AT4G18425

endomembrane system organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013980
Physical Location & Seq
Reverse (-)
10181190 .. 10182264
1075 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18425.1

Sequence Viewer

Length: 642 bp
ATGGAGATCAAAGTTGACGAAGGTCATCAAAAAGGTACTAAAGAAGACATTACAAGGCCACTTCTTGAAGAAGACAAAGACTTCCCGGATATAGAAAGAACGACATGGATACAAAAGGCAATAGGTCAAACATTTCAAACCACTGCTCATTTAGCTAATCTCTTACCAACAGGAACCGTTCTCGCGTTTCAGCTTCTATCGCCAATTTTCTCAAATGGCGGTCAATGCGATTTAGTGAGCAAGATAATGACATCGACACTGGTAGCAATATGTGGATTCTCTTGCTTCATACTTAGCTTCACAGATTCTTACAAAGACAAAAACGGTACTATTTGCTATGGACTTGCAACAATCCATGGGTTTTGGATCATTGATGGATCCACAACTCTTCCTCAAGAGTTATCTAAGAGATATAAGCTAAGATTTATAGATTTTGTTCATGCTTTCATGTCGTTATTCGTGTTTGGTGCGGTGGTTTTATTCGATCGAAATGCGGTAAATTGTTTCTTTCCATCACCGTCAGCAGAAGCATTGGAAGTTCTTACAGCTTTGCCGGTAGGCGTAGGGGTGTTTTCTAGTATGTTGTTTGCAACATTTCCTACAACACGCAATGGGATTGGTTTTCCACTTTCTAGTAAATGA

Protein Analysis

213

Amino Acids

23.47

Weight (kDa)

5.78

Isoelectric Point (pI)

33.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 47 - 208 9.2e-70 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18425 AT5G46090
fragaria_vesca FvH4_3g08540
malus_domestica MD05G1310000.v1.1 MD10G1288800.v1.1
prunus_persica Prupe.4G052900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0009301
rosa_laevigata RLG00000031646
rosa_multiflora Rmu_sc0006233.1_g000010
rosa_roxburghii Rroxscaffold_1G00066800 Rroxscaffold_1G00067500
rosa_rugosa Rorug04G0441700
rosa_samantha Rh5AG072100 Rh5BG068600 Rh5CG081000 Rh5DG068800
rosa_wichuraiana Rw5G006740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 185
AciI CCGC 3 cut(s) 219, 470, 494
AclWI GGATC 3 cut(s) 372, 374, 385
AfaI GTAC 2 cut(s) 37, 328
AgsI TTSAA 2 cut(s) 68, 137
AluBI AGCT 5 cut(s) 155, 193, 297, 418, 548
AluI AGCT 5 cut(s) 155, 193, 297, 418, 548
AlwI GGATC 3 cut(s) 372, 374, 385
AoxI GGCC 1 cut(s) 56
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 507
BamHI GGATCC 1 cut(s) 377
BbsI GAAGAC 2 cut(s) 51, 78
BccI CCATC 2 cut(s) 368, 520
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciVI GTATCC 1 cut(s) 102
BcnI CCSGG 1 cut(s) 86
BfaI CTAG 2 cut(s) 576, 633
BfuI GTATCC 1 cut(s) 102
Bme1390I CCNGG 1 cut(s) 86
BmiI GGNNCC 2 cut(s) 175, 379
BmrFI CCNGG 1 cut(s) 86
BoxI GACNNNNGTC 1 cut(s) 21
BpiI GAAGAC 2 cut(s) 51, 78
BpuEI CTTGAG 1 cut(s) 378
BpuMI CCSGG 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 355
Bse118I RCCGGY 1 cut(s) 553
Bse1I ACTGG 1 cut(s) 264
Bse3DI GCAATG 1 cut(s) 616
BseDI CCNNGG 1 cut(s) 355
BseMI GCAATG 1 cut(s) 616
BseNI ACTGG 1 cut(s) 264
Bsh1236I CGCG 1 cut(s) 185
Bsh1285I CGRYCG 1 cut(s) 487
BshFI GGCC 1 cut(s) 58
BsiEI CGRYCG 1 cut(s) 487
BsiSI CCGG 2 cut(s) 86, 554
BsnI GGCC 1 cut(s) 58
Bsp143I GATC 4 cut(s) 6, 366, 377, 484
Bsp19I CCATGG 1 cut(s) 355
BspACI CCGC 3 cut(s) 219, 470, 494
BspANI GGCC 1 cut(s) 58
BspFNI CGCG 1 cut(s) 185
BspLI GGNNCC 2 cut(s) 175, 379
BspPI GGATC 3 cut(s) 372, 374, 385
BsrDI GCAATG 1 cut(s) 616
BsrFI RCCGGY 1 cut(s) 553
BsrI ACTGG 1 cut(s) 264
BssAI RCCGGY 1 cut(s) 553
BssECI CCNNGG 1 cut(s) 355
BssMI GATC 4 cut(s) 6, 366, 377, 484
BssT1I CCWWGG 1 cut(s) 355
Bst4CI ACNGT 3 cut(s) 178, 326, 519
Bst6I CTCTTC 1 cut(s) 393
BstDEI CTNAG 3 cut(s) 293, 405, 419
BstDSI CCRYGG 1 cut(s) 355
BstFNI CGCG 1 cut(s) 185
BstKTI GATC 4 cut(s) 9, 369, 380, 487
BstMBI GATC 4 cut(s) 6, 366, 377, 484
BstMCI CGRYCG 1 cut(s) 487
BstMWI GCNNNNNNNGC 3 cut(s) 152, 199, 225
BstPAI GACNNNNGTC 1 cut(s) 21
BstSCI CCNGG 1 cut(s) 84
BstUI CGCG 1 cut(s) 185
BstV2I GAAGAC 2 cut(s) 51, 78
BstX2I RGATCY 1 cut(s) 377
BstYI RGATCY 1 cut(s) 377
BsuI GTATCC 1 cut(s) 102
BsuRI GGCC 1 cut(s) 58
BtgI CCRYGG 1 cut(s) 355
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 2 cut(s) 141, 257
Cfr10I RCCGGY 1 cut(s) 553
Csp6I GTAC 2 cut(s) 36, 327
CviAII CATG 4 cut(s) 105, 356, 440, 448
CviJI RGCY 6 cut(s) 58, 155, 193, 297, 418, 548
CviKI_1 RGCY 6 cut(s) 58, 155, 193, 297, 418, 548
CviQI GTAC 2 cut(s) 36, 327
DdeI CTNAG 3 cut(s) 293, 405, 419
DpnI GATC 4 cut(s) 8, 368, 379, 486
DpnII GATC 4 cut(s) 6, 366, 377, 484
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 355
ErhI CCWWGG 1 cut(s) 355
FaeI CATG 4 cut(s) 108, 359, 443, 451
FatI CATG 4 cut(s) 104, 355, 439, 447
FspBI CTAG 2 cut(s) 576, 633
HaeIII GGCC 1 cut(s) 58
HapII CCGG 2 cut(s) 86, 554
Hin1II CATG 4 cut(s) 108, 359, 443, 451
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 2 cut(s) 276, 305
HpaII CCGG 2 cut(s) 86, 554
HphI GGTGA 1 cut(s) 507
Hpy166II GTNNAC 1 cut(s) 16
Hpy188III TCNNGA 2 cut(s) 65, 395
Hpy8I GTNNAC 1 cut(s) 16
HpyAV CCTTC 1 cut(s) 14
HpyCH4III ACNGT 3 cut(s) 178, 326, 519
HpyCH4V TGCA 2 cut(s) 347, 590
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 199, 225
HpyF3I CTNAG 3 cut(s) 293, 405, 419
Hsp92II CATG 4 cut(s) 108, 359, 443, 451
Kzo9I GATC 4 cut(s) 6, 366, 377, 484
LpnPI CCDG 4 cut(s) 99, 156, 245, 567
MaeI CTAG 2 cut(s) 576, 633
MalI GATC 4 cut(s) 8, 368, 379, 486
MboI GATC 4 cut(s) 6, 366, 377, 484
MboII GAAGA 4 cut(s) 56, 80, 83, 380
MflI RGATCY 1 cut(s) 377
MluCI AATT 2 cut(s) 204, 499
MnlI CCTC 1 cut(s) 402
MspI CCGG 2 cut(s) 86, 554
MspR9I CCNGG 1 cut(s) 86
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 3 cut(s) 152, 199, 225
NciI CCSGG 1 cut(s) 86
NcoI CCATGG 1 cut(s) 355
NdeII GATC 4 cut(s) 6, 366, 377, 484
NlaIII CATG 4 cut(s) 108, 359, 443, 451
NlaIV GGNNCC 2 cut(s) 175, 379
PfeI GAWTC 2 cut(s) 276, 305
PfoI TCCNGGA 1 cut(s) 84
Ple19I CGATCG 1 cut(s) 487
PshAI GACNNNNGTC 1 cut(s) 21
PspN4I GGNNCC 2 cut(s) 175, 379
PsuI RGATCY 1 cut(s) 377
PvuI CGATCG 1 cut(s) 487
RsaI GTAC 2 cut(s) 37, 328
RsaNI GTAC 2 cut(s) 36, 327
Sau3AI GATC 4 cut(s) 6, 366, 377, 484
ScrFI CCNGG 1 cut(s) 86
SetI ASST 8 cut(s) 25, 37, 127, 157, 195, 299, 420, 550
SmlI CTYRAG 1 cut(s) 393
SmoI CTYRAG 1 cut(s) 393
Sse9I AATT 2 cut(s) 204, 499
SsiI CCGC 3 cut(s) 219, 470, 494
SspMI CTAG 2 cut(s) 576, 633
StyD4I CCNGG 1 cut(s) 84
StyI CCWWGG 1 cut(s) 355
TaaI ACNGT 3 cut(s) 178, 326, 519
TaqI TCGA 3 cut(s) 254, 483, 487
TasI AATT 2 cut(s) 204, 499
TfiI GAWTC 2 cut(s) 276, 305
TscAI CASTG 2 cut(s) 148, 264
TspDTI ATGAA 3 cut(s) 277, 428, 436
TspRI CASTG 2 cut(s) 148, 264
XspI CTAG 2 cut(s) 576, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.