Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0005801

Physical Location & Seq

Genomic Coordinates Forward (+)

6509642 .. 6516960

Gene Structure 7319 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG074700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2079 bp

ATGTCACAGTTCTGGAAACCAGGGGCGGAGAAGCCTCGCCTCCTCGACGACGAAGAAGGCGGCGTCGTTTTCTTCCCCACCTCCTCCGGAGGAGGCTATGCGAATCTCGAGAGGCAGAGGCAGAGACTACCGGTGTTCAGGTACCGCACCGCCATTCTCTACTCCGTCGAGACTCGCGCCACCACCGTCGTCGTCGGCGAAACCGGCAGCGGCAAAACCACTCAGATTCCTCAGTATTTGAAAGAAGCAGGTTGGGCTGATGGTGGCCGTGTAATTGCTTGTACTCAGCCGAGAAGATTGGCTGTTCAGTCGGTTGCAGCACGGGTGGCTGAAGAAATGGGGGTCAAGCTTGGGGAGGAAGTTGGCTACACTATTCGATTTGAAGATGCTACAACACAGGGTGTGACTATGATAAAGTTTTTGACTGATGGTGTGTTACTCAGGGAAATGATGGACGATCCTCTTTTGACTAAATATAGTGTTGTAATGGTAGATGAAGCTCATGAAAGGTCTATCTCCACGGACATTTTATTGGGTCTCCTGAAAAAGATCCAAAAACGCCGGCCTGAGCTGCGCCTGATTATCTCATCTGCTACAATTCAAGCAAAGTCAATGGCTGCTTTCTTTCAGTCCGGCAAAAGGCGCCGCAGACCGGAAGGAGAAGAGCTTGGACCAATTATGGAGCCTGCAGTCCTGTCAGTTGAGGGTAGAGGGTTTAATGTACAAATTCATTACGTGGAGGAGCCTGTTGCTGACTATCTTCGGGCTGCAGTCTCAACGGTATTATTGATACATGACCAGGAACCAATGGGAGATATTCTGGTATTTCTTACTGGTCAAGATGATATCGATGCTGCTGTCCAACTTATTACAGAAGAGACCCAAAACCGTAGAAAGAACTCATCAGGACTGATGGTTTTGCCTCTCTATTCGGGACTTCCACGTGCTGACCAGGAGCTGGTGTTCAGTCCTACTCCTAGAGGGAAAAGGAAAGTAGTTATATCAACCAACATAGCAGAGACATCAATAACTTTAGAGGGCGTGGTCTATGTTGTTGACAGTGGGTTCTCAAAGCAACGGTTCTATAACCCGATATCAGATATTGAAAATCTGGTAGTGGTACCAATATCCAAGGCATCTGCTAGGCAAAGGACCGGTAGAGCTGGAAGAGTTCGGCCTGGGAAATGTTACAGGCTGTATACAGAAGAGTATTTTGTAAAGGAAATGTCTGCTGAGGGAATTCCAGAGATACAAAGATCAAATCTTGTTTCCTGTGTGATCCAGTTAAAAGCCTTGGGAATAGATAATATATTGGGCTTTGATTGGCCAGCATCTCCATCTTCTGAAGCAATGGTTCGAGCACTTGAAGTGCTTTATTCACTTGGGGTCCTGGATGACGATGCTAAACTTACTTCACCAACTGGATTTCAAGTTGCTGAGTTTCCATTGGATCCAATGATCTCAAAGATGATCTTATCATCTGGTGAACTGGGGTGTTCAGAAGAGATTATTACTATTGCGGCTGTTCTCTCTGTTCAATCTATCTGGGCTTCTGGTTGGGGATCACAAAAGGAACTTGATGAAGCCAAGTTGAATTTTGCTGCAGCTGAGGGTGACCATGTGAGTTTCCTAAATGTCTACAAAGCATTTCTTCAGTCTGGTAAATCTTCACAGTGGTGTCATAAGAACTTTGTAAACTACCAAGCCATGAAAAAGGTTCTTGAAATAAGGGAACAACTCAGAAGAACAGCTCGAAGATTAGGCATTGACTTGAAGTCTTGTGAAAGAGATACAGTGGTAGTGAGAAAGGCCATTACTTATGGATTTTTTGCTAATGCGTGTGTTTCAGAAGCATCAAGTCACGATGGAAAGTACAAAACTATCAGAGGTTCTCAAGAAGTTTATATTCACCCCTCATCTGTGCTATTCAGAGTTAATCCGAAGTGGGTGGTTTACCATTCTCTTGTCTCAACTGAAAAGCAATACATGCGGAATGTCATTTCGATAGATCCTTCTTGGCTCACAGAGGCAGCTCCAGATTTTTACCAAAAGCAGCGACCCAATCCTATGCTTCACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030054 GO:0032991 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0055044 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_22 HA2 Helicase_C OB_NTP_bind

Protein Analysis

692

Amino Acids

77.18

Weight (kDa)

7.99

Isoelectric Point (pI)

48.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_22	PF13401	58 - 186	5.6e-07	AAA domain
DEAD	PF00270	58 - 204	2.2e-06	DEAD/DEAH box helicase
Helicase_C	PF00271	263 - 391	1.3e-12	Helicase conserved C-terminal domain
WHD_HA2	PF04408	454 - 483	5.8e-06	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	484 - 542	5.1e-10	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	601 - 679	1.5e-22	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005801)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18465
fragaria_vesca	FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450
malus_domestica	MD05G1305600.v1.1
prunus_persica	Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1
pyrus_communis	pycom05g28290
rosa_chinensis	RchiOBHm_Chr2g0158101 RchiOBHm_Chr5g0009991
rosa_laevigata	RLG00000031717
rosa_multiflora	Rmu_sc0001648.1_g000001 Rmu_sc0002275.1_g000005
rosa_roxburghii	Rroxscaffold_1G00066170
rosa_rugosa	Rorug04G0448300 Rorug04G0448400 Rorug04G0448500
rosa_samantha	Rh5AG079200 Rh5BG074000 Rh5CG086600 Rh5DG074700
rosa_wichuraiana	Rw5G007330 Rw7G028410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	239
Acc65I	GGTACC	2 cut(s)	141, 1120
AccB1I	GGYRCC	3 cut(s)	141, 642, 1120
AccB7I	CCANNNNNTGG	1 cut(s)	958
AccI	GTMKAC	2 cut(s)	1199, 1638
AccII	CGCG	1 cut(s)	177
AccIII	TCCGGA	1 cut(s)	86
AciI	CCGC	8 cut(s)	26, 60, 145, 150, 210, 646, 1520, 1990
AclWI	GGATC	7 cut(s)	452, 544, 1273, 1445, 1458, 1570, 2003
AcoI	YGGCCR	2 cut(s)	265, 1325
AcsI	RAATTY	3 cut(s)	726, 1239, 1594
AcuI	CTGAAG	3 cut(s)	351, 1365, 1637
AcvI	CACGTG	1 cut(s)	944
AcyI	GRCGYC	2 cut(s)	63, 643
AdeI	CACNNNGTG	1 cut(s)	401
AfaI	GTAC	5 cut(s)	143, 283, 723, 1122, 1874
AfiI	CCNNNNNNNGG	3 cut(s)	639, 652, 958
AgeI	ACCGGT	2 cut(s)	130, 1154
AhdI	GACNNNNNGTC	1 cut(s)	1774
AjnI	CCWGG	5 cut(s)	19, 798, 951, 1177, 1389
AleI	CACNNNNGTG	1 cut(s)	1675
AloI	GAACNNNNNNTCC	2 cut(s)	947, 979
AluBI	AGCT	9 cut(s)	349, 500, 571, 667, 958, 1163, 1607, 1751, 2033
AluI	AGCT	9 cut(s)	349, 500, 571, 667, 958, 1163, 1607, 1751, 2033
Alw21I	GWGCWC	1 cut(s)	1363
Alw26I	GTCTC	7 cut(s)	118, 164, 542, 778, 872, 1013, 1972
AlwI	GGATC	7 cut(s)	452, 544, 1273, 1445, 1458, 1570, 2003
AlwNI	CAGNNNCTG	1 cut(s)	958
Ama87I	CYCGRG	1 cut(s)	107
Aor13HI	TCCGGA	1 cut(s)	86
AoxI	GGCC	5 cut(s)	265, 563, 1175, 1325, 1809
ApoI	RAATTY	3 cut(s)	726, 1239, 1594
AsiGI	ACCGGT	2 cut(s)	130, 1154
Asp718I	GGTACC	2 cut(s)	141, 1120
AspLEI	GCGC	3 cut(s)	179, 576, 645
AspS9I	GGNCC	3 cut(s)	671, 1152, 1387
AsuHPI	GGTGA	4 cut(s)	1407, 1496, 1625, 1901
AvaI	CYCGRG	1 cut(s)	107
AvaII	GGWCC	3 cut(s)	671, 1152, 1387
BaeI	ACNNNNGTAYC	2 cut(s)	1782, 1815
BalI	TGGCCA	1 cut(s)	1327
BamHI	GGATCC	1 cut(s)	1450
BanI	GGYRCC	3 cut(s)	141, 642, 1120
BbrPI	CACGTG	1 cut(s)	944
Bbv12I	GWGCWC	1 cut(s)	1363
BbvCI	CCTCAGC	2 cut(s)	1233, 1608
BccI	CCATC	6 cut(s)	254, 422, 445, 907, 1345, 1859
BceAI	ACGGC	1 cut(s)	252
BciT130I	CCWGG	5 cut(s)	21, 800, 953, 1179, 1391
BcoDI	GTCTC	7 cut(s)	118, 164, 542, 778, 872, 1013, 1972
BfaI	CTAG	3 cut(s)	978, 1143, 2077
BfmI	CTRYAG	3 cut(s)	687, 768, 1602
BfoI	RGCGCY	1 cut(s)	646
BfuAI	ACCTGC	1 cut(s)	239
Bme1390I	CCNGG	5 cut(s)	21, 800, 953, 1179, 1391
Bme18I	GGWCC	3 cut(s)	671, 1152, 1387
BmeRI	GACNNNNNGTC	1 cut(s)	1774
BmeT110I	CYCGRG	1 cut(s)	107
BmgT120I	GGNCC	3 cut(s)	671, 1152, 1387
BmiI	GGNNCC	8 cut(s)	143, 644, 684, 744, 804, 1122, 1388, 1452
BmrFI	CCNGG	5 cut(s)	21, 800, 953, 1179, 1391
BmrI	ACTGGG	1 cut(s)	1499
BmsI	GCATC	6 cut(s)	376, 841, 1145, 1340, 1390, 1862
BmuI	ACTGGG	1 cut(s)	1499
BpmI	CTGGAG	1 cut(s)	2019
Bpu10I	CCTNAGC	3 cut(s)	567, 1233, 1608
BpuEI	CTTGAG	1 cut(s)	1878
Bsa29I	ATCGAT	1 cut(s)	849
BsaAI	YACGTR	2 cut(s)	736, 944
BsaHI	GRCGYC	2 cut(s)	63, 643
BsaI	GGTCTC	2 cut(s)	542, 872
BsaJI	CCNNGG	5 cut(s)	20, 519, 1131, 1178, 1293
BsaWI	WCCGGW	4 cut(s)	86, 130, 652, 1154
BsaXI	ACNNNNNCTCC	2 cut(s)	947, 977
Bsc4I	CCNNNNNNNGG	3 cut(s)	639, 652, 958
Bse118I	RCCGGY	4 cut(s)	130, 203, 561, 1154
Bse1I	ACTGG	4 cut(s)	838, 1282, 1426, 1494
Bse3DI	GCAATG	1 cut(s)	1356
BseAI	TCCGGA	1 cut(s)	86
BseBI	CCWGG	5 cut(s)	21, 800, 953, 1179, 1391
BseCI	ATCGAT	1 cut(s)	849
BseDI	CCNNGG	5 cut(s)	20, 519, 1131, 1178, 1293
BseGI	GGATG	1 cut(s)	1399
BseLI	CCNNNNNNNGG	3 cut(s)	639, 652, 958
BseMI	GCAATG	1 cut(s)	1356
BseMII	CTCAG	9 cut(s)	236, 245, 299, 454, 558, 1224, 1428, 1599, 1753
BseNI	ACTGG	4 cut(s)	838, 1282, 1426, 1494
BseRI	GAGGAG	4 cut(s)	32, 73, 105, 755
Bsh1236I	CGCG	1 cut(s)	177
BshFI	GGCC	5 cut(s)	267, 565, 1177, 1327, 1811
BshNI	GGYRCC	3 cut(s)	141, 642, 1120
BshTI	ACCGGT	2 cut(s)	130, 1154
BshVI	ATCGAT	1 cut(s)	849
BsiHKAI	GWGCWC	1 cut(s)	1363
BsiHKCI	CYCGRG	1 cut(s)	107
BsiSI	CCGG	7 cut(s)	87, 131, 204, 562, 633, 653, 1155
BslFI	GGGAC	1 cut(s)	948
BslI	CCNNNNNNNGG	3 cut(s)	639, 652, 958
BsmAI	GTCTC	7 cut(s)	118, 164, 542, 778, 872, 1013, 1972
BsmFI	GGGAC	1 cut(s)	948
BsnI	GGCC	5 cut(s)	267, 565, 1177, 1327, 1811
Bso31I	GGTCTC	2 cut(s)	542, 872
BsoBI	CYCGRG	1 cut(s)	107
Bsp1286I	GDGCHC	1 cut(s)	1363
Bsp13I	TCCGGA	1 cut(s)	86
Bsp1407I	TGTACA	1 cut(s)	721
Bsp143I	GATC	9 cut(s)	457, 549, 1256, 1278, 1450, 1458, 1470, 1562, 2008
BspACI	CCGC	8 cut(s)	26, 60, 145, 150, 210, 646, 1520, 1990
BspANI	GGCC	5 cut(s)	267, 565, 1177, 1327, 1811
BspCNI	CTCAG	9 cut(s)	235, 244, 298, 453, 559, 1225, 1429, 1600, 1752
BspDI	ATCGAT	1 cut(s)	849
BspEI	TCCGGA	1 cut(s)	86
BspFNI	CGCG	1 cut(s)	177
BspHI	TCATGA	1 cut(s)	502
BspLI	GGNNCC	8 cut(s)	143, 644, 684, 744, 804, 1122, 1388, 1452
BspMAI	CTGCAG	3 cut(s)	691, 772, 1606
BspMI	ACCTGC	1 cut(s)	239
BspPI	GGATC	7 cut(s)	452, 544, 1273, 1445, 1458, 1570, 2003
BspQI	GCTCTTC	1 cut(s)	657
BspT107I	GGYRCC	3 cut(s)	141, 642, 1120
BspTNI	GGTCTC	2 cut(s)	542, 872
BsrDI	GCAATG	1 cut(s)	1356
BsrFI	RCCGGY	4 cut(s)	130, 203, 561, 1154
BsrGI	TGTACA	1 cut(s)	721
BsrI	ACTGG	4 cut(s)	838, 1282, 1426, 1494
BssAI	RCCGGY	4 cut(s)	130, 203, 561, 1154
BssECI	CCNNGG	5 cut(s)	20, 519, 1131, 1178, 1293
BssMI	GATC	9 cut(s)	457, 549, 1256, 1278, 1450, 1458, 1470, 1562, 2008
BssNAI	GTATAC	1 cut(s)	1200
BssNI	GRCGYC	2 cut(s)	63, 643
BssT1I	CCWWGG	2 cut(s)	1131, 1293
Bst1107I	GTATAC	1 cut(s)	1200
Bst2UI	CCWGG	5 cut(s)	21, 800, 953, 1179, 1391
Bst4CI	ACNGT	8 cut(s)	9, 187, 781, 890, 1061, 1080, 1674, 1795
Bst6I	CTCTTC	5 cut(s)	657, 870, 1162, 1200, 1497
BstACI	GRCGYC	2 cut(s)	63, 643
BstAPI	GCANNNNNTGC	1 cut(s)	1987
BstAUI	TGTACA	1 cut(s)	721
BstBAI	YACGTR	2 cut(s)	736, 944
BstC8I	GCNNGC	3 cut(s)	563, 687, 1329
BstDEI	CTNAG	9 cut(s)	222, 231, 285, 440, 567, 1233, 1437, 1608, 1739
BstDSI	CCRYGG	1 cut(s)	519
BstEII	GGTNACC	1 cut(s)	1613
BstF5I	GGATG	1 cut(s)	1399
BstFNI	CGCG	1 cut(s)	177
BstH2I	RGCGCY	1 cut(s)	646
BstHHI	GCGC	3 cut(s)	179, 576, 645
BstKTI	GATC	9 cut(s)	460, 552, 1259, 1281, 1453, 1461, 1473, 1565, 2011
BstMAI	GTCTC	7 cut(s)	118, 164, 542, 778, 872, 1013, 1972
BstMBI	GATC	9 cut(s)	457, 549, 1256, 1278, 1450, 1458, 1470, 1562, 2008
BstMWI	GCNNNNNNNGC	6 cut(s)	204, 254, 326, 571, 642, 1987
BstNI	CCWGG	5 cut(s)	21, 800, 953, 1179, 1391
BstNSI	RCATGY	1 cut(s)	1990
BstPI	GGTNACC	1 cut(s)	1613
BstSCI	CCNGG	5 cut(s)	19, 798, 951, 1177, 1389
BstSFI	CTRYAG	3 cut(s)	687, 768, 1602
BstUI	CGCG	1 cut(s)	177
BstX2I	RGATCY	3 cut(s)	549, 1450, 2008
BstYI	RGATCY	3 cut(s)	549, 1450, 2008
BstZ17I	GTATAC	1 cut(s)	1200
Bsu15I	ATCGAT	1 cut(s)	849
BsuRI	GGCC	5 cut(s)	267, 565, 1177, 1327, 1811
BsuTUI	ATCGAT	1 cut(s)	849
BtgI	CCRYGG	1 cut(s)	519
BtsCI	GGATG	1 cut(s)	1399
BtsIMutI	CAGTG	3 cut(s)	1066, 1679, 1800
BveI	ACCTGC	1 cut(s)	239
Cac8I	GCNNGC	3 cut(s)	563, 687, 1329
CaiI	CAGNNNCTG	1 cut(s)	958
CciI	TCATGA	1 cut(s)	502
CfoI	GCGC	3 cut(s)	179, 576, 645
Cfr10I	RCCGGY	4 cut(s)	130, 203, 561, 1154
Cfr13I	GGNCC	3 cut(s)	671, 1152, 1387
ClaI	ATCGAT	1 cut(s)	849
CseI	GACGC	1 cut(s)	52
Csp6I	GTAC	5 cut(s)	142, 282, 722, 1121, 1873
CspAI	ACCGGT	2 cut(s)	130, 1154
CviAII	CATG	5 cut(s)	503, 794, 1619, 1708, 1987
CviQI	GTAC	5 cut(s)	142, 282, 722, 1121, 1873
DdeI	CTNAG	9 cut(s)	222, 231, 285, 440, 567, 1233, 1437, 1608, 1739
DinI	GGCGCC	1 cut(s)	644
DpnI	GATC	9 cut(s)	459, 551, 1258, 1280, 1452, 1460, 1472, 1564, 2010
DpnII	GATC	9 cut(s)	457, 549, 1256, 1278, 1450, 1458, 1470, 1562, 2008
DraIII	CACNNNGTG	1 cut(s)	401
DriI	GACNNNNNGTC	1 cut(s)	1774
EaeI	YGGCCR	2 cut(s)	265, 1325
Eam1104I	CTCTTC	5 cut(s)	657, 870, 1162, 1200, 1497
Eam1105I	GACNNNNNGTC	1 cut(s)	1774
EarI	CTCTTC	5 cut(s)	657, 870, 1162, 1200, 1497
EciI	GGCGGA	1 cut(s)	41
Eco130I	CCWWGG	2 cut(s)	1131, 1293
Eco31I	GGTCTC	2 cut(s)	542, 872
Eco32I	GATATC	2 cut(s)	847, 1095
Eco47I	GGWCC	3 cut(s)	671, 1152, 1387
Eco57I	CTGAAG	3 cut(s)	351, 1365, 1637
Eco72I	CACGTG	1 cut(s)	944
Eco88I	CYCGRG	1 cut(s)	107
Eco91I	GGTNACC	1 cut(s)	1613
EcoO109I	RGGNCCY	1 cut(s)	1387
EcoO65I	GGTNACC	1 cut(s)	1613
EcoRI	GAATTC	1 cut(s)	1239
EcoRII	CCWGG	5 cut(s)	19, 798, 951, 1177, 1389
EcoRV	GATATC	2 cut(s)	847, 1095
EcoT14I	CCWWGG	2 cut(s)	1131, 1293
EgeI	GGCGCC	1 cut(s)	644
EheI	GGCGCC	1 cut(s)	644
ErhI	CCWWGG	2 cut(s)	1131, 1293
FaeI	CATG	5 cut(s)	506, 797, 1622, 1711, 1990
FalI	AAGNNNNNCTT	4 cut(s)	1457, 1489, 1635, 1667
FaqI	GGGAC	1 cut(s)	948
FatI	CATG	5 cut(s)	502, 793, 1618, 1707, 1986
FblI	GTMKAC	2 cut(s)	1199, 1638
FokI	GGATG	1 cut(s)	1406
FspBI	CTAG	3 cut(s)	978, 1143, 2077
GlaI	GCGC	3 cut(s)	178, 575, 644
GsuI	CTGGAG	1 cut(s)	2019
HaeII	RGCGCY	1 cut(s)	646
HaeIII	GGCC	5 cut(s)	267, 565, 1177, 1327, 1811
HapII	CCGG	7 cut(s)	87, 131, 204, 562, 633, 653, 1155
HgaI	GACGC	1 cut(s)	52
HhaI	GCGC	3 cut(s)	179, 576, 645
Hin1I	GRCGYC	2 cut(s)	63, 643
Hin1II	CATG	5 cut(s)	506, 797, 1622, 1711, 1990
Hin6I	GCGC	3 cut(s)	177, 574, 643
HinP1I	GCGC	3 cut(s)	177, 574, 643
HincII	GTYRAC	1 cut(s)	1057
HindII	GTYRAC	1 cut(s)	1057
HindIII	AAGCTT	1 cut(s)	347
HinfI	GANTC	3 cut(s)	103, 172, 226
HpaII	CCGG	7 cut(s)	87, 131, 204, 562, 633, 653, 1155
HphI	GGTGA	4 cut(s)	1407, 1496, 1625, 1901
Hpy166II	GTNNAC	6 cut(s)	1057, 1200, 1487, 1639, 1696, 1954
Hpy188I	TCNGA	9 cut(s)	225, 1099, 1345, 1501, 1742, 1849, 1886, 1931, 1941
Hpy8I	GTNNAC	6 cut(s)	1057, 1200, 1487, 1639, 1696, 1954
Hpy99I	CGWCG	7 cut(s)	50, 53, 68, 170, 191, 194, 197
HpyAV	CCTTC	3 cut(s)	50, 650, 2022
HpyCH4III	ACNGT	8 cut(s)	9, 187, 781, 890, 1061, 1080, 1674, 1795
HpyCH4IV	ACGT	2 cut(s)	735, 943
HpyCH4V	TGCA	4 cut(s)	317, 689, 770, 1604
HpyF10VI	GCNNNNNNNGC	6 cut(s)	204, 254, 326, 571, 642, 1987
HpyF3I	CTNAG	9 cut(s)	222, 231, 285, 440, 567, 1233, 1437, 1608, 1739
HpySE526I	ACGT	2 cut(s)	735, 943
Hsp92I	GRCGYC	2 cut(s)	63, 643
Hsp92II	CATG	5 cut(s)	506, 797, 1622, 1711, 1990
HspAI	GCGC	3 cut(s)	177, 574, 643
KasI	GGCGCC	1 cut(s)	642
Kpn2I	TCCGGA	1 cut(s)	86
KpnI	GGTACC	2 cut(s)	145, 1124
KroI	GCCGGC	1 cut(s)	561
KroNI	GCCGGC	1 cut(s)	563
Kzo9I	GATC	9 cut(s)	457, 549, 1256, 1278, 1450, 1458, 1470, 1562, 2008
LguI	GCTCTTC	1 cut(s)	657
LmnI	GCTCC	4 cut(s)	682, 742, 955, 2038
LweI	GCATC	6 cut(s)	376, 841, 1145, 1340, 1390, 1862
MaeI	CTAG	3 cut(s)	978, 1143, 2077
MaeII	ACGT	2 cut(s)	735, 943
MaeIII	GTNAC	6 cut(s)	3, 403, 435, 1187, 1613, 1859
MalI	GATC	9 cut(s)	459, 551, 1258, 1280, 1452, 1460, 1472, 1564, 2010
MboI	GATC	9 cut(s)	457, 549, 1256, 1278, 1450, 1458, 1470, 1562, 2008
MflI	RGATCY	3 cut(s)	549, 1450, 2008
MhlI	GDGCHC	1 cut(s)	1363
MlsI	TGGCCA	1 cut(s)	1327
MluCI	AATT	6 cut(s)	273, 597, 675, 726, 1239, 1594
MluNI	TGGCCA	1 cut(s)	1327
Mly113I	GGCGCC	1 cut(s)	643
MlyI	GAGTC	1 cut(s)	166
MmeI	TCCRAC	1 cut(s)	886
Mox20I	TGGCCA	1 cut(s)	1327
MroI	TCCGGA	1 cut(s)	86
MroNI	GCCGGC	1 cut(s)	561
MscI	TGGCCA	1 cut(s)	1327
MseI	TTAA	3 cut(s)	717, 1286, 1935
MslI	CAYNNNNRTG	1 cut(s)	1675
Msp20I	TGGCCA	1 cut(s)	1327
MspA1I	CMGCKG	2 cut(s)	210, 1607
MspI	CCGG	7 cut(s)	87, 131, 204, 562, 633, 653, 1155
MspR9I	CCNGG	5 cut(s)	21, 800, 953, 1179, 1391
MvaI	CCWGG	5 cut(s)	21, 800, 953, 1179, 1391
MvnI	CGCG	1 cut(s)	177
MwoI	GCNNNNNNNGC	6 cut(s)	204, 254, 326, 571, 642, 1987
NaeI	GCCGGC	1 cut(s)	563
NarI	GGCGCC	1 cut(s)	643
NdeII	GATC	9 cut(s)	457, 549, 1256, 1278, 1450, 1458, 1470, 1562, 2008
NgoMIV	GCCGGC	1 cut(s)	561
NlaIII	CATG	5 cut(s)	506, 797, 1622, 1711, 1990
NlaIV	GGNNCC	8 cut(s)	143, 644, 684, 744, 804, 1122, 1388, 1452
NmeAIII	GCCGAG	1 cut(s)	315
NmuCI	GTSAC	4 cut(s)	3, 403, 1613, 1859
NspI	RCATGY	1 cut(s)	1990
OliI	CACNNNNGTG	1 cut(s)	1675
PaeR7I	CTCGAG	1 cut(s)	107
PagI	TCATGA	1 cut(s)	502
PciSI	GCTCTTC	1 cut(s)	657
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PdiI	GCCGGC	1 cut(s)	563
PfeI	GAWTC	2 cut(s)	103, 226
PflMI	CCANNNNNTGG	1 cut(s)	958
PfoI	TCCNGGA	1 cut(s)	1389
PinAI	ACCGGT	2 cut(s)	130, 1154
PleI	GAGTC	1 cut(s)	166
PluTI	GGCGCC	1 cut(s)	646
PmaCI	CACGTG	1 cut(s)	944
PmlI	CACGTG	1 cut(s)	944
PpsI	GAGTC	1 cut(s)	166
Ppu21I	YACGTR	2 cut(s)	736, 944
PpuMI	RGGWCCY	1 cut(s)	1387
Psp5II	RGGWCCY	1 cut(s)	1387
Psp6I	CCWGG	5 cut(s)	19, 798, 951, 1177, 1389
PspCI	CACGTG	1 cut(s)	944
PspEI	GGTNACC	1 cut(s)	1613
PspGI	CCWGG	5 cut(s)	19, 798, 951, 1177, 1389
PspN4I	GGNNCC	8 cut(s)	143, 644, 684, 744, 804, 1122, 1388, 1452
PspPI	GGNCC	3 cut(s)	671, 1152, 1387
PspPPI	RGGWCCY	1 cut(s)	1387
PstI	CTGCAG	3 cut(s)	691, 772, 1606
PstNI	CAGNNNCTG	1 cut(s)	958
PsuI	RGATCY	3 cut(s)	549, 1450, 2008
PvuII	CAGCTG	1 cut(s)	1607
RsaI	GTAC	5 cut(s)	143, 283, 723, 1122, 1874
RsaNI	GTAC	5 cut(s)	142, 282, 722, 1121, 1873
RseI	CAYNNNNRTG	1 cut(s)	1675
SapI	GCTCTTC	1 cut(s)	657
SaqAI	TTAA	3 cut(s)	717, 1286, 1935
Sau3AI	GATC	9 cut(s)	457, 549, 1256, 1278, 1450, 1458, 1470, 1562, 2008
Sau96I	GGNCC	3 cut(s)	671, 1152, 1387
SchI	GAGTC	1 cut(s)	166
ScrFI	CCNGG	5 cut(s)	21, 800, 953, 1179, 1391
SduI	GDGCHC	1 cut(s)	1363
SfaNI	GCATC	6 cut(s)	376, 841, 1145, 1340, 1390, 1862
SfcI	CTRYAG	3 cut(s)	687, 768, 1602
SfoI	GGCGCC	1 cut(s)	644
Sfr274I	CTCGAG	1 cut(s)	107
SinI	GGWCC	3 cut(s)	671, 1152, 1387
SlaI	CTCGAG	1 cut(s)	107
SmiMI	CAYNNNNRTG	1 cut(s)	1675
SmlI	CTYRAG	2 cut(s)	107, 1893
SmoI	CTYRAG	2 cut(s)	107, 1893
Sse9I	AATT	6 cut(s)	273, 597, 675, 726, 1239, 1594
SsiI	CCGC	8 cut(s)	26, 60, 145, 150, 210, 646, 1520, 1990
SspDI	GGCGCC	1 cut(s)	642
SspMI	CTAG	3 cut(s)	978, 1143, 2077
StyD4I	CCNGG	5 cut(s)	19, 798, 951, 1177, 1389
StyI	CCWWGG	2 cut(s)	1131, 1293
TaaI	ACNGT	8 cut(s)	9, 187, 781, 890, 1061, 1080, 1674, 1795
TaiI	ACGT	2 cut(s)	738, 946
TaqI	TCGA	8 cut(s)	45, 108, 168, 376, 849, 1357, 1753, 2003
TasI	AATT	6 cut(s)	273, 597, 675, 726, 1239, 1594
TatI	WGTACW	3 cut(s)	281, 721, 1872
TauI	GCSGC	4 cut(s)	63, 213, 648, 1523
TfiI	GAWTC	2 cut(s)	103, 226
Tru1I	TTAA	3 cut(s)	717, 1286, 1935
Tru9I	TTAA	3 cut(s)	717, 1286, 1935
TscAI	CASTG	3 cut(s)	1066, 1679, 1800
TseFI	GTSAC	4 cut(s)	3, 403, 1613, 1859
Tsp45I	GTSAC	4 cut(s)	3, 403, 1613, 1859
TspDTI	ATGAA	5 cut(s)	510, 519, 719, 1596, 1724
TspGWI	ACGGA	2 cut(s)	154, 536
TspRI	CASTG	3 cut(s)	1066, 1679, 1800
Van91I	CCANNNNNTGG	1 cut(s)	958
VpaK11BI	GGWCC	3 cut(s)	671, 1152, 1387
XapI	RAATTY	3 cut(s)	726, 1239, 1594
XceI	RCATGY	1 cut(s)	1990
XhoI	CTCGAG	1 cut(s)	107
XmiI	GTMKAC	2 cut(s)	1199, 1638
XspI	CTAG	3 cut(s)	978, 1143, 2077

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.