RchiOBHm_Chr6g0273391

Translation initiation factor eIF-2B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005877
Physical Location & Seq
Reverse (-)
34275927 .. 34285094
9168 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24526

Sequence Viewer

Length: 2178 bp
ATGGGTGCACAGAAGAAAGGCGCGTCTAGGGCTTCAGAAGACCCTGAGGAATTGGCACGTGTCCCACTCCAGGCCATCCTCCTCGCCGATAGTTTCACCACCAAATTCCGACCCATCACTCTTGAACGCCCCAAGGTGCTGTTGCCGTTGGTCAATGCCCCGATGATCAGTTACACGCTGGCATGGCTTGAGTCTGCTGGCGTTGAGGAGGTTTTTGTTTTCTGCTGTGCTCACTCCAAGCAAGTCATCACTTATTTGGACAATTCTGAGTGGTTTTCCCAACCAAACTTCACCGTCACCACAATTGAGTCACATGATTCTATTAGTGCCGGAGATGCTCTGCGCTTGATATATGAGCGCAATGTGATACATGGAGATTTTGTCCTAGTCAGTGGGGATACCGTGAGCAACATGTCGCTTACTCAGGTGCTTCAAGAACATAAAGAGAGAAGGAAGAAGGATAGTAATGCTGTGATGACAGTTGTTATTAAACGGTCAAAGCCTTCACCAATCACTCGCCAATCTCGACTGGGCACTGATGAGCTCTTTATGGCAGTAGATCCCAACACAAAGCAGCTCTTATATTATGAGGACAAGGCAGACCATGCAAAGGGATCTATATATCTTGATAAGTTATTGCTTGCTGATAAGTCTTCGATTTCTCTGCATAATGACAAACAGGATTGCTATATCGACATCTGCTCTCCTGAAGTCCTCAGCCTTTTCACAGACAATTTTGACTATCAACATTTACGTCGTCATTTTTTAAAGGGATTGCTTCTTGATGATATTATGGGTTACAAAATATTCACACATGAAATTCACTCGACTTATGCGGCTAGGATTGACAACTTCAGAAGCTATGATACAATCAGTAAGGACATAATTCAGAGATGGACTTACCCATTGGTGCCAGATGTTAAGTTTTTCGGAGATTCTTCAACGAAGCTGGAAAGACGGGGGATGTATCGAGCCTCAGATATAGGGCAATCACGATCTGCACAGATTGGTCCTTTTACAGTCATTGGGAATGGCACCAAGATTGGGGACAACACCAAAATTTCAAATTCTGTTATTGGAGAAGGGTGTTCTATTGGACCAAACGTTTCCATAGAAGGTTCTTATATATGGGATAATGTCACCATTGAAGATGGCTGCAAGCTAAGGCATGCTATAGTATGTGATGGAGTGATAATGAAGTCTGGGGCAGTTTTGGAATCGGGTGTAGTTTTGTCTTTCAAGGTTGTGATAGGACAACAATTTATTGTCCCTTCATACTCGAAGGTATCTTTACTTCAACAACCAATAAAGCAAGACAGTGATGAGGAGCTAGAGTATGCTGACAATAGCACTGGGATTGCAGAAATTTCCCCTCCTATACATGCACCAGATATATCGAATGGGGAAATAGCGAACCAACAACAGGAGACTCGGTGTTGGCCTACATCTGAGCTTGGTATTGGTGGGGCTGGTTATGTTTGGTCAGTTTGTGAAGGAGGCCATGATGAAGAGTGGAGGCATTCAGTTGCTCCCATTCCTGCAGAGAAACTTGCTGAGGTGATACAAGCTGCAGAGGATGATCTGGAGGTAGATCCAGATGGCAGCAGTCTCCCACCTTCAGGAGAGCTGAAACTTGATTCTGATTCTGATGAAGAGTATAGAGATGGTTCCTACTTTGAAAATGAGGTTGAAGCAACCTTCCTAAGAGCTGTGGATGAAAATATTAAAGTAGAGAATGTCGTCTTAGAAGTGAACTCACTGAGATTGTCATTCAACAAGCAAACTGTACACTGTGCTGGAGCTATACTTTATTCAATGATGAAATTGGCATTAGAAACTCCACATAATTCAGCAGTTGAATTGGTCCAAGCTACTACGAAGGTAATTACAAAATGGAAAGGACTGCTGAAGTATTACCTATCAGACATTGATGAAGAGATAGAAGTAATAATGAAATTTGAAGATATGTGTTTGGAGTCCGCTAAGGAGTTCTCCCCCGTGTTCGATAAGATACTCTATCAACTGTATGACCAAGAAGTTATACAGGAAGAGGCTATTCTGAGGTGGGATGATGAAAAGAGACATGCTGATGAATCAGATAGAATTTTTGTTAGGCAGGCTGAAACATTTATCCAATGGTTGAGAGAGGCATCTGAAGAAGAGGATGAGGAAGAGTAG

Protein Analysis

725

Amino Acids

81.57

Weight (kDa)

4.74

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 25 - 164 7.9e-12 Nucleotidyl transferase
LbH_EIF2B PF25084 319 - 422 2.6e-39 EIF2B subunit epsilon LbH domain
GMPPB_C PF25087 332 - 416 4.8e-10 GMPPB C-terminal domain
Hexapep PF00132 340 - 373 1.4e-07 Bacterial transferase hexapeptide (six repeats)
W2 PF02020 646 - 724 7.9e-18 eIF4-gamma/eIF5/eIF2-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 910, 1034
AccII CGCG 1 cut(s) 23
AciI CCGC 2 cut(s) 836, 1980
AclI AACGTT 1 cut(s) 1104
AclWI GGATC 3 cut(s) 554, 622, 1586
AcsI RAATTY 7 cut(s) 104, 819, 1059, 1066, 1365, 1955, 2103
AcuI CTGAAG 6 cut(s) 18, 729, 838, 1602, 1928, 2175
AcvI CACGTG 1 cut(s) 59
AfaI GTAC 1 cut(s) 1788
AfiI CCNNNNNNNGG 5 cut(s) 70, 610, 1044, 1423, 1619
AflIII ACRYGT 2 cut(s) 58, 411
AjnI CCWGG 1 cut(s) 69
Alw21I GWGCWC 3 cut(s) 10, 232, 546
Alw26I GTCTC 3 cut(s) 1421, 1613, 2074
Alw44I GTGCAC 1 cut(s) 6
AlwI GGATC 3 cut(s) 554, 622, 1586
AoxI GGCC 3 cut(s) 72, 1439, 1498
ApaLI GTGCAC 1 cut(s) 6
ApeKI GCWGC 4 cut(s) 574, 1155, 1568, 1602
ApoI RAATTY 7 cut(s) 104, 819, 1059, 1066, 1365, 1955, 2103
AspLEI GCGC 3 cut(s) 23, 345, 360
AspS9I GGNCC 3 cut(s) 1010, 1097, 1864
AsuHPI GGTGA 6 cut(s) 88, 283, 289, 498, 1132, 1570
AvaII GGWCC 3 cut(s) 1010, 1097, 1864
AxyI CCTNAGG 1 cut(s) 45
BaeGI GKGCMC 2 cut(s) 10, 536
BanI GGYRCC 2 cut(s) 910, 1034
BanII GRGCYC 1 cut(s) 546
BarI GAAGNNNNNNTAC 4 cut(s) 850, 882, 1278, 1310
BbrPI CACGTG 1 cut(s) 59
BbsI GAAGAC 2 cut(s) 45, 645
Bbv12I GWGCWC 3 cut(s) 10, 232, 546
BbvCI CCTCAGC 2 cut(s) 716, 1554
BbvI GCAGC 4 cut(s) 586, 1142, 1555, 1614
BccI CCATC 7 cut(s) 83, 122, 888, 1145, 1178, 1592, 1658
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 2 cut(s) 646, 680
BciT130I CCWGG 1 cut(s) 71
BciVI GTATCC 1 cut(s) 391
BclI TGATCA 1 cut(s) 165
BcoDI GTCTC 3 cut(s) 1421, 1613, 2074
BfaI CTAG 4 cut(s) 27, 386, 840, 1331
BfmI CTRYAG 3 cut(s) 1173, 1539, 1569
BfuI GTATCC 1 cut(s) 391
BisI GCNGC 5 cut(s) 575, 837, 1156, 1569, 1603
BlsI GCNGC 5 cut(s) 576, 838, 1157, 1570, 1604
Bme1390I CCNGG 1 cut(s) 71
Bme18I GGWCC 3 cut(s) 1010, 1097, 1864
BmgT120I GGNCC 3 cut(s) 1010, 1097, 1864
BmiI GGNNCC 3 cut(s) 912, 1036, 1669
BmrFI CCNGG 1 cut(s) 71
BmrI ACTGGG 2 cut(s) 539, 1362
BmsI GCATC 2 cut(s) 325, 2159
BmuI ACTGGG 2 cut(s) 539, 1362
BpiI GAAGAC 2 cut(s) 45, 645
BpmI CTGGAG 3 cut(s) 53, 1604, 1818
Bpu10I CCTNAGC 4 cut(s) 716, 1163, 1554, 1983
BpuEI CTTGAG 1 cut(s) 209
BsaAI YACGTR 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 132
BsaXI ACNNNNNCTCC 6 cut(s) 366, 396, 1319, 1349, 1418, 1448
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 610, 1044, 1423, 1619
Bse1I ACTGG 2 cut(s) 534, 1357
Bse21I CCTNAGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 367
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 6 cut(s) 75, 969, 1582, 1720, 2074, 2170
BseLI CCNNNNNNNGG 5 cut(s) 70, 610, 1044, 1423, 1619
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 9 cut(s) 36, 258, 437, 730, 990, 1440, 1545, 1751, 2051
BseNI ACTGG 2 cut(s) 534, 1357
BseRI GAGGAG 3 cut(s) 71, 221, 1340
BseSI GKGCMC 2 cut(s) 10, 536
BseXI GCAGC 4 cut(s) 586, 1142, 1555, 1614
BsgI GTGCAG 1 cut(s) 984
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 74, 1441, 1500
BshNI GGYRCC 2 cut(s) 910, 1034
BsiHKAI GWGCWC 3 cut(s) 10, 232, 546
BsiSI CCGG 1 cut(s) 330
BslFI GGGAC 3 cut(s) 47, 1061, 1253
BslI CCNNNNNNNGG 5 cut(s) 70, 610, 1044, 1423, 1619
BsmAI GTCTC 3 cut(s) 1421, 1613, 2074
BsmFI GGGAC 3 cut(s) 47, 1061, 1253
BsmI GAATGC 1 cut(s) 1519
BsnI GGCC 3 cut(s) 74, 1441, 1500
Bsp1286I GDGCHC 4 cut(s) 10, 232, 536, 546
Bsp1407I TGTACA 1 cut(s) 1786
Bsp143I GATC 6 cut(s) 165, 559, 614, 995, 1579, 1591
BspACI CCGC 2 cut(s) 836, 1980
BspANI GGCC 3 cut(s) 74, 1441, 1500
BspCNI CTCAG 9 cut(s) 37, 259, 436, 729, 989, 1441, 1546, 1752, 2052
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 3 cut(s) 912, 1036, 1669
BspMAI CTGCAG 2 cut(s) 1543, 1573
BspPI GGATC 3 cut(s) 554, 622, 1586
BspT107I GGYRCC 2 cut(s) 910, 1034
BsrDI GCAATG 1 cut(s) 367
BsrGI TGTACA 1 cut(s) 1786
BsrI ACTGG 2 cut(s) 534, 1357
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 6 cut(s) 165, 559, 614, 995, 1579, 1591
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 9 cut(s) 295, 403, 481, 495, 1021, 1319, 1786, 1793, 2025
Bst6I CTCTTC 6 cut(s) 1503, 1647, 1929, 2043, 2154, 2166
BstAPI GCANNNNNTGC 1 cut(s) 605
BstAUI TGTACA 1 cut(s) 1786
BstBAI YACGTR 1 cut(s) 59
BstC8I GCNNGC 6 cut(s) 180, 199, 642, 1160, 1170, 2118
BstF5I GGATG 6 cut(s) 75, 969, 1582, 1720, 2074, 2170
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 3 cut(s) 23, 345, 360
BstKTI GATC 6 cut(s) 168, 562, 617, 998, 1582, 1594
BstMAI GTCTC 3 cut(s) 1421, 1613, 2074
BstMBI GATC 6 cut(s) 165, 559, 614, 995, 1579, 1591
BstMWI GCNNNNNNNGC 4 cut(s) 29, 184, 335, 605
BstNI CCWGG 1 cut(s) 71
BstNSI RCATGY 4 cut(s) 415, 1172, 1385, 2087
BstSCI CCNGG 1 cut(s) 69
BstSFI CTRYAG 3 cut(s) 1173, 1539, 1569
BstSLI GKGCMC 2 cut(s) 10, 536
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 4 cut(s) 586, 1142, 1555, 1614
BstV2I GAAGAC 2 cut(s) 45, 645
BstX2I RGATCY 3 cut(s) 559, 614, 1591
BstYI RGATCY 3 cut(s) 559, 614, 1591
Bsu36I CCTNAGG 1 cut(s) 45
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 3 cut(s) 74, 1441, 1500
BtsCI GGATG 6 cut(s) 75, 969, 1582, 1720, 2074, 2170
BtsIMutI CAGTG 6 cut(s) 397, 534, 1324, 1350, 1757, 1789
Cac8I GCNNGC 6 cut(s) 180, 199, 642, 1160, 1170, 2118
CfoI GCGC 3 cut(s) 23, 345, 360
Cfr13I GGNCC 3 cut(s) 1010, 1097, 1864
CseI GACGC 1 cut(s) 12
Csp6I GTAC 1 cut(s) 1787
CviQI GTAC 1 cut(s) 1787
DpnI GATC 6 cut(s) 167, 561, 616, 997, 1581, 1593
DpnII GATC 6 cut(s) 165, 559, 614, 995, 1579, 1591
DraI TTTAAA 1 cut(s) 768
Eam1104I CTCTTC 6 cut(s) 1503, 1647, 1929, 2043, 2154, 2166
EarI CTCTTC 6 cut(s) 1503, 1647, 1929, 2043, 2154, 2166
Ecl136II GAGCTC 1 cut(s) 544
Eco130I CCWWGG 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 546
Eco47I GGWCC 3 cut(s) 1010, 1097, 1864
Eco53kI GAGCTC 1 cut(s) 544
Eco57I CTGAAG 6 cut(s) 18, 729, 838, 1602, 1928, 2175
Eco72I CACGTG 1 cut(s) 59
Eco81I CCTNAGG 1 cut(s) 45
EcoICRI GAGCTC 1 cut(s) 544
EcoRII CCWGG 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 546
ErhI CCWWGG 1 cut(s) 132
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FaqI GGGAC 3 cut(s) 47, 1061, 1253
FbaI TGATCA 1 cut(s) 165
Fnu4HI GCNGC 5 cut(s) 575, 837, 1156, 1569, 1603
FokI GGATG 5 cut(s) 62, 976, 1589, 1727, 2081
FriOI GRGCYC 1 cut(s) 546
Fsp4HI GCNGC 5 cut(s) 575, 837, 1156, 1569, 1603
FspBI CTAG 4 cut(s) 27, 386, 840, 1331
GlaI GCGC 3 cut(s) 22, 344, 359
GluI GCNGC 5 cut(s) 575, 837, 1156, 1569, 1603
GsuI CTGGAG 3 cut(s) 53, 1604, 1818
HaeIII GGCC 3 cut(s) 74, 1441, 1500
HapII CCGG 1 cut(s) 330
HgaI GACGC 1 cut(s) 12
HhaI GCGC 3 cut(s) 23, 345, 360
Hin6I GCGC 3 cut(s) 21, 343, 358
HinP1I GCGC 3 cut(s) 21, 343, 358
HpaII CCGG 1 cut(s) 330
HphI GGTGA 6 cut(s) 88, 283, 289, 498, 1132, 1570
Hpy166II GTNNAC 3 cut(s) 8, 1753, 1789
Hpy8I GTNNAC 3 cut(s) 8, 1753, 1789
Hpy99I CGWCG 1 cut(s) 759
HpyCH4III ACNGT 9 cut(s) 295, 403, 481, 495, 1021, 1319, 1786, 1793, 2025
HpyCH4IV ACGT 3 cut(s) 58, 754, 1104
HpyCH4V TGCA 9 cut(s) 8, 608, 667, 1001, 1158, 1361, 1385, 1541, 1571
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 184, 335, 605
HpySE526I ACGT 3 cut(s) 58, 754, 1104
HspAI GCGC 3 cut(s) 21, 343, 358
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 6 cut(s) 165, 559, 614, 995, 1579, 1591
LmnI GCTCC 3 cut(s) 1327, 1534, 1799
Lsp1109I GCAGC 4 cut(s) 586, 1142, 1555, 1614
LweI GCATC 2 cut(s) 325, 2159
MaeI CTAG 4 cut(s) 27, 386, 840, 1331
MaeII ACGT 3 cut(s) 58, 754, 1104
MaeIII GTNAC 5 cut(s) 170, 295, 309, 797, 1138
MalI GATC 6 cut(s) 167, 561, 616, 997, 1581, 1593
MboI GATC 6 cut(s) 165, 559, 614, 995, 1579, 1591
MfeI CAATTG 1 cut(s) 303
MflI RGATCY 3 cut(s) 559, 614, 1591
MhlI GDGCHC 4 cut(s) 10, 232, 536, 546
MlyI GAGTC 4 cut(s) 200, 317, 1423, 1985
MmeI TCCRAC 1 cut(s) 133
MseI TTAA 4 cut(s) 489, 767, 921, 1725
MslI CAYNNNNRTG 1 cut(s) 2088
MspI CCGG 1 cut(s) 330
MspR9I CCNGG 1 cut(s) 71
MunI CAATTG 1 cut(s) 303
Mva1269I GAATGC 1 cut(s) 1519
MvaI CCWGG 1 cut(s) 71
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 4 cut(s) 29, 184, 335, 605
NdeII GATC 6 cut(s) 165, 559, 614, 995, 1579, 1591
NlaIV GGNNCC 3 cut(s) 912, 1036, 1669
NmuCI GTSAC 3 cut(s) 295, 309, 1138
NspI RCATGY 4 cut(s) 415, 1172, 1385, 2087
PaeI GCATGC 1 cut(s) 1172
PciI ACATGT 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 523
PctI GAATGC 1 cut(s) 1519
PfeI GAWTC 6 cut(s) 317, 935, 1217, 1637, 1643, 2093
PkrI GCNGC 5 cut(s) 576, 838, 1157, 1570, 1604
PleI GAGTC 4 cut(s) 199, 316, 1423, 1984
PmaCI CACGTG 1 cut(s) 59
PmlI CACGTG 1 cut(s) 59
PpsI GAGTC 4 cut(s) 199, 316, 1423, 1984
Ppu21I YACGTR 1 cut(s) 59
PscI ACATGT 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 546
Psp1406I AACGTT 1 cut(s) 1104
Psp6I CCWGG 1 cut(s) 69
PspCI CACGTG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 3 cut(s) 912, 1036, 1669
PspPI GGNCC 3 cut(s) 1010, 1097, 1864
PstI CTGCAG 2 cut(s) 1543, 1573
PsuI RGATCY 3 cut(s) 559, 614, 1591
RsaI GTAC 1 cut(s) 1788
RsaNI GTAC 1 cut(s) 1787
RseI CAYNNNNRTG 1 cut(s) 2088
SacI GAGCTC 1 cut(s) 546
SaqAI TTAA 4 cut(s) 489, 767, 921, 1725
SatI GCNGC 5 cut(s) 575, 837, 1156, 1569, 1603
Sau3AI GATC 6 cut(s) 165, 559, 614, 995, 1579, 1591
Sau96I GGNCC 3 cut(s) 1010, 1097, 1864
SchI GAGTC 4 cut(s) 200, 317, 1423, 1985
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 4 cut(s) 10, 232, 536, 546
SfaNI GCATC 2 cut(s) 325, 2159
SfcI CTRYAG 3 cut(s) 1173, 1539, 1569
SinI GGWCC 3 cut(s) 1010, 1097, 1864
SmiMI CAYNNNNRTG 1 cut(s) 2088
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
SphI GCATGC 1 cut(s) 1172
SsiI CCGC 2 cut(s) 836, 1980
SspI AATATT 2 cut(s) 807, 1723
SspMI CTAG 4 cut(s) 27, 386, 840, 1331
SstI GAGCTC 1 cut(s) 546
StyD4I CCNGG 1 cut(s) 69
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 9 cut(s) 295, 403, 481, 495, 1021, 1319, 1786, 1793, 2025
TaiI ACGT 3 cut(s) 61, 757, 1107
TaqI TCGA 8 cut(s) 526, 656, 693, 827, 970, 1280, 1397, 2004
TatI WGTACW 1 cut(s) 1786
TauI GCSGC 1 cut(s) 839
TfiI GAWTC 6 cut(s) 317, 935, 1217, 1637, 1643, 2093
Tru1I TTAA 4 cut(s) 489, 767, 921, 1725
Tru9I TTAA 4 cut(s) 489, 767, 921, 1725
TscAI CASTG 6 cut(s) 397, 541, 1324, 1357, 1764, 1796
TseFI GTSAC 3 cut(s) 295, 309, 1138
TseI GCWGC 4 cut(s) 574, 1155, 1568, 1602
Tsp45I GTSAC 3 cut(s) 295, 309, 1138
TspRI CASTG 6 cut(s) 397, 541, 1324, 1357, 1764, 1796
VneI GTGCAC 1 cut(s) 6
VpaK11BI GGWCC 3 cut(s) 1010, 1097, 1864
XapI RAATTY 7 cut(s) 104, 819, 1059, 1066, 1365, 1955, 2103
XceI RCATGY 4 cut(s) 415, 1172, 1385, 2087
XspI CTAG 4 cut(s) 27, 386, 840, 1331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.