Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005460

Physical Location & Seq

Genomic Coordinates Forward (+)

10615696 .. 10623524

Gene Structure 7829 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G19490.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3105 bp

ATGGATTCACACCCTTCCCTAATGGGAAGGTCCATTACCAATTCGAACCGATCGAGCTTAGATCTTGGTCGTCCTTCTTCGTCTTCTTCATCTTCTCCTTCACCGTTAACCAAATCGATCTCCGATGCAAGTAGTCAAAGTCTCTCTTCGATCCTTAACAATCCACACGGAGGTAAGTCTGGTGTTTATGGTTCCGATGCATCTTGGGTTGGTTGGTGGTCTTCATCGACATTTGTAGCTCCAGCTGAGTTTGCGCCTGTTGCTTCAACGAAGCTCCCTGGTTCGGAGCTAACCAGATCCGACTTCCATGGCTACGTTTCTTCGATCTCTGAGTCTCATGGTAGATTTGAGGATATTAGGAAGCATACTAGGGAAGAGAGCTGTGGGTTTGATCAGGAGAGCCATGTATCGGGTTTAGCTGCGTGTTTGCGTGAGGTTCCTTCGCTTTACTTTAAGGAGGATTTTGCTCTTGAGGACGGAGCTACATTTCGTTCTGCTTGCCCTTTCTCGAGCCTTAATGAGAATCTAGCGCTTCAAGAGAAGTTGTCACAGTATCTTGATGTGGTGGAGCTGCATCTTGTGAAGGAGATCTCTGTGAGGTCTGATTCGTTTTTTGAGGCTCAAGGACAGTTACAGGATTTGAATGTGAAGATTGTTGAGGGTTGTAGTAGGATTCGTGAGTTGAAAGAGACTATTCGGCTCCTGGATAGGAATTTGGTAGATTCAGCTCGACAGATTCAAGAGCTGAGCTCAACTAGGATTAATATGCTGGAGCTGCAACGTAAATTAAGACTTATTTTGTATGTTAACCAAGCTCTCTCTGCACTCAAATTGCTTGTTGCGTCCGCAGATTGTGCTGGAGCTTTGGATATAACTGACGATCTCCAGAACTTGCTGGCTGGGGATGAGCTTACTGGTCTATACTGCTTCCGCCATCTTCGGGATCATGTTACTAGTTCCATAGATTCCATAAACAGTATTCTCACTTCAGAATTCATGCGTATCTCCATACATGATACTGGGGAGATAGATGTACTGATTTTATCTGCAGCAAATATACGGGGATCCATTTCATCTAACGGGAATACCGGCGAAGAAGTTAAGTTAGAAGAAGAGGATACCTCTACCTTATGTGATCGGCTTCTTCCTTTAGTTATTGGATTGCTAAGAACGGCAAAGTTCCCGTCAATTTTGAGGATGTATCGTGATACACTCACATCCGAAATGAAAAATGCAATCAAGAAAGCAGTTGCTGATCTGCTTCCCATTCTTGTTGCAAGATCGCTCGAATCTGATTTTTCACATGGGGAGCGCTCTGTAGATGATGGTGGTGGCTTATCTCTCGCGAGCAAATTGAGGACTCTGTCATCTGAGGCTTTTGTTAATCTCTTAACTGCTATATTTAAGATTGTTCAGGCACATCTAGTCCGGGCTTCGGAAGTGAAAAAAGCAATTGAATGGATTCTCTGCAATATCGATGGACATTATGCCGCTGATTCAGTTGCAGCTGCAATTGCTGTTGGTGCTGTAGCAGCCGAAACAGCTCAAGAAATAGGTTTCCAAGGCGGGTCATTAGTGTCTTCTCCATTGGGTAAGGCTACCTCAAAGGCCCCTCCATTGCAGGGAAAATCGAGTGATGCATCAAGCTTGATGAACATGTCAAGAAACTTCAGGGCCGATGTCTTGAGAGAAAATACAGAAGCTGTCTTTGCGGCTTGTGAGGTTACTCATGGTAGGTGGGCAAAGCTACTAGGAGTTCGCGCTCTTCTTCATCCAAAATTGAAATTGCAAGAGTTCATGAGCATTTATGATCTGACCCAAGAATTTATAACATCTACGGAGAAGATTGGTGGAAGGTTGGGTTCTAGTATTCGTGGGACGCTGCAATCACAAGCCAAAGCCTTTGTGGACTCCCAGCACGAATCTCGGATGACAAAACTAAAAGCTGTGCTTGATCAAGAAACATGGGATGAGATAGATGTTCCTGAAGAATTTCAATCCATAATCAGTTCTCTTTTTGCATCCCAGCGGTTGATCTCTGGGAAAGTTGATGATGCTGATCTTAATTCATACCACAGTAATCGGCTCCCTCTCAATGGGTCTCTCACTTCTGGATCTGGGGATCAAAATAGTGAATTACGAAATGAAAAATCTGAATCTAGTGAAGGTTCTGTAGTTAGTGATGCACAAGTGAAACCCACAGTCTCACCTGAGTCTCTTGAAAGAAGCAAGGCTGGTGTATCTTCAGCTACGAATAATCAAAGTAATCAGAAGGCGCACGGAAAATCTAACCTTTTCTATCAAGGAGTTGGTTACCACATGGTAAACTGTGGGTTAATATTGCTCAAGATGCTGTCGGAATACATTGATATGAACAATTCTTTGCCAGCTTTGTCCTCGGAAATTGTTCTTCGTGTTGTTGAAGTGTTGAGATTTTTCAACACTCGGACATGCCAACTTGTTCTGGGTGCTGGTGCTATGCAGGTATCTGGTTTGAAGTCCATAAAGGCGAAACACTTGGCGCTTGCAAGTCAAGTAATCGACTTCACTTACACTATTATTCCTGAAACCAGAAGGATTCTGTTTTCAAAAGTACCTGAGACGAGAAAGCCTCTGTTGTCAGTAGAGATTGATAAAGTTGCTCAGGATTTTAGAATTCATCGAGATGAAATATACACAAAACTGGTTCAGATTATGAGAGAAAGACTATTGGCGCATCTTCATGGTTTGCCCAAAGTTGTTGAGGGCTGGAACAGACCTCCAGATACCAATAAACAAACTAAAGAGTTTGCATGGCCGCTCACAAGGGAAGTTGGCTACCTTCACCGTGTGTTGTCTGAGACATTGCACGAAGCCGATGTTCAAGCAATCTTCAGGCAGGTGATTTCAATTATCCACACACAAACCTCTCAAACGCTTACGAACTTGGAGATAAGCTCAACAGAGGCAAAGAAAAGGCTGAAGCTTCATGTCGAGCTAATCCTCAAATGCATCCGGTCGTTACCATCCGACAATGCCAATCAATCAGACATCCCAAACTGGGGACAACTAGATGAATTCTTTGCAGAGCACTTTAGAGAAGAAGAAGCAGGCGAAGCTGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000149 GO:0000938 GO:0001894 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005802 GO:0006810 GO:0006892 GO:0006896 GO:0006996 GO:0007010 GO:0007034 GO:0007041 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016482 GO:0019905 GO:0031090 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032501 GO:0032588 GO:0032991 GO:0040008 GO:0042147 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045103 GO:0045104 GO:0046907 GO:0048193 GO:0048471 GO:0048871 GO:0048872 GO:0048873 GO:0050789 GO:0050879 GO:0050881 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060052 GO:0060249 GO:0065007 GO:0065008 GO:0070013 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps54

Protein Analysis

1034

Amino Acids

113.86

Weight (kDa)

5.84

Isoelectric Point (pI)

45.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps54	PF07928	772 - 902	2.8e-43	Vps54-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0005460)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19490 AT4G19490
fragaria_vesca	FvH4_6g01010 FvH4_6g01010 FvH4_6g01010
malus_domestica	MD04G1237800.v1.1 MD12G1256000.v1.1
prunus_persica	Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1
pyrus_communis	pycom04g21100 pycom12g23480
rosa_chinensis	RchiOBHm_Chr3g0448451
rosa_laevigata	RLG00000000687 RLG00000025905
rosa_multiflora	Rmu_sc0003308.1_g000012 Rmu_sc0011979.1_g000005
rosa_roxburghii	Rroxscaffold_6G00426680
rosa_rugosa	Rorug02G0612500 Rorug02G0612600 Rorug02G0612600 Rorug07G0334800
rosa_samantha	Rh3AG012000 Rh3BG011800 Rh3CG011500 Rh3DG012200 Rh7BG461900
rosa_wichuraiana	Rw3G000930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1829
AarI	CACCTGC	1 cut(s)	2869
Acc36I	ACCTGC	2 cut(s)	2473, 2869
AccBSI	CCGCTC	1 cut(s)	2800
AccII	CGCG	2 cut(s)	1346, 1761
AciI	CCGC	7 cut(s)	846, 931, 1491, 1566, 1712, 2029, 2798
AclWI	GGATC	7 cut(s)	145, 291, 951, 1059, 1072, 2122, 2130
AcoI	YGGCCR	1 cut(s)	2795
AcsI	RAATTY	6 cut(s)	712, 992, 1823, 1991, 2655, 3056
AcuI	CTGAAG	6 cut(s)	972, 1654, 2007, 2229, 2857, 2981
AdeI	CACNNNGTG	1 cut(s)	2830
AfaI	GTAC	2 cut(s)	1035, 2595
AfeI	AGCGCT	2 cut(s)	531, 1313
AfiI	CCNNNNNNNGG	9 cut(s)	170, 283, 409, 940, 1435, 1622, 2096, 3040, 3041
AflIII	ACRYGT	1 cut(s)	1656
AhlI	ACTAGT	1 cut(s)	953
AjnI	CCWGG	2 cut(s)	277, 702
Alw21I	GWGCWC	2 cut(s)	752, 3072
Alw26I	GTCTC	8 cut(s)	146, 339, 683, 2106, 2209, 2220, 2594, 2834
AlwI	GGATC	7 cut(s)	145, 291, 951, 1059, 1072, 2122, 2130
AlwNI	CAGNNNCTG	2 cut(s)	1253, 1703
Ama87I	CYCGRG	1 cut(s)	508
Aor51HI	AGCGCT	2 cut(s)	531, 1313
AoxI	GGCC	3 cut(s)	1608, 1674, 2795
ApeKI	GCWGC	8 cut(s)	419, 571, 775, 1049, 1505, 1508, 1532, 1882
ApoI	RAATTY	6 cut(s)	712, 992, 1823, 1991, 2655, 3056
ArsI	GACNNNNNNTTYG	2 cut(s)	1169, 1201
AseI	ATTAAT	1 cut(s)	762
Asp700I	GAANNNNTTC	3 cut(s)	1461, 1992, 2406
AspLEI	GCGC	7 cut(s)	256, 532, 1314, 1763, 2278, 2524, 2716
AspS9I	GGNCC	3 cut(s)	30, 1609, 1674
AsuC2I	CCSGG	1 cut(s)	1430
AsuHPI	GGTGA	4 cut(s)	93, 2199, 2816, 2893
AsuII	TTCGAA	1 cut(s)	44
AvaI	CYCGRG	1 cut(s)	508
AvaII	GGWCC	1 cut(s)	30
BamHI	GGATCC	1 cut(s)	1064
BanII	GRGCYC	1 cut(s)	752
BarI	GAAGNNNNNNTAC	4 cut(s)	19, 51, 970, 1002
BbsI	GAAGAC	3 cut(s)	75, 213, 1572
Bbv12I	GWGCWC	2 cut(s)	752, 3072
BbvI	GCAGC	8 cut(s)	406, 558, 762, 1061, 1495, 1517, 1544, 1869
BccI	CCATC	4 cut(s)	942, 1319, 1472, 3013
BceAI	ACGGC	1 cut(s)	1188
BcgI	CGANNNNNNTGC	2 cut(s)	1907, 1941
BciT130I	CCWGG	2 cut(s)	279, 704
BciVI	GTATCC	1 cut(s)	1111
BclI	TGATCA	2 cut(s)	391, 1954
BcnI	CCSGG	1 cut(s)	1430
BcoDI	GTCTC	8 cut(s)	146, 339, 683, 2106, 2209, 2220, 2594, 2834
BcuI	ACTAGT	1 cut(s)	953
BfaI	CTAG	9 cut(s)	369, 527, 756, 954, 1424, 1751, 1866, 2160, 3050
BfmI	CTRYAG	4 cut(s)	1047, 1317, 1527, 2172
BfoI	RGCGCY	3 cut(s)	533, 1315, 2525
BfuAI	ACCTGC	2 cut(s)	2473, 2869
BfuI	GTATCC	1 cut(s)	1111
BglII	AGATCT	2 cut(s)	61, 588
BlpI	GCTNAGC	1 cut(s)	746
Bme1390I	CCNGG	3 cut(s)	279, 704, 1430
Bme18I	GGWCC	1 cut(s)	30
BmeT110I	CYCGRG	1 cut(s)	508
BmgT120I	GGNCC	3 cut(s)	30, 1609, 1674
BmiI	GGNNCC	6 cut(s)	193, 438, 701, 1066, 1611, 2087
BmrFI	CCNGG	3 cut(s)	279, 704, 1430
BmrI	ACTGGG	2 cut(s)	1029, 3049
BmuI	ACTGGG	2 cut(s)	1029, 3049
BpiI	GAAGAC	3 cut(s)	75, 213, 1572
BplI	GAGNNNNNCTC	8 cut(s)	734, 766, 1106, 1138, 2596, 2628, 2921, 2953
BpmI	CTGGAG	5 cut(s)	225, 791, 869, 879, 2745
Bpu10I	CCTNAGC	1 cut(s)	2643
Bpu1102I	GCTNAGC	1 cut(s)	746
Bpu14I	TTCGAA	1 cut(s)	44
BpuEI	CTTGAG	5 cut(s)	491, 606, 1530, 1705, 2330
BpuMI	CCSGG	1 cut(s)	1430
Bsa29I	ATCGAT	2 cut(s)	116, 1476
BsaBI	GATNNNNATC	1 cut(s)	2058
BsaI	GGTCTC	1 cut(s)	2106
BsaJI	CCNNGG	4 cut(s)	277, 307, 1561, 2397
BsaWI	WCCGGW	1 cut(s)	2994
Bsc4I	CCNNNNNNNGG	9 cut(s)	170, 283, 409, 940, 1435, 1622, 2096, 3040, 3041
Bse118I	RCCGGY	1 cut(s)	1088
Bse1I	ACTGG	4 cut(s)	919, 1024, 2688, 3044
Bse3DI	GCAATG	2 cut(s)	1616, 2843
Bse8I	GATNNNNATC	1 cut(s)	2058
BseBI	CCWGG	2 cut(s)	279, 704
BseCI	ATCGAT	2 cut(s)	116, 1476
BseDI	CCNNGG	4 cut(s)	277, 307, 1561, 2397
BseJI	GATNNNNATC	1 cut(s)	2058
BseLI	CCNNNNNNNGG	9 cut(s)	170, 283, 409, 940, 1435, 1622, 2096, 3040, 3041
BseMI	GCAATG	2 cut(s)	1616, 2843
BseMII	CTCAG	9 cut(s)	237, 321, 737, 1362, 2202, 2589, 2657, 2829, 3090
BseNI	ACTGG	4 cut(s)	919, 1024, 2688, 3044
BseXI	GCAGC	8 cut(s)	406, 558, 762, 1061, 1495, 1517, 1544, 1869
BseYI	CCCAGC	3 cut(s)	899, 1914, 2025
BsgI	GTGCAG	1 cut(s)	807
Bsh1236I	CGCG	2 cut(s)	1346, 1761
Bsh1285I	CGRYCG	2 cut(s)	53, 2999
BshFI	GGCC	3 cut(s)	1610, 1676, 2797
BshVI	ATCGAT	2 cut(s)	116, 1476
BsiEI	CGRYCG	2 cut(s)	53, 2999
BsiHKAI	GWGCWC	2 cut(s)	752, 3072
BsiHKCI	CYCGRG	1 cut(s)	508
BsiSI	CCGG	3 cut(s)	1089, 1429, 2995
BslFI	GGGAC	2 cut(s)	1891, 3057
BslI	CCNNNNNNNGG	9 cut(s)	170, 283, 409, 940, 1435, 1622, 2096, 3040, 3041
BsmAI	GTCTC	8 cut(s)	146, 339, 683, 2106, 2209, 2220, 2594, 2834
BsmBI	CGTCTC	1 cut(s)	2594
BsmFI	GGGAC	2 cut(s)	1891, 3057
BsnI	GGCC	3 cut(s)	1610, 1676, 2797
Bso31I	GGTCTC	1 cut(s)	2106
BsoBI	CYCGRG	1 cut(s)	508
Bsp119I	TTCGAA	1 cut(s)	44
Bsp1286I	GDGCHC	2 cut(s)	752, 3072
Bsp1720I	GCTNAGC	1 cut(s)	746
Bsp19I	CCATGG	1 cut(s)	307
Bsp68I	TCGCGA	1 cut(s)	1346
BspACI	CCGC	7 cut(s)	846, 931, 1491, 1566, 1712, 2029, 2798
BspANI	GGCC	3 cut(s)	1610, 1676, 2797
BspCNI	CTCAG	9 cut(s)	238, 322, 738, 1363, 2203, 2590, 2656, 2830, 3091
BspDI	ATCGAT	2 cut(s)	116, 1476
BspFNI	CGCG	2 cut(s)	1346, 1761
BspHI	TCATGA	1 cut(s)	1797
BspLI	GGNNCC	6 cut(s)	193, 438, 701, 1066, 1611, 2087
BspMAI	CTGCAG	1 cut(s)	1051
BspMI	ACCTGC	2 cut(s)	2473, 2869
BspPI	GGATC	7 cut(s)	145, 291, 951, 1059, 1072, 2122, 2130
BspQI	GCTCTTC	1 cut(s)	1770
BspT104I	TTCGAA	1 cut(s)	44
BspTNI	GGTCTC	1 cut(s)	2106
BsrBI	CCGCTC	1 cut(s)	2800
BsrDI	GCAATG	2 cut(s)	1616, 2843
BsrFI	RCCGGY	1 cut(s)	1088
BsrI	ACTGG	4 cut(s)	919, 1024, 2688, 3044
BssAI	RCCGGY	1 cut(s)	1088
BssECI	CCNNGG	4 cut(s)	277, 307, 1561, 2397
BssT1I	CCWWGG	2 cut(s)	307, 1561
Bst2UI	CCWGG	2 cut(s)	279, 704
Bst4CI	ACNGT	8 cut(s)	105, 552, 630, 977, 2078, 2203, 2330, 2828
Bst6I	CTCTTC	4 cut(s)	151, 369, 1107, 1770
BstAPI	GCANNNNNTGC	1 cut(s)	854
BstBI	TTCGAA	1 cut(s)	44
BstC8I	GCNNGC	6 cut(s)	499, 897, 1348, 2388, 2526, 3091
BstDSI	CCRYGG	1 cut(s)	307
BstEII	GGTNACC	1 cut(s)	2312
BstFNI	CGCG	2 cut(s)	1346, 1761
BstH2I	RGCGCY	3 cut(s)	533, 1315, 2525
BstHHI	GCGC	7 cut(s)	256, 532, 1314, 1763, 2278, 2524, 2716
BstMAI	GTCTC	8 cut(s)	146, 339, 683, 2106, 2209, 2220, 2594, 2834
BstMCI	CGRYCG	2 cut(s)	53, 2999
BstNI	CCWGG	2 cut(s)	279, 704
BstNSI	RCATGY	2 cut(s)	1660, 2454
BstPI	GGTNACC	1 cut(s)	2312
BstSCI	CCNGG	3 cut(s)	277, 702, 1428
BstSFI	CTRYAG	4 cut(s)	1047, 1317, 1527, 2172
BstUI	CGCG	2 cut(s)	1346, 1761
BstV1I	GCAGC	8 cut(s)	406, 558, 762, 1061, 1495, 1517, 1544, 1869
BstV2I	GAAGAC	3 cut(s)	75, 213, 1572
BstX2I	RGATCY	5 cut(s)	61, 296, 588, 1064, 2114
BstYI	RGATCY	5 cut(s)	61, 296, 588, 1064, 2114
Bsu15I	ATCGAT	2 cut(s)	116, 1476
BsuI	GTATCC	1 cut(s)	1111
BsuRI	GGCC	3 cut(s)	1610, 1676, 2797
BsuTUI	ATCGAT	2 cut(s)	116, 1476
BtgI	CCRYGG	1 cut(s)	307
BtuMI	TCGCGA	1 cut(s)	1346
BveI	ACCTGC	2 cut(s)	2473, 2869
Cac8I	GCNNGC	6 cut(s)	499, 897, 1348, 2388, 2526, 3091
CaiI	CAGNNNCTG	2 cut(s)	1253, 1703
CciI	TCATGA	1 cut(s)	1797
CfoI	GCGC	7 cut(s)	256, 532, 1314, 1763, 2278, 2524, 2716
Cfr10I	RCCGGY	1 cut(s)	1088
Cfr13I	GGNCC	3 cut(s)	30, 1609, 1674
ClaI	ATCGAT	2 cut(s)	116, 1476
CseI	GACGC	2 cut(s)	831, 1888
Csp6I	GTAC	2 cut(s)	1034, 2594
CviQI	GTAC	2 cut(s)	1034, 2594
DraIII	CACNNNGTG	1 cut(s)	2830
EaeI	YGGCCR	1 cut(s)	2795
Eam1104I	CTCTTC	4 cut(s)	151, 369, 1107, 1770
EarI	CTCTTC	4 cut(s)	151, 369, 1107, 1770
EciI	GGCGGA	1 cut(s)	920
Ecl136II	GAGCTC	1 cut(s)	750
Eco130I	CCWWGG	2 cut(s)	307, 1561
Eco24I	GRGCYC	1 cut(s)	752
Eco31I	GGTCTC	1 cut(s)	2106
Eco47I	GGWCC	1 cut(s)	30
Eco47III	AGCGCT	2 cut(s)	531, 1313
Eco53kI	GAGCTC	1 cut(s)	750
Eco57I	CTGAAG	6 cut(s)	972, 1654, 2007, 2229, 2857, 2981
Eco88I	CYCGRG	1 cut(s)	508
Eco91I	GGTNACC	1 cut(s)	2312
EcoICRI	GAGCTC	1 cut(s)	750
EcoO109I	RGGNCCY	1 cut(s)	1609
EcoO65I	GGTNACC	1 cut(s)	2312
EcoRI	GAATTC	3 cut(s)	992, 2655, 3056
EcoRII	CCWGG	2 cut(s)	277, 702
EcoT14I	CCWWGG	2 cut(s)	307, 1561
EcoT22I	ATGCAT	3 cut(s)	202, 1642, 2993
EcoT38I	GRGCYC	1 cut(s)	752
ErhI	CCWWGG	2 cut(s)	307, 1561
Esp3I	CGTCTC	1 cut(s)	2594
FalI	AAGNNNNNCTT	4 cut(s)	130, 162, 1935, 1967
FaqI	GGGAC	2 cut(s)	1891, 3057
FauI	CCCGC	1 cut(s)	1559
FbaI	TGATCA	2 cut(s)	391, 1954
FriOI	GRGCYC	1 cut(s)	752
FspBI	CTAG	9 cut(s)	369, 527, 756, 954, 1424, 1751, 1866, 2160, 3050
GlaI	GCGC	7 cut(s)	255, 531, 1313, 1762, 2277, 2523, 2715
GsaI	CCCAGC	3 cut(s)	903, 1918, 2029
GsuI	CTGGAG	5 cut(s)	225, 791, 869, 879, 2745
HaeII	RGCGCY	3 cut(s)	533, 1315, 2525
HaeIII	GGCC	3 cut(s)	1610, 1676, 2797
HapII	CCGG	3 cut(s)	1089, 1429, 2995
HgaI	GACGC	2 cut(s)	831, 1888
HhaI	GCGC	7 cut(s)	256, 532, 1314, 1763, 2278, 2524, 2716
Hin6I	GCGC	7 cut(s)	254, 530, 1312, 1761, 2276, 2522, 2714
HinP1I	GCGC	7 cut(s)	254, 530, 1312, 1761, 2276, 2522, 2714
HincII	GTYRAC	2 cut(s)	108, 808
HindII	GTYRAC	2 cut(s)	108, 808
HindIII	AAGCTT	2 cut(s)	1645, 2963
HpaI	GTTAAC	2 cut(s)	108, 808
HpaII	CCGG	3 cut(s)	1089, 1429, 2995
HphI	GGTGA	4 cut(s)	93, 2199, 2816, 2893
Hpy166II	GTNNAC	4 cut(s)	108, 808, 1909, 2326
Hpy8I	GTNNAC	4 cut(s)	108, 808, 1909, 2326
HpyCH4III	ACNGT	8 cut(s)	105, 552, 630, 977, 2078, 2203, 2330, 2828
HpyCH4IV	ACGT	2 cut(s)	315, 781
HpySE526I	ACGT	2 cut(s)	315, 781
HspAI	GCGC	7 cut(s)	254, 530, 1312, 1761, 2276, 2522, 2714
Ksp22I	TGATCA	2 cut(s)	391, 1954
KspAI	GTTAAC	2 cut(s)	108, 808
LguI	GCTCTTC	1 cut(s)	1770
Lsp1109I	GCAGC	8 cut(s)	406, 558, 762, 1061, 1495, 1517, 1544, 1869
MaeI	CTAG	9 cut(s)	369, 527, 756, 954, 1424, 1751, 1866, 2160, 3050
MaeII	ACGT	2 cut(s)	315, 781
MaeIII	GTNAC	6 cut(s)	546, 630, 949, 1723, 2312, 3000
MbiI	CCGCTC	1 cut(s)	2800
MfeI	CAATTG	2 cut(s)	1452, 1512
MflI	RGATCY	5 cut(s)	61, 296, 588, 1064, 2114
MhlI	GDGCHC	2 cut(s)	752, 3072
MlyI	GAGTC	4 cut(s)	341, 1354, 1904, 2222
MmeI	TCCRAC	3 cut(s)	324, 2337, 3033
Mph1103I	ATGCAT	3 cut(s)	202, 1642, 2993
MroXI	GAANNNNTTC	3 cut(s)	1461, 1992, 2406
MslI	CAYNNNNRTG	3 cut(s)	2369, 2664, 2721
MspA1I	CMGCKG	4 cut(s)	245, 1493, 1508, 2029
MspI	CCGG	3 cut(s)	1089, 1429, 2995
MspR9I	CCNGG	3 cut(s)	279, 704, 1430
MunI	CAATTG	2 cut(s)	1452, 1512
MvaI	CCWGG	2 cut(s)	279, 704
MvnI	CGCG	2 cut(s)	1346, 1761
NciI	CCSGG	1 cut(s)	1430
NcoI	CCATGG	1 cut(s)	307
NlaIV	GGNNCC	6 cut(s)	193, 438, 701, 1066, 1611, 2087
NmuCI	GTSAC	1 cut(s)	546
NruI	TCGCGA	1 cut(s)	1346
NsiI	ATGCAT	3 cut(s)	202, 1642, 2993
NspI	RCATGY	2 cut(s)	1660, 2454
NspV	TTCGAA	1 cut(s)	44
PaeR7I	CTCGAG	1 cut(s)	508
PagI	TCATGA	1 cut(s)	1797
PaqCI	CACCTGC	1 cut(s)	2869
PciI	ACATGT	1 cut(s)	1656
PciSI	GCTCTTC	1 cut(s)	1770
PcsI	WCGNNNNNNNCGW	1 cut(s)	50
PdmI	GAANNNNTTC	3 cut(s)	1461, 1992, 2406
PflFI	GACNNNGTC	1 cut(s)	1363
PfoI	TCCNGGA	1 cut(s)	702
Ple19I	CGATCG	1 cut(s)	53
PleI	GAGTC	4 cut(s)	340, 1354, 1904, 2221
PpsI	GAGTC	4 cut(s)	340, 1354, 1904, 2221
PscI	ACATGT	1 cut(s)	1656
PshBI	ATTAAT	1 cut(s)	762
PsiI	TTATAA	1 cut(s)	1829
Psp124BI	GAGCTC	1 cut(s)	752
Psp6I	CCWGG	2 cut(s)	277, 702
PspEI	GGTNACC	1 cut(s)	2312
PspFI	CCCAGC	3 cut(s)	899, 1914, 2025
PspGI	CCWGG	2 cut(s)	277, 702
PspN4I	GGNNCC	6 cut(s)	193, 438, 701, 1066, 1611, 2087
PspPI	GGNCC	3 cut(s)	30, 1609, 1674
PstI	CTGCAG	1 cut(s)	1051
PstNI	CAGNNNCTG	2 cut(s)	1253, 1703
PsuI	RGATCY	5 cut(s)	61, 296, 588, 1064, 2114
PsyI	GACNNNGTC	1 cut(s)	1363
PvuI	CGATCG	1 cut(s)	53
PvuII	CAGCTG	2 cut(s)	245, 1508
RruI	TCGCGA	1 cut(s)	1346
RsaI	GTAC	2 cut(s)	1035, 2595
RsaNI	GTAC	2 cut(s)	1034, 2594
RseI	CAYNNNNRTG	3 cut(s)	2369, 2664, 2721
SacI	GAGCTC	1 cut(s)	752
SapI	GCTCTTC	1 cut(s)	1770
Sau96I	GGNCC	3 cut(s)	30, 1609, 1674
SchI	GAGTC	4 cut(s)	341, 1354, 1904, 2222
ScrFI	CCNGG	3 cut(s)	279, 704, 1430
SduI	GDGCHC	2 cut(s)	752, 3072
SfcI	CTRYAG	4 cut(s)	1047, 1317, 1527, 2172
Sfr274I	CTCGAG	1 cut(s)	508
SfuI	TTCGAA	1 cut(s)	44
SinI	GGWCC	1 cut(s)	30
SlaI	CTCGAG	1 cut(s)	508
SmiMI	CAYNNNNRTG	3 cut(s)	2369, 2664, 2721
SmlI	CTYRAG	6 cut(s)	470, 508, 621, 1545, 1684, 2345
SmoI	CTYRAG	6 cut(s)	470, 508, 621, 1545, 1684, 2345
SpeI	ACTAGT	1 cut(s)	953
SsiI	CCGC	7 cut(s)	846, 931, 1491, 1566, 1712, 2029, 2798
SspI	AATATT	1 cut(s)	2340
SspMI	CTAG	9 cut(s)	369, 527, 756, 954, 1424, 1751, 1866, 2160, 3050
SstI	GAGCTC	1 cut(s)	752
StyD4I	CCNGG	3 cut(s)	277, 702, 1428
StyI	CCWWGG	2 cut(s)	307, 1561
TaaI	ACNGT	8 cut(s)	105, 552, 630, 977, 2078, 2203, 2330, 2828
TaiI	ACGT	2 cut(s)	318, 784
TatI	WGTACW	1 cut(s)	1033
TauI	GCSGC	3 cut(s)	1493, 1715, 2800
TseFI	GTSAC	1 cut(s)	546
TseI	GCWGC	8 cut(s)	419, 571, 775, 1049, 1505, 1508, 1532, 1882
Tsp45I	GTSAC	1 cut(s)	546
TspGWI	ACGGA	4 cut(s)	183, 492, 1853, 2295
Tth111I	GACNNNGTC	1 cut(s)	1363
VpaK11BI	GGWCC	1 cut(s)	30
VspI	ATTAAT	1 cut(s)	762
XapI	RAATTY	6 cut(s)	712, 992, 1823, 1991, 2655, 3056
XceI	RCATGY	2 cut(s)	1660, 2454
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1903
XhoI	CTCGAG	1 cut(s)	508
XmnI	GAANNNNTTC	3 cut(s)	1461, 1992, 2406
XspI	CTAG	9 cut(s)	369, 527, 756, 954, 1424, 1751, 1866, 2160, 3050
Zsp2I	ATGCAT	3 cut(s)	202, 1642, 2993

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.