Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005801

Physical Location & Seq

Genomic Coordinates Reverse (-)

43778858 .. 43791594

Gene Structure 12737 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1305600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2061 bp

ATGTCGCAGTTCTGGAAACCAGGGACCGAGAAGCCCCGCCTCGTCGACGACGAAGAGGGCGGCGTCCTTTTCTTCAATTCCCCTTCTGGGTACGCGAACATAGAGCGGCAGAGGCAAAAGCTGCCCGTGTTCCGGTACCGCACAGCCATTCTTTACCTGGTGGAGACTCACGCCACCACCATCGTAGTCGGCGAAACGGGCAGCGGAAAAACCACCCAAATTCCTCAGTACCTCAAAGAAGCCGGTTGGGCTGACGGAGGGCGGGTTATAGCGTGCACCCAGCCAAGGAGGCTGGCTGTCCAGGCAGTTGCTGCAAGGGTCGCGGAAGAAATGGGGGTCAAGCTTGGGGAGGAAGTTGGCTACACTATAAGATTTGAAGATGGTGTAACTATGATAAAGTTTTTGACTGATGGTGTGCTACTCAGGGAAATGATGGATGATCCTCTTTTGACTAAATATAGCATCATAATGGTTGATGAGGCTCATGAAAGGTCTATCGCAACTGACATTTTATTGGGTCTCCTGAAAAAGATCCAACGACGCCGACCTGAGCTGCGTCTGATTATCTCATCTGCCACAATTCAAGCAAAGTCGATGTCTGATTTCTTTCAGCCCAGAAAAAGTCGTCGCAGACCAGACGGAGAGGATCTTGGACCAAATATGGAGCCTGCAGTCCTATCAGTTGAGGGTAGAGGGTTTAATGTACAAATTCATTATGTGGAGGAGCCTGTCTCTGACTATCTTCGGGCTGCTGTGTCAACTGTATTATTGATCCATGACCAGGAACCAATGGGAGATATTCTGGTTTTTCTTACTGGTCAAGATGATATTGATGCTGCTGTACAGCTGATTACTGAAGAAGCTCAAAACCGCGGAAAGAACTCATCAGGATTGATGCTTGTGCCGCTCTACTCAGGACTTCCACGTGCTGAGCAGGAGCTGGTGTTCACTCCTACCCCTAGAGGGAAGAGGAAAGTAGTGATATCAACCAATATAGCAGAGACGTCAATAACTTTGGAGGGCGTTGTCTATGTTGTTGACAGTGGGTTCTCGAAACAGCGTTTCTACAACCCGATAACAGATATTGAAAATCTGGTGGTGGCACCAATATCCAAGGCATCTGCTAGACAAAGGACTGGTAGGGCTGGAAGAGTTCGGCCTGGGAAATGTTACAGGCTATATACGGAAGAGTATTTTGCAAAGGAAATGTCTGATGAGGGGATTCCAGAGATACAGAGGTCAAATCTTGTGTCCTGTGTGATTCAGTTAAAAGCCTTGGGAATAGATAATATCCTGGGTTTCGATTGGCCAGCATCTCCTTCTTCCGAAGCAATGGTTCGAGCGCTTGAAGTGCTTTATTCACTTGGAGTCCTAGATGATGATGCAAAACTTACTTCGCCAACTGGATTTCAAATTGCTGAGTTTCCATTGGAACCAATGATTGCAAAAATGATATTATCTTCAGGTGAACTGGGGTGTTCAGAAGAGATAATTACCATTGCTGCTGTTCTCTCCATTCAAACTATCTGGGTTTCGGGTAGGGGATCGCAGAAGGAACTGGATGAAGCCAAGTTGAATTTTGCGGCTGCTGAGGGCGACCACGTGAGTTTCCTAAATGTCTACAAAGGATTTCTTCAGTCTGGTAAATCACCGCAGTGGTGCCATAGGAACTTTGTAAACTACCAAGCCATGAAAAAGGTTATTGAAATTAGAGGACAACTCAGAAGAACGGCTCAGAGATTAGGCGTTGTCTTGATATCTTGTGAAAGAGACACAGTGGTAGTGAGGAAGGCAATTACTAGTGGGTTTTTCGCTAATGCATGTCGATTAGATGCATCAAGTCACGACGGCAAGTACAAGACTATTAGAGGTTCTCAAGAAGTTTATATTCACCCATCATCCGTGTTATTCAGAGTTAATCCCAAGTGGGTTCTGTACCATTCCCTTGTCTCAACCGACAAGCAGTACATGCGGAATGTGATTTCGATAGATCCTTCTTGGCTCACAGAGGCAGCTCCACATTTTTACCAGCACCAGCGACTCAACTCGATGGTTCGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030054 GO:0032991 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0055044 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_22 HA2 Helicase_C OB_NTP_bind

Protein Analysis

687

Amino Acids

76.67

Weight (kDa)

7.61

Isoelectric Point (pI)

45.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_22	PF13401	58 - 179	2.1e-06	AAA domain
Helicase_C	PF00271	257 - 385	3.1e-12	Helicase conserved C-terminal domain
WHD_HA2	PF04408	448 - 477	3.4e-06	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	478 - 536	7.4e-10	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	595 - 674	4.6e-24	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005801)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18465
fragaria_vesca	FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450
malus_domestica	MD05G1305600.v1.1
prunus_persica	Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1
pyrus_communis	pycom05g28290
rosa_chinensis	RchiOBHm_Chr2g0158101 RchiOBHm_Chr5g0009991
rosa_laevigata	RLG00000031717
rosa_multiflora	Rmu_sc0001648.1_g000001 Rmu_sc0002275.1_g000005
rosa_roxburghii	Rroxscaffold_1G00066170
rosa_rugosa	Rorug04G0448300 Rorug04G0448400 Rorug04G0448500
rosa_samantha	Rh5AG079200 Rh5BG074000 Rh5CG086600 Rh5DG074700
rosa_wichuraiana	Rw5G007330 Rw7G028410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1007
Acc65I	GGTACC	1 cut(s)	135
AccB1I	GGYRCC	3 cut(s)	135, 1102, 1659
AccBSI	CCGCTC	2 cut(s)	106, 907
AccI	GTMKAC	2 cut(s)	45, 1620
AccII	CGCG	3 cut(s)	95, 323, 873
AclWI	GGATC	6 cut(s)	434, 526, 654, 766, 1552, 1985
AcoI	YGGCCR	1 cut(s)	1307
AcsI	RAATTY	3 cut(s)	219, 708, 1576
AcuI	CTGAAG	3 cut(s)	876, 1446, 1619
AcvI	CACGTG	2 cut(s)	926, 1603
AcyI	GRCGYC	3 cut(s)	63, 541, 1004
AfaI	GTAC	8 cut(s)	92, 137, 230, 705, 843, 1856, 1937, 1967
AfeI	AGCGCT	1 cut(s)	1344
AfiI	CCNNNNNNNGG	5 cut(s)	87, 132, 285, 781, 963
AgsI	TTSAA	9 cut(s)	76, 377, 584, 1088, 1349, 1412, 1520, 1576, 1706
AhlI	ACTAGT	1 cut(s)	1799
AjnI	CCWGG	6 cut(s)	19, 156, 300, 780, 1159, 1293
AleI	CACNNNNGTG	1 cut(s)	1654
AloI	GAACNNNNNNTCC	2 cut(s)	929, 961
AluBI	AGCT	7 cut(s)	121, 343, 553, 847, 863, 940, 2015
AluI	AGCT	7 cut(s)	121, 343, 553, 847, 863, 940, 2015
Alw21I	GWGCWC	1 cut(s)	278
Alw26I	GTCTC	6 cut(s)	158, 524, 736, 995, 1764, 1954
Alw44I	GTGCAC	1 cut(s)	274
AlwI	GGATC	6 cut(s)	434, 526, 654, 766, 1552, 1985
AlwNI	CAGNNNCTG	2 cut(s)	311, 940
Aor51HI	AGCGCT	1 cut(s)	1344
AoxI	GGCC	2 cut(s)	1157, 1307
ApaLI	GTGCAC	1 cut(s)	274
ApeKI	GCWGC	9 cut(s)	121, 201, 311, 553, 749, 836, 1502, 1586, 2012
ApoI	RAATTY	3 cut(s)	219, 708, 1576
ArsI	GACNNNNNNTTYG	6 cut(s)	581, 613, 1193, 1225, 1235, 1267
Asp718I	GGTACC	1 cut(s)	135
AspLEI	GCGC	1 cut(s)	1345
AspS9I	GGNCC	2 cut(s)	24, 653
AsuHPI	GGTGA	3 cut(s)	1478, 1641, 1883
AvaII	GGWCC	2 cut(s)	24, 653
BaeGI	GKGCMC	1 cut(s)	278
BaeI	ACNNNNGTAYC	2 cut(s)	1223, 1256
BalI	TGGCCA	1 cut(s)	1309
BanI	GGYRCC	3 cut(s)	135, 1102, 1659
BbrPI	CACGTG	2 cut(s)	926, 1603
Bbv12I	GWGCWC	1 cut(s)	278
BbvCI	CCTCAGC	1 cut(s)	1590
BbvI	GCAGC	9 cut(s)	108, 213, 298, 540, 736, 823, 1489, 1573, 2024
BccI	CCATC	6 cut(s)	188, 374, 404, 427, 1903, 2044
BceAI	ACGGC	2 cut(s)	1746, 1864
BciT130I	CCWGG	6 cut(s)	21, 158, 302, 782, 1161, 1295
BcoDI	GTCTC	6 cut(s)	158, 524, 736, 995, 1764, 1954
BcuI	ACTAGT	1 cut(s)	1799
BfaI	CTAG	4 cut(s)	960, 1125, 1373, 1800
BfmI	CTRYAG	1 cut(s)	669
BfoI	RGCGCY	1 cut(s)	1346
BglI	GCCNNNNNGGC	2 cut(s)	248, 289
BlpI	GCTNAGC	1 cut(s)	930
Bme1390I	CCNGG	6 cut(s)	21, 158, 302, 782, 1161, 1295
Bme18I	GGWCC	2 cut(s)	24, 653
BmgT120I	GGNCC	2 cut(s)	24, 653
BmiI	GGNNCC	8 cut(s)	25, 137, 666, 726, 786, 1104, 1434, 1661
BmrFI	CCNGG	6 cut(s)	21, 158, 302, 782, 1161, 1295
BmrI	ACTGGG	1 cut(s)	1481
BmsI	GCATC	8 cut(s)	471, 823, 885, 1127, 1322, 1372, 1822, 1844
BmuI	ACTGGG	1 cut(s)	1481
BplI	GAGNNNNNCTC	4 cut(s)	716, 748, 1704, 1736
Bpu10I	CCTNAGC	2 cut(s)	549, 1590
Bpu1102I	GCTNAGC	1 cut(s)	930
BpuEI	CTTGAG	1 cut(s)	1860
BsaAI	YACGTR	2 cut(s)	926, 1603
BsaHI	GRCGYC	3 cut(s)	63, 541, 1004
BsaI	GGTCTC	1 cut(s)	524
BsaJI	CCNNGG	7 cut(s)	20, 284, 871, 1113, 1160, 1275, 1294
BsaWI	WCCGGW	1 cut(s)	132
BsaXI	ACNNNNNCTCC	4 cut(s)	249, 279, 929, 959
Bsc4I	CCNNNNNNNGG	5 cut(s)	87, 132, 285, 781, 963
Bse118I	RCCGGY	1 cut(s)	242
Bse1I	ACTGG	5 cut(s)	820, 1141, 1408, 1476, 1563
Bse3DI	GCAATG	2 cut(s)	1338, 1497
BseBI	CCWGG	6 cut(s)	21, 158, 302, 782, 1161, 1295
BseDI	CCNNGG	7 cut(s)	20, 284, 871, 1113, 1160, 1275, 1294
BseGI	GGATG	3 cut(s)	442, 1567, 1898
BseLI	CCNNNNNNNGG	5 cut(s)	87, 132, 285, 781, 963
BseMI	GCAATG	2 cut(s)	1338, 1497
BseMII	CTCAG	9 cut(s)	239, 436, 540, 921, 927, 1410, 1581, 1735, 1748
BseNI	ACTGG	5 cut(s)	820, 1141, 1408, 1476, 1563
BseRI	GAGGAG	1 cut(s)	737
BseSI	GKGCMC	1 cut(s)	278
BseXI	GCAGC	9 cut(s)	108, 213, 298, 540, 736, 823, 1489, 1573, 2024
BseYI	CCCAGC	1 cut(s)	279
Bsh1236I	CGCG	3 cut(s)	95, 323, 873
BshFI	GGCC	2 cut(s)	1159, 1309
BshNI	GGYRCC	3 cut(s)	135, 1102, 1659
BsiHKAI	GWGCWC	1 cut(s)	278
BsiSI	CCGG	2 cut(s)	133, 243
BslFI	GGGAC	1 cut(s)	37
BslI	CCNNNNNNNGG	5 cut(s)	87, 132, 285, 781, 963
BsmAI	GTCTC	6 cut(s)	158, 524, 736, 995, 1764, 1954
BsmBI	CGTCTC	1 cut(s)	995
BsmFI	GGGAC	1 cut(s)	37
BsnI	GGCC	2 cut(s)	1159, 1309
Bso31I	GGTCTC	1 cut(s)	524
Bsp1286I	GDGCHC	1 cut(s)	278
Bsp1407I	TGTACA	2 cut(s)	703, 841
Bsp143I	GATC	6 cut(s)	439, 531, 646, 771, 1544, 1990
Bsp1720I	GCTNAGC	1 cut(s)	930
BspANI	GGCC	2 cut(s)	1159, 1309
BspCNI	CTCAG	9 cut(s)	238, 435, 541, 922, 926, 1411, 1582, 1734, 1747
BspFNI	CGCG	3 cut(s)	95, 323, 873
BspHI	TCATGA	1 cut(s)	484
BspLI	GGNNCC	8 cut(s)	25, 137, 666, 726, 786, 1104, 1434, 1661
BspMAI	CTGCAG	1 cut(s)	673
BspPI	GGATC	6 cut(s)	434, 526, 654, 766, 1552, 1985
BspT107I	GGYRCC	3 cut(s)	135, 1102, 1659
BspTNI	GGTCTC	1 cut(s)	524
BsrBI	CCGCTC	2 cut(s)	106, 907
BsrDI	GCAATG	2 cut(s)	1338, 1497
BsrFI	RCCGGY	1 cut(s)	242
BsrGI	TGTACA	2 cut(s)	703, 841
BsrI	ACTGG	5 cut(s)	820, 1141, 1408, 1476, 1563
BssAI	RCCGGY	1 cut(s)	242
BssECI	CCNNGG	7 cut(s)	20, 284, 871, 1113, 1160, 1275, 1294
BssMI	GATC	6 cut(s)	439, 531, 646, 771, 1544, 1990
BssNI	GRCGYC	3 cut(s)	63, 541, 1004
BssT1I	CCWWGG	3 cut(s)	284, 1113, 1275
Bst2UI	CCWGG	6 cut(s)	21, 158, 302, 782, 1161, 1295
Bst4CI	ACNGT	3 cut(s)	763, 1043, 1777
Bst6I	CTCTTC	5 cut(s)	48, 962, 1144, 1182, 1479
BstACI	GRCGYC	3 cut(s)	63, 541, 1004
BstAPI	GCANNNNNTGC	3 cut(s)	121, 311, 1969
BstAUI	TGTACA	2 cut(s)	703, 841
BstBAI	YACGTR	2 cut(s)	926, 1603
BstC8I	GCNNGC	4 cut(s)	274, 294, 669, 1311
BstDEI	CTNAG	9 cut(s)	225, 422, 549, 913, 930, 1419, 1590, 1721, 1734
BstDSI	CCRYGG	1 cut(s)	871
BstF5I	GGATG	3 cut(s)	442, 1567, 1898
BstFNI	CGCG	3 cut(s)	95, 323, 873
BstH2I	RGCGCY	1 cut(s)	1346
BstHHI	GCGC	1 cut(s)	1345
BstKTI	GATC	6 cut(s)	442, 534, 649, 774, 1547, 1993
BstMAI	GTCTC	6 cut(s)	158, 524, 736, 995, 1764, 1954
BstMBI	GATC	6 cut(s)	439, 531, 646, 771, 1544, 1990
BstNI	CCWGG	6 cut(s)	21, 158, 302, 782, 1161, 1295
BstNSI	RCATGY	2 cut(s)	1824, 1972
BstSCI	CCNGG	6 cut(s)	19, 156, 300, 780, 1159, 1293
BstSFI	CTRYAG	1 cut(s)	669
BstSLI	GKGCMC	1 cut(s)	278
BstUI	CGCG	3 cut(s)	95, 323, 873
BstV1I	GCAGC	9 cut(s)	108, 213, 298, 540, 736, 823, 1489, 1573, 2024
BstX2I	RGATCY	3 cut(s)	531, 646, 1990
BstYI	RGATCY	3 cut(s)	531, 646, 1990
BsuRI	GGCC	2 cut(s)	1159, 1309
BtgI	CCRYGG	1 cut(s)	871
BtsCI	GGATG	3 cut(s)	442, 1567, 1898
BtsI	GCAGTG	1 cut(s)	1661
BtsIMutI	CAGTG	3 cut(s)	1048, 1661, 1782
Cac8I	GCNNGC	4 cut(s)	274, 294, 669, 1311
CaiI	CAGNNNCTG	2 cut(s)	311, 940
CciI	TCATGA	1 cut(s)	484
CfoI	GCGC	1 cut(s)	1345
Cfr10I	RCCGGY	1 cut(s)	242
Cfr13I	GGNCC	2 cut(s)	24, 653
Cfr42I	CCGCGG	1 cut(s)	874
CseI	GACGC	3 cut(s)	52, 545, 549
CsiI	ACCWGGT	1 cut(s)	156
Csp6I	GTAC	8 cut(s)	91, 136, 229, 704, 842, 1855, 1936, 1966
CviAII	CATG	5 cut(s)	485, 776, 1690, 1821, 1969
CviQI	GTAC	8 cut(s)	91, 136, 229, 704, 842, 1855, 1936, 1966
DdeI	CTNAG	9 cut(s)	225, 422, 549, 913, 930, 1419, 1590, 1721, 1734
DpnI	GATC	6 cut(s)	441, 533, 648, 773, 1546, 1992
DpnII	GATC	6 cut(s)	439, 531, 646, 771, 1544, 1990
EaeI	YGGCCR	1 cut(s)	1307
Eam1104I	CTCTTC	5 cut(s)	48, 962, 1144, 1182, 1479
EarI	CTCTTC	5 cut(s)	48, 962, 1144, 1182, 1479
Eco130I	CCWWGG	3 cut(s)	284, 1113, 1275
Eco31I	GGTCTC	1 cut(s)	524
Eco32I	GATATC	2 cut(s)	984, 1758
Eco47I	GGWCC	2 cut(s)	24, 653
Eco47III	AGCGCT	1 cut(s)	1344
Eco57I	CTGAAG	3 cut(s)	876, 1446, 1619
Eco72I	CACGTG	2 cut(s)	926, 1603
EcoRII	CCWGG	6 cut(s)	19, 156, 300, 780, 1159, 1293
EcoRV	GATATC	2 cut(s)	984, 1758
EcoT14I	CCWWGG	3 cut(s)	284, 1113, 1275
EcoT22I	ATGCAT	2 cut(s)	1822, 1837
ErhI	CCWWGG	3 cut(s)	284, 1113, 1275
Esp3I	CGTCTC	1 cut(s)	995
FaeI	CATG	5 cut(s)	488, 779, 1693, 1824, 1972
FalI	AAGNNNNNCTT	2 cut(s)	1617, 1649
FaqI	GGGAC	1 cut(s)	37
FatI	CATG	5 cut(s)	484, 775, 1689, 1820, 1968
FauI	CCCGC	2 cut(s)	44, 255
FblI	GTMKAC	2 cut(s)	45, 1620
FokI	GGATG	3 cut(s)	449, 1574, 1885
FspBI	CTAG	4 cut(s)	960, 1125, 1373, 1800
GlaI	GCGC	1 cut(s)	1344
GsaI	CCCAGC	1 cut(s)	283
HaeII	RGCGCY	1 cut(s)	1346
HaeIII	GGCC	2 cut(s)	1159, 1309
HapII	CCGG	2 cut(s)	133, 243
HgaI	GACGC	3 cut(s)	52, 545, 549
HhaI	GCGC	1 cut(s)	1345
Hin1I	GRCGYC	3 cut(s)	63, 541, 1004
Hin1II	CATG	5 cut(s)	488, 779, 1693, 1824, 1972
Hin6I	GCGC	1 cut(s)	1343
HinP1I	GCGC	1 cut(s)	1343
HincII	GTYRAC	3 cut(s)	46, 759, 1039
HindII	GTYRAC	3 cut(s)	46, 759, 1039
HindIII	AAGCTT	1 cut(s)	341
HinfI	GANTC	5 cut(s)	166, 1222, 1261, 1368, 2040
HpaII	CCGG	2 cut(s)	133, 243
HphI	GGTGA	3 cut(s)	1478, 1641, 1883
Hpy166II	GTNNAC	8 cut(s)	46, 276, 759, 948, 1039, 1469, 1621, 1678
Hpy188I	TCNGA	9 cut(s)	561, 601, 736, 1213, 1327, 1483, 1724, 1737, 1913
Hpy8I	GTNNAC	8 cut(s)	46, 276, 759, 948, 1039, 1469, 1621, 1678
Hpy99I	CGWCG	6 cut(s)	47, 50, 53, 543, 630, 1850
HpyAV	CCTTC	5 cut(s)	93, 1329, 1546, 1783, 2004
HpyCH4III	ACNGT	3 cut(s)	763, 1043, 1777
HpyCH4IV	ACGT	3 cut(s)	925, 1004, 1602
HpyCH4V	TGCA	8 cut(s)	276, 314, 671, 1199, 1385, 1445, 1820, 1835
HpyF3I	CTNAG	9 cut(s)	225, 422, 549, 913, 930, 1419, 1590, 1721, 1734
HpySE526I	ACGT	3 cut(s)	925, 1004, 1602
Hsp92I	GRCGYC	3 cut(s)	63, 541, 1004
Hsp92II	CATG	5 cut(s)	488, 779, 1693, 1824, 1972
HspAI	GCGC	1 cut(s)	1343
KpnI	GGTACC	1 cut(s)	139
KspI	CCGCGG	1 cut(s)	874
Kzo9I	GATC	6 cut(s)	439, 531, 646, 771, 1544, 1990
LmnI	GCTCC	4 cut(s)	664, 724, 937, 2020
Lsp1109I	GCAGC	9 cut(s)	108, 213, 298, 540, 736, 823, 1489, 1573, 2024
LweI	GCATC	8 cut(s)	471, 823, 885, 1127, 1322, 1372, 1822, 1844
MabI	ACCWGGT	1 cut(s)	156
MaeI	CTAG	4 cut(s)	960, 1125, 1373, 1800
MaeII	ACGT	3 cut(s)	925, 1004, 1602
MaeIII	GTNAC	3 cut(s)	385, 1169, 1841
MalI	GATC	6 cut(s)	441, 533, 648, 773, 1546, 1992
MbiI	CCGCTC	2 cut(s)	106, 907
MboI	GATC	6 cut(s)	439, 531, 646, 771, 1544, 1990
MflI	RGATCY	3 cut(s)	531, 646, 1990
MhlI	GDGCHC	1 cut(s)	278
MlsI	TGGCCA	1 cut(s)	1309
MluCI	AATT	9 cut(s)	76, 219, 579, 708, 1413, 1491, 1576, 1707, 1794
MluNI	TGGCCA	1 cut(s)	1309
MlyI	GAGTC	3 cut(s)	160, 1377, 2034
MmeI	TCCRAC	1 cut(s)	559
Mox20I	TGGCCA	1 cut(s)	1309
Mph1103I	ATGCAT	2 cut(s)	1822, 1837
MscI	TGGCCA	1 cut(s)	1309
MseI	TTAA	3 cut(s)	699, 1268, 1917
MslI	CAYNNNNRTG	2 cut(s)	467, 1654
Msp20I	TGGCCA	1 cut(s)	1309
MspA1I	CMGCKG	3 cut(s)	204, 847, 873
MspI	CCGG	2 cut(s)	133, 243
MspR9I	CCNGG	6 cut(s)	21, 158, 302, 782, 1161, 1295
MvaI	CCWGG	6 cut(s)	21, 158, 302, 782, 1161, 1295
MvnI	CGCG	3 cut(s)	95, 323, 873
NdeII	GATC	6 cut(s)	439, 531, 646, 771, 1544, 1990
NlaIII	CATG	5 cut(s)	488, 779, 1693, 1824, 1972
NlaIV	GGNNCC	8 cut(s)	25, 137, 666, 726, 786, 1104, 1434, 1661
NmuCI	GTSAC	1 cut(s)	1841
NsiI	ATGCAT	2 cut(s)	1822, 1837
NspI	RCATGY	2 cut(s)	1824, 1972
OliI	CACNNNNGTG	1 cut(s)	1654
PagI	TCATGA	1 cut(s)	484
PcsI	WCGNNNNNNNCGW	2 cut(s)	48, 189
PfeI	GAWTC	2 cut(s)	1222, 1261
PleI	GAGTC	3 cut(s)	160, 1376, 2034
PmaCI	CACGTG	2 cut(s)	926, 1603
PmlI	CACGTG	2 cut(s)	926, 1603
PpsI	GAGTC	3 cut(s)	160, 1376, 2034
Ppu21I	YACGTR	2 cut(s)	926, 1603
Psp6I	CCWGG	6 cut(s)	19, 156, 300, 780, 1159, 1293
PspCI	CACGTG	2 cut(s)	926, 1603
PspFI	CCCAGC	1 cut(s)	279
PspGI	CCWGG	6 cut(s)	19, 156, 300, 780, 1159, 1293
PspN4I	GGNNCC	8 cut(s)	25, 137, 666, 726, 786, 1104, 1434, 1661
PspPI	GGNCC	2 cut(s)	24, 653
PstI	CTGCAG	1 cut(s)	673
PstNI	CAGNNNCTG	2 cut(s)	311, 940
PsuI	RGATCY	3 cut(s)	531, 646, 1990
PvuII	CAGCTG	1 cut(s)	847
RsaI	GTAC	8 cut(s)	92, 137, 230, 705, 843, 1856, 1937, 1967
RsaNI	GTAC	8 cut(s)	91, 136, 229, 704, 842, 1855, 1936, 1966
RseI	CAYNNNNRTG	2 cut(s)	467, 1654
SacII	CCGCGG	1 cut(s)	874
SalI	GTCGAC	1 cut(s)	44
SaqAI	TTAA	3 cut(s)	699, 1268, 1917
Sau3AI	GATC	6 cut(s)	439, 531, 646, 771, 1544, 1990
Sau96I	GGNCC	2 cut(s)	24, 653
SchI	GAGTC	3 cut(s)	160, 1377, 2034
ScrFI	CCNGG	6 cut(s)	21, 158, 302, 782, 1161, 1295
SduI	GDGCHC	1 cut(s)	278
SexAI	ACCWGGT	1 cut(s)	156
SfaNI	GCATC	8 cut(s)	471, 823, 885, 1127, 1322, 1372, 1822, 1844
SfcI	CTRYAG	1 cut(s)	669
Sfr303I	CCGCGG	1 cut(s)	874
SgrBI	CCGCGG	1 cut(s)	874
SgrDI	CGTCGACG	1 cut(s)	44
SinI	GGWCC	2 cut(s)	24, 653
SmiMI	CAYNNNNRTG	2 cut(s)	467, 1654
SmlI	CTYRAG	1 cut(s)	1875
SmoI	CTYRAG	1 cut(s)	1875
SpeI	ACTAGT	1 cut(s)	1799
Sse9I	AATT	9 cut(s)	76, 219, 579, 708, 1413, 1491, 1576, 1707, 1794
SspMI	CTAG	4 cut(s)	960, 1125, 1373, 1800
StyD4I	CCNGG	6 cut(s)	19, 156, 300, 780, 1159, 1293
StyI	CCWWGG	3 cut(s)	284, 1113, 1275
TaaI	ACNGT	3 cut(s)	763, 1043, 1777
TaiI	ACGT	3 cut(s)	928, 1007, 1605
TaqI	TCGA	8 cut(s)	45, 593, 1052, 1302, 1339, 1825, 1985, 2048
TaqII	GACCGA	1 cut(s)	41
TasI	AATT	9 cut(s)	76, 219, 579, 708, 1413, 1491, 1576, 1707, 1794
TatI	WGTACW	4 cut(s)	703, 841, 1854, 1965
TauI	GCSGC	4 cut(s)	63, 109, 907, 1586
TfiI	GAWTC	2 cut(s)	1222, 1261
Tru1I	TTAA	3 cut(s)	699, 1268, 1917
Tru9I	TTAA	3 cut(s)	699, 1268, 1917
TscAI	CASTG	3 cut(s)	1048, 1661, 1782
TseFI	GTSAC	1 cut(s)	1841
TseI	GCWGC	9 cut(s)	121, 201, 311, 553, 749, 836, 1502, 1586, 2012
Tsp45I	GTSAC	1 cut(s)	1841
TspDTI	ATGAA	4 cut(s)	501, 701, 1578, 1706
TspGWI	ACGGA	4 cut(s)	270, 654, 1199, 1891
TspRI	CASTG	3 cut(s)	1048, 1661, 1782
VneI	GTGCAC	1 cut(s)	274
VpaK11BI	GGWCC	2 cut(s)	24, 653
XapI	RAATTY	3 cut(s)	219, 708, 1576
XceI	RCATGY	2 cut(s)	1824, 1972
XcmI	CCANNNNNNNNNTGG	1 cut(s)	154
XmiI	GTMKAC	2 cut(s)	45, 1620
XspI	CTAG	4 cut(s)	960, 1125, 1373, 1800
ZraI	GACGTC	1 cut(s)	1005
Zsp2I	ATGCAT	2 cut(s)	1822, 1837

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.