Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0005801

Physical Location & Seq

Genomic Coordinates Forward (+)

7730295 .. 7737388

Gene Structure 7094 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031717

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2082 bp

ATGTCACAGTTTTGGAAGCCAGGGGCGGAGAAGCCTCGCCTCCTCGACGACGAAGAAGGCGGCGTTGTTTTCTTCCCCACCTCCTCCGGAGGAGGCTATGCGAATCTTGAGAGGCAGAGGCAGAGACTACCGGTTTTCAGGTACCGCACCGCCATTCTCTACTCCGTCGAGACTCGCGCCACCACCGTCGTCGTCGGCGAAACCGGCAGCGGCAAAACCACTCAGATTCCTCAGTATTTGAAAGAAGCAGGTTGGGCTGATGGTGGCCGTGTAATTGCTTGTACTCAGCCGAGAAGATTGGCTGTCCAGTCGGTGGCGGCACGGGTGGCTGAAGAAATGGGGGTCAAGCTTGGAGAAGAAGTTGGCTACACTATCCGATTTGAAGATGCTACAAGTACAGGTGTGACTATGATAAAGTTTTTGACTGATGGTGTGTTACTCAGGGAAATGATGGACGATCCTCTTTTGACTAAATATAGTGTTGTAATGGTAGATGAAGCTCATGAAAGGTCTATCTCCACGGACATTTTATTGGGTCTCCTGAAAAAGATCCAAAAACGCCGGCCTGAGCTGCGCCTGATTATCTCATCTGCTACAATTCAAGCAAAGTCAATGGCTGCTTTCTTTCAGGCCAGCAGCAAAAGGCACCGCAGACCGGAAGGAGAAGAGCTTGGACCAATTATGGAGCCTGCAGTCCTGTCAGTTGAGGGTAGAGGGTTTAATGTACAAATTCATTATGTGGAGGAGCCTGTTGCCGACTATCTCCGGGCTGCAGTCTCAACGGTATTATTGATACATGACCAGGAACCAATGGGAGATATTCTGGTATTTCTTACTGGTCAAGATGATATCGATGCTGCTGTCCAACTTATTATGGAAGAGACCCAAAACCGCAGAAAGAACTCATCAGGACTGATGGTTTTGCCTCTCTATTCGGGACTTCCACGTGCTGACCAGGAGCTGGTGTTCAGTCCTACTCCTAGAGGGAAAAGGAAAGTAGTTATATCTACCAACATAGCAGAGACATCAATAACTTTAGAGGGCGTGGTCTATGTTGTTGACAGTGGGTTCTCAAAGCAACGGTTCTATAACCCGATATCAGATATTGAAAATCTGGTAGTGGCACCAATATCCAAGGCATCTGCTAGGCAAAGGACCGGTAGAGCTGGAAGAGTTCGGCCTGGGAAATGTTACAGGCTGTATACAGAAGAGTATTTTGTAAAGGAAATGTCTGCTGAGGGGATTCCAGAGATACAAAGATCAAATCTTGTTTCCTGTGTGATCCAGTTAAAAGCCTTGGGAATAGATAATATATTGGGCTTTGATTGGCCAGCATCTCCATCTTCTGAAGCAATGGTTCGAGCACTTGAAGTGCTTTATTCACTTGGGGTCCTGGATGATGATGCTAAACTTACTTCACCAACTGGATTTCAAGTTGCTGAGTTTCCATTGGATCCAATGATCTCAAAGATGATCTTATCATCTGGTGAACTGGGGTGTTCAGAAGAGATTATTACTATTGCGGCTGTTCTCTCTGTTCAATCTATCTGGGCTTCCGGTTGGGGATCACAAAAGGAACCTGATGAAGCCAAGTTGAATTTTGCTGCAGCTGAGGGTGACCATGTGAGTTTCCTAAATGTCTACAAAGCATTTCTTCAGTCTGGTAAATCTTCACAGTGGTGTCATAAGAACTTTGTAAACTACCAAGCCATGAAAAAGGTTCTTGAAATAAGGGAACAACTCAGAAGAACAGCTCGAAGATTAGGCATTGACTTGAAATCTTGTGAAAGAGATACAGTGGTAGTGAGAAAGGCCATTACTTATGGATTTTTCGCTAATGCATGTGTTTCAGAAGCATCAAGTCACGATGGAAAGTACAAAACTATCAGAGGTTCTCAAGAAGTTTATATTCACCCCTCATCTGTGCTATTCAGAGTTAATCCCAAGTGGGTGGTTTACCATTCTCTTGTCTCAACTGAAAAGCAATACATGCGGAACGTCATTTCGATAGATCCTTCTTGGCTCACAGAGGCAGCTCCACATTTTTACCAAAAGCAGCGACCCAATCCTATGCTTCACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030054 GO:0032991 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0055044 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_22 HA2 Helicase_C OB_NTP_bind

Protein Analysis

694

Amino Acids

77.23

Weight (kDa)

8.0

Isoelectric Point (pI)

46.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_22	PF13401	58 - 186	5.7e-07	AAA domain
DEAD	PF00270	58 - 204	2.9e-06	DEAD/DEAH box helicase
Helicase_C	PF00271	264 - 392	1.2e-12	Helicase conserved C-terminal domain
WHD_HA2	PF04408	455 - 484	5.8e-06	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	485 - 543	4.4e-10	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	602 - 681	6.3e-23	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005801)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18465
fragaria_vesca	FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450 FvH4_5g21450
malus_domestica	MD05G1305600.v1.1
prunus_persica	Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1 Prupe.4G056700_v2.0.a1
pyrus_communis	pycom05g28290
rosa_chinensis	RchiOBHm_Chr2g0158101 RchiOBHm_Chr5g0009991
rosa_laevigata	RLG00000031717
rosa_multiflora	Rmu_sc0001648.1_g000001 Rmu_sc0002275.1_g000005
rosa_roxburghii	Rroxscaffold_1G00066170
rosa_rugosa	Rorug04G0448300 Rorug04G0448400 Rorug04G0448500
rosa_samantha	Rh5AG079200 Rh5BG074000 Rh5CG086600 Rh5DG074700
rosa_wichuraiana	Rw5G007330 Rw7G028410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	239
Acc65I	GGTACC	1 cut(s)	141
AccB1I	GGYRCC	3 cut(s)	141, 645, 1123
AccB7I	CCANNNNNTGG	2 cut(s)	313, 961
AccI	GTMKAC	2 cut(s)	1202, 1641
AccII	CGCG	1 cut(s)	177
AccIII	TCCGGA	1 cut(s)	86
AclWI	GGATC	7 cut(s)	452, 544, 1276, 1448, 1461, 1573, 2006
AcoI	YGGCCR	2 cut(s)	265, 1328
AcsI	RAATTY	2 cut(s)	729, 1597
AcuI	CTGAAG	3 cut(s)	351, 1368, 1640
AcvI	CACGTG	1 cut(s)	947
AfaI	GTAC	5 cut(s)	143, 283, 397, 726, 1877
AfiI	CCNNNNNNNGG	3 cut(s)	313, 655, 961
AgeI	ACCGGT	2 cut(s)	130, 1157
AjnI	CCWGG	5 cut(s)	19, 801, 954, 1180, 1392
AleI	CACNNNNGTG	1 cut(s)	1678
AloI	GAACNNNNNNTCC	2 cut(s)	950, 982
AluBI	AGCT	9 cut(s)	349, 500, 571, 670, 961, 1166, 1610, 1754, 2036
AluI	AGCT	9 cut(s)	349, 500, 571, 670, 961, 1166, 1610, 1754, 2036
Alw21I	GWGCWC	1 cut(s)	1366
Alw26I	GTCTC	7 cut(s)	118, 164, 542, 781, 875, 1016, 1975
AlwI	GGATC	7 cut(s)	452, 544, 1276, 1448, 1461, 1573, 2006
AlwNI	CAGNNNCTG	1 cut(s)	961
Aor13HI	TCCGGA	1 cut(s)	86
AoxI	GGCC	6 cut(s)	265, 563, 630, 1178, 1328, 1812
ApoI	RAATTY	2 cut(s)	729, 1597
AsiGI	ACCGGT	2 cut(s)	130, 1157
Asp718I	GGTACC	1 cut(s)	141
AspLEI	GCGC	2 cut(s)	179, 576
AspS9I	GGNCC	3 cut(s)	674, 1155, 1390
AsuC2I	CCSGG	1 cut(s)	767
AsuHPI	GGTGA	4 cut(s)	1410, 1499, 1628, 1904
AvaII	GGWCC	3 cut(s)	674, 1155, 1390
BaeI	ACNNNNGTAYC	2 cut(s)	1785, 1818
BalI	TGGCCA	1 cut(s)	1330
BamHI	GGATCC	1 cut(s)	1453
BanI	GGYRCC	3 cut(s)	141, 645, 1123
BbrPI	CACGTG	1 cut(s)	947
Bbv12I	GWGCWC	1 cut(s)	1366
BbvCI	CCTCAGC	2 cut(s)	1236, 1611
BccI	CCATC	6 cut(s)	254, 422, 445, 910, 1348, 1862
BceAI	ACGGC	1 cut(s)	252
BciT130I	CCWGG	5 cut(s)	21, 803, 956, 1182, 1394
BcnI	CCSGG	1 cut(s)	767
BcoDI	GTCTC	7 cut(s)	118, 164, 542, 781, 875, 1016, 1975
BfaI	CTAG	3 cut(s)	981, 1146, 2080
BfmI	CTRYAG	3 cut(s)	690, 771, 1605
BfuAI	ACCTGC	1 cut(s)	239
Bme1390I	CCNGG	6 cut(s)	21, 767, 803, 956, 1182, 1394
Bme18I	GGWCC	3 cut(s)	674, 1155, 1390
BmgT120I	GGNCC	3 cut(s)	674, 1155, 1390
BmiI	GGNNCC	9 cut(s)	143, 647, 687, 747, 807, 1125, 1391, 1455, 1578
BmrFI	CCNGG	6 cut(s)	21, 767, 803, 956, 1182, 1394
BmrI	ACTGGG	1 cut(s)	1502
BmsI	GCATC	6 cut(s)	376, 844, 1148, 1343, 1393, 1865
BmuI	ACTGGG	1 cut(s)	1502
Bpu10I	CCTNAGC	3 cut(s)	567, 1236, 1611
BpuEI	CTTGAG	2 cut(s)	128, 1881
BpuMI	CCSGG	1 cut(s)	767
Bsa29I	ATCGAT	1 cut(s)	852
BsaAI	YACGTR	1 cut(s)	947
BsaI	GGTCTC	2 cut(s)	542, 875
BsaJI	CCNNGG	5 cut(s)	20, 519, 1134, 1181, 1296
BsaWI	WCCGGW	5 cut(s)	86, 130, 655, 1157, 1556
BsaXI	ACNNNNNCTCC	4 cut(s)	345, 375, 950, 980
Bsc4I	CCNNNNNNNGG	3 cut(s)	313, 655, 961
Bse118I	RCCGGY	4 cut(s)	130, 203, 561, 1157
Bse1I	ACTGG	5 cut(s)	307, 841, 1285, 1429, 1497
Bse3DI	GCAATG	1 cut(s)	1359
BseAI	TCCGGA	1 cut(s)	86
BseBI	CCWGG	5 cut(s)	21, 803, 956, 1182, 1394
BseCI	ATCGAT	1 cut(s)	852
BseDI	CCNNGG	5 cut(s)	20, 519, 1134, 1181, 1296
BseGI	GGATG	1 cut(s)	1402
BseLI	CCNNNNNNNGG	3 cut(s)	313, 655, 961
BseMI	GCAATG	1 cut(s)	1359
BseMII	CTCAG	9 cut(s)	236, 245, 299, 454, 558, 1227, 1431, 1602, 1756
BseNI	ACTGG	5 cut(s)	307, 841, 1285, 1429, 1497
BseRI	GAGGAG	4 cut(s)	32, 73, 105, 758
Bsh1236I	CGCG	1 cut(s)	177
BshFI	GGCC	6 cut(s)	267, 565, 632, 1180, 1330, 1814
BshNI	GGYRCC	3 cut(s)	141, 645, 1123
BshTI	ACCGGT	2 cut(s)	130, 1157
BshVI	ATCGAT	1 cut(s)	852
BsiHKAI	GWGCWC	1 cut(s)	1366
BsiSI	CCGG	8 cut(s)	87, 131, 204, 562, 656, 766, 1158, 1557
BslFI	GGGAC	1 cut(s)	951
BslI	CCNNNNNNNGG	3 cut(s)	313, 655, 961
BsmAI	GTCTC	7 cut(s)	118, 164, 542, 781, 875, 1016, 1975
BsmFI	GGGAC	1 cut(s)	951
BsnI	GGCC	6 cut(s)	267, 565, 632, 1180, 1330, 1814
Bso31I	GGTCTC	2 cut(s)	542, 875
Bsp1286I	GDGCHC	1 cut(s)	1366
Bsp13I	TCCGGA	1 cut(s)	86
Bsp1407I	TGTACA	1 cut(s)	724
Bsp143I	GATC	9 cut(s)	457, 549, 1259, 1281, 1453, 1461, 1473, 1565, 2011
BspANI	GGCC	6 cut(s)	267, 565, 632, 1180, 1330, 1814
BspCNI	CTCAG	9 cut(s)	235, 244, 298, 453, 559, 1228, 1432, 1603, 1755
BspDI	ATCGAT	1 cut(s)	852
BspEI	TCCGGA	1 cut(s)	86
BspFNI	CGCG	1 cut(s)	177
BspHI	TCATGA	1 cut(s)	502
BspLI	GGNNCC	9 cut(s)	143, 647, 687, 747, 807, 1125, 1391, 1455, 1578
BspMAI	CTGCAG	3 cut(s)	694, 775, 1609
BspMI	ACCTGC	1 cut(s)	239
BspPI	GGATC	7 cut(s)	452, 544, 1276, 1448, 1461, 1573, 2006
BspQI	GCTCTTC	1 cut(s)	660
BspT107I	GGYRCC	3 cut(s)	141, 645, 1123
BspTNI	GGTCTC	2 cut(s)	542, 875
BsrDI	GCAATG	1 cut(s)	1359
BsrFI	RCCGGY	4 cut(s)	130, 203, 561, 1157
BsrGI	TGTACA	1 cut(s)	724
BsrI	ACTGG	5 cut(s)	307, 841, 1285, 1429, 1497
BssAI	RCCGGY	4 cut(s)	130, 203, 561, 1157
BssECI	CCNNGG	5 cut(s)	20, 519, 1134, 1181, 1296
BssMI	GATC	9 cut(s)	457, 549, 1259, 1281, 1453, 1461, 1473, 1565, 2011
BssNAI	GTATAC	1 cut(s)	1203
BssT1I	CCWWGG	2 cut(s)	1134, 1296
Bst1107I	GTATAC	1 cut(s)	1203
Bst2UI	CCWGG	5 cut(s)	21, 803, 956, 1182, 1394
Bst4CI	ACNGT	7 cut(s)	9, 187, 784, 1064, 1083, 1677, 1798
Bst6I	CTCTTC	5 cut(s)	660, 873, 1165, 1203, 1500
BstAPI	GCANNNNNTGC	1 cut(s)	1990
BstAUI	TGTACA	1 cut(s)	724
BstBAI	YACGTR	1 cut(s)	947
BstC8I	GCNNGC	4 cut(s)	563, 634, 690, 1332
BstDEI	CTNAG	9 cut(s)	222, 231, 285, 440, 567, 1236, 1440, 1611, 1742
BstDSI	CCRYGG	1 cut(s)	519
BstEII	GGTNACC	1 cut(s)	1616
BstF5I	GGATG	1 cut(s)	1402
BstFNI	CGCG	1 cut(s)	177
BstHHI	GCGC	2 cut(s)	179, 576
BstKTI	GATC	9 cut(s)	460, 552, 1262, 1284, 1456, 1464, 1476, 1568, 2014
BstMAI	GTCTC	7 cut(s)	118, 164, 542, 781, 875, 1016, 1975
BstMBI	GATC	9 cut(s)	457, 549, 1259, 1281, 1453, 1461, 1473, 1565, 2011
BstMWI	GCNNNNNNNGC	5 cut(s)	204, 254, 326, 571, 1990
BstNI	CCWGG	5 cut(s)	21, 803, 956, 1182, 1394
BstNSI	RCATGY	2 cut(s)	1845, 1993
BstPI	GGTNACC	1 cut(s)	1616
BstSCI	CCNGG	6 cut(s)	19, 765, 801, 954, 1180, 1392
BstSFI	CTRYAG	3 cut(s)	690, 771, 1605
BstUI	CGCG	1 cut(s)	177
BstX2I	RGATCY	3 cut(s)	549, 1453, 2011
BstXI	CCANNNNNNTGG	1 cut(s)	1951
BstYI	RGATCY	3 cut(s)	549, 1453, 2011
BstZ17I	GTATAC	1 cut(s)	1203
Bsu15I	ATCGAT	1 cut(s)	852
BsuRI	GGCC	6 cut(s)	267, 565, 632, 1180, 1330, 1814
BsuTUI	ATCGAT	1 cut(s)	852
BtgI	CCRYGG	1 cut(s)	519
BtsCI	GGATG	1 cut(s)	1402
BtsIMutI	CAGTG	3 cut(s)	1069, 1682, 1803
BveI	ACCTGC	1 cut(s)	239
Cac8I	GCNNGC	4 cut(s)	563, 634, 690, 1332
CaiI	CAGNNNCTG	1 cut(s)	961
CciI	TCATGA	1 cut(s)	502
CfoI	GCGC	2 cut(s)	179, 576
Cfr10I	RCCGGY	4 cut(s)	130, 203, 561, 1157
Cfr13I	GGNCC	3 cut(s)	674, 1155, 1390
ClaI	ATCGAT	1 cut(s)	852
Csp6I	GTAC	5 cut(s)	142, 282, 396, 725, 1876
CspAI	ACCGGT	2 cut(s)	130, 1157
CviAII	CATG	6 cut(s)	503, 797, 1622, 1711, 1842, 1990
CviQI	GTAC	5 cut(s)	142, 282, 396, 725, 1876
DdeI	CTNAG	9 cut(s)	222, 231, 285, 440, 567, 1236, 1440, 1611, 1742
DpnI	GATC	9 cut(s)	459, 551, 1261, 1283, 1455, 1463, 1475, 1567, 2013
DpnII	GATC	9 cut(s)	457, 549, 1259, 1281, 1453, 1461, 1473, 1565, 2011
EaeI	YGGCCR	2 cut(s)	265, 1328
Eam1104I	CTCTTC	5 cut(s)	660, 873, 1165, 1203, 1500
EarI	CTCTTC	5 cut(s)	660, 873, 1165, 1203, 1500
EciI	GGCGGA	1 cut(s)	41
Eco130I	CCWWGG	2 cut(s)	1134, 1296
Eco31I	GGTCTC	2 cut(s)	542, 875
Eco32I	GATATC	2 cut(s)	850, 1098
Eco47I	GGWCC	3 cut(s)	674, 1155, 1390
Eco57I	CTGAAG	3 cut(s)	351, 1368, 1640
Eco72I	CACGTG	1 cut(s)	947
Eco91I	GGTNACC	1 cut(s)	1616
EcoO109I	RGGNCCY	1 cut(s)	1390
EcoO65I	GGTNACC	1 cut(s)	1616
EcoRII	CCWGG	5 cut(s)	19, 801, 954, 1180, 1392
EcoRV	GATATC	2 cut(s)	850, 1098
EcoT14I	CCWWGG	2 cut(s)	1134, 1296
EcoT22I	ATGCAT	1 cut(s)	1843
ErhI	CCWWGG	2 cut(s)	1134, 1296
FaeI	CATG	6 cut(s)	506, 800, 1625, 1714, 1845, 1993
FalI	AAGNNNNNCTT	4 cut(s)	1460, 1492, 1638, 1670
FaqI	GGGAC	1 cut(s)	951
FatI	CATG	6 cut(s)	502, 796, 1621, 1710, 1841, 1989
FblI	GTMKAC	2 cut(s)	1202, 1641
FokI	GGATG	1 cut(s)	1409
FspBI	CTAG	3 cut(s)	981, 1146, 2080
GlaI	GCGC	2 cut(s)	178, 575
HaeIII	GGCC	6 cut(s)	267, 565, 632, 1180, 1330, 1814
HapII	CCGG	8 cut(s)	87, 131, 204, 562, 656, 766, 1158, 1557
HhaI	GCGC	2 cut(s)	179, 576
Hin1II	CATG	6 cut(s)	506, 800, 1625, 1714, 1845, 1993
Hin6I	GCGC	2 cut(s)	177, 574
HinP1I	GCGC	2 cut(s)	177, 574
HincII	GTYRAC	1 cut(s)	1060
HindII	GTYRAC	1 cut(s)	1060
HindIII	AAGCTT	1 cut(s)	347
HinfI	GANTC	4 cut(s)	103, 172, 226, 1243
HpaII	CCGG	8 cut(s)	87, 131, 204, 562, 656, 766, 1158, 1557
HphI	GGTGA	4 cut(s)	1410, 1499, 1628, 1904
Hpy166II	GTNNAC	6 cut(s)	1060, 1203, 1490, 1642, 1699, 1957
Hpy188I	TCNGA	9 cut(s)	225, 377, 1102, 1348, 1504, 1745, 1852, 1889, 1934
Hpy8I	GTNNAC	6 cut(s)	1060, 1203, 1490, 1642, 1699, 1957
Hpy99I	CGWCG	6 cut(s)	50, 53, 170, 191, 194, 197
HpyAV	CCTTC	3 cut(s)	50, 653, 2025
HpyCH4III	ACNGT	7 cut(s)	9, 187, 784, 1064, 1083, 1677, 1798
HpyCH4IV	ACGT	2 cut(s)	946, 1998
HpyCH4V	TGCA	4 cut(s)	692, 773, 1607, 1841
HpyF10VI	GCNNNNNNNGC	5 cut(s)	204, 254, 326, 571, 1990
HpyF3I	CTNAG	9 cut(s)	222, 231, 285, 440, 567, 1236, 1440, 1611, 1742
HpySE526I	ACGT	2 cut(s)	946, 1998
Hsp92II	CATG	6 cut(s)	506, 800, 1625, 1714, 1845, 1993
HspAI	GCGC	2 cut(s)	177, 574
Kpn2I	TCCGGA	1 cut(s)	86
KpnI	GGTACC	1 cut(s)	145
KroI	GCCGGC	1 cut(s)	561
KroNI	GCCGGC	1 cut(s)	563
Kzo9I	GATC	9 cut(s)	457, 549, 1259, 1281, 1453, 1461, 1473, 1565, 2011
LguI	GCTCTTC	1 cut(s)	660
LmnI	GCTCC	4 cut(s)	685, 745, 958, 2041
LweI	GCATC	6 cut(s)	376, 844, 1148, 1343, 1393, 1865
MaeI	CTAG	3 cut(s)	981, 1146, 2080
MaeII	ACGT	2 cut(s)	946, 1998
MaeIII	GTNAC	6 cut(s)	3, 403, 435, 1190, 1616, 1862
MalI	GATC	9 cut(s)	459, 551, 1261, 1283, 1455, 1463, 1475, 1567, 2013
MboI	GATC	9 cut(s)	457, 549, 1259, 1281, 1453, 1461, 1473, 1565, 2011
MflI	RGATCY	3 cut(s)	549, 1453, 2011
MhlI	GDGCHC	1 cut(s)	1366
MlsI	TGGCCA	1 cut(s)	1330
MluCI	AATT	5 cut(s)	273, 597, 678, 729, 1597
MluNI	TGGCCA	1 cut(s)	1330
MlyI	GAGTC	1 cut(s)	166
MmeI	TCCRAC	1 cut(s)	889
Mox20I	TGGCCA	1 cut(s)	1330
Mph1103I	ATGCAT	1 cut(s)	1843
MroI	TCCGGA	1 cut(s)	86
MroNI	GCCGGC	1 cut(s)	561
MscI	TGGCCA	1 cut(s)	1330
MseI	TTAA	3 cut(s)	720, 1289, 1938
MslI	CAYNNNNRTG	1 cut(s)	1678
Msp20I	TGGCCA	1 cut(s)	1330
MspA1I	CMGCKG	2 cut(s)	210, 1610
MspI	CCGG	8 cut(s)	87, 131, 204, 562, 656, 766, 1158, 1557
MspR9I	CCNGG	6 cut(s)	21, 767, 803, 956, 1182, 1394
MvaI	CCWGG	5 cut(s)	21, 803, 956, 1182, 1394
MvnI	CGCG	1 cut(s)	177
MwoI	GCNNNNNNNGC	5 cut(s)	204, 254, 326, 571, 1990
NaeI	GCCGGC	1 cut(s)	563
NciI	CCSGG	1 cut(s)	767
NdeII	GATC	9 cut(s)	457, 549, 1259, 1281, 1453, 1461, 1473, 1565, 2011
NgoMIV	GCCGGC	1 cut(s)	561
NlaIII	CATG	6 cut(s)	506, 800, 1625, 1714, 1845, 1993
NlaIV	GGNNCC	9 cut(s)	143, 647, 687, 747, 807, 1125, 1391, 1455, 1578
NmeAIII	GCCGAG	1 cut(s)	315
NmuCI	GTSAC	4 cut(s)	3, 403, 1616, 1862
NsiI	ATGCAT	1 cut(s)	1843
NspI	RCATGY	2 cut(s)	1845, 1993
OliI	CACNNNNGTG	1 cut(s)	1678
PagI	TCATGA	1 cut(s)	502
PciSI	GCTCTTC	1 cut(s)	660
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PdiI	GCCGGC	1 cut(s)	563
PfeI	GAWTC	3 cut(s)	103, 226, 1243
PflMI	CCANNNNNTGG	2 cut(s)	313, 961
PfoI	TCCNGGA	1 cut(s)	1392
PinAI	ACCGGT	2 cut(s)	130, 1157
PleI	GAGTC	1 cut(s)	166
PmaCI	CACGTG	1 cut(s)	947
PmlI	CACGTG	1 cut(s)	947
PpsI	GAGTC	1 cut(s)	166
Ppu21I	YACGTR	1 cut(s)	947
PpuMI	RGGWCCY	1 cut(s)	1390
Psp5II	RGGWCCY	1 cut(s)	1390
Psp6I	CCWGG	5 cut(s)	19, 801, 954, 1180, 1392
PspCI	CACGTG	1 cut(s)	947
PspEI	GGTNACC	1 cut(s)	1616
PspGI	CCWGG	5 cut(s)	19, 801, 954, 1180, 1392
PspN4I	GGNNCC	9 cut(s)	143, 647, 687, 747, 807, 1125, 1391, 1455, 1578
PspPI	GGNCC	3 cut(s)	674, 1155, 1390
PspPPI	RGGWCCY	1 cut(s)	1390
PstI	CTGCAG	3 cut(s)	694, 775, 1609
PstNI	CAGNNNCTG	1 cut(s)	961
PsuI	RGATCY	3 cut(s)	549, 1453, 2011
PvuII	CAGCTG	1 cut(s)	1610
RsaI	GTAC	5 cut(s)	143, 283, 397, 726, 1877
RsaNI	GTAC	5 cut(s)	142, 282, 396, 725, 1876
RseI	CAYNNNNRTG	1 cut(s)	1678
SapI	GCTCTTC	1 cut(s)	660
SaqAI	TTAA	3 cut(s)	720, 1289, 1938
Sau3AI	GATC	9 cut(s)	457, 549, 1259, 1281, 1453, 1461, 1473, 1565, 2011
Sau96I	GGNCC	3 cut(s)	674, 1155, 1390
SchI	GAGTC	1 cut(s)	166
ScrFI	CCNGG	6 cut(s)	21, 767, 803, 956, 1182, 1394
SduI	GDGCHC	1 cut(s)	1366
SfaNI	GCATC	6 cut(s)	376, 844, 1148, 1343, 1393, 1865
SfcI	CTRYAG	3 cut(s)	690, 771, 1605
SinI	GGWCC	3 cut(s)	674, 1155, 1390
SmiMI	CAYNNNNRTG	1 cut(s)	1678
SmlI	CTYRAG	2 cut(s)	107, 1896
SmoI	CTYRAG	2 cut(s)	107, 1896
Sse9I	AATT	5 cut(s)	273, 597, 678, 729, 1597
SspMI	CTAG	3 cut(s)	981, 1146, 2080
StyD4I	CCNGG	6 cut(s)	19, 765, 801, 954, 1180, 1392
StyI	CCWWGG	2 cut(s)	1134, 1296
TaaI	ACNGT	7 cut(s)	9, 187, 784, 1064, 1083, 1677, 1798
TaiI	ACGT	2 cut(s)	949, 2001
TaqI	TCGA	6 cut(s)	45, 168, 852, 1360, 1756, 2006
TasI	AATT	5 cut(s)	273, 597, 678, 729, 1597
TatI	WGTACW	4 cut(s)	281, 395, 724, 1875
TauI	GCSGC	4 cut(s)	63, 213, 320, 1526
TfiI	GAWTC	3 cut(s)	103, 226, 1243
Tru1I	TTAA	3 cut(s)	720, 1289, 1938
Tru9I	TTAA	3 cut(s)	720, 1289, 1938
TscAI	CASTG	3 cut(s)	1069, 1682, 1803
TseFI	GTSAC	4 cut(s)	3, 403, 1616, 1862
Tsp45I	GTSAC	4 cut(s)	3, 403, 1616, 1862
TspDTI	ATGAA	5 cut(s)	510, 519, 722, 1599, 1727
TspGWI	ACGGA	2 cut(s)	154, 536
TspRI	CASTG	3 cut(s)	1069, 1682, 1803
Van91I	CCANNNNNTGG	2 cut(s)	313, 961
VpaK11BI	GGWCC	3 cut(s)	674, 1155, 1390
XapI	RAATTY	2 cut(s)	729, 1597
XceI	RCATGY	2 cut(s)	1845, 1993
XmiI	GTMKAC	2 cut(s)	1202, 1641
XspI	CTAG	3 cut(s)	981, 1146, 2080
Zsp2I	ATGCAT	1 cut(s)	1843

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.