Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0005460

Physical Location & Seq

Genomic Coordinates Reverse (-)

22429316 .. 22436306

Gene Structure 6991 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g21100.9

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2409 bp

ATGGATGTCGATTTGGTCGAGTGCGCAACGCAGATTCATGATTTGAATGAAACCAGGAGCAATTTGTTGGCTCTGCAGCAGAAATTGAGGCTCATTTTATATGTTAATCAAGCTCTTTCGGCACTTAAACTGCTTGTTGCCTCTGCAGATTGTGCTGGAGCCTTGGACGTCACAGACGATTTACAGCATTTGCTGGATGGAGACGAGCTTACTGGTCTACATTGCTTCCATCACCTTAGGGATCGCGTAGCGGCTTCAATTGAATCCATAAACAGCATTCTTTCTGCAGAGTTTATGCGTGCTTCAATACACGATGCTGGAGATACAGACGTTATAATTATATCCAGAGCAAAAGCAAGGGCATCTAGTCTGATGAATGGAGAAGATGGTGAAATTAAGTTGGATGATGAAGAAACCTCCAATTTTCAAGATCGTCTTCTTCCTGTCATTATTGGGTTGCTTAGAACAGCCAAGCTCCCTTCTGTGTTGAGGTTATATCGCGACCAACTTACAGCTGACATGAAGTCTGCTATCAAAAATGCCGTTGCAGAGCTGCTTCCCATTCTTGTTTCGCGACCACTGGAGTCAGATTTTACACCAGGGGAGCGAATAGTTGATGCAGATGGTTTTGGTGCATCACTTGCGAGCAAGTTGAGGAGCCTGTCATCTGAAAGCTTTGTTCAACTTTTAAGTGCTATTTTTCTGATTGTAAGGGCACATCTAGTGCGGGCTGCTGAAGTGAAGAAGGCCATTGAATGGATTATGTGCAACCTCGACGGTCACTATGCAGCCGATTCTGTTGCTGCAGCAATTGCAGTTGGTGCCGCAGCTGCAGAAACAGCTCAAGAGAGTGATGGTCAAGGTGGCTTGCTTATGCCATATTCACCTCAGAGGGTTGCTACAAAGGCCCTTTCAATTCAAGGGAAGGCAAATGATGCAGCAAGTCCTTCAAACATCTCTAAGAATTTTAGAGCTGATGTATTACGAGAAAACACAGAAGCTGTTGTTGCAGCATGTGATGCTGCTCATGGAAGATGGGCAAAGCTACTCGGGGTTCGTGCTCTTCTTCATCCTAAGTTGAGATTGCAGGAGTTTTTGAGCATATATAACATCACACAAAACTTTATAACAGCTACGGAGAAGATAGGGGGAATGCCAGGATTTAGCATCAGGGGAACACTACAGTCGCAGGCCAAAGCCTTCATGGATTTTCAGCATGAATCTCGAATGGCAAAAATTAAAGCTGTTCTTGACCAAGAAACGTGGGTGGAAGTGGATGTTCCTGATGAATTTCAGGTCATTGTCACTTCACTATTTAGTTCTGAATCATTGGTAACCGAGAACCTGGATACTGTCCAAGATAATACGGAAACTAGCTACAATGAGGTGGCCACACCCAGTAATAGTTCACATGCAGCAGATACAGGACCATCAATTGCTGAACAGCAATCTAAAGGGGCGGATTCTAGTGAAACATCTGCAGGTATTACTGCAAAGGAGACACCAAATTCTGATGGAACTGAGAAAAATAAGGCTGATGTTGCAAACTCTGTTGCTCAAAATAACCACAGCAACGTGAAGGAGCGAGGAAAATCAACCTCTCAAACCCTTTTCTACAAAGGTGTTGGTTTTCACATGGTAAACTGTGGCTTGATATTGATGAAGATGTTGTCAGAGTACATTGATATGAACAACTTTTTTCCAGCACTGTCCTCTGAAGTTGTTCATCGTATTGTAGAAATTTTAAAATTTTTCAATACAAGAACGTGTCAGCTTGTTCTTGGTGCCGGTGCCATGCAGGTATCCGGTTTAAAGTCTATCACTTCTAAACACTTGGCCTTGGCAAGTCAAGTCATCAGTTTTACATATGCTATTATTCCTGAAATCAGGCAAATTCTTTTTCTAAAAGTGCCAGAGACAAGAAAAGCATTGTTGTTATCTGAAATTGATCGAGTAGCTCAGGATTATAAGGTTCATAGAGATGAAATACACACCAAACTAGTTCAGATCATGAGAGAAAGGTTATTGGTTCATCTACGCGGGTTGCCTCAAATTGTAGAGAGCTGGAATAGACCTGAAGAGGCTGACCCACAGCCCAGCCAATTTGCTCGATCCCTTACCAAGGAAGTTGGATACTTGCAACGTGTTCTAACTCGAACTTTACATGAGGTCGATGTTCAAGCGATTTTCAGGCAAGTGATTTTGGTCTTCCATTCACAAATATCAGAGGCATTTTCACGGTTAGAGATCAGCACACCACAAGCCAAGGACAGGCTGCGCCGGGATGTCACACACATTCTTGGATGCATTCGATCTTTGCCCTCTGATAAAATGAGCGAATCTAGTATTCCAAACTGGGGTCAGCTAGATGAATTCTTGGTGCAGAGATTTGGCGCGGAAGCTGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000149 GO:0000938 GO:0001894 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005802 GO:0006810 GO:0006892 GO:0006896 GO:0006996 GO:0007010 GO:0007034 GO:0007041 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016482 GO:0019905 GO:0031090 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032501 GO:0032588 GO:0032991 GO:0040008 GO:0042147 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045103 GO:0045104 GO:0046907 GO:0048193 GO:0048471 GO:0048871 GO:0048872 GO:0048873 GO:0050789 GO:0050879 GO:0050881 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060052 GO:0060249 GO:0065007 GO:0065008 GO:0070013 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps54

Protein Analysis

803

Amino Acids

88.19

Weight (kDa)

5.71

Isoelectric Point (pI)

39.16

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005460)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19490 AT4G19490
fragaria_vesca	FvH4_6g01010 FvH4_6g01010 FvH4_6g01010
malus_domestica	MD04G1237800.v1.1 MD12G1256000.v1.1
prunus_persica	Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1
pyrus_communis	pycom04g21100 pycom12g23480
rosa_chinensis	RchiOBHm_Chr3g0448451
rosa_laevigata	RLG00000000687 RLG00000025905
rosa_multiflora	Rmu_sc0003308.1_g000012 Rmu_sc0011979.1_g000005
rosa_roxburghii	Rroxscaffold_6G00426680
rosa_rugosa	Rorug02G0612500 Rorug02G0612600 Rorug02G0612600 Rorug07G0334800
rosa_samantha	Rh3AG012000 Rh3BG011800 Rh3CG011500 Rh3DG012200 Rh7BG461900
rosa_wichuraiana	Rw3G000930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	335, 1127, 1968
AatII	GACGTC	1 cut(s)	171
Acc16I	TGCGCA	1 cut(s)	25
Acc36I	ACCTGC	2 cut(s)	1472, 1789
AccB1I	GGYRCC	3 cut(s)	821, 1784, 1790
AccB7I	CCANNNNNTGG	1 cut(s)	756
AccI	GTMKAC	1 cut(s)	217
AccII	CGCG	5 cut(s)	246, 501, 574, 2040, 2396
AciI	CCGC	6 cut(s)	251, 727, 825, 1460, 2040, 2396
AclWI	GGATC	2 cut(s)	249, 2106
AcoI	YGGCCR	1 cut(s)	1389
AcsI	RAATTY	7 cut(s)	964, 1289, 1507, 1740, 1748, 1893, 2372
AcuI	CTGAAG	3 cut(s)	756, 1737, 2097
AcyI	GRCGYC	1 cut(s)	168
AfaI	GTAC	1 cut(s)	1679
AfiI	CCNNNNNNNGG	4 cut(s)	756, 2122, 2271, 2357
AflIII	ACRYGT	2 cut(s)	1766, 2143
AhdI	GACNNNNNGTC	1 cut(s)	523
AhlI	ACTAGT	1 cut(s)	1999
AjnI	CCWGG	4 cut(s)	53, 598, 1156, 1344
Alw21I	GWGCWC	1 cut(s)	1063
Alw26I	GTCTC	3 cut(s)	195, 1493, 1910
AlwI	GGATC	2 cut(s)	249, 2106
AlwNI	CAGNNNCTG	1 cut(s)	1001
Ama87I	CYCGRG	1 cut(s)	1049
AoxI	GGCC	5 cut(s)	747, 906, 1191, 1389, 1836
ApoI	RAATTY	7 cut(s)	964, 1289, 1507, 1740, 1748, 1893, 2372
Asp700I	GAANNNNTTC	1 cut(s)	1722
AspLEI	GCGC	3 cut(s)	26, 2280, 2396
AspS9I	GGNCC	2 cut(s)	907, 1427
AsuC2I	CCSGG	1 cut(s)	2282
AsuHPI	GGTGA	3 cut(s)	224, 401, 876
AvaI	CYCGRG	1 cut(s)	1049
AvaII	GGWCC	1 cut(s)	1427
AxyI	CCTNAGG	1 cut(s)	236
BaeGI	GKGCMC	1 cut(s)	718
BaeI	ACNNNNGTAYC	2 cut(s)	315, 348
BalI	TGGCCA	1 cut(s)	1391
BanI	GGYRCC	3 cut(s)	821, 1784, 1790
BbsI	GAAGAC	2 cut(s)	428, 2200
Bbv12I	GWGCWC	1 cut(s)	1063
BccI	CCATC	8 cut(s)	191, 237, 380, 617, 848, 1029, 1438, 1508
BceAI	ACGGC	1 cut(s)	527
BcgI	CGANNNNNNTGC	4 cut(s)	782, 816, 1205, 1239
BciT130I	CCWGG	4 cut(s)	55, 600, 1158, 1346
BciVI	GTATCC	3 cut(s)	1342, 1813, 2126
BcnI	CCSGG	1 cut(s)	2282
BcoDI	GTCTC	3 cut(s)	195, 1493, 1910
BcuI	ACTAGT	1 cut(s)	1999
BfaI	CTAG	7 cut(s)	366, 722, 1374, 1467, 2000, 2343, 2366
BfmI	CTRYAG	7 cut(s)	74, 144, 285, 804, 831, 1181, 1479
BfuAI	ACCTGC	2 cut(s)	1472, 1789
BfuI	GTATCC	3 cut(s)	1342, 1813, 2126
Bme1390I	CCNGG	5 cut(s)	55, 600, 1158, 1346, 2282
Bme18I	GGWCC	1 cut(s)	1427
BmeRI	GACNNNNNGTC	1 cut(s)	523
BmeT110I	CYCGRG	1 cut(s)	1049
BmgT120I	GGNCC	2 cut(s)	907, 1427
BmiI	GGNNCC	5 cut(s)	160, 659, 823, 1786, 1792
BmrFI	CCNGG	5 cut(s)	55, 600, 1158, 1346, 2282
BmrI	ACTGGG	2 cut(s)	1392, 2365
BmsI	GCATC	8 cut(s)	304, 371, 607, 644, 925, 1009, 1176, 2294
BmuI	ACTGGG	2 cut(s)	1392, 2365
BpiI	GAAGAC	2 cut(s)	428, 2200
BpmI	CTGGAG	3 cut(s)	177, 339, 602
Bpu10I	CCTNAGC	1 cut(s)	1959
BpuEI	CTTGAG	1 cut(s)	828
BpuMI	CCSGG	1 cut(s)	2282
BsaHI	GRCGYC	1 cut(s)	168
BsaJI	CCNNGG	5 cut(s)	162, 599, 1839, 2121, 2265
BsaWI	WCCGGW	1 cut(s)	1805
Bsc4I	CCNNNNNNNGG	4 cut(s)	756, 2122, 2271, 2357
Bse118I	RCCGGY	1 cut(s)	1787
Bse1I	ACTGG	4 cut(s)	217, 585, 1398, 2360
Bse21I	CCTNAGG	1 cut(s)	236
Bse3DI	GCAATG	1 cut(s)	220
BseBI	CCWGG	4 cut(s)	55, 600, 1158, 1346
BseDI	CCNNGG	5 cut(s)	162, 599, 1839, 2121, 2265
BseGI	GGATG	7 cut(s)	10, 202, 409, 1069, 1282, 2290, 2309
BseLI	CCNNNNNNNGG	4 cut(s)	756, 2122, 2271, 2357
BseMI	GCAATG	1 cut(s)	220
BseMII	CTCAG	3 cut(s)	902, 1512, 1973
BseNI	ACTGG	4 cut(s)	217, 585, 1398, 2360
BseRI	GAGGAG	1 cut(s)	670
BseSI	GKGCMC	1 cut(s)	718
BseYI	CCCAGC	1 cut(s)	2096
BsgI	GTGCAG	1 cut(s)	2402
Bsh1236I	CGCG	5 cut(s)	246, 501, 574, 2040, 2396
BshFI	GGCC	5 cut(s)	749, 908, 1193, 1391, 1838
BshNI	GGYRCC	3 cut(s)	821, 1784, 1790
BsiHKAI	GWGCWC	1 cut(s)	1063
BsiHKCI	CYCGRG	1 cut(s)	1049
BsiSI	CCGG	3 cut(s)	1788, 1806, 2281
BslI	CCNNNNNNNGG	4 cut(s)	756, 2122, 2271, 2357
BsmAI	GTCTC	3 cut(s)	195, 1493, 1910
BsmBI	CGTCTC	1 cut(s)	195
BsmI	GAATGC	3 cut(s)	276, 1158, 2307
BsnI	GGCC	5 cut(s)	749, 908, 1193, 1391, 1838
BsoBI	CYCGRG	1 cut(s)	1049
Bsp1286I	GDGCHC	2 cut(s)	718, 1063
Bsp143I	GATC	7 cut(s)	241, 430, 1948, 2007, 2111, 2247, 2312
Bsp68I	TCGCGA	2 cut(s)	501, 574
BspACI	CCGC	6 cut(s)	251, 727, 825, 1460, 2040, 2396
BspANI	GGCC	5 cut(s)	749, 908, 1193, 1391, 1838
BspCNI	CTCAG	3 cut(s)	901, 1513, 1972
BspFNI	CGCG	5 cut(s)	246, 501, 574, 2040, 2396
BspHI	TCATGA	2 cut(s)	37, 2010
BspLI	GGNNCC	5 cut(s)	160, 659, 823, 1786, 1792
BspMAI	CTGCAG	6 cut(s)	78, 148, 289, 808, 835, 1483
BspMI	ACCTGC	2 cut(s)	1472, 1789
BspPI	GGATC	2 cut(s)	249, 2106
BspQI	GCTCTTC	1 cut(s)	1068
BspT107I	GGYRCC	3 cut(s)	821, 1784, 1790
BsrDI	GCAATG	1 cut(s)	220
BsrFI	RCCGGY	1 cut(s)	1787
BsrI	ACTGG	4 cut(s)	217, 585, 1398, 2360
BssAI	RCCGGY	1 cut(s)	1787
BssECI	CCNNGG	5 cut(s)	162, 599, 1839, 2121, 2265
BssMI	GATC	7 cut(s)	241, 430, 1948, 2007, 2111, 2247, 2312
BssNI	GRCGYC	1 cut(s)	168
BssT1I	CCWWGG	4 cut(s)	162, 1839, 2121, 2265
Bst2UI	CCWGG	4 cut(s)	55, 600, 1158, 1346
Bst4CI	ACNGT	6 cut(s)	779, 1185, 1354, 1646, 1710, 2241
Bst6I	CTCTTC	2 cut(s)	1068, 2073
BstACI	GRCGYC	1 cut(s)	168
BstAPI	GCANNNNNTGC	6 cut(s)	152, 641, 812, 821, 935, 1019
BstC8I	GCNNGC	5 cut(s)	300, 646, 729, 869, 1191
BstDEI	CTNAG	7 cut(s)	236, 461, 888, 960, 1074, 1521, 1959
BstEII	GGTNACC	1 cut(s)	1333
BstENI	CCTNNNNNAGG	1 cut(s)	2120
BstF5I	GGATG	7 cut(s)	10, 202, 409, 1069, 1282, 2290, 2309
BstFNI	CGCG	5 cut(s)	246, 501, 574, 2040, 2396
BstHHI	GCGC	3 cut(s)	26, 2280, 2396
BstKTI	GATC	7 cut(s)	244, 433, 1951, 2010, 2114, 2250, 2315
BstMAI	GTCTC	3 cut(s)	195, 1493, 1910
BstMBI	GATC	7 cut(s)	241, 430, 1948, 2007, 2111, 2247, 2312
BstNI	CCWGG	4 cut(s)	55, 600, 1158, 1346
BstNSI	RCATGY	2 cut(s)	1017, 1415
BstPI	GGTNACC	1 cut(s)	1333
BstSCI	CCNGG	5 cut(s)	53, 598, 1156, 1344, 2280
BstSFI	CTRYAG	7 cut(s)	74, 144, 285, 804, 831, 1181, 1479
BstSLI	GKGCMC	1 cut(s)	718
BstUI	CGCG	5 cut(s)	246, 501, 574, 2040, 2396
BstV2I	GAAGAC	2 cut(s)	428, 2200
Bsu36I	CCTNAGG	1 cut(s)	236
BsuI	GTATCC	3 cut(s)	1342, 1813, 2126
BsuRI	GGCC	5 cut(s)	749, 908, 1193, 1391, 1838
BtsCI	GGATG	7 cut(s)	10, 202, 409, 1069, 1282, 2290, 2309
BtsIMutI	CAGTG	2 cut(s)	578, 1706
BtuMI	TCGCGA	2 cut(s)	501, 574
BveI	ACCTGC	2 cut(s)	1472, 1789
Cac8I	GCNNGC	5 cut(s)	300, 646, 729, 869, 1191
CaiI	CAGNNNCTG	1 cut(s)	1001
CciI	TCATGA	2 cut(s)	37, 2010
CfoI	GCGC	3 cut(s)	26, 2280, 2396
Cfr10I	RCCGGY	1 cut(s)	1787
Cfr13I	GGNCC	2 cut(s)	907, 1427
Csp6I	GTAC	1 cut(s)	1678
CspCI	CAANNNNNGTGG	2 cut(s)	1244, 1279
CviQI	GTAC	1 cut(s)	1678
DdeI	CTNAG	7 cut(s)	236, 461, 888, 960, 1074, 1521, 1959
DpnI	GATC	7 cut(s)	243, 432, 1950, 2009, 2113, 2249, 2314
DpnII	GATC	7 cut(s)	241, 430, 1948, 2007, 2111, 2247, 2312
DraI	TTTAAA	2 cut(s)	1746, 1812
DriI	GACNNNNNGTC	1 cut(s)	523
EaeI	YGGCCR	1 cut(s)	1389
Eam1104I	CTCTTC	2 cut(s)	1068, 2073
Eam1105I	GACNNNNNGTC	1 cut(s)	523
EarI	CTCTTC	2 cut(s)	1068, 2073
EciI	GGCGGA	1 cut(s)	1475
Eco130I	CCWWGG	4 cut(s)	162, 1839, 2121, 2265
Eco47I	GGWCC	1 cut(s)	1427
Eco57I	CTGAAG	3 cut(s)	756, 1737, 2097
Eco81I	CCTNAGG	1 cut(s)	236
Eco88I	CYCGRG	1 cut(s)	1049
Eco91I	GGTNACC	1 cut(s)	1333
EcoNI	CCTNNNNNAGG	1 cut(s)	2120
EcoO109I	RGGNCCY	1 cut(s)	907
EcoO65I	GGTNACC	1 cut(s)	1333
EcoRI	GAATTC	1 cut(s)	2372
EcoRII	CCWGG	4 cut(s)	53, 598, 1156, 1344
EcoT14I	CCWWGG	4 cut(s)	162, 1839, 2121, 2265
EcoT22I	ATGCAT	1 cut(s)	2309
ErhI	CCWWGG	4 cut(s)	162, 1839, 2121, 2265
Esp3I	CGTCTC	1 cut(s)	195
FalI	AAGNNNNNCTT	4 cut(s)	420, 452, 1233, 1265
FauI	CCCGC	2 cut(s)	720, 2033
FauNDI	CATATG	1 cut(s)	1867
FblI	GTMKAC	1 cut(s)	217
FokI	GGATG	7 cut(s)	17, 209, 416, 1056, 1289, 2297, 2316
FspBI	CTAG	7 cut(s)	366, 722, 1374, 1467, 2000, 2343, 2366
FspI	TGCGCA	1 cut(s)	25
GlaI	GCGC	3 cut(s)	25, 2279, 2395
GsaI	CCCAGC	1 cut(s)	2100
GsuI	CTGGAG	3 cut(s)	177, 339, 602
HaeIII	GGCC	5 cut(s)	749, 908, 1193, 1391, 1838
HapII	CCGG	3 cut(s)	1788, 1806, 2281
HhaI	GCGC	3 cut(s)	26, 2280, 2396
Hin1I	GRCGYC	1 cut(s)	168
Hin6I	GCGC	3 cut(s)	24, 2278, 2394
HinP1I	GCGC	3 cut(s)	24, 2278, 2394
HindIII	AAGCTT	1 cut(s)	673
HinfI	GANTC	8 cut(s)	34, 263, 584, 794, 1220, 1325, 1463, 2339
HpaII	CCGG	3 cut(s)	1788, 1806, 2281
HphI	GGTGA	3 cut(s)	224, 401, 876
Hpy166II	GTNNAC	3 cut(s)	218, 1409, 1642
Hpy8I	GTNNAC	3 cut(s)	218, 1409, 1642
Hpy99I	CGWCG	1 cut(s)	779
HpyAV	CCTTC	6 cut(s)	489, 739, 919, 957, 1210, 1573
HpyCH4III	ACNGT	6 cut(s)	779, 1185, 1354, 1646, 1710, 2241
HpyCH4IV	ACGT	6 cut(s)	168, 330, 1262, 1575, 1766, 2143
HpyF3I	CTNAG	7 cut(s)	236, 461, 888, 960, 1074, 1521, 1959
HpySE526I	ACGT	6 cut(s)	168, 330, 1262, 1575, 1766, 2143
Hsp92I	GRCGYC	1 cut(s)	168
HspAI	GCGC	3 cut(s)	24, 2278, 2394
Kzo9I	GATC	7 cut(s)	241, 430, 1948, 2007, 2111, 2247, 2312
LguI	GCTCTTC	1 cut(s)	1068
LmnI	GCTCC	6 cut(s)	57, 158, 480, 604, 657, 1582
LweI	GCATC	8 cut(s)	304, 371, 607, 644, 925, 1009, 1176, 2294
MaeI	CTAG	7 cut(s)	366, 722, 1374, 1467, 2000, 2343, 2366
MaeII	ACGT	6 cut(s)	168, 330, 1262, 1575, 1766, 2143
MaeIII	GTNAC	5 cut(s)	169, 779, 1303, 1333, 2287
MalI	GATC	7 cut(s)	243, 432, 1950, 2009, 2113, 2249, 2314
MboI	GATC	7 cut(s)	241, 430, 1948, 2007, 2111, 2247, 2312
MfeI	CAATTG	3 cut(s)	258, 810, 1434
MhlI	GDGCHC	2 cut(s)	718, 1063
MlsI	TGGCCA	1 cut(s)	1391
MluNI	TGGCCA	1 cut(s)	1391
MlyI	GAGTC	1 cut(s)	593
MmeI	TCCRAC	2 cut(s)	381, 2110
Mox20I	TGGCCA	1 cut(s)	1391
Mph1103I	ATGCAT	1 cut(s)	2309
MroXI	GAANNNNTTC	1 cut(s)	1722
MscI	TGGCCA	1 cut(s)	1391
MseI	TTAA	8 cut(s)	105, 126, 396, 689, 1239, 1745, 1811, 2407
MslI	CAYNNNNRTG	2 cut(s)	1685, 1980
Msp20I	TGGCCA	1 cut(s)	1391
MspA1I	CMGCKG	2 cut(s)	515, 830
MspI	CCGG	3 cut(s)	1788, 1806, 2281
MspR9I	CCNGG	5 cut(s)	55, 600, 1158, 1346, 2282
MunI	CAATTG	3 cut(s)	258, 810, 1434
Mva1269I	GAATGC	3 cut(s)	276, 1158, 2307
MvaI	CCWGG	4 cut(s)	55, 600, 1158, 1346
MvnI	CGCG	5 cut(s)	246, 501, 574, 2040, 2396
NciI	CCSGG	1 cut(s)	2282
NdeI	CATATG	1 cut(s)	1867
NdeII	GATC	7 cut(s)	241, 430, 1948, 2007, 2111, 2247, 2312
NlaIV	GGNNCC	5 cut(s)	160, 659, 823, 1786, 1792
NmuCI	GTSAC	4 cut(s)	169, 779, 1303, 2287
NruI	TCGCGA	2 cut(s)	501, 574
NsbI	TGCGCA	1 cut(s)	25
NsiI	ATGCAT	1 cut(s)	2309
NspI	RCATGY	2 cut(s)	1017, 1415
PagI	TCATGA	2 cut(s)	37, 2010
PciSI	GCTCTTC	1 cut(s)	1068
PcsI	WCGNNNNNNNCGW	2 cut(s)	15, 174
PctI	GAATGC	3 cut(s)	276, 1158, 2307
PdmI	GAANNNNTTC	1 cut(s)	1722
PfeI	GAWTC	7 cut(s)	34, 263, 794, 1220, 1325, 1463, 2339
PflMI	CCANNNNNTGG	1 cut(s)	756
PleI	GAGTC	1 cut(s)	592
PpsI	GAGTC	1 cut(s)	592
PsiI	TTATAA	3 cut(s)	335, 1127, 1968
Psp6I	CCWGG	4 cut(s)	53, 598, 1156, 1344
PspEI	GGTNACC	1 cut(s)	1333
PspFI	CCCAGC	1 cut(s)	2096
PspGI	CCWGG	4 cut(s)	53, 598, 1156, 1344
PspN4I	GGNNCC	5 cut(s)	160, 659, 823, 1786, 1792
PspPI	GGNCC	2 cut(s)	907, 1427
PstI	CTGCAG	6 cut(s)	78, 148, 289, 808, 835, 1483
PstNI	CAGNNNCTG	1 cut(s)	1001
PvuII	CAGCTG	2 cut(s)	515, 830
RruI	TCGCGA	2 cut(s)	501, 574
RsaI	GTAC	1 cut(s)	1679
RsaNI	GTAC	1 cut(s)	1678
RseI	CAYNNNNRTG	2 cut(s)	1685, 1980
SapI	GCTCTTC	1 cut(s)	1068
SaqAI	TTAA	8 cut(s)	105, 126, 396, 689, 1239, 1745, 1811, 2407
Sau3AI	GATC	7 cut(s)	241, 430, 1948, 2007, 2111, 2247, 2312
Sau96I	GGNCC	2 cut(s)	907, 1427
SchI	GAGTC	1 cut(s)	593
ScrFI	CCNGG	5 cut(s)	55, 600, 1158, 1346, 2282
SduI	GDGCHC	2 cut(s)	718, 1063
SfaNI	GCATC	8 cut(s)	304, 371, 607, 644, 925, 1009, 1176, 2294
SfcI	CTRYAG	7 cut(s)	74, 144, 285, 804, 831, 1181, 1479
SinI	GGWCC	1 cut(s)	1427
SmiMI	CAYNNNNRTG	2 cut(s)	1685, 1980
SmlI	CTYRAG	1 cut(s)	843
SmoI	CTYRAG	1 cut(s)	843
SpeI	ACTAGT	1 cut(s)	1999
SsiI	CCGC	6 cut(s)	251, 727, 825, 1460, 2040, 2396
SspMI	CTAG	7 cut(s)	366, 722, 1374, 1467, 2000, 2343, 2366
StyD4I	CCNGG	5 cut(s)	53, 598, 1156, 1344, 2280
StyI	CCWWGG	4 cut(s)	162, 1839, 2121, 2265
TaaI	ACNGT	6 cut(s)	779, 1185, 1354, 1646, 1710, 2241
TaiI	ACGT	6 cut(s)	171, 333, 1265, 1578, 1769, 2146
TaqI	TCGA	9 cut(s)	9, 18, 774, 1225, 1951, 2110, 2155, 2172, 2311
TatI	WGTACW	1 cut(s)	1677
TauI	GCSGC	2 cut(s)	254, 827
TfiI	GAWTC	7 cut(s)	34, 263, 794, 1220, 1325, 1463, 2339
Tru1I	TTAA	8 cut(s)	105, 126, 396, 689, 1239, 1745, 1811, 2407
Tru9I	TTAA	8 cut(s)	105, 126, 396, 689, 1239, 1745, 1811, 2407
TscAI	CASTG	2 cut(s)	585, 1713
TseFI	GTSAC	4 cut(s)	169, 779, 1303, 2287
Tsp45I	GTSAC	4 cut(s)	169, 779, 1303, 2287
TspGWI	ACGGA	2 cut(s)	1151, 1382
TspRI	CASTG	2 cut(s)	585, 1713
Van91I	CCANNNNNTGG	1 cut(s)	756
VpaK11BI	GGWCC	1 cut(s)	1427
XagI	CCTNNNNNAGG	1 cut(s)	2120
XapI	RAATTY	7 cut(s)	964, 1289, 1507, 1740, 1748, 1893, 2372
XceI	RCATGY	2 cut(s)	1017, 1415
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1201
XmiI	GTMKAC	1 cut(s)	217
XmnI	GAANNNNTTC	1 cut(s)	1722
XspI	CTAG	7 cut(s)	366, 722, 1374, 1467, 2000, 2343, 2366
ZraI	GACGTC	1 cut(s)	169
Zsp2I	ATGCAT	1 cut(s)	2309

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.