Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005877

Physical Location & Seq

Genomic Coordinates Forward (+)

27566665 .. 27574166

Gene Structure 7502 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1228000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGAACTGTTTATGGCAACAGATCCTAATACAAAGCAGCTTTTATATTATGAGGACAAGGCAGACGATACAAAAAGGATCTATCTATCTCGATAAGTTGTTGATCGCCGAGAATCCTACAATTACTTTACACAATGATAAACAGGATTGCCATATTGACATATGCTCTCCTGAAGTCCTCAGCCTTTTCACAGACAATTTTGATTATCAACATCTACGTCGTCACTTTTTGAAGGGATTGCTTCTTGATGATATTATGGGTTACAAAATATTCACTCATGAAATTCACTCGAGTTATGCGGCTAGAATTGACAACTTCCGAAGCTACGATACAGTTAGTAAAGACATAATTCAGAGATGGACTTACCCATTGGTGCCAGATGTGAAGTTTTTTGGGAATTCTTCAATAAAACTGGAAAGACAGGGGATGTATCGAGCATCAGACATAGGGCTATCACGCTCTGCACAGATTGGGCCTTTTACAGTCATTGGGAGTGGCACCAAGATTGGGAACAACACCAAAATTACAAATTCTGTTATTGGGGAAGGGTGCTCTATTGGATCAAATGTCTCAATAGAAGGCTCCTATATATGGGATACTGCCACCATTGAAGATGGCTGCAAGCTGAGGCATGCTATTGTATGTGATGGAGTGATAATGAAGTCTGGAGCAGTTTTGGAACCTGGTGTAGTTTTGTCTTTCAAGGTACGTTGTTATATAGGACAAAAATTTATTGTCCCTTCATACTCAAAGGTATCTCGGCTTCAGCAGCCAACTAAGCTAGATAGTGACGATGAGCTGGAGTACGCTGACAATAGCAGCGGGATTGCAGAAATGTCATGTAATCTTGGTACTGGTGGGGCTGGTTATGTTTGGTCAATTTGTGAAGGAGGCCACGATGAGGAGTGGAGGCATTCAGTTGCTCCCATTCCTCCTGACAAACTTTTGGAGGCAATACATGCTGCAGATGATGATTTGGAGCATGCTCAAGATGGCAGCTCCCTCCCACCTTCAGGAGAGTTAAAACCTGATTCTAATGATTCTGAAGGTGATGATGGAGAGTTTCGAGATGATTCTGCCTACTTTGAGAGAGAGGTTGAAGCAACCTTTCTAAGAGCTGTGCATGAAAACATACAAGTAGACCACGTCATATTAGAAGTGAATTCACTGAGGCTGTCATACAACAAGGTGGCTGCGGACTGTGCTGGAGCTTTATTTTATTCAATGATGAAATTGGCATTAGAAACTCCACATGGTTCACCAGGTGAATTGGTTCAAGCTACTGTTAACATATTTACAAATTGGAAAAAAATGCTGAAGTCTTACCTAGCAGAAATAGACGAGGAGATTGAAGTGATACTTAGATTTGAAGAAATGTGTGAGTCCTTTAAGGAATTCTCTCCTGTGTTCGACAAGATATTGCATCAACTGTACGAACTAGAAATTATACAAGAAGAAGCTATTATGAGGTGGGATGCCGAAAAGAAAAATGCAGAGGAAACAGATAGAGTTTATGTCAAGCAATCAGCGGCCTTTATTCAATGGTTGAGAGAAGCCGAAGAAGAAGATGAAGATGAAGAAGATGAAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0005085 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005851 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0019538 GO:0019899 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0051020 GO:0065007 GO:0065009 GO:0071704 GO:0097159 GO:0098772 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Hexapep Hexapep_2 NTP_transferase W2

Protein Analysis

531

Amino Acids

59.95

Weight (kDa)

4.68

Isoelectric Point (pI)

47.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LbH_EIF2B	PF25084	140 - 237	3.6e-37	EIF2B subunit epsilon LbH domain
GMPPB_C	PF25087	152 - 235	2.2e-10	GMPPB C-terminal domain
Hexapep	PF00132	161 - 194	9.5e-07	Bacterial transferase hexapeptide (six repeats)
Hexapep_GlmU	PF24894	163 - 225	1e-05	GlmU-like, C-terminal hexapeptide
W2	PF02020	448 - 526	6.9e-19	eIF4-gamma/eIF5/eIF2-epsilon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005877)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34970 AT4G18300
fragaria_vesca	FvH4_2g12670 FvH4_2g12670
malus_domestica	MD00G1051200.v1.1 MD12G1232500.v1.1 MD17G1228000.v1.1
prunus_persica	Prupe.6G335900_v2.0.a1
pyrus_communis	pycom04g19200 pycom12g21490
rosa_chinensis	RchiOBHm_Chr2g0129391 RchiOBHm_Chr6g0273391
rosa_laevigata	RLG00000013621 RLG00000019076
rosa_multiflora	Rmu_sc0001171.1_g000012 Rmu_sc0005891.1_g000011
rosa_roxburghii	Rroxscaffold_2G00114650 Rroxscaffold_7G00195420
rosa_rugosa	Rorug06G0074500
rosa_samantha	Rh2AG338500 Rh2BG346600 Rh2CG326000 Rh2DG364400 Rh6AG189600 Rh6BG193000 Rh6CG192800 Rh6DG184300
rosa_wichuraiana	Rw6G016500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	373, 497
AccI	GTMKAC	1 cut(s)	1140
AciI	CCGC	4 cut(s)	299, 822, 1196, 1529
AclWI	GGATC	3 cut(s)	16, 85, 568
AcsI	RAATTY	6 cut(s)	282, 397, 529, 728, 1162, 1394
AcuI	CTGAAG	5 cut(s)	192, 749, 996, 1065, 1337
AdeI	CACNNNGTG	1 cut(s)	1265
AfaI	GTAC	4 cut(s)	708, 806, 853, 1433
AfiI	CCNNNNNNNGG	4 cut(s)	507, 591, 901, 1013
AjiI	CACGTC	1 cut(s)	1147
AjnI	CCWGG	2 cut(s)	682, 1261
Alw21I	GWGCWC	1 cut(s)	554
Alw26I	GTCTC	1 cut(s)	574
AlwI	GGATC	3 cut(s)	16, 85, 568
Ama87I	CYCGRG	1 cut(s)	289
AoxI	GGCC	3 cut(s)	473, 892, 1530
ApeKI	GCWGC	7 cut(s)	36, 618, 769, 819, 962, 996, 1193
ApoI	RAATTY	6 cut(s)	282, 397, 529, 728, 1162, 1394
Asp700I	GAANNNNTTC	1 cut(s)	1272
AspS9I	GGNCC	1 cut(s)	473
AsuHPI	GGTGA	3 cut(s)	1061, 1251, 1277
AvaI	CYCGRG	1 cut(s)	289
BanI	GGYRCC	2 cut(s)	373, 497
BarI	GAAGNNNNNNTAC	4 cut(s)	313, 345, 747, 779
Bbv12I	GWGCWC	1 cut(s)	554
BbvCI	CCTCAGC	2 cut(s)	179, 626
BbvI	GCAGC	7 cut(s)	48, 605, 781, 831, 949, 1008, 1180
BccI	CCATC	5 cut(s)	351, 608, 641, 986, 1049
BciT130I	CCWGG	2 cut(s)	684, 1263
BciVI	GTATCC	1 cut(s)	589
BcoDI	GTCTC	1 cut(s)	574
BfaI	CTAG	4 cut(s)	303, 782, 1328, 1439
BfmI	CTRYAG	1 cut(s)	963
BfuI	GTATCC	1 cut(s)	589
BisI	GCNGC	9 cut(s)	37, 300, 619, 770, 820, 963, 997, 1194, 1530
BlsI	GCNGC	9 cut(s)	38, 301, 620, 771, 821, 964, 998, 1195, 1531
Bme1390I	CCNGG	2 cut(s)	684, 1263
BmeT110I	CYCGRG	1 cut(s)	289
BmgBI	CACGTC	1 cut(s)	1147
BmgT120I	GGNCC	1 cut(s)	473
BmiI	GGNNCC	4 cut(s)	375, 499, 583, 681
BmrFI	CCNGG	2 cut(s)	684, 1263
BmsI	GCATC	3 cut(s)	446, 1432, 1465
BpmI	CTGGAG	3 cut(s)	687, 821, 1227
Bpu10I	CCTNAGC	2 cut(s)	179, 626
BpuEI	CTTGAG	1 cut(s)	972
Bsc4I	CCNNNNNNNGG	4 cut(s)	507, 591, 901, 1013
Bse1I	ACTGG	2 cut(s)	417, 859
BseBI	CCWGG	2 cut(s)	684, 1263
BseGI	GGATG	2 cut(s)	432, 1480
BseLI	CCNNNNNNNGG	4 cut(s)	507, 591, 901, 1013
BseMII	CTCAG	3 cut(s)	193, 617, 1160
BseNI	ACTGG	2 cut(s)	417, 859
BseRI	GAGGAG	2 cut(s)	917, 1358
BseXI	GCAGC	7 cut(s)	48, 605, 781, 831, 949, 1008, 1180
BsgI	GTGCAG	1 cut(s)	447
BshFI	GGCC	3 cut(s)	475, 894, 1532
BshNI	GGYRCC	2 cut(s)	373, 497
BsiHKAI	GWGCWC	1 cut(s)	554
BsiHKCI	CYCGRG	1 cut(s)	289
BslFI	GGGAC	1 cut(s)	722
BslI	CCNNNNNNNGG	4 cut(s)	507, 591, 901, 1013
BsmAI	GTCTC	1 cut(s)	574
BsmFI	GGGAC	1 cut(s)	722
BsmI	GAATGC	1 cut(s)	913
BsnI	GGCC	3 cut(s)	475, 894, 1532
BsoBI	CYCGRG	1 cut(s)	289
Bsp1286I	GDGCHC	1 cut(s)	554
Bsp143I	GATC	4 cut(s)	21, 77, 102, 560
BspACI	CCGC	4 cut(s)	299, 822, 1196, 1529
BspANI	GGCC	3 cut(s)	475, 894, 1532
BspCNI	CTCAG	3 cut(s)	192, 618, 1161
BspHI	TCATGA	1 cut(s)	277
BspLI	GGNNCC	4 cut(s)	375, 499, 583, 681
BspMAI	CTGCAG	1 cut(s)	967
BspPI	GGATC	3 cut(s)	16, 85, 568
BspT107I	GGYRCC	2 cut(s)	373, 497
BsrI	ACTGG	2 cut(s)	417, 859
BssMI	GATC	4 cut(s)	21, 77, 102, 560
Bst2UI	CCWGG	2 cut(s)	684, 1263
Bst4CI	ACNGT	6 cut(s)	8, 334, 484, 1202, 1285, 1431
BstAPI	GCANNNNNTGC	1 cut(s)	959
BstC8I	GCNNGC	3 cut(s)	623, 633, 984
BstDEI	CTNAG	6 cut(s)	179, 626, 777, 1112, 1169, 1361
BstF5I	GGATG	2 cut(s)	432, 1480
BstKTI	GATC	4 cut(s)	24, 80, 105, 563
BstMAI	GTCTC	1 cut(s)	574
BstMBI	GATC	4 cut(s)	21, 77, 102, 560
BstMWI	GCNNNNNNNGC	4 cut(s)	769, 778, 959, 1202
BstNI	CCWGG	2 cut(s)	684, 1263
BstNSI	RCATGY	3 cut(s)	635, 962, 986
BstSCI	CCNGG	2 cut(s)	682, 1261
BstSFI	CTRYAG	1 cut(s)	963
BstV1I	GCAGC	7 cut(s)	48, 605, 781, 831, 949, 1008, 1180
BstX2I	RGATCY	2 cut(s)	21, 77
BstYI	RGATCY	2 cut(s)	21, 77
BsuI	GTATCC	1 cut(s)	589
BsuRI	GGCC	3 cut(s)	475, 894, 1532
BtrI	CACGTC	1 cut(s)	1147
BtsCI	GGATG	2 cut(s)	432, 1480
BtsIMutI	CAGTG	1 cut(s)	1166
Cac8I	GCNNGC	3 cut(s)	623, 633, 984
CciI	TCATGA	1 cut(s)	277
Cfr13I	GGNCC	1 cut(s)	473
CsiI	ACCWGGT	2 cut(s)	682, 1261
Csp6I	GTAC	4 cut(s)	707, 805, 852, 1432
CviAII	CATG	7 cut(s)	278, 632, 840, 959, 983, 1124, 1253
CviQI	GTAC	4 cut(s)	707, 805, 852, 1432
DdeI	CTNAG	6 cut(s)	179, 626, 777, 1112, 1169, 1361
DpnI	GATC	4 cut(s)	23, 79, 104, 562
DpnII	GATC	4 cut(s)	21, 77, 102, 560
DraIII	CACNNNGTG	1 cut(s)	1265
Eco57I	CTGAAG	5 cut(s)	192, 749, 996, 1065, 1337
Eco88I	CYCGRG	1 cut(s)	289
EcoRI	GAATTC	3 cut(s)	397, 1162, 1394
EcoRII	CCWGG	2 cut(s)	682, 1261
FaeI	CATG	7 cut(s)	281, 635, 843, 962, 986, 1127, 1256
FalI	AAGNNNNNCTT	2 cut(s)	1344, 1376
FaqI	GGGAC	1 cut(s)	722
FatI	CATG	7 cut(s)	277, 631, 839, 958, 982, 1123, 1252
FauI	CCCGC	1 cut(s)	815
FauNDI	CATATG	1 cut(s)	161
FblI	GTMKAC	1 cut(s)	1140
Fnu4HI	GCNGC	9 cut(s)	37, 300, 619, 770, 820, 963, 997, 1194, 1530
FokI	GGATG	2 cut(s)	439, 1487
Fsp4HI	GCNGC	9 cut(s)	37, 300, 619, 770, 820, 963, 997, 1194, 1530
FspBI	CTAG	4 cut(s)	303, 782, 1328, 1439
GluI	GCNGC	9 cut(s)	37, 300, 619, 770, 820, 963, 997, 1194, 1530
GsuI	CTGGAG	3 cut(s)	687, 821, 1227
HaeIII	GGCC	3 cut(s)	475, 894, 1532
Hin1II	CATG	7 cut(s)	281, 635, 843, 962, 986, 1127, 1256
HincII	GTYRAC	1 cut(s)	1288
HindII	GTYRAC	1 cut(s)	1288
HinfI	GANTC	5 cut(s)	112, 1031, 1040, 1073, 1382
HpaI	GTTAAC	1 cut(s)	1288
HphI	GGTGA	3 cut(s)	1061, 1251, 1277
Hpy166II	GTNNAC	3 cut(s)	1141, 1259, 1288
Hpy188I	TCNGA	4 cut(s)	320, 354, 442, 1045
Hpy188III	TCNNGA	9 cut(s)	89, 170, 245, 278, 666, 935, 989, 1014, 1067
Hpy8I	GTNNAC	3 cut(s)	1141, 1259, 1288
Hpy99I	CGWCG	1 cut(s)	222
HpyAV	CCTTC	7 cut(s)	226, 539, 572, 750, 881, 1020, 1040
HpyCH4III	ACNGT	6 cut(s)	8, 334, 484, 1202, 1285, 1431
HpyCH4IV	ACGT	3 cut(s)	217, 709, 1146
HpyCH4V	TGCA	7 cut(s)	464, 621, 830, 965, 1123, 1423, 1493
HpyF10VI	GCNNNNNNNGC	4 cut(s)	769, 778, 959, 1202
HpyF3I	CTNAG	6 cut(s)	179, 626, 777, 1112, 1169, 1361
HpySE526I	ACGT	3 cut(s)	217, 709, 1146
Hsp92II	CATG	7 cut(s)	281, 635, 843, 962, 986, 1127, 1256
KspAI	GTTAAC	1 cut(s)	1288
Kzo9I	GATC	4 cut(s)	21, 77, 102, 560
LmnI	GCTCC	6 cut(s)	587, 668, 928, 979, 1004, 1208
Lsp1109I	GCAGC	7 cut(s)	48, 605, 781, 831, 949, 1008, 1180
LweI	GCATC	3 cut(s)	446, 1432, 1465
MabI	ACCWGGT	2 cut(s)	682, 1261
MaeI	CTAG	4 cut(s)	303, 782, 1328, 1439
MaeII	ACGT	3 cut(s)	217, 709, 1146
MaeIII	GTNAC	3 cut(s)	221, 260, 788
MalI	GATC	4 cut(s)	23, 79, 104, 562
MboI	GATC	4 cut(s)	21, 77, 102, 560
MflI	RGATCY	2 cut(s)	21, 77
MhlI	GDGCHC	1 cut(s)	554
MlyI	GAGTC	1 cut(s)	1391
MroXI	GAANNNNTTC	1 cut(s)	1272
MseI	TTAA	3 cut(s)	1022, 1287, 1389
MspA1I	CMGCKG	2 cut(s)	822, 1529
MspR9I	CCNGG	2 cut(s)	684, 1263
Mva1269I	GAATGC	1 cut(s)	913
MvaI	CCWGG	2 cut(s)	684, 1263
MwoI	GCNNNNNNNGC	4 cut(s)	769, 778, 959, 1202
NdeI	CATATG	1 cut(s)	161
NdeII	GATC	4 cut(s)	21, 77, 102, 560
NlaIII	CATG	7 cut(s)	281, 635, 843, 962, 986, 1127, 1256
NlaIV	GGNNCC	4 cut(s)	375, 499, 583, 681
NmeAIII	GCCGAG	2 cut(s)	133, 739
NmuCI	GTSAC	2 cut(s)	221, 788
NspI	RCATGY	3 cut(s)	635, 962, 986
PaeI	GCATGC	2 cut(s)	635, 986
PaeR7I	CTCGAG	1 cut(s)	289
PagI	TCATGA	1 cut(s)	277
PctI	GAATGC	1 cut(s)	913
PdmI	GAANNNNTTC	1 cut(s)	1272
PfeI	GAWTC	4 cut(s)	112, 1031, 1040, 1073
PflFI	GACNNNGTC	1 cut(s)	1145
PkrI	GCNGC	9 cut(s)	38, 301, 620, 771, 821, 964, 998, 1195, 1531
PleI	GAGTC	1 cut(s)	1390
PpsI	GAGTC	1 cut(s)	1390
Psp6I	CCWGG	2 cut(s)	682, 1261
PspGI	CCWGG	2 cut(s)	682, 1261
PspN4I	GGNNCC	4 cut(s)	375, 499, 583, 681
PspPI	GGNCC	1 cut(s)	473
PspXI	VCTCGAGB	1 cut(s)	289
PstI	CTGCAG	1 cut(s)	967
PsuI	RGATCY	2 cut(s)	21, 77
PsyI	GACNNNGTC	1 cut(s)	1145
RsaI	GTAC	4 cut(s)	708, 806, 853, 1433
RsaNI	GTAC	4 cut(s)	707, 805, 852, 1432
SaqAI	TTAA	3 cut(s)	1022, 1287, 1389
SatI	GCNGC	9 cut(s)	37, 300, 619, 770, 820, 963, 997, 1194, 1530
Sau3AI	GATC	4 cut(s)	21, 77, 102, 560
Sau96I	GGNCC	1 cut(s)	473
SchI	GAGTC	1 cut(s)	1391
ScrFI	CCNGG	2 cut(s)	684, 1263
SduI	GDGCHC	1 cut(s)	554
SexAI	ACCWGGT	2 cut(s)	682, 1261
SfaNI	GCATC	3 cut(s)	446, 1432, 1465
SfcI	CTRYAG	1 cut(s)	963
Sfr274I	CTCGAG	1 cut(s)	289
SlaI	CTCGAG	1 cut(s)	289
SmlI	CTYRAG	2 cut(s)	289, 987
SmoI	CTYRAG	2 cut(s)	289, 987
SphI	GCATGC	2 cut(s)	635, 986
SsiI	CCGC	4 cut(s)	299, 822, 1196, 1529
SspI	AATATT	1 cut(s)	270
SspMI	CTAG	4 cut(s)	303, 782, 1328, 1439
StyD4I	CCNGG	2 cut(s)	682, 1261
TaaI	ACNGT	6 cut(s)	8, 334, 484, 1202, 1285, 1431
TaiI	ACGT	3 cut(s)	220, 712, 1149
TaqI	TCGA	5 cut(s)	90, 290, 433, 1066, 1410
TauI	GCSGC	2 cut(s)	302, 1532
TfiI	GAWTC	4 cut(s)	112, 1031, 1040, 1073
Tru1I	TTAA	3 cut(s)	1022, 1287, 1389
Tru9I	TTAA	3 cut(s)	1022, 1287, 1389
TscAI	CASTG	1 cut(s)	1173
TseFI	GTSAC	2 cut(s)	221, 788
TseI	GCWGC	7 cut(s)	36, 618, 769, 819, 962, 996, 1193
Tsp45I	GTSAC	2 cut(s)	221, 788
TspDTI	ATGAA	8 cut(s)	17, 294, 674, 732, 1140, 1244, 1584, 1590
TspRI	CASTG	1 cut(s)	1173
Tth111I	GACNNNGTC	1 cut(s)	1145
XapI	RAATTY	6 cut(s)	282, 397, 529, 728, 1162, 1394
XceI	RCATGY	3 cut(s)	635, 962, 986
XhoI	CTCGAG	1 cut(s)	289
XmiI	GTMKAC	1 cut(s)	1140
XmnI	GAANNNNTTC	1 cut(s)	1272
XspI	CTAG	4 cut(s)	303, 782, 1328, 1439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.