Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005801

Physical Location & Seq

Genomic Coordinates Forward (+)

10196954 .. 10201787

Gene Structure 4834 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G18465.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2088 bp

ATGGCGTTTTGGAAACCAGGGACCGAGAAACCTCGTTTCGAAGAGGATGGAGAAGGAGGCATAGTGTTCATGTCCAACAATCTCGCATCCTCTTCTTCTTCTAGCTATGGGTATGCGAATATAGAGAAGCAAAGACAGAGATTGCCAGTGTACAAGTACAGAACTGAGATTCTGTATTTGGTGGAGAATCACGCTACCACCATTATCGTTGGCGAGACGGGTAGTGGTAAAACTACTCAGATTCCTCAGTACCTGAAAGAAGCTGGGTGGGCTGAAGGAGGCCGAGTTATTGCTTGCACACAGCCAAGACGTTTAGCTGTCCAGGCGGTTTCTGCGAGAGTGGCCGAGGAAATGGGAGTGAATCTTGGGGAGGAAGTTGGCTACACTATTCGATTTGAAGATCATACAACTTCCGGTGTGACTAGTGTAAAATTTCTCACAGATGGGGTACTAATTCGAGAGATGATGGAGGATCCCCTCTTGACAAAGTATAGTGTTATTATGATAGATGAAGCTCATGAGAGATCTATTTCAACTGACATTTTACTCGGTCTCTTAAAAAAGATACAACGTCGTCGTCCTGAGCTACGCCTGATTATATCTTCAGCCACAATCGAAGCAAAAACCATGTCCAATTTCTTCAATTCCAGCAAAAAACGCCATGCGCCAGAAGGTAGTACCCCTGGACCAAAGTTGGAACCTGCAATCTTATCTGTTGAGGGCAGAGGCTTCAGTGTGAAAATTCACTATGTCGAGGAGCCTGTATCAGATTATATCCGATCAGTTGTTTCAACGATACTGTTGATTAATGAACGGGAGCCACCTGGGGATGTTCTTGTATTTCTTACTGGTCAAGAGGATATTGAAACTGCTATTAAACTTCTTGAAGAAGAAGCTCATAGCAATCAAAAGAATTCTTCAGGACTGCTACCTCTTCCATTATATTCAGGACTTTCTCGATCAGAACAGGAATTGATCTTTACTCCAACTCCCAGAGGAAAGAGAAAAGTTATACTATCAACAAATATTGCAGAAACTTCATTGACTCTAGAGGGCGTTGTCTATGTGATTGATAGTGGTTTCTCGAAGCAGAAGTTCTATAACCCAATTTCAGATATCGAAAGTCTTGTGGTGGCTCCAATATCAAAAGCATCTGCAAGGCAAAGGTCTGGTAGGGCTGGGCGAGTTCGGCCTGGGAAGTGTTACAGGCTTTACACCGAAGATTATTTTCTCAACCAAATGCCTGGAGAAGGCATACCGGAGATGCAGAGGTCGAATCTTGTTTCTACTGTAATACAGCTAAAAGCTTTGGGCATAGATAATATCTTGGGTTTCGATTGGCCTGCACCTCCATCGTCTGAAGCAATGATTCGAGCACTTGAAGTACTTTATTCACTTCAGATCCTCGATGATGATGCTAAACTCACTTCGCCAACAGGATTCCAAGTTGCAGAACTTCCACTGGACCCAATGATATCAAAAATGATTTTGGCTTCAAGTGAGCTTGGTTGTTCACATGAGATCATTACAATCGCTGCAGTTCTCTCTGTCCAATCTGTTTGGATCATTGCCCGGGGAGTACAGAAAGAACAAGATGAAGCAAAACTGAGATTTGCAGCCGCAGAGGGTGACCATGTCACATTCCTGAATGTATACAAAGGGTTTCTTGAGTCGAAAAAGCCTACACAGTGGTGTTACAAGAATTTCCTCAACTACCAATCCATGAAAAAAGTAGTTGAAATCAGAGATCAACTCAAACGGATTGCTCGGAGATTAGGCATCACCTTGAAATCATGTGACGGAGACATGGAGGCTGTGAGAAAAGCTGTGACTGCAGGTTTTTTTGCCAATGCATGCCGTTTAGAACCACACAGCAATGGAGTATACAAGACCATTAGAGGCTCTGAAGAAGTTTATATTCATCCGTCATCTGTATTATTCAGGGTGAATCCAAAATGGGTGGTTTACCAATCCATTGTCTCAACTGAGCGTCAATACATGCGGAATGTCGTCACCATCAATCCTTCTTGGCTGACAGAAGTTGCTCCACACTTTTACCAGAACCGGCAAAACGCTATGTCCTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030054 GO:0032991 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0055044 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_22 HA2 Helicase_C OB_NTP_bind

Protein Analysis

695

Amino Acids

77.71

Weight (kDa)

8.01

Isoelectric Point (pI)

50.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	60 - 210	3.5e-06	DEAD/DEAH box helicase
AAA_22	PF13401	66 - 191	2.8e-06	AAA domain
Helicase_C	PF00271	267 - 396	3.8e-13	Helicase conserved C-terminal domain
HA2_C	PF21010	489 - 548	9e-13	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	606 - 684	2e-23	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005801)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18465
fragaria_vesca	FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450
malus_domestica	MD05G1305600.v1.1
prunus_persica	Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1
pyrus_communis	pycom05g28290
rosa_chinensis	RchiOBHm_Chr2g0158101 RchiOBHm_Chr5g0009991
rosa_laevigata	RLG00000031717
rosa_multiflora	Rmu_sc0001648.1_g000001 Rmu_sc0002275.1_g000005
rosa_roxburghii	Rroxscaffold_1G00066170
rosa_rugosa	Rorug04G0448300 Rorug04G0448400 Rorug04G0448500
rosa_samantha	Rh5AG079200 Rh5BG074000 Rh5CG086600 Rh5DG074700
rosa_wichuraiana	Rw5G007330 Rw7G028410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	709, 1826
AccI	GTMKAC	2 cut(s)	1653, 1884
AciI	CCGC	3 cut(s)	326, 1620, 2002
AclWI	GGATC	4 cut(s)	467, 480, 1396, 1571
AcoI	YGGCCR	1 cut(s)	342
AcsI	RAATTY	4 cut(s)	431, 741, 913, 1702
AcuI	CTGAAG	7 cut(s)	294, 588, 715, 903, 1380, 1382, 1926
AfaI	GTAC	7 cut(s)	152, 158, 251, 450, 679, 1386, 1581
AfiI	CCNNNNNNNGG	1 cut(s)	1250
AhlI	ACTAGT	1 cut(s)	422
AjnI	CCWGG	6 cut(s)	16, 321, 682, 823, 1192, 1243
AleI	CACNNNNGTG	1 cut(s)	1690
Alw21I	GWGCWC	1 cut(s)	1378
Alw26I	GTCTC	4 cut(s)	209, 557, 1797, 1984
AlwI	GGATC	4 cut(s)	467, 480, 1396, 1571
AlwNI	CAGNNNCTG	1 cut(s)	253
Ama87I	CYCGRG	1 cut(s)	1572
AoxI	GGCC	4 cut(s)	280, 342, 1190, 1340
ApeKI	GCWGC	2 cut(s)	1535, 1616
ApoI	RAATTY	4 cut(s)	431, 741, 913, 1702
ArsI	GACNNNNNNTTYG	2 cut(s)	614, 646
AseI	ATTAAT	1 cut(s)	807
AspLEI	GCGC	1 cut(s)	667
AspS9I	GGNCC	3 cut(s)	21, 686, 1465
AsuC2I	CCSGG	2 cut(s)	1573, 1574
AsuHPI	GGTGA	4 cut(s)	1640, 1774, 1957, 2005
AsuII	TTCGAA	1 cut(s)	39
AvaI	CYCGRG	1 cut(s)	1572
AvaII	GGWCC	3 cut(s)	21, 686, 1465
BamHI	GGATCC	1 cut(s)	472
Bbv12I	GWGCWC	1 cut(s)	1378
BbvI	GCAGC	2 cut(s)	1522, 1628
BccI	CCATC	5 cut(s)	41, 437, 460, 1360, 2024
BceAI	ACGGC	1 cut(s)	1842
BcgI	CGANNNNNNTGC	6 cut(s)	1325, 1335, 1359, 1369, 1397, 1431
BciT130I	CCWGG	6 cut(s)	18, 323, 684, 825, 1194, 1245
BcnI	CCSGG	2 cut(s)	1573, 1574
BcoDI	GTCTC	4 cut(s)	209, 557, 1797, 1984
BcuI	ACTAGT	1 cut(s)	422
BfaI	CTAG	3 cut(s)	102, 423, 1049
BfmI	CTRYAG	2 cut(s)	1536, 1833
BfuAI	ACCTGC	2 cut(s)	709, 1826
BglII	AGATCT	1 cut(s)	524
BisI	GCNGC	3 cut(s)	1536, 1617, 1620
BlsI	GCNGC	3 cut(s)	1537, 1618, 1621
BmcAI	AGTACT	1 cut(s)	1386
Bme1390I	CCNGG	8 cut(s)	18, 323, 684, 825, 1194, 1245, 1573, 1574
Bme18I	GGWCC	3 cut(s)	21, 686, 1465
BmeT110I	CYCGRG	1 cut(s)	1572
BmgT120I	GGNCC	3 cut(s)	21, 686, 1465
BmiI	GGNNCC	7 cut(s)	22, 474, 699, 759, 819, 1137, 1467
BmrFI	CCNGG	8 cut(s)	18, 323, 684, 825, 1194, 1245, 1573, 1574
BmsI	GCATC	5 cut(s)	95, 1160, 1254, 1405, 1788
BplI	GAGNNNNNCTC	2 cut(s)	1737, 1769
BpmI	CTGGAG	1 cut(s)	1266
Bpu10I	CCTNAGC	1 cut(s)	582
Bpu14I	TTCGAA	1 cut(s)	39
BpuEI	CTTGAG	1 cut(s)	1688
BpuMI	CCSGG	2 cut(s)	1573, 1574
BsaI	GGTCTC	1 cut(s)	557
BsaJI	CCNNGG	7 cut(s)	17, 345, 682, 824, 1193, 1572, 1573
BsaWI	WCCGGW	2 cut(s)	413, 1258
BsaXI	ACNNNNNCTCC	6 cut(s)	48, 78, 270, 300, 973, 1003
Bsc4I	CCNNNNNNNGG	1 cut(s)	1250
Bse118I	RCCGGY	1 cut(s)	2064
Bse1I	ACTGG	3 cut(s)	146, 853, 1467
Bse3DI	GCAATG	3 cut(s)	1371, 1566, 1882
BseBI	CCWGG	6 cut(s)	18, 323, 684, 825, 1194, 1245
BseDI	CCNNGG	7 cut(s)	17, 345, 682, 824, 1193, 1572, 1573
BseGI	GGATG	4 cut(s)	52, 86, 835, 1921
BseLI	CCNNNNNNNGG	1 cut(s)	1250
BseMI	GCAATG	3 cut(s)	1371, 1566, 1882
BseMII	CTCAG	6 cut(s)	156, 251, 260, 573, 1598, 1977
BseNI	ACTGG	3 cut(s)	146, 853, 1467
BseRI	GAGGAG	1 cut(s)	770
BseXI	GCAGC	2 cut(s)	1522, 1628
BseYI	CCCAGC	2 cut(s)	263, 1178
BsgI	GTGCAG	1 cut(s)	1329
BshFI	GGCC	4 cut(s)	282, 344, 1192, 1342
BsiHKAI	GWGCWC	1 cut(s)	1378
BsiHKCI	CYCGRG	1 cut(s)	1572
BsiSI	CCGG	4 cut(s)	414, 1259, 1573, 2065
BslFI	GGGAC	1 cut(s)	34
BslI	CCNNNNNNNGG	1 cut(s)	1250
BsmAI	GTCTC	4 cut(s)	209, 557, 1797, 1984
BsmBI	CGTCTC	1 cut(s)	209
BsmFI	GGGAC	1 cut(s)	34
BsnI	GGCC	4 cut(s)	282, 344, 1192, 1342
Bso31I	GGTCTC	1 cut(s)	557
BsoBI	CYCGRG	1 cut(s)	1572
Bsp119I	TTCGAA	1 cut(s)	39
Bsp1286I	GDGCHC	1 cut(s)	1378
Bsp1407I	TGTACA	1 cut(s)	150
BspACI	CCGC	3 cut(s)	326, 1620, 2002
BspANI	GGCC	4 cut(s)	282, 344, 1192, 1342
BspCNI	CTCAG	6 cut(s)	157, 250, 259, 574, 1599, 1978
BspHI	TCATGA	1 cut(s)	517
BspLI	GGNNCC	7 cut(s)	22, 474, 699, 759, 819, 1137, 1467
BspMAI	CTGCAG	2 cut(s)	1540, 1837
BspMI	ACCTGC	2 cut(s)	709, 1826
BspPI	GGATC	4 cut(s)	467, 480, 1396, 1571
BspT104I	TTCGAA	1 cut(s)	39
BspTNI	GGTCTC	1 cut(s)	557
BsrDI	GCAATG	3 cut(s)	1371, 1566, 1882
BsrFI	RCCGGY	1 cut(s)	2064
BsrGI	TGTACA	1 cut(s)	150
BsrI	ACTGG	3 cut(s)	146, 853, 1467
BssAI	RCCGGY	1 cut(s)	2064
BssECI	CCNNGG	7 cut(s)	17, 345, 682, 824, 1193, 1572, 1573
BssNAI	GTATAC	2 cut(s)	1654, 1885
Bst1107I	GTATAC	2 cut(s)	1654, 1885
Bst2UI	CCWGG	6 cut(s)	18, 323, 684, 825, 1194, 1245
Bst4CI	ACNGT	3 cut(s)	801, 1291, 1689
Bst6I	CTCTTC	3 cut(s)	36, 97, 939
BstAUI	TGTACA	1 cut(s)	150
BstBI	TTCGAA	1 cut(s)	39
BstC8I	GCNNGC	3 cut(s)	295, 1344, 1855
BstDEI	CTNAG	6 cut(s)	165, 237, 246, 582, 1607, 1986
BstEII	GGTNACC	1 cut(s)	1628
BstENI	CCTNNNNNAGG	1 cut(s)	1248
BstF5I	GGATG	4 cut(s)	52, 86, 835, 1921
BstHHI	GCGC	1 cut(s)	667
BstMAI	GTCTC	4 cut(s)	209, 557, 1797, 1984
BstMWI	GCNNNNNNNGC	7 cut(s)	269, 323, 332, 341, 657, 1189, 1832
BstNI	CCWGG	6 cut(s)	18, 323, 684, 825, 1194, 1245
BstNSI	RCATGY	2 cut(s)	1857, 2002
BstPI	GGTNACC	1 cut(s)	1628
BstSCI	CCNGG	8 cut(s)	16, 321, 682, 823, 1192, 1243, 1571, 1572
BstSFI	CTRYAG	2 cut(s)	1536, 1833
BstV1I	GCAGC	2 cut(s)	1522, 1628
BstX2I	RGATCY	3 cut(s)	472, 524, 1401
BstXI	CCANNNNNNTGG	1 cut(s)	1244
BstYI	RGATCY	3 cut(s)	472, 524, 1401
BstZ17I	GTATAC	2 cut(s)	1654, 1885
BsuRI	GGCC	4 cut(s)	282, 344, 1192, 1342
BtsCI	GGATG	4 cut(s)	52, 86, 835, 1921
BtsIMutI	CAGTG	4 cut(s)	153, 739, 1460, 1694
BveI	ACCTGC	2 cut(s)	709, 1826
Cac8I	GCNNGC	3 cut(s)	295, 1344, 1855
CaiI	CAGNNNCTG	1 cut(s)	253
CciI	TCATGA	1 cut(s)	517
CfoI	GCGC	1 cut(s)	667
Cfr10I	RCCGGY	1 cut(s)	2064
Cfr13I	GGNCC	3 cut(s)	21, 686, 1465
Cfr9I	CCCGGG	1 cut(s)	1572
CseI	GACGC	1 cut(s)	1979
Csp6I	GTAC	7 cut(s)	151, 157, 250, 449, 678, 1385, 1580
CspCI	CAANNNNNGTGG	2 cut(s)	1941, 1976
CviQI	GTAC	7 cut(s)	151, 157, 250, 449, 678, 1385, 1580
DdeI	CTNAG	6 cut(s)	165, 237, 246, 582, 1607, 1986
EaeI	YGGCCR	1 cut(s)	342
Eam1104I	CTCTTC	3 cut(s)	36, 97, 939
EarI	CTCTTC	3 cut(s)	36, 97, 939
Eco31I	GGTCTC	1 cut(s)	557
Eco32I	GATATC	2 cut(s)	1117, 1476
Eco47I	GGWCC	3 cut(s)	21, 686, 1465
Eco57I	CTGAAG	7 cut(s)	294, 588, 715, 903, 1380, 1382, 1926
Eco88I	CYCGRG	1 cut(s)	1572
Eco91I	GGTNACC	1 cut(s)	1628
EcoNI	CCTNNNNNAGG	1 cut(s)	1248
EcoO65I	GGTNACC	1 cut(s)	1628
EcoRI	GAATTC	1 cut(s)	913
EcoRII	CCWGG	6 cut(s)	16, 321, 682, 823, 1192, 1243
EcoRV	GATATC	2 cut(s)	1117, 1476
EcoT22I	ATGCAT	1 cut(s)	1855
Esp3I	CGTCTC	1 cut(s)	209
FalI	AAGNNNNNCTT	2 cut(s)	1650, 1682
FaqI	GGGAC	1 cut(s)	34
FblI	GTMKAC	2 cut(s)	1653, 1884
Fnu4HI	GCNGC	3 cut(s)	1536, 1617, 1620
FokI	GGATG	4 cut(s)	59, 73, 842, 1908
Fsp4HI	GCNGC	3 cut(s)	1536, 1617, 1620
FspBI	CTAG	3 cut(s)	102, 423, 1049
GlaI	GCGC	1 cut(s)	666
GluI	GCNGC	3 cut(s)	1536, 1617, 1620
GsaI	CCCAGC	2 cut(s)	267, 1182
GsuI	CTGGAG	1 cut(s)	1266
HaeIII	GGCC	4 cut(s)	282, 344, 1192, 1342
HapII	CCGG	4 cut(s)	414, 1259, 1573, 2065
HgaI	GACGC	1 cut(s)	1979
HhaI	GCGC	1 cut(s)	667
Hin6I	GCGC	1 cut(s)	665
HinP1I	GCGC	1 cut(s)	665
HindIII	AAGCTT	1 cut(s)	1305
HpaII	CCGG	4 cut(s)	414, 1259, 1573, 2065
HphI	GGTGA	4 cut(s)	1640, 1774, 1957, 2005
Hpy166II	GTNNAC	5 cut(s)	151, 1514, 1654, 1885, 1966
Hpy8I	GTNNAC	5 cut(s)	151, 1514, 1654, 1885, 1966
Hpy99I	CGWCG	2 cut(s)	576, 579
HpyAV	CCTTC	5 cut(s)	47, 269, 665, 1244, 2034
HpyCH4III	ACNGT	3 cut(s)	801, 1291, 1689
HpyCH4IV	ACGT	2 cut(s)	310, 571
HpyF10VI	GCNNNNNNNGC	7 cut(s)	269, 323, 332, 341, 657, 1189, 1832
HpyF3I	CTNAG	6 cut(s)	165, 237, 246, 582, 1607, 1986
HpySE526I	ACGT	2 cut(s)	310, 571
HspAI	GCGC	1 cut(s)	665
LmnI	GCTCC	4 cut(s)	757, 817, 1141, 2050
Lsp1109I	GCAGC	2 cut(s)	1522, 1628
LweI	GCATC	5 cut(s)	95, 1160, 1254, 1405, 1788
MaeI	CTAG	3 cut(s)	102, 423, 1049
MaeII	ACGT	2 cut(s)	310, 571
MaeIII	GTNAC	8 cut(s)	418, 1202, 1628, 1636, 1694, 1796, 1828, 2011
MflI	RGATCY	3 cut(s)	472, 524, 1401
MhlI	GDGCHC	1 cut(s)	1378
MluCI	AATT	9 cut(s)	431, 453, 634, 643, 741, 913, 971, 1107, 1702
MlyI	GAGTC	2 cut(s)	1039, 1679
MmeI	TCCRAC	3 cut(s)	99, 675, 1010
Mph1103I	ATGCAT	1 cut(s)	1855
MseI	TTAA	3 cut(s)	557, 807, 876
MslI	CAYNNNNRTG	2 cut(s)	1690, 1875
MspI	CCGG	4 cut(s)	414, 1259, 1573, 2065
MspR9I	CCNGG	8 cut(s)	18, 323, 684, 825, 1194, 1245, 1573, 1574
MvaI	CCWGG	6 cut(s)	18, 323, 684, 825, 1194, 1245
MwoI	GCNNNNNNNGC	7 cut(s)	269, 323, 332, 341, 657, 1189, 1832
NciI	CCSGG	2 cut(s)	1573, 1574
NlaIV	GGNNCC	7 cut(s)	22, 474, 699, 759, 819, 1137, 1467
NmeAIII	GCCGAG	2 cut(s)	308, 370
NmuCI	GTSAC	6 cut(s)	418, 1628, 1636, 1796, 1828, 2011
NsiI	ATGCAT	1 cut(s)	1855
NspI	RCATGY	2 cut(s)	1857, 2002
NspV	TTCGAA	1 cut(s)	39
OliI	CACNNNNGTG	1 cut(s)	1690
PaeI	GCATGC	1 cut(s)	1857
PagI	TCATGA	1 cut(s)	517
PfeI	GAWTC	8 cut(s)	169, 187, 241, 361, 1276, 1369, 1440, 1948
PflFI	GACNNNGTC	1 cut(s)	1634
PkrI	GCNGC	3 cut(s)	1537, 1618, 1621
PleI	GAGTC	2 cut(s)	1039, 1678
PpsI	GAGTC	2 cut(s)	1039, 1678
PshBI	ATTAAT	1 cut(s)	807
Psp6I	CCWGG	6 cut(s)	16, 321, 682, 823, 1192, 1243
PspEI	GGTNACC	1 cut(s)	1628
PspFI	CCCAGC	2 cut(s)	263, 1178
PspGI	CCWGG	6 cut(s)	16, 321, 682, 823, 1192, 1243
PspN4I	GGNNCC	7 cut(s)	22, 474, 699, 759, 819, 1137, 1467
PspPI	GGNCC	3 cut(s)	21, 686, 1465
PstI	CTGCAG	2 cut(s)	1540, 1837
PstNI	CAGNNNCTG	1 cut(s)	253
PsuI	RGATCY	3 cut(s)	472, 524, 1401
PsyI	GACNNNGTC	1 cut(s)	1634
RsaI	GTAC	7 cut(s)	152, 158, 251, 450, 679, 1386, 1581
RsaNI	GTAC	7 cut(s)	151, 157, 250, 449, 678, 1385, 1580
RseI	CAYNNNNRTG	2 cut(s)	1690, 1875
SaqAI	TTAA	3 cut(s)	557, 807, 876
SatI	GCNGC	3 cut(s)	1536, 1617, 1620
Sau96I	GGNCC	3 cut(s)	21, 686, 1465
ScaI	AGTACT	1 cut(s)	1386
SchI	GAGTC	2 cut(s)	1039, 1679
ScrFI	CCNGG	8 cut(s)	18, 323, 684, 825, 1194, 1245, 1573, 1574
SduI	GDGCHC	1 cut(s)	1378
SfaNI	GCATC	5 cut(s)	95, 1160, 1254, 1405, 1788
SfcI	CTRYAG	2 cut(s)	1536, 1833
SfuI	TTCGAA	1 cut(s)	39
SinI	GGWCC	3 cut(s)	21, 686, 1465
SmaI	CCCGGG	1 cut(s)	1574
SmiMI	CAYNNNNRTG	2 cut(s)	1690, 1875
SmlI	CTYRAG	1 cut(s)	1667
SmoI	CTYRAG	1 cut(s)	1667
SpeI	ACTAGT	1 cut(s)	422
SphI	GCATGC	1 cut(s)	1857
Sse9I	AATT	9 cut(s)	431, 453, 634, 643, 741, 913, 971, 1107, 1702
SsiI	CCGC	3 cut(s)	326, 1620, 2002
SspI	AATATT	1 cut(s)	1027
SspMI	CTAG	3 cut(s)	102, 423, 1049
StyD4I	CCNGG	8 cut(s)	16, 321, 682, 823, 1192, 1243, 1571, 1572
TaaI	ACNGT	3 cut(s)	801, 1291, 1689
TaiI	ACGT	2 cut(s)	313, 574
TaqII	GACCGA	2 cut(s)	38, 539
TasI	AATT	9 cut(s)	431, 453, 634, 643, 741, 913, 971, 1107, 1702
TatI	WGTACW	4 cut(s)	150, 156, 1384, 1579
TauI	GCSGC	1 cut(s)	1622
TfiI	GAWTC	8 cut(s)	169, 187, 241, 361, 1276, 1369, 1440, 1948
Tru1I	TTAA	3 cut(s)	557, 807, 876
Tru9I	TTAA	3 cut(s)	557, 807, 876
TscAI	CASTG	4 cut(s)	153, 739, 1467, 1694
TseFI	GTSAC	6 cut(s)	418, 1628, 1636, 1796, 1828, 2011
TseI	GCWGC	2 cut(s)	1535, 1616
Tsp45I	GTSAC	6 cut(s)	418, 1628, 1636, 1796, 1828, 2011
TspDTI	ATGAA	7 cut(s)	58, 525, 825, 1029, 1611, 1739, 1910
TspGWI	ACGGA	3 cut(s)	1774, 1815, 1914
TspMI	CCCGGG	1 cut(s)	1572
TspRI	CASTG	4 cut(s)	153, 739, 1467, 1694
Tth111I	GACNNNGTC	1 cut(s)	1634
VpaK11BI	GGWCC	3 cut(s)	21, 686, 1465
VspI	ATTAAT	1 cut(s)	807
XagI	CCTNNNNNAGG	1 cut(s)	1248
XapI	RAATTY	4 cut(s)	431, 741, 913, 1702
XbaI	TCTAGA	1 cut(s)	1048
XceI	RCATGY	2 cut(s)	1857, 2002
XmaI	CCCGGG	1 cut(s)	1572
XmiI	GTMKAC	2 cut(s)	1653, 1884
XspI	CTAG	3 cut(s)	102, 423, 1049
ZrmI	AGTACT	1 cut(s)	1386
Zsp2I	ATGCAT	1 cut(s)	1855

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.