Rh5DG068800

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013980
Physical Location & Seq
Reverse (-)
5953708 .. 5967603
13896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG068800.1

Sequence Viewer

Length: 744 bp
ATGCTAACCCACGTGCAAGCCGAACTCCACGATTTGAGAAAATTCACACAATACATGTTCAAAACAAACCTAGCAAAGAGACCAAAGCATACGCATTCGTTGTCAATGGAGATCAAGATAGGATCAAAGGAATCCAAGCATGATGGTGAGCAAAAACTTCCCCTCTTGCATGATACACCAGCTCCGGAAACAGATGAGAAAAGAACGTTGATACAGAGAGCCATCAACCAGACGTTTCAGAGCACAGCTCATTTGGCTAATCTTTTACCTACTGGGACAGTTCTTGCATTTCAACTTCTGGCACCTATATTTACGAACCAAGGCAACTGCGACTCGGTCAGCAGGGCCATGACTGCTGGCCTTGTAGGCCTCTGTGGCGCTTCGTGTTTTCTACTGAGTTTCACTGACAGCTATAGGGACAAGAAGGGAAATGTCTGCTATGGATTTGCTACATTCAAAGGCTTATGGGTCATCGATGGATCAGCCACCCTTGCACCAGATGTTGCAGCAAATTTCAAACTGAAGTTTATAGATTTCCTCCACGCCTTCATGTCCATACTTGTATTTGGAGCTGTTGCGTTATTTGATCAGAATGTAGTGAATTGCTTCTACCCAACGCCGTCGGATAAGGCTCAGGAGGTTCTCACATCATTGCCAGTTGCCATTGGTGTCTTCTGCAGTATGTTGTTTGTTGTGTTTCCAACCAAGCGCCATGGAATTAGCTTCCCCCTATCTGCAAGCTAA

Protein Analysis

247

Amino Acids

27.19

Weight (kDa)

8.71

Isoelectric Point (pI)

28.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 80 - 242 1.1e-72 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18425 AT5G46090
fragaria_vesca FvH4_3g08540
malus_domestica MD05G1310000.v1.1 MD10G1288800.v1.1
prunus_persica Prupe.4G052900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0009301
rosa_laevigata RLG00000031646
rosa_multiflora Rmu_sc0006233.1_g000010
rosa_roxburghii Rroxscaffold_1G00066800 Rroxscaffold_1G00067500
rosa_rugosa Rorug04G0441700
rosa_samantha Rh5AG072100 Rh5BG068600 Rh5CG081000 Rh5DG068800
rosa_wichuraiana Rw5G006740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 301
AccIII TCCGGA 1 cut(s) 184
AclI AACGTT 1 cut(s) 206
AclWI GGATC 2 cut(s) 130, 487
AcsI RAATTY 2 cut(s) 41, 511
AcuI CTGAAG 1 cut(s) 542
AcvI CACGTG 1 cut(s) 13
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 4 cut(s) 61, 293, 457, 517
AluBI AGCT 6 cut(s) 182, 248, 411, 572, 723, 741
AluI AGCT 6 cut(s) 182, 248, 411, 572, 723, 741
Alw21I GWGCWC 1 cut(s) 245
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 2 cut(s) 130, 487
Aor13HI TCCGGA 1 cut(s) 184
AoxI GGCC 3 cut(s) 345, 358, 367
ApeKI GCWGC 1 cut(s) 506
ApoI RAATTY 2 cut(s) 41, 511
Asp700I GAANNNNTTC 1 cut(s) 605
AspLEI GCGC 2 cut(s) 380, 711
AspS9I GGNCC 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 158
BanI GGYRCC 1 cut(s) 301
BbrPI CACGTG 1 cut(s) 13
BbsI GAAGAC 1 cut(s) 664
Bbv12I GWGCWC 1 cut(s) 245
BbvI GCAGC 1 cut(s) 518
BccI CCATC 3 cut(s) 137, 230, 470
BceAI ACGGC 1 cut(s) 604
BclI TGATCA 1 cut(s) 586
BcoDI GTCTC 1 cut(s) 73
BfaI CTAG 1 cut(s) 71
BfmI CTRYAG 2 cut(s) 412, 676
BfoI RGCGCY 2 cut(s) 381, 712
BglI GCCNNNNNGGC 2 cut(s) 366, 375
BisI GCNGC 1 cut(s) 507
BlsI GCNGC 1 cut(s) 508
BmgT120I GGNCC 1 cut(s) 345
BmiI GGNNCC 1 cut(s) 303
BmrI ACTGGG 1 cut(s) 282
BmuI ACTGGG 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 664
BplI GAGNNNNNCTC 2 cut(s) 232, 264
Bpu10I CCTNAGC 1 cut(s) 633
Bsa29I ATCGAT 1 cut(s) 474
BsaAI YACGTR 1 cut(s) 13
BsaI GGTCTC 1 cut(s) 73
BsaJI CCNNGG 2 cut(s) 319, 712
BsaWI WCCGGW 1 cut(s) 184
BsaXI ACNNNNNCTCC 2 cut(s) 166, 196
Bse1I ACTGG 2 cut(s) 277, 656
Bse3DI GCAATG 1 cut(s) 650
BseAI TCCGGA 1 cut(s) 184
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 2 cut(s) 319, 712
BseMI GCAATG 1 cut(s) 650
BseMII CTCAG 2 cut(s) 386, 647
BseNI ACTGG 2 cut(s) 277, 656
BseXI GCAGC 1 cut(s) 518
BshFI GGCC 3 cut(s) 347, 360, 369
BshNI GGYRCC 1 cut(s) 301
BshVI ATCGAT 1 cut(s) 474
BsiHKAI GWGCWC 1 cut(s) 245
BsiSI CCGG 1 cut(s) 185
BslFI GGGAC 2 cut(s) 289, 431
BsmAI GTCTC 1 cut(s) 73
BsmFI GGGAC 2 cut(s) 289, 431
BsmI GAATGC 1 cut(s) 94
BsnI GGCC 3 cut(s) 347, 360, 369
Bso31I GGTCTC 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 245
Bsp13I TCCGGA 1 cut(s) 184
Bsp143I GATC 4 cut(s) 111, 122, 479, 586
Bsp19I CCATGG 1 cut(s) 712
BspANI GGCC 3 cut(s) 347, 360, 369
BspCNI CTCAG 2 cut(s) 387, 646
BspDI ATCGAT 1 cut(s) 474
BspEI TCCGGA 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 303
BspMAI CTGCAG 1 cut(s) 680
BspPI GGATC 2 cut(s) 130, 487
BspT107I GGYRCC 1 cut(s) 301
BspTNI GGTCTC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 650
BsrI ACTGG 2 cut(s) 277, 656
BssECI CCNNGG 2 cut(s) 319, 712
BssMI GATC 4 cut(s) 111, 122, 479, 586
BssT1I CCWWGG 2 cut(s) 319, 712
Bst4CI ACNGT 1 cut(s) 280
BstBAI YACGTR 1 cut(s) 13
BstC8I GCNNGC 3 cut(s) 18, 358, 739
BstDEI CTNAG 2 cut(s) 395, 633
BstDSI CCRYGG 1 cut(s) 712
BstH2I RGCGCY 2 cut(s) 381, 712
BstHHI GCGC 2 cut(s) 380, 711
BstKTI GATC 4 cut(s) 114, 125, 482, 589
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 4 cut(s) 111, 122, 479, 586
BstMWI GCNNNNNNNGC 5 cut(s) 254, 353, 366, 375, 491
BstNSI RCATGY 1 cut(s) 58
BstSFI CTRYAG 2 cut(s) 412, 676
BstV1I GCAGC 1 cut(s) 518
BstV2I GAAGAC 1 cut(s) 664
Bsu15I ATCGAT 1 cut(s) 474
BsuRI GGCC 3 cut(s) 347, 360, 369
BsuTUI ATCGAT 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 712
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 3 cut(s) 18, 358, 739
CfoI GCGC 2 cut(s) 380, 711
Cfr13I GGNCC 1 cut(s) 345
ClaI ATCGAT 1 cut(s) 474
CviAII CATG 6 cut(s) 55, 140, 170, 349, 550, 713
DdeI CTNAG 2 cut(s) 395, 633
DpnI GATC 4 cut(s) 113, 124, 481, 588
DpnII GATC 4 cut(s) 111, 122, 479, 586
Eco130I CCWWGG 2 cut(s) 319, 712
Eco147I AGGCCT 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 542
Eco72I CACGTG 1 cut(s) 13
EcoT14I CCWWGG 2 cut(s) 319, 712
ErhI CCWWGG 2 cut(s) 319, 712
FaeI CATG 6 cut(s) 58, 143, 173, 352, 553, 716
FaqI GGGAC 2 cut(s) 289, 431
FatI CATG 6 cut(s) 54, 139, 169, 348, 549, 712
FbaI TGATCA 1 cut(s) 586
Fnu4HI GCNGC 1 cut(s) 507
Fsp4HI GCNGC 1 cut(s) 507
FspBI CTAG 1 cut(s) 71
GlaI GCGC 2 cut(s) 379, 710
GluI GCNGC 1 cut(s) 507
HaeII RGCGCY 2 cut(s) 381, 712
HaeIII GGCC 3 cut(s) 347, 360, 369
HapII CCGG 1 cut(s) 185
HhaI GCGC 2 cut(s) 380, 711
Hin1II CATG 6 cut(s) 58, 143, 173, 352, 553, 716
Hin6I GCGC 2 cut(s) 378, 709
HinP1I GCGC 2 cut(s) 378, 709
HinfI GANTC 2 cut(s) 131, 332
HpaII CCGG 1 cut(s) 185
HphI GGTGA 1 cut(s) 158
Hpy188I TCNGA 3 cut(s) 240, 591, 625
Hpy188III TCNNGA 3 cut(s) 115, 185, 635
Hpy99I CGWCG 1 cut(s) 625
HpyAV CCTTC 2 cut(s) 418, 556
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 3 cut(s) 12, 206, 233
HpyCH4V TGCA 7 cut(s) 16, 169, 287, 494, 506, 678, 737
HpyF10VI GCNNNNNNNGC 5 cut(s) 254, 353, 366, 375, 491
HpyF3I CTNAG 2 cut(s) 395, 633
HpySE526I ACGT 3 cut(s) 12, 206, 233
Hsp92II CATG 6 cut(s) 58, 143, 173, 352, 553, 716
HspAI GCGC 2 cut(s) 378, 709
Kpn2I TCCGGA 1 cut(s) 184
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 4 cut(s) 111, 122, 479, 586
LmnI GCTCC 2 cut(s) 187, 569
Lsp1109I GCAGC 1 cut(s) 518
MaeI CTAG 1 cut(s) 71
MaeII ACGT 3 cut(s) 12, 206, 233
MalI GATC 4 cut(s) 113, 124, 481, 588
MboI GATC 4 cut(s) 111, 122, 479, 586
MboII GAAGA 1 cut(s) 664
MhlI GDGCHC 1 cut(s) 245
MluCI AATT 4 cut(s) 41, 511, 601, 717
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 2 cut(s) 603, 725
MnlI CCTC 4 cut(s) 173, 380, 548, 631
MroI TCCGGA 1 cut(s) 184
MroXI GAANNNNTTC 1 cut(s) 605
MslI CAYNNNNRTG 1 cut(s) 144
MspI CCGG 1 cut(s) 185
Mva1269I GAATGC 1 cut(s) 94
MwoI GCNNNNNNNGC 5 cut(s) 254, 353, 366, 375, 491
NcoI CCATGG 1 cut(s) 712
NdeII GATC 4 cut(s) 111, 122, 479, 586
NlaIII CATG 6 cut(s) 58, 143, 173, 352, 553, 716
NlaIV GGNNCC 1 cut(s) 303
NspI RCATGY 1 cut(s) 58
PceI AGGCCT 1 cut(s) 369
PciI ACATGT 1 cut(s) 54
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PctI GAATGC 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 1 cut(s) 131
PflFI GACNNNGTC 1 cut(s) 335
PkrI GCNGC 1 cut(s) 508
PleI GAGTC 1 cut(s) 326
PmaCI CACGTG 1 cut(s) 13
PmlI CACGTG 1 cut(s) 13
PpsI GAGTC 1 cut(s) 326
Ppu21I YACGTR 1 cut(s) 13
PscI ACATGT 1 cut(s) 54
Psp1406I AACGTT 1 cut(s) 206
PspCI CACGTG 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 303
PspPI GGNCC 1 cut(s) 345
PstI CTGCAG 1 cut(s) 680
PsyI GACNNNGTC 1 cut(s) 335
RseI CAYNNNNRTG 1 cut(s) 144
SatI GCNGC 1 cut(s) 507
Sau3AI GATC 4 cut(s) 111, 122, 479, 586
Sau96I GGNCC 1 cut(s) 345
SchI GAGTC 1 cut(s) 326
SduI GDGCHC 1 cut(s) 245
SfcI CTRYAG 2 cut(s) 412, 676
SfiI GGCCNNNNNGGCC 1 cut(s) 366
SmiMI CAYNNNNRTG 1 cut(s) 144
Sse9I AATT 4 cut(s) 41, 511, 601, 717
SseBI AGGCCT 1 cut(s) 369
SspMI CTAG 1 cut(s) 71
StuI AGGCCT 1 cut(s) 369
StyI CCWWGG 2 cut(s) 319, 712
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 3 cut(s) 15, 209, 236
TaqI TCGA 1 cut(s) 474
TaqII GACCGA 1 cut(s) 325
TasI AATT 4 cut(s) 41, 511, 601, 717
TfiI GAWTC 1 cut(s) 131
TscAI CASTG 1 cut(s) 409
TseI GCWGC 1 cut(s) 506
TspDTI ATGAA 1 cut(s) 538
TspRI CASTG 1 cut(s) 409
Tth111I GACNNNGTC 1 cut(s) 335
XapI RAATTY 2 cut(s) 41, 511
XceI RCATGY 1 cut(s) 58
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.