Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0005460

Physical Location & Seq

Genomic Coordinates Forward (+)

47376051 .. 47385040

Gene Structure 8990 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00426680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGAGCAGCAATGGTTCTAAGAGAACGGTGACGACGACCATGGACTCCGTGAGGTCCTCCCACACCCGATCCAACTCCATATCCGACCCGAATTCCAGCACCAGCACCAGCCAGAGCCTCGCCTCCATCCTCAACAACCCCAATGCCTCCGACTCCTCCTCCTGGGCCGCCTGGTGGTCCTCCTCCGCCTCCGTCGCCCCGCCCGAGTTCCTCCCCCTCCTCCCCAAATCCGCATCAGACTCCCTGACCCGATCCGACTTCCTCCCCTACATCTCCTCCATTTCCGATCACTACAACCGCTTCGAGGACATCCTCAACCACGTCAAGAAGGAGAGCCTCGACTTCGACTCCATCGGCGGCCAGGGCGAAGCTCTCGTCGCTTGTCTCCGCGAAGTCCCCGCCCTCTATTTCAAAGAGGACTTCGCATTGGAAGACGGCGCCACCTTCCGCTCCGCCTGCCCCTTCTCCGGCGTCTCCGAGAACCTCGTCCTCCAGGAGAAGCTCTCGCATTACTTGGACGTGGTCGAGCTTCACCTGGTGAAGGAGATCTCGCTCCGATCAAACTCCTTCTTCGAGGCCCAGGGCCAGTTGCAGGACTTGAATGTGAAGATCGTTGAGGGATGCAATCGGATTCGGGAGCTTAAGGAGACCATTTTGCTCTTGGATGTGGATTTGGTCGACTCTGCTAGGCAGATTCAGGAGCTCAATGTTACCAGGAGTAATTTGTTGGCTCTTCAACAGAAACTCAGGCTAATTCTCTATGTCAATCAGGCTCTTTCGGCGCTCAAACTGCTTGTTGCCTCTGCAGATTGTGCTGGAGCTTTGGATGTCACAGATGACCTGCAGCATTTGCTGGACGGGGATGAACTTACTGGTCTACATTGCTTTCATCACCTCAGGGATCGTGTAGCAGCGTCAATTGAATCCATAAACAGCATTCTTTCTGCAGAATTTATGCGTGCTTCAATACATGATGCTGGAGATACAGACGCTATAATTATATCTAAAGCGAAAGCAAGGGCATCCATTCTCATGAATGGGGAAGATGGTGAAGTTAAGTTGGACGATGAAGAAACCTCCAATTATCAAGATCGTCTTCTTCCCATCATCATTGGTTTGCTTAGAACAGCTAAGCTCCCTTCTGTGTTGAGGCTATATCGTGACCAACTTACGGCTGATATGAAGACTGCTATTAAGAATGCAGTTGCAGAGCTGCTTCCTATTCTTGTTTCCCGACCTCTGGAATCCGATTTTACACCTGGAGAGCGAGTTGCAGATGCAGACGGCATAGGTGCGTCACTTGCAAGCAAGTTGAGGAGCCTGTCATCTGAAAGCTTTGTTCAACTTTTAAGTGCTATTTTTCTGATTGTAAGGGCACATCTAGTACGGTCTGCTGAAGTGAAGAAGGCTATTGAGTGGATTATGTGCAACCTTGATGGTCACTATGCAGCTGATTCAGTAGCTGCTGCACTTGCAATTGGCGCTGAGGCTGCAGAGACAGCTCAAGAAAGTGATGGCCAAGGTGGCTTGCTCGTGTCGTATTCGTCTCCTAGAGTTGCTGCAAAGGCCCTTTCATTCCAAGGAAAAGCAAATGATGCAACAAGTCCTTCTACTACATCTAAGAACTTTAGAGCTGATGTGTTACGAGAAAACACAGAAGCCGTTGTTGCAGCATGTGATGCTGCTCAGGGACGATGGGCAAAGCTACTTGGGGTTCGTGCTCTTCTTCATCCGAAGTTGAGATTACAGGAGTTTTTGAGCATATATAATATCACACAAGAGTTTGTAACTGCTACAGAGAAGGTAGGGGGAAGGCCGGGGTTTAGCATCAGAGGAACACTACAATCACAGGCCAAAGCCTTCCTCGATTTTCAGCATGAATCACGAATGACCAAGATTAAGGCAGTTCTAGACCAAGAAACGTGGGTGGAAGTAGATGTTCCTGATGAATTTCAGGTTATTGTCAATTCATTATTTTGTTCTGAAGCATTGGTAGCCGAGAACCTGGATGCTGGACATGGTAGTACGAAAACAAGCTACAATGAGGTGGCCAGAAGCAACAATAGTTCACATGTGGTGGATACAGGACCATCAATTACAGAAAAGCAAATTAAGCGAACTGATTCAACTGAATTGTCTACGGATATGACTGGAAATGATAAGTCTACATCTGCTGATGGAGCTGAAAAGAATAAGGCTGATGTTACAAATTCTGTGGCTCAGCACAACCACAGCAACATGAAGGAGAGGGGAAAATCAACTTCCCAAATCTTATCGTACAAAGGTGTTGGTTTTCACATGGTAAACTGTGGCCTGATATTGATGAAGATGTTGTCAGAGTATATTGATATGAACAATTTTTTTCCAGTACTGTCCTCAGAAGTTGTTCATCGTATTGTTGAAATTTTAAAATTTTTCAATACAAGAACATGTCAGCTTGTTCTTGGTGCTGGTGCCATGCAGGTATCTGGTCTGAAGTCGATCACTTCTAAACACTTGGCCTTGGCAAGTCAAGTCATCAGTTTTACATATGCTATTATTCCTGAACTCAGGCAAATTCTTTTTCTGAAAGTACCTGAGACAAGAAAGGCAATGTTGTTATCTGAAATTGATCGAGTAGCTCAGGATTATAAGGTTCATAGAGATGAAATACATACAAAGCTGGTTCAGATCATGAGAGAAAGGTTGTTGGTTCATCTACGTGGGTTGCCTCAAATTGTGGAGAGCTGGAATAGACCAGAAGATGCTGACCTACAGCCCAGTCAATTTGCTCGATCACTTACCAAGGAAGTTGGATACTTGCAACGTGTTCTAACTCGAACTTTACATGAGGTCGATGTTCAAGCAATTTTCAGGCAAGTGATTATAATTTTTCATTCACAAATTTCAGAAGCATTTTCACGCTTAGAGATCAGCACACCACAAGCCAAGGACAGGCTCTGTCGTGATGTTAAGCACATTCTTGGATGCATTCGATCTTTGCCTTCTGATAAACTGAGTGAATCTGGTACACCAAACTGGGGTCAACTAGATGAATTCTTGGTGCAGAGATTTGGGTCAGAAGCTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000149 GO:0000938 GO:0001894 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005802 GO:0006810 GO:0006892 GO:0006896 GO:0006996 GO:0007010 GO:0007034 GO:0007041 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016482 GO:0019905 GO:0031090 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032501 GO:0032588 GO:0032991 GO:0040008 GO:0042147 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045103 GO:0045104 GO:0046907 GO:0048193 GO:0048471 GO:0048871 GO:0048872 GO:0048873 GO:0050789 GO:0050879 GO:0050881 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060052 GO:0060249 GO:0065007 GO:0065008 GO:0070013 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps54

Protein Analysis

1023

Amino Acids

112.67

Weight (kDa)

5.76

Isoelectric Point (pI)

40.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps54	PF07928	765 - 895	8.3e-47	Vps54-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005460)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19490 AT4G19490
fragaria_vesca	FvH4_6g01010 FvH4_6g01010 FvH4_6g01010
malus_domestica	MD04G1237800.v1.1 MD12G1256000.v1.1
prunus_persica	Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1
pyrus_communis	pycom04g21100 pycom12g23480
rosa_chinensis	RchiOBHm_Chr3g0448451
rosa_laevigata	RLG00000000687 RLG00000025905
rosa_multiflora	Rmu_sc0003308.1_g000012 Rmu_sc0011979.1_g000005
rosa_roxburghii	Rroxscaffold_6G00426680
rosa_rugosa	Rorug02G0612500 Rorug02G0612600 Rorug02G0612600 Rorug07G0334800
rosa_samantha	Rh3AG012000 Rh3BG011800 Rh3CG011500 Rh3DG012200 Rh7BG461900
rosa_wichuraiana	Rw3G000930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2631, 2867
Acc36I	ACCTGC	2 cut(s)	849, 2452
AccB1I	GGYRCC	2 cut(s)	437, 2453
AccBSI	CCGCTC	1 cut(s)	450
AccI	GTMKAC	4 cut(s)	678, 877, 2138, 2165
AccII	CGCG	1 cut(s)	390
AclWI	GGATC	3 cut(s)	63, 246, 909
AcoI	YGGCCR	3 cut(s)	358, 1516, 2049
AcsI	RAATTY	9 cut(s)	91, 950, 1949, 2209, 2403, 2411, 2556, 2883, 3035
AcuI	CTGAAG	3 cut(s)	1416, 2004, 2495
AcyI	GRCGYC	2 cut(s)	438, 471
AdeI	CACNNNGTG	1 cut(s)	538
AfaI	GTAC	6 cut(s)	1386, 2026, 2279, 2370, 2574, 3010
AflII	CTTAAG	1 cut(s)	641
AflIII	ACRYGT	3 cut(s)	2071, 2429, 2806
AjiI	CACGTC	2 cut(s)	322, 520
AjnI	CCWGG	9 cut(s)	161, 170, 360, 492, 534, 579, 713, 1258, 2004
Alw21I	GWGCWC	2 cut(s)	705, 1723
Alw26I	GTCTC	6 cut(s)	389, 478, 641, 1490, 1551, 2573
AlwI	GGATC	3 cut(s)	63, 246, 909
AlwNI	CAGNNNCTG	2 cut(s)	1463, 2940
Ama87I	CYCGRG	1 cut(s)	203
ApoI	RAATTY	9 cut(s)	91, 950, 1949, 2209, 2403, 2411, 2556, 2883, 3035
ArsI	GACNNNNNNTTYG	2 cut(s)	360, 392
Asp700I	GAANNNNTTC	2 cut(s)	2122, 2385
AspLEI	GCGC	3 cut(s)	440, 784, 1484
AspS9I	GGNCC	7 cut(s)	54, 165, 177, 577, 583, 1567, 2087
AsuC2I	CCSGG	1 cut(s)	1818
AsuHPI	GGTGA	5 cut(s)	40, 524, 550, 884, 1061
AvaI	CYCGRG	1 cut(s)	203
AvaII	GGWCC	3 cut(s)	54, 177, 2087
AxyI	CCTNAGG	1 cut(s)	896
BaeGI	GKGCMC	1 cut(s)	1378
BalI	TGGCCA	2 cut(s)	1518, 2051
BanI	GGYRCC	2 cut(s)	437, 2453
BanII	GRGCYC	1 cut(s)	705
BauI	CACGAG	1 cut(s)	1532
BbsI	GAAGAC	3 cut(s)	438, 1088, 1190
Bbv12I	GWGCWC	2 cut(s)	705, 1723
BbvCI	CCTCAGC	1 cut(s)	1485
BccI	CCATC	9 cut(s)	134, 359, 1040, 1112, 1430, 1508, 1689, 2098, 2171
BceAI	ACGGC	4 cut(s)	451, 1188, 1300, 1646
BciT130I	CCWGG	9 cut(s)	163, 172, 362, 494, 536, 581, 715, 1260, 2006
BciVI	GTATCC	2 cut(s)	2074, 2789
BcnI	CCSGG	1 cut(s)	1818
BcoDI	GTCTC	6 cut(s)	389, 478, 641, 1490, 1551, 2573
BfaI	CTAG	6 cut(s)	687, 1382, 1551, 1910, 3029, 3066
BfmI	CTRYAG	6 cut(s)	804, 842, 945, 1491, 1794, 2753
BfoI	RGCGCY	3 cut(s)	441, 785, 1485
BfrI	CTTAAG	1 cut(s)	641
BfuAI	ACCTGC	2 cut(s)	849, 2452
BfuI	GTATCC	2 cut(s)	2074, 2789
BglI	GCCNNNNNGGC	1 cut(s)	1524
BglII	AGATCT	1 cut(s)	546
BlpI	GCTNAGC	2 cut(s)	1131, 2220
BmcAI	AGTACT	1 cut(s)	2370
Bme18I	GGWCC	3 cut(s)	54, 177, 2087
BmeT110I	CYCGRG	1 cut(s)	203
BmgBI	CACGTC	2 cut(s)	322, 520
BmgT120I	GGNCC	7 cut(s)	54, 165, 177, 577, 583, 1567, 2087
BmiI	GGNNCC	3 cut(s)	439, 1319, 2455
BmrI	ACTGGG	2 cut(s)	2754, 3028
BmuI	ACTGGG	2 cut(s)	2754, 3028
BpiI	GAAGAC	3 cut(s)	438, 1088, 1190
BplI	GAGNNNNNCTC	2 cut(s)	488, 520
BpmI	CTGGAG	4 cut(s)	476, 837, 999, 1281
Bpu10I	CCTNAGC	3 cut(s)	1485, 1686, 2622
Bpu1102I	GCTNAGC	2 cut(s)	1131, 2220
BpuEI	CTTGAG	1 cut(s)	1488
BpuMI	CCSGG	1 cut(s)	1818
BsaAI	YACGTR	1 cut(s)	2702
BsaHI	GRCGYC	2 cut(s)	438, 471
BsaI	GGTCTC	1 cut(s)	641
BsaXI	ACNNNNNCTCC	8 cut(s)	143, 173, 260, 290, 332, 362, 1254, 1284
Bse1I	ACTGG	6 cut(s)	586, 877, 2155, 2366, 2760, 3023
Bse21I	CCTNAGG	1 cut(s)	896
Bse3DI	GCAATG	3 cut(s)	16, 880, 2598
BseBI	CCWGG	9 cut(s)	163, 172, 362, 494, 536, 581, 715, 1260, 2006
BseMI	GCAATG	3 cut(s)	16, 880, 2598
BseNI	ACTGG	6 cut(s)	586, 877, 2155, 2366, 2760, 3023
BseRI	GAGGAG	6 cut(s)	145, 148, 172, 209, 265, 1330
BseSI	GKGCMC	1 cut(s)	1378
BsgI	GTGCAG	2 cut(s)	1452, 3065
Bsh1236I	CGCG	1 cut(s)	390
BshNI	GGYRCC	2 cut(s)	437, 2453
BsiHKAI	GWGCWC	2 cut(s)	705, 1723
BsiHKCI	CYCGRG	1 cut(s)	203
BsiSI	CCGG	2 cut(s)	468, 1817
BslFI	GGGAC	2 cut(s)	380, 1704
BsmAI	GTCTC	6 cut(s)	389, 478, 641, 1490, 1551, 2573
BsmBI	CGTCTC	2 cut(s)	478, 1551
BsmFI	GGGAC	2 cut(s)	380, 1704
BsmI	GAATGC	3 cut(s)	936, 1204, 2970
Bso31I	GGTCTC	1 cut(s)	641
BsoBI	CYCGRG	1 cut(s)	203
Bsp1286I	GDGCHC	3 cut(s)	705, 1378, 1723
Bsp1720I	GCTNAGC	2 cut(s)	1131, 2220
Bsp19I	CCATGG	1 cut(s)	39
BspFNI	CGCG	1 cut(s)	390
BspHI	TCATGA	2 cut(s)	1032, 2673
BspLI	GGNNCC	3 cut(s)	439, 1319, 2455
BspMAI	CTGCAG	4 cut(s)	808, 846, 949, 1495
BspMI	ACCTGC	2 cut(s)	849, 2452
BspPI	GGATC	3 cut(s)	63, 246, 909
BspQI	GCTCTTC	2 cut(s)	738, 1728
BspT107I	GGYRCC	2 cut(s)	437, 2453
BspTI	CTTAAG	1 cut(s)	641
BspTNI	GGTCTC	1 cut(s)	641
BsrBI	CCGCTC	1 cut(s)	450
BsrDI	GCAATG	3 cut(s)	16, 880, 2598
BsrI	ACTGG	6 cut(s)	586, 877, 2155, 2366, 2760, 3023
BssNI	GRCGYC	2 cut(s)	438, 471
BssSI	CACGAG	1 cut(s)	1532
BssT1I	CCWWGG	6 cut(s)	39, 1519, 1579, 2502, 2784, 2928
Bst2BI	CACGAG	1 cut(s)	1532
Bst2UI	CCWGG	9 cut(s)	163, 172, 362, 494, 536, 581, 715, 1260, 2006
Bst4CI	ACNGT	4 cut(s)	28, 1389, 2309, 2373
Bst6I	CTCTTC	2 cut(s)	738, 1728
BstACI	GRCGYC	2 cut(s)	438, 471
BstAFI	CTTAAG	1 cut(s)	641
BstAPI	GCANNNNNTGC	4 cut(s)	812, 850, 1595, 1679
BstBAI	YACGTR	1 cut(s)	2702
BstC8I	GCNNGC	4 cut(s)	457, 960, 1306, 1529
BstDSI	CCRYGG	1 cut(s)	39
BstENI	CCTNNNNNAGG	1 cut(s)	539
BstFNI	CGCG	1 cut(s)	390
BstH2I	RGCGCY	3 cut(s)	441, 785, 1485
BstHHI	GCGC	3 cut(s)	440, 784, 1484
BstMAI	GTCTC	6 cut(s)	389, 478, 641, 1490, 1551, 2573
BstNI	CCWGG	9 cut(s)	163, 172, 362, 494, 536, 581, 715, 1260, 2006
BstNSI	RCATGY	3 cut(s)	1677, 2075, 2433
BstSFI	CTRYAG	6 cut(s)	804, 842, 945, 1491, 1794, 2753
BstSLI	GKGCMC	1 cut(s)	1378
BstUI	CGCG	1 cut(s)	390
BstV2I	GAAGAC	3 cut(s)	438, 1088, 1190
BstX2I	RGATCY	1 cut(s)	546
BstYI	RGATCY	1 cut(s)	546
Bsu36I	CCTNAGG	1 cut(s)	896
BsuI	GTATCC	2 cut(s)	2074, 2789
BtgI	CCRYGG	1 cut(s)	39
BtrI	CACGTC	2 cut(s)	322, 520
BveI	ACCTGC	2 cut(s)	849, 2452
Cac8I	GCNNGC	4 cut(s)	457, 960, 1306, 1529
CaiI	CAGNNNCTG	2 cut(s)	1463, 2940
CciI	TCATGA	2 cut(s)	1032, 2673
CfoI	GCGC	3 cut(s)	440, 784, 1484
Cfr13I	GGNCC	7 cut(s)	54, 165, 177, 577, 583, 1567, 2087
CseI	GACGC	4 cut(s)	460, 903, 998, 1284
CsiI	ACCWGGT	1 cut(s)	534
Csp6I	GTAC	6 cut(s)	1385, 2025, 2278, 2369, 2573, 3009
CspCI	CAANNNNNGTGG	4 cut(s)	1904, 1939, 2196, 2231
CviQI	GTAC	6 cut(s)	1385, 2025, 2278, 2369, 2573, 3009
DinI	GGCGCC	1 cut(s)	439
DraI	TTTAAA	1 cut(s)	2409
DraIII	CACNNNGTG	1 cut(s)	538
EaeI	YGGCCR	3 cut(s)	358, 1516, 2049
Eam1104I	CTCTTC	2 cut(s)	738, 1728
EarI	CTCTTC	2 cut(s)	738, 1728
EciI	GGCGGA	2 cut(s)	175, 442
Ecl136II	GAGCTC	1 cut(s)	703
Eco130I	CCWWGG	6 cut(s)	39, 1519, 1579, 2502, 2784, 2928
Eco24I	GRGCYC	1 cut(s)	705
Eco31I	GGTCTC	1 cut(s)	641
Eco47I	GGWCC	3 cut(s)	54, 177, 2087
Eco53kI	GAGCTC	1 cut(s)	703
Eco57I	CTGAAG	3 cut(s)	1416, 2004, 2495
Eco81I	CCTNAGG	1 cut(s)	896
Eco88I	CYCGRG	1 cut(s)	203
EcoICRI	GAGCTC	1 cut(s)	703
EcoNI	CCTNNNNNAGG	1 cut(s)	539
EcoO109I	RGGNCCY	2 cut(s)	54, 1567
EcoRI	GAATTC	2 cut(s)	91, 3035
EcoRII	CCWGG	9 cut(s)	161, 170, 360, 492, 534, 579, 713, 1258, 2004
EcoT14I	CCWWGG	6 cut(s)	39, 1519, 1579, 2502, 2784, 2928
EcoT22I	ATGCAT	1 cut(s)	2972
EcoT38I	GRGCYC	1 cut(s)	705
EgeI	GGCGCC	1 cut(s)	439
EheI	GGCGCC	1 cut(s)	439
ErhI	CCWWGG	6 cut(s)	39, 1519, 1579, 2502, 2784, 2928
Esp3I	CGTCTC	2 cut(s)	478, 1551
FalI	AAGNNNNNCTT	2 cut(s)	1080, 1112
FaqI	GGGAC	2 cut(s)	380, 1704
FauI	CCCGC	2 cut(s)	207, 406
FauNDI	CATATG	1 cut(s)	2530
FblI	GTMKAC	4 cut(s)	678, 877, 2138, 2165
FriOI	GRGCYC	1 cut(s)	705
FspBI	CTAG	6 cut(s)	687, 1382, 1551, 1910, 3029, 3066
GlaI	GCGC	3 cut(s)	439, 783, 1483
GsuI	CTGGAG	4 cut(s)	476, 837, 999, 1281
HaeII	RGCGCY	3 cut(s)	441, 785, 1485
HapII	CCGG	2 cut(s)	468, 1817
HgaI	GACGC	4 cut(s)	460, 903, 998, 1284
HhaI	GCGC	3 cut(s)	440, 784, 1484
Hin1I	GRCGYC	2 cut(s)	438, 471
Hin6I	GCGC	3 cut(s)	438, 782, 1482
HinP1I	GCGC	3 cut(s)	438, 782, 1482
HincII	GTYRAC	2 cut(s)	679, 3026
HindII	GTYRAC	2 cut(s)	679, 3026
HindIII	AAGCTT	1 cut(s)	1333
HpaII	CCGG	2 cut(s)	468, 1817
HphI	GGTGA	5 cut(s)	40, 524, 550, 884, 1061
Hpy166II	GTNNAC	8 cut(s)	679, 878, 2069, 2139, 2166, 2305, 3011, 3026
Hpy8I	GTNNAC	8 cut(s)	679, 878, 2069, 2139, 2166, 2305, 3011, 3026
Hpy99I	CGWCG	3 cut(s)	37, 197, 380
HpyCH4III	ACNGT	4 cut(s)	28, 1389, 2309, 2373
HpyCH4IV	ACGT	5 cut(s)	321, 519, 1922, 2701, 2806
HpySE526I	ACGT	5 cut(s)	321, 519, 1922, 2701, 2806
Hsp92I	GRCGYC	2 cut(s)	438, 471
HspAI	GCGC	3 cut(s)	438, 782, 1482
KasI	GGCGCC	1 cut(s)	437
LguI	GCTCTTC	2 cut(s)	738, 1728
LmnI	GCTCC	8 cut(s)	455, 558, 637, 700, 818, 1140, 1317, 2180
MabI	ACCWGGT	1 cut(s)	534
MaeI	CTAG	6 cut(s)	687, 1382, 1551, 1910, 3029, 3066
MaeII	ACGT	5 cut(s)	321, 519, 1922, 2701, 2806
MaeIII	GTNAC	9 cut(s)	28, 709, 829, 1160, 1296, 1439, 1641, 1786, 2203
MbiI	CCGCTC	1 cut(s)	450
MfeI	CAATTG	2 cut(s)	918, 1476
MflI	RGATCY	1 cut(s)	546
MhlI	GDGCHC	3 cut(s)	705, 1378, 1723
MlsI	TGGCCA	2 cut(s)	1518, 2051
MluNI	TGGCCA	2 cut(s)	1518, 2051
Mly113I	GGCGCC	1 cut(s)	438
MlyI	GAGTC	5 cut(s)	38, 146, 233, 341, 674
MmeI	TCCRAC	6 cut(s)	96, 108, 174, 279, 1041, 2773
Mox20I	TGGCCA	2 cut(s)	1518, 2051
Mph1103I	ATGCAT	1 cut(s)	2972
MroXI	GAANNNNTTC	2 cut(s)	2122, 2385
MscI	TGGCCA	2 cut(s)	1518, 2051
MseI	TTAA	9 cut(s)	642, 1056, 1194, 1349, 1899, 2112, 2408, 2952, 3070
MslI	CAYNNNNRTG	3 cut(s)	1031, 2643, 2700
Msp20I	TGGCCA	2 cut(s)	1518, 2051
MspA1I	CMGCKG	1 cut(s)	1451
MspCI	CTTAAG	1 cut(s)	641
MspI	CCGG	2 cut(s)	468, 1817
MunI	CAATTG	2 cut(s)	918, 1476
Mva1269I	GAATGC	3 cut(s)	936, 1204, 2970
MvaI	CCWGG	9 cut(s)	163, 172, 362, 494, 536, 581, 715, 1260, 2006
MvnI	CGCG	1 cut(s)	390
NarI	GGCGCC	1 cut(s)	438
NciI	CCSGG	1 cut(s)	1818
NcoI	CCATGG	1 cut(s)	39
NdeI	CATATG	1 cut(s)	2530
NlaIV	GGNNCC	3 cut(s)	439, 1319, 2455
NmeAIII	GCCGAG	1 cut(s)	2023
NmuCI	GTSAC	5 cut(s)	28, 829, 1160, 1296, 1439
NsiI	ATGCAT	1 cut(s)	2972
NspI	RCATGY	3 cut(s)	1677, 2075, 2433
PagI	TCATGA	2 cut(s)	1032, 2673
PasI	CCCWGGG	1 cut(s)	580
PciI	ACATGT	2 cut(s)	2071, 2429
PciSI	GCTCTTC	2 cut(s)	738, 1728
PcsI	WCGNNNNNNNCGW	2 cut(s)	32, 201
PctI	GAATGC	3 cut(s)	936, 1204, 2970
PdmI	GAANNNNTTC	2 cut(s)	2122, 2385
PfeI	GAWTC	8 cut(s)	631, 694, 923, 1244, 1454, 1880, 2123, 3002
PflFI	GACNNNGTC	1 cut(s)	521
PfoI	TCCNGGA	1 cut(s)	492
PleI	GAGTC	5 cut(s)	38, 146, 233, 341, 674
PluTI	GGCGCC	1 cut(s)	441
PpsI	GAGTC	5 cut(s)	38, 146, 233, 341, 674
Ppu21I	YACGTR	1 cut(s)	2702
PpuMI	RGGWCCY	1 cut(s)	54
PscI	ACATGT	2 cut(s)	2071, 2429
PsiI	TTATAA	2 cut(s)	2631, 2867
Psp124BI	GAGCTC	1 cut(s)	705
Psp5II	RGGWCCY	1 cut(s)	54
Psp6I	CCWGG	9 cut(s)	161, 170, 360, 492, 534, 579, 713, 1258, 2004
PspGI	CCWGG	9 cut(s)	161, 170, 360, 492, 534, 579, 713, 1258, 2004
PspN4I	GGNNCC	3 cut(s)	439, 1319, 2455
PspPI	GGNCC	7 cut(s)	54, 165, 177, 577, 583, 1567, 2087
PspPPI	RGGWCCY	1 cut(s)	54
PstI	CTGCAG	4 cut(s)	808, 846, 949, 1495
PstNI	CAGNNNCTG	2 cut(s)	1463, 2940
PsuI	RGATCY	1 cut(s)	546
PsyI	GACNNNGTC	1 cut(s)	521
PvuII	CAGCTG	1 cut(s)	1451
RsaI	GTAC	6 cut(s)	1386, 2026, 2279, 2370, 2574, 3010
RsaNI	GTAC	6 cut(s)	1385, 2025, 2278, 2369, 2573, 3009
RseI	CAYNNNNRTG	3 cut(s)	1031, 2643, 2700
SacI	GAGCTC	1 cut(s)	705
SalI	GTCGAC	1 cut(s)	677
SapI	GCTCTTC	2 cut(s)	738, 1728
SaqAI	TTAA	9 cut(s)	642, 1056, 1194, 1349, 1899, 2112, 2408, 2952, 3070
Sau96I	GGNCC	7 cut(s)	54, 165, 177, 577, 583, 1567, 2087
ScaI	AGTACT	1 cut(s)	2370
SchI	GAGTC	5 cut(s)	38, 146, 233, 341, 674
SduI	GDGCHC	3 cut(s)	705, 1378, 1723
SexAI	ACCWGGT	1 cut(s)	534
SfcI	CTRYAG	6 cut(s)	804, 842, 945, 1491, 1794, 2753
SfoI	GGCGCC	1 cut(s)	439
SinI	GGWCC	3 cut(s)	54, 177, 2087
SmiMI	CAYNNNNRTG	3 cut(s)	1031, 2643, 2700
SmlI	CTYRAG	2 cut(s)	641, 1503
SmoI	CTYRAG	2 cut(s)	641, 1503
SspDI	GGCGCC	1 cut(s)	437
SspMI	CTAG	6 cut(s)	687, 1382, 1551, 1910, 3029, 3066
SstI	GAGCTC	1 cut(s)	705
StyI	CCWWGG	6 cut(s)	39, 1519, 1579, 2502, 2784, 2928
TaaI	ACNGT	4 cut(s)	28, 1389, 2309, 2373
TaiI	ACGT	5 cut(s)	324, 522, 1925, 2704, 2809
TatI	WGTACW	1 cut(s)	2368
TauI	GCSGC	2 cut(s)	170, 360
TfiI	GAWTC	8 cut(s)	631, 694, 923, 1244, 1454, 1880, 2123, 3002
Tru1I	TTAA	9 cut(s)	642, 1056, 1194, 1349, 1899, 2112, 2408, 2952, 3070
Tru9I	TTAA	9 cut(s)	642, 1056, 1194, 1349, 1899, 2112, 2408, 2952, 3070
TseFI	GTSAC	5 cut(s)	28, 829, 1160, 1296, 1439
Tsp45I	GTSAC	5 cut(s)	28, 829, 1160, 1296, 1439
TspGWI	ACGGA	3 cut(s)	37, 181, 2156
Tth111I	GACNNNGTC	1 cut(s)	521
Vha464I	CTTAAG	1 cut(s)	641
VpaK11BI	GGWCC	3 cut(s)	54, 177, 2087
XagI	CCTNNNNNAGG	1 cut(s)	539
XapI	RAATTY	9 cut(s)	91, 950, 1949, 2209, 2403, 2411, 2556, 2883, 3035
XbaI	TCTAGA	1 cut(s)	1909
XceI	RCATGY	3 cut(s)	1677, 2075, 2433
XcmI	CCANNNNNNNNNTGG	1 cut(s)	658
XmiI	GTMKAC	4 cut(s)	678, 877, 2138, 2165
XmnI	GAANNNNTTC	2 cut(s)	2122, 2385
XspI	CTAG	6 cut(s)	687, 1382, 1551, 1910, 3029, 3066
ZrmI	AGTACT	1 cut(s)	2370
Zsp2I	ATGCAT	1 cut(s)	2972

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.