FvH4_3g08540

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013980
Physical Location & Seq
Forward (+)
4994531 .. 4995642
1112 bp
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UTR
Exon/CDS
Intron
FvH4_3g08540.t1

Sequence Viewer

Length: 639 bp
ATGAAGATCAGGGTAGAAGCAAAGGAATCCAACCCCGATGGTGAGCAAAAACATCCTCTCTTGCACGATACACTGTCACCGGAAATAGAGGAGAAAAGAACGCTGATACAGAGAGCCATGAACCAGACGTTTCAGAGCACAGCTCATTTAGCCAATCTTTTACCTACTGGAACAGTTCTTGCATTTCAACTTCTGGCACCTATATTCACAAACCAAGGCGTTTGCGACTCGGTCAGCAGGGTCATGACTGCTGGTCTTGTAGGCCTCTGTGGCGCTTCGTGTTTTCTACTCAGTTTCACTGATAGCTACAGGGACAACAAGGGAAACGTCTGCTATGGATTTGCTACATTCAAAGGCTTATGGATCATAGATGGGTCAACCGCCCTTGCACCAGAAGTTGCTGCAAATTTCAGACTGAAGTTTATAGATTTCCTCCATGCCTTCATGTCCATACTTGTATTTGCAGCTATTGCACTATATGATCAGAATGTAGTGAATTGCTTTGACCCAGCACCGTCGGATAAGGCTCAGGAGGTTCTCACATCATTGCCAGTTGCCATTGGTGTATTCTGCAGCATGTTGTTTGTTGTGTTTCCAACCAAGCGCCATGGGATTGGCTTCCCCCTCTCTGCAAGCTAA

Protein Analysis

213

Amino Acids

23.11

Weight (kDa)

6.18

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 45 - 207 1.2e-72 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18425 AT5G46090
fragaria_vesca FvH4_3g08540
malus_domestica MD05G1310000.v1.1 MD10G1288800.v1.1
prunus_persica Prupe.4G052900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0009301
rosa_laevigata RLG00000031646
rosa_multiflora Rmu_sc0006233.1_g000010
rosa_roxburghii Rroxscaffold_1G00066800 Rroxscaffold_1G00067500
rosa_rugosa Rorug04G0441700
rosa_samantha Rh5AG072100 Rh5BG068600 Rh5CG081000 Rh5DG068800
rosa_wichuraiana Rw5G006740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 196
AciI CCGC 1 cut(s) 381
AclWI GGATC 1 cut(s) 371
AcsI RAATTY 1 cut(s) 406
AcuI CTGAAG 1 cut(s) 437
AgsI TTSAA 2 cut(s) 188, 352
AhdI GACNNNNNGTC 1 cut(s) 252
AluBI AGCT 4 cut(s) 143, 306, 467, 636
AluI AGCT 4 cut(s) 143, 306, 467, 636
Alw21I GWGCWC 1 cut(s) 140
AlwI GGATC 1 cut(s) 371
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 3 cut(s) 401, 464, 573
ApoI RAATTY 1 cut(s) 406
AspLEI GCGC 2 cut(s) 275, 606
AsuHPI GGTGA 2 cut(s) 53, 69
BanI GGYRCC 1 cut(s) 196
Bbv12I GWGCWC 1 cut(s) 140
BbvI GCAGC 3 cut(s) 388, 476, 585
BccI CCATC 2 cut(s) 32, 365
BclI TGATCA 1 cut(s) 481
BfmI CTRYAG 2 cut(s) 307, 571
BfoI RGCGCY 2 cut(s) 276, 607
BglI GCCNNNNNGGC 1 cut(s) 270
BisI GCNGC 3 cut(s) 402, 465, 574
BlsI GCNGC 3 cut(s) 403, 466, 575
BmeRI GACNNNNNGTC 1 cut(s) 252
BmiI GGNNCC 1 cut(s) 198
BplI GAGNNNNNCTC 2 cut(s) 127, 159
Bpu10I CCTNAGC 1 cut(s) 528
BsaJI CCNNGG 2 cut(s) 214, 607
BsaWI WCCGGW 1 cut(s) 79
Bse1I ACTGG 2 cut(s) 172, 551
Bse3DI GCAATG 1 cut(s) 545
BseDI CCNNGG 2 cut(s) 214, 607
BseGI GGATG 1 cut(s) 52
BseMI GCAATG 1 cut(s) 545
BseMII CTCAG 2 cut(s) 304, 542
BseNI ACTGG 2 cut(s) 172, 551
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 3 cut(s) 388, 476, 585
BseYI CCCAGC 1 cut(s) 508
BshFI GGCC 1 cut(s) 264
BshNI GGYRCC 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 140
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 1 cut(s) 326
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 140
Bsp143I GATC 3 cut(s) 6, 363, 481
Bsp19I CCATGG 1 cut(s) 607
BspACI CCGC 1 cut(s) 381
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 2 cut(s) 303, 541
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 1 cut(s) 198
BspMAI CTGCAG 1 cut(s) 575
BspPI GGATC 1 cut(s) 371
BspT107I GGYRCC 1 cut(s) 196
BsrDI GCAATG 1 cut(s) 545
BsrI ACTGG 2 cut(s) 172, 551
BssECI CCNNGG 2 cut(s) 214, 607
BssMI GATC 3 cut(s) 6, 363, 481
BssT1I CCWWGG 2 cut(s) 214, 607
Bst4CI ACNGT 3 cut(s) 75, 175, 516
BstAPI GCANNNNNTGC 1 cut(s) 470
BstC8I GCNNGC 1 cut(s) 634
BstDEI CTNAG 2 cut(s) 290, 528
BstDSI CCRYGG 1 cut(s) 607
BstF5I GGATG 1 cut(s) 52
BstH2I RGCGCY 2 cut(s) 276, 607
BstHHI GCGC 2 cut(s) 275, 606
BstKTI GATC 3 cut(s) 9, 366, 484
BstMBI GATC 3 cut(s) 6, 363, 481
BstMWI GCNNNNNNNGC 3 cut(s) 149, 270, 470
BstNSI RCATGY 1 cut(s) 580
BstSFI CTRYAG 2 cut(s) 307, 571
BstV1I GCAGC 3 cut(s) 388, 476, 585
BstXI CCANNNNNNTGG 1 cut(s) 614
BsuRI GGCC 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 607
BtsCI GGATG 1 cut(s) 52
BtsIMutI CAGTG 2 cut(s) 71, 297
Cac8I GCNNGC 1 cut(s) 634
CciI TCATGA 1 cut(s) 243
CfoI GCGC 2 cut(s) 275, 606
CviAII CATG 6 cut(s) 118, 244, 437, 445, 577, 608
DdeI CTNAG 2 cut(s) 290, 528
DpnI GATC 3 cut(s) 8, 365, 483
DpnII GATC 3 cut(s) 6, 363, 481
DriI GACNNNNNGTC 1 cut(s) 252
Eam1105I GACNNNNNGTC 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 214, 607
Eco147I AGGCCT 1 cut(s) 264
Eco57I CTGAAG 1 cut(s) 437
EcoT14I CCWWGG 2 cut(s) 214, 607
ErhI CCWWGG 2 cut(s) 214, 607
FaeI CATG 6 cut(s) 121, 247, 440, 448, 580, 611
FaqI GGGAC 1 cut(s) 326
FatI CATG 6 cut(s) 117, 243, 436, 444, 576, 607
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 3 cut(s) 402, 465, 574
FokI GGATG 1 cut(s) 39
Fsp4HI GCNGC 3 cut(s) 402, 465, 574
GlaI GCGC 2 cut(s) 274, 605
GluI GCNGC 3 cut(s) 402, 465, 574
GsaI CCCAGC 1 cut(s) 512
HaeII RGCGCY 2 cut(s) 276, 607
HaeIII GGCC 1 cut(s) 264
HapII CCGG 1 cut(s) 80
HhaI GCGC 2 cut(s) 275, 606
Hin1II CATG 6 cut(s) 121, 247, 440, 448, 580, 611
Hin6I GCGC 2 cut(s) 273, 604
HinP1I GCGC 2 cut(s) 273, 604
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HinfI GANTC 2 cut(s) 26, 227
HpaII CCGG 1 cut(s) 80
HphI GGTGA 2 cut(s) 53, 69
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 4 cut(s) 135, 413, 486, 520
Hpy188III TCNNGA 2 cut(s) 244, 530
Hpy8I GTNNAC 1 cut(s) 378
Hpy99I CGWCG 1 cut(s) 520
HpyAV CCTTC 1 cut(s) 451
HpyCH4III ACNGT 3 cut(s) 75, 175, 516
HpyCH4IV ACGT 2 cut(s) 128, 327
HpyCH4V TGCA 8 cut(s) 64, 182, 389, 404, 464, 473, 573, 632
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 270, 470
HpyF3I CTNAG 2 cut(s) 290, 528
HpySE526I ACGT 2 cut(s) 128, 327
Hsp92II CATG 6 cut(s) 121, 247, 440, 448, 580, 611
HspAI GCGC 2 cut(s) 273, 604
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 3 cut(s) 6, 363, 481
Lsp1109I GCAGC 3 cut(s) 388, 476, 585
MaeII ACGT 2 cut(s) 128, 327
MaeIII GTNAC 1 cut(s) 75
MalI GATC 3 cut(s) 8, 365, 483
MboI GATC 3 cut(s) 6, 363, 481
MboII GAAGA 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 140
MluCI AATT 2 cut(s) 406, 496
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 3 cut(s) 54, 498, 620
MnlI CCTC 6 cut(s) 66, 82, 275, 443, 526, 635
MspI CCGG 1 cut(s) 80
MwoI GCNNNNNNNGC 3 cut(s) 149, 270, 470
NcoI CCATGG 1 cut(s) 607
NdeII GATC 3 cut(s) 6, 363, 481
NlaIII CATG 6 cut(s) 121, 247, 440, 448, 580, 611
NlaIV GGNNCC 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 75
NspI RCATGY 1 cut(s) 580
PagI TCATGA 1 cut(s) 243
PceI AGGCCT 1 cut(s) 264
PfeI GAWTC 1 cut(s) 26
PflFI GACNNNGTC 1 cut(s) 230
PkrI GCNGC 3 cut(s) 403, 466, 575
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PspFI CCCAGC 1 cut(s) 508
PspN4I GGNNCC 1 cut(s) 198
PstI CTGCAG 1 cut(s) 575
PsyI GACNNNGTC 1 cut(s) 230
SatI GCNGC 3 cut(s) 402, 465, 574
Sau3AI GATC 3 cut(s) 6, 363, 481
SchI GAGTC 1 cut(s) 221
SduI GDGCHC 1 cut(s) 140
SetI ASST 9 cut(s) 131, 145, 166, 202, 308, 330, 469, 537, 638
SfcI CTRYAG 2 cut(s) 307, 571
Sse9I AATT 2 cut(s) 406, 496
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 1 cut(s) 381
StuI AGGCCT 1 cut(s) 264
StyI CCWWGG 2 cut(s) 214, 607
TaaI ACNGT 3 cut(s) 75, 175, 516
TaiI ACGT 2 cut(s) 131, 330
TaqII GACCGA 1 cut(s) 220
TasI AATT 2 cut(s) 406, 496
TfiI GAWTC 1 cut(s) 26
TscAI CASTG 2 cut(s) 78, 304
TseFI GTSAC 1 cut(s) 75
TseI GCWGC 3 cut(s) 401, 464, 573
Tsp45I GTSAC 1 cut(s) 75
TspDTI ATGAA 3 cut(s) 17, 134, 433
TspRI CASTG 2 cut(s) 78, 304
Tth111I GACNNNGTC 1 cut(s) 230
XapI RAATTY 1 cut(s) 406
XceI RCATGY 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.