MD05G1310000.v1.1

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013980
Physical Location & Seq
Forward (+)
44078198 .. 44078842
645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1310000.v1.1.491

Sequence Viewer

Length: 645 bp
ATGGAGATCAAGGTAGAAGCTGAGGAATCCCGGCACCTGCATGATGGTGAGCAGAAACTCCCCCTCTTGCAAGATGCACCAAAGTCCGCGACAGAAGAGGAAAGAACGCTGATACAGAAAGCCATTCGCCAGACTTTTCAGAGCACAGCTCATCTGGCCAATCTTTTGCCAACGGGAACAGTTCTTGCGTTTCAGCTTCTGGCACCCATATTTACAAACCTGGGAAACTGTGACTCAGTCAGCCGGTCCATGACTGCTGGACTTGTAGCCCTCTGTGGGGCTTCAAGTTTTCTACTGAGTTTCACTGACAGCTTTCGGGACAAGAATGGAAATGTCTGCTATGGGTTCGCTACATTCAAAGGCTTATGGATCATTGATGGATCAGCCAACATATCACCTGAAGTCGCTGCAAATTTCCGGCTGCAGTTTATAGATTTCCTCCATGCCTTCATGTCAATACTTGTATTCGCAGCTGTTGCACTTTTCGATCAGAATGTAGTGAATTGCTTCTATCCAACGCCGTCAGATGAGGCTCAGGAGGTACTCACAGCATTGCCAGTTGGCATTGGCGTCATTTGCAGTATGTTGTTTGTTGTGTTTCCAACCAAGCGCCATGGAATTGGCTTCCCGCTGTCTGCAAGTTAG

Protein Analysis

215

Amino Acids

23.28

Weight (kDa)

5.3

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 47 - 209 1e-73 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0013980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18425 AT5G46090
fragaria_vesca FvH4_3g08540
malus_domestica MD05G1310000.v1.1 MD10G1288800.v1.1
prunus_persica Prupe.4G052900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0009301
rosa_laevigata RLG00000031646
rosa_multiflora Rmu_sc0006233.1_g000010
rosa_roxburghii Rroxscaffold_1G00066800 Rroxscaffold_1G00067500
rosa_rugosa Rorug04G0441700
rosa_samantha Rh5AG072100 Rh5BG068600 Rh5CG081000 Rh5DG068800
rosa_wichuraiana Rw5G006740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 45
Acc36I ACCTGC 1 cut(s) 45
AccB1I GGYRCC 2 cut(s) 33, 202
AccII CGCG 1 cut(s) 89
AciI CCGC 2 cut(s) 87, 629
AclWI GGATC 2 cut(s) 377, 388
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 1 cut(s) 412
AcuI CTGAAG 1 cut(s) 420
AcyI GRCGYC 1 cut(s) 570
AfaI GTAC 1 cut(s) 543
AfiI CCNNNNNNNGG 2 cut(s) 276, 277
AgsI TTSAA 2 cut(s) 285, 358
AjnI CCWGG 1 cut(s) 219
AluBI AGCT 5 cut(s) 20, 149, 196, 312, 473
AluI AGCT 5 cut(s) 20, 149, 196, 312, 473
Alw21I GWGCWC 1 cut(s) 146
AlwI GGATC 2 cut(s) 377, 388
AlwNI CAGNNNCTG 1 cut(s) 199
AoxI GGCC 1 cut(s) 156
ApeKI GCWGC 3 cut(s) 407, 421, 470
ApoI RAATTY 1 cut(s) 412
Asp700I GAANNNNTTC 1 cut(s) 506
AspLEI GCGC 1 cut(s) 612
AspS9I GGNCC 1 cut(s) 246
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 2 cut(s) 59, 387
AvaII GGWCC 1 cut(s) 246
BalI TGGCCA 1 cut(s) 158
BanI GGYRCC 2 cut(s) 33, 202
Bbv12I GWGCWC 1 cut(s) 146
BbvCI CCTCAGC 1 cut(s) 21
BbvI GCAGC 3 cut(s) 394, 408, 482
BccI CCATC 2 cut(s) 38, 371
BceAI ACGGC 1 cut(s) 505
BciT130I CCWGG 1 cut(s) 221
BcnI CCSGG 1 cut(s) 31
BfmI CTRYAG 1 cut(s) 422
BfoI RGCGCY 1 cut(s) 613
BfuAI ACCTGC 1 cut(s) 45
BisI GCNGC 3 cut(s) 408, 422, 471
BlsI GCNGC 3 cut(s) 409, 423, 472
Bme1390I CCNGG 2 cut(s) 31, 221
Bme18I GGWCC 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 246
BmiI GGNNCC 2 cut(s) 35, 204
BmrFI CCNGG 2 cut(s) 31, 221
BmsI GCATC 1 cut(s) 64
BplI GAGNNNNNCTC 2 cut(s) 133, 165
Bpu10I CCTNAGC 2 cut(s) 21, 534
BpuMI CCSGG 1 cut(s) 31
BsaHI GRCGYC 1 cut(s) 570
BsaJI CCNNGG 2 cut(s) 220, 613
Bsc4I CCNNNNNNNGG 2 cut(s) 276, 277
Bse118I RCCGGY 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 557
Bse3DI GCAATG 1 cut(s) 551
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 2 cut(s) 220, 613
BseLI CCNNNNNNNGG 2 cut(s) 276, 277
BseMI GCAATG 1 cut(s) 551
BseMII CTCAG 4 cut(s) 12, 249, 287, 548
BseNI ACTGG 1 cut(s) 557
BseXI GCAGC 3 cut(s) 394, 408, 482
Bsh1236I CGCG 1 cut(s) 89
BshFI GGCC 1 cut(s) 158
BshNI GGYRCC 2 cut(s) 33, 202
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 3 cut(s) 31, 244, 418
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 276, 277
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 146
Bsp143I GATC 4 cut(s) 6, 369, 380, 487
Bsp19I CCATGG 1 cut(s) 613
BspACI CCGC 2 cut(s) 87, 629
BspANI GGCC 1 cut(s) 158
BspCNI CTCAG 4 cut(s) 13, 248, 288, 547
BspFNI CGCG 1 cut(s) 89
BspLI GGNNCC 2 cut(s) 35, 204
BspMAI CTGCAG 1 cut(s) 426
BspMI ACCTGC 1 cut(s) 45
BspPI GGATC 2 cut(s) 377, 388
BspT107I GGYRCC 2 cut(s) 33, 202
BsrDI GCAATG 1 cut(s) 551
BsrFI RCCGGY 1 cut(s) 243
BsrI ACTGG 1 cut(s) 557
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 2 cut(s) 220, 613
BssMI GATC 4 cut(s) 6, 369, 380, 487
BssNI GRCGYC 1 cut(s) 570
BssT1I CCWWGG 1 cut(s) 613
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 2 cut(s) 181, 230
Bst6I CTCTTC 1 cut(s) 90
BstACI GRCGYC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 476
BstDEI CTNAG 4 cut(s) 21, 235, 296, 534
BstDSI CCRYGG 1 cut(s) 613
BstFNI CGCG 1 cut(s) 89
BstH2I RGCGCY 1 cut(s) 613
BstHHI GCGC 1 cut(s) 612
BstKTI GATC 4 cut(s) 9, 372, 383, 490
BstMBI GATC 4 cut(s) 6, 369, 380, 487
BstMWI GCNNNNNNNGC 3 cut(s) 155, 476, 576
BstNI CCWGG 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 29, 219
BstSFI CTRYAG 1 cut(s) 422
BstUI CGCG 1 cut(s) 89
BstV1I GCAGC 3 cut(s) 394, 408, 482
BstXI CCANNNNNNTGG 1 cut(s) 620
BsuRI GGCC 1 cut(s) 158
BtgI CCRYGG 1 cut(s) 613
BtsIMutI CAGTG 1 cut(s) 303
BveI ACCTGC 1 cut(s) 45
CaiI CAGNNNCTG 1 cut(s) 199
CfoI GCGC 1 cut(s) 612
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 246
CseI GACGC 1 cut(s) 559
Csp6I GTAC 1 cut(s) 542
CviAII CATG 5 cut(s) 41, 250, 443, 451, 614
CviQI GTAC 1 cut(s) 542
DdeI CTNAG 4 cut(s) 21, 235, 296, 534
DpnI GATC 4 cut(s) 8, 371, 382, 489
DpnII GATC 4 cut(s) 6, 369, 380, 487
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 613
Eco47I GGWCC 1 cut(s) 246
Eco57I CTGAAG 1 cut(s) 420
EcoRII CCWGG 1 cut(s) 219
EcoT14I CCWWGG 1 cut(s) 613
ErhI CCWWGG 1 cut(s) 613
FaeI CATG 5 cut(s) 44, 253, 446, 454, 617
FaqI GGGAC 1 cut(s) 332
FatI CATG 5 cut(s) 40, 249, 442, 450, 613
FauI CCCGC 1 cut(s) 636
Fnu4HI GCNGC 3 cut(s) 408, 422, 471
Fsp4HI GCNGC 3 cut(s) 408, 422, 471
GlaI GCGC 1 cut(s) 611
GluI GCNGC 3 cut(s) 408, 422, 471
HaeII RGCGCY 1 cut(s) 613
HaeIII GGCC 1 cut(s) 158
HapII CCGG 3 cut(s) 31, 244, 418
HgaI GACGC 1 cut(s) 559
HhaI GCGC 1 cut(s) 612
Hin1I GRCGYC 1 cut(s) 570
Hin1II CATG 5 cut(s) 44, 253, 446, 454, 617
Hin6I GCGC 1 cut(s) 610
HinP1I GCGC 1 cut(s) 610
HinfI GANTC 2 cut(s) 26, 233
HpaII CCGG 3 cut(s) 31, 244, 418
HphI GGTGA 2 cut(s) 59, 387
Hpy188I TCNGA 3 cut(s) 141, 492, 526
Hpy188III TCNNGA 2 cut(s) 317, 536
HpyAV CCTTC 1 cut(s) 457
HpyCH4III ACNGT 2 cut(s) 181, 230
HpyCH4V TGCA 8 cut(s) 40, 70, 77, 410, 424, 479, 579, 638
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 476, 576
HpyF3I CTNAG 4 cut(s) 21, 235, 296, 534
Hsp92I GRCGYC 1 cut(s) 570
Hsp92II CATG 5 cut(s) 44, 253, 446, 454, 617
HspAI GCGC 1 cut(s) 610
Kzo9I GATC 4 cut(s) 6, 369, 380, 487
Lsp1109I GCAGC 3 cut(s) 394, 408, 482
LweI GCATC 1 cut(s) 64
MaeIII GTNAC 1 cut(s) 230
MalI GATC 4 cut(s) 8, 371, 382, 489
MboI GATC 4 cut(s) 6, 369, 380, 487
MboII GAAGA 1 cut(s) 107
MhlI GDGCHC 1 cut(s) 146
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 3 cut(s) 412, 502, 618
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 2 cut(s) 539, 626
MnlI CCTC 7 cut(s) 16, 74, 91, 281, 449, 523, 532
Mox20I TGGCCA 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 506
MscI TGGCCA 1 cut(s) 158
MslI CAYNNNNRTG 2 cut(s) 39, 45
Msp20I TGGCCA 1 cut(s) 158
MspA1I CMGCKG 2 cut(s) 473, 631
MspI CCGG 3 cut(s) 31, 244, 418
MspR9I CCNGG 2 cut(s) 31, 221
MvaI CCWGG 1 cut(s) 221
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 3 cut(s) 155, 476, 576
NciI CCSGG 1 cut(s) 31
NcoI CCATGG 1 cut(s) 613
NdeII GATC 4 cut(s) 6, 369, 380, 487
NlaIII CATG 5 cut(s) 44, 253, 446, 454, 617
NlaIV GGNNCC 2 cut(s) 35, 204
NmuCI GTSAC 1 cut(s) 230
PaqCI CACCTGC 1 cut(s) 45
PdmI GAANNNNTTC 1 cut(s) 506
PfeI GAWTC 1 cut(s) 26
PflFI GACNNNGTC 1 cut(s) 236
PkrI GCNGC 3 cut(s) 409, 423, 472
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 2 cut(s) 35, 204
PspPI GGNCC 1 cut(s) 246
PstI CTGCAG 1 cut(s) 426
PstNI CAGNNNCTG 1 cut(s) 199
PsyI GACNNNGTC 1 cut(s) 236
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 1 cut(s) 543
RsaNI GTAC 1 cut(s) 542
RseI CAYNNNNRTG 2 cut(s) 39, 45
SatI GCNGC 3 cut(s) 408, 422, 471
Sau3AI GATC 4 cut(s) 6, 369, 380, 487
Sau96I GGNCC 1 cut(s) 246
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 2 cut(s) 31, 221
SduI GDGCHC 1 cut(s) 146
SfaNI GCATC 1 cut(s) 64
SfcI CTRYAG 1 cut(s) 422
SinI GGWCC 1 cut(s) 246
SmiMI CAYNNNNRTG 2 cut(s) 39, 45
Sse9I AATT 3 cut(s) 412, 502, 618
SsiI CCGC 2 cut(s) 87, 629
StyD4I CCNGG 2 cut(s) 29, 219
StyI CCWWGG 1 cut(s) 613
TaaI ACNGT 2 cut(s) 181, 230
TaqI TCGA 1 cut(s) 486
TasI AATT 3 cut(s) 412, 502, 618
TfiI GAWTC 1 cut(s) 26
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 3 cut(s) 407, 421, 470
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 1 cut(s) 439
TspRI CASTG 1 cut(s) 310
Tth111I GACNNNGTC 1 cut(s) 236
VpaK11BI GGWCC 1 cut(s) 246
XapI RAATTY 1 cut(s) 412
XmnI GAANNNNTTC 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.