Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0005801

Physical Location & Seq

Genomic Coordinates Reverse (-)

29060021 .. 29066978

Gene Structure 6958 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g28290.9

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2160 bp

ATGGGTCCAGGTGTTGGAGCCCAACAGTTGGACTCTCTAAAACCCCAACGGAAGAAATCGCCGAGGAAAAAAGCAAGAGCCACAACAATGTCGCAGTTCTGGAAACCAGGGACCGAGAAGCCCCGCCTCGTCGACGACGAAGAGGGCGGTGTCCTTTTCTTCAATTCCCCTTCTGGGTACGCGAACATTGAGCGGCAGAGGCAAAGGCTGCCCGTGTTCCGGTACCGCACTGCCATTCTTTACCTGGTGGAGACTCACGCCACCACGATCATAGTCGGCGAAACGGGCAGCGGCAAAACTACCCAAATTCCTCAGTACCTCAAAGAAGCCGGTTGGGCTGACGGAGGGCGGGTTATAGCGTGCACCCAGCCAAGGAGGCTGGCTGTCCAGGCAGTTGCTGCAAGGGTCGCGGAAGAAATGGGGGTCAAGCTTGGGGAGGAAGTTGGCTACACTATAAGATTTGAAGATGTTACAACACAGGGTGTAACTATGATAAAGTTTTTGACTGATGGTGTGCTACTCAGGGAAATGATGGATGATCCTCTTTTGACTAAATATAGCGTCATAATGGTTGATGAGGCTCATGAAAGGTCTATCGCAACTGACATTTTATTGGGTCTCCTGAAAAAGATCCAACGACGCCGACCTGAGCTGCGTCTGGTTATCTCATCTGCCACAATTCAAGCAAAGTCGATGTCTGAATTCTTTCAGCCCAGAAAAAGTCGTCGCAGACCAGACGGAGAGGATCTTGGGCCAAATATGGAGCCTGCAGTCCTATCAGTTGAGGGTAGAGGGTTTAATGTACAAATTCATTATGTGGAGGAGCCTGTATCTGACTATCTTCGGGCTGCTGTGTCAACTGTATTATTGATCCATGACCAGGAACCAATGGGAGATATTCTGGTTTTTCTTACTGGTCAAGATGATATTGATGCTGCTGTACAGCTGATTACTGAAGAAGCTCAAAACCGCGGAAAGAACTCATCAGGATTGATGCTTGTGCCGCTCTACTCAGGACTTCCACGTGCTGAGCAGGAGCTGGTGTTCACTCCTACTCCCAGAGGGAAGAGGAAAGTAGTGATATCAACCAATATAGCAGAGACGTCAATAACTTTGGAGGGCGTTGTCTATGTTGTTGACAGTGGGTTCTCGAAGCAGCGTTTCTACAACCCGATAACAGATATTGAAAATCTGGTAGTGGCACCAATATCCAAGGCATCTGCTAGACAAAGAACTGGTAGGGCTGGAAGAGTTCGGCCTGGGAAATGTTACAGGCTATATACGGAAGAGTATTTTGCAAAGGAAATGTCTGATGAGGGGATTCCAGAGATACAGAGGTCAAATCTTGTGTCCTGTGTGATTCAGTTAAAAGCCTTGGGAATAGATAATATCCTGGGTTTTGATTGGCCAGCATCTCCTTCTTCCGAAGCAATGGTTCGAGCGCTTGAAGTGCTTTATTCACTTGGAGTCCTAGATGATGATGCAAAACTTACTTCGCCAACTGGATTTCAAATTGCTGAGTTTCCATTGGAACCAATGATTGCAAAAATGATATTATCTTCAGGTGAACTGGGGTGTTCAGAAGAGATAATTACCATTGCTGCCGTTCTCTCCATTCAAACTATCTGGGTTTCGGGTAGGGGATCGCAGAAGGAACTGGATGAAGCCAAGTTGAATTTTGCGGCTGCCGAGGGCGACCACGTGAGTTTCCTAAATGTCTACAAAGGATTTCTTCAGTCTGGTAAATCGCCACAGTGGTGCCATAAGAACTTTGTAAACTACCAAGCCATGAAAAAGGTTATTGAAATTAGAGGACAACTCAGAAGAACGGCTCAGAGATTAGGCATTGTCTTGAAATCTTGTGAAAGAGACACAGTGGTAGTGAGGAAGGCCATTACTAGTGGGTTTTTCGCTAATGCATGTCGATTAGATGCATCAAGTCACGACGGGAAGTACAAGACTATTAGAGGTTCTCAAGAAGTTTATATTCACCCATCATCCGTGTTATTCAGAGTTAATCCCAAGTCGGTTCTGTACCATTCTCTTGTCTCAACCGACAAGCAGTACATGCGGAATGTGATTTCGATAGATCCTTCTTGGCTCACAGAGGCAGCTCCACATTTTTACCAGCACCAGCGACTCAACTCGATGGTTCGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030054 GO:0032991 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0055044 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_22 HA2 Helicase_C OB_NTP_bind

Protein Analysis

720

Amino Acids

80.14

Weight (kDa)

8.93

Isoelectric Point (pI)

45.3

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005801)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18465
fragaria_vesca	FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450
malus_domestica	MD05G1305600.v1.1
prunus_persica	Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1
pyrus_communis	pycom05g28290
rosa_chinensis	RchiOBHm_Chr2g0158101 RchiOBHm_Chr5g0009991
rosa_laevigata	RLG00000031717
rosa_multiflora	Rmu_sc0001648.1_g000001 Rmu_sc0002275.1_g000005
rosa_roxburghii	Rroxscaffold_1G00066170
rosa_rugosa	Rorug04G0448300 Rorug04G0448400 Rorug04G0448500
rosa_samantha	Rh5AG079200 Rh5BG074000 Rh5CG086600 Rh5DG074700
rosa_wichuraiana	Rw5G007330 Rw7G028410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1106
Acc65I	GGTACC	1 cut(s)	222
AccB1I	GGYRCC	3 cut(s)	222, 1201, 1758
AccB7I	CCANNNNNTGG	2 cut(s)	14, 28
AccBSI	CCGCTC	2 cut(s)	193, 1006
AccI	GTMKAC	2 cut(s)	132, 1719
AccII	CGCG	3 cut(s)	182, 410, 972
AclWI	GGATC	6 cut(s)	533, 625, 753, 865, 1651, 2084
AcoI	YGGCCR	1 cut(s)	1406
AcsI	RAATTY	4 cut(s)	306, 701, 807, 1675
AcuI	CTGAAG	3 cut(s)	975, 1545, 1718
AcvI	CACGTG	2 cut(s)	1025, 1702
AcyI	GRCGYC	2 cut(s)	640, 1103
AdeI	CACNNNGTG	1 cut(s)	482
AfaI	GTAC	8 cut(s)	179, 224, 317, 804, 942, 1955, 2036, 2066
AfeI	AGCGCT	1 cut(s)	1443
AfiI	CCNNNNNNNGG	6 cut(s)	14, 28, 174, 219, 372, 880
AhlI	ACTAGT	1 cut(s)	1898
AjnI	CCWGG	7 cut(s)	7, 106, 243, 387, 879, 1258, 1392
AleI	CACNNNNGTG	1 cut(s)	1756
AloI	GAACNNNNNNTCC	2 cut(s)	1028, 1060
AluBI	AGCT	6 cut(s)	430, 652, 946, 962, 1039, 2114
AluI	AGCT	6 cut(s)	430, 652, 946, 962, 1039, 2114
Alw21I	GWGCWC	1 cut(s)	365
Alw26I	GTCTC	5 cut(s)	245, 623, 1094, 1863, 2053
Alw44I	GTGCAC	1 cut(s)	361
AlwI	GGATC	6 cut(s)	533, 625, 753, 865, 1651, 2084
AlwNI	CAGNNNCTG	2 cut(s)	398, 1039
Aor51HI	AGCGCT	1 cut(s)	1443
AoxI	GGCC	4 cut(s)	752, 1256, 1406, 1890
ApaLI	GTGCAC	1 cut(s)	361
ApoI	RAATTY	4 cut(s)	306, 701, 807, 1675
ArsI	GACNNNNNNTTYG	6 cut(s)	680, 712, 1292, 1324, 1334, 1366
Asp700I	GAANNNNTTC	1 cut(s)	705
Asp718I	GGTACC	1 cut(s)	222
AspLEI	GCGC	1 cut(s)	1444
AspS9I	GGNCC	3 cut(s)	5, 111, 752
AsuHPI	GGTGA	2 cut(s)	1577, 1982
AvaII	GGWCC	2 cut(s)	5, 111
BaeGI	GKGCMC	1 cut(s)	365
BaeI	ACNNNNGTAYC	2 cut(s)	1322, 1355
BalI	TGGCCA	1 cut(s)	1408
BanI	GGYRCC	3 cut(s)	222, 1201, 1758
BanII	GRGCYC	1 cut(s)	22
BbrPI	CACGTG	2 cut(s)	1025, 1702
Bbv12I	GWGCWC	1 cut(s)	365
BccI	CCATC	4 cut(s)	503, 526, 2002, 2143
BceAI	ACGGC	2 cut(s)	1589, 1845
BciT130I	CCWGG	7 cut(s)	9, 108, 245, 389, 881, 1260, 1394
BcoDI	GTCTC	5 cut(s)	245, 623, 1094, 1863, 2053
BcuI	ACTAGT	1 cut(s)	1898
BfaI	CTAG	3 cut(s)	1224, 1472, 1899
BfmI	CTRYAG	1 cut(s)	768
BfoI	RGCGCY	1 cut(s)	1445
BglI	GCCNNNNNGGC	2 cut(s)	335, 376
BlpI	GCTNAGC	1 cut(s)	1029
Bme1390I	CCNGG	7 cut(s)	9, 108, 245, 389, 881, 1260, 1394
Bme18I	GGWCC	2 cut(s)	5, 111
BmgT120I	GGNCC	3 cut(s)	5, 111, 752
BmrFI	CCNGG	7 cut(s)	9, 108, 245, 389, 881, 1260, 1394
BmrI	ACTGGG	1 cut(s)	1580
BmsI	GCATC	7 cut(s)	922, 984, 1226, 1421, 1471, 1921, 1943
BmuI	ACTGGG	1 cut(s)	1580
BplI	GAGNNNNNCTC	2 cut(s)	1803, 1835
Bpu10I	CCTNAGC	1 cut(s)	648
Bpu1102I	GCTNAGC	1 cut(s)	1029
BpuEI	CTTGAG	1 cut(s)	1959
BsaAI	YACGTR	2 cut(s)	1025, 1702
BsaHI	GRCGYC	2 cut(s)	640, 1103
BsaI	GGTCTC	1 cut(s)	623
BsaJI	CCNNGG	9 cut(s)	62, 107, 371, 970, 1212, 1259, 1374, 1393, 1689
BsaWI	WCCGGW	1 cut(s)	219
BsaXI	ACNNNNNCTCC	6 cut(s)	336, 366, 1028, 1039, 1058, 1069
Bsc4I	CCNNNNNNNGG	6 cut(s)	14, 28, 174, 219, 372, 880
Bse118I	RCCGGY	1 cut(s)	329
Bse1I	ACTGG	5 cut(s)	919, 1240, 1507, 1575, 1662
Bse3DI	GCAATG	2 cut(s)	1437, 1596
BseBI	CCWGG	7 cut(s)	9, 108, 245, 389, 881, 1260, 1394
BseDI	CCNNGG	9 cut(s)	62, 107, 371, 970, 1212, 1259, 1374, 1393, 1689
BseGI	GGATG	3 cut(s)	541, 1666, 1997
BseLI	CCNNNNNNNGG	6 cut(s)	14, 28, 174, 219, 372, 880
BseMI	GCAATG	2 cut(s)	1437, 1596
BseMII	CTCAG	8 cut(s)	326, 535, 639, 1020, 1026, 1509, 1834, 1847
BseNI	ACTGG	5 cut(s)	919, 1240, 1507, 1575, 1662
BseRI	GAGGAG	1 cut(s)	836
BseSI	GKGCMC	1 cut(s)	365
BseYI	CCCAGC	1 cut(s)	366
Bsh1236I	CGCG	3 cut(s)	182, 410, 972
BshFI	GGCC	4 cut(s)	754, 1258, 1408, 1892
BshNI	GGYRCC	3 cut(s)	222, 1201, 1758
BsiHKAI	GWGCWC	1 cut(s)	365
BsiSI	CCGG	2 cut(s)	220, 330
BslFI	GGGAC	1 cut(s)	124
BslI	CCNNNNNNNGG	6 cut(s)	14, 28, 174, 219, 372, 880
BsmAI	GTCTC	5 cut(s)	245, 623, 1094, 1863, 2053
BsmBI	CGTCTC	1 cut(s)	1094
BsmFI	GGGAC	1 cut(s)	124
BsnI	GGCC	4 cut(s)	754, 1258, 1408, 1892
Bso31I	GGTCTC	1 cut(s)	623
Bsp1286I	GDGCHC	2 cut(s)	22, 365
Bsp1407I	TGTACA	2 cut(s)	802, 940
Bsp143I	GATC	7 cut(s)	267, 538, 630, 745, 870, 1643, 2089
Bsp1720I	GCTNAGC	1 cut(s)	1029
BspANI	GGCC	4 cut(s)	754, 1258, 1408, 1892
BspCNI	CTCAG	8 cut(s)	325, 534, 640, 1021, 1025, 1510, 1833, 1846
BspFNI	CGCG	3 cut(s)	182, 410, 972
BspHI	TCATGA	1 cut(s)	583
BspMAI	CTGCAG	1 cut(s)	772
BspPI	GGATC	6 cut(s)	533, 625, 753, 865, 1651, 2084
BspT107I	GGYRCC	3 cut(s)	222, 1201, 1758
BspTNI	GGTCTC	1 cut(s)	623
BsrBI	CCGCTC	2 cut(s)	193, 1006
BsrDI	GCAATG	2 cut(s)	1437, 1596
BsrFI	RCCGGY	1 cut(s)	329
BsrGI	TGTACA	2 cut(s)	802, 940
BsrI	ACTGG	5 cut(s)	919, 1240, 1507, 1575, 1662
BssAI	RCCGGY	1 cut(s)	329
BssECI	CCNNGG	9 cut(s)	62, 107, 371, 970, 1212, 1259, 1374, 1393, 1689
BssMI	GATC	7 cut(s)	267, 538, 630, 745, 870, 1643, 2089
BssNI	GRCGYC	2 cut(s)	640, 1103
BssT1I	CCWWGG	3 cut(s)	371, 1212, 1374
Bst2UI	CCWGG	7 cut(s)	9, 108, 245, 389, 881, 1260, 1394
Bst4CI	ACNGT	5 cut(s)	27, 862, 1142, 1755, 1876
Bst6I	CTCTTC	5 cut(s)	135, 1061, 1243, 1281, 1578
BstACI	GRCGYC	2 cut(s)	640, 1103
BstAPI	GCANNNNNTGC	3 cut(s)	208, 398, 2068
BstAUI	TGTACA	2 cut(s)	802, 940
BstBAI	YACGTR	2 cut(s)	1025, 1702
BstC8I	GCNNGC	4 cut(s)	361, 381, 768, 1410
BstDEI	CTNAG	8 cut(s)	312, 521, 648, 1012, 1029, 1518, 1820, 1833
BstDSI	CCRYGG	1 cut(s)	970
BstF5I	GGATG	3 cut(s)	541, 1666, 1997
BstFNI	CGCG	3 cut(s)	182, 410, 972
BstH2I	RGCGCY	1 cut(s)	1445
BstHHI	GCGC	1 cut(s)	1444
BstKTI	GATC	7 cut(s)	270, 541, 633, 748, 873, 1646, 2092
BstMAI	GTCTC	5 cut(s)	245, 623, 1094, 1863, 2053
BstMBI	GATC	7 cut(s)	267, 538, 630, 745, 870, 1643, 2089
BstNI	CCWGG	7 cut(s)	9, 108, 245, 389, 881, 1260, 1394
BstNSI	RCATGY	2 cut(s)	1923, 2071
BstSCI	CCNGG	7 cut(s)	7, 106, 243, 387, 879, 1258, 1392
BstSFI	CTRYAG	1 cut(s)	768
BstSLI	GKGCMC	1 cut(s)	365
BstUI	CGCG	3 cut(s)	182, 410, 972
BstX2I	RGATCY	3 cut(s)	630, 745, 2089
BstYI	RGATCY	3 cut(s)	630, 745, 2089
BsuRI	GGCC	4 cut(s)	754, 1258, 1408, 1892
BtgI	CCRYGG	1 cut(s)	970
BtsCI	GGATG	3 cut(s)	541, 1666, 1997
BtsI	GCAGTG	1 cut(s)	228
BtsIMutI	CAGTG	4 cut(s)	228, 1147, 1760, 1881
Cac8I	GCNNGC	4 cut(s)	361, 381, 768, 1410
CaiI	CAGNNNCTG	2 cut(s)	398, 1039
CciI	TCATGA	1 cut(s)	583
CfoI	GCGC	1 cut(s)	1444
Cfr10I	RCCGGY	1 cut(s)	329
Cfr13I	GGNCC	3 cut(s)	5, 111, 752
Cfr42I	CCGCGG	1 cut(s)	973
CseI	GACGC	3 cut(s)	550, 644, 648
CsiI	ACCWGGT	1 cut(s)	243
Csp6I	GTAC	8 cut(s)	178, 223, 316, 803, 941, 1954, 2035, 2065
CviAII	CATG	5 cut(s)	584, 875, 1789, 1920, 2068
CviQI	GTAC	8 cut(s)	178, 223, 316, 803, 941, 1954, 2035, 2065
DdeI	CTNAG	8 cut(s)	312, 521, 648, 1012, 1029, 1518, 1820, 1833
DpnI	GATC	7 cut(s)	269, 540, 632, 747, 872, 1645, 2091
DpnII	GATC	7 cut(s)	267, 538, 630, 745, 870, 1643, 2089
DraIII	CACNNNGTG	1 cut(s)	482
EaeI	YGGCCR	1 cut(s)	1406
Eam1104I	CTCTTC	5 cut(s)	135, 1061, 1243, 1281, 1578
EarI	CTCTTC	5 cut(s)	135, 1061, 1243, 1281, 1578
Eco130I	CCWWGG	3 cut(s)	371, 1212, 1374
Eco24I	GRGCYC	1 cut(s)	22
Eco31I	GGTCTC	1 cut(s)	623
Eco32I	GATATC	1 cut(s)	1083
Eco47I	GGWCC	2 cut(s)	5, 111
Eco47III	AGCGCT	1 cut(s)	1443
Eco57I	CTGAAG	3 cut(s)	975, 1545, 1718
Eco72I	CACGTG	2 cut(s)	1025, 1702
EcoRI	GAATTC	1 cut(s)	701
EcoRII	CCWGG	7 cut(s)	7, 106, 243, 387, 879, 1258, 1392
EcoRV	GATATC	1 cut(s)	1083
EcoT14I	CCWWGG	3 cut(s)	371, 1212, 1374
EcoT22I	ATGCAT	2 cut(s)	1921, 1936
EcoT38I	GRGCYC	1 cut(s)	22
ErhI	CCWWGG	3 cut(s)	371, 1212, 1374
Esp3I	CGTCTC	1 cut(s)	1094
FaeI	CATG	5 cut(s)	587, 878, 1792, 1923, 2071
FalI	AAGNNNNNCTT	2 cut(s)	1716, 1748
FaqI	GGGAC	1 cut(s)	124
FatI	CATG	5 cut(s)	583, 874, 1788, 1919, 2067
FauI	CCCGC	2 cut(s)	131, 342
FblI	GTMKAC	2 cut(s)	132, 1719
FokI	GGATG	3 cut(s)	548, 1673, 1984
FriOI	GRGCYC	1 cut(s)	22
FspBI	CTAG	3 cut(s)	1224, 1472, 1899
GlaI	GCGC	1 cut(s)	1443
GsaI	CCCAGC	1 cut(s)	370
HaeII	RGCGCY	1 cut(s)	1445
HaeIII	GGCC	4 cut(s)	754, 1258, 1408, 1892
HapII	CCGG	2 cut(s)	220, 330
HgaI	GACGC	3 cut(s)	550, 644, 648
HhaI	GCGC	1 cut(s)	1444
Hin1I	GRCGYC	2 cut(s)	640, 1103
Hin1II	CATG	5 cut(s)	587, 878, 1792, 1923, 2071
Hin6I	GCGC	1 cut(s)	1442
HinP1I	GCGC	1 cut(s)	1442
HincII	GTYRAC	3 cut(s)	133, 858, 1138
HindII	GTYRAC	3 cut(s)	133, 858, 1138
HindIII	AAGCTT	1 cut(s)	428
HinfI	GANTC	6 cut(s)	32, 253, 1321, 1360, 1467, 2139
HpaII	CCGG	2 cut(s)	220, 330
HphI	GGTGA	2 cut(s)	1577, 1982
Hpy166II	GTNNAC	8 cut(s)	133, 363, 858, 1047, 1138, 1568, 1720, 1777
Hpy188I	TCNGA	8 cut(s)	700, 835, 1312, 1426, 1582, 1823, 1836, 2012
Hpy8I	GTNNAC	8 cut(s)	133, 363, 858, 1047, 1138, 1568, 1720, 1777
Hpy99I	CGWCG	6 cut(s)	134, 137, 140, 642, 729, 1949
HpyAV	CCTTC	5 cut(s)	180, 1428, 1645, 1882, 2103
HpyCH4III	ACNGT	5 cut(s)	27, 862, 1142, 1755, 1876
HpyCH4IV	ACGT	3 cut(s)	1024, 1103, 1701
HpyCH4V	TGCA	8 cut(s)	363, 401, 770, 1298, 1484, 1544, 1919, 1934
HpyF3I	CTNAG	8 cut(s)	312, 521, 648, 1012, 1029, 1518, 1820, 1833
HpySE526I	ACGT	3 cut(s)	1024, 1103, 1701
Hsp92I	GRCGYC	2 cut(s)	640, 1103
Hsp92II	CATG	5 cut(s)	587, 878, 1792, 1923, 2071
HspAI	GCGC	1 cut(s)	1442
KpnI	GGTACC	1 cut(s)	226
KspI	CCGCGG	1 cut(s)	973
Kzo9I	GATC	7 cut(s)	267, 538, 630, 745, 870, 1643, 2089
LmnI	GCTCC	5 cut(s)	17, 763, 823, 1036, 2119
LweI	GCATC	7 cut(s)	922, 984, 1226, 1421, 1471, 1921, 1943
MabI	ACCWGGT	1 cut(s)	243
MaeI	CTAG	3 cut(s)	1224, 1472, 1899
MaeII	ACGT	3 cut(s)	1024, 1103, 1701
MaeIII	GTNAC	4 cut(s)	469, 484, 1268, 1940
MalI	GATC	7 cut(s)	269, 540, 632, 747, 872, 1645, 2091
MbiI	CCGCTC	2 cut(s)	193, 1006
MboI	GATC	7 cut(s)	267, 538, 630, 745, 870, 1643, 2089
MflI	RGATCY	3 cut(s)	630, 745, 2089
MhlI	GDGCHC	2 cut(s)	22, 365
MlsI	TGGCCA	1 cut(s)	1408
MluCI	AATT	9 cut(s)	163, 306, 678, 701, 807, 1512, 1590, 1675, 1806
MluNI	TGGCCA	1 cut(s)	1408
MlyI	GAGTC	4 cut(s)	26, 247, 1476, 2133
MmeI	TCCRAC	2 cut(s)	9, 658
Mox20I	TGGCCA	1 cut(s)	1408
Mph1103I	ATGCAT	2 cut(s)	1921, 1936
MroXI	GAANNNNTTC	1 cut(s)	705
MscI	TGGCCA	1 cut(s)	1408
MseI	TTAA	4 cut(s)	798, 1367, 2016, 2158
MslI	CAYNNNNRTG	2 cut(s)	86, 1756
Msp20I	TGGCCA	1 cut(s)	1408
MspA1I	CMGCKG	3 cut(s)	291, 946, 972
MspI	CCGG	2 cut(s)	220, 330
MspR9I	CCNGG	7 cut(s)	9, 108, 245, 389, 881, 1260, 1394
MvaI	CCWGG	7 cut(s)	9, 108, 245, 389, 881, 1260, 1394
MvnI	CGCG	3 cut(s)	182, 410, 972
NdeII	GATC	7 cut(s)	267, 538, 630, 745, 870, 1643, 2089
NlaIII	CATG	5 cut(s)	587, 878, 1792, 1923, 2071
NmeAIII	GCCGAG	2 cut(s)	87, 1714
NmuCI	GTSAC	1 cut(s)	1940
NsiI	ATGCAT	2 cut(s)	1921, 1936
NspI	RCATGY	2 cut(s)	1923, 2071
OliI	CACNNNNGTG	1 cut(s)	1756
PagI	TCATGA	1 cut(s)	583
PcsI	WCGNNNNNNNCGW	1 cut(s)	135
PdmI	GAANNNNTTC	1 cut(s)	705
PfeI	GAWTC	2 cut(s)	1321, 1360
PflMI	CCANNNNNTGG	2 cut(s)	14, 28
PleI	GAGTC	4 cut(s)	26, 247, 1475, 2133
PmaCI	CACGTG	2 cut(s)	1025, 1702
PmlI	CACGTG	2 cut(s)	1025, 1702
PpsI	GAGTC	4 cut(s)	26, 247, 1475, 2133
Ppu21I	YACGTR	2 cut(s)	1025, 1702
Psp6I	CCWGG	7 cut(s)	7, 106, 243, 387, 879, 1258, 1392
PspCI	CACGTG	2 cut(s)	1025, 1702
PspFI	CCCAGC	1 cut(s)	366
PspGI	CCWGG	7 cut(s)	7, 106, 243, 387, 879, 1258, 1392
PspPI	GGNCC	3 cut(s)	5, 111, 752
PstI	CTGCAG	1 cut(s)	772
PstNI	CAGNNNCTG	2 cut(s)	398, 1039
PsuI	RGATCY	3 cut(s)	630, 745, 2089
PvuII	CAGCTG	1 cut(s)	946
RsaI	GTAC	8 cut(s)	179, 224, 317, 804, 942, 1955, 2036, 2066
RsaNI	GTAC	8 cut(s)	178, 223, 316, 803, 941, 1954, 2035, 2065
RseI	CAYNNNNRTG	2 cut(s)	86, 1756
SacII	CCGCGG	1 cut(s)	973
SalI	GTCGAC	1 cut(s)	131
SaqAI	TTAA	4 cut(s)	798, 1367, 2016, 2158
Sau3AI	GATC	7 cut(s)	267, 538, 630, 745, 870, 1643, 2089
Sau96I	GGNCC	3 cut(s)	5, 111, 752
SchI	GAGTC	4 cut(s)	26, 247, 1476, 2133
ScrFI	CCNGG	7 cut(s)	9, 108, 245, 389, 881, 1260, 1394
SduI	GDGCHC	2 cut(s)	22, 365
SexAI	ACCWGGT	1 cut(s)	243
SfaNI	GCATC	7 cut(s)	922, 984, 1226, 1421, 1471, 1921, 1943
SfcI	CTRYAG	1 cut(s)	768
Sfr303I	CCGCGG	1 cut(s)	973
SgrBI	CCGCGG	1 cut(s)	973
SgrDI	CGTCGACG	1 cut(s)	131
SinI	GGWCC	2 cut(s)	5, 111
SmiMI	CAYNNNNRTG	2 cut(s)	86, 1756
SmlI	CTYRAG	1 cut(s)	1974
SmoI	CTYRAG	1 cut(s)	1974
SpeI	ACTAGT	1 cut(s)	1898
Sse9I	AATT	9 cut(s)	163, 306, 678, 701, 807, 1512, 1590, 1675, 1806
SspMI	CTAG	3 cut(s)	1224, 1472, 1899
StyD4I	CCNGG	7 cut(s)	7, 106, 243, 387, 879, 1258, 1392
StyI	CCWWGG	3 cut(s)	371, 1212, 1374
TaaI	ACNGT	5 cut(s)	27, 862, 1142, 1755, 1876
TaiI	ACGT	3 cut(s)	1027, 1106, 1704
TaqI	TCGA	7 cut(s)	132, 692, 1151, 1438, 1924, 2084, 2147
TaqII	GACCGA	1 cut(s)	128
TasI	AATT	9 cut(s)	163, 306, 678, 701, 807, 1512, 1590, 1675, 1806
TatI	WGTACW	4 cut(s)	802, 940, 1953, 2064
TauI	GCSGC	4 cut(s)	196, 294, 1006, 1685
TfiI	GAWTC	2 cut(s)	1321, 1360
Tru1I	TTAA	4 cut(s)	798, 1367, 2016, 2158
Tru9I	TTAA	4 cut(s)	798, 1367, 2016, 2158
TscAI	CASTG	4 cut(s)	235, 1147, 1760, 1881
TseFI	GTSAC	1 cut(s)	1940
Tsp45I	GTSAC	1 cut(s)	1940
TspDTI	ATGAA	4 cut(s)	600, 800, 1677, 1805
TspGWI	ACGGA	5 cut(s)	64, 357, 753, 1298, 1990
TspRI	CASTG	4 cut(s)	235, 1147, 1760, 1881
Van91I	CCANNNNNTGG	2 cut(s)	14, 28
VneI	GTGCAC	1 cut(s)	361
VpaK11BI	GGWCC	2 cut(s)	5, 111
XapI	RAATTY	4 cut(s)	306, 701, 807, 1675
XceI	RCATGY	2 cut(s)	1923, 2071
XcmI	CCANNNNNNNNNTGG	1 cut(s)	241
XmiI	GTMKAC	2 cut(s)	132, 1719
XmnI	GAANNNNTTC	1 cut(s)	705
XspI	CTAG	3 cut(s)	1224, 1472, 1899
ZraI	GACGTC	1 cut(s)	1104
Zsp2I	ATGCAT	2 cut(s)	1921, 1936

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.