Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005877

Physical Location & Seq

Genomic Coordinates Forward (+)

30727855 .. 30739302

Gene Structure 11448 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1232500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2202 bp

ATGGGCGCACAGAAAAGAGGTGCACCCAGGGTTTCAGAGGACCCTGAGGAACTGGCACGTGTCCCCTTGCAGGCCATCCTCTTAGCTGATAGTTTCACCACCAAATTCCGACCCATCACTCTCGAGCGCCCCAAAGTGCTGTTGCCATTGGTAAATGTCCCGATGATCAATTACACGCTAGCATGGCTTGAGTCCGCTGGTGTCGAGGAGGTTTTTGTTTTTTGTTGTGCTCATTCTAAGCAAGTGATTGATTATTTGGAGAGTTCAGAGTGGTTTGCTCAACCAAACTTCACAGTCACAACGATTGAATCACATAATTCTGTTAGTGCTGGAGATGCTCTGCGCTTGATATATGAGCGCAATGTGATACATGGAGATTTTGTTCTAATTAGTGGGGACACTCTGAGCAACATGTCGCTTACTCAGGTACTTCAAGAACATAAAGAGAGAAGAAAAAAAGATAGTAATGCTGTGATGACAATAGTTATAAAACGGTCAAAGCCTTCTCAGATCACTCATCAATCTCGACTGGGCAATGATGAGCTGTTTATGGCAACAGATCCTAATACAAAGCAACTTTTATATTATGAGGACAAGGAAGACCATACAAAAGGATCCATCTATCTTGACAAGTTGTTGATTGCTGATAACCCTTCAATTACTTTACATAATGACAAACAGGATTGCTATATTGATATATGCTCTCCTGAAGTCCTTAGCCTTTTCACAGACAATTTTGATTATCAACATCTACGTCATTTTGTGAAGGGATTGCTTGTTGATGATATTATGGGTTACAAAATATTCACACATGAAATTCACTCGAGTTATGCGGCTAGGATTGACAACTTCCGAAGCTATGATACAGTTAGTAAGGACATAATTCAGAGATGGACTTACCCATTGGTGCCAGATGTGAAGTTTTTTGGGAATTCTTCGATAAAACTGGAAAGACAGGGGATGTATCGAGCATCAGACACAGGGCAATCGCGCTCTGCACAGATTGGGCCTTTTACAGTCATTGGGAGTGGCACCAAAATTGGGAACAACACCAAAATTTCAAATTCTGTTATTGGGGAAGGGTGCTCTATTGGATCAAATGTGTCAATAGAAGGCTCCTATATATGGGATAATGCCACTATTGAAGATGGCTGCAAGCTGAGGCATGCTATTGTATGTGATGGAGTGATCATGAGGTCTGGAGCAGTTTTGGAACCTGGTGTAGTTTTGTCTTTCAAGGTTGTTATAGGACAAAAATTTAATGTCCTTTCATACTCAAAGGTATCTCTACTTCAGCAGCCAACTAAGCTAGATAGTGACGACGAGCTGGAGTATGCTGACAATAGCAGCGGGATTGCAGAAATGTCATCTATCACACCTGTTGATAAACTAAATGAGGAAACAACGAACCAACGAATGGAGGCTAACTGTTGGCCCACATCTGAGCTTGGTACTGGTGGGGCTGGTTATGTTTGGTCAATTTGTGAAGGAGGCCACGATGAGGAGTGGAGGCATTCAGTTGCTCCCATTCCTGCTGACAAACTTTTGGAGGCAATACATGCTGCAGATGATGATTTGGAGCATGTTCAAGATGGCAGCTCCCTCCCACCTTCAGGAGAGTTAAAACCTGATTCTAATGATTCTGAAGGCGACGATGTAGTGTCTATAGAGGATTCTGCCGACTTTGAGAGAGAGGTTGAAGCAACCTTTTTAAGAGCTGTGCATGAAAACATACAAGTAGACCACGTCATATTAGAAGTGAATTCACTGAGGTTGTCATACAACAAGGTAGCTGCAGACTGTGCTGGAGCTTTATTTTATTCAATGATGAAATTGGCATTAGAAACTCCATATGGTTCACCAGGTGAATTGGTTCAAGCTACTGTTAACATATTTACAAAATGGGAAAAAGTGCTGAAGTCTTACCTAGCAGAAATAGACGAGGAGATTGAAGTGATACTTAGATTTGAAGAAATGTGTGAGTCCCTTAAGGAATTCTCTCCTGTGTTTGACAAGATTTTGCATCAACTGTACGAACTAGAAATTATACAAGAAGAAGCCATTATGAGGTGGGACGCCGAAAAGAAAAATGCTGAGGAAGCAGATAGAGTTTATGTCAAGCAATCAGCAGACTTTATTCAATGGTTGAGAGAAGCCGAAGAAGAAGATGAAGATGAAGAAGATGAAGAAGATGAAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0005085 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005851 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0019538 GO:0019899 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0051020 GO:0065007 GO:0065009 GO:0071704 GO:0097159 GO:0098772 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Hexapep Hexapep_2 NTP_transferase W2

Protein Analysis

734

Amino Acids

82.45

Weight (kDa)

4.62

Isoelectric Point (pI)

45.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NTP_transferase	PF00483	25 - 166	8.7e-12	Nucleotidyl transferase
LbH_EIF2B	PF25084	318 - 422	4.8e-39	EIF2B subunit epsilon LbH domain
GMPPB_C	PF25087	331 - 415	1.1e-10	GMPPB C-terminal domain
Hexapep	PF00132	339 - 372	7.4e-07	Bacterial transferase hexapeptide (six repeats)
Hexapep_GlmU	PF24894	341 - 403	8.3e-06	GlmU-like, C-terminal hexapeptide
W2	PF02020	648 - 726	1.5e-18	eIF4-gamma/eIF5/eIF2-epsilon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005877)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34970 AT4G18300
fragaria_vesca	FvH4_2g12670 FvH4_2g12670
malus_domestica	MD00G1051200.v1.1 MD12G1232500.v1.1 MD17G1228000.v1.1
prunus_persica	Prupe.6G335900_v2.0.a1
pyrus_communis	pycom04g19200 pycom12g21490
rosa_chinensis	RchiOBHm_Chr2g0129391 RchiOBHm_Chr6g0273391
rosa_laevigata	RLG00000013621 RLG00000019076
rosa_multiflora	Rmu_sc0001171.1_g000012 Rmu_sc0005891.1_g000011
rosa_roxburghii	Rroxscaffold_2G00114650 Rroxscaffold_7G00195420
rosa_rugosa	Rorug06G0074500
rosa_samantha	Rh2AG338500 Rh2BG346600 Rh2CG326000 Rh2DG364400 Rh6AG189600 Rh6BG193000 Rh6CG192800 Rh6DG184300
rosa_wichuraiana	Rw6G016500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	488
AccB1I	GGYRCC	2 cut(s)	907, 1031
AccB7I	CCANNNNNTGG	1 cut(s)	1417
AccI	GTMKAC	1 cut(s)	1740
AccII	CGCG	1 cut(s)	991
AciI	CCGC	3 cut(s)	195, 833, 1350
AclWI	GGATC	4 cut(s)	554, 609, 622, 1102
AcsI	RAATTY	8 cut(s)	104, 816, 931, 1056, 1063, 1256, 1762, 1994
AcuI	CTGAAG	5 cut(s)	729, 1277, 1596, 1665, 1937
AcvI	CACGTG	1 cut(s)	59
AcyI	GRCGYC	1 cut(s)	2076
AdeI	CACNNNGTG	1 cut(s)	1865
AfaI	GTAC	3 cut(s)	429, 1453, 2033
AfiI	CCNNNNNNNGG	7 cut(s)	70, 1041, 1125, 1417, 1501, 1613, 2067
AflII	CTTAAG	1 cut(s)	1988
AflIII	ACRYGT	2 cut(s)	58, 411
AjiI	CACGTC	1 cut(s)	1747
AjnI	CCWGG	3 cut(s)	26, 1216, 1861
AloI	GAACNNNNNNTCC	2 cut(s)	366, 398
Alw21I	GWGCWC	3 cut(s)	25, 232, 1088
Alw44I	GTGCAC	1 cut(s)	21
AlwI	GGATC	4 cut(s)	554, 609, 622, 1102
Ama87I	CYCGRG	2 cut(s)	122, 823
AoxI	GGCC	4 cut(s)	72, 1007, 1433, 1492
ApaLI	GTGCAC	1 cut(s)	21
ApeKI	GCWGC	6 cut(s)	1152, 1297, 1347, 1562, 1596, 1793
ApoI	RAATTY	8 cut(s)	104, 816, 931, 1056, 1063, 1256, 1762, 1994
Asp700I	GAANNNNTTC	1 cut(s)	1872
AspLEI	GCGC	5 cut(s)	8, 129, 345, 360, 993
AspS9I	GGNCC	3 cut(s)	40, 1007, 1434
AsuHPI	GGTGA	3 cut(s)	88, 1851, 1877
AsuNHI	GCTAGC	1 cut(s)	178
AvaI	CYCGRG	2 cut(s)	122, 823
AvaII	GGWCC	1 cut(s)	40
AxyI	CCTNAGG	1 cut(s)	45
BaeGI	GKGCMC	1 cut(s)	25
BamHI	GGATCC	1 cut(s)	614
BanI	GGYRCC	2 cut(s)	907, 1031
BarI	GAAGNNNNNNTAC	4 cut(s)	847, 879, 1275, 1307
BbrPI	CACGTG	1 cut(s)	59
BbsI	GAAGAC	1 cut(s)	606
Bbv12I	GWGCWC	3 cut(s)	25, 232, 1088
BbvCI	CCTCAGC	2 cut(s)	1160, 2094
BbvI	GCAGC	6 cut(s)	1139, 1309, 1359, 1549, 1608, 1780
BccI	CCATC	7 cut(s)	83, 122, 626, 885, 1142, 1175, 1586
BciT130I	CCWGG	3 cut(s)	28, 1218, 1863
BclI	TGATCA	2 cut(s)	165, 1188
BfaI	CTAG	5 cut(s)	179, 837, 1310, 1928, 2039
BfmI	CTRYAG	3 cut(s)	1563, 1665, 1794
BfoI	RGCGCY	1 cut(s)	130
BfrI	CTTAAG	1 cut(s)	1988
BisI	GCNGC	7 cut(s)	834, 1153, 1298, 1348, 1563, 1597, 1794
BlsI	GCNGC	7 cut(s)	835, 1154, 1299, 1349, 1564, 1598, 1795
Bme1390I	CCNGG	3 cut(s)	28, 1218, 1863
Bme18I	GGWCC	1 cut(s)	40
BmeT110I	CYCGRG	2 cut(s)	122, 823
BmgBI	CACGTC	1 cut(s)	1747
BmgT120I	GGNCC	3 cut(s)	40, 1007, 1434
BmiI	GGNNCC	6 cut(s)	42, 616, 909, 1033, 1117, 1215
BmrFI	CCNGG	3 cut(s)	28, 1218, 1863
BmrI	ACTGGG	1 cut(s)	539
BmsI	GCATC	3 cut(s)	325, 980, 2032
BmtI	GCTAGC	1 cut(s)	182
BmuI	ACTGGG	1 cut(s)	539
BpiI	GAAGAC	1 cut(s)	606
BpmI	CTGGAG	4 cut(s)	351, 1221, 1349, 1827
Bpu10I	CCTNAGC	3 cut(s)	716, 1160, 2094
BpuEI	CTTGAG	1 cut(s)	209
BsaAI	YACGTR	1 cut(s)	59
BsaHI	GRCGYC	1 cut(s)	2076
BsaJI	CCNNGG	2 cut(s)	26, 27
BsaXI	ACNNNNNCTCC	2 cut(s)	366, 396
Bsc4I	CCNNNNNNNGG	7 cut(s)	70, 1041, 1125, 1417, 1501, 1613, 2067
Bse1I	ACTGG	4 cut(s)	57, 534, 951, 1459
Bse21I	CCTNAGG	1 cut(s)	45
Bse3DI	GCAATG	2 cut(s)	367, 541
BseBI	CCWGG	3 cut(s)	28, 1218, 1863
BseDI	CCNNGG	2 cut(s)	26, 27
BseGI	GGATG	2 cut(s)	75, 966
BseLI	CCNNNNNNNGG	7 cut(s)	70, 1041, 1125, 1417, 1501, 1613, 2067
BseMI	GCAATG	2 cut(s)	367, 541
BseMII	CTCAG	8 cut(s)	36, 395, 437, 521, 1151, 1434, 1760, 2085
BseNI	ACTGG	4 cut(s)	57, 534, 951, 1459
BseRI	GAGGAG	3 cut(s)	221, 1517, 1958
BseSI	GKGCMC	1 cut(s)	25
BseXI	GCAGC	6 cut(s)	1139, 1309, 1359, 1549, 1608, 1780
BsgI	GTGCAG	1 cut(s)	981
Bsh1236I	CGCG	1 cut(s)	991
BshFI	GGCC	4 cut(s)	74, 1009, 1435, 1494
BshNI	GGYRCC	2 cut(s)	907, 1031
BsiHKAI	GWGCWC	3 cut(s)	25, 232, 1088
BsiHKCI	CYCGRG	2 cut(s)	122, 823
BslFI	GGGAC	5 cut(s)	47, 143, 410, 1969, 2087
BslI	CCNNNNNNNGG	7 cut(s)	70, 1041, 1125, 1417, 1501, 1613, 2067
BsmFI	GGGAC	5 cut(s)	47, 143, 410, 1969, 2087
BsmI	GAATGC	1 cut(s)	1513
BsnI	GGCC	4 cut(s)	74, 1009, 1435, 1494
BsoBI	CYCGRG	2 cut(s)	122, 823
Bsp1286I	GDGCHC	3 cut(s)	25, 232, 1088
Bsp143I	GATC	6 cut(s)	165, 510, 559, 614, 1094, 1188
BspACI	CCGC	3 cut(s)	195, 833, 1350
BspANI	GGCC	4 cut(s)	74, 1009, 1435, 1494
BspCNI	CTCAG	8 cut(s)	37, 396, 436, 520, 1152, 1435, 1761, 2086
BspFNI	CGCG	1 cut(s)	991
BspHI	TCATGA	1 cut(s)	1191
BspLI	GGNNCC	6 cut(s)	42, 616, 909, 1033, 1117, 1215
BspMAI	CTGCAG	2 cut(s)	1567, 1798
BspOI	GCTAGC	1 cut(s)	182
BspPI	GGATC	4 cut(s)	554, 609, 622, 1102
BspT107I	GGYRCC	2 cut(s)	907, 1031
BspTI	CTTAAG	1 cut(s)	1988
BsrDI	GCAATG	2 cut(s)	367, 541
BsrI	ACTGG	4 cut(s)	57, 534, 951, 1459
BssECI	CCNNGG	2 cut(s)	26, 27
BssMI	GATC	6 cut(s)	165, 510, 559, 614, 1094, 1188
BssNI	GRCGYC	1 cut(s)	2076
Bst2UI	CCWGG	3 cut(s)	28, 1218, 1863
Bst4CI	ACNGT	8 cut(s)	295, 495, 868, 1018, 1430, 1802, 1885, 2031
BstACI	GRCGYC	1 cut(s)	2076
BstAFI	CTTAAG	1 cut(s)	1988
BstAPI	GCANNNNNTGC	2 cut(s)	1559, 1802
BstBAI	YACGTR	1 cut(s)	59
BstC8I	GCNNGC	4 cut(s)	72, 180, 1157, 1167
BstF5I	GGATG	2 cut(s)	75, 966
BstFNI	CGCG	1 cut(s)	991
BstH2I	RGCGCY	1 cut(s)	130
BstHHI	GCGC	5 cut(s)	8, 129, 345, 360, 993
BstKTI	GATC	6 cut(s)	168, 513, 562, 617, 1097, 1191
BstMBI	GATC	6 cut(s)	165, 510, 559, 614, 1094, 1188
BstMWI	GCNNNNNNNGC	6 cut(s)	184, 335, 1306, 1559, 1802, 2099
BstNI	CCWGG	3 cut(s)	28, 1218, 1863
BstNSI	RCATGY	4 cut(s)	415, 1169, 1562, 1586
BstSCI	CCNGG	3 cut(s)	26, 1216, 1861
BstSFI	CTRYAG	3 cut(s)	1563, 1665, 1794
BstSLI	GKGCMC	1 cut(s)	25
BstUI	CGCG	1 cut(s)	991
BstV1I	GCAGC	6 cut(s)	1139, 1309, 1359, 1549, 1608, 1780
BstV2I	GAAGAC	1 cut(s)	606
BstX2I	RGATCY	2 cut(s)	559, 614
BstYI	RGATCY	2 cut(s)	559, 614
Bsu36I	CCTNAGG	1 cut(s)	45
BsuRI	GGCC	4 cut(s)	74, 1009, 1435, 1494
BtrI	CACGTC	1 cut(s)	1747
BtsCI	GGATG	2 cut(s)	75, 966
BtsIMutI	CAGTG	1 cut(s)	1766
Cac8I	GCNNGC	4 cut(s)	72, 180, 1157, 1167
CciI	TCATGA	1 cut(s)	1191
CfoI	GCGC	5 cut(s)	8, 129, 345, 360, 993
Cfr13I	GGNCC	3 cut(s)	40, 1007, 1434
CseI	GACGC	1 cut(s)	2084
CsiI	ACCWGGT	2 cut(s)	1216, 1861
Csp6I	GTAC	3 cut(s)	428, 1452, 2032
CviAII	CATG	9 cut(s)	183, 371, 412, 812, 1166, 1192, 1559, 1583, 1724
CviQI	GTAC	3 cut(s)	428, 1452, 2032
DpnI	GATC	6 cut(s)	167, 512, 561, 616, 1096, 1190
DpnII	GATC	6 cut(s)	165, 510, 559, 614, 1094, 1188
DraIII	CACNNNGTG	1 cut(s)	1865
Eco47I	GGWCC	1 cut(s)	40
Eco57I	CTGAAG	5 cut(s)	729, 1277, 1596, 1665, 1937
Eco72I	CACGTG	1 cut(s)	59
Eco81I	CCTNAGG	1 cut(s)	45
Eco88I	CYCGRG	2 cut(s)	122, 823
EcoO109I	RGGNCCY	1 cut(s)	40
EcoRI	GAATTC	3 cut(s)	931, 1762, 1994
EcoRII	CCWGG	3 cut(s)	26, 1216, 1861
FaeI	CATG	9 cut(s)	186, 374, 415, 815, 1169, 1195, 1562, 1586, 1727
FalI	AAGNNNNNCTT	2 cut(s)	1944, 1976
FaqI	GGGAC	5 cut(s)	47, 143, 410, 1969, 2087
FatI	CATG	9 cut(s)	182, 370, 411, 811, 1165, 1191, 1558, 1582, 1723
FauI	CCCGC	1 cut(s)	1343
FauNDI	CATATG	1 cut(s)	1852
FbaI	TGATCA	2 cut(s)	165, 1188
FblI	GTMKAC	1 cut(s)	1740
Fnu4HI	GCNGC	7 cut(s)	834, 1153, 1298, 1348, 1563, 1597, 1794
FokI	GGATG	2 cut(s)	62, 973
Fsp4HI	GCNGC	7 cut(s)	834, 1153, 1298, 1348, 1563, 1597, 1794
FspBI	CTAG	5 cut(s)	179, 837, 1310, 1928, 2039
GlaI	GCGC	5 cut(s)	7, 128, 344, 359, 992
GluI	GCNGC	7 cut(s)	834, 1153, 1298, 1348, 1563, 1597, 1794
GsuI	CTGGAG	4 cut(s)	351, 1221, 1349, 1827
HaeII	RGCGCY	1 cut(s)	130
HaeIII	GGCC	4 cut(s)	74, 1009, 1435, 1494
HgaI	GACGC	1 cut(s)	2084
HhaI	GCGC	5 cut(s)	8, 129, 345, 360, 993
Hin1I	GRCGYC	1 cut(s)	2076
Hin1II	CATG	9 cut(s)	186, 374, 415, 815, 1169, 1195, 1562, 1586, 1727
Hin6I	GCGC	5 cut(s)	6, 127, 343, 358, 991
HinP1I	GCGC	5 cut(s)	6, 127, 343, 358, 991
HincII	GTYRAC	1 cut(s)	1888
HindII	GTYRAC	1 cut(s)	1888
HinfI	GANTC	6 cut(s)	191, 308, 1631, 1640, 1673, 1982
HpaI	GTTAAC	1 cut(s)	1888
HphI	GGTGA	3 cut(s)	88, 1851, 1877
Hpy166II	GTNNAC	4 cut(s)	23, 1741, 1859, 1888
Hpy8I	GTNNAC	4 cut(s)	23, 1741, 1859, 1888
Hpy99I	CGWCG	2 cut(s)	1325, 1655
HpyAV	CCTTC	8 cut(s)	513, 663, 760, 1073, 1106, 1481, 1620, 1640
HpyCH4III	ACNGT	8 cut(s)	295, 495, 868, 1018, 1430, 1802, 1885, 2031
HpyCH4IV	ACGT	3 cut(s)	58, 754, 1746
HpyCH4V	TGCA	9 cut(s)	23, 70, 998, 1155, 1358, 1565, 1723, 1796, 2023
HpyF10VI	GCNNNNNNNGC	6 cut(s)	184, 335, 1306, 1559, 1802, 2099
HpySE526I	ACGT	3 cut(s)	58, 754, 1746
Hsp92I	GRCGYC	1 cut(s)	2076
Hsp92II	CATG	9 cut(s)	186, 374, 415, 815, 1169, 1195, 1562, 1586, 1727
HspAI	GCGC	5 cut(s)	6, 127, 343, 358, 991
Ksp22I	TGATCA	2 cut(s)	165, 1188
KspAI	GTTAAC	1 cut(s)	1888
Kzo9I	GATC	6 cut(s)	165, 510, 559, 614, 1094, 1188
LmnI	GCTCC	6 cut(s)	1121, 1202, 1528, 1579, 1604, 1808
Lsp1109I	GCAGC	6 cut(s)	1139, 1309, 1359, 1549, 1608, 1780
LweI	GCATC	3 cut(s)	325, 980, 2032
MabI	ACCWGGT	2 cut(s)	1216, 1861
MaeI	CTAG	5 cut(s)	179, 837, 1310, 1928, 2039
MaeII	ACGT	3 cut(s)	58, 754, 1746
MaeIII	GTNAC	3 cut(s)	295, 794, 1316
MalI	GATC	6 cut(s)	167, 512, 561, 616, 1096, 1190
MboI	GATC	6 cut(s)	165, 510, 559, 614, 1094, 1188
MflI	RGATCY	2 cut(s)	559, 614
MhlI	GDGCHC	3 cut(s)	25, 232, 1088
MlyI	GAGTC	2 cut(s)	200, 1991
MmeI	TCCRAC	1 cut(s)	133
MroXI	GAANNNNTTC	1 cut(s)	1872
MseI	TTAA	5 cut(s)	1260, 1622, 1712, 1887, 1989
MspA1I	CMGCKG	2 cut(s)	197, 1350
MspCI	CTTAAG	1 cut(s)	1988
MspR9I	CCNGG	3 cut(s)	28, 1218, 1863
Mva1269I	GAATGC	1 cut(s)	1513
MvaI	CCWGG	3 cut(s)	28, 1218, 1863
MvnI	CGCG	1 cut(s)	991
MwoI	GCNNNNNNNGC	6 cut(s)	184, 335, 1306, 1559, 1802, 2099
NdeI	CATATG	1 cut(s)	1852
NdeII	GATC	6 cut(s)	165, 510, 559, 614, 1094, 1188
NheI	GCTAGC	1 cut(s)	178
NlaIII	CATG	9 cut(s)	186, 374, 415, 815, 1169, 1195, 1562, 1586, 1727
NlaIV	GGNNCC	6 cut(s)	42, 616, 909, 1033, 1117, 1215
NmuCI	GTSAC	2 cut(s)	295, 1316
NspI	RCATGY	4 cut(s)	415, 1169, 1562, 1586
PaeI	GCATGC	1 cut(s)	1169
PaeR7I	CTCGAG	2 cut(s)	122, 823
PagI	TCATGA	1 cut(s)	1191
PasI	CCCWGGG	1 cut(s)	27
PciI	ACATGT	1 cut(s)	411
PctI	GAATGC	1 cut(s)	1513
PdmI	GAANNNNTTC	1 cut(s)	1872
PfeI	GAWTC	4 cut(s)	308, 1631, 1640, 1673
PflFI	GACNNNGTC	1 cut(s)	1745
PflMI	CCANNNNNTGG	1 cut(s)	1417
PkrI	GCNGC	7 cut(s)	835, 1154, 1299, 1349, 1564, 1598, 1795
PleI	GAGTC	2 cut(s)	199, 1990
PmaCI	CACGTG	1 cut(s)	59
PmlI	CACGTG	1 cut(s)	59
PpsI	GAGTC	2 cut(s)	199, 1990
Ppu21I	YACGTR	1 cut(s)	59
PpuMI	RGGWCCY	1 cut(s)	40
PscI	ACATGT	1 cut(s)	411
PsiI	TTATAA	1 cut(s)	488
Psp5II	RGGWCCY	1 cut(s)	40
Psp6I	CCWGG	3 cut(s)	26, 1216, 1861
PspCI	CACGTG	1 cut(s)	59
PspGI	CCWGG	3 cut(s)	26, 1216, 1861
PspN4I	GGNNCC	6 cut(s)	42, 616, 909, 1033, 1117, 1215
PspPI	GGNCC	3 cut(s)	40, 1007, 1434
PspPPI	RGGWCCY	1 cut(s)	40
PspXI	VCTCGAGB	1 cut(s)	823
PstI	CTGCAG	2 cut(s)	1567, 1798
PsuI	RGATCY	2 cut(s)	559, 614
PsyI	GACNNNGTC	1 cut(s)	1745
RsaI	GTAC	3 cut(s)	429, 1453, 2033
RsaNI	GTAC	3 cut(s)	428, 1452, 2032
SaqAI	TTAA	5 cut(s)	1260, 1622, 1712, 1887, 1989
SatI	GCNGC	7 cut(s)	834, 1153, 1298, 1348, 1563, 1597, 1794
Sau3AI	GATC	6 cut(s)	165, 510, 559, 614, 1094, 1188
Sau96I	GGNCC	3 cut(s)	40, 1007, 1434
SchI	GAGTC	2 cut(s)	200, 1991
ScrFI	CCNGG	3 cut(s)	28, 1218, 1863
SduI	GDGCHC	3 cut(s)	25, 232, 1088
SexAI	ACCWGGT	2 cut(s)	1216, 1861
SfaNI	GCATC	3 cut(s)	325, 980, 2032
SfcI	CTRYAG	3 cut(s)	1563, 1665, 1794
Sfr274I	CTCGAG	2 cut(s)	122, 823
SinI	GGWCC	1 cut(s)	40
SlaI	CTCGAG	2 cut(s)	122, 823
SmlI	CTYRAG	4 cut(s)	122, 188, 823, 1988
SmoI	CTYRAG	4 cut(s)	122, 188, 823, 1988
SphI	GCATGC	1 cut(s)	1169
SsiI	CCGC	3 cut(s)	195, 833, 1350
SspI	AATATT	1 cut(s)	804
SspMI	CTAG	5 cut(s)	179, 837, 1310, 1928, 2039
StyD4I	CCNGG	3 cut(s)	26, 1216, 1861
TaaI	ACNGT	8 cut(s)	295, 495, 868, 1018, 1430, 1802, 1885, 2031
TaiI	ACGT	3 cut(s)	61, 757, 1749
TaqI	TCGA	6 cut(s)	123, 204, 526, 824, 938, 967
TauI	GCSGC	1 cut(s)	836
TfiI	GAWTC	4 cut(s)	308, 1631, 1640, 1673
Tru1I	TTAA	5 cut(s)	1260, 1622, 1712, 1887, 1989
Tru9I	TTAA	5 cut(s)	1260, 1622, 1712, 1887, 1989
TscAI	CASTG	1 cut(s)	1773
TseFI	GTSAC	2 cut(s)	295, 1316
TseI	GCWGC	6 cut(s)	1152, 1297, 1347, 1562, 1596, 1793
Tsp45I	GTSAC	2 cut(s)	295, 1316
TspDTI	ATGAA	7 cut(s)	828, 1260, 1740, 1844, 2184, 2190, 2199
TspRI	CASTG	1 cut(s)	1773
Tth111I	GACNNNGTC	1 cut(s)	1745
Van91I	CCANNNNNTGG	1 cut(s)	1417
Vha464I	CTTAAG	1 cut(s)	1988
VneI	GTGCAC	1 cut(s)	21
VpaK11BI	GGWCC	1 cut(s)	40
XapI	RAATTY	8 cut(s)	104, 816, 931, 1056, 1063, 1256, 1762, 1994
XceI	RCATGY	4 cut(s)	415, 1169, 1562, 1586
XhoI	CTCGAG	2 cut(s)	122, 823
XmiI	GTMKAC	1 cut(s)	1740
XmnI	GAANNNNTTC	1 cut(s)	1872
XspI	CTAG	5 cut(s)	179, 837, 1310, 1928, 2039

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.