Rw5G006740

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013980
Physical Location & Seq
Reverse (-)
6977525 .. 6978214
690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G006740.1

Sequence Viewer

Length: 690 bp
ATGTTCAAAACAAACCTAGCAAAGAGACCAAAGCATACGCATTCGTTGTCAATGGAGATCAAGATAGGATCAAAGGAATCCAAGCATGATGGTGAGCAAAAACTTCCCCTCTTGCATGATACACCAGCTCCGGAAACAGATGAGAAAAGAACGTTGATACAGAGAGCCATCAACCAGACGTTTCAGAGCACAGCTCATTTGGCTAATCTTTTACCTACTGGGACAGTTCTTGCATTTCAACTTCTGGCACCTATATTTACGAACCAAGGCAACTGCGACTCGGTCAGCAGGGCCATGACTGCTGGCCTTGTAGGCCTCTGTGGCGCTTCGTGTTTTCTACTGAGTTTCACTGACAGCTATAGGGACAAGAAGGGAAATGTCTGCTATGGATTTGCTACATTCAAGGGCTTATGGGTCATCGATGGATCAGCCACCCTTGCACCAGATGTTGCAGCAAATTTCAAACTGAAGTTTATAGATTTCCTCCACGCCTTCATGTCCATACTTGTATTTGGAGCTGTTGCGTTATTTGATCAGAATGTAGTGAATTGCTTCTACCCAACGCCGTCGGATAAGGCTCAGGAGGTTCTCACATCATTGCCAGTTGCCATTGGTGTCTTCTGCAGTATGTTGTTTGTTGTGTTTCCAACCAAGCGCCATGGAATTAGCTTCCCCCTATCTGCAAGCTAA

Protein Analysis

229

Amino Acids

24.98

Weight (kDa)

8.73

Isoelectric Point (pI)

27.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 62 - 224 8.5e-73 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18425 AT5G46090
fragaria_vesca FvH4_3g08540
malus_domestica MD05G1310000.v1.1 MD10G1288800.v1.1
prunus_persica Prupe.4G052900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0009301
rosa_laevigata RLG00000031646
rosa_multiflora Rmu_sc0006233.1_g000010
rosa_roxburghii Rroxscaffold_1G00066800 Rroxscaffold_1G00067500
rosa_rugosa Rorug04G0441700
rosa_samantha Rh5AG072100 Rh5BG068600 Rh5CG081000 Rh5DG068800
rosa_wichuraiana Rw5G006740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 247
AccIII TCCGGA 1 cut(s) 130
AclI AACGTT 1 cut(s) 152
AclWI GGATC 2 cut(s) 76, 433
AcsI RAATTY 1 cut(s) 457
AcuI CTGAAG 1 cut(s) 488
AgsI TTSAA 4 cut(s) 7, 239, 403, 463
AluBI AGCT 6 cut(s) 128, 194, 357, 518, 669, 687
AluI AGCT 6 cut(s) 128, 194, 357, 518, 669, 687
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 2 cut(s) 76, 433
Aor13HI TCCGGA 1 cut(s) 130
AoxI GGCC 3 cut(s) 291, 304, 313
ApeKI GCWGC 1 cut(s) 452
ApoI RAATTY 1 cut(s) 457
Asp700I GAANNNNTTC 1 cut(s) 551
AspLEI GCGC 2 cut(s) 326, 657
AspS9I GGNCC 1 cut(s) 291
AsuHPI GGTGA 1 cut(s) 104
BanI GGYRCC 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 610
Bbv12I GWGCWC 1 cut(s) 191
BbvI GCAGC 1 cut(s) 464
BccI CCATC 3 cut(s) 83, 176, 416
BceAI ACGGC 1 cut(s) 550
BclI TGATCA 1 cut(s) 532
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 1 cut(s) 17
BfmI CTRYAG 2 cut(s) 358, 622
BfoI RGCGCY 2 cut(s) 327, 658
BglI GCCNNNNNGGC 2 cut(s) 312, 321
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 291
BmiI GGNNCC 1 cut(s) 249
BmrI ACTGGG 1 cut(s) 228
BmuI ACTGGG 1 cut(s) 228
BpiI GAAGAC 1 cut(s) 610
BplI GAGNNNNNCTC 2 cut(s) 178, 210
Bpu10I CCTNAGC 1 cut(s) 579
Bsa29I ATCGAT 1 cut(s) 420
BsaI GGTCTC 1 cut(s) 19
BsaJI CCNNGG 2 cut(s) 265, 658
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bse1I ACTGG 2 cut(s) 223, 602
Bse3DI GCAATG 1 cut(s) 596
BseAI TCCGGA 1 cut(s) 130
BseCI ATCGAT 1 cut(s) 420
BseDI CCNNGG 2 cut(s) 265, 658
BseMI GCAATG 1 cut(s) 596
BseMII CTCAG 2 cut(s) 332, 593
BseNI ACTGG 2 cut(s) 223, 602
BseXI GCAGC 1 cut(s) 464
BshFI GGCC 3 cut(s) 293, 306, 315
BshNI GGYRCC 1 cut(s) 247
BshVI ATCGAT 1 cut(s) 420
BsiHKAI GWGCWC 1 cut(s) 191
BsiSI CCGG 1 cut(s) 131
BslFI GGGAC 2 cut(s) 235, 377
BsmAI GTCTC 1 cut(s) 19
BsmFI GGGAC 2 cut(s) 235, 377
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 3 cut(s) 293, 306, 315
Bso31I GGTCTC 1 cut(s) 19
Bsp1286I GDGCHC 1 cut(s) 191
Bsp13I TCCGGA 1 cut(s) 130
Bsp143I GATC 4 cut(s) 57, 68, 425, 532
Bsp19I CCATGG 1 cut(s) 658
BspANI GGCC 3 cut(s) 293, 306, 315
BspCNI CTCAG 2 cut(s) 333, 592
BspDI ATCGAT 1 cut(s) 420
BspEI TCCGGA 1 cut(s) 130
BspLI GGNNCC 1 cut(s) 249
BspMAI CTGCAG 1 cut(s) 626
BspPI GGATC 2 cut(s) 76, 433
BspT107I GGYRCC 1 cut(s) 247
BspTNI GGTCTC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 596
BsrI ACTGG 2 cut(s) 223, 602
BssECI CCNNGG 2 cut(s) 265, 658
BssMI GATC 4 cut(s) 57, 68, 425, 532
BssT1I CCWWGG 2 cut(s) 265, 658
Bst4CI ACNGT 1 cut(s) 226
BstC8I GCNNGC 2 cut(s) 304, 685
BstDEI CTNAG 2 cut(s) 341, 579
BstDSI CCRYGG 1 cut(s) 658
BstH2I RGCGCY 2 cut(s) 327, 658
BstHHI GCGC 2 cut(s) 326, 657
BstKTI GATC 4 cut(s) 60, 71, 428, 535
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 4 cut(s) 57, 68, 425, 532
BstMWI GCNNNNNNNGC 5 cut(s) 200, 299, 312, 321, 437
BstSFI CTRYAG 2 cut(s) 358, 622
BstV1I GCAGC 1 cut(s) 464
BstV2I GAAGAC 1 cut(s) 610
Bsu15I ATCGAT 1 cut(s) 420
BsuRI GGCC 3 cut(s) 293, 306, 315
BsuTUI ATCGAT 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 658
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 2 cut(s) 304, 685
CfoI GCGC 2 cut(s) 326, 657
Cfr13I GGNCC 1 cut(s) 291
ClaI ATCGAT 1 cut(s) 420
CviAII CATG 5 cut(s) 86, 116, 295, 496, 659
DdeI CTNAG 2 cut(s) 341, 579
DpnI GATC 4 cut(s) 59, 70, 427, 534
DpnII GATC 4 cut(s) 57, 68, 425, 532
Eco130I CCWWGG 2 cut(s) 265, 658
Eco147I AGGCCT 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 488
EcoT14I CCWWGG 2 cut(s) 265, 658
ErhI CCWWGG 2 cut(s) 265, 658
FaeI CATG 5 cut(s) 89, 119, 298, 499, 662
FaqI GGGAC 2 cut(s) 235, 377
FatI CATG 5 cut(s) 85, 115, 294, 495, 658
FbaI TGATCA 1 cut(s) 532
Fnu4HI GCNGC 1 cut(s) 453
Fsp4HI GCNGC 1 cut(s) 453
FspBI CTAG 1 cut(s) 17
GlaI GCGC 2 cut(s) 325, 656
GluI GCNGC 1 cut(s) 453
HaeII RGCGCY 2 cut(s) 327, 658
HaeIII GGCC 3 cut(s) 293, 306, 315
HapII CCGG 1 cut(s) 131
HhaI GCGC 2 cut(s) 326, 657
Hin1II CATG 5 cut(s) 89, 119, 298, 499, 662
Hin6I GCGC 2 cut(s) 324, 655
HinP1I GCGC 2 cut(s) 324, 655
HinfI GANTC 2 cut(s) 77, 278
HpaII CCGG 1 cut(s) 131
HphI GGTGA 1 cut(s) 104
Hpy188I TCNGA 3 cut(s) 186, 537, 571
Hpy188III TCNNGA 3 cut(s) 61, 131, 581
Hpy99I CGWCG 1 cut(s) 571
HpyAV CCTTC 2 cut(s) 364, 502
HpyCH4III ACNGT 1 cut(s) 226
HpyCH4IV ACGT 2 cut(s) 152, 179
HpyCH4V TGCA 6 cut(s) 115, 233, 440, 452, 624, 683
HpyF10VI GCNNNNNNNGC 5 cut(s) 200, 299, 312, 321, 437
HpyF3I CTNAG 2 cut(s) 341, 579
HpySE526I ACGT 2 cut(s) 152, 179
Hsp92II CATG 5 cut(s) 89, 119, 298, 499, 662
HspAI GCGC 2 cut(s) 324, 655
Kpn2I TCCGGA 1 cut(s) 130
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 4 cut(s) 57, 68, 425, 532
LmnI GCTCC 2 cut(s) 133, 515
Lsp1109I GCAGC 1 cut(s) 464
MaeI CTAG 1 cut(s) 17
MaeII ACGT 2 cut(s) 152, 179
MalI GATC 4 cut(s) 59, 70, 427, 534
MboI GATC 4 cut(s) 57, 68, 425, 532
MboII GAAGA 1 cut(s) 610
MhlI GDGCHC 1 cut(s) 191
MluCI AATT 3 cut(s) 457, 547, 663
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 2 cut(s) 549, 671
MnlI CCTC 4 cut(s) 119, 326, 494, 577
MroI TCCGGA 1 cut(s) 130
MroXI GAANNNNTTC 1 cut(s) 551
MslI CAYNNNNRTG 1 cut(s) 90
MspI CCGG 1 cut(s) 131
Mva1269I GAATGC 1 cut(s) 40
MwoI GCNNNNNNNGC 5 cut(s) 200, 299, 312, 321, 437
NcoI CCATGG 1 cut(s) 658
NdeII GATC 4 cut(s) 57, 68, 425, 532
NlaIII CATG 5 cut(s) 89, 119, 298, 499, 662
NlaIV GGNNCC 1 cut(s) 249
PceI AGGCCT 1 cut(s) 315
PctI GAATGC 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 551
PfeI GAWTC 1 cut(s) 77
PflFI GACNNNGTC 1 cut(s) 281
PkrI GCNGC 1 cut(s) 454
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
Psp1406I AACGTT 1 cut(s) 152
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 291
PstI CTGCAG 1 cut(s) 626
PsyI GACNNNGTC 1 cut(s) 281
RseI CAYNNNNRTG 1 cut(s) 90
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 4 cut(s) 57, 68, 425, 532
Sau96I GGNCC 1 cut(s) 291
SchI GAGTC 1 cut(s) 272
SduI GDGCHC 1 cut(s) 191
SfcI CTRYAG 2 cut(s) 358, 622
SfiI GGCCNNNNNGGCC 1 cut(s) 312
SmiMI CAYNNNNRTG 1 cut(s) 90
Sse9I AATT 3 cut(s) 457, 547, 663
SseBI AGGCCT 1 cut(s) 315
SspMI CTAG 1 cut(s) 17
StuI AGGCCT 1 cut(s) 315
StyI CCWWGG 2 cut(s) 265, 658
TaaI ACNGT 1 cut(s) 226
TaiI ACGT 2 cut(s) 155, 182
TaqI TCGA 1 cut(s) 420
TaqII GACCGA 1 cut(s) 271
TasI AATT 3 cut(s) 457, 547, 663
TfiI GAWTC 1 cut(s) 77
TscAI CASTG 1 cut(s) 355
TseI GCWGC 1 cut(s) 452
TspDTI ATGAA 1 cut(s) 484
TspRI CASTG 1 cut(s) 355
Tth111I GACNNNGTC 1 cut(s) 281
XapI RAATTY 1 cut(s) 457
XmnI GAANNNNTTC 1 cut(s) 551
XspI CTAG 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.