Rh6AG189600

Translation initiation factor eIF-2B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0005877
Physical Location & Seq
Reverse (-)
33930569 .. 33940027
9459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG189600.1

Sequence Viewer

Length: 1872 bp
ATGGGTGCACAGAAGAAAGGCGCGTCTAGGGCTTCAGAGGACCCTGAGGAATTGGCACGTGTCCCACTCCAGGCCATCCTCCTAGCCGATAGTTTCACCACCAAATTCCGACCCATCACTCTTGAACGCCCCAAGGTGCTGTTGCCGTTGGTCAATGCCCCGATGATCAGTTACACGTTGGCGTGGCTTGAGTCTGCTGGCGTTGAGGAGGTTTTTGTTTTCTGCTGTGCTCACTCCAAGCAAGTCATCACTTATTTGGACAATTCTGAGTGGTTTTCCCAACCAAACTTCACCGTCACCACAATTGAGTCACATGATTCTGTTAGTGCCGGAGATGCTCTGCGCTTGATATATGAGCGCAATGTGATACATGGAGATTTTGTCCTAGTCAGTGGGGATACCGTGAGCAACATGTCGCTTACTCAGGTGCTTCAAGAACATAAAGAGAGAAGGAAGAAGGATAGTAATGCTGTAATGACAATTGTTATTAAACGGTCAAAGCCTTCACCAATCACTCGCCAATCTCGACTGGGCACTGATGAGCTCTTTATGGCAGTAGATCCCAACACAAAGCAGCTCTTATATTATGAGGACAAGGCAGACCATGCAAAGGGATCTATATATCTTGATAAGTTATTGCTTGCTGATAAGTCTTCGATTTCTCTGCATAATGACAAACAGGATTGCTATATCGACATCTGCTCTCCTGAAGTCCTCAGCCTTTTCACAGACAATTTTGACTATCAACATTTACGTCGTCATTTTTTAAAGGGATTGCTTCTTGATGATATTATGGGTTACAAAATATTCACACATGAAATTCACTCGACTTATGCGGCTAGGATTGACAACTTCAGAAGCTATGATACAATCAGTAAGGACATAATTCAGAGATGGACTTACCCATTGGTGCCAGATGTTAAGTTTTTCGGAGATTCTTCAACGAAGCTGGAAAGACGGGGGATGTATCGAGCCTCAGATATAGGGCAATCACGATCTGCACAGATTGGTCCTTTTACAGTCATTGGGAATGGCACCAAGATTGGGGACAACACCAAAATTTCAAATTCTGTTATTGGAGAAGGGTGTTCTATTGGACCAAATGTTTCCATAGAAGGTTCTTATATATGGGATAATGTCACCATTGAAGATGGCTGCAAGCTAAGGCATGCTATAGTATGTGATGGAGTGATAATGAAGTCTGGGGCAGTTTTGGAATCGGGTGTAGTTTTGTCTTTCAAGGTTGTGATAGGACAACAATTTATTGTCCCTTCATACTCGAAGGTATCTTTACTTCAACAACCGATAAAGCAAGACAGTGATGAGGAGCTAGAGTATGCTGACAATAGCACTGGGATTGCAGAAATTTCCCCTCCTATACATGCACCGGATATATCGAATGGGGAAATAGCGAACCAACAACAGGAGACTCGGTGTTGGCCTACATCTGAGCTTGGTATTGGTGGGGCTGGTTATGTTTGGTCAGTTTGTGAAGGAGGCCATGATGAAGAGTGGAGGCATTCAGTTGCTCCCATTCCTGCAGAGAAACTTGCTGAGGTAATACAAGCTGCAGAGGATGATCCGGAGTTAGATCCAGATGGCAGCAGTCTCCCACCTTCAGGAGAGCTGAAACTTGATTCTGATTCTGATGAAGAGTATAGAGATGGTTCCTACTTTGAAAATGAGGTTGAAGCAACCTTCCTAAGAGCTGTGGATGAAAATATTAAAGTAGAGAATGTCGTCTTAGAAGTGAACTCACTGAGGTATTCATTTGGTTTTCTCTTGCAGATCTATGGAATACCATGTAACGTGACTATCATTATTATTTTGTGTTTGTGTAATAACTTATCTTTTGAAAGTTCTTTTAATTAA

Protein Analysis

623

Amino Acids

69.34

Weight (kDa)

4.82

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 25 - 165 4.4e-12 Nucleotidyl transferase
LbH_EIF2B PF25084 319 - 422 2.1e-39 EIF2B subunit epsilon LbH domain
GMPPB_C PF25087 332 - 416 3.6e-10 GMPPB C-terminal domain
Hexapep PF00132 340 - 373 1.1e-07 Bacterial transferase hexapeptide (six repeats)
Hexapep_GlmU PF24894 341 - 404 7.2e-06 GlmU-like, C-terminal hexapeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 910, 1034
AccII CGCG 1 cut(s) 23
AccIII TCCGGA 1 cut(s) 1582
AciI CCGC 1 cut(s) 836
AclWI GGATC 4 cut(s) 554, 622, 1574, 1586
AcsI RAATTY 5 cut(s) 104, 819, 1059, 1066, 1365
AcuI CTGAAG 4 cut(s) 18, 729, 838, 1602
AcvI CACGTG 1 cut(s) 59
AfiI CCNNNNNNNGG 5 cut(s) 70, 610, 1044, 1423, 1619
AflIII ACRYGT 3 cut(s) 58, 174, 411
AjnI CCWGG 1 cut(s) 69
Alw21I GWGCWC 3 cut(s) 10, 232, 546
Alw26I GTCTC 2 cut(s) 1421, 1613
Alw44I GTGCAC 1 cut(s) 6
AlwI GGATC 4 cut(s) 554, 622, 1574, 1586
Aor13HI TCCGGA 1 cut(s) 1582
AoxI GGCC 3 cut(s) 72, 1439, 1498
ApaLI GTGCAC 1 cut(s) 6
ApeKI GCWGC 4 cut(s) 574, 1155, 1568, 1602
ApoI RAATTY 5 cut(s) 104, 819, 1059, 1066, 1365
AspLEI GCGC 3 cut(s) 23, 345, 360
AspS9I GGNCC 3 cut(s) 40, 1010, 1097
AsuHPI GGTGA 5 cut(s) 88, 283, 289, 498, 1132
AvaII GGWCC 3 cut(s) 40, 1010, 1097
AxyI CCTNAGG 1 cut(s) 45
BaeGI GKGCMC 2 cut(s) 10, 536
BanI GGYRCC 2 cut(s) 910, 1034
BanII GRGCYC 1 cut(s) 546
BarI GAAGNNNNNNTAC 4 cut(s) 850, 882, 1278, 1310
BbrPI CACGTG 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 645
Bbv12I GWGCWC 3 cut(s) 10, 232, 546
BbvCI CCTCAGC 2 cut(s) 716, 1554
BbvI GCAGC 4 cut(s) 586, 1142, 1555, 1614
BccI CCATC 7 cut(s) 83, 122, 888, 1145, 1178, 1592, 1658
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 2 cut(s) 646, 680
BciT130I CCWGG 1 cut(s) 71
BciVI GTATCC 1 cut(s) 391
BclI TGATCA 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 1421, 1613
BfaI CTAG 5 cut(s) 27, 83, 386, 840, 1331
BfmI CTRYAG 3 cut(s) 1173, 1539, 1569
BfuI GTATCC 1 cut(s) 391
BglII AGATCT 1 cut(s) 1788
BisI GCNGC 5 cut(s) 575, 837, 1156, 1569, 1603
BlsI GCNGC 5 cut(s) 576, 838, 1157, 1570, 1604
Bme1390I CCNGG 1 cut(s) 71
Bme18I GGWCC 3 cut(s) 40, 1010, 1097
BmgT120I GGNCC 3 cut(s) 40, 1010, 1097
BmiI GGNNCC 4 cut(s) 42, 912, 1036, 1669
BmrFI CCNGG 1 cut(s) 71
BmrI ACTGGG 2 cut(s) 539, 1362
BmsI GCATC 1 cut(s) 325
BmuI ACTGGG 2 cut(s) 539, 1362
BpiI GAAGAC 1 cut(s) 645
BpmI CTGGAG 1 cut(s) 53
Bpu10I CCTNAGC 3 cut(s) 716, 1163, 1554
BpuEI CTTGAG 1 cut(s) 209
BsaAI YACGTR 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 132
BsaWI WCCGGW 2 cut(s) 1387, 1582
BsaXI ACNNNNNCTCC 6 cut(s) 366, 396, 1319, 1349, 1418, 1448
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 610, 1044, 1423, 1619
Bse1I ACTGG 2 cut(s) 534, 1357
Bse21I CCTNAGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 367
BseAI TCCGGA 1 cut(s) 1582
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 4 cut(s) 75, 969, 1582, 1720
BseLI CCNNNNNNNGG 5 cut(s) 70, 610, 1044, 1423, 1619
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 8 cut(s) 36, 258, 437, 730, 990, 1440, 1545, 1751
BseNI ACTGG 2 cut(s) 534, 1357
BseRI GAGGAG 2 cut(s) 221, 1340
BseSI GKGCMC 2 cut(s) 10, 536
BseXI GCAGC 4 cut(s) 586, 1142, 1555, 1614
BsgI GTGCAG 1 cut(s) 984
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 74, 1441, 1500
BshNI GGYRCC 2 cut(s) 910, 1034
BsiHKAI GWGCWC 3 cut(s) 10, 232, 546
BsiSI CCGG 3 cut(s) 330, 1388, 1583
BslFI GGGAC 3 cut(s) 47, 1061, 1253
BslI CCNNNNNNNGG 5 cut(s) 70, 610, 1044, 1423, 1619
BsmAI GTCTC 2 cut(s) 1421, 1613
BsmFI GGGAC 3 cut(s) 47, 1061, 1253
BsmI GAATGC 1 cut(s) 1519
BsnI GGCC 3 cut(s) 74, 1441, 1500
Bsp1286I GDGCHC 4 cut(s) 10, 232, 536, 546
Bsp13I TCCGGA 1 cut(s) 1582
Bsp143I GATC 7 cut(s) 165, 559, 614, 995, 1579, 1591, 1788
BspACI CCGC 1 cut(s) 836
BspANI GGCC 3 cut(s) 74, 1441, 1500
BspCNI CTCAG 8 cut(s) 37, 259, 436, 729, 989, 1441, 1546, 1752
BspEI TCCGGA 1 cut(s) 1582
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 4 cut(s) 42, 912, 1036, 1669
BspMAI CTGCAG 2 cut(s) 1543, 1573
BspPI GGATC 4 cut(s) 554, 622, 1574, 1586
BspT107I GGYRCC 2 cut(s) 910, 1034
BsrDI GCAATG 1 cut(s) 367
BsrI ACTGG 2 cut(s) 534, 1357
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 7 cut(s) 165, 559, 614, 995, 1579, 1591, 1788
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 5 cut(s) 295, 403, 495, 1021, 1319
Bst6I CTCTTC 2 cut(s) 1503, 1647
BstAPI GCANNNNNTGC 1 cut(s) 605
BstBAI YACGTR 1 cut(s) 59
BstC8I GCNNGC 4 cut(s) 199, 642, 1160, 1170
BstF5I GGATG 4 cut(s) 75, 969, 1582, 1720
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 3 cut(s) 23, 345, 360
BstKTI GATC 7 cut(s) 168, 562, 617, 998, 1582, 1594, 1791
BstMAI GTCTC 2 cut(s) 1421, 1613
BstMBI GATC 7 cut(s) 165, 559, 614, 995, 1579, 1591, 1788
BstMWI GCNNNNNNNGC 3 cut(s) 29, 335, 605
BstNI CCWGG 1 cut(s) 71
BstNSI RCATGY 3 cut(s) 415, 1172, 1385
BstSCI CCNGG 1 cut(s) 69
BstSFI CTRYAG 3 cut(s) 1173, 1539, 1569
BstSLI GKGCMC 2 cut(s) 10, 536
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 4 cut(s) 586, 1142, 1555, 1614
BstV2I GAAGAC 1 cut(s) 645
BstX2I RGATCY 4 cut(s) 559, 614, 1591, 1788
BstYI RGATCY 4 cut(s) 559, 614, 1591, 1788
Bsu36I CCTNAGG 1 cut(s) 45
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 3 cut(s) 74, 1441, 1500
BtsCI GGATG 4 cut(s) 75, 969, 1582, 1720
BtsIMutI CAGTG 5 cut(s) 397, 534, 1324, 1350, 1757
Cac8I GCNNGC 4 cut(s) 199, 642, 1160, 1170
CfoI GCGC 3 cut(s) 23, 345, 360
Cfr13I GGNCC 3 cut(s) 40, 1010, 1097
CseI GACGC 1 cut(s) 12
CviAII CATG 9 cut(s) 314, 371, 412, 605, 815, 1169, 1382, 1502, 1803
DpnI GATC 7 cut(s) 167, 561, 616, 997, 1581, 1593, 1790
DpnII GATC 7 cut(s) 165, 559, 614, 995, 1579, 1591, 1788
DraI TTTAAA 1 cut(s) 768
Eam1104I CTCTTC 2 cut(s) 1503, 1647
EarI CTCTTC 2 cut(s) 1503, 1647
Ecl136II GAGCTC 1 cut(s) 544
Eco130I CCWWGG 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 546
Eco47I GGWCC 3 cut(s) 40, 1010, 1097
Eco53kI GAGCTC 1 cut(s) 544
Eco57I CTGAAG 4 cut(s) 18, 729, 838, 1602
Eco72I CACGTG 1 cut(s) 59
Eco81I CCTNAGG 1 cut(s) 45
EcoICRI GAGCTC 1 cut(s) 544
EcoO109I RGGNCCY 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 546
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 9 cut(s) 317, 374, 415, 608, 818, 1172, 1385, 1505, 1806
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FaqI GGGAC 3 cut(s) 47, 1061, 1253
FatI CATG 9 cut(s) 313, 370, 411, 604, 814, 1168, 1381, 1501, 1802
FbaI TGATCA 1 cut(s) 165
Fnu4HI GCNGC 5 cut(s) 575, 837, 1156, 1569, 1603
FokI GGATG 4 cut(s) 62, 976, 1589, 1727
FriOI GRGCYC 1 cut(s) 546
Fsp4HI GCNGC 5 cut(s) 575, 837, 1156, 1569, 1603
FspBI CTAG 5 cut(s) 27, 83, 386, 840, 1331
GlaI GCGC 3 cut(s) 22, 344, 359
GluI GCNGC 5 cut(s) 575, 837, 1156, 1569, 1603
GsuI CTGGAG 1 cut(s) 53
HaeIII GGCC 3 cut(s) 74, 1441, 1500
HapII CCGG 3 cut(s) 330, 1388, 1583
HgaI GACGC 1 cut(s) 12
HhaI GCGC 3 cut(s) 23, 345, 360
Hin1II CATG 9 cut(s) 317, 374, 415, 608, 818, 1172, 1385, 1505, 1806
Hin6I GCGC 3 cut(s) 21, 343, 358
HinP1I GCGC 3 cut(s) 21, 343, 358
HinfI GANTC 8 cut(s) 191, 308, 317, 935, 1217, 1429, 1637, 1643
HpaII CCGG 3 cut(s) 330, 1388, 1583
HphI GGTGA 5 cut(s) 88, 283, 289, 498, 1132
Hpy166II GTNNAC 2 cut(s) 8, 1753
Hpy8I GTNNAC 2 cut(s) 8, 1753
Hpy99I CGWCG 1 cut(s) 759
HpyCH4III ACNGT 5 cut(s) 295, 403, 495, 1021, 1319
HpyCH4IV ACGT 4 cut(s) 58, 176, 754, 1809
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 335, 605
HpySE526I ACGT 4 cut(s) 58, 176, 754, 1809
Hsp92II CATG 9 cut(s) 317, 374, 415, 608, 818, 1172, 1385, 1505, 1806
HspAI GCGC 3 cut(s) 21, 343, 358
Kpn2I TCCGGA 1 cut(s) 1582
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 7 cut(s) 165, 559, 614, 995, 1579, 1591, 1788
LmnI GCTCC 2 cut(s) 1327, 1534
Lsp1109I GCAGC 4 cut(s) 586, 1142, 1555, 1614
LweI GCATC 1 cut(s) 325
MaeI CTAG 5 cut(s) 27, 83, 386, 840, 1331
MaeII ACGT 4 cut(s) 58, 176, 754, 1809
MaeIII GTNAC 7 cut(s) 170, 295, 309, 797, 1138, 1805, 1810
MalI GATC 7 cut(s) 167, 561, 616, 997, 1581, 1593, 1790
MboI GATC 7 cut(s) 165, 559, 614, 995, 1579, 1591, 1788
MboII GAAGA 7 cut(s) 25, 466, 645, 930, 1160, 1520, 1664
MfeI CAATTG 2 cut(s) 303, 480
MflI RGATCY 4 cut(s) 559, 614, 1591, 1788
MhlI GDGCHC 4 cut(s) 10, 232, 536, 546
MlyI GAGTC 3 cut(s) 200, 317, 1423
MmeI TCCRAC 1 cut(s) 133
MroI TCCGGA 1 cut(s) 1582
MseI TTAA 6 cut(s) 489, 767, 921, 1725, 1866, 1870
MspI CCGG 3 cut(s) 330, 1388, 1583
MspR9I CCNGG 1 cut(s) 71
MunI CAATTG 2 cut(s) 303, 480
Mva1269I GAATGC 1 cut(s) 1519
MvaI CCWGG 1 cut(s) 71
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 29, 335, 605
NdeII GATC 7 cut(s) 165, 559, 614, 995, 1579, 1591, 1788
NlaIII CATG 9 cut(s) 317, 374, 415, 608, 818, 1172, 1385, 1505, 1806
NlaIV GGNNCC 4 cut(s) 42, 912, 1036, 1669
NmuCI GTSAC 4 cut(s) 295, 309, 1138, 1810
NspI RCATGY 3 cut(s) 415, 1172, 1385
PacI TTAATTAA 1 cut(s) 1870
PaeI GCATGC 1 cut(s) 1172
PciI ACATGT 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 523
PctI GAATGC 1 cut(s) 1519
PfeI GAWTC 5 cut(s) 317, 935, 1217, 1637, 1643
PkrI GCNGC 5 cut(s) 576, 838, 1157, 1570, 1604
PleI GAGTC 3 cut(s) 199, 316, 1423
PmaCI CACGTG 1 cut(s) 59
PmlI CACGTG 1 cut(s) 59
PpsI GAGTC 3 cut(s) 199, 316, 1423
Ppu21I YACGTR 1 cut(s) 59
PpuMI RGGWCCY 1 cut(s) 40
PscI ACATGT 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 546
Psp5II RGGWCCY 1 cut(s) 40
Psp6I CCWGG 1 cut(s) 69
PspCI CACGTG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 4 cut(s) 42, 912, 1036, 1669
PspPI GGNCC 3 cut(s) 40, 1010, 1097
PspPPI RGGWCCY 1 cut(s) 40
PstI CTGCAG 2 cut(s) 1543, 1573
PsuI RGATCY 4 cut(s) 559, 614, 1591, 1788
SacI GAGCTC 1 cut(s) 546
SaqAI TTAA 6 cut(s) 489, 767, 921, 1725, 1866, 1870
SatI GCNGC 5 cut(s) 575, 837, 1156, 1569, 1603
Sau3AI GATC 7 cut(s) 165, 559, 614, 995, 1579, 1591, 1788
Sau96I GGNCC 3 cut(s) 40, 1010, 1097
SchI GAGTC 3 cut(s) 200, 317, 1423
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 4 cut(s) 10, 232, 536, 546
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 3 cut(s) 1173, 1539, 1569
SinI GGWCC 3 cut(s) 40, 1010, 1097
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
SphI GCATGC 1 cut(s) 1172
SsiI CCGC 1 cut(s) 836
SspI AATATT 2 cut(s) 807, 1723
SspMI CTAG 5 cut(s) 27, 83, 386, 840, 1331
SstI GAGCTC 1 cut(s) 546
StyD4I CCNGG 1 cut(s) 69
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 5 cut(s) 295, 403, 495, 1021, 1319
TaiI ACGT 4 cut(s) 61, 179, 757, 1812
TaqI TCGA 7 cut(s) 526, 656, 693, 827, 970, 1280, 1397
TauI GCSGC 1 cut(s) 839
TfiI GAWTC 5 cut(s) 317, 935, 1217, 1637, 1643
Tru1I TTAA 6 cut(s) 489, 767, 921, 1725, 1866, 1870
Tru9I TTAA 6 cut(s) 489, 767, 921, 1725, 1866, 1870
TscAI CASTG 5 cut(s) 397, 541, 1324, 1357, 1764
TseFI GTSAC 4 cut(s) 295, 309, 1138, 1810
TseI GCWGC 4 cut(s) 574, 1155, 1568, 1602
Tsp45I GTSAC 4 cut(s) 295, 309, 1138, 1810
TspDTI ATGAA 7 cut(s) 831, 1211, 1263, 1521, 1665, 1731, 1758
TspRI CASTG 5 cut(s) 397, 541, 1324, 1357, 1764
VneI GTGCAC 1 cut(s) 6
VpaK11BI GGWCC 3 cut(s) 40, 1010, 1097
XapI RAATTY 5 cut(s) 104, 819, 1059, 1066, 1365
XceI RCATGY 3 cut(s) 415, 1172, 1385
XspI CTAG 5 cut(s) 27, 83, 386, 840, 1331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.