Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0005460

Physical Location & Seq

Genomic Coordinates Forward (+)

705343 .. 713771

Gene Structure 8429 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G000930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3042 bp

ATGAGCAGCAATGGTTCTAAGAGAACGGTGACGACGGCCATGGACTCCGTGAGGTCCTCCCACACCCGATCCAACTCCATATCCGACCCGAATTCCAGCACCAGCACCAGCCAGAGCCTCGCCTCCATCCTCAACAACCCCAATGCCTCCGACTCCTCCTCCTGGGCCGCCTGGTGGTCCTCCTCCGCCTCCGTCGCCCCGCCCGAGTTCCTCCCCCTCCTCCCCAAATCCGCATCCGACTCCCTGACCCGATCCGACTTCCTCCCCTACATCTCCTCCATCTCCGATCACTACAACCGCTTCGAGGACATCCTCAACCACGTCAAGAAGGAGAGCCTCGACTTCGACTCCATCGGCGGCCAGGGCGAAGCTCTCGTCGCTTGTCTCCGCGAAGTCCCCGCCCTCTATTTCAAAGAGGACTTCGCATTGGAAGACGGCGCCACTTTCCGGTCCGCCTGCCCCTTCTCCGGCGTCTCCGAGAACCTCGTCCTCCAGGAGAAGCTCTCGCATTACTTGGACGTGGTGGAGCTTCACCTGGTGAAGGAGATCTCGCTCCGATCAAACTCCTTCTTCGAGGCTCAGGGCCAGTTGCAGGACTTGAATGTGAAGATCGTTGAGGGATGCAATCGGATTCGGGAGCTTAAGGAGACCATTTTGCTCTTGGATGTGGATTTGGTCGACTCTGCTAGGCAGATTCAGGAGCTCAATGTTACCAGGAGTAATTTGTTGGCTCTTCAACAGAAGCTGAGGCTGATTCTCTATGTCAATCAAGCTCTTTCGGCGCTCAAACTGCTTGTTGCCTCTGCAGATTGTGCTGGAGCTTTGGATGTCACAGATGACCTGCAGCATTTGCTGGACGGGGATGAACTTACTGGTCTACATTGCTTTCATCACCTCAGGGATCGTGTAGCAGCGTCAATTGAATCCATAAACAGCATTCTTTCTGCAGAATTTATGCGTGCTTCAATACATGATGCTGGAGATACAGACGCTATAATTATATCTAAAGCGAAAGCAAGGGCATCCATTCTCATGAACGGGGAAGATGGTGAAGTTAAGTTGGACGATGAAGAAACCTCCAATTATCAAGATCGTCTTCTTCCTATCATCATTGGATTGCTTAGAACAGCTAAGCTCCCTTCTGTGTTGAGGCTATATCGTGACCAACTTACGGCTGATATGAAGACTGCTATTAAGAATGCAGTTGCAGAGCTGCTTCCCATTCTTGTTTCCCGACCTCTGGAATCCGATTTTACACCTGGAGAGCGAGTTGCAGATGCAGACGGCATAGGTGCGTCACTTGCAAGCAAGTTGAGGAGCCTGTCATCTGAAAGCTTTGTTCAACTTTTAAGTGCTATTTTTCTGATTGTAAGGGCACATCTAGTACGGTCTGCTGAAGTGAAGAAGGCTATTGAGTGGATTATGTGCAACCTTGATGGTCACTATGCAGCTGATTCAGTAGCTGCTGCACTTGCAATTGGCGCTGAGGCTGCAGAAACAGCTCAAGAAAGTGATGGCCAAGGTGGCTTGCTCGTGTCGTATTCATCTCCTAGAGTTGCTGCAAAGGCCCTTTCATTCCAAGGAAAAGCAAATGATGCAACAAGTCCTTCTACTACATCTAAGAACTTTAGAGCTGATGTGTTACGAGAAAACACAGAAGCCGTTGTTGCAGCATGTGATGCTGCTCAGGGAAGATGGGCAAAGCTACTTGGGGTTCGTGCTCTTCTTCATCCGAAGTTGAGATTACAGGAGTTTTTGAGCATATATAATATCACACAAGAGTTTATAACTGCTACAGAGAAGGTAGGGGGAAGGCCGGGATTTAGCATCAGAGGAACACTACAATCACAGGCCAAAGCCTTCCTCGATTTTCAGCATGAATCACGAATGACCAAGATTAAGGCAGTTCTAGACCAAGAAACGTGGGTGGAAGTAGATGTTCCTGATGAATTTCAGTTTATTGTCAATTCATTATTTTGTTCTGAAGCATTGGTAGCCGAGAACCTGGATGCTGGCCATGGTAGTACGGAAACAAGCTACAATGAGGTGGATACAGGACCATCAATTACAGAAAAGCAAATTAAGCAAACTGATTCAACTGAATTATCTACGGATATGACTGGAAACAATAAGTCTACATCTGCTGATGGAGCTGAAAAGAATAAGGCTGATGTTACAAATTCTGTGGCTCAGCACAACCACAGCAACATGAAGGAGAGGGGAAAATCAACTTCCCAAATCTTATCTTACAAAGGTGTTGGTTTTCACATGGTAAACTGTGGCCTGATATTGATGAAGATGTTGTCAGAGTATATTGATATGAACAATTTTTTTCCAGTACTGTCCTCAGAAGTTGTTCATCGTATCGTTGAAATTTTAAAATTTTTCAATACAAGAACATGTCAGCTTGTTCTTGGTGCTGGTGCCATGCAGGTATCTGGTCTGAAGTCGATCACTTCTAAACACTTGGCCTTGGCAAGTCAAGTCATCAGTTTTACATATGCTATTATTCCTGAACTCAGGCAAATTCTTTTTCTGAAAGTACCTGAGACAAGAAAGGCAATGTTGCTATCTGAAATTGATCGAGTAGCTCAGGATTATAAGGTTCATAGAGATGAAATACATACAAAGCTGGTTCAGATCATGAGAGAAAGGTTATTGGTTCATCTACGTGGGTTGCCTCAAATTGTAGAGAGCTGGAATAGACCAGAAGATGCTGACCTACAGCCCAGTCAATTTGCTCGATCACTTACCAAGGAAGTTGGATACTTGCAACGTGTTCTAACTCGAACTTTACATGAGGTCGATGTTCAAGCAATTTTCAGGCAAGTGATTATAATTTTTCATTCACAAATTTCAGAAGCATTTTCCCGCTTAGAGATCAGCACACCACAAGCCAAGGACAGGCTCTGTCGTGATGTTAAGCACATTCTTGGATGCATTCGATCTTTGCCTTCTGATAAACTGAGTGAATCTGGTACGCCAAACTGGGGTCAACTAGATGAATTCTTGGTGCAGAGATTTGGGTCAGAAGCTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000149 GO:0000938 GO:0001894 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005802 GO:0006810 GO:0006892 GO:0006896 GO:0006996 GO:0007010 GO:0007034 GO:0007041 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016482 GO:0019905 GO:0031090 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032501 GO:0032588 GO:0032991 GO:0040008 GO:0042147 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045103 GO:0045104 GO:0046907 GO:0048193 GO:0048471 GO:0048871 GO:0048872 GO:0048873 GO:0050789 GO:0050879 GO:0050881 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060052 GO:0060249 GO:0065007 GO:0065008 GO:0070013 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps54

Protein Analysis

1013

Amino Acids

111.62

Weight (kDa)

5.61

Isoelectric Point (pI)

40.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps54	PF07928	755 - 885	8.1e-47	Vps54-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005460)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19490 AT4G19490
fragaria_vesca	FvH4_6g01010 FvH4_6g01010 FvH4_6g01010
malus_domestica	MD04G1237800.v1.1 MD12G1256000.v1.1
prunus_persica	Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1
pyrus_communis	pycom04g21100 pycom12g23480
rosa_chinensis	RchiOBHm_Chr3g0448451
rosa_laevigata	RLG00000000687 RLG00000025905
rosa_multiflora	Rmu_sc0003308.1_g000012 Rmu_sc0011979.1_g000005
rosa_roxburghii	Rroxscaffold_6G00426680
rosa_rugosa	Rorug02G0612500 Rorug02G0612600 Rorug02G0612600 Rorug07G0334800
rosa_samantha	Rh3AG012000 Rh3BG011800 Rh3CG011500 Rh3DG012200 Rh7BG461900
rosa_wichuraiana	Rw3G000930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1787, 2601, 2837
Acc36I	ACCTGC	2 cut(s)	849, 2422
AccB1I	GGYRCC	2 cut(s)	437, 2423
AccI	GTMKAC	3 cut(s)	678, 877, 2135
AccII	CGCG	1 cut(s)	390
AclWI	GGATC	3 cut(s)	63, 246, 909
AcoI	YGGCCR	4 cut(s)	36, 358, 1516, 2014
AcsI	RAATTY	9 cut(s)	91, 950, 1949, 2179, 2373, 2381, 2526, 2853, 3005
AcuI	CTGAAG	3 cut(s)	1416, 2004, 2465
AcyI	GRCGYC	2 cut(s)	438, 471
AdeI	CACNNNGTG	1 cut(s)	538
AfaI	GTAC	5 cut(s)	1386, 2026, 2340, 2544, 2980
AflII	CTTAAG	1 cut(s)	641
AflIII	ACRYGT	2 cut(s)	2399, 2776
AjiI	CACGTC	2 cut(s)	322, 520
AjnI	CCWGG	8 cut(s)	161, 170, 360, 492, 534, 713, 1258, 2004
Alw21I	GWGCWC	2 cut(s)	705, 1723
Alw26I	GTCTC	4 cut(s)	389, 478, 641, 2543
AlwI	GGATC	3 cut(s)	63, 246, 909
AlwNI	CAGNNNCTG	3 cut(s)	745, 1463, 2910
Ama87I	CYCGRG	1 cut(s)	203
ApoI	RAATTY	9 cut(s)	91, 950, 1949, 2179, 2373, 2381, 2526, 2853, 3005
ArsI	GACNNNNNNTTYG	2 cut(s)	360, 392
Asp700I	GAANNNNTTC	1 cut(s)	2355
AspLEI	GCGC	3 cut(s)	440, 784, 1484
AspS9I	GGNCC	7 cut(s)	54, 165, 177, 450, 583, 1567, 2057
AsuC2I	CCSGG	1 cut(s)	1818
AsuHPI	GGTGA	5 cut(s)	40, 524, 550, 884, 1061
AvaI	CYCGRG	1 cut(s)	203
AvaII	GGWCC	4 cut(s)	54, 177, 450, 2057
AxyI	CCTNAGG	1 cut(s)	896
BaeGI	GKGCMC	1 cut(s)	1378
BalI	TGGCCA	2 cut(s)	1518, 2016
BanI	GGYRCC	2 cut(s)	437, 2423
BanII	GRGCYC	1 cut(s)	705
BauI	CACGAG	1 cut(s)	1532
BbsI	GAAGAC	3 cut(s)	438, 1088, 1190
Bbv12I	GWGCWC	2 cut(s)	705, 1723
BbvCI	CCTCAGC	2 cut(s)	746, 1485
BccI	CCATC	9 cut(s)	134, 287, 359, 1040, 1430, 1508, 1689, 2068, 2141
BceAI	ACGGC	5 cut(s)	51, 451, 1188, 1300, 1646
BciT130I	CCWGG	8 cut(s)	163, 172, 362, 494, 536, 715, 1260, 2006
BciVI	GTATCC	2 cut(s)	2044, 2759
BcnI	CCSGG	1 cut(s)	1818
BcoDI	GTCTC	4 cut(s)	389, 478, 641, 2543
BfaI	CTAG	6 cut(s)	687, 1382, 1551, 1910, 2999, 3036
BfmI	CTRYAG	6 cut(s)	804, 842, 945, 1491, 1794, 2723
BfoI	RGCGCY	3 cut(s)	441, 785, 1485
BfrI	CTTAAG	1 cut(s)	641
BfuAI	ACCTGC	2 cut(s)	849, 2422
BfuI	GTATCC	2 cut(s)	2044, 2759
BglI	GCCNNNNNGGC	1 cut(s)	1524
BglII	AGATCT	1 cut(s)	546
BlpI	GCTNAGC	2 cut(s)	1131, 2190
BmcAI	AGTACT	1 cut(s)	2340
Bme1390I	CCNGG	9 cut(s)	163, 172, 362, 494, 536, 715, 1260, 1818, 2006
Bme18I	GGWCC	4 cut(s)	54, 177, 450, 2057
BmeT110I	CYCGRG	1 cut(s)	203
BmgBI	CACGTC	2 cut(s)	322, 520
BmgT120I	GGNCC	7 cut(s)	54, 165, 177, 450, 583, 1567, 2057
BmiI	GGNNCC	3 cut(s)	439, 1319, 2425
BmrFI	CCNGG	9 cut(s)	163, 172, 362, 494, 536, 715, 1260, 1818, 2006
BmrI	ACTGGG	2 cut(s)	2724, 2998
BmuI	ACTGGG	2 cut(s)	2724, 2998
BpiI	GAAGAC	3 cut(s)	438, 1088, 1190
BplI	GAGNNNNNCTC	2 cut(s)	488, 520
BpmI	CTGGAG	4 cut(s)	476, 837, 999, 1281
Bpu10I	CCTNAGC	5 cut(s)	579, 746, 1485, 1686, 2592
Bpu1102I	GCTNAGC	2 cut(s)	1131, 2190
BpuEI	CTTGAG	1 cut(s)	1488
BpuMI	CCSGG	1 cut(s)	1818
BsaAI	YACGTR	1 cut(s)	2672
BsaHI	GRCGYC	2 cut(s)	438, 471
BsaI	GGTCTC	1 cut(s)	641
BsaJI	CCNNGG	9 cut(s)	39, 162, 361, 1519, 1579, 2017, 2472, 2754, 2898
BsaWI	WCCGGW	1 cut(s)	447
BsaXI	ACNNNNNCTCC	8 cut(s)	143, 173, 260, 290, 332, 362, 1254, 1284
Bse1I	ACTGG	6 cut(s)	586, 877, 2125, 2336, 2730, 2993
Bse21I	CCTNAGG	1 cut(s)	896
Bse3DI	GCAATG	3 cut(s)	16, 880, 2568
BseBI	CCWGG	8 cut(s)	163, 172, 362, 494, 536, 715, 1260, 2006
BseDI	CCNNGG	9 cut(s)	39, 162, 361, 1519, 1579, 2017, 2472, 2754, 2898
BseMI	GCAATG	3 cut(s)	16, 880, 2568
BseNI	ACTGG	6 cut(s)	586, 877, 2125, 2336, 2730, 2993
BseRI	GAGGAG	6 cut(s)	145, 148, 172, 209, 265, 1330
BseSI	GKGCMC	1 cut(s)	1378
BsgI	GTGCAG	2 cut(s)	1452, 3035
Bsh1236I	CGCG	1 cut(s)	390
BshNI	GGYRCC	2 cut(s)	437, 2423
BsiHKAI	GWGCWC	2 cut(s)	705, 1723
BsiHKCI	CYCGRG	1 cut(s)	203
BsiSI	CCGG	3 cut(s)	448, 468, 1817
BslFI	GGGAC	1 cut(s)	380
BsmAI	GTCTC	4 cut(s)	389, 478, 641, 2543
BsmBI	CGTCTC	1 cut(s)	478
BsmFI	GGGAC	1 cut(s)	380
BsmI	GAATGC	3 cut(s)	936, 1204, 2940
Bso31I	GGTCTC	1 cut(s)	641
BsoBI	CYCGRG	1 cut(s)	203
Bsp1286I	GDGCHC	3 cut(s)	705, 1378, 1723
Bsp1720I	GCTNAGC	2 cut(s)	1131, 2190
Bsp19I	CCATGG	2 cut(s)	39, 2017
BspFNI	CGCG	1 cut(s)	390
BspHI	TCATGA	2 cut(s)	1032, 2643
BspLI	GGNNCC	3 cut(s)	439, 1319, 2425
BspMAI	CTGCAG	4 cut(s)	808, 846, 949, 1495
BspMI	ACCTGC	2 cut(s)	849, 2422
BspPI	GGATC	3 cut(s)	63, 246, 909
BspQI	GCTCTTC	2 cut(s)	738, 1728
BspT107I	GGYRCC	2 cut(s)	437, 2423
BspTI	CTTAAG	1 cut(s)	641
BspTNI	GGTCTC	1 cut(s)	641
BsrDI	GCAATG	3 cut(s)	16, 880, 2568
BsrI	ACTGG	6 cut(s)	586, 877, 2125, 2336, 2730, 2993
BssECI	CCNNGG	9 cut(s)	39, 162, 361, 1519, 1579, 2017, 2472, 2754, 2898
BssNI	GRCGYC	2 cut(s)	438, 471
BssSI	CACGAG	1 cut(s)	1532
BssT1I	CCWWGG	7 cut(s)	39, 1519, 1579, 2017, 2472, 2754, 2898
Bst2BI	CACGAG	1 cut(s)	1532
Bst2UI	CCWGG	8 cut(s)	163, 172, 362, 494, 536, 715, 1260, 2006
Bst4CI	ACNGT	4 cut(s)	28, 1389, 2279, 2343
Bst6I	CTCTTC	2 cut(s)	738, 1728
BstACI	GRCGYC	2 cut(s)	438, 471
BstAFI	CTTAAG	1 cut(s)	641
BstAPI	GCANNNNNTGC	4 cut(s)	812, 850, 1595, 1679
BstBAI	YACGTR	1 cut(s)	2672
BstC8I	GCNNGC	5 cut(s)	457, 960, 1306, 1529, 2014
BstDSI	CCRYGG	2 cut(s)	39, 2017
BstENI	CCTNNNNNAGG	1 cut(s)	539
BstFNI	CGCG	1 cut(s)	390
BstH2I	RGCGCY	3 cut(s)	441, 785, 1485
BstHHI	GCGC	3 cut(s)	440, 784, 1484
BstMAI	GTCTC	4 cut(s)	389, 478, 641, 2543
BstNI	CCWGG	8 cut(s)	163, 172, 362, 494, 536, 715, 1260, 2006
BstNSI	RCATGY	2 cut(s)	1677, 2403
BstSCI	CCNGG	9 cut(s)	161, 170, 360, 492, 534, 713, 1258, 1816, 2004
BstSFI	CTRYAG	6 cut(s)	804, 842, 945, 1491, 1794, 2723
BstSLI	GKGCMC	1 cut(s)	1378
BstUI	CGCG	1 cut(s)	390
BstV2I	GAAGAC	3 cut(s)	438, 1088, 1190
BstX2I	RGATCY	1 cut(s)	546
BstYI	RGATCY	1 cut(s)	546
Bsu36I	CCTNAGG	1 cut(s)	896
BsuI	GTATCC	2 cut(s)	2044, 2759
BtgI	CCRYGG	2 cut(s)	39, 2017
BtrI	CACGTC	2 cut(s)	322, 520
BveI	ACCTGC	2 cut(s)	849, 2422
Cac8I	GCNNGC	5 cut(s)	457, 960, 1306, 1529, 2014
CaiI	CAGNNNCTG	3 cut(s)	745, 1463, 2910
CciI	TCATGA	2 cut(s)	1032, 2643
CfoI	GCGC	3 cut(s)	440, 784, 1484
Cfr13I	GGNCC	7 cut(s)	54, 165, 177, 450, 583, 1567, 2057
CpoI	CGGWCCG	1 cut(s)	450
CseI	GACGC	4 cut(s)	460, 903, 998, 1284
CsiI	ACCWGGT	1 cut(s)	534
Csp6I	GTAC	5 cut(s)	1385, 2025, 2339, 2543, 2979
CspCI	CAANNNNNGTGG	4 cut(s)	1904, 1939, 2166, 2201
CspI	CGGWCCG	1 cut(s)	450
CviQI	GTAC	5 cut(s)	1385, 2025, 2339, 2543, 2979
DinI	GGCGCC	1 cut(s)	439
DraI	TTTAAA	1 cut(s)	2379
DraIII	CACNNNGTG	1 cut(s)	538
EaeI	YGGCCR	4 cut(s)	36, 358, 1516, 2014
Eam1104I	CTCTTC	2 cut(s)	738, 1728
EarI	CTCTTC	2 cut(s)	738, 1728
EciI	GGCGGA	2 cut(s)	175, 442
Ecl136II	GAGCTC	1 cut(s)	703
Eco130I	CCWWGG	7 cut(s)	39, 1519, 1579, 2017, 2472, 2754, 2898
Eco24I	GRGCYC	1 cut(s)	705
Eco31I	GGTCTC	1 cut(s)	641
Eco47I	GGWCC	4 cut(s)	54, 177, 450, 2057
Eco53kI	GAGCTC	1 cut(s)	703
Eco57I	CTGAAG	3 cut(s)	1416, 2004, 2465
Eco81I	CCTNAGG	1 cut(s)	896
Eco88I	CYCGRG	1 cut(s)	203
EcoICRI	GAGCTC	1 cut(s)	703
EcoNI	CCTNNNNNAGG	1 cut(s)	539
EcoO109I	RGGNCCY	2 cut(s)	54, 1567
EcoRI	GAATTC	2 cut(s)	91, 3005
EcoRII	CCWGG	8 cut(s)	161, 170, 360, 492, 534, 713, 1258, 2004
EcoT14I	CCWWGG	7 cut(s)	39, 1519, 1579, 2017, 2472, 2754, 2898
EcoT22I	ATGCAT	1 cut(s)	2942
EcoT38I	GRGCYC	1 cut(s)	705
EgeI	GGCGCC	1 cut(s)	439
EheI	GGCGCC	1 cut(s)	439
ErhI	CCWWGG	7 cut(s)	39, 1519, 1579, 2017, 2472, 2754, 2898
Esp3I	CGTCTC	1 cut(s)	478
FalI	AAGNNNNNCTT	2 cut(s)	1080, 1112
FaqI	GGGAC	1 cut(s)	380
FauI	CCCGC	3 cut(s)	207, 406, 2879
FauNDI	CATATG	1 cut(s)	2500
FblI	GTMKAC	3 cut(s)	678, 877, 2135
FriOI	GRGCYC	1 cut(s)	705
FspBI	CTAG	6 cut(s)	687, 1382, 1551, 1910, 2999, 3036
GlaI	GCGC	3 cut(s)	439, 783, 1483
GsuI	CTGGAG	4 cut(s)	476, 837, 999, 1281
HaeII	RGCGCY	3 cut(s)	441, 785, 1485
HapII	CCGG	3 cut(s)	448, 468, 1817
HgaI	GACGC	4 cut(s)	460, 903, 998, 1284
HhaI	GCGC	3 cut(s)	440, 784, 1484
Hin1I	GRCGYC	2 cut(s)	438, 471
Hin6I	GCGC	3 cut(s)	438, 782, 1482
HinP1I	GCGC	3 cut(s)	438, 782, 1482
HincII	GTYRAC	2 cut(s)	679, 2996
HindII	GTYRAC	2 cut(s)	679, 2996
HindIII	AAGCTT	1 cut(s)	1333
HpaII	CCGG	3 cut(s)	448, 468, 1817
HphI	GGTGA	5 cut(s)	40, 524, 550, 884, 1061
Hpy166II	GTNNAC	5 cut(s)	679, 878, 2136, 2275, 2996
Hpy8I	GTNNAC	5 cut(s)	679, 878, 2136, 2275, 2996
Hpy99I	CGWCG	3 cut(s)	37, 197, 380
HpyCH4III	ACNGT	4 cut(s)	28, 1389, 2279, 2343
HpyCH4IV	ACGT	5 cut(s)	321, 519, 1922, 2671, 2776
HpySE526I	ACGT	5 cut(s)	321, 519, 1922, 2671, 2776
Hsp92I	GRCGYC	2 cut(s)	438, 471
HspAI	GCGC	3 cut(s)	438, 782, 1482
KasI	GGCGCC	1 cut(s)	437
LguI	GCTCTTC	2 cut(s)	738, 1728
LmnI	GCTCC	8 cut(s)	526, 558, 637, 700, 818, 1140, 1317, 2150
MabI	ACCWGGT	1 cut(s)	534
MaeI	CTAG	6 cut(s)	687, 1382, 1551, 1910, 2999, 3036
MaeII	ACGT	5 cut(s)	321, 519, 1922, 2671, 2776
MaeIII	GTNAC	8 cut(s)	28, 709, 829, 1160, 1296, 1439, 1641, 2173
MfeI	CAATTG	2 cut(s)	918, 1476
MflI	RGATCY	1 cut(s)	546
MhlI	GDGCHC	3 cut(s)	705, 1378, 1723
MlsI	TGGCCA	2 cut(s)	1518, 2016
MluNI	TGGCCA	2 cut(s)	1518, 2016
Mly113I	GGCGCC	1 cut(s)	438
MlyI	GAGTC	5 cut(s)	38, 146, 233, 341, 674
MmeI	TCCRAC	7 cut(s)	96, 108, 174, 261, 279, 1041, 2743
Mox20I	TGGCCA	2 cut(s)	1518, 2016
Mph1103I	ATGCAT	1 cut(s)	2942
MroXI	GAANNNNTTC	1 cut(s)	2355
MscI	TGGCCA	2 cut(s)	1518, 2016
MseI	TTAA	9 cut(s)	642, 1056, 1194, 1349, 1899, 2082, 2378, 2922, 3040
MslI	CAYNNNNRTG	3 cut(s)	1031, 2613, 2670
Msp20I	TGGCCA	2 cut(s)	1518, 2016
MspA1I	CMGCKG	1 cut(s)	1451
MspCI	CTTAAG	1 cut(s)	641
MspI	CCGG	3 cut(s)	448, 468, 1817
MspR9I	CCNGG	9 cut(s)	163, 172, 362, 494, 536, 715, 1260, 1818, 2006
MunI	CAATTG	2 cut(s)	918, 1476
Mva1269I	GAATGC	3 cut(s)	936, 1204, 2940
MvaI	CCWGG	8 cut(s)	163, 172, 362, 494, 536, 715, 1260, 2006
MvnI	CGCG	1 cut(s)	390
NarI	GGCGCC	1 cut(s)	438
NciI	CCSGG	1 cut(s)	1818
NcoI	CCATGG	2 cut(s)	39, 2017
NdeI	CATATG	1 cut(s)	2500
NlaIV	GGNNCC	3 cut(s)	439, 1319, 2425
NmeAIII	GCCGAG	1 cut(s)	2023
NmuCI	GTSAC	5 cut(s)	28, 829, 1160, 1296, 1439
NsiI	ATGCAT	1 cut(s)	2942
NspI	RCATGY	2 cut(s)	1677, 2403
PagI	TCATGA	2 cut(s)	1032, 2643
PciI	ACATGT	1 cut(s)	2399
PciSI	GCTCTTC	2 cut(s)	738, 1728
PcsI	WCGNNNNNNNCGW	1 cut(s)	201
PctI	GAATGC	3 cut(s)	936, 1204, 2940
PdmI	GAANNNNTTC	1 cut(s)	2355
PfeI	GAWTC	9 cut(s)	631, 694, 754, 923, 1244, 1454, 1880, 2093, 2972
PfoI	TCCNGGA	1 cut(s)	492
PleI	GAGTC	5 cut(s)	38, 146, 233, 341, 674
PluTI	GGCGCC	1 cut(s)	441
PpsI	GAGTC	5 cut(s)	38, 146, 233, 341, 674
Ppu21I	YACGTR	1 cut(s)	2672
PpuMI	RGGWCCY	1 cut(s)	54
PscI	ACATGT	1 cut(s)	2399
PsiI	TTATAA	3 cut(s)	1787, 2601, 2837
Psp124BI	GAGCTC	1 cut(s)	705
Psp5II	RGGWCCY	1 cut(s)	54
Psp6I	CCWGG	8 cut(s)	161, 170, 360, 492, 534, 713, 1258, 2004
PspGI	CCWGG	8 cut(s)	161, 170, 360, 492, 534, 713, 1258, 2004
PspN4I	GGNNCC	3 cut(s)	439, 1319, 2425
PspPI	GGNCC	7 cut(s)	54, 165, 177, 450, 583, 1567, 2057
PspPPI	RGGWCCY	1 cut(s)	54
PstI	CTGCAG	4 cut(s)	808, 846, 949, 1495
PstNI	CAGNNNCTG	3 cut(s)	745, 1463, 2910
PsuI	RGATCY	1 cut(s)	546
PvuII	CAGCTG	1 cut(s)	1451
RsaI	GTAC	5 cut(s)	1386, 2026, 2340, 2544, 2980
RsaNI	GTAC	5 cut(s)	1385, 2025, 2339, 2543, 2979
RseI	CAYNNNNRTG	3 cut(s)	1031, 2613, 2670
Rsr2I	CGGWCCG	1 cut(s)	450
RsrII	CGGWCCG	1 cut(s)	450
SacI	GAGCTC	1 cut(s)	705
SalI	GTCGAC	1 cut(s)	677
SapI	GCTCTTC	2 cut(s)	738, 1728
SaqAI	TTAA	9 cut(s)	642, 1056, 1194, 1349, 1899, 2082, 2378, 2922, 3040
Sau96I	GGNCC	7 cut(s)	54, 165, 177, 450, 583, 1567, 2057
ScaI	AGTACT	1 cut(s)	2340
SchI	GAGTC	5 cut(s)	38, 146, 233, 341, 674
ScrFI	CCNGG	9 cut(s)	163, 172, 362, 494, 536, 715, 1260, 1818, 2006
SduI	GDGCHC	3 cut(s)	705, 1378, 1723
SexAI	ACCWGGT	1 cut(s)	534
SfcI	CTRYAG	6 cut(s)	804, 842, 945, 1491, 1794, 2723
SfoI	GGCGCC	1 cut(s)	439
SinI	GGWCC	4 cut(s)	54, 177, 450, 2057
SmiMI	CAYNNNNRTG	3 cut(s)	1031, 2613, 2670
SmlI	CTYRAG	2 cut(s)	641, 1503
SmoI	CTYRAG	2 cut(s)	641, 1503
SspDI	GGCGCC	1 cut(s)	437
SspMI	CTAG	6 cut(s)	687, 1382, 1551, 1910, 2999, 3036
SstI	GAGCTC	1 cut(s)	705
StyD4I	CCNGG	9 cut(s)	161, 170, 360, 492, 534, 713, 1258, 1816, 2004
StyI	CCWWGG	7 cut(s)	39, 1519, 1579, 2017, 2472, 2754, 2898
TaaI	ACNGT	4 cut(s)	28, 1389, 2279, 2343
TaiI	ACGT	5 cut(s)	324, 522, 1925, 2674, 2779
TatI	WGTACW	1 cut(s)	2338
TauI	GCSGC	2 cut(s)	170, 360
TfiI	GAWTC	9 cut(s)	631, 694, 754, 923, 1244, 1454, 1880, 2093, 2972
Tru1I	TTAA	9 cut(s)	642, 1056, 1194, 1349, 1899, 2082, 2378, 2922, 3040
Tru9I	TTAA	9 cut(s)	642, 1056, 1194, 1349, 1899, 2082, 2378, 2922, 3040
TseFI	GTSAC	5 cut(s)	28, 829, 1160, 1296, 1439
Tsp45I	GTSAC	5 cut(s)	28, 829, 1160, 1296, 1439
TspGWI	ACGGA	4 cut(s)	37, 181, 2042, 2126
Vha464I	CTTAAG	1 cut(s)	641
VpaK11BI	GGWCC	4 cut(s)	54, 177, 450, 2057
XagI	CCTNNNNNAGG	1 cut(s)	539
XapI	RAATTY	9 cut(s)	91, 950, 1949, 2179, 2373, 2381, 2526, 2853, 3005
XbaI	TCTAGA	1 cut(s)	1909
XceI	RCATGY	2 cut(s)	1677, 2403
XcmI	CCANNNNNNNNNTGG	1 cut(s)	658
XmiI	GTMKAC	3 cut(s)	678, 877, 2135
XmnI	GAANNNNTTC	1 cut(s)	2355
XspI	CTAG	6 cut(s)	687, 1382, 1551, 1910, 2999, 3036
ZrmI	AGTACT	1 cut(s)	2340
Zsp2I	ATGCAT	1 cut(s)	2942

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.