Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005460

Physical Location & Seq

Genomic Coordinates Reverse (-)

32369850 .. 32377739

Gene Structure 7890 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1256000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3030 bp

ATGGTAATCATGGACGCCCGACCCGGAAAGTTGAACAGCCGATCCAATTCCATATCCGACCCGAACACCAGCCAAAGCCTCGCCTCCATCCTCAACAACCCCAATGCCTCCGACGCCTCCTCCTGGGTCGGCTGGTGGTCCTCCTCCGCCTCCGTCTCCCCGCCCGAATTCGCTCCTCTCGTGCCTAAATCGGCATCGGACTCCGTGACCCGATCCGACTTCCAGCCTTACCTCGCCTCCGTCTCCGATCACTACAACCGCTTCGCGGACATCCTCAACCACGTCAAGAAGGAGAAATCCGACGTCGACTCCATCGGCGGCCAAGGAGAAGCCCTCGTCGCGTGCCTCCGGGAAGTCCCGGCGCTCTATTTCAAGGAGGACTTCGCCCTGGAAGACGGCGCCACGTTCCGTTCGGCGTGCCCGTTCACGAACGTCTCCGAGAACCTGGTGCTGCAGGAGAAGCTGTCGCATTACTTGGACGTGGTGGAGCTTCACTTGGTGAAGGAGATCTCGCTGCGTTCCAACTCCTTCTTTGAGGCCCAGGGGCAGTTGCAGGACTTGAACGTCAAGATCGTCGAGGGATGCAGTCGGATTCGAGAGCTCAAGGAGACAATTTTGCTCTTGGATGTCGATTTGGTCGACTGCGCAAGGCAGATTCATGATCTGAATGAAACCAGGAGCAATTTGTTGGCTCTGCAGCAGAAATTGAGGCTCATTTTATACGTCAATCAAGCTCTTTCGGCACTTAAACTCCTTGTTGCCTCTGCAGATTGTGCTGGAGCCTTGGACGTCACAGATGATTTACAGCATTTGCTGGATGGGGATGAGCTTACTGGTCTACATTGCTTTCATCACCTCCGGGATCGTGTAGCTGCTTCAATTGAATCGATAAATAGCATTCTTTCTGCAGAGTTTATGCGTGCTTCAATACACGATGCCGGAGATACAGATGTTATAATTATATCCAGAGTGAAAGCAAGGGCATCTAGTCTCATGAATGGAGAAGGAGAAGATGGTGAAATTAAGTTGGATGATGAAGAAACCTCCAATTTTCAAGATCGTCTTCTTCCTGTCATCATTGGATTGCTTAGAACTGCCAAGCTCCCTTCTGTGTTGAGGTTATATCGTGACCAACTTACAGCTGACATGAAGACTGCTATTAAAAATGCGGTTGCAGAGCTGCTTCCCATTCTTGTTTCACGACCACTGGAGTCAGATTTTACACCTGGAGAGCGAGTTGATGCAGATGGTTTTGGCGCATCACTTGCGAGCAAGTTGAGGAGCCTGTCATCTGAGAGCTTTATTCAACTTTTAAGTGCTATTTTTCTAATTGTAAGGGCACATCTAATGCGGGCTGCTGAAGTGAAGAAGGCCATTGAATGGATTATGTGCAACCTTGATGGTCACTATGCAGCCAATTCAGTTGCTGCAGCGATTGCAGTTGGTGCTGCGGCTGCAGAAACAGCTCAAGAGGGTGATGGTCAAGGTGGCTTGCTTATGCCATATTCACCCCATAGGGTTGCTACAAAGGCACTTTCAATTCAAGGGAAAGCTAATGATGCAACAAGTCCTTCAAACATCTCTAAGAATTTTAGAGCTGATGTGTTACGAGAAAACACAGAAGCTGTTGTTGCAGCATGTGATGCTGCTCATGGAAGATGGGCAAAACTACTCGGTGTTCGTGCTCTTCTTCATCCAAGGTTGAGATTGCAGGAGTTTTTGAGCATATATAACATCACCCAAGACTTTATAACAGCTACAGAGAAGATAGGGGGAAGGCCGGGATTTAGCATCAGGGGAACAATACAGTCACAGGCCAAAGCCTTTATGGATTTTCAGCATGAATCTCGTATGGCAAAAATTAAGGCAGTTCTTGACCAAGAAACTTGGGTGGAAGTGGATGTTCCTGATGAATTTCAGATCATTGTCACTTCACTATTTAGTTCTGAATCATTGGTTTCCCAGAACCTGGATGCTGTCCAAGATAATACGGAAACAAGTTACAATGAGGTGGCCACAAGCAGTAATAGTTCACATGCAGCAGAAACGGAATCATCAGTTGCTGAACAGCAATCTAAAGGGGCGGATTCTAGTGAAACATCTGCGGATGTTACTGTAAAGGAGACACCAAAGTCTGATGGAACTGAGAAAAATAAAGCTGATGTTGCAAATTCTGTTGCTCAAAATAACCATAGCAATAAGGAGCGAGGAAAATCAACCTCTCAAAGCCTTTTCTACAAAGGTGTTGGTTTTCACATGGTAAACTGTGGCTTGATATTGATGAAGATGTTGTCAGAATACATTGATATGAACAACTTTTTCCCAACACTGTCCTCAGAAGTTGTTCATCGTATTGTAGAAATTTTAAAATTTTTCAATACAAGAACGTGTCAGCTTGTTCTTGGTGCTGGTGCCATGCAGGTATCCGGTTTAAAGTCTATTACTTCTAAACACTTGGCCTTGGCAAGTCAAGTCATCAGTTTTACGTATGCTATTATTCCTGAAATCAGGCAAATTCTTTTTCAAAAAGTGCCAGAGACAAGAAAGGCATTGCTGCTATCAGAAATTGATCGAGTAGCTCAGGATTATAAGGTTCACAGAGATGAAATACACACCAAGCTAGTTCAAATCATGAGAGAAAGGTTATTGGTTCATCTACGCGGGTTGCCCCAAATTGTAGAGAGCTGGAATAGACCTGAAGAGGCTGATCCACAGCCTAGCCAGTTTGCTCGATCCCTTACCAAGGAAGTTGGATACTTACAACGTGTTTTAACTCGAACTTTACATGAGGTTGATGTCCAAGCGATTTTCAGGCAAGTAATTTTGGTCTTCCATTCACAAATATCAGAAGCATTTTCACGGTTAGAGATCAGCACACCACAAGCCAAGGACAGGCTGCGCCGGGATGTCAAACACATTCTTGGCTGCATTCGATGTTTACCTTCGGATAAAATGAGCGAATCTAGTATTCCAAACTGGGGTCAGCTAGATGAATTTTTGGTGCAGAGATTTGGCGAGGAAGCCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000149 GO:0000938 GO:0001894 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005802 GO:0006810 GO:0006892 GO:0006896 GO:0006996 GO:0007010 GO:0007034 GO:0007041 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016482 GO:0019905 GO:0031090 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032501 GO:0032588 GO:0032991 GO:0040008 GO:0042147 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045103 GO:0045104 GO:0046907 GO:0048193 GO:0048471 GO:0048871 GO:0048872 GO:0048873 GO:0050789 GO:0050879 GO:0050881 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060052 GO:0060249 GO:0065007 GO:0065008 GO:0070013 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps54

Protein Analysis

1010

Amino Acids

111.39

Weight (kDa)

5.61

Isoelectric Point (pI)

42.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps54	PF07928	751 - 881	5.6e-48	Vps54-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005460)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19490 AT4G19490
fragaria_vesca	FvH4_6g01010 FvH4_6g01010 FvH4_6g01010
malus_domestica	MD04G1237800.v1.1 MD12G1256000.v1.1
prunus_persica	Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1
pyrus_communis	pycom04g21100 pycom12g23480
rosa_chinensis	RchiOBHm_Chr3g0448451
rosa_laevigata	RLG00000000687 RLG00000025905
rosa_multiflora	Rmu_sc0003308.1_g000012 Rmu_sc0011979.1_g000005
rosa_roxburghii	Rroxscaffold_6G00426680
rosa_rugosa	Rorug02G0612500 Rorug02G0612600 Rorug02G0612600 Rorug07G0334800
rosa_samantha	Rh3AG012000 Rh3BG011800 Rh3CG011500 Rh3DG012200 Rh7BG461900
rosa_wichuraiana	Rw3G000930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	956, 1751, 2589
AasI	GACNNNNNNGTC	2 cut(s)	563, 2131
AatII	GACGTC	2 cut(s)	306, 792
Acc16I	TGCGCA	1 cut(s)	646
Acc36I	ACCTGC	1 cut(s)	2410
AccB1I	GGYRCC	2 cut(s)	398, 2411
AccB7I	CCANNNNNTGG	2 cut(s)	1380, 1969
AccI	GTMKAC	3 cut(s)	306, 639, 838
AccII	CGCG	3 cut(s)	266, 341, 2661
AclWI	GGATC	5 cut(s)	36, 207, 870, 2702, 2727
AcoI	YGGCCR	2 cut(s)	319, 2013
AcsI	RAATTY	8 cut(s)	167, 1588, 1913, 2170, 2361, 2369, 2514, 2993
AcuI	CTGAAG	2 cut(s)	1380, 2718
AcyI	GRCGYC	5 cut(s)	15, 114, 303, 399, 789
AdeI	CACNNNGTG	1 cut(s)	499
AfiI	CCNNNNNNNGG	7 cut(s)	123, 265, 1380, 1969, 2743, 2892, 2978
AflIII	ACRYGT	2 cut(s)	2387, 2764
AjiI	CACGTC	2 cut(s)	283, 481
AjnI	CCWGG	7 cut(s)	122, 387, 444, 540, 674, 1225, 1968
AloI	GAACNNNNNNTCC	2 cut(s)	26, 58
Alw21I	GWGCWC	2 cut(s)	603, 1687
Alw26I	GTCTC	7 cut(s)	160, 247, 439, 602, 995, 2117, 2531
AlwI	GGATC	5 cut(s)	36, 207, 870, 2702, 2727
AlwNI	CAGNNNCTG	4 cut(s)	1427, 1625, 1969, 2063
AoxI	GGCC	7 cut(s)	319, 537, 1371, 1778, 1815, 2013, 2457
ApoI	RAATTY	8 cut(s)	167, 1588, 1913, 2170, 2361, 2369, 2514, 2993
Asp700I	GAANNNNTTC	1 cut(s)	2343
AspLEI	GCGC	5 cut(s)	364, 401, 647, 1259, 2901
AspS9I	GGNCC	2 cut(s)	138, 538
AsuC2I	CCSGG	6 cut(s)	24, 350, 359, 860, 1782, 2903
AsuHPI	GGTGA	6 cut(s)	511, 845, 1028, 1487, 1500, 1729
AvaII	GGWCC	1 cut(s)	138
BaeGI	GKGCMC	2 cut(s)	422, 1342
BaeI	ACNNNNGTAYC	2 cut(s)	936, 969
BalI	TGGCCA	1 cut(s)	2015
BanI	GGYRCC	2 cut(s)	398, 2411
BanII	GRGCYC	1 cut(s)	603
BauI	CACGAG	1 cut(s)	179
BbsI	GAAGAC	4 cut(s)	399, 1055, 1157, 2821
Bbv12I	GWGCWC	2 cut(s)	603, 1687
BccI	CCATC	9 cut(s)	95, 320, 812, 1007, 1241, 1394, 1472, 1653, 2132
BceAI	ACGGC	1 cut(s)	412
BcgI	CGANNNNNNTGC	2 cut(s)	1829, 1863
BciT130I	CCWGG	7 cut(s)	124, 389, 446, 542, 676, 1227, 1970
BciVI	GTATCC	2 cut(s)	2434, 2747
BcnI	CCSGG	6 cut(s)	24, 350, 359, 860, 1782, 2903
BcoDI	GTCTC	7 cut(s)	160, 247, 439, 602, 995, 2117, 2531
BfaI	CTAG	6 cut(s)	987, 2091, 2621, 2718, 2964, 2987
BfmI	CTRYAG	7 cut(s)	452, 695, 765, 906, 1428, 1455, 1758
BfoI	RGCGCY	2 cut(s)	365, 402
BfuAI	ACCTGC	1 cut(s)	2410
BfuI	GTATCC	2 cut(s)	2434, 2747
BglII	AGATCT	1 cut(s)	507
Bme18I	GGWCC	1 cut(s)	138
BmgBI	CACGTC	2 cut(s)	283, 481
BmgT120I	GGNCC	2 cut(s)	138, 538
BmiI	GGNNCC	4 cut(s)	400, 781, 1283, 2413
BmrI	ACTGGG	1 cut(s)	2986
BmuI	ACTGGG	1 cut(s)	2986
BpiI	GAAGAC	4 cut(s)	399, 1055, 1157, 2821
BplI	GAGNNNNNCTC	2 cut(s)	318, 350
BpmI	CTGGAG	3 cut(s)	798, 1229, 1248
Bpu10I	CCTNAGC	1 cut(s)	2580
BpuEI	CTTGAG	2 cut(s)	587, 1452
BpuMI	CCSGG	6 cut(s)	24, 350, 359, 860, 1782, 2903
Bsa29I	ATCGAT	1 cut(s)	887
BsaAI	YACGTR	1 cut(s)	2487
BsaHI	GRCGYC	5 cut(s)	15, 114, 303, 399, 789
BsaWI	WCCGGW	1 cut(s)	2426
Bsc4I	CCNNNNNNNGG	7 cut(s)	123, 265, 1380, 1969, 2743, 2892, 2978
Bse1I	ACTGG	5 cut(s)	838, 1212, 2722, 2981, 3024
Bse3DI	GCAATG	2 cut(s)	841, 2549
BseBI	CCWGG	7 cut(s)	124, 389, 446, 542, 676, 1227, 1970
BseCI	ATCGAT	1 cut(s)	887
BseLI	CCNNNNNNNGG	7 cut(s)	123, 265, 1380, 1969, 2743, 2892, 2978
BseMI	GCAATG	2 cut(s)	841, 2549
BseMII	CTCAG	4 cut(s)	1284, 2136, 2349, 2594
BseNI	ACTGG	5 cut(s)	838, 1212, 2722, 2981, 3024
BseRI	GAGGAG	4 cut(s)	109, 133, 165, 1294
BseSI	GKGCMC	2 cut(s)	422, 1342
BsgI	GTGCAG	1 cut(s)	3023
Bsh1236I	CGCG	3 cut(s)	266, 341, 2661
BshFI	GGCC	7 cut(s)	321, 539, 1373, 1780, 1817, 2015, 2459
BshNI	GGYRCC	2 cut(s)	398, 2411
BshVI	ATCGAT	1 cut(s)	887
BsiHKAI	GWGCWC	2 cut(s)	603, 1687
BsiSI	CCGG	8 cut(s)	24, 349, 359, 859, 939, 1781, 2427, 2902
BslFI	GGGAC	1 cut(s)	341
BslI	CCNNNNNNNGG	7 cut(s)	123, 265, 1380, 1969, 2743, 2892, 2978
BsmAI	GTCTC	7 cut(s)	160, 247, 439, 602, 995, 2117, 2531
BsmBI	CGTCTC	3 cut(s)	160, 247, 439
BsmFI	GGGAC	1 cut(s)	341
BsmI	GAATGC	2 cut(s)	897, 2928
BsnI	GGCC	7 cut(s)	321, 539, 1373, 1780, 1817, 2015, 2459
Bsp1286I	GDGCHC	4 cut(s)	422, 603, 1342, 1687
BspANI	GGCC	7 cut(s)	321, 539, 1373, 1780, 1817, 2015, 2459
BspCNI	CTCAG	4 cut(s)	1285, 2137, 2348, 2593
BspDI	ATCGAT	1 cut(s)	887
BspFNI	CGCG	3 cut(s)	266, 341, 2661
BspHI	TCATGA	3 cut(s)	658, 993, 2631
BspLI	GGNNCC	4 cut(s)	400, 781, 1283, 2413
BspMAI	CTGCAG	6 cut(s)	456, 699, 769, 910, 1432, 1459
BspMI	ACCTGC	1 cut(s)	2410
BspPI	GGATC	5 cut(s)	36, 207, 870, 2702, 2727
BspQI	GCTCTTC	1 cut(s)	1692
BspT107I	GGYRCC	2 cut(s)	398, 2411
BsrDI	GCAATG	2 cut(s)	841, 2549
BsrI	ACTGG	5 cut(s)	838, 1212, 2722, 2981, 3024
BssNI	GRCGYC	5 cut(s)	15, 114, 303, 399, 789
BssSI	CACGAG	1 cut(s)	179
BssT1I	CCWWGG	6 cut(s)	322, 783, 1697, 2460, 2742, 2886
Bst2BI	CACGAG	1 cut(s)	179
Bst2UI	CCWGG	7 cut(s)	124, 389, 446, 542, 676, 1227, 1970
Bst4CI	ACNGT	5 cut(s)	1809, 2116, 2267, 2331, 2862
Bst6I	CTCTTC	2 cut(s)	1692, 2694
BstACI	GRCGYC	5 cut(s)	15, 114, 303, 399, 789
BstAPI	GCANNNNNTGC	5 cut(s)	773, 1265, 1436, 1445, 1643
BstBAI	YACGTR	1 cut(s)	2487
BstC8I	GCNNGC	6 cut(s)	343, 418, 921, 1270, 1353, 1493
BstDEI	CTNAG	6 cut(s)	1088, 1293, 1584, 2145, 2335, 2580
BstENI	CCTNNNNNAGG	1 cut(s)	2741
BstFNI	CGCG	3 cut(s)	266, 341, 2661
BstH2I	RGCGCY	2 cut(s)	365, 402
BstHHI	GCGC	5 cut(s)	364, 401, 647, 1259, 2901
BstMAI	GTCTC	7 cut(s)	160, 247, 439, 602, 995, 2117, 2531
BstNI	CCWGG	7 cut(s)	124, 389, 446, 542, 676, 1227, 1970
BstNSI	RCATGY	2 cut(s)	1641, 2039
BstSFI	CTRYAG	7 cut(s)	452, 695, 765, 906, 1428, 1455, 1758
BstSLI	GKGCMC	2 cut(s)	422, 1342
BstSNI	TACGTA	1 cut(s)	2487
BstUI	CGCG	3 cut(s)	266, 341, 2661
BstV2I	GAAGAC	4 cut(s)	399, 1055, 1157, 2821
BstX2I	RGATCY	1 cut(s)	507
BstYI	RGATCY	1 cut(s)	507
Bsu15I	ATCGAT	1 cut(s)	887
BsuI	GTATCC	2 cut(s)	2434, 2747
BsuRI	GGCC	7 cut(s)	321, 539, 1373, 1780, 1817, 2015, 2459
BsuTUI	ATCGAT	1 cut(s)	887
BtrI	CACGTC	2 cut(s)	283, 481
BtsIMutI	CAGTG	2 cut(s)	1205, 2327
BveI	ACCTGC	1 cut(s)	2410
Cac8I	GCNNGC	6 cut(s)	343, 418, 921, 1270, 1353, 1493
CaiI	CAGNNNCTG	4 cut(s)	1427, 1625, 1969, 2063
CciI	TCATGA	3 cut(s)	658, 993, 2631
CfoI	GCGC	5 cut(s)	364, 401, 647, 1259, 2901
Cfr13I	GGNCC	2 cut(s)	138, 538
ClaI	ATCGAT	1 cut(s)	887
CseI	GACGC	2 cut(s)	23, 122
CsiI	ACCWGGT	1 cut(s)	444
DdeI	CTNAG	6 cut(s)	1088, 1293, 1584, 2145, 2335, 2580
DinI	GGCGCC	1 cut(s)	400
DraI	TTTAAA	2 cut(s)	2367, 2433
DraIII	CACNNNGTG	1 cut(s)	499
DrdI	GACNNNNNNGTC	2 cut(s)	563, 2131
DseDI	GACNNNNNNGTC	2 cut(s)	563, 2131
EaeI	YGGCCR	2 cut(s)	319, 2013
Eam1104I	CTCTTC	2 cut(s)	1692, 2694
EarI	CTCTTC	2 cut(s)	1692, 2694
EciI	GGCGGA	2 cut(s)	136, 2099
Ecl136II	GAGCTC	1 cut(s)	601
Eco105I	TACGTA	1 cut(s)	2487
Eco130I	CCWWGG	6 cut(s)	322, 783, 1697, 2460, 2742, 2886
Eco24I	GRGCYC	1 cut(s)	603
Eco47I	GGWCC	1 cut(s)	138
Eco53kI	GAGCTC	1 cut(s)	601
Eco57I	CTGAAG	2 cut(s)	1380, 2718
EcoICRI	GAGCTC	1 cut(s)	601
EcoNI	CCTNNNNNAGG	1 cut(s)	2741
EcoRI	GAATTC	1 cut(s)	167
EcoRII	CCWGG	7 cut(s)	122, 387, 444, 540, 674, 1225, 1968
EcoT14I	CCWWGG	6 cut(s)	322, 783, 1697, 2460, 2742, 2886
EcoT38I	GRGCYC	1 cut(s)	603
EgeI	GGCGCC	1 cut(s)	400
EheI	GGCGCC	1 cut(s)	400
ErhI	CCWWGG	6 cut(s)	322, 783, 1697, 2460, 2742, 2886
Esp3I	CGTCTC	3 cut(s)	160, 247, 439
FalI	AAGNNNNNCTT	4 cut(s)	365, 397, 1047, 1079
FaqI	GGGAC	1 cut(s)	341
FauI	CCCGC	3 cut(s)	168, 1344, 2654
FblI	GTMKAC	3 cut(s)	306, 639, 838
FriOI	GRGCYC	1 cut(s)	603
FspBI	CTAG	6 cut(s)	987, 2091, 2621, 2718, 2964, 2987
FspI	TGCGCA	1 cut(s)	646
GlaI	GCGC	5 cut(s)	363, 400, 646, 1258, 2900
GsuI	CTGGAG	3 cut(s)	798, 1229, 1248
HaeII	RGCGCY	2 cut(s)	365, 402
HaeIII	GGCC	7 cut(s)	321, 539, 1373, 1780, 1817, 2015, 2459
HapII	CCGG	8 cut(s)	24, 349, 359, 859, 939, 1781, 2427, 2902
HgaI	GACGC	2 cut(s)	23, 122
HhaI	GCGC	5 cut(s)	364, 401, 647, 1259, 2901
Hin1I	GRCGYC	5 cut(s)	15, 114, 303, 399, 789
Hin6I	GCGC	5 cut(s)	362, 399, 645, 1257, 2899
HinP1I	GCGC	5 cut(s)	362, 399, 645, 1257, 2899
HincII	GTYRAC	2 cut(s)	307, 640
HindII	GTYRAC	2 cut(s)	307, 640
HpaII	CCGG	8 cut(s)	24, 349, 359, 859, 939, 1781, 2427, 2902
HphI	GGTGA	6 cut(s)	511, 845, 1028, 1487, 1500, 1729
Hpy166II	GTNNAC	8 cut(s)	307, 426, 640, 839, 2033, 2263, 2596, 2939
Hpy8I	GTNNAC	8 cut(s)	307, 426, 640, 839, 2033, 2263, 2596, 2939
Hpy99I	CGWCG	5 cut(s)	116, 305, 308, 341, 578
HpyAV	CCTTC	9 cut(s)	283, 496, 538, 998, 1116, 1363, 1581, 1770, 2952
HpyCH4III	ACNGT	5 cut(s)	1809, 2116, 2267, 2331, 2862
HpyF3I	CTNAG	6 cut(s)	1088, 1293, 1584, 2145, 2335, 2580
Hsp92I	GRCGYC	5 cut(s)	15, 114, 303, 399, 789
HspAI	GCGC	5 cut(s)	362, 399, 645, 1257, 2899
KasI	GGCGCC	1 cut(s)	398
LguI	GCTCTTC	1 cut(s)	1692
LmnI	GCTCC	7 cut(s)	178, 487, 678, 779, 1107, 1281, 2203
MabI	ACCWGGT	1 cut(s)	444
MaeI	CTAG	6 cut(s)	987, 2091, 2621, 2718, 2964, 2987
MaeIII	GTNAC	9 cut(s)	205, 790, 1127, 1403, 1605, 1809, 1927, 2000, 2110
MfeI	CAATTG	1 cut(s)	879
MflI	RGATCY	1 cut(s)	507
MhlI	GDGCHC	4 cut(s)	422, 603, 1342, 1687
MlsI	TGGCCA	1 cut(s)	2015
MluNI	TGGCCA	1 cut(s)	2015
Mly113I	GGCGCC	1 cut(s)	399
MlyI	GAGTC	3 cut(s)	194, 302, 1220
MmeI	TCCRAC	8 cut(s)	81, 135, 240, 324, 546, 569, 1008, 2731
Mox20I	TGGCCA	1 cut(s)	2015
MroXI	GAANNNNTTC	1 cut(s)	2343
MscI	TGGCCA	1 cut(s)	2015
MseI	TTAA	9 cut(s)	747, 1023, 1161, 1313, 1863, 2366, 2432, 2771, 3028
MslI	CAYNNNNRTG	2 cut(s)	2306, 2601
Msp20I	TGGCCA	1 cut(s)	2015
MspA1I	CMGCKG	1 cut(s)	1142
MspI	CCGG	8 cut(s)	24, 349, 359, 859, 939, 1781, 2427, 2902
MunI	CAATTG	1 cut(s)	879
Mva1269I	GAATGC	2 cut(s)	897, 2928
MvaI	CCWGG	7 cut(s)	124, 389, 446, 542, 676, 1227, 1970
MvnI	CGCG	3 cut(s)	266, 341, 2661
NarI	GGCGCC	1 cut(s)	399
NciI	CCSGG	6 cut(s)	24, 350, 359, 860, 1782, 2903
NlaIV	GGNNCC	4 cut(s)	400, 781, 1283, 2413
NmuCI	GTSAC	6 cut(s)	205, 790, 1127, 1403, 1809, 1927
NsbI	TGCGCA	1 cut(s)	646
NspI	RCATGY	2 cut(s)	1641, 2039
PagI	TCATGA	3 cut(s)	658, 993, 2631
PasI	CCCWGGG	1 cut(s)	541
PciSI	GCTCTTC	1 cut(s)	1692
PcsI	WCGNNNNNNNCGW	4 cut(s)	177, 419, 570, 636
PctI	GAATGC	2 cut(s)	897, 2928
PdmI	GAANNNNTTC	1 cut(s)	2343
PfeI	GAWTC	8 cut(s)	592, 655, 884, 1844, 1949, 2051, 2087, 2960
PflMI	CCANNNNNTGG	2 cut(s)	1380, 1969
PfoI	TCCNGGA	2 cut(s)	348, 858
PleI	GAGTC	3 cut(s)	194, 302, 1219
PluTI	GGCGCC	1 cut(s)	402
PpsI	GAGTC	3 cut(s)	194, 302, 1219
Ppu21I	YACGTR	1 cut(s)	2487
PsiI	TTATAA	3 cut(s)	956, 1751, 2589
Psp124BI	GAGCTC	1 cut(s)	603
Psp6I	CCWGG	7 cut(s)	122, 387, 444, 540, 674, 1225, 1968
PspGI	CCWGG	7 cut(s)	122, 387, 444, 540, 674, 1225, 1968
PspN4I	GGNNCC	4 cut(s)	400, 781, 1283, 2413
PspPI	GGNCC	2 cut(s)	138, 538
PstI	CTGCAG	6 cut(s)	456, 699, 769, 910, 1432, 1459
PstNI	CAGNNNCTG	4 cut(s)	1427, 1625, 1969, 2063
PsuI	RGATCY	1 cut(s)	507
PvuII	CAGCTG	1 cut(s)	1142
RseI	CAYNNNNRTG	2 cut(s)	2306, 2601
SacI	GAGCTC	1 cut(s)	603
SalI	GTCGAC	2 cut(s)	305, 638
SapI	GCTCTTC	1 cut(s)	1692
SaqAI	TTAA	9 cut(s)	747, 1023, 1161, 1313, 1863, 2366, 2432, 2771, 3028
Sau96I	GGNCC	2 cut(s)	138, 538
SchI	GAGTC	3 cut(s)	194, 302, 1220
SduI	GDGCHC	4 cut(s)	422, 603, 1342, 1687
SexAI	ACCWGGT	1 cut(s)	444
SfcI	CTRYAG	7 cut(s)	452, 695, 765, 906, 1428, 1455, 1758
SfoI	GGCGCC	1 cut(s)	400
SinI	GGWCC	1 cut(s)	138
SmiMI	CAYNNNNRTG	2 cut(s)	2306, 2601
SmlI	CTYRAG	2 cut(s)	602, 1467
SmoI	CTYRAG	2 cut(s)	602, 1467
SnaBI	TACGTA	1 cut(s)	2487
SspDI	GGCGCC	1 cut(s)	398
SspMI	CTAG	6 cut(s)	987, 2091, 2621, 2718, 2964, 2987
SstI	GAGCTC	1 cut(s)	603
StyI	CCWWGG	6 cut(s)	322, 783, 1697, 2460, 2742, 2886
TaaI	ACNGT	5 cut(s)	1809, 2116, 2267, 2331, 2862
TauI	GCSGC	2 cut(s)	321, 1454
TfiI	GAWTC	8 cut(s)	592, 655, 884, 1844, 1949, 2051, 2087, 2960
Tru1I	TTAA	9 cut(s)	747, 1023, 1161, 1313, 1863, 2366, 2432, 2771, 3028
Tru9I	TTAA	9 cut(s)	747, 1023, 1161, 1313, 1863, 2366, 2432, 2771, 3028
TscAI	CASTG	2 cut(s)	1212, 2334
TseFI	GTSAC	6 cut(s)	205, 790, 1127, 1403, 1809, 1927
Tsp45I	GTSAC	6 cut(s)	205, 790, 1127, 1403, 1809, 1927
TspGWI	ACGGA	6 cut(s)	142, 193, 229, 398, 2006, 2063
TspRI	CASTG	2 cut(s)	1212, 2334
Van91I	CCANNNNNTGG	2 cut(s)	1380, 1969
VpaK11BI	GGWCC	1 cut(s)	138
XagI	CCTNNNNNAGG	1 cut(s)	2741
XapI	RAATTY	8 cut(s)	167, 1588, 1913, 2170, 2361, 2369, 2514, 2993
XceI	RCATGY	2 cut(s)	1641, 2039
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1825
XmiI	GTMKAC	3 cut(s)	306, 639, 838
XmnI	GAANNNNTTC	1 cut(s)	2343
XspI	CTAG	6 cut(s)	987, 2091, 2621, 2718, 2964, 2987
ZraI	GACGTC	2 cut(s)	304, 790

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.