 495, 805, 1456, 1494, 1524, 1731
FauI CCCGC 5 cut(s) 28, 605, 917, 1242, 1340
FauNDI CATATG 2 cut(s) 741, 1121
FbaI TGATCA 1 cut(s) 541
Fnu4HI GCNGC 5 cut(s) 24, 27, 831, 1211, 1345
FokI GGATG 3 cut(s) 146, 1092, 1559
FriOI GRGCYC 1 cut(s) 1949
Fsp4HI GCNGC 5 cut(s) 24, 27, 831, 1211, 1345
FspBI CTAG 4 cut(s) 86, 125, 522, 527
GlaI GCGC 2 cut(s) 746, 1453
GluI GCNGC 5 cut(s) 24, 27, 831, 1211, 1345
GsaI CCCAGC 1 cut(s) 204
GsuI CTGGAG 2 cut(s) 1590, 1773
HaeII RGCGCY 1 cut(s) 1455
HaeIII GGCC 2 cut(s) 1371, 1523
HapII CCGG 2 cut(s) 964, 1717
HhaI GCGC 2 cut(s) 747, 1454
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 7 cut(s) 189, 499, 809, 1460, 1498, 1528, 1735
Hin6I GCGC 2 cut(s) 745, 1452
HinP1I GCGC 2 cut(s) 745, 1452
HincII GTYRAC 1 cut(s) 65
HindII GTYRAC 1 cut(s) 65
HpaII CCGG 2 cut(s) 964, 1717
HphI GGTGA 5 cut(s) 91, 316, 407, 428, 1114
Hpy166II GTNNAC 5 cut(s) 65, 157, 279, 891, 1796
Hpy8I GTNNAC 5 cut(s) 65, 157, 279, 891, 1796
Hpy99I CGWCG 2 cut(s) 244, 385
HpyAV CCTTC 2 cut(s) 1575, 1846
HpyCH4III ACNGT 4 cut(s) 424, 652, 865, 1015
HpyCH4IV ACGT 3 cut(s) 380, 903, 1989
HpyF10VI GCNNNNNNNGC 3 cut(s) 930, 974, 1748
HpyF3I CTNAG 8 cut(s) 9, 30, 56, 625, 699, 707, 1404, 2106
HpySE526I ACGT 3 cut(s) 380, 903, 1989
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 7 cut(s) 189, 499, 809, 1460, 1498, 1528, 1735
HspAI GCGC 2 cut(s) 745, 1452
Ksp22I TGATCA 1 cut(s) 541
KspI CCGCGG 1 cut(s) 24
LguI GCTCTTC 1 cut(s) 1314
LmnI GCTCC 6 cut(s) 852, 1004, 1199, 1421, 1754, 2036
Lsp1109I GCAGC 3 cut(s) 817, 1222, 1356
LweI GCATC 7 cut(s) 334, 964, 1409, 1597, 1876, 1993, 2111
MaeI CTAG 4 cut(s) 86, 125, 522, 527
MaeII ACGT 3 cut(s) 380, 903, 1989
MaeIII GTNAC 6 cut(s) 677, 788, 1132, 1427, 1445, 2048
MflI RGATCY 3 cut(s) 52, 536, 562
MhlI GDGCHC 3 cut(s) 10, 235, 1949
MlyI GAGTC 4 cut(s) 1400, 1730, 1801, 2084
MmeI TCCRAC 3 cut(s) 319, 621, 982
MseI TTAA 7 cut(s) 510, 569, 759, 1599, 1658, 1707, 1874
MslI CAYNNNNRTG 3 cut(s) 164, 500, 1120
MspA1I CMGCKG 4 cut(s) 23, 200, 924, 1347
MspCI CTTAAG 1 cut(s) 1657
MspI CCGG 2 cut(s) 964, 1717
MspR9I CCNGG 2 cut(s) 965, 1215
MvaI CCWGG 1 cut(s) 1215
MvnI CGCG 2 cut(s) 23, 1829
MwoI GCNNNNNNNGC 3 cut(s) 930, 974, 1748
NciI CCSGG 1 cut(s) 965
NcoI CCATGG 2 cut(s) 1494, 1524
NdeI CATATG 2 cut(s) 741, 1121
NlaIII CATG 7 cut(s) 189, 499, 809, 1460, 1498, 1528, 1735
NlaIV GGNNCC 2 cut(s) 1030, 1522
NmuCI GTSAC 2 cut(s) 1445, 2048
NspV TTCGAA 1 cut(s) 1914
PagI TCATGA 3 cut(s) 495, 805, 1456
PceI AGGCCT 1 cut(s) 1371
PciSI GCTCTTC 1 cut(s) 1314
PfeI GAWTC 8 cut(s) 44, 194, 388, 451, 811, 928, 1616, 1931
Pfl23II CGTACG 1 cut(s) 900
PflMI CCANNNNNTGG 1 cut(s) 1568
PinAI ACCGGT 1 cut(s) 1716
PkrI GCNGC 5 cut(s) 25, 28, 832, 1212, 1346
PleI GAGTC 4 cut(s) 1399, 1730, 1800, 2083
PpsI GAGTC 4 cut(s) 1399, 1730, 1800, 2083
Ppu21I YACGTR 1 cut(s) 904
Psp6I CCWGG 1 cut(s) 1213
PspFI CCCAGC 1 cut(s) 200
PspGI CCWGG 1 cut(s) 1213
PspLI CGTACG 1 cut(s) 900
PspN4I GGNNCC 2 cut(s) 1030, 1522
PspPI GGNCC 2 cut(s) 1412, 1521
PstI CTGCAG 4 cut(s) 997, 1212, 1357, 1744
PstNI CAGNNNCTG 1 cut(s) 1202
PsuI RGATCY 3 cut(s) 52, 536, 562
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 9 cut(s) 141, 278, 649, 902, 906, 1263, 1485, 1727, 1820
RsaNI GTAC 9 cut(s) 140, 277, 648, 901, 905, 1262, 1484, 1726, 1819
RseI CAYNNNNRTG 3 cut(s) 164, 500, 1120
SacII CCGCGG 1 cut(s) 24
SapI GCTCTTC 1 cut(s) 1314
SaqAI TTAA 7 cut(s) 510, 569, 759, 1599, 1658, 1707, 1874
SatI GCNGC 5 cut(s) 24, 27, 831, 1211, 1345
Sau96I GGNCC 2 cut(s) 1412, 1521
ScaI AGTACT 1 cut(s) 649
SchI GAGTC 4 cut(s) 1400, 1730, 1801, 2084
ScrFI CCNGG 2 cut(s) 965, 1215
SduI GDGCHC 3 cut(s) 10, 235, 1949
SfaNI GCATC 7 cut(s) 334, 964, 1409, 1597, 1876, 1993, 2111
SfcI CTRYAG 5 cut(s) 993, 1208, 1302, 1353, 1740
Sfr303I CCGCGG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 1914
SgrBI CCGCGG 1 cut(s) 24
SinI GGWCC 1 cut(s) 1412
SmiMI CAYNNNNRTG 3 cut(s) 164, 500, 1120
SmlI CTYRAG 3 cut(s) 1657, 2011, 2093
SmoI CTYRAG 3 cut(s) 1657, 2011, 2093
SnaBI TACGTA 1 cut(s) 904
SseBI AGGCCT 1 cut(s) 1371
SsiI CCGC 8 cut(s) 21, 23, 26, 612, 924, 1249, 1347, 1685
SspMI CTAG 4 cut(s) 86, 125, 522, 527
StuI AGGCCT 1 cut(s) 1371
StyD4I CCNGG 2 cut(s) 963, 1213
StyI CCWWGG 2 cut(s) 1494, 1524
TaaI ACNGT 4 cut(s) 424, 652, 865, 1015
TaiI ACGT 3 cut(s) 383, 906, 1992
TaqI TCGA 8 cut(s) 192, 207, 252, 605, 818, 840, 915, 1914
TatI WGTACW 2 cut(s) 647, 1818
TauI GCSGC 2 cut(s) 26, 29
TfiI GAWTC 8 cut(s) 44, 194, 388, 451, 811, 928, 1616, 1931
Tru1I TTAA 7 cut(s) 510, 569, 759, 1599, 1658, 1707, 1874
Tru9I TTAA 7 cut(s) 510, 569, 759, 1599, 1658, 1707, 1874
TscAI CASTG 8 cut(s) 340, 667, 868, 997, 1018, 1240, 1522, 1609
TseFI GTSAC 2 cut(s) 1445, 2048
TseI GCWGC 3 cut(s) 830, 1210, 1344
Tsp45I GTSAC 2 cut(s) 1445, 2048
TspDTI ATGAA 5 cut(s) 378, 575, 1332, 1922, 2071
TspGWI ACGGA 3 cut(s) 101, 865, 1092
TspRI CASTG 8 cut(s) 340, 667, 868, 997, 1018, 1240, 1522, 1609
Van91I CCANNNNNTGG 1 cut(s) 1568
Vha464I CTTAAG 1 cut(s) 1657
VpaK11BI GGWCC 1 cut(s) 1412
XapI RAATTY 2 cut(s) 1652, 1910
XbaI TCTAGA 1 cut(s) 521
XspI CTAG 4 cut(s) 86, 125, 522, 527
ZraI GACGTC 1 cut(s) 381
ZrmI AGTACT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.